PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 21318867-5 2011 HuR is one of the best-studied RBPs and has emerged as a key regulator of posttranscriptional control of gene expression and its activity is tightly regulated by cellular polyamines. Polyamines 171-181 ELAV like RNA binding protein 1 Homo sapiens 0-3 27733365-5 2016 Increased levels of endogenous JunD by depleting cellular polyamines also inhibited importin-alpha1 expression and increased cytoplasmic HuR levels, whereas JunD silencing rescued importin-alpha1 expression and enhanced HuR nuclear translocation in polyamine-deficient cells. Polyamines 58-68 ELAV like RNA binding protein 1 Homo sapiens 137-140 27733365-5 2016 Increased levels of endogenous JunD by depleting cellular polyamines also inhibited importin-alpha1 expression and increased cytoplasmic HuR levels, whereas JunD silencing rescued importin-alpha1 expression and enhanced HuR nuclear translocation in polyamine-deficient cells. Polyamines 58-67 ELAV like RNA binding protein 1 Homo sapiens 137-140 24615811-0 2014 Stabilization of XIAP mRNA through the RNA binding protein HuR regulated by cellular polyamines. Polyamines 85-95 ELAV like RNA binding protein 1 Homo sapiens 59-62 20805360-0 2010 Polyamines regulate the stability of JunD mRNA by modulating the competitive binding of its 3" untranslated region to HuR and AUF1. Polyamines 0-10 ELAV like RNA binding protein 1 Homo sapiens 118-121 20805360-6 2010 The silencing of HuR increased AUF1 binding to the JunD mRNA, decreased the abundance of HuR-JunD mRNA complexes, rendered the JunD mRNA unstable, and prevented increases in JunD mRNA and protein in polyamine-deficient cells. Polyamines 199-208 ELAV like RNA binding protein 1 Homo sapiens 17-20 20805360-7 2010 Conversely, increasing the cellular polyamines repressed JunD mRNA interaction with HuR and enhanced its association with AUF1, resulting in an inhibition of JunD expression. Polyamines 36-46 ELAV like RNA binding protein 1 Homo sapiens 84-87 20805360-8 2010 These results indicate that polyamines modulate the stability of JunD mRNA in intestinal epithelial cells through HuR and AUF1 and provide new insight into the molecular functions of cellular polyamines. Polyamines 28-38 ELAV like RNA binding protein 1 Homo sapiens 114-117 20805360-5 2010 The depletion of cellular polyamines enhanced HuR binding to JunD mRNA and decreased the levels of JunD transcript associated with AUF1, thus stabilizing JunD mRNA. Polyamines 26-36 ELAV like RNA binding protein 1 Homo sapiens 46-49 20001965-6 2010 Inhibition of MEK-1 expression by MEK-1 silencing or HuR silencing prevented the increased resistance of polyamine-deficient cells to apoptosis. Polyamines 105-114 ELAV like RNA binding protein 1 Homo sapiens 53-56 20001965-0 2010 Post-transcriptional regulation of MEK-1 by polyamines through the RNA-binding protein HuR modulating intestinal epithelial apoptosis. Polyamines 44-54 ELAV like RNA binding protein 1 Homo sapiens 87-90 20001965-2 2010 In the present paper, we report that cellular polyamines regulate MEK-1 expression at the post-transcriptional level through the RNA-binding protein HuR (Hu-antigen R) in IECs (intestinal epithelial cells). Polyamines 46-56 ELAV like RNA binding protein 1 Homo sapiens 149-152 20001965-2 2010 In the present paper, we report that cellular polyamines regulate MEK-1 expression at the post-transcriptional level through the RNA-binding protein HuR (Hu-antigen R) in IECs (intestinal epithelial cells). Polyamines 46-56 ELAV like RNA binding protein 1 Homo sapiens 154-166 20001965-4 2010 HuR silencing rendered the MEK-1 mRNA unstable and inhibited its translation, thus preventing increases in MEK-1 mRNA and protein in polyamine-deficient cells. Polyamines 133-142 ELAV like RNA binding protein 1 Homo sapiens 0-3 20001965-8 2010 These results indicate that polyamines destabilize the MEK-1 mRNA and repress its translation by inhibiting the association between HuR and the MEK-1 transcript. Polyamines 28-38 ELAV like RNA binding protein 1 Homo sapiens 132-135 19812253-6 2009 Our findings demonstrate that