PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 7733268-0 1995 Orchidectomy and NMDA increase GnRH secretion as measured by push-pull perfusion of rat anterior pituitary. N-Methylaspartate 17-21 gonadotropin releasing hormone 1 Rattus norvegicus 31-35 7733268-4 1995 NMDA induced a rapid increase in plasma luteinizing hormone (LH) in both intact and castrated rats and increased GnRH concentrations in the perfusion samples (P < 0.05). N-Methylaspartate 0-4 gonadotropin releasing hormone 1 Rattus norvegicus 113-117 7733268-6 1995 These results demonstrate that GnRH release in the male rat is acutely increased by NMDA and is chronically increased after orchidectomy. N-Methylaspartate 84-88 gonadotropin releasing hormone 1 Rattus norvegicus 31-35 7602657-11 1995 In the second part of the experiment, the effect of pinealectomy on releasability of LHRH neurons was examined indirectly by NMDA-evoked LH secretion. N-Methylaspartate 125-129 gonadotropin releasing hormone 1 Rattus norvegicus 85-89 8119138-1 1994 Previous studies demonstrated that an excitatory amino acid analog, N-methyl-D-aspartate (NMDA), stimulates GnRH secretion in the rat, prepubertal primate, and ovine fetus at the hypothalamic level. N-Methylaspartate 68-88 gonadotropin releasing hormone 1 Rattus norvegicus 108-112 8062098-0 1994 Cross-talk between N-methyl-D-aspartate and adrenergic neurotransmission in the regulation of hypothalamic GnRH gene expression. N-Methylaspartate 19-39 gonadotropin releasing hormone 1 Rattus norvegicus 107-111 8062098-2 1994 It is then presumed that the action of NMDA on GnRH neurons may be indirectly mediated through interneurons, such as catecholamines. N-Methylaspartate 39-43 gonadotropin releasing hormone 1 Rattus norvegicus 47-51 8062098-3 1994 The present study is designed to investigate whether the effect of NMDA on GnRH gene expression is mediated by adrenergic neuronal system. N-Methylaspartate 67-71 gonadotropin releasing hormone 1 Rattus norvegicus 75-79 8062098-6 1994 Inhibition of either alpha 1 adrenergic receptor with prazosin or beta adrenergic receptor with propranolol did not cause any change in the basal GnRH mRNA levels but reduced NMDA-induced GnRH mRNA levels. N-Methylaspartate 175-179 gonadotropin releasing hormone 1 Rattus norvegicus 188-192 8062098-8 1994 These findings suggest that the effect of NMDA on GnRH gene expression is mediated through adrenergic neurotransmission. N-Methylaspartate 42-46 gonadotropin releasing hormone 1 Rattus norvegicus 50-54 8119138-1 1994 Previous studies demonstrated that an excitatory amino acid analog, N-methyl-D-aspartate (NMDA), stimulates GnRH secretion in the rat, prepubertal primate, and ovine fetus at the hypothalamic level. N-Methylaspartate 90-94 gonadotropin releasing hormone 1 Rattus norvegicus 108-112 8131083-5 1994 However, this substance clearly diminished LHRH release after stimulation with KCl (50 mM) or N-methyl-D-aspartate (10(-4) M). N-Methylaspartate 94-114 gonadotropin releasing hormone 1 Rattus norvegicus 43-47 8243268-13 1993 In addition, we tested the effects of NAME on NMDA-induced LHRH release in vitro from the median eminence-arcuate nucleus fragments. N-Methylaspartate 46-50 gonadotropin releasing hormone 1 Rattus norvegicus 59-63 8243268-15 1993 Addition of NO synthase inhibitor, NAME (1 or 10 mM) to suppress NO efflux, significantly diminished the NMDA-induced LHRH release. N-Methylaspartate 105-109 gonadotropin releasing hormone 1 Rattus norvegicus 118-122 8243268-14 1993 As expected, NMDA alone (50 mM) induced a significant increase in LHRH release. N-Methylaspartate 13-17 gonadotropin releasing hormone 1 Rattus norvegicus 66-70 8276955-7 1993 Activation of receptors to neuroexcitatory amino acids (N-methyl-D-aspartate; NMDA-type) was involved in the mechanism of pulsatile GnRH secretion. N-Methylaspartate 56-76 gonadotropin releasing hormone 1 Rattus norvegicus 132-136 8404677-1 1993 N-Methyl-D,L-aspartic acid (NMA), an agonist of N-methyl-D-aspartate (NMDA) excitatory amino acid receptors, stimulates the secretion of LH by increasing the release of LHRH. N-Methylaspartate 48-68 gonadotropin releasing hormone 1 Rattus norvegicus 169-173 7690119-9 1993 Basal LHRH release (OVX: 1.41 +/- 0.18; OVX-EB: 1.59 +/- 0.28 pg/10 min/MBH) was significantly (p < 0.05) enhanced by NMDA (OVX: 2.97 +/- 0.95; OVX-EB: 2.80 +/- 0.61 pg/10 min/MBH). N-Methylaspartate 121-125 gonadotropin releasing hormone 1 Rattus norvegicus 6-10 