PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 22590561-8 2012 Moreover, siRNA-mediated knockdown of the CXCL8-target gene Bcl-2 increased the sensitivity of PC3 cells to 5-FU. Fluorouracil 108-112 BCL2 apoptosis regulator Homo sapiens 60-65 22312705-0 2011 MiR-122 increases sensitivity of drug-resistant BEL-7402/5-FU cells to 5-fluorouracil via down-regulation of bcl-2 family proteins. Fluorouracil 71-85 BCL2 apoptosis regulator Homo sapiens 109-114 21375586-10 2011 In addition, ghrelin reversed the 5-FU-induced Bcl-2/Bax protein ratio. Fluorouracil 34-38 BCL2 apoptosis regulator Homo sapiens 47-52 21878904-2 2011 However, 5-FU resistance, mainly caused by the overexpression of antiapoptotic proteins such as Bcl-2, often leads ultimately to treatment failure. Fluorouracil 9-13 BCL2 apoptosis regulator Homo sapiens 96-101 21878904-3 2011 We here investigated the effect of Bcl-2 gene silencing, using small interfering RNA (siRNA) (siBcl-2), on the efficacy of 5-FU in CRC. Fluorouracil 123-127 BCL2 apoptosis regulator Homo sapiens 35-40 21878904-4 2011 Transfection of siBcl-2 by a Lipofectamine2000/siRNA lipoplex effectively downregulated Bcl-2 expression in the DLD-1 cell line (a CRC), resulting in significant cell growth inhibition in vitro upon treatment with 5-FU. Fluorouracil 214-218 BCL2 apoptosis regulator Homo sapiens 18-23 21375586-11 2011 CONCLUSION: Ghrelin inhibits 5-FU-induced apoptosis in colon cancer cells through the regulation of the Bcl-2/Bax protein ratio. Fluorouracil 29-33 BCL2 apoptosis regulator Homo sapiens 104-109 20633010-6 2010 5-FU induced p53 and p21 accumulation together with a decrease in cyclin B1 and Bcl-2 levels in treated keloid fibroblasts. Fluorouracil 0-4 BCL2 apoptosis regulator Homo sapiens 80-85 21403907-9 2011 Moreover, compared with 5-FU alone, the apoptosis of CRC cells treated with DCA and 5-FU was enhanced and demonstrated with the changes of Bcl-2, Bax, and caspase-3 proteins. Fluorouracil 84-88 BCL2 apoptosis regulator Homo sapiens 139-144 22216150-0 2011 Induction of Bcl-2 expression by hepatitis B virus pre-S2 mutant large surface protein resistance to 5-fluorouracil treatment in Huh-7 cells. Fluorouracil 101-115 BCL2 apoptosis regulator Homo sapiens 13-18 22216150-8 2011 Induction of Bcl-2 expression by HBV pre-S2Delta protein resulted in resistance to 5-fluorouracil treatment in colony formation, caspase-3 assay, and cell apoptosis, and can enhance cell death by co-incubation with Bcl-2 inhibitor. Fluorouracil 83-97 BCL2 apoptosis regulator Homo sapiens 13-18 22216150-8 2011 Induction of Bcl-2 expression by HBV pre-S2Delta protein resulted in resistance to 5-fluorouracil treatment in colony formation, caspase-3 assay, and cell apoptosis, and can enhance cell death by co-incubation with Bcl-2 inhibitor. Fluorouracil 83-97 BCL2 apoptosis regulator Homo sapiens 215-220 22216150-10 2011 CONCLUSION/SIGNIFICANCE: Our result demonstrates that HBV pre-S2Delta increased Bcl-2 expression which plays an important role in resistance to 5-fluorouracil-caused cell death. Fluorouracil 144-158 BCL2 apoptosis regulator Homo sapiens 80-85 20691103-12 2010 Respectively, the rate of inhibition of EADM,5- Fu, NVB and DDP were significantly higher in the BCL-2 negative cancer cells than in the BCL-2 positive cancer cells. Fluorouracil 45-50 BCL2 apoptosis regulator Homo sapiens 97-102 22811811-10 2010 CONCLUSION: These findings are the first to demonstrate that high Bax expression is a good prognosticator for patients who underwent surgery alone, and that patient with low Bax/Bcl-2 expression ratio benefit from 5-FU-based adjuvant therapies. Fluorouracil 214-218 BCL2 apoptosis regulator Homo sapiens 178-183 20704765-5 2010 When 5-FU and LY were treated in single and sequential combinations, the expression of p-AKT, p-NFkB, p-p53 and bcl-2 was observed on different concentrations by Western blot analysis. Fluorouracil 5-9 BCL2 apoptosis regulator Homo sapiens 112-117 20704765-12 2010 In sequential combination of 5-FU and LY, the expression of p-p53 was increased and bcl-2 expression was diminished compared to 5-FU alone. Fluorouracil 29-33 BCL2 apoptosis regulator Homo sapiens 84-89 19843160-7 2009 In addition, extracellular-regulated protein kinase 5, nuclear factor-kappaB and Bcl-2 protein expression was down-regulated by miR-143, and further reduced by exposure to 5-fluorouracil. Fluorouracil 172-186 BCL2 apoptosis regulator Homo sapiens 81-86 19903580-5 2010 PG490 and 5-FU treatment induced activated caspase 3 and Bax expression and inhibited Bcl-2 expression. Fluorouracil 10-14 BCL2 apoptosis regulator Homo sapiens 86-91 19810096-6 2010 Bax and Bcl-2 protein expressions in tumor tissue treated with 5-FU were associated with both 5-FU sensitivity and the apoptotic cell count. Fluorouracil 63-67 BCL2 apoptosis regulator Homo sapiens 8-13 19810096-6 2010 Bax and Bcl-2 protein expressions in tumor tissue treated with 5-FU were associated with both 5-FU sensitivity and the apoptotic cell count. Fluorouracil 94-98 BCL2 apoptosis regulator Homo sapiens 8-13 20514446-6 2010 5-FU (400 microM) induced apoptosis in MCF-7 cell monolayer as determined by HO/PI staining, PARP cleavage, p53 induction, Bax induction, and Bcl-2 down-regulation. Fluorouracil 0-4 BCL2 apoptosis regulator Homo sapiens 142-147 19585117-10 2010 Western blotting assay revealed oroxylin A enhanced 5-FU-induced apoptosis in HepG2 cells by elevating the expressions of apoptotic-inducing proteins P53 and cleaved PARP and decreasing the expression of apoptotic-inhibitory proteins COX-2, Bcl-2, and pro-caspase3. Fluorouracil 52-56 BCL2 apoptosis regulator Homo sapiens 241-246 18478476-7 2008 Positive expression rate of Bcl-2 was 80% and the positive cells showed resistance to 5-FU, ADM and MMC. Fluorouracil 86-90 BCL2 apoptosis regulator Homo sapiens 28-33 19000447-12 2008 The inhibition rates of 5-fluorouracil (5-FU), VCR, epirubicin (EADM) and OXA on cancer cells were lower in the group with strong expression of bcl-2 than in that with weak expression of bcl-2 (P<0.05). Fluorouracil 24-38 BCL2 apoptosis regulator Homo sapiens 144-149 19000447-12 2008 The inhibition rates of 5-fluorouracil (5-FU), VCR, epirubicin (EADM) and OXA on cancer cells were lower in the group with strong expression of bcl-2 than in that with weak expression of bcl-2 (P<0.05). Fluorouracil 40-44 BCL2 apoptosis regulator Homo sapiens 144-149 18949393-6 2008 Bcl-2/MMR expression was significantly (p=0.030 for whole series; p=0.018 for the 5-FU-treated cases) related to the response to treatment; tumours with low levels of both Bcl-2 and MMR responded better (n=18/31, 58%) than those with high Bcl-2 and MMR (n=3/16, 18%). Fluorouracil 82-86 BCL2 apoptosis regulator Homo sapiens 0-5 18704308-8 2008 It is concluded that the targeted magnetic nanoparticles containing 5-Fu can improve the chemotherapeutic effect of 5-Fu by decreasing bcl-2 expression, increasing bax expression and inducing apoptosis of the liver cancer cells. Fluorouracil 68-72 BCL2 apoptosis regulator Homo sapiens 135-140 18704308-8 2008 It is concluded that the targeted magnetic nanoparticles containing 5-Fu can improve the chemotherapeutic effect of 5-Fu by decreasing bcl-2 expression, increasing bax expression and inducing apoptosis of the liver cancer cells. Fluorouracil 116-120 BCL2 apoptosis regulator Homo sapiens 135-140 17439414-10 2007 The MTT results demonstrated that Bcl-2 and Bcl-xl transfected