polyamines regulate c-Myc translation in IECs through HuR phosphorylation by Chk2 and provide new insight into the molecular functions of cellular polyamines. Polyamines 30-40 ELAV like RNA binding protein 1 Homo sapiens 84-87 19812253-0 2009 Polyamines regulate c-Myc translation through Chk2-dependent HuR phosphorylation. Polyamines 0-10 ELAV like RNA binding protein 1 Homo sapiens 61-64 19812253-4 2009 Depletion of cellular polyamines inhibited Chk2 and reduced the affinity of HuR for c-Myc mRNA; these effects were completely reversed by addition of the polyamine putrescine or by Chk2 overexpression. Polyamines 22-32 ELAV like RNA binding protein 1 Homo sapiens 76-79 19812253-4 2009 Depletion of cellular polyamines inhibited Chk2 and reduced the affinity of HuR for c-Myc mRNA; these effects were completely reversed by addition of the polyamine putrescine or by Chk2 overexpression. Polyamines 22-31 ELAV like RNA binding protein 1 Homo sapiens 76-79 19825980-0 2009 Stabilization of XIAP mRNA through the RNA binding protein HuR regulated by cellular polyamines. Polyamines 85-95 ELAV like RNA binding protein 1 Homo sapiens 59-62 19825980-4 2009 Ectopic HuR overexpression or elevated cytoplasmic levels of endogenous HuR by decreasing cellular polyamines increased [HuR/XIAP mRNA] complexes, in turn promoting XIAP mRNA stability and increasing XIAP protein abundance. Polyamines 99-109 ELAV like RNA binding protein 1 Homo sapiens 8-11 19825980-4 2009 Ectopic HuR overexpression or elevated cytoplasmic levels of endogenous HuR by decreasing cellular polyamines increased [HuR/XIAP mRNA] complexes, in turn promoting XIAP mRNA stability and increasing XIAP protein abundance. Polyamines 99-109 ELAV like RNA binding protein 1 Homo sapiens 72-75 19825980-4 2009 Ectopic HuR overexpression or elevated cytoplasmic levels of endogenous HuR by decreasing cellular polyamines increased [HuR/XIAP mRNA] complexes, in turn promoting XIAP mRNA stability and increasing XIAP protein abundance. Polyamines 99-109 ELAV like RNA binding protein 1 Homo sapiens 72-75 19825980-5 2009 Conversely, HuR silencing in normal and polyamine-deficient cells rendered the XIAP mRNA unstable, thus reducing the steady state levels of XIAP. Polyamines 40-49 ELAV like RNA binding protein 1 Homo sapiens 12-15 19825980-6 2009 Inhibition of XIAP expression by XIAP silencing or by HuR silencing reversed the resistance of polyamine-deficient cells to apoptosis. Polyamines 95-104 ELAV like RNA binding protein 1 Homo sapiens 54-57 17919121-8 2008 These results indicate that polyamine-mediated AMPK activation triggers HuR nuclear import through phosphorylation and acetylation of Impalpha1 in IECs and that polyamine depletion increases cytoplasmic levels of HuR as a result of inactivation of the AMPK-driven Impalpha1 pathway. Polyamines 161-170 ELAV like RNA binding protein 1 Homo sapiens 213-216 17919121-0 2008 Polyamines modulate the subcellular localization of RNA-binding protein HuR through AMP-activated protein kinase-regulated phosphorylation and acetylation of importin alpha1. Polyamines 0-10 ELAV like RNA binding protein 1 Homo sapiens 72-75 17919121-1 2008 Polyamines are required for maintenance of intestinal epithelial integrity, and a decrease in cellular polyamines increases the cytoplasmic levels of RNA-binding protein HuR stabilizing p53 and nucleophosmin mRNAs, thus inhibiting IEC (intestinal epithelial cell) proliferation. Polyamines 103-113 ELAV like RNA binding protein 1 Homo sapiens 170-173 17919121-3 2008 Here, we provide evidence showing that polyamines modulate subcellular localization of HuR through AMPK-regulated phosphorylation and acetylation of Impalpha1 (importin alpha1) in IECs. Polyamines 39-49 ELAV like RNA binding protein 1 Homo sapiens 87-90 17919121-5 2008 AMPK activation by overexpression of the AMPK gene increased Impalpha1 but reduced the cytoplasmic levels of HuR in control and polyamine-deficient cells. Polyamines 128-137 ELAV like RNA binding protein 1 Homo sapiens 109-112 17919121-7 2008 Ectopic expression of these Impalpha1 mutants also prevented the increased levels