PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 9774389-6 1998 Part of the [14C]arachidonic acid-labeled PE transferred from low density lipoprotein to platelets activated by thrombin and collagen was metabolized to 14C-eicosanoids. Carbon-14 13-16 coagulation factor II, thrombin Homo sapiens 112-120 10527409-8 1999 Aggregation and release of residual [14C]serotonin in response to 0.1-1.0 U/mL thrombin was blocked or greatly reduced by the cathepsin G pretreatment. Carbon-14 37-40 coagulation factor II, thrombin Homo sapiens 79-87 9814996-6 1998 After stimulation with thrombin (0.5 U/ml), 7.1% ([14C]AA-PC) and 10.6% ([14C]AA-phosphatidylethanolamine) of the 14C transferred from LDL to the platelets were recovered in the eicosanoids [14C]thromboxane B2 (TxB2) plus 12-[14C]hydroxyeicosatetraenoic acid. Carbon-14 51-54 coagulation factor II, thrombin Homo sapiens 23-31 9814996-6 1998 After stimulation with thrombin (0.5 U/ml), 7.1% ([14C]AA-PC) and 10.6% ([14C]AA-phosphatidylethanolamine) of the 14C transferred from LDL to the platelets were recovered in the eicosanoids [14C]thromboxane B2 (TxB2) plus 12-[14C]hydroxyeicosatetraenoic acid. Carbon-14 74-77 coagulation factor II, thrombin Homo sapiens 23-31 9814996-6 1998 After stimulation with thrombin (0.5 U/ml), 7.1% ([14C]AA-PC) and 10.6% ([14C]AA-phosphatidylethanolamine) of the 14C transferred from LDL to the platelets were recovered in the eicosanoids [14C]thromboxane B2 (TxB2) plus 12-[14C]hydroxyeicosatetraenoic acid. Carbon-14 74-77 coagulation factor II, thrombin Homo sapiens 23-31 9774389-7 1998 Inhibitors of protein kinase C partially prevented thrombin-induced [14C]PE uptake, while direct activators of protein kinase C increased incorporation of [14C]PE into platelets. Carbon-14 69-72 coagulation factor II, thrombin Homo sapiens 51-59 7556592-6 1995 Both thrombin and SFLLRN caused complete release of beta-thromboglobulin and 88.3 and 77.5% release of [14C]serotonin, respectively. Carbon-14 104-107 coagulation factor II, thrombin Homo sapiens 5-13 9134653-4 1997 Incubation with 0.2 CU/ml of plasmin almost completely inhibited thrombin-induced (0.1 U/ml) aggregation, release of 14C-serotonin, and increase in cytosolic [Ca2+]. Carbon-14 117-120 coagulation factor II, thrombin Homo sapiens 65-73 9070908-3 1997 [14C]N,N-dimethylsphingosine was stable in resting platelets, while it was converted into N,N-dimethylsphingosine 1-phosphate (DMS-1-P), although weakly, in platelets stimulated with thrombin or 12-O-tetradecanoylphorbol 13-acetate. Carbon-14 1-4 coagulation factor II, thrombin Homo sapiens 183-191 2548563-9 1989 As compared with TPP and the other subpopulations, LD platelets were most sensitive upon ADP and thrombin stimulated [14C]serotonin release as well as upon ADP, serotonin and thrombin induced aggregation. Carbon-14 118-121 coagulation factor II, thrombin Homo sapiens 97-105 8199173-2 1994 The native chyle chylomicrons did not induce platelet aggregation but decreased ADP- and thrombin-induced platelet aggregation and [14C]serotonin release. Carbon-14 132-135 coagulation factor II, thrombin Homo sapiens 89-97 8199173-3 1994 Chylomicron remnants also failed to induce platelet aggregation, but they potentiated the aggregation and the [14C]serotonin release induced by ADP and thrombin. Carbon-14 111-114 coagulation factor II, thrombin Homo sapiens 152-160 7740483-3 1995 We compared responses of 14C-serotonin-labeled, chymotrypsin-treated platelets (and control platelets) to thrombin (0.25-2 U/ml) and SFLLRN (5-40 microM). Carbon-14 25-28 coagulation factor II, thrombin Homo sapiens 106-114 7740483-8 1995 Concentrations that abolished aggregation and release of 14C-serotonin in response to thrombin had little effect on these responses to SFLLRN. Carbon-14 57-60 coagulation factor II, thrombin Homo sapiens 86-94 8011429-3 1994 These drugs also inhibited thrombin-induced [14C]serotonin secretion of platelets. Carbon-14 45-48 coagulation factor II, thrombin Homo sapiens 27-35 7526105-2 1994 In this study, we