PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 20404150-4 2010 To investigate the role of Golgi PtdIns4P in orchestrating trafficking events, we used a unique drug-inducible molecular approach to rapidly deplete PtdIns4P from Golgi membranes by a recruitable Sac1 phosphatase enzyme. phosphatidylinositol 4-phosphate 33-41 SAC1 like phosphatidylinositide phosphatase Homo sapiens 196-200 20404150-4 2010 To investigate the role of Golgi PtdIns4P in orchestrating trafficking events, we used a unique drug-inducible molecular approach to rapidly deplete PtdIns4P from Golgi membranes by a recruitable Sac1 phosphatase enzyme. phosphatidylinositol 4-phosphate 149-157 SAC1 like phosphatidylinositide phosphatase Homo sapiens 196-200 17908202-2 2007 The Sac1 lipid phosphatase regulates endoplasmic reticulum (ER) and Golgi phosphatidylinositol-4-phosphate [PI(4)P] in response to nutrient levels and cell growth stages. phosphatidylinositol 4-phosphate 74-106 SAC1 like phosphatidylinositide phosphatase Homo sapiens 4-8 15634212-0 2005 Regulation of intracellular phosphatidylinositol-4-phosphate by the Sac1 lipid phosphatase. phosphatidylinositol 4-phosphate 28-60 SAC1 like phosphatidylinositide phosphatase Homo sapiens 68-72 15634212-3 2005 Here we show that the polyphosphoinositide phosphatase Sac1p has a central role in compartment-specific regulation of PtdIns(4)P. phosphatidylinositol 4-phosphate 118-128 SAC1 like phosphatidylinositide phosphatase Homo sapiens 55-60 15634212-7 2005 In addition, sac1 mutants displayed elevated endoplasmic reticulum PtdIns(4)P. phosphatidylinositol 4-phosphate 67-77 SAC1 like phosphatidylinositide phosphatase Homo sapiens 13-17 15634212-9 2005 Our results indicate that elimination of Sac1p causes accumulation of a Stt4p-specific PtdIns(4)P pool at internal membranes which impairs late endocytic and vacuolar trafficking. phosphatidylinositol 4-phosphate 87-97 SAC1 like phosphatidylinositide phosphatase Homo sapiens 41-46 15634212-10 2005 We conclude that Sac1p functions in confining PtdIns(4)P-dependent processes to specific intracellular membranes. phosphatidylinositol 4-phosphate 46-56 SAC1 like phosphatidylinositide phosphatase Homo sapiens 17-22 30476538-2 2019 We have shown that exogenous H2O2 reversibly inactivates the phosphatidylinositol 4-phosphate [PtdIns(4)P] phosphatase Sac1 (suppressor of actin 1) at the Golgi complex of mammalian cells by oxidizing its catalytic cysteine residue and thereby increases both the amount of Golgi PtdIns(4)P and the rate of protein secretion. phosphatidylinositol 4-phosphate 61-93 SAC1 like phosphatidylinositide phosphatase Homo sapiens 119-123 10224048-4 1999 Purified recombinant SAC1-like domains convert yeast lipids phosphatidylinositol (PI) 3-phosphate, PI 4-phosphate, and PI 3,5-bisphosphate to PI, whereas PI 4,5-bisphosphate is not a substrate. phosphatidylinositol 4-phosphate 99-113 SAC1 like phosphatidylinositide phosphatase Homo sapiens 21-25 34320354-0 2021 SAC1 regulates autophagosomal phosphatidylinositol-4-phosphate for xenophagy-directed bacterial clearance. phosphatidylinositol 4-phosphate 30-62 SAC1 like phosphatidylinositide phosphatase Homo sapiens 0-4 34320354-5 2021 Mechanistically, the loss of SAC1 results in aberrant accumulation of phosphatidylinositol-4-phosphate (PI(4)P) on Salmonella-containing autophagosomes, thus facilitating recruitment of SteA, a