of cytoplasmic HuR following polyamine depletion. Polyamines 111-120 ELAV like RNA binding protein 1 Homo sapiens 97-100 17919121-8 2008 These results indicate that polyamine-mediated AMPK activation triggers HuR nuclear import through phosphorylation and acetylation of Impalpha1 in IECs and that polyamine depletion increases cytoplasmic levels of HuR as a result of inactivation of the AMPK-driven Impalpha1 pathway. Polyamines 28-37 ELAV like RNA binding protein 1 Homo sapiens 72-75 17919121-8 2008 These results indicate that polyamine-mediated AMPK activation triggers HuR nuclear import through phosphorylation and acetylation of Impalpha1 in IECs and that polyamine depletion increases cytoplasmic levels of HuR as a result of inactivation of the AMPK-driven Impalpha1 pathway. Polyamines 28-37 ELAV like RNA binding protein 1 Homo sapiens 213-216 17804813-10 2007 These results indicate that polyamines modulate the stability of ATF-2 mRNA by altering cytoplasmic HuR levels and that polyamine-modulated ATF-2 expression plays a critical role in regulating epithelial apoptosis. Polyamines 28-38 ELAV like RNA binding protein 1 Homo sapiens 100-103 17804813-0 2007 Polyamines regulate the stability of activating transcription factor-2 mRNA through RNA-binding protein HuR in intestinal epithelial cells. Polyamines 0-10 ELAV like RNA binding protein 1 Homo sapiens 104-107 17804813-2 2007 Recently, polyamines were shown to regulate the subcellular localization of the RNA-binding protein HuR, which stabilizes its target transcripts such as nucleophosmin and p53 mRNAs. Polyamines 10-20 ELAV like RNA binding protein 1 Homo sapiens 100-103 17804813-7 2007 Increased cytoplasmic HuR in polyamine-deficient cells formed ribonucleoprotein complexes with the endogenous ATF-2 mRNA and specifically bound to 3"-UTR of ATF-2 mRNA on multiple nonoverlapping 3"-UTR segments. Polyamines 29-38 ELAV like RNA binding protein 1 Homo sapiens 22-25 17804813-10 2007 These results indicate that polyamines modulate the stability of ATF-2 mRNA by altering cytoplasmic HuR levels and that polyamine-modulated ATF-2 expression plays a critical role in regulating epithelial apoptosis. Polyamines 28-37 ELAV like RNA binding protein 1 Homo sapiens 100-103 16690610-0 2006 Polyamine depletion increases cytoplasmic levels of RNA-binding protein HuR leading to stabilization of nucleophosmin and p53 mRNAs. Polyamines 0-9 ELAV like RNA binding protein 1 Homo sapiens 72-75 16690610-4 2006 Depletion of cellular polyamines by inhibiting ornithine decarboxylase with alpha-difluoromethylornithine dramatically enhanced the cytoplasmic abundance of HuR, whereas ectopic ornithine decarboxylase overexpression decreased cytoplasmic HuR; neither intervention changed whole-cell HuR levels. Polyamines 22-32 ELAV like RNA binding protein 1 Homo sapiens 157-160 16690610-4 2006 Depletion of cellular polyamines by inhibiting ornithine decarboxylase with alpha-difluoromethylornithine dramatically enhanced the cytoplasmic abundance of HuR, whereas ectopic ornithine decarboxylase overexpression decreased cytoplasmic HuR; neither intervention changed whole-cell HuR levels. Polyamines 22-32 ELAV like RNA binding protein 1 Homo sapiens 239-242 16690610-4 2006 Depletion of cellular polyamines by inhibiting ornithine decarboxylase with alpha-difluoromethylornithine dramatically enhanced the cytoplasmic abundance of HuR, whereas ectopic ornithine decarboxylase overexpression decreased cytoplasmic HuR; neither intervention changed whole-cell HuR levels. Polyamines 22-32 ELAV like RNA binding protein 1 Homo sapiens 239-242 16690610-6 2006 HuR silencing rendered the NPM and p53 mRNAs unstable and prevented increases in NPM and p53 mRNA and protein in polyamine-deficient cells. Polyamines 113-122 ELAV like RNA binding protein 1 Homo sapiens 0-3 16690610-7 2006 These results indicate that polyamines modulate cytoplasmic HuR levels in intestinal epithelial cells, in turn controlling the stability of the NPM and p53 mRNAs and influencing NPM and p53 protein levels. Polyamines 28-38 ELAV like RNA binding protein 1 Homo sapiens 60-63