confirmed the inhibitory effect of HDL on platelet aggregation and 14C-serotonin release in thrombin-activated washed human platelets. Carbon-14 85-88 coagulation factor II, thrombin Homo sapiens 110-118 2172436-4 1990 Co-incubation of [1-14C]arachidonate-labeled platelets with primed polymorphonuclear leukocytes (PMN) followed by addition of thrombin and FMLP led to the formation of both 5- and 15-LO products that carried 14C label. Carbon-14 20-23 coagulation factor II, thrombin Homo sapiens 126-134 1697614-5 1990 Further studies of its effect on thrombin-induced activities showed that serum amyloid A, at concentrations of 25 to 100 micrograms/ml (which are found in mild acute events), suppressed the increase of cytosolic [Ca2+], thromboxane generation, and carbon 14-labeled serotonin release in a dose-dependent fashion. Carbon-14 248-257 coagulation factor II, thrombin Homo sapiens 33-41 2227132-6 1990 In response to a low concentration of thrombin, mean values for release of [14C]serotonin from washed prelabeled platelets were not significantly different between diabetic and control subjects, but in 8 of 12 diabetic subjects, the release response was greater than in their paired control subjects. Carbon-14 76-79 coagulation factor II, thrombin Homo sapiens 38-46 2794047-9 1989 This stands in contrast to 125I-FGF (fibroblast growth factor) binding to ECM, which was inhibited by heparitinase but not by chondroitinase ABC, ECM-bound thrombin exhibits an exposed proteolytic site as monitored by the Chromozyme TH assay and by its ability to convert fibrinogen to a fibrin clot and to induce platelet activation as indicated by 14C-serotonin release. Carbon-14 350-353 coagulation factor II, thrombin Homo sapiens 156-164 2521636-6 1989 To test this hypothesis, human endothelial cells labeled with [14C]arachidonic acid were stimulated with thrombin (2 units/ml, 10 min), resulting in the secretion of free arachidonic acid together with various 14C-labeled metabolites, mainly 6-keto-prostaglandin F1 alpha, the stable derivative of prostaglandin I2. Carbon-14 63-66 coagulation factor II, thrombin Homo sapiens 105-113 2521636-6 1989 To test this hypothesis, human endothelial cells labeled with [14C]arachidonic acid were stimulated with thrombin (2 units/ml, 10 min), resulting in the secretion of free arachidonic acid together with various 14C-labeled metabolites, mainly 6-keto-prostaglandin F1 alpha, the stable derivative of prostaglandin I2. Carbon-14 210-213 coagulation factor II, thrombin Homo sapiens 105-113 3038150-1 1987 The thrombin-dependent enrichment of alkenylacyl ethanolamine phosphoglyceride in [14C]eicosapentaenoic acid [( 14C]EPA) was demonstrated and compared with [3H]arachidonic acid [( 3H]AA) following the simultaneous prelabelling of individual human platelet phospholipids with these two fatty acids. Carbon-14 83-87 coagulation factor II, thrombin Homo sapiens 4-12 3110996-6 1987 However, the inhibition of maximal thrombin-induced [14C]-5HT secretion and TxB2 synthesis, which are both aggregation-independent phenomena, could be attributed to the inhibition of thrombin-induced diacylglycerol formation and intracellular calcium mobilization, which were both inhibited by 80% in the presence of spermine. Carbon-14 53-56 coagulation factor II, thrombin Homo sapiens 35-43 3110996-6 1987 However, the inhibition of maximal thrombin-induced [14C]-5HT secretion and TxB2 synthesis, which are both aggregation-independent phenomena, could be attributed to the inhibition of thrombin-induced diacylglycerol formation and intracellular calcium mobilization, which were both inhibited by 80% in the presence of spermine. Carbon-14 53-56 coagulation factor II, thrombin Homo sapiens 183-191 2840908-5 1988 Modifications of [14C]arachidonic acid metabolism in human platelets stimulated by thrombin were then measured in the presence of drugs. Carbon-14 18-21 coagulation factor II, thrombin Homo sapiens 83-91 3038150-1 1987 The thrombin-dependent enrichment of alkenylacyl ethanolamine phosphoglyceride