PI(4)P-binding Salmonella effector protein, which impedes lysosomal fusion. phosphatidylinositol 4-phosphate 70-102 SAC1 like phosphatidylinositide phosphatase Homo sapiens 29-33 34812102-6 2022 Time-dependent, quantitative and live confocal imaging demonstrated that SACM1L-deficient cells accumulate phosphatidylinositol-4-phosphate (PtdIns4P) on bacteria-containing autophagosomes, resulting in delayed fusion with degradative lysosomes and reduced bacterial killing. phosphatidylinositol 4-phosphate 107-139 SAC1 like phosphatidylinositide phosphatase Homo sapiens 73-79 34812102-6 2022 Time-dependent, quantitative and live confocal imaging demonstrated that SACM1L-deficient cells accumulate phosphatidylinositol-4-phosphate (PtdIns4P) on bacteria-containing autophagosomes, resulting in delayed fusion with degradative lysosomes and reduced bacterial killing. phosphatidylinositol 4-phosphate 141-149 SAC1 like phosphatidylinositide phosphatase Homo sapiens 73-79 32269127-2 2020 In this study, we found that deletion or depletion of Sac1 phosphatidylinositol 4-phosphate [PI(4)P] phosphatase reduced tomato bushy stunt virus (TBSV) replication in yeast (Saccharomyces cerevisiae) and plants. phosphatidylinositol 4-phosphate 59-91 SAC1 like phosphatidylinositide phosphatase Homo sapiens 54-58 30476538-2 2019 We have shown that exogenous H2O2 reversibly inactivates the phosphatidylinositol 4-phosphate [PtdIns(4)P] phosphatase Sac1 (suppressor of actin 1) at the Golgi complex of mammalian cells by oxidizing its catalytic cysteine residue and thereby increases both the amount of Golgi PtdIns(4)P and the rate of protein secretion. phosphatidylinositol 4-phosphate 61-93 SAC1 like phosphatidylinositide phosphatase Homo sapiens 125-146 30476538-2 2019 We have shown that exogenous H2O2 reversibly inactivates the phosphatidylinositol 4-phosphate [PtdIns(4)P] phosphatase Sac1 (suppressor of actin 1) at the Golgi complex of mammalian cells by oxidizing its catalytic cysteine residue and thereby increases both the amount of Golgi PtdIns(4)P and the rate of protein secretion. phosphatidylinositol 4-phosphate 95-105 SAC1 like phosphatidylinositide phosphatase Homo sapiens 119-123 30476538-2 2019 We have shown that exogenous H2O2 reversibly inactivates the phosphatidylinositol 4-phosphate [PtdIns(4)P] phosphatase Sac1 (suppressor of actin 1) at the Golgi complex of mammalian cells by oxidizing its catalytic cysteine residue and thereby increases both the amount of Golgi PtdIns(4)P and the rate of protein secretion. phosphatidylinositol 4-phosphate 95-105 SAC1 like phosphatidylinositide phosphatase Homo sapiens 125-146 30476538-2 2019 We have shown that exogenous H2O2 reversibly inactivates the phosphatidylinositol 4-phosphate [PtdIns(4)P] phosphatase Sac1 (suppressor of actin 1) at the Golgi complex of mammalian cells by oxidizing its catalytic cysteine residue and thereby increases both the amount of Golgi PtdIns(4)P and the rate of protein secretion. phosphatidylinositol 4-phosphate 279-289 SAC1 like phosphatidylinositide phosphatase Homo sapiens 119-123 30476538-2 2019 We have shown that exogenous H2O2 reversibly inactivates the phosphatidylinositol 4-phosphate [PtdIns(4)P] phosphatase Sac1 (suppressor of actin 1) at the Golgi complex of mammalian cells by oxidizing its catalytic cysteine residue and thereby increases both the amount of Golgi PtdIns(4)P and the rate