cells exhibited increased sensitivity to 5-FU or HCPT. Fluorouracil 104-108 BCL2 apoptosis regulator Homo sapiens 34-39 18082672-7 2008 Mechanistic studies demonstrated that the 5-FU plus scFv23/TNF combination specifically resulted in a down-regulation of HER-2/neu, p-Akt and Bcl-2 and up-regulation of TNF-R1. Fluorouracil 42-46 BCL2 apoptosis regulator Homo sapiens 142-147 17537404-4 2007 However, tumor cells that adapt to oxidative stress by increasing manganese superoxide dismutase (MnSOD), Prx I, and Bcl-2 showed drug resistance to 5-FU. Fluorouracil 149-153 BCL2 apoptosis regulator Homo sapiens 117-122 15996812-2 2006 The present work reports the involvement of Bcl-2 in response to the exposure of HEp-2 and KB cells to Carb or 5-FU. Fluorouracil 111-115 BCL2 apoptosis regulator Homo sapiens 44-49 16557594-6 2006 High dose 5-FU also induced a higher regulation of the mitochondrial death genes APAF1, BAK1 and BCL2, and induction of genes of the ID family. Fluorouracil 10-14 BCL2 apoptosis regulator Homo sapiens 97-101 16407826-0 2006 Overexpression of hRFI inhibits 5-fluorouracil-induced apoptosis in colorectal cancer cells via activation of NF-kappaB and upregulation of BCL-2 and BCL-XL. Fluorouracil 32-46 BCL2 apoptosis regulator Homo sapiens 140-145 17287894-5 2006 Reverse transcription-polymerase chain reaction analysis revealed that exposure to 5-FU downregulated both MDR1 and bcl-2 mRNA and simultaneously upregulated CE2 mRNA expression, suggesting enhancement of subsequent CPT-11 cytotoxicity. Fluorouracil 83-87 BCL2 apoptosis regulator Homo sapiens 116-121 16596200-0 2006 hRFI overexpressed in HCT116 cells modulates Bcl-2 family proteins when treated with 5-fluorouracil. Fluorouracil 85-99 BCL2 apoptosis regulator Homo sapiens 45-50 16596200-5 2006 As a result, we identified four genes (Bcl-2, Bcl-XL, cIAP2, and CFLAR) whose expression was four or more times higher in HCT116/ hRFI cells than in HCT116/LacZ cells, and found that Bcl-2 and the ratio of Bcl-2/Bax or Bcl-2/Bak were upregulated when HCT116/hRFI cells were treated with 5-FU. Fluorouracil 287-291 BCL2 apoptosis regulator Homo sapiens 39-44 16596200-6 2006 Furthermore, we also validated the up-regulation of Bcl-2 and Bcl-XL in HCT116/hRFI cells treated with 5-FU by Western blot analysis. Fluorouracil 103-107 BCL2 apoptosis regulator Homo sapiens 52-57 16596200-7 2006 Such evidence suggests that the modulation of Bcl-2 family proteins seen in 5-FU treatment plays an important role in the anti-apoptotic function of HCT116/hRFI cells. Fluorouracil 76-80 BCL2 apoptosis regulator Homo sapiens 46-51 16391804-8 2006 Microarray analysis revealed that expressions of some apoptosis related genes such as Bcl-2 changed, and were correlated with sequence-dependent cytotoxicity of the 5-FU --> CPT-11 sequence. Fluorouracil 165-169 BCL2 apoptosis regulator Homo sapiens 86-91 16412276-0 2006 Inhibition of Bcl-2 expression by a novel tumor-specific RNA interference system increases chemosensitivity to 5-fluorouracil in Hela cells. Fluorouracil 111-125 BCL2 apoptosis regulator Homo sapiens 14-19 16412276-12 2006 Inhibition of Bcl-2 did not affect cell proliferation, but increased the chemosensitivity of HeLa cells to 5-fluorouracil. Fluorouracil 107-121 BCL2 apoptosis regulator Homo sapiens 14-19 16412276-14 2006 Suppression of Bcl-2 by using this system sensitized HeLa cells to 5-fluorouracil. Fluorouracil 67-81 BCL2 apoptosis regulator Homo sapiens 15-20 15467752-0 2004 Multidomain Bcl-2 homolog Bax but not Bak mediates synergistic induction of apoptosis by TRAIL and 5-FU through the mitochondrial apoptosis pathway. Fluorouracil 99-103 BCL2 apoptosis regulator Homo sapiens 12-17 16603448-0 2006 Bcl-2 siRNA induced apoptosis and increased sensitivity to 5-fluorouracil and HCPT in HepG2 cells. Fluorouracil 59-73 BCL2 apoptosis regulator Homo sapiens 0-5 16603448-7 2006 Accordingly, Bax protein expression had no change and caspase-3 protein expression showed significantly be up regulated; (2) MTT results showed that Bcl-2 siRNA transfectants had higher cell inhibitory rates after treated with 5-FU or HCPT; (3) flow cytometry results demonstrated that sub G1 population increased in Bcl-2 siRNA transfected cells compared with negative siRNA or untreated cells. Fluorouracil 227-231 BCL2 apoptosis regulator Homo sapiens 149-154 16603448-8 2006 After addition 5-FU (1300 mg/l) and HCPT (0.72 mg/l), Bcl-2 siRNA cells showed higher sub G1 population than negative siRNA or untreated cells. Fluorouracil 15-19 BCL2 apoptosis regulator Homo sapiens 54-59 16603448-9 2006 siRNA targeting Bcl-2 gene can specifically down-regulate Bcl-2 expression, increased Bax/Bcl-2 ratio expression and caspase-3 activity in HepG2 cells, which lead to increase cells spontaneous apoptosis and sensitize cells to 5-FU or HCPT. Fluorouracil 226-230 BCL2 apoptosis regulator Homo sapiens 16-21 15365767-9 2005 DHA markedly increased the inhibitory effect of 5-FU on the expression of the antiapoptotic proteins BCL-2 and BCL-XL, and induced overexpression of c-MYC which has recently been shown to drive apoptosis and, when overexpressed, to sensitize cancer cells to the action of proapoptotic agents, including 5-FU. Fluorouracil 48-52 BCL2 apoptosis regulator Homo sapiens 101-106 16332177-0 2005 Enhanced sensitivity of human hepatoma cells to 5-fluorouracil by small interfering RNA targeting Bcl-2. Fluorouracil 48-62 BCL2 apoptosis regulator Homo sapiens 98-103 16332177-1 2005 This study was designed to reveal whether the apoptosis induced in human hepatocellular carcinoma (HCC) cell lines by 5-fluorouracil (5-FU) could be enhanced by transfecting Bcl-2 small interfering RNA (siRNA). Fluorouracil 118-132 BCL2 apoptosis regulator Homo sapiens 174-179 16332177-1 2005 This study was designed to reveal whether the apoptosis induced in human hepatocellular carcinoma (HCC) cell lines by 5-fluorouracil (5-FU) could be enhanced by transfecting Bcl-2 small interfering RNA (siRNA). Fluorouracil 134-138 BCL2 apoptosis regulator Homo sapiens 174-179 16332177-2 2005 Bcl-2 siRNA and control siRNA were transfected into cells following treatment with or without 5-FU. Fluorouracil 94-98 BCL2 apoptosis regulator Homo sapiens 0-5 16332177-5 2005 Incubation of all cell lines with Bcl-2 siRNA reduced cell viability 96 h after 5-FU treatment compared with all other controls: Huh-7 (P < 0.01), Huh-7 with hepatitis C replicon (P < 0.01), HepG2 (P < 0.01), HLE (P < 0.05). Fluorouracil 80-84 BCL2 apoptosis regulator Homo sapiens 34-39 16332177-7 2005 The Bcl-2 siRNA prior to 5-FU treatment group showed the strongest effect of inducing apoptosis. Fluorouracil 25-29 BCL2 apoptosis regulator Homo sapiens 4-9 16168113-9 2005 The sequence Dox-->Pacl-->48-h washout-->5-FU produced a synergistic and highly schedule-dependent interaction (combination index < 1), resulting in an induction of apoptosis in both experimental models regardless of hormonal, p53, bcl-2 or bax status. Fluorouracil 50-54 BCL2 apoptosis regulator Homo sapiens 244-249 11920608-0 2002 Resistance of colon cancer cells to long-term 5-fluorouracil exposure is correlated to the relative level of Bcl-2 and Bcl-X(L) in addition to Bax and p53 status. Fluorouracil 46-60 BCL2 apoptosis regulator Homo sapiens 109-114 15124184-7 2004 We also detected the active form of caspase-3 and Bid in apoptotic leukemia cells after treatment with 5-FU and/or anti-CD8 antibody, indicating that the drug and