PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 2187531-7 1990 GN-binding activity of Gt-I preparations was dependent on reaction temperature, and no binding was observed at 4 degrees C. In the presence of 10 microM bleached rhodopsin, Gt-I preparations bound GTP gamma S at 4 degrees C. However, hexylagarose chromatography of Gt-I preparations led to a preparation of Gt that showed less than 0.1 mol/mol binding activity following 60-min incubation at 30 degrees C in the absence of rhodopsin (Gt-II preparations). aminohexyl-sepharose 234-246 rhodopsin Bos taurus 162-171