of protein secretion. phosphatidylinositol 4-phosphate 279-289 SAC1 like phosphatidylinositide phosphatase Homo sapiens 125-146 29461204-0 2018 SAC1 degrades its lipid substrate PtdIns4P in the endoplasmic reticulum to maintain a steep chemical gradient with donor membranes. phosphatidylinositol 4-phosphate 34-42 SAC1 like phosphatidylinositide phosphatase Homo sapiens 0-4 30448513-0 2019 Accumulation of PtdIns(4)P at the Golgi mediated by reversible oxidation of the PtdIns(4)P phosphatase Sac1 by H2O2. phosphatidylinositol 4-phosphate 16-26 SAC1 like phosphatidylinositide phosphatase Homo sapiens 103-107 30448513-0 2019 Accumulation of PtdIns(4)P at the Golgi mediated by reversible oxidation of the PtdIns(4)P phosphatase Sac1 by H2O2. phosphatidylinositol 4-phosphate 80-90 SAC1 like phosphatidylinositide phosphatase Homo sapiens 103-107 30448513-7 2019 The oxidative inactivation of Sac1 results in the accumulation of PtdIns(4)P at the Golgi, with this effect also being supported by the H2O2-induced activation of p38 mitogen-activated protein kinase (MAPK), which was previously shown to promote the translocation of Sac1 from the Golgi to the endoplasmic reticulum. phosphatidylinositol 4-phosphate 66-76 SAC1 like phosphatidylinositide phosphatase Homo sapiens 30-34 29411923-1 2018 The lipid phosphatase Sac1 dephosphorylates phosphatidylinositol 4-phosphate (PI4P), thereby holding levels of this crucial membrane signaling molecule in check. phosphatidylinositol 4-phosphate 44-76 SAC1 like phosphatidylinositide phosphatase Homo sapiens 22-26 30111818-5 2019 This analysis identified the evolutionarily conserved phosphatidylinositol 4-kinase IIIalpha (PI4KIIIalpha), and SAC1 phosphatase that dephosphorylates phosphatidylinositol-4-phosphate as potential candidate CXCR4 regulators. phosphatidylinositol 4-phosphate 152-184 SAC1 like phosphatidylinositide phosphatase Homo sapiens 113-117 29461204-2 2018 This novel pathway requires the SAC1 phosphatase to degrade PtdIns4P in a "cis" configuration at the ER to maintain the gradient. phosphatidylinositol 4-phosphate 60-68 SAC1 like phosphatidylinositide phosphatase Homo sapiens 32-36 29461204-5 2018 We report that acute inhibition of SAC1 causes accumulation of PtdIns4P in the ER, that SAC1 does not enrich at membrane contact sites, and that SAC1 has little activity in "trans", unless a linker is added between its ER-anchored and catalytic domains. phosphatidylinositol 4-phosphate 63-71 SAC1 like phosphatidylinositide phosphatase Homo sapiens 35-39 30854527-5 2016 This increased PS in the ER in turn, activated the Sac1 phosphatase, which is responsible for the dephosphorylation of the minor lipid, phosphatidylinositol 4-phosphate (PI4P) in the ER. phosphatidylinositol 4-phosphate 136-168 SAC1 like phosphatidylinositide phosphatase Homo sapiens 51-55 27178239-4 2016 In this study, we showed that an increase in PI(4)P levels at the Golgi by knockdown of PI(4)P phosphatase SAC1 increased the expression of standard CD44, variant CD44, and ezrin/radixin phosphorylation and enhanced the formation of focal adhesions mediated by CD44 and ezrin/radixin in MCF7 and SK-BR-3 cells. phosphatidylinositol 4-phosphate 45-51 SAC1 like phosphatidylinositide phosphatase Homo sapiens 107-111 27044099-4 2016 Here we show that expression of mutant PSS1 enzymes decreased phosphatidylinositol 4-phosphate (PI4P) levels both in