antibody induced cell death through caspase-3 and the signal pathway may involve the Bcl-2 protein family. Fluorouracil 103-107 BCL2 apoptosis regulator Homo sapiens 248-253 12915131-5 2003 These results indicate that Ras, Bcl-2, as well as Raf-1 and PI3K pathways play pivotal roles in 5-FU-induced apoptosis under Ha-ras-overexpressed condition. Fluorouracil 97-101 BCL2 apoptosis regulator Homo sapiens 33-38 12915131-8 2003 Through understanding the mechanism of 5-FU induced apoptosis in tumor cells, a new direction toward the treatment of Ha-ras oncogene-related cancers with 5-FU at more optimal dosages is possible and combinational therapy with other drugs that suppress PI3K and Bcl-2 activities can also be considered. Fluorouracil 39-43 BCL2 apoptosis regulator Homo sapiens 262-267 12469202-1 2003 Bcl-2 in cancer cells was shown to be a potent indicator of 5-FU efficacy, but the protein in normal tissue cells appeared not to be a marker of 5-FU toxicity probably due to the functional alteration of Bcl-2 associated with cell senescence. Fluorouracil 60-64 BCL2 apoptosis regulator Homo sapiens 0-5 15177426-1 2004 This study was designed to observe whether the rates of apoptosis induced in the breast cancer cell line MCF-7 by 5-fluorouracil (5-FU) could be enhanced by transfecting bcl-2 antisense oligonucleotide (ASODN). Fluorouracil 114-128 BCL2 apoptosis regulator Homo sapiens 170-175 15177426-1 2004 This study was designed to observe whether the rates of apoptosis induced in the breast cancer cell line MCF-7 by 5-fluorouracil (5-FU) could be enhanced by transfecting bcl-2 antisense oligonucleotide (ASODN). Fluorouracil 130-134 BCL2 apoptosis regulator Homo sapiens 170-175 15177426-5 2004 Moreover, incubation of MCF-7 with bcl-2 ASODN prior to 5-FU treatment caused remarkable loss of viable cells compared with all other control ODNs (P < 0.01). Fluorouracil 56-60 BCL2 apoptosis regulator Homo sapiens 35-40 12469202-0 2003 Bcl-2 in cancer and normal tissue cells as a prediction marker of response to 5-fluorouracil. Fluorouracil 78-92 BCL2 apoptosis regulator Homo sapiens 0-5 11920608-6 2002 In addition, we found that high levels of anti-apoptotic Bcl-2 and Bcl-x(L) proteins combined with a low level of Bax were correlated to high 5-FU resistance of wild-type p53 cell lines. Fluorouracil 142-146 BCL2 apoptosis regulator Homo sapiens 57-62 11920608-8 2002 In conclusion, the relative levels of Bcl-2, Bcl-x(L) and Bax may altogether contribute to determine the resistance of a majority of colon tumor cells to long-term 5-FU treatment, whatever their p53 status. Fluorouracil 164-168 BCL2 apoptosis regulator Homo sapiens 38-43 11044365-9 2000 Moreover, after 5-FU exposure, Bax and Bcl-2 proteins regulation was under p53 protein control and a statistically significant relationship (r = 0.880, P = 0.0097) was observed between Bcl-2/Bax ratio and 5-FU sensitivity. Fluorouracil 16-20 BCL2 apoptosis regulator Homo sapiens 39-44 12138244-3 2002 METHODS: The cytotoxic effects of 5-FU and gemcitabine in AsPC-1, Capan-1, Mia-PaCa-2 and T3M4 pancreatic cancer cell lines were assessed by growth assays, and mRNA expression levels of pro-apoptotic and anti-apoptotic genes of the Bcl-2 family were analyzed by RNAse protection assays. Fluorouracil 34-38 BCL2 apoptosis regulator Homo sapiens 232-237 12138244-8 2002 The bax/bcl-2 ratio maintained relatively stable following 5-FU/gemcitabine treatment and reflected the chemotherapeutic sensitivity of these cell lines. Fluorouracil 59-63 BCL2 apoptosis regulator Homo sapiens 8-13 11044365-0 2000 Bcl-2/Bax protein ratio predicts 5-fluorouracil sensitivity independently of p53 status. Fluorouracil 33-47 BCL2 apoptosis regulator Homo sapiens 0-5 11044365-9 2000 Moreover, after 5-FU exposure, Bax and Bcl-2 proteins regulation was under p53 protein control and a statistically significant relationship (r = 0.880, P = 0.0097) was observed between Bcl-2/Bax ratio and 5-FU sensitivity. Fluorouracil 16-20 BCL2 apoptosis regulator Homo sapiens 185-190 11044365-9 2000 Moreover, after 5-FU exposure, Bax and Bcl-2 proteins regulation was under p53 protein control and a statistically significant relationship (r = 0.880, P = 0.0097) was observed between Bcl-2/Bax ratio and 5-FU sensitivity. Fluorouracil 205-209 BCL2 apoptosis regulator Homo sapiens 39-44 11044365-9 2000 Moreover, after 5-FU exposure, Bax and Bcl-2 proteins regulation was under p53 protein control and a statistically significant relationship (r = 0.880, P = 0.0097) was observed between Bcl-2/Bax ratio and 5-FU sensitivity. Fluorouracil 205-209 BCL2 apoptosis regulator Homo sapiens 185-190 11044365-10 2000 In conclusion, whatever p53 status, Bcl-2 or Bax induction and Bcl-2/Bax protein ratio were correlated to 5-FU sensitivity. Fluorouracil 106-110 BCL2 apoptosis regulator Homo sapiens 36-41 11044365-10 2000 In conclusion, whatever p53 status, Bcl-2 or Bax induction and Bcl-2/Bax protein ratio were correlated to 5-FU sensitivity. Fluorouracil 106-110 BCL2 apoptosis regulator Homo sapiens 63-68 10697613-6 1999 CONCLUSION: The present results suggest that TP is important for remodeling the existing vasculature early in tumor development and intraductal extension, expressions of TP and Bcl-2 are tightly linked and TP status can not generally predict chemotherapeutic sensitivity for 5-FU as a single molecular marker. Fluorouracil 275-279 BCL2 apoptosis regulator Homo sapiens 177-182 10499633-8 1999 In addition, biochemical examination revealed that 5-FU and HU blocked the antimitotic agent-induced increase of p21WAF1/CIP1 protein levels, as well as prevented the hyperphosphorylation of the bcl-2 and c-raf-1 proteins. Fluorouracil 51-55 BCL2 apoptosis regulator Homo sapiens 195-200 10470656-10 1999 The BCL-2/BAX mRNA ratio was decreased in response to 5-FU in IMR90. Fluorouracil 54-58 BCL2 apoptosis regulator Homo sapiens 4-9 9815973-11 1995 In the cyclophosphamide-methotrexate-fluorouracil-treated group, bcl-2 absence was significant for poor overall survival (P = 0.02) as well as a number of nodes above 3 (P = 0.04) and a tumor size above 2 cm (P = 0.05). Fluorouracil 37-49 BCL2 apoptosis regulator Homo sapiens 65-70 9792140-0 1998 5-Fluorouracil induces apoptosis in human colon cancer cell lines with modulation of Bcl-2 family proteins. Fluorouracil 0-14 BCL2 apoptosis regulator Homo sapiens 85-90 9792140-3 1998 We sought to determine the roles played by the p53 and Bcl-2 family of proteins in 5-fluorouracil (5-FU)-induced apoptosis of human colon cancer cell lines. Fluorouracil 83-97 BCL2 apoptosis regulator Homo sapiens 55-60 9792140-3 1998 We sought to determine the roles played by the p53 and Bcl-2 family of proteins in 5-fluorouracil (5-FU)-induced apoptosis of human colon cancer cell lines. Fluorouracil 99-103 BCL2 apoptosis regulator Homo sapiens 55-60 9792140-11 1998 In cells containing wild-type p53 (e.g. LoVo), 5-FU-induced apoptosis was accompanied by increased expression of Bax and Bak without consistent modulation of other bcl-2 family proteins. Fluorouracil 47-51 BCL2 apoptosis regulator Homo sapiens 164-169 9792140-14 1998 In conclusion, these results suggest that some members of the Bcl-2 family of proteins, in human colon cancer cell lines, are modulated by 5-FU and that the ratio of Bcl-X(L) to Bax may be related to chemosensitivity to 5-FU. Fluorouracil 139-143 BCL2 apoptosis regulator Homo sapiens 62-67 9792140-14 1998 In conclusion, these results