in [14C]eicosapentaenoic acid [( 14C]EPA) was demonstrated and compared with [3H]arachidonic acid [( 3H]AA) following the simultaneous prelabelling of individual human platelet phospholipids with these two fatty acids. Carbon-14 83-86 coagulation factor II, thrombin Homo sapiens 4-12 3771562-10 1986 The substitution of cysteine for arginine was confirmed by tryptic digestion of 14C-carboxymethylated prothrombin Barcelona. Carbon-14 80-83 coagulation factor II, thrombin Homo sapiens 102-113 3801503-4 1987 Analysis of released 14C-fatty acids by radio-gas chromatography indicated that thrombin stimulated the release of 6-10% of the [14C]arachidonate synthesized by desaturation of the [14C]eicosatrienoate, but did not stimulate release of [14C]eicosatrienoate per se (less than 1%). Carbon-14 21-24 coagulation factor II, thrombin Homo sapiens 80-88 3032217-6 1986 The [3H]/[14C] ratios for the increased radioactivity appearing in diacylglycerol (DG) and phosphatidic acid (PA) upon thrombin stimulation were not significantly different from the corresponding ratio in phosphatidylinositol (PI) from resting platelets, suggesting little or no preference for 1-acyl-2-eicosapentaenoyl PI over 1-acyl-2-arachidonoyl PI in the pathway from PI to DG to PA. Carbon-14 10-13 coagulation factor II, thrombin Homo sapiens 119-127 2877688-4 1986 Proteolytic digestion of [14C]putrescine-labeled TSP with trypsin or thrombin yielded a labeled disulfide-bonded core of 90 or 120-130 kilodalton (kDa) subunits, labeled fragments of less than 10 kDa, and an unlabeled 30-kDa heparin-binding fragment, indicating the presence of multiple factor XIIIa reactive glutaminyl residues located in several domains of the molecule. Carbon-14 26-29 coagulation factor II, thrombin Homo sapiens 69-77 3012818-7 1986 Aggregation and 14C-serotonin secretion stimulated by 0.1 U/ml thrombin were partially inhibited by pretreatment with bimane. Carbon-14 16-19 coagulation factor II, thrombin Homo sapiens 63-71 3949008-3 1986 However, addition of OAG (10-50 micrograms/ml) 10 s to 5 min before or 10-60 s after addition of threshold concentrations of thrombin (less than 0.03 U/ml) resulted in a significant potentiation of aggregation and [14C]5HT secretion without any effect on the thrombin-induced rise in [Ca2+]i. Carbon-14 215-218 coagulation factor II, thrombin Homo sapiens 125-133 3949008-4 1986 Both EGTA, which abolished the latter and creatine phosphate/creatine phosphokinase, the ADP scavenger, totally inhibited the aggregation but only partially reduced [14C]5HT secretion in response to thrombin plus OAG. Carbon-14 166-169 coagulation factor II, thrombin Homo sapiens 199-207 3949008-6 1986 The results demonstrate that, unlike phorbol esters, OAG has no inhibitory effect on thrombin-induced [Ca2+]i mobilisation but can synergize with low concentrations of thrombin in potentiating [14C]5HT secretion even at basal [Ca2+]i. Carbon-14 194-197 coagulation factor II, thrombin Homo sapiens 168-176 6437832-6 1984 Washed platelets prelabelled with [14C]-AA increased radioactivity 10-fold in free fatty acids (FFA) by exposure to thrombin [2 U (10(9) platelets)-1] for 20 s. This increase was augmented by cod liver oil. Carbon-14 35-38 coagulation factor II, thrombin Homo sapiens 116-124 3006686-4 1986 PDGF inhibited thrombin and collagen induced platelet aggregation and 14C-serotonin release in a dose dependent manner, but was without effect when arachidonic acid was used. Carbon-14 70-73 coagulation factor II, thrombin Homo sapiens 15-23 3085207-4 1986 Thrombin induced a slightly decreased aggregation and a normal 14C-serotonin release. Carbon-14 63-66 coagulation factor II, thrombin Homo sapiens 0-8 3752924-8 1986 Plasma chylomicrons prepared from both groups during alimentary hyperlipemia inhibited ADP-induced platelet aggregation as well as thrombin-induced platelet 14C-serotonin release. Carbon-14 157-160 coagulation factor II, thrombin Homo sapiens 131-139 2409615-4 1985 Gi3 and Gi5 blocked platelet aggregation and 14C-serotonin