8319564-11 1993 The blockade of the inhibitory effects of the three peptides by NMDA and previous knowledge of hypothalamic sites of NMDA action suggest that EAA systems may represent a common pathway down-stream in the hypothalamic LHRH-regulating circuitry to mediate diminution of LH release by inhibitory peptides. N-Methylaspartate 117-121 gonadotropin releasing hormone 1 Rattus norvegicus 217-221 8276955-7 1993 Activation of receptors to neuroexcitatory amino acids (N-methyl-D-aspartate; NMDA-type) was involved in the mechanism of pulsatile GnRH secretion. N-Methylaspartate 78-82 gonadotropin releasing hormone 1 Rattus norvegicus 132-136 8381894-2 1993 NMDA induces significant increases in plasma LH in a variety of animal models and these effects occur by activation of neural processes involved in excitation of LHRH neurons rather than by a direct action on the pituitary gland. N-Methylaspartate 0-4 gonadotropin releasing hormone 1 Rattus norvegicus 162-166 8381894-7 1993 Within 1 h after NMDA, LHRH mRNA had increased significantly in OVLT and rPOA but not in mPOA and cPOA neurons of control rats and these mRNA levels remained elevated for 4 h. In contrast, NMDA treatment of ASR did not affect basal levels of LHRH mRNA in any region of the rostral hypothalamus. N-Methylaspartate 17-21 gonadotropin releasing hormone 1 Rattus norvegicus 23-27 8381894-7 1993 Within 1 h after NMDA, LHRH mRNA had increased significantly in OVLT and rPOA but not in mPOA and cPOA neurons of control rats and these mRNA levels remained elevated for 4 h. In contrast, NMDA treatment of ASR did not affect basal levels of LHRH mRNA in any region of the rostral hypothalamus. N-Methylaspartate 189-193 gonadotropin releasing hormone 1 Rattus norvegicus 242-246 8381894-8 1993 These observations suggest that neonatal androgen treatment of female rats either directly or indirectly affects the responsiveness of LHRH neurons to NMDA. N-Methylaspartate 151-155 gonadotropin releasing hormone 1 Rattus norvegicus 135-139 1874197-0 1991 Rapid increase in LHRH mRNA levels following NMDA. N-Methylaspartate 45-49 gonadotropin releasing hormone 1 Rattus norvegicus 18-22 1347007-3 1992 The increase of LH levels induced by NMDA was accompanied by a significant enhancement of the content of GnRH in the anterior and preoptic hypothalamic areas and in the medial basal hypothalamus (APOA/MBH). N-Methylaspartate 37-41 gonadotropin releasing hormone 1 Rattus norvegicus 105-109 1347007-4 1992 EP potentiated this increase of GnRH induced by NMDA. N-Methylaspartate 48-52 gonadotropin releasing hormone 1 Rattus norvegicus 32-36 1425026-2 1992 The interaction is amongst noradrenaline and other neurotransmitters such as GABA (gamma-aminobutyric acid), opiates, serotonin and excitatory amino acids (N-methyl-D-aspartate, NMDA) on LHRH neuronal activity are complex. N-Methylaspartate 156-176 gonadotropin releasing hormone 1 Rattus norvegicus 187-191 1425026-9 1992 NMDA rapidly releases LH and LHRH mRNA levels are significantly raised within 15 minutes and remain so over the next 45 minutes. N-Methylaspartate 0-4 gonadotropin releasing hormone 1 Rattus norvegicus 29-33 1874197-1 1991 To test the hypothesis that changes in LHRH mRNA levels are tightly linked to changes in LHRH secretion, intact male rats were infused with NMDA, a substance which increases LH release by a hypothalamic action. N-Methylaspartate 140-144 gonadotropin releasing hormone 1 Rattus norvegicus 39-43 1874197-1 1991 To test the hypothesis that changes in LHRH mRNA levels are tightly linked to changes in LHRH secretion, intact male rats were infused with NMDA, a substance which increases LH release by a hypothalamic action. N-Methylaspartate 140-144 gonadotropin releasing hormone 1 Rattus norvegicus 89-93 1874197-2 1991 NMDA significantly elevated cellular levels of LHRH mRNA at 15 and 60 min. N-Methylaspartate 0-4 gonadotropin releasing hormone 1 Rattus norvegicus 47-51 1874197-4 1991 These effects of NMDA on LHRH mRNA levels were not due to a generalized excitatory effect in the brain because levels of mRNAs encoding proopiomelanocortin (POMC) and tyrosine hydroxylase (TH) in the arcuate-periarcuate region were not altered by NMDA and levels of TH mRNA were decreased significantly at 15 and 60 min in neurons of the zona incerta. N-Methylaspartate 17-21 gonadotropin releasing hormone 1 Rattus norvegicus 25-29 19390953-1 2009 In previous studies we have found that blockade of NMDA (N-Methyl-D-Aspartic-Acid)-type glutamatergic receptor with intracerebroventricular (ICV) selective drugs induces an inhibition of lordosis in ovariectomized (OVX) estrogen primed rats receiving progesterone or luteinizing hormone releasing hormone (LHRH). N-Methylaspartate 51-55 gonadotropin releasing hormone 1 Rattus norvegicus 267-304 1900231-8 1991 The gonadotropin-releasing effect of NMDA may be mediated at the level of the hypothalamus, since the medial basal hypothalamus/preoptic area of NMDA-treated rats killed 3 and 5 min post-NMDA had a significantly greater release of GnRH in vitro than that of vehicle-treated rats. N-Methylaspartate 37-41 gonadotropin releasing hormone 1 Rattus norvegicus 231-235 1900231-8 1991 The gonadotropin-releasing effect of NMDA may be mediated at the level of the hypothalamus, since the medial basal hypothalamus/preoptic area of NMDA-treated rats killed 3 and 5 min post-NMDA had a significantly greater release of GnRH in vitro than that of vehicle-treated rats. N-Methylaspartate 145-149 gonadotropin releasing hormone 1 Rattus norvegicus 231-235 1900231-8 1991 The gonadotropin-releasing effect of NMDA may be mediated at the level of the hypothalamus, since the medial basal hypothalamus/preoptic area of NMDA-treated rats killed 3 and 5 min post-NMDA had a significantly greater release of GnRH in vitro than that of vehicle-treated rats. N-Methylaspartate 145-149 gonadotropin releasing hormone 1 Rattus norvegicus 231-235 2164923-7 1990 Such a concept was supported by the NMDA-induced release of GnRH, which was observed using 1 mM NMDA at 25 days, while a dose of 20-50 mM was required at 15 or 50 days of age. N-Methylaspartate 36-40 gonadotropin releasing hormone 1 Rattus norvegicus 60-64 2164923-7 1990 Such a concept was supported by the NMDA-induced release of GnRH, which was observed using 1 mM NMDA at 25 days, while a dose of 20-50 mM was required at 15 or 50 days of age. N-Methylaspartate 96-100 gonadotropin releasing hormone 1 Rattus norvegicus 60-64 2541362-2 1989 The in vitro release of GnRH from the hypothalamus showed a maximal increase (4.6-fold) in animals sacrificed 7.5 min after NMDA injection, while serum LH levels rose concomitantly. N-Methylaspartate 124-128 gonadotropin releasing hormone 1 Rattus norvegicus 24-28 2541362-3 1989 Incubation of rat hypothalami in vitro with kainate or NMDA concentrations greater than 0.1 mM resulted in a dose-related release of GnRH, NMDA being twofold more potent than kainate. N-Methylaspartate 55-59 gonadotropin releasing hormone 1 Rattus norvegicus 133-137 2541362-3 1989 Incubation of rat hypothalami in vitro with kainate or NMDA concentrations greater than 0.1 mM resulted in a dose-related release of GnRH, NMDA being twofold more potent than kainate. N-Methylaspartate 139-143 gonadotropin releasing hormone 1 Rattus norvegicus 133-137 2541362-6 1989 The effects of kainate and NMDA were mediated through different types of receptors, since GnRH response to kainate was unchanged in the absence of glycine or in the presence of increased concentrations of Mg2+ (2 mM) or Ca2+ (5.8 mM). N-Methylaspartate 27-31 gonadotropin releasing hormone 1 Rattus norvegicus 90-94 2541362-7 1989 In contrast, the GnRH response to NMDA was reduced by Ca2+ (5.8 mM) and abolished in the absence of glycine or in the presence of Mg2+ (2 mM). N-Methylaspartate 34-38 gonadotropin releasing hormone 1 Rattus norvegicus 17-21 2541362-8 1989 In addition, D,L-amino-5-phosphonopentanoic acid (AP5), a competitive antagonist of NMDA receptors, prevented the NMDA-induced release of GnRH. N-Methylaspartate 84-88 gonadotropin releasing hormone 1 Rattus norvegicus 138-142 2541362-9 1989 The permissive effect of glycine on GnRH response to NMDA was 2.7-fold more important using glycine concentrations of 0.01 microM than when concentrations greater than or equal to 100 microM were used. N-Methylaspartate 53-57 gonadotropin releasing hormone 1 Rattus norvegicus 36-40 3283488-2 1988 The present studies were carried out to determine whether the effects of NMDA on LH were mediated by the release of LHRH from the hypothalamus. N-Methylaspartate 73-77 gonadotropin releasing hormone 1 Rattus norvegicus 116-120 1958516-7 1991 When tested in a perifusion system on hypothalamic and anterior pituitary tissues, they differentially stimulate the release of LHRH (NMDA) and of LH (KA); their effect on both structures is markedly reduced following orchidectomy. N-Methylaspartate 134-138 