suggest that some members of the Bcl-2 family of proteins, in human colon cancer cell lines, are modulated by 5-FU and that the ratio of Bcl-X(L) to Bax may be related to chemosensitivity to 5-FU. Fluorouracil 220-224 BCL2 apoptosis regulator Homo sapiens 62-67 11819336-1 1998 AIM:To compare the expression level of Fas gene and Bcl-2 gene in gastric cancer cells SGC7901 and gastric cancer MDR (multidrug resistant) cells SGC7901/VCR,to transduce Fas cDNA and Bcl-2 antisense nucleic acid into SGC7901/VCR cells respectively, and to observe the expression of two genes in transfectants and non-transfectants as well as their drug sensitivity.METHODS:Eukaryotic expression vector pBK-Fas cDNA and pDOR-anti Bcl-2 were constructed and transfected into SGC7901/VCR cells by lipofectamine,respectively.Northern blot and Western blot were used to detect the expression of mRNA and protein in SGC7901/VCR and SGC7901 cells and transfectants, and drug sensitivity of transfectants for VCR, CDDP and 5-FU was analyzed with MTT assay.RESULTS:After gene transfection, 80 for Fas and 120 for antisense Bcl-2 drug-resistant clones were selected from 2 X10(5) cells, transfection rate being 0.04% and 0.06%. Fluorouracil 716-720 BCL2 apoptosis regulator Homo sapiens 52-57 14646558-7 1997 The Bax/Bcl-2 expression ratio was gradually elevated for up to 72 h in MKN-74 and MKN-45 cells treated with 1mM 5-FU; in contrast, it was unchanged in MKN-28 and KATO-III cells, and MKN-74 treated with 50 microM 5-FU. Fluorouracil 113-117 BCL2 apoptosis regulator Homo sapiens 8-13 14646558-7 1997 The Bax/Bcl-2 expression ratio was gradually elevated for up to 72 h in MKN-74 and MKN-45 cells treated with 1mM 5-FU; in contrast, it was unchanged in MKN-28 and KATO-III cells, and MKN-74 treated with 50 microM 5-FU. Fluorouracil 213-217 BCL2 apoptosis regulator Homo sapiens 8-13 14646558-8 1997 These results might indicate that (1) 1mM 5-FU induces apoptosis in cultured gastric cancer cells carrying the wild-type p53 gene, but not those carrying the mutated type or a gene deletion, and (2) the elevated Bax/Bcl-2 expression ratio plays a more crucial role than the higher expression of P21/Waf1 in the induction of p53- gene dependent apoptosis. Fluorouracil 42-46 BCL2 apoptosis regulator Homo sapiens 216-221 9020491-3 1997 We analysed bcl-2 expression in 231 colorectal tumours from patients that were treated by surgery alone or with 5-fluorouracil-based chemotherapy. Fluorouracil 112-126 BCL2 apoptosis regulator Homo sapiens 12-17 9209521-6 1997 There was an inverse relationship between bcl-2 oncoprotein expression and apoptosis in 5-FU-treated patients, but no significant correlation between histological effect and apoptosis. Fluorouracil 88-92 BCL2 apoptosis regulator Homo sapiens 42-47 9209521-8 1997 CONCLUSIONS: Apoptosis may be induced by 5-FU administered preoperatively and bcl-2 oncogene expression may suppress 5-FU-induced apoptosis. Fluorouracil 117-121 BCL2 apoptosis regulator Homo sapiens 78-83 9020491-7 1997 In the group of patients receiving 5-fluorouracil-based chemotherapy bcl-2 expression did not influence response to chemotherapy; nor did it effect failure-free or overall survival. Fluorouracil 35-49 BCL2 apoptosis regulator Homo sapiens 69-74 34485154-5 2021 Our results show that ASP inhibited 5-FU-induced the decrease in Bcl-2 protein and the increase in Bax protein. Fluorouracil 36-40 BCL2 apoptosis regulator Homo sapiens 65-70 7654318-3 1994 Using the phosphatase inhibitor okadaic acid (OA) or chemotherapeutic agents such as Taxol and 5"-fluorouracil, we found that bcl-2 can be phosphorylated. Fluorouracil 95-110 BCL2 apoptosis regulator Homo sapiens 126-131 7654318-8 1994 Treatment with the phosphatase inhibitor or with chemotherapeutic agents (Taxol, 5"-fluorouracil) led to severe apoptosis of these cells, along with hyperphosphorylation of bcl-2. Fluorouracil 81-96 BCL2 apoptosis regulator Homo sapiens 173-178 34970530-8 2021 Mechanistically, 2b induced apoptosis-dependent anticancer effect (43%) higher than that of 5-fluorouracil (34.95%) in three studied cancer cell lines, activated p53 (47%), downregulated the Bcl2 gene (1.25-fold), and upregulated p21 (2-fold) in the treated cancer cells. Fluorouracil 92-106 BCL2 apoptosis regulator Homo sapiens 191-195 34794098-7 2021 The most balanced potent and safe derivatives; 5i and 5q were more active than 5-fluorouracil, exhibited low mumrange MDM2 binding (KD=1.32and 1.72 mum, respectively), induced apoptosis-dependent anticancer activities up to 50%, activated p53 by 47-63%, downregulated the BCL2 gene to 59.8%, and reduced its protein level (13.75%) in the treated cancer cells. Fluorouracil 79-93 BCL2 apoptosis regulator Homo sapiens 272-276 34637097-6 2021 METHOD AND RESULTS: Different single and combined concentrations of 5-FU and Quer were applied to MCF 7 cells for 48 h. Cell viability, apoptosis, gene expression of Bax, Bcl2, and p53, caspase activity, and colony number were assessed using MTT assay, flow cytometry, quantitative real-time PCR, enzyme-linked immunosorbent (ELISA), and Colony formation assay, respectively. Fluorouracil 68-72 BCL2 apoptosis regulator Homo sapiens 171-175 34637097-7 2021 The combination of 5-FU and Quer compared to 5-FU alone improved apoptosis by increasing the gene expression of Bax and p53 and caspase-9 activity and decreasing the Bcl2 gene expression. Fluorouracil 19-23 BCL2 apoptosis regulator Homo sapiens 166-170 34637097-7 2021 The combination of 5-FU and Quer compared to 5-FU alone improved apoptosis by increasing the gene expression of Bax and p53 and caspase-9 activity and decreasing the Bcl2 gene expression. Fluorouracil 45-49 BCL2 apoptosis regulator Homo sapiens 166-170 34795760-0 2021 Nrf3 Promotes 5-FU Resistance in Colorectal Cancer Cells via the NF-kappaB/BCL-2 Signaling Pathway In Vitro and In Vivo. Fluorouracil 14-18 BCL2 apoptosis regulator Homo sapiens 75-80 34117917-5 2021 RESULTS: Our results demonstrated that co-administration of HPRP-A1 with iRGD increased the apoptosis, while these two peptides in combination with 5FU increased the intracellular level of p53 that upregulate the pro-apoptotic gene BAX and downregulate the anti-apoptotic gene BCL2. Fluorouracil 148-151 BCL2 apoptosis regulator Homo sapiens 277-281 34683888-6 2021 The expression levels of E2F2 and Bcl-2 genes were found to be suppressed after treatment with both WO3-Cys and WO3-Trp NPs more than 5-FU-treated cells. Fluorouracil 134-138 BCL2 apoptosis regulator Homo sapiens 34-39 34309458-10 2022 In addition, when the anti-apoptotic Bcl-2, mTOR, and Akt gene expression levels were normalized as 1 in the control group, they were 0.28, 0.41, and 0.22 with 5-FU + L treatment, and 0.32, 0.46, and 0.39, respectively, with 5-FU + Q treatment. Fluorouracil 160-164 BCL2 apoptosis regulator Homo sapiens 37-42 34309458-10 2022 In addition, when the anti-apoptotic Bcl-2, mTOR, and Akt gene expression levels were normalized as 1 in the control group, they were 0.28, 0.41, and 0.22 with 5-FU + L treatment, and 0.32, 0.46, and 0.39, respectively, with 5-FU + Q treatment. Fluorouracil 225-229 BCL2 apoptosis regulator Homo sapiens 37-42 35416117-0 2022 Discoidin domain receptor 1a (DDR1a) confers 5-fluorouracil cytotoxicity in LoVo cell via PI3K/AKT/Bcl-2 pathway. Fluorouracil 45-59 BCL2 apoptosis regulator Homo sapiens 99-104 34367280-11 2021 We also found that downregulation of ECT2 increased 5-FU sensitivity