release from platelets induced by ADP, collagen, thrombin, arachidonic acid, ionophore A 23187, platelet activating factor and ristocetin (in the presence of divalent ions). Carbon-14 45-48 coagulation factor II, thrombin Homo sapiens 108-116 3925115-3 1985 Dilazep decreased thrombin-induced release of [14C]arachidonic acid ([14C]AA) from platelets prelabeled with [14C]AA. Carbon-14 47-50 coagulation factor II, thrombin Homo sapiens 18-26 3925115-3 1985 Dilazep decreased thrombin-induced release of [14C]arachidonic acid ([14C]AA) from platelets prelabeled with [14C]AA. Carbon-14 70-73 coagulation factor II, thrombin Homo sapiens 18-26 3002485-4 1986 By contrast, after 24 h of fatty acid incorporation, the thrombin-stimulated release of radiolabeled fatty acid from cells incubated with [14C]eicosapentaenoate was only 25-30% of that from cells with [14C]arachidonate. Carbon-14 139-142 coagulation factor II, thrombin Homo sapiens 57-65 3002485-4 1986 By contrast, after 24 h of fatty acid incorporation, the thrombin-stimulated release of radiolabeled fatty acid from cells incubated with [14C]eicosapentaenoate was only 25-30% of that from cells with [14C]arachidonate. Carbon-14 202-205 coagulation factor II, thrombin Homo sapiens 57-65 3923646-3 1985 Thrombin stimulated the loss of 3H and 14C label from intracellular phospholipids. Carbon-14 39-42 coagulation factor II, thrombin Homo sapiens 0-8 6236084-3 1984 In contrast, thrombin shifts the dose/response curve for Ca2+-induced [14C]serotonin secretion to the left and has little effect on the maximal extent of this response even when this extent is reduced by use of a non-saturating concentration of MgATP2-. Carbon-14 71-74 coagulation factor II, thrombin Homo sapiens 13-21 6236084-6 1984 Activators of protein kinase C have previously been shown to mimic quantitatively the effect of thrombin on [14C]serotonin secretion induced by Ca2+ [D. E. Knight & M. C. Scrutton (1984) Nature (Lond.) Carbon-14 109-112 coagulation factor II, thrombin Homo sapiens 96-104 6236084-9 1984 The EC50 for thrombin observed for enhancement of [14C]serotonin and beta-N-acetylglucosaminidase secretion is in the same range as that obtained for intact platelets under comparable conditions [D. E. Knight, T. J. Hallam & M. C. Scrutton (1982) Nature (Lond.) Carbon-14 51-54 coagulation factor II, thrombin Homo sapiens 13-21 6746652-1 1984 The relative degradation of the various molecular species of [3H]phosphatidylcholine in response to thrombin was studied in human platelets following prelabeling with [3H]glycerol and compared to results obtained following labeling with [14C]oleic, [14C]linoleic, or [14C]arachidonic acids. Carbon-14 238-241 coagulation factor II, thrombin Homo sapiens 100-108 6746652-1 1984 The relative degradation of the various molecular species of [3H]phosphatidylcholine in response to thrombin was studied in human platelets following prelabeling with [3H]glycerol and compared to results obtained following labeling with [14C]oleic, [14C]linoleic, or [14C]arachidonic acids. Carbon-14 250-253 coagulation factor II, thrombin Homo sapiens 100-108 6746652-1 1984 The relative degradation of the various molecular species of [3H]phosphatidylcholine in response to thrombin was studied in human platelets following prelabeling with [3H]glycerol and compared to results obtained following labeling with [14C]oleic, [14C]linoleic, or [14C]arachidonic acids. Carbon-14 250-253 coagulation factor II, thrombin Homo sapiens 100-108 6743305-2 1984 The assumption that the thrombin-platelet reaction shows characteristics of an agonist-receptor interaction was corroborated by an analysis of the relation between thrombin concentration and yield of 14C-serotonin secretion. Carbon-14 200-203 coagulation factor II, thrombin Homo sapiens 24-32 6428500-11 1984 Thrombin-induced aggregation was accompanied by substantial loss of 14C from phospholipids and equivalent gains of 14C in free fatty acids and TxB2; mepacrine pretreatment caused partial inhibition of thrombin-induced