gonadotropin releasing hormone 1 Rattus norvegicus 128-132 2123444-11 1990 During exposure to GnRH-A, the NMDA-induced release of GnRH was only reduced by 38% whereas veratridine-induced secretion showed a 94% reduction. N-Methylaspartate 31-35 gonadotropin releasing hormone 1 Rattus norvegicus 19-25 2123444-11 1990 During exposure to GnRH-A, the NMDA-induced release of GnRH was only reduced by 38% whereas veratridine-induced secretion showed a 94% reduction. N-Methylaspartate 31-35 gonadotropin releasing hormone 1 Rattus norvegicus 19-23 2546737-1 1989 We have shown previously that N-methyl-D,L-aspartate (NMDA) and kainate, two neuroexcitatory amino acids acting through distinct receptors, may induce the release of GnRH from hypothalamic explants. N-Methylaspartate 54-58 gonadotropin releasing hormone 1 Rattus norvegicus 166-170 2546737-6 1989 The stimulation of GnRH release by NMDA (50 mM) added to the medium for 7.5 min could be blocked reversibly in the presence of MK-801 (100 microM) using medium without glycine or enriched with Mg2+ (2 mM). N-Methylaspartate 35-39 gonadotropin releasing hormone 1 Rattus norvegicus 19-23 2546737-8 1989 In contrast, pulsatile release of GnRH was not affected in the presence of 6,7-dinitroquinoxaline-2,3-dione (0.1 mM), a selective inhibitor of kainate and quisqualate receptors which suppressed the increase in GnRH release induced by kainate (50 mM) without affecting the response to NMDA. N-Methylaspartate 284-288 gonadotropin releasing hormone 1 Rattus norvegicus 34-38 3283488-6 1988 Our studies also demonstrated that NMDA increased LH via a direct effect on the hypothalamic release of LHRH since a potent LHRH antagonist competitively inhibited the effects of NMDA. N-Methylaspartate 35-39 gonadotropin releasing hormone 1 Rattus norvegicus 104-108 3283488-6 1988 Our studies also demonstrated that NMDA increased LH via a direct effect on the hypothalamic release of LHRH since a potent LHRH antagonist competitively inhibited the effects of NMDA. N-Methylaspartate 35-39 gonadotropin releasing hormone 1 Rattus norvegicus 124-128 3283488-6 1988 Our studies also demonstrated that NMDA increased LH via a direct effect on the hypothalamic release of LHRH since a potent LHRH antagonist competitively inhibited the effects of NMDA. N-Methylaspartate 179-183 gonadotropin releasing hormone 1 Rattus norvegicus 104-108 3283488-6 1988 Our studies also demonstrated that NMDA increased LH via a direct effect on the hypothalamic release of LHRH since a potent LHRH antagonist competitively inhibited the effects of NMDA. N-Methylaspartate 179-183 gonadotropin releasing hormone 1 Rattus norvegicus 124-128 30916745-3 2019 N-methyl-D-aspartate (NMDA) can promote pulsatile GnRH secretion and accelerates puberty onset. N-Methylaspartate 0-20 gonadotropin releasing hormone 1 Rattus norvegicus 50-54 30916745-3 2019 N-methyl-D-aspartate (NMDA) can promote pulsatile GnRH secretion and accelerates puberty onset. N-Methylaspartate 22-26 gonadotropin releasing hormone 1 Rattus norvegicus 50-54 18096065-3 2007 Moreover, endogenous NMDA has been found in rat, where it has a role in the induction of GnRH (Gonadotropin Releasing Hormone) in the hypothalamus, and of LH (Luteinizing Hormone) and PRL (Prolactin) in the pituitary gland. N-Methylaspartate 21-25 gonadotropin releasing hormone 1 Rattus norvegicus 89-93 18096065-3 2007 Moreover, endogenous NMDA has been found in rat, where it has a role in the induction of GnRH (Gonadotropin Releasing Hormone) in the hypothalamus, and of LH (Luteinizing Hormone) and PRL (Prolactin) in the pituitary gland. N-Methylaspartate 21-25 gonadotropin releasing hormone 1 Rattus norvegicus 95-125 16388117-2 2005 Glutamate, N-methyl-D-aspartate (NMDA), and kainate stimulate gonadotropin-releasing hormone (GnRH) secretion in immature mammals and NMDA receptor stimulation results in precocious puberty in rats and monkeys. N-Methylaspartate 11-31 gonadotropin releasing hormone 1 Rattus norvegicus 62-92 16501816-1 2006 We have observed that intracerebroventricular (icv) injection of selective N-methyl-D-aspartic acid (NMDA)-type glutamatergic receptor antagonists inhibits lordosis in ovariectomized (OVX), estrogen-primed rats receiving progesterone or luteinizing hormone-releasing hormone (LHRH). N-Methylaspartate 75-99 gonadotropin releasing hormone 1 Rattus norvegicus 237-274 16501816-1 2006 We have observed that intracerebroventricular (icv) injection of selective