the Golgi and the plasma membrane (PM) by activating the Sac1 phosphatase and altered PI4P cycling at the PM. phosphatidylinositol 4-phosphate 62-94 SAC1 like phosphatidylinositide phosphatase Homo sapiens 174-178 27044890-4 2016 Here, Sac1 participates in phosphoinositide homeostasis by limiting PM phosphatidylinositol 4-phosphate (PI(4)P), the precursor of PI(4,5)P2 Activation of G protein-coupled receptors that deplete PM PI(4,5)P2disrupts E-Syt2-mediated ER-PM junctions, reducing Sac1"s access to the PM and permitting PM PI(4)P and PI(4,5)P2to recover. phosphatidylinositol 4-phosphate 71-103 SAC1 like phosphatidylinositide phosphatase Homo sapiens 6-10 27044890-4 2016 Here, Sac1 participates in phosphoinositide homeostasis by limiting PM phosphatidylinositol 4-phosphate (PI(4)P), the precursor of PI(4,5)P2 Activation of G protein-coupled receptors that deplete PM PI(4,5)P2disrupts E-Syt2-mediated ER-PM junctions, reducing Sac1"s access to the PM and permitting PM PI(4)P and PI(4,5)P2to recover. phosphatidylinositol 4-phosphate 105-111 SAC1 like phosphatidylinositide phosphatase Homo sapiens 6-10 27044890-4 2016 Here, Sac1 participates in phosphoinositide homeostasis by limiting PM phosphatidylinositol 4-phosphate (PI(4)P), the precursor of PI(4,5)P2 Activation of G protein-coupled receptors that deplete PM PI(4,5)P2disrupts E-Syt2-mediated ER-PM junctions, reducing Sac1"s access to the PM and permitting PM PI(4)P and PI(4,5)P2to recover. phosphatidylinositol 4-phosphate 105-111 SAC1 like phosphatidylinositide phosphatase Homo sapiens 259-263 27044890-4 2016 Here, Sac1 participates in phosphoinositide homeostasis by limiting PM phosphatidylinositol 4-phosphate (PI(4)P), the precursor of PI(4,5)P2 Activation of G protein-coupled receptors that deplete PM PI(4,5)P2disrupts E-Syt2-mediated ER-PM junctions, reducing Sac1"s access to the PM and permitting PM PI(4)P and PI(4,5)P2to recover. phosphatidylinositol 4-phosphate 301-307 SAC1 like phosphatidylinositide phosphatase Homo sapiens 6-10 27044890-5 2016 Conversely, depletion of ER luminal calcium and subsequent activation of SOCE increases the amount of Sac1 in contact with the PM, depleting PM PI(4)P. phosphatidylinositol 4-phosphate 144-150 SAC1 like phosphatidylinositide phosphatase Homo sapiens 102-106 22553352-0 2012 Local control of phosphatidylinositol 4-phosphate signaling in the Golgi apparatus by Vps74 and Sac1 phosphoinositide phosphatase. phosphatidylinositol 4-phosphate 17-49 SAC1 like phosphatidylinositide phosphatase Homo sapiens 96-100 25869669-2 2015 Here, we demonstrate that the Sac1 domain-containing protein Sac2 is a PI 4-phosphatase that specifically hydrolyzes phosphatidylinositol 4-phosphate in vitro. phosphatidylinositol 4-phosphate 117-149 SAC1 like phosphatidylinositide phosphatase Homo sapiens 30-34 25113029-1 2014 Sac1 is a phosphoinositide phosphatase of the endoplasmic reticulum and Golgi apparatus that controls organelle membrane composition principally via regulation of phosphatidylinositol 4-phosphate signaling. phosphatidylinositol 4-phosphate 163-195 SAC1 like phosphatidylinositide phosphatase Homo sapiens 0-4 25113029-4 2014 Disruption of the Sac1-Vps74 interface results in a broader distribution of phosphatidylinositol 4-phosphate within the Golgi apparatus and failure to maintain residence of a medial Golgi mannosyltransferase. phosphatidylinositol 4-phosphate 