in GC cells by downregulating P-gp, MRP1, and Bcl-2. Fluorouracil 52-56 BCL2 apoptosis regulator Homo sapiens 115-120 35154466-6 2022 Meanwhile, CP-25 and 5-Fu activated the intrinsic mitochondrial apoptosis pathway induced by P53, inhibited anti-apoptotic B-cell lymphoma (Bcl-2), induced the pro-apoptotic Bcl-2-associated X protein (Bax), Cytochrome-C and caspases. Fluorouracil 21-25 BCL2 apoptosis regulator Homo sapiens 140-145 35326689-11 2022 Moreover, 5-FU/VD3/Met revealed maximal inhibitions of cell cycle inducers (CCND1/CCND3), cell survival (BCL2), and the PI3K/Akt/mTOR molecules alongside the highest expression of cell cycle inhibitors (p21/p27), proapoptotic markers (BAX/cytochrome C/caspase-3), and PTEN in both cell lines. Fluorouracil 10-14 BCL2 apoptosis regulator Homo sapiens 105-109 33662352-5 2021 The use of 5-FU and L-OHP, either alone or in combination, strongly suppressed Akt activation, Survivin, Bcl-2, and Bcl-xL expression, and enhanced Puma, phospho-p53, and p53 expression in Caco-2 cells than in DLD-1 cells. Fluorouracil 11-15 BCL2 apoptosis regulator Homo sapiens 105-110 30056083-3 2018 Compared to 5-FU alone, MH synergistically enhanced the chemotherapeutic effects of 5-FU, by reducing cell proliferation through the suppression of EGFR, HER2, p-Akt and p-mTOR expression, and promoting apoptosis by the modulation pro-apoptotic (p53, Bax, Cyto c, FasL caspase-3, -8, -9 and cleave-PARP) and anti-apoptotic (Bcl-2) markers. Fluorouracil 84-88 BCL2 apoptosis regulator Homo sapiens 324-329 33460610-5 2021 Furthermore, Nanog knockdown significantly increased CRC cell sensitivity to 5-FU drug via modulating Bax and Bcl-2 mRNA expression. Fluorouracil 77-81 BCL2 apoptosis regulator Homo sapiens 110-115 30990333-3 2021 The combination of PGG and 5-FU had synergistic effects on reversal the aggressive phenotypes of HepG2 cells, increasing the proportion of Bax/Bcl-2, promoting the activation of caspase-9 and caspase-3, and inducing apoptosis. Fluorouracil 27-31 BCL2 apoptosis regulator Homo sapiens 143-148 33402109-11 2021 Overexpression of miR-383 downregulated Bcl-2, resulting in reduced survival of Fluorouracil-treated GC cells. Fluorouracil 80-92 BCL2 apoptosis regulator Homo sapiens 40-45 33402109-13 2021 CONCLUSION: MiR-383 reduces survival of Fluorouracil-treated GC cells through downregulating of Bcl-2. Fluorouracil 40-52 BCL2 apoptosis regulator Homo sapiens 96-101 33389946-4 2020 Moreover, the combined 5-FU/PCA exposure led to upregulation of p53 and downregulation of Bcl-2 protein when compared to the untreated control cells. Fluorouracil 23-27 BCL2 apoptosis regulator Homo sapiens 90-95 33389946-6 2020 The mechanisms by which the combined 5-FU/PCA exposure exerts its effects are associated with upregulation of p53 gene expression and downregulation of Bcl-2 level. Fluorouracil 37-41 BCL2 apoptosis regulator Homo sapiens 152-157 33389946-7 2020 Therefore, the combination of 5-FU with PCA not only could be a promising approach to potentially reduce the dose requirements of 5-FU but also could promote apoptosis via p53 and Bcl-2 signaling pathways. Fluorouracil 30-34 BCL2 apoptosis regulator Homo sapiens 180-185 33193866-9 2020 Bcl-2 siRNA corona successfully entered the cytosol of Bel7402/5-FU MDR cells to downregulate Bcl-2 protein levels in vitro and in vivo. Fluorouracil 63-67 BCL2 apoptosis regulator Homo sapiens 0-5 33193866-9 2020 Bcl-2 siRNA corona successfully entered the cytosol of Bel7402/5-FU MDR cells to downregulate Bcl-2 protein levels in vitro and in vivo. Fluorouracil 63-67 BCL2 apoptosis regulator Homo sapiens 94-99 33193866-10 2020 Bcl-2 siRNA/Dox-TPGS-LPs showed superior to TPGS- (or siRNA-) linked Dox liposomes in cell apoptosis and cytotoxicity assay in Bel7402/5-FU MDR cells, and 7-fold greater effect than free Dox in tumor growth inhibition of Bel7402/5-FU xenograft nude mice. Fluorouracil 135-139 BCL2 apoptosis regulator Homo sapiens 0-5 33193866-10 2020 Bcl-2 siRNA/Dox-TPGS-LPs showed superior to TPGS- (or siRNA-) linked Dox liposomes in cell apoptosis and cytotoxicity assay in Bel7402/5-FU MDR cells, and 7-fold greater effect than free Dox in tumor growth inhibition of Bel7402/5-FU xenograft nude mice. Fluorouracil 229-233 BCL2 apoptosis regulator Homo sapiens 0-5 32509181-12 2020 In addition, the combination treatment (emodin plus 5-Fu) induced cell apoptosis via inhibiting Bcl-2 and activating cleaved caspase3 and Bax. Fluorouracil 52-56 BCL2 apoptosis regulator Homo sapiens 96-101 31736281-5 2020 Of these genes, ABCG2, AHNAK2, BCL2, FZD1, and TP73 are associated with published evidence for resistance to 5-fluorouracil and cisplatin. Fluorouracil 109-123 BCL2 apoptosis regulator Homo sapiens 31-35 31544060-5 2019 The cytotoxic effects, the number of colonies, apoptosis, p53 gene expression, and Bcl-2 signaling protein of the combined 5-FU and beta-escin on MCF7 cells were determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, clonogenic assay, flow cytometry, real-time quantitative polymerase chain reaction, and western blotting methods, respectively. Fluorouracil 123-127 BCL2 apoptosis regulator Homo sapiens 83-88 31544060-9 2019 In addition, the number of colonies and Bcl-2 signaling protein in combination of 5-FU and beta-escin decreased with respect to untreated control cells or single treatment of 5-FU and beta-escin. Fluorouracil 82-86 BCL2 apoptosis regulator Homo sapiens 40-45 31544060-9 2019 In addition, the number of colonies and Bcl-2 signaling protein in combination of 5-FU and beta-escin decreased with respect to untreated control cells or single treatment of 5-FU and beta-escin. Fluorouracil 175-179 BCL2 apoptosis regulator Homo sapiens 40-45 31544060-10 2019 The combination of 5-FU and beta-escin not only has synergistic effects by increasing cell apoptosis and p53 gene expression but also decreases Bcl-2 signaling protein in MCF7 cell lines. Fluorouracil 19-23 BCL2 apoptosis regulator Homo sapiens 144-149 31592435-10 2019 PC3 cell colony counts and Bcl-2 signaling protein were decreased by 5-FU/rutin combination. Fluorouracil 69-73 BCL2 apoptosis regulator Homo sapiens 27-32 31592435-11 2019 Conclusion: Synergistic effects of 5-FU/rutin combination on PC3 cells line enhanced apoptosis, p53 gene expression, and down-regulation of Bcl-2 protein, compared to control separate application. Fluorouracil 35-39 BCL2 apoptosis regulator Homo sapiens 140-145 31072625-12 2019 Additionally, transfection of miR-145 mimic further altered expression of key genes involved in cell apoptosis (Bcl-2, Bax, Caspase3) in ESCC cells treated with 5-FU. Fluorouracil 161-165 BCL2 apoptosis regulator Homo sapiens 112-117 30741544-9 2019 The accumulation of 5-FU resulted in caspase-3 and PARP activation, Bcl-2 reduction, and Bad increase, which ultimately lead to cancer cell apoptosis. Fluorouracil 20-24 BCL2 apoptosis regulator Homo sapiens 68-73 33335403-6 2020 The expression of some apoptotic and autophagy-related genes such as Bax, Bcl2, Beclin1 and ATG7 was significantly induced by carbon-ion beam irradiation alone and was further enhanced when the beam was combined with 5-FU. Fluorouracil 217-221 BCL2 apoptosis regulator Homo sapiens 74-78 32975155-3 2020 Results: Combination treatment with 5-FU and radiation had a stronger effect on decreasing Bcl-2 expression and increasing expression of Bax, cleaved caspase-9, cleaved caspase-3, cleaved PARP compared with each treatment alone. Fluorouracil 