N-methyl-D-aspartic acid (NMDA)-type glutamatergic receptor antagonists inhibits lordosis in ovariectomized (OVX), estrogen-primed rats receiving progesterone or luteinizing hormone-releasing hormone (LHRH). N-Methylaspartate 75-99 gonadotropin releasing hormone 1 Rattus norvegicus 276-280 16501816-1 2006 We have observed that intracerebroventricular (icv) injection of selective N-methyl-D-aspartic acid (NMDA)-type glutamatergic receptor antagonists inhibits lordosis in ovariectomized (OVX), estrogen-primed rats receiving progesterone or luteinizing hormone-releasing hormone (LHRH). N-Methylaspartate 101-105 gonadotropin releasing hormone 1 Rattus norvegicus 237-274 16501816-1 2006 We have observed that intracerebroventricular (icv) injection of selective N-methyl-D-aspartic acid (NMDA)-type glutamatergic receptor antagonists inhibits lordosis in ovariectomized (OVX), estrogen-primed rats receiving progesterone or luteinizing hormone-releasing hormone (LHRH). N-Methylaspartate 101-105 gonadotropin releasing hormone 1 Rattus norvegicus 276-280 16388117-2 2005 Glutamate, N-methyl-D-aspartate (NMDA), and kainate stimulate gonadotropin-releasing hormone (GnRH) secretion in immature mammals and NMDA receptor stimulation results in precocious puberty in rats and monkeys. N-Methylaspartate 11-31 gonadotropin releasing hormone 1 Rattus norvegicus 94-98 16388117-2 2005 Glutamate, N-methyl-D-aspartate (NMDA), and kainate stimulate gonadotropin-releasing hormone (GnRH) secretion in immature mammals and NMDA receptor stimulation results in precocious puberty in rats and monkeys. N-Methylaspartate 33-37 gonadotropin releasing hormone 1 Rattus norvegicus 62-92 16388117-2 2005 Glutamate, N-methyl-D-aspartate (NMDA), and kainate stimulate gonadotropin-releasing hormone (GnRH) secretion in immature mammals and NMDA receptor stimulation results in precocious puberty in rats and monkeys. N-Methylaspartate 33-37 gonadotropin releasing hormone 1 Rattus norvegicus 94-98 8757241-6 1996 The acceleration of the elevation in GnRH mRNA levels by NMDA suggests that a stimulation of GnRH gene expression may be a rate-limiting factor for the onset of puberty. N-Methylaspartate 57-61 gonadotropin releasing hormone 1 Rattus norvegicus 37-41 12876296-8 2003 E + N-methyl-D-aspartic acid (NMDA), an excitatory amino acid for 30 min to study the effect of prior and continued exposure to vitamin E on NMDA-induced LHRH release. N-Methylaspartate 141-145 gonadotropin releasing hormone 1 Rattus norvegicus 154-158 12876296-12 2003 NMDA stimulated LHRH release as previously shown and this effect was not altered in the combined presence of vitamin E plus NMDA. N-Methylaspartate 0-4 gonadotropin releasing hormone 1 Rattus norvegicus 16-20 11012845-5 2000 Treatment with the glutamate agonist NMDA caused a significant increase in GnRH mRNA levels in neonatal (P0) mice and adult male mice within 30 min of treatment, which is consistent with previous studies in the rat implicating glutamate in the regulation of GnRH mRNA stability. N-Methylaspartate 37-41 gonadotropin releasing hormone 1 Rattus norvegicus 258-262 9387849-4 1997 GnRH is released from the three different types of explant in response to depolarization with veratridine or glutamate receptor stimulation using the agonist N-methyl-D-aspartate. N-Methylaspartate 158-178 gonadotropin releasing hormone 1 Rattus norvegicus 0-4 8973819-0 1996 Effects of aging on N-methyl-D-aspartate (NMDA)-induced GnRH and LH release in female rats. N-Methylaspartate 20-40 gonadotropin releasing hormone 1 Rattus norvegicus 56-60 8973819-0 1996 Effects of aging on N-methyl-D-aspartate (NMDA)-induced GnRH and LH release in female rats. N-Methylaspartate 42-46 gonadotropin releasing hormone 1 Rattus norvegicus 56-60 8973819-4 1996 The addition of NMDA to the medium significantly increased GnRH release in both groups; nevertheless, the GnRH release response to NMDA was significantly lower in old (P < 0.01) than in young rats (Young: Basal: 50 +/- 10; NMDA 15": 410 +/- 63, 22,5": 1,469 +/- 300; Old: Basal: 47 +/- 10; NMDA 15": 210 +/- 30; 22,5": 350 +/- 65 ng/GnRH/mg.protein). N-Methylaspartate 16-20 gonadotropin releasing hormone 1 Rattus norvegicus 59-63 8973819-4 1996 The addition of NMDA to the medium significantly increased GnRH release in both groups; nevertheless, the GnRH release response to NMDA was significantly lower in old (P < 0.01) than in young rats (Young: Basal: 50 +/- 10; NMDA 15": 410 +/- 63, 22,5": 