76-108 SAC1 like phosphatidylinositide phosphatase Homo sapiens 18-22 22553352-3 2012 Vps74 binds directly to the catalytic domain of Sac1 (K(D) = 3.8 muM), the major PtdIns4P phosphatase in the cell, and PtdIns4P is elevated on medial Golgi cisternae in cells lacking Vps74 or Sac1, suggesting that Vps74 is a sensor of PtdIns4P level on medial Golgi cisternae that directs Sac1-mediated dephosphosphorylation of this pool of PtdIns4P. phosphatidylinositol 4-phosphate 81-89 SAC1 like phosphatidylinositide phosphatase Homo sapiens 48-52 22553352-3 2012 Vps74 binds directly to the catalytic domain of Sac1 (K(D) = 3.8 muM), the major PtdIns4P phosphatase in the cell, and PtdIns4P is elevated on medial Golgi cisternae in cells lacking Vps74 or Sac1, suggesting that Vps74 is a sensor of PtdIns4P level on medial Golgi cisternae that directs Sac1-mediated dephosphosphorylation of this pool of PtdIns4P. phosphatidylinositol 4-phosphate 119-127 SAC1 like phosphatidylinositide phosphatase Homo sapiens 48-52 22553352-3 2012 Vps74 binds directly to the catalytic domain of Sac1 (K(D) = 3.8 muM), the major PtdIns4P phosphatase in the cell, and PtdIns4P is elevated on medial Golgi cisternae in cells lacking Vps74 or Sac1, suggesting that Vps74 is a sensor of PtdIns4P level on medial Golgi cisternae that directs Sac1-mediated dephosphosphorylation of this pool of PtdIns4P. phosphatidylinositol 4-phosphate 119-127 SAC1 like phosphatidylinositide phosphatase Homo sapiens 48-52 22260691-4 2012 Growth-dependent translocation of Sac1 between the ER and Golgi modulates the levels of PtdIns4P and anterograde traffic at the Golgi. phosphatidylinositol 4-phosphate 88-96 SAC1 like phosphatidylinositide phosphatase Homo sapiens 34-38 22260691-6 2012 Sac1 lipid phosphatase plays also an essential role in the spatial control of PtdIns4P at the Golgi complex. phosphatidylinositol 4-phosphate 78-86 SAC1 like phosphatidylinositide phosphatase Homo sapiens 0-4 21119004-4 2011 Analysis of wild type and mutant PH domain proteins in addition to recombinant versions of the Sac1p phosphoinositide-phosphatase indicated that PI(4)P was required on Golgi membranes for fusion with coat protein complex II (COPII) vesicles. phosphatidylinositol 4-phosphate 145-151 SAC1 like phosphatidylinositide phosphatase Homo sapiens 95-100 20573065-2 2010 Here, we show that lipid phosphatase Suppressor of actin mutations 1 (SAC1) is crucial for the spatial regulation of Golgi PI(4)P. phosphatidylinositol 4-phosphate 123-129 SAC1 like phosphatidylinositide phosphatase Homo sapiens 37-68 20573065-2 2010 Here, we show that lipid phosphatase Suppressor of actin mutations 1 (SAC1) is crucial for the spatial regulation of Golgi PI(4)P. phosphatidylinositol 4-phosphate 123-129 SAC1 like phosphatidylinositide phosphatase Homo sapiens 70-74 20573065-3 2010 Ultrastructural analysis revealed that SAC1 is predominantly located at cisternal Golgi membranes but is absent from the TGN, thus confining PI(4)P to the TGN. phosphatidylinositol 4-phosphate 141-147 SAC1 like phosphatidylinositide phosphatase Homo sapiens 39-43 20573065-7 2010 These results indicate that SAC1 organizes PI(4)P distribution between the Golgi complex and the TGN, which is instrumental for resident enzyme partitioning and Golgi morphology. phosphatidylinositol 4-phosphate 43-49 SAC1 like phosphatidylinositide phosphatase Homo sapiens 28-32