36-40 BCL2 apoptosis regulator Homo sapiens 91-96 32247577-7 2020 BOK, a BCL-2 family protein thought comparable to multidomain pro-apoptotic proteins BAX and BAK, has recently been identified as a key player in metabolism of and resistance to the commonly used chemotherapeutic 5-FU. Fluorouracil 213-217 BCL2 apoptosis regulator Homo sapiens 7-12 30993753-6 2019 Our data from gene expression determined a substantial diminish in the mRNA levels of the Nrf2 and antiapoptotic gene Bcl-2 along with a noticeable increase in the level of the proapoptotic Bax in HT-29 colon cells that underwent cotreatment with 5-FU and stattic (P < 0.05). Fluorouracil 247-251 BCL2 apoptosis regulator Homo sapiens 118-123 31949673-0 2018 Knockdown of PHGDH potentiates 5-FU cytotoxicity in gastric cancer cells via the Bcl-2/Bax/caspase-3 signaling pathway. Fluorouracil 31-35 BCL2 apoptosis regulator Homo sapiens 81-86 31949673-12 2018 Additionally, 5-FU treatment downregulated Bcl-2 expression and upregulated the expression of Bax and caspase-3, and this effect was remarkably enhanced by PHGDH knockdown. Fluorouracil 14-18 BCL2 apoptosis regulator Homo sapiens 43-48 31949673-13 2018 In conclusion, knockdown of PHGDH potentiates 5-FU cytotoxicity in GC cells via the Bcl-2/Bax/caspase-3 signaling pathway. Fluorouracil 46-50 BCL2 apoptosis regulator Homo sapiens 84-89 29738772-5 2018 Interestingly, co-treatment with DHA could effectively restore the anticancer effect of 5-FU against HCT116 TP53-/- cells, which manifested as the inhibition of proliferation and induction of reactive oxygen species (ROS)-mediated apoptosis and was accompanied by the upregulation of B-cell lymphoma 2 (BCL-2) and downregulation of the BCL-2-associated X protein (BAX). Fluorouracil 88-92 BCL2 apoptosis regulator Homo sapiens 284-301 29738772-5 2018 Interestingly, co-treatment with DHA could effectively restore the anticancer effect of 5-FU against HCT116 TP53-/- cells, which manifested as the inhibition of proliferation and induction of reactive oxygen species (ROS)-mediated apoptosis and was accompanied by the upregulation of B-cell lymphoma 2 (BCL-2) and downregulation of the BCL-2-associated X protein (BAX). Fluorouracil 88-92 BCL2 apoptosis regulator Homo sapiens 303-308 29620228-6 2018 Furthermore, the downregulation of beta-arrestin2 reduced the expression of the pro-apoptotic proteins cleaved-caspase-3 and Bax, and increased the expression of the anti-apoptotic protein Bcl-2 after 5-FU treatment. Fluorouracil 201-205 BCL2 apoptosis regulator Homo sapiens 189-194 29872316-7 2018 Apatinib combined with the aforementioned drugs, especially the combination of Apatinib and 5-FU, decreased the invasion and migration ability of the cells and increased the apoptosis ratio; expression of the anti-apoptotic protein Bcl-2 significantly decreased, and expression of the pro-apoptotic protein Bax increased. Fluorouracil 92-96 BCL2 apoptosis regulator Homo sapiens 232-237 29795190-4 2018 Enforced miR-181a expression enhanced 5-FU-induced p53-dependent mitochondrial apoptosis, including declined Bcl-2/Bax ratio, loss of mitochondrial membrane potential, cytochrome c release, and caspase-9 and caspase-3 activation. Fluorouracil 38-42 BCL2 apoptosis regulator Homo sapiens 109-114 29054700-8 2018 This is in contrast to 5-FU-induced suppression of Akt and mTOR activation, Bcl-2 and Bcl-xL expressions, and the enhanced expression of Bax and Bim. Fluorouracil 23-27 BCL2 apoptosis regulator Homo sapiens 76-81 29849804-8 2018 Additionally, bufalin combined with 5-FU reduced the expression levels of anti-apoptotic proteins, such as Mcl-1, XIAP and Bcl-2 and upregulated the levels of the pro-apoptotic proteins, Bax and Bad. Fluorouracil 36-40 BCL2 apoptosis regulator Homo sapiens 123-128 29636622-0 2018 miR-206 regulates 5-FU resistance by targeting Bcl-2 in colon cancer cells. Fluorouracil 18-22 BCL2 apoptosis regulator Homo sapiens 47-52 29636622-9 2018 We also identified miR-206 targeting Bcl-2 directly in CRC, which is required for miR-206 mediated-5-FU resistance. Fluorouracil 99-103 BCL2 apoptosis regulator Homo sapiens 37-42 28696828-6 2017 And our results reveal that chemotherapeutics, Cisplatin (CDDP) and 5-Fluoracil (5-FU), result in the greater decrease of protein level of Bcl-2 and Mcl-1 in NPC cells than those in NPE cells. Fluorouracil 68-79 BCL2 apoptosis regulator Homo sapiens 139-144 28531805-0 2017 Troxerutin (TXN) potentiated 5-Fluorouracil (5-Fu) treatment of human gastric cancer through suppressing STAT3/NF-kappaB and Bcl-2 signaling pathways. Fluorouracil 29-43 BCL2 apoptosis regulator Homo sapiens 125-130 28531805-0 2017 Troxerutin (TXN) potentiated 5-Fluorouracil (5-Fu) treatment of human gastric cancer through suppressing STAT3/NF-kappaB and Bcl-2 signaling pathways. Fluorouracil 45-49 BCL2 apoptosis regulator Homo sapiens 125-130 28531805-8 2017 Additionally, the presence of TXN sensitized gastric cancer cells resistant to 5-FU to 5-FU-induced apoptosis by suppressing Bcl-2. Fluorouracil 87-91 BCL2 apoptosis regulator Homo sapiens 125-130 28531805-11 2017 Our data indicated a novel therapeutic strategy to potentiate 5-FU-induced anti-tumor effect in gastric cancer cells with resistance to 5-FU by TXN through suppression of p-STAT3/NF-kappaB (p65 and p50) and Bcl-2. Fluorouracil 62-66 BCL2 apoptosis regulator Homo sapiens 207-212 28531805-11 2017 Our data indicated a novel therapeutic strategy to potentiate 5-FU-induced anti-tumor effect in gastric cancer cells with resistance to 5-FU by TXN through suppression of p-STAT3/NF-kappaB (p65 and p50) and Bcl-2. Fluorouracil 136-140 BCL2 apoptosis regulator Homo sapiens 207-212 28696828-6 2017 And our results reveal that chemotherapeutics, Cisplatin (CDDP) and 5-Fluoracil (5-FU), result in the greater decrease of protein level of Bcl-2 and Mcl-1 in NPC cells than those in NPE cells. Fluorouracil 81-85 BCL2 apoptosis regulator Homo sapiens 139-144 26392091-4 2016 The analysis of the changes in the expression of genes involved in the response to DNA damage (CDKN1A, GADD45A, MDM2), apoptosis (BAX, BCL2) and oncogenesis (MYC, JUN) showed that 5-FU, CDDP and ET upregulated the genes involved in DNA damage response, while the anti-apoptotic gene BCL2 and oncogene MYC were downregulated. Fluorouracil 180-184 BCL2 apoptosis regulator Homo sapiens 135-139 27330076-5 2016 Mechanistically, knockdown or inhibition of PDK4 significantly increases the inhibitory effect of 5-FU on expression of the anti-apoptotic factors Bcl-2 and survivin. Fluorouracil 98-102 BCL2 apoptosis regulator Homo sapiens 147-152 27924828-4 2016 Furthermore, we demonstrate that rpL3 significantly enhances the apoptosis of 5-FU treated Calu-6 cells promoting the overexpression of the pro-apoptotic proteins Bax and the inhibition of the anti-apoptotic protein Bcl-2. Fluorouracil 78-82 BCL2 apoptosis regulator Homo sapiens 216-221 27671231-5 2016 Co-treatment of DY and 5-FU significantly elevated ROS levels, up-regulated Bax/Bcl-2 ratio and triggered mitochondrial dysfunction, followed by a release of cytochrome c and up-regulation of proteins such as cleaved-caspase-9/3 and cleaved-PARP. Fluorouracil 23-27 BCL2 apoptosis regulator Homo sapiens 80-85 26392091-4 2016 The analysis of the changes in the expression of genes involved in the response to DNA damage (CDKN1A, GADD45A, MDM2), apoptosis (BAX, BCL2) and oncogenesis (MYC, JUN) showed that 5-FU, CDDP and ET upregulated