1,469 +/- 300; Old: Basal: 47 +/- 10; NMDA 15": 210 +/- 30; 22,5": 350 +/- 65 ng/GnRH/mg.protein). N-Methylaspartate 16-20 gonadotropin releasing hormone 1 Rattus norvegicus 106-110 8973819-4 1996 The addition of NMDA to the medium significantly increased GnRH release in both groups; nevertheless, the GnRH release response to NMDA was significantly lower in old (P < 0.01) than in young rats (Young: Basal: 50 +/- 10; NMDA 15": 410 +/- 63, 22,5": 1,469 +/- 300; Old: Basal: 47 +/- 10; NMDA 15": 210 +/- 30; 22,5": 350 +/- 65 ng/GnRH/mg.protein). N-Methylaspartate 16-20 gonadotropin releasing hormone 1 Rattus norvegicus 106-110 8973819-4 1996 The addition of NMDA to the medium significantly increased GnRH release in both groups; nevertheless, the GnRH release response to NMDA was significantly lower in old (P < 0.01) than in young rats (Young: Basal: 50 +/- 10; NMDA 15": 410 +/- 63, 22,5": 1,469 +/- 300; Old: Basal: 47 +/- 10; NMDA 15": 210 +/- 30; 22,5": 350 +/- 65 ng/GnRH/mg.protein). N-Methylaspartate 131-135 gonadotropin releasing hormone 1 Rattus norvegicus 106-110 8973819-4 1996 The addition of NMDA to the medium significantly increased GnRH release in both groups; nevertheless, the GnRH release response to NMDA was significantly lower in old (P < 0.01) than in young rats (Young: Basal: 50 +/- 10; NMDA 15": 410 +/- 63, 22,5": 1,469 +/- 300; Old: Basal: 47 +/- 10; NMDA 15": 210 +/- 30; 22,5": 350 +/- 65 ng/GnRH/mg.protein). N-Methylaspartate 131-135 gonadotropin releasing hormone 1 Rattus norvegicus 106-110 8973819-4 1996 The addition of NMDA to the medium significantly increased GnRH release in both groups; nevertheless, the GnRH release response to NMDA was significantly lower in old (P < 0.01) than in young rats (Young: Basal: 50 +/- 10; NMDA 15": 410 +/- 63, 22,5": 1,469 +/- 300; Old: Basal: 47 +/- 10; NMDA 15": 210 +/- 30; 22,5": 350 +/- 65 ng/GnRH/mg.protein). N-Methylaspartate 131-135 gonadotropin releasing hormone 1 Rattus norvegicus 106-110 8973819-4 1996 The addition of NMDA to the medium significantly increased GnRH release in both groups; nevertheless, the GnRH release response to NMDA was significantly lower in old (P < 0.01) than in young rats (Young: Basal: 50 +/- 10; NMDA 15": 410 +/- 63, 22,5": 1,469 +/- 300; Old: Basal: 47 +/- 10; NMDA 15": 210 +/- 30; 22,5": 350 +/- 65 ng/GnRH/mg.protein). N-Methylaspartate 131-135 gonadotropin releasing hormone 1 Rattus norvegicus 106-110 8973819-4 1996 The addition of NMDA to the medium significantly increased GnRH release in both groups; nevertheless, the GnRH release response to NMDA was significantly lower in old (P < 0.01) than in young rats (Young: Basal: 50 +/- 10; NMDA 15": 410 +/- 63, 22,5": 1,469 +/- 300; Old: Basal: 47 +/- 10; NMDA 15": 210 +/- 30; 22,5": 350 +/- 65 ng/GnRH/mg.protein). N-Methylaspartate 131-135 gonadotropin releasing hormone 1 Rattus norvegicus 106-110 8973819-4 1996 The addition of NMDA to the medium significantly increased GnRH release in both groups; nevertheless, the GnRH release response to NMDA was significantly lower in old (P < 0.01) than in young rats (Young: Basal: 50 +/- 10; NMDA 15": 410 +/- 63, 22,5": 1,469 +/- 300; Old: Basal: 47 +/- 10; NMDA 15": 210 +/- 30; 22,5": 350 +/- 65 ng/GnRH/mg.protein). N-Methylaspartate 131-135 gonadotropin releasing hormone 1 Rattus norvegicus 106-110 8973819-9 1996 In conclusion, present results demonstrate that the GnRH responses to NMDA neurotransmission, which has a predominantly excitatory effects on GnRH neurons, is significantly decreased in old rats, these data give further support to the hypothesis that a decrease in the excitatory inputs to GnRH neurons could be directly involved in the reduction of the hypothalamic-pituitary-ovary axis activity observed during aging. N-Methylaspartate 70-74 gonadotropin releasing hormone 1 Rattus norvegicus 52-56 8973819-9 1996 In conclusion, present results demonstrate that the GnRH responses to NMDA neurotransmission, which has a predominantly excitatory effects on GnRH neurons, is significantly decreased in old rats, these data give further support to the hypothesis that a decrease in the excitatory inputs to GnRH neurons could be directly involved in the reduction of the hypothalamic-pituitary-ovary axis activity observed during aging. N-Methylaspartate 70-74 gonadotropin releasing hormone 1 Rattus norvegicus 142-146 8973819-9 1996 In conclusion, present results demonstrate that the GnRH responses to NMDA neurotransmission, which