aggregation, halved the net 14C loss from phospholipids, but had little effect on the appearance of 14C in TxB2. Carbon-14 68-71 coagulation factor II, thrombin Homo sapiens 0-8 6428500-11 1984 Thrombin-induced aggregation was accompanied by substantial loss of 14C from phospholipids and equivalent gains of 14C in free fatty acids and TxB2; mepacrine pretreatment caused partial inhibition of thrombin-induced aggregation, halved the net 14C loss from phospholipids, but had little effect on the appearance of 14C in TxB2. Carbon-14 115-118 coagulation factor II, thrombin Homo sapiens 0-8 6428500-11 1984 Thrombin-induced aggregation was accompanied by substantial loss of 14C from phospholipids and equivalent gains of 14C in free fatty acids and TxB2; mepacrine pretreatment caused partial inhibition of thrombin-induced aggregation, halved the net 14C loss from phospholipids, but had little effect on the appearance of 14C in TxB2. Carbon-14 115-118 coagulation factor II, thrombin Homo sapiens 0-8 6428500-11 1984 Thrombin-induced aggregation was accompanied by substantial loss of 14C from phospholipids and equivalent gains of 14C in free fatty acids and TxB2; mepacrine pretreatment caused partial inhibition of thrombin-induced aggregation, halved the net 14C loss from phospholipids, but had little effect on the appearance of 14C in TxB2. Carbon-14 115-118 coagulation factor II, thrombin Homo sapiens 0-8 6743305-2 1984 The assumption that the thrombin-platelet reaction shows characteristics of an agonist-receptor interaction was corroborated by an analysis of the relation between thrombin concentration and yield of 14C-serotonin secretion. Carbon-14 200-203 coagulation factor II, thrombin Homo sapiens 164-172 6313646-2 1983 When bound to thrombomodulin, thrombin no longer induces platelets to either aggregate or release [14C] serotonin. Carbon-14 99-102 coagulation factor II, thrombin Homo sapiens 30-38 1159095-5 1975 These anion-induced changes in 125I-thrombin binding paralleled changes in [14C] serotonin release when both parameters were measured in the same platelets. Carbon-14 76-79 coagulation factor II, thrombin Homo sapiens 36-44 6311825-6 1983 In cholesterol-enriched, cholesterol-normal, and cholesterol-depleted platelets, the thrombin concentrations required for half-maximal aggregation were 0.17, 0.35, and 0.52 nM, respectively, while the values for half-maximal secretion of [14C]serotonin were 0.17, 0.40, and 0.55 nM, respectively. Carbon-14 239-242 coagulation factor II, thrombin Homo sapiens 85-93 6188232-2 1982 Addition of increasing concentrations of thrombin (0.015-0.625 U/ml) resulted in an immediate increase of 14C-NE detectable in the supernatants, but the slopes of the efflux curves from 10 min after thrombin addition were almost identical and parallel with the slope before thrombin addition. Carbon-14 106-109 coagulation factor II, thrombin Homo sapiens 41-49 7213342-2 1980 Cleavage of the human antithrombin III--thrombin complex with [14C]methoxyamine hydrochloride results in inactive thrombin and 14C-labelled antithrombin III. Carbon-14 63-66 coagulation factor II, thrombin Homo sapiens 26-34 7213342-2 1980 Cleavage of the human antithrombin III--thrombin complex with [14C]methoxyamine hydrochloride results in inactive thrombin and 14C-labelled antithrombin III. Carbon-14 63-66 coagulation factor II, thrombin Homo sapiens 40-48 7213342-2 1980 Cleavage of the human antithrombin III--thrombin complex with [14C]methoxyamine hydrochloride results in inactive thrombin and 14C-labelled antithrombin III. Carbon-14 127-130 coagulation factor II, thrombin Homo sapiens 26-34 7213342-2 1980 Cleavage of the human antithrombin III--thrombin complex with [14C]methoxyamine hydrochloride results in inactive thrombin and 14C-labelled antithrombin III. Carbon-14 127-130 coagulation factor II, thrombin Homo sapiens 40-48 458246-4 1979 Preincubation of dPRP with antimycin A and 2-deoxy-D-glucose before addition of thrombin prolonged the clot lysis to 26 hr and inhibited release