the genes involved in DNA damage response, while the anti-apoptotic gene BCL2 and oncogene MYC were downregulated. Fluorouracil 180-184 BCL2 apoptosis regulator Homo sapiens 283-287 26051518-6 2015 Treatment with 5-Fluorouracil (5-FU), a frequently used but often clinically ineffective chemotherapy drug, induced apoptosis, down-regulation of anti-apoptotic genes (BCL-2, TRAF1, and c-FLIP) and decreased cell numbers in 2D, but only "nucleolar stress" in p3D and xenografts. Fluorouracil 15-29 BCL2 apoptosis regulator Homo sapiens 168-173 26785286-10 2016 CONCLUSION: These data reveal that 5-FU-induced cellular apoptosis in corneal epithelial cells may be mediated through caspase-8, caspase-9, and mitochondria-regulated pathways, as well as by upregulation of p21 and downregulation of Bcl-2-dependent signal transduction pathways. Fluorouracil 35-39 BCL2 apoptosis regulator Homo sapiens 234-239 26692822-13 2015 In addition, As2O3 in combination with 5-FU treatment caused up-regulation of DR5, caspase 3, caspase 8, and caspase 9, and down-regulation of BCL-2, but had no effect of DR4. Fluorouracil 39-43 BCL2 apoptosis regulator Homo sapiens 143-148 25736303-8 2015 The combination of 5-FU and Res significantly increased the percentage of apoptotic cells and the level of activated caspase-3, cleaved PARP and p53 proteins as well as increased the Bax/Bcl-2 ratio. Fluorouracil 19-23 BCL2 apoptosis regulator Homo sapiens 187-192 26892272-5 2016 Co-treatment with lupeol and 5-Fu induced apoptosis through up-regulating the expressions of Bax and p53 and down-regulating the expressions of survivin and Bcl-2. Fluorouracil 29-33 BCL2 apoptosis regulator Homo sapiens 157-162 26505786-6 2016 Treatment with 5-FU or DOX in combination with curcumin induced apoptosis by inhibiting Bcl-2 and increasing Bax, caspase-3, and poly-ADP ribose polymerase (PARP) in NT8e cells. Fluorouracil 15-19 BCL2 apoptosis regulator Homo sapiens 88-93 26136123-10 2015 We analyzed expression levels of survival and apoptosis-associated proteins (pAkt, Akt, Mcl-1, Bcl-2, Bad, and Bax) altered by 5-FU treatment. Fluorouracil 127-131 BCL2 apoptosis regulator Homo sapiens 95-100 26136123-11 2015 Survival and antiapoptosis signaling (pAkt, Akt, Mcl-1 and Bcl-2) was downregulated, and the proapoptotic proteins (Bad and Bax) were upregulated in 5-FU-treated control cells but expression levels of Bcl-2, Bad, and Bad were not altered in 5-FU-treated A7-nAChR-KD cells. Fluorouracil 149-153 BCL2 apoptosis regulator Homo sapiens 59-64 26136123-11 2015 Survival and antiapoptosis signaling (pAkt, Akt, Mcl-1 and Bcl-2) was downregulated, and the proapoptotic proteins (Bad and Bax) were upregulated in 5-FU-treated control cells but expression levels of Bcl-2, Bad, and Bad were not altered in 5-FU-treated A7-nAChR-KD cells. Fluorouracil 149-153 BCL2 apoptosis regulator Homo sapiens 201-206 26051518-6 2015 Treatment with 5-Fluorouracil (5-FU), a frequently used but often clinically ineffective chemotherapy drug, induced apoptosis, down-regulation of anti-apoptotic genes (BCL-2, TRAF1, and c-FLIP) and decreased cell numbers in 2D, but only "nucleolar stress" in p3D and xenografts. Fluorouracil 31-35 BCL2 apoptosis regulator Homo sapiens 168-173 24210071-6 2014 RESULTS: It was shown that in MCF-7 cells, the proliferation was inhibited, the apoptosis was promoted, the Bcl-2 expression was suppressed and the Bax expression was promoted by both 5-FU alone and morphine alone, while the superior effects were achieved in combination with the two drugs. Fluorouracil 184-188 BCL2 apoptosis regulator Homo sapiens 108-113 25323586-0 2015 Bcl-2 stabilization by paxillin confers 5-fluorouracil resistance in colorectal cancer. Fluorouracil 40-54 BCL2 apoptosis regulator Homo sapiens 0-5 25323586-4 2015 We thus hypothesized that pPXN-Y31/Y118 may be required for Bcl-2 protein stability via PXN interacting with Bcl-2 to confer 5-FU resistance in colorectal cancer. Fluorouracil 125-129 BCL2 apoptosis regulator Homo sapiens 60-65 25323586-4 2015 We thus hypothesized that pPXN-Y31/Y118 may be required for Bcl-2 protein stability via PXN interacting with Bcl-2 to confer 5-FU resistance in colorectal cancer. Fluorouracil 125-129 BCL2 apoptosis regulator Homo sapiens 109-114 25323586-7 2015 An increase in Bcl-2 expression by PXN is responsible for resistance to 5-FU. Fluorouracil 72-76 BCL2 apoptosis regulator Homo sapiens 15-20 25323586-8 2015 The resistance to 5-FU can be abolished by inhibitor of Src and PAK1 or Bcl-2 antagonist in cell and animal models. Fluorouracil 18-22 BCL2 apoptosis regulator Homo sapiens 72-77 25323586-10 2015 Patients with high PXN/high Bcl-2 or high pPXN-S272/high Bcl-2 tumors are commonly to have an unfavorable response to 5-FU-based chemotherapy, compared with patients who have high PXN, high pPXN-S272 or high Bcl-2 tumors alone. Fluorouracil 118-122 BCL2 apoptosis regulator Homo sapiens 28-33 25323586-10 2015 Patients with high PXN/high Bcl-2 or high pPXN-S272/high Bcl-2 tumors are commonly to have an unfavorable response to 5-FU-based chemotherapy, compared with patients who have high PXN, high pPXN-S272 or high Bcl-2 tumors alone. Fluorouracil 118-122 BCL2 apoptosis regulator Homo sapiens 57-62 25323586-10 2015 Patients with high PXN/high Bcl-2 or high pPXN-S272/high Bcl-2 tumors are commonly to have an unfavorable response to 5-FU-based chemotherapy, compared with patients who have high PXN, high pPXN-S272 or high Bcl-2 tumors alone. Fluorouracil 118-122 BCL2 apoptosis regulator Homo sapiens 57-62 25323586-11 2015 Therefore, we suggest that Src, PAK1 or Bcl-2 inhibitor may potentially overcome the resistance of 5-FU-based chemotherapy and consequently to improve outcomes in patients with PXN/Bcl-2 and pPXN-S272/Bcl-2-positive tumors. Fluorouracil 99-103 BCL2 apoptosis regulator Homo sapiens 40-45 25826088-1 2015 Stabilization of Bcl-2 protein by paxillin (PXN)-mediated ERK activation was recently reported to cause an unfavorable response to 5-Fluorouracil-based chemotherapy. Fluorouracil 131-145 BCL2 apoptosis regulator Homo sapiens 17-22 25348361-9 2015 In conclusion, DNA-PKcs suppression had complementary effects in combination with CDDP and 5-Fu treatment in HepG2 cells, which was associated with suppression of NF-kappaB signaling pathway cascade, activation of caspase-3 and p53, as well as down-regulation of Bcl-2 and GSH. Fluorouracil 91-95 BCL2 apoptosis regulator Homo sapiens 263-268 24751000-4 2014 Concentrations of 10, 50, and 100 ng/mL of 5-FU chemotherapeutic were added separately in Hep-2 cell line for 24 hours at 37 C. Cell sensibility was evaluated with fluorescein isothiocyanate (FITC) label Bcl-2 by flow cytometry. Fluorouracil 43-47 BCL2 apoptosis regulator Homo sapiens 205-210 24515389-5 2014 It"s found the inhibition rate of 5-FU, L-OHP to well-differentiated GC tissues and cell line was lower than that in the poorly differentiated tissues and cell line; expressions of ZNF139 and MDR1/P-gp, MRP1 and Bcl-2 in well-differentiated GC tissues and cell line MKN28 were higher, while Bax expression was lower. Fluorouracil 34-38 BCL2 apoptosis regulator Homo sapiens 212-217 24733045-5 2014 In further analysis, we show that JNK activation and phosphorylation of Bcl-2 are key determinants in 5-FU-induced autophagy. Fluorouracil 102-106 BCL2 apoptosis regulator Homo sapiens 72-77 24733045-7 2014 Taken together, our results suggest that JNK activation confers 5-FU resistance