has a predominantly excitatory effects on GnRH neurons, is significantly decreased in old rats, these data give further support to the hypothesis that a decrease in the excitatory inputs to GnRH neurons could be directly involved in the reduction of the hypothalamic-pituitary-ovary axis activity observed during aging. N-Methylaspartate 70-74 gonadotropin releasing hormone 1 Rattus norvegicus 142-146 15385417-3 2005 GnRH secretory response to repeated N-methyl-D-aspartate (NMDA) stimulation showed progressive disappearance, indicating that the inhibitory autofeedback was operating. N-Methylaspartate 36-56 gonadotropin releasing hormone 1 Rattus norvegicus 0-4 15385417-3 2005 GnRH secretory response to repeated N-methyl-D-aspartate (NMDA) stimulation showed progressive disappearance, indicating that the inhibitory autofeedback was operating. N-Methylaspartate 58-62 gonadotropin releasing hormone 1 Rattus norvegicus 0-4 10601818-0 2000 Interleukin-1 inhibits NMDA-stimulated GnRH secretion: associated effects on the release of hypothalamic inhibitory amino acid neurotransmitters. N-Methylaspartate 23-27 gonadotropin releasing hormone 1 Rattus norvegicus 39-43 10601818-4 2000 5 and 5 nM) on basal and N-methyl-D-aspartate (NMDA)-stimulated release of gonadotropin-releasing hormone (GnRH), and the associated modifications in the output of inhibitory amino acid neurotransmitters involved in the control of GnRH secretion. N-Methylaspartate 25-45 gonadotropin releasing hormone 1 Rattus norvegicus 75-105 10601818-4 2000 5 and 5 nM) on basal and N-methyl-D-aspartate (NMDA)-stimulated release of gonadotropin-releasing hormone (GnRH), and the associated modifications in the output of inhibitory amino acid neurotransmitters involved in the control of GnRH secretion. N-Methylaspartate 25-45 gonadotropin releasing hormone 1 Rattus norvegicus 107-111 10601818-4 2000 5 and 5 nM) on basal and N-methyl-D-aspartate (NMDA)-stimulated release of gonadotropin-releasing hormone (GnRH), and the associated modifications in the output of inhibitory amino acid neurotransmitters involved in the control of GnRH secretion. N-Methylaspartate 25-45 gonadotropin releasing hormone 1 Rattus norvegicus 231-235 10601818-4 2000 5 and 5 nM) on basal and N-methyl-D-aspartate (NMDA)-stimulated release of gonadotropin-releasing hormone (GnRH), and the associated modifications in the output of inhibitory amino acid neurotransmitters involved in the control of GnRH secretion. N-Methylaspartate 47-51 gonadotropin releasing hormone 1 Rattus norvegicus 75-105 10601818-4 2000 5 and 5 nM) on basal and N-methyl-D-aspartate (NMDA)-stimulated release of gonadotropin-releasing hormone (GnRH), and the associated modifications in the output of inhibitory amino acid neurotransmitters involved in the control of GnRH secretion. N-Methylaspartate 47-51 gonadotropin releasing hormone 1 Rattus norvegicus 107-111 10601818-4 2000 5 and 5 nM) on basal and N-methyl-D-aspartate (NMDA)-stimulated release of gonadotropin-releasing hormone (GnRH), and the associated modifications in the output of inhibitory amino acid neurotransmitters involved in the control of GnRH secretion. N-Methylaspartate 47-51 gonadotropin releasing hormone 1 Rattus norvegicus 231-235 10601818-5 2000 IL-1 did not modify basal GnRH release, but markedly restrained the stimulatory effect of NMDA on GnRH secretion. N-Methylaspartate 90-94 gonadotropin releasing hormone 1 Rattus norvegicus 98-102 10601818-7 2000 Our results indicate that this cytokine inhibits NMDA- stimulated GnRH release, affecting the activity and/or the release of hypothalamic excitatory and inhibitory amino acid neurotransmitters participating in the regulation of GnRH secretion. N-Methylaspartate 49-53 gonadotropin releasing hormone 1 Rattus norvegicus 66-70 10601818-7 2000 Our results indicate that this cytokine inhibits NMDA- stimulated GnRH release, affecting the activity and/or the release of hypothalamic excitatory and inhibitory amino acid neurotransmitters participating in the regulation of GnRH secretion. N-Methylaspartate 49-53 gonadotropin releasing hormone 1 Rattus norvegicus 228-232 10575094-1 1999 We showed previously that insulin-like growth factor-I (IGF-I) could inhibit the secretion of gonadotrophin-releasing hormone (GnRH) evoked in vitro by N-methyl-D-aspartate (NMDA) or veratridine depolarization. N-Methylaspartate 152-172 gonadotropin releasing hormone 1 Rattus norvegicus 94-125 10575094-1 1999 We