of 14C-5-HT at the time of the temperature shift. Carbon-14 148-151 coagulation factor II, thrombin Homo sapiens 80-88 375988-5 1979 Increased turnover of [14C]- or [32P]phosphatidate occurs within 2--5 s after platelet activation by thrombin and is observed before endogenous, 14C-labeled arachidonate can be detected. Carbon-14 23-26 coagulation factor II, thrombin Homo sapiens 101-109 678668-7 1978 Unlike aggregation, ADP-induced shape change, clot retraction caused by thrombin or ADP plus reptilase, and thrombin-induced 14C-serotonin release were not inhibited after incubation. Carbon-14 125-128 coagulation factor II, thrombin Homo sapiens 108-116 204935-1 1978 Stimulation of platelets labeled with [14C]-arachidonate by ionophore A23187 or thrombin produces rapid degradation of specific membrane phospholipids. Carbon-14 39-42 coagulation factor II, thrombin Homo sapiens 80-88 618992-6 1978 The release of [14C]serotonin by thrombasthenic platelets as a function of thrombin concentration was also similar to release by normal platelets. Carbon-14 16-19 coagulation factor II, thrombin Homo sapiens 75-83 6615246-2 1983 The addition of red blood cells to platelet-rich plasma enhanced thrombin-induced [14C] serotonin release by 7%, whereas in a gel-filtered platelet preparation free of any plasma constituents a 47% increment was noted. Carbon-14 83-86 coagulation factor II, thrombin Homo sapiens 65-73 6615246-5 1983 Very low density lipoprotein and low density lipoprotein increased thrombin-induced platelet aggregation and [14C] serotonin release induced by epinephrine, ADP, and thrombin. Carbon-14 110-113 coagulation factor II, thrombin Homo sapiens 166-174 6811295-3 1982 Malondialdehyde formation, aggregation and [14C]serotonin release caused by threshold doses of thrombin were reduced but not abolished by 100 muM vinblastine; 30-100 muM vinblastine abolished ADP- and PGAP-induced malondialdehyde formation and [14C]serotonin released and transformed ADP- and PGAP-induced irreversible aggregation to a diminished reversible response. Carbon-14 44-47 coagulation factor II, thrombin Homo sapiens 95-103 6811295-3 1982 Malondialdehyde formation, aggregation and [14C]serotonin release caused by threshold doses of thrombin were reduced but not abolished by 100 muM vinblastine; 30-100 muM vinblastine abolished ADP- and PGAP-induced malondialdehyde formation and [14C]serotonin released and transformed ADP- and PGAP-induced irreversible aggregation to a diminished reversible response. Carbon-14 245-248 coagulation factor II, thrombin Homo sapiens 95-103 6289076-2 1982 The actions of ionophores with different ion specificities and of thrombin on the release of 14C-labeled 5-hydroxytryptamine, [3H]noradrenaline, and endogenous ATP were measured in human platelets suspended in media with various K+ and Na+ concentrations. Carbon-14 93-96 coagulation factor II, thrombin Homo sapiens 66-74 6795196-1 1981 Concentrations (1 to 20 microM) of 1-oleoyl-lysophosphatidic acid which alone do not affect platelet metabolism of arachidonic acid, do augment the effects of suboptimal concentrations of thrombin on the formation of [14C]phosphatidic acid and the production of [14C]arachidonate metabolites from platelets prelabeled with [14C]arachidonate. Carbon-14 217-222 coagulation factor II, thrombin Homo sapiens 188-196 6795196-1 1981 Concentrations (1 to 20 microM) of 1-oleoyl-lysophosphatidic acid which alone do not affect platelet metabolism of arachidonic acid, do augment the effects of suboptimal concentrations of thrombin on the formation of [14C]phosphatidic acid and the production of [14C]arachidonate metabolites from platelets prelabeled with [14C]arachidonate. Carbon-14 218-221 coagulation factor II, thrombin Homo sapiens 188-196 6795196-2 1981 The effect on [14C]phosphatidate occurs with concentrations of thrombin (0.1 unit/ml) which are lower than those (0.2 unit/ml) needed to observe the effects on [14C]arachidonate metabolites. Carbon-14 15-18 coagulation factor II, thrombin Homo sapiens 63-71