in HCT116 p53(-/-) and HT29 cells by promoting autophagy as a pro-survival effect, likely via inducing Bcl-2 phosphorylation. Fluorouracil 64-68 BCL2 apoptosis regulator Homo sapiens 183-188 25855960-7 2015 PCAF was also found to sensitize HCC cells to 5-fluorouracil (5-FU) treatment by regulating GLI1/Bcl-2/BAX axis-dependent apoptosis. Fluorouracil 46-60 BCL2 apoptosis regulator Homo sapiens 97-102 25855960-7 2015 PCAF was also found to sensitize HCC cells to 5-fluorouracil (5-FU) treatment by regulating GLI1/Bcl-2/BAX axis-dependent apoptosis. Fluorouracil 62-66 BCL2 apoptosis regulator Homo sapiens 97-102 25855960-10 2015 Together, these results show that PCAF can induce cell apoptosis by modulating a GLI1/Bcl-2/BAX axis that in turn suppresses HCC progression, and suggest that 5-FU may exert a stronger anti-tumor effect in patients with PCAF expression in HCC tumors. Fluorouracil 159-163 BCL2 apoptosis regulator Homo sapiens 86-91 26513239-11 2015 Overexpression of miR-429 inhibited Bcl-2-mediated cell survival against apoptosis induced by Fluorouracil, while depletion of miR-429 augmented it. Fluorouracil 94-106 BCL2 apoptosis regulator Homo sapiens 36-41 25230779-13 2014 Therefore, CD133 may inhibit 5-FU-induced apoptosis by regulating the expression of P-gp and Bcl-2 family mediated by phosphoinositide 3-kinase/Akt/p70S6K pathway in GC cells. Fluorouracil 29-33 BCL2 apoptosis regulator Homo sapiens 93-98 25019346-0 2014 Knockdown of EpCAM enhances the chemosensitivity of breast cancer cells to 5-fluorouracil by downregulating the antiapoptotic factor Bcl-2. Fluorouracil 75-89 BCL2 apoptosis regulator Homo sapiens 133-138 24161202-6 2013 CpG-ODN or 5-FU could down-regulate the mRNA expression of Bcl-2 within HepG2 cells. Fluorouracil 11-15 BCL2 apoptosis regulator Homo sapiens 59-64 24239278-3 2014 MATERIALS AND METHODS: The expression of EGFR, p53, Cyclin D1, p16, p21, p27, p-AKT, HIF-1alpha, Caspase 3 and BCL2 was analyzed by immunohistochemistry in 41 primary laryngeal/hypopharyngeal squamous cell carcinomas of patients that received induction chemotherapy (cisplatin and 5-fluorouracil) as part of their treatment. Fluorouracil 281-295 BCL2 apoptosis regulator Homo sapiens 111-115 24363520-10 2013 The up-regulation of Bax and down-regulation of Bcl-2 showed that the essential mechanism of apoptosis induced by SIN and 5-FU occurs via the mitochondrial pathway. Fluorouracil 122-126 BCL2 apoptosis regulator Homo sapiens 48-53 25087947-8 2014 Furthermore, combination treatment of GNA and 5-FU showed up-regulated of caspase-3, caspase-9, bax, RIP1, apoptosis-inducing factor (AIF), voltage-dependent anion channel (VDAC), cytochrome c and cyclophilin D and down-regulated bcl-2. Fluorouracil 46-50 BCL2 apoptosis regulator Homo sapiens 230-235 23684481-6 2013 Results showed that the siRNA could downregulate Bcl-2 expression, which increased apoptosis and sensitivity of SGC-7901 cell to 5-Fluorouracil (P<0.05). Fluorouracil 129-143 BCL2 apoptosis regulator Homo sapiens 49-54 24228120-7 2013 The positive rate of Bcl-2 expression was 80%, and Bcl-2 expression was significantly associated with chemoresistance to 5-FU (r(s) = 0.265, P = 0.041), ADM (r(s) = 0.425, P = 0.001) and MMC (r(s) = 0.40, P = 0.002). Fluorouracil 121-125 BCL2 apoptosis regulator Homo sapiens 51-56 24228120-9 2013 CONCLUSION: Overexpression of Bcl-2 may predict a loss of the efficacy of the chemotherapy drugs 5-FU, ADM and MMC in patients with gastric cancer. Fluorouracil 97-101 BCL2 apoptosis regulator Homo sapiens 30-35 23684481-0 2013 Bcl-2 gene silence enhances the sensitivity toward 5-Fluorouracil in gastric adenocarcinoma cells. Fluorouracil 51-65 BCL2 apoptosis regulator Homo sapiens 0-5 23684481-2 2013 This study was to investigate whether downregulation of Bcl-2 expression by small interfering RNA (siRNA) against the Bcl-2 gene would enhance the apoptosis and sensitivity of gastric adenocarcinoma SGC-7901 cell to 5-Fluorouracil. Fluorouracil 216-230 BCL2 apoptosis regulator Homo sapiens 56-61 23684481-7 2013 This study indicated that inhibition of Bcl-2 expression by siRNA would be useful a new useful protocol to increase the effect of 5-Fluorouracil on treatment of gastric adenocarcinoma, which may play an important role in developing novel therapeutic strategies in the future. Fluorouracil 130-144 BCL2 apoptosis regulator Homo sapiens 40-45 23684481-2 2013 This study was to investigate whether downregulation of Bcl-2 expression by small interfering RNA (siRNA) against the Bcl-2 gene would enhance the apoptosis and sensitivity of gastric adenocarcinoma SGC-7901 cell to 5-Fluorouracil. Fluorouracil 216-230 BCL2 apoptosis regulator Homo sapiens 118-123 23426065-6 2013 A drug resistance assay revealed that SP cells have a high survival rate when exposed to the chemotherapy drug 5-fluorouracil; the results of western blot analysis suggest that this stems from the abundant expression of the chemoresistance-associated proteins ABCG2 and Bcl-2. Fluorouracil 111-125 BCL2 apoptosis regulator Homo sapiens 270-275 23784204-4 2013 We demonstrated that the constructed Bcl-2 siRNA vector effectively silenced Bcl-2 gene expression in the GBC-SD human gallbladder carcinoma cells, inhibited cell proliferation, induced cell apoptosis, increased chemotherapeutic sensitivity to 5-fluorouracil and inhibited tumor growth in vivo. Fluorouracil 244-258 BCL2 apoptosis regulator Homo sapiens 37-42 23152059-8 2012 miR-204 targeted Bcl-2 messenger RNA and increased responsiveness of GC cells to 5-fluorouracil and oxaliplatin treatment. Fluorouracil 81-95 BCL2 apoptosis regulator Homo sapiens 17-22 23076534-6 2013 Periostin-overexpressing cells treated with cisplatin or 5-FU showed significantly (p < 0.05) decreased expression of Bax and p53 proteins and increased expression of Bcl-2 protein, when compared to drug-treated mock counterparts. Fluorouracil 57-61 BCL2 apoptosis regulator Homo sapiens 170-175 23152059-9 2012 Ectopic expression of Bcl-2 protein counteracted miR-204 pro-apoptotic activity in response to 5-fluorouracil. Fluorouracil 95-109 BCL2 apoptosis regulator Homo sapiens 22-27 22206670-5 2012 Moreover, we found that TQ enhanced the 5-FU-induced killing of gastric cancer cells by mediating the downregulation of the anti-apoptotic protein bcl-2, the upregulation of the pro-apoptotic protein bax, and the activation of both caspase-3 and caspase-9. Fluorouracil 40-44 BCL2 apoptosis regulator Homo sapiens 147-152 23108049-6 2012 5"-FU treatment dephosphorylated ERK in a DUSP6-dependent manner, resulting in destabilization of Bcl-2 and stabilization of Bad. Fluorouracil 0-5 BCL2 apoptosis regulator Homo sapiens 98-103 22825128-5 2012 The expression of apoptosis related proteins was also affected in cells treated with the combination of OA and 5-FU, including activation of caspases-3 and the expression of Bcl-2/Bax, survivin and NF-kappaB. Fluorouracil 123-127 BCL2 apoptosis regulator Homo sapiens 198-203 22607196-6 2012 The synergistic effect of 5-FU induced by oroxylin A was also found in the suppression of Bcl-2 and in the activation of P53, Bax, PARP, and procaspase-3 proteins in HT-29 cells. Fluorouracil 26-30 BCL2 apoptosis regulator Homo sapiens 90-95 22366766-0 2012 EBP50 gene transfection promotes 5-fluorouracil-induced apoptosis in gastric cancer cells through Bax- and Bcl-2-triggered mitochondrial pathways. Fluorouracil 33-47 BCL2 apoptosis regulator Homo sapiens 119-124