showed previously that insulin-like growth factor-I (IGF-I) could inhibit the secretion of gonadotrophin-releasing hormone (GnRH) evoked in vitro by N-methyl-D-aspartate (NMDA) or veratridine depolarization. N-Methylaspartate 152-172 gonadotropin releasing hormone 1 Rattus norvegicus 127-131 10575094-1 1999 We showed previously that insulin-like growth factor-I (IGF-I) could inhibit the secretion of gonadotrophin-releasing hormone (GnRH) evoked in vitro by N-methyl-D-aspartate (NMDA) or veratridine depolarization. N-Methylaspartate 174-178 gonadotropin releasing hormone 1 Rattus norvegicus 94-125 10575094-1 1999 We showed previously that insulin-like growth factor-I (IGF-I) could inhibit the secretion of gonadotrophin-releasing hormone (GnRH) evoked in vitro by N-methyl-D-aspartate (NMDA) or veratridine depolarization. N-Methylaspartate 174-178 gonadotropin releasing hormone 1 Rattus norvegicus 127-131 10499517-7 1999 Using fetal explants, the NMDA-evoked release of GnRH was potentiated in a dose-dependent manner by bacitracin, a competitive PEP inhibitor and the desensitization to the NMDA effect was prevented using 2 mM of bacitracin. N-Methylaspartate 26-30 gonadotropin releasing hormone 1 Rattus norvegicus 49-53 10499517-7 1999 Using fetal explants, the NMDA-evoked release of GnRH was potentiated in a dose-dependent manner by bacitracin, a competitive PEP inhibitor and the desensitization to the NMDA effect was prevented using 2 mM of bacitracin. N-Methylaspartate 171-175 gonadotropin releasing hormone 1 Rattus norvegicus 49-53 9089469-2 1997 Using antisense oligodeoxynucleotides we aimed to study the role of N-methyl-D-aspartate (NMDA) and gamma-aminobutyric acid (GABA) receptors in the mechanism of Gonadotrophin-releasing hormone (GnRH) secretion in vitro. N-Methylaspartate 68-88 gonadotropin releasing hormone 1 Rattus norvegicus 161-192 9089469-2 1997 Using antisense oligodeoxynucleotides we aimed to study the role of N-methyl-D-aspartate (NMDA) and gamma-aminobutyric acid (GABA) receptors in the mechanism of Gonadotrophin-releasing hormone (GnRH) secretion in vitro. N-Methylaspartate 68-88 gonadotropin releasing hormone 1 Rattus norvegicus 194-198 9089469-2 1997 Using antisense oligodeoxynucleotides we aimed to study the role of N-methyl-D-aspartate (NMDA) and gamma-aminobutyric acid (GABA) receptors in the mechanism of Gonadotrophin-releasing hormone (GnRH) secretion in vitro. N-Methylaspartate 90-94 gonadotropin releasing hormone 1 Rattus norvegicus 161-192 9089469-4 1997 When GnRH secretion is evoked by muscimol and NMDA, a time-related reduction of GnRH secretion is caused by antisense oligodeoxynucleotides for the beta subunit of the GABAA receptor and the NR2A subunit of the NMDA receptor, respectively. N-Methylaspartate 46-50 gonadotropin releasing hormone 1 Rattus norvegicus 5-9 9089469-4 1997 When GnRH secretion is evoked by muscimol and NMDA, a time-related reduction of GnRH secretion is caused by antisense oligodeoxynucleotides for the beta subunit of the GABAA receptor and the NR2A subunit of the NMDA receptor, respectively. N-Methylaspartate 46-50 gonadotropin releasing hormone 1 Rattus norvegicus 80-84 9089469-7 1997 Using several missense oligodeoxynucleotides or antisense for the NR2B and NR2C subunits of the NMDA receptor, the muscimol- and NMDA-evoked release of GnRH is not affected. N-Methylaspartate 96-100 gonadotropin releasing hormone 1 Rattus norvegicus 152-156 9089470-8 1997 At the three studied ages, the NMDA-evoked release of GnRH and the GnRh interpulse interval are similarly inhibited by 100 or 500 microM of the NMDA receptor antagonist 7-chlorokynurenic acid (7CK). N-Methylaspartate 31-35 gonadotropin releasing hormone 1 Rattus norvegicus 54-58 9089470-8 1997 At the three studied ages, the NMDA-evoked release of GnRH and the GnRh interpulse interval are similarly inhibited by 100 or 500 microM of the NMDA receptor antagonist 7-chlorokynurenic acid (7CK). N-Methylaspartate 31-35 gonadotropin releasing hormone 1 Rattus norvegicus 67-71 8757241-6 1996 The acceleration of the elevation in GnRH mRNA levels by NMDA suggests that a stimulation of GnRH gene expression may be a rate-limiting factor for the onset of puberty. N-Methylaspartate 57-61 gonadotropin releasing hormone 1 Rattus norvegicus 93-97 7667416-8 1995 LH release evoked by either NMDA and LHRH was blocked by the LHRH antagonist. N-Methylaspartate 28-32 gonadotropin releasing hormone 1 Rattus norvegicus 61-65