PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 8395564-5 1993 In an age-selected group (range, 10-89 years), the density of monoamine oxidase (MAO)-B sites labeled by [3H]Ro 19-6327 (lazabemide) also showed a positive correlation with age (r = 0.80; p < 0.005). Tritium 106-108 monoamine oxidase B Homo sapiens 62-87 9394121-2 1997 The density of MAO-B sites was quantified by the specific binding of the selective inhibitor [3H]Ro 19-6327 (lazabemide) (8 nM) to cortical membranes. Tritium 94-96 monoamine oxidase B Homo sapiens 15-20 9116589-5 1997 The density of brain MAO-B sites labeled by [3H]Ro 19-6327 (lazabemide) in suicides was no different to that in age-matched controls. Tritium 45-47 monoamine oxidase B Homo sapiens 21-26 8395564-8 1993 In the human frontal cortex, idazoxan displayed very low affinity (Ki = 89 microM) against the binding of [3H]Ro 19-6327 to MAO-B, which discounted a direct interaction of [3H]idazoxan with the active center of the enzyme and indicated that the I2-imidazoline site cannot be identified with MAO-B. Tritium 107-109 monoamine oxidase B Homo sapiens 124-129 1418862-6 1992 There was a high correlation between glial cell count and 3H-L-deprenyl binding with a relation indicating enhanced MAO-B protein in glial cells within areas of neurodegeneration. Tritium 58-60 monoamine oxidase B Homo sapiens 116-121 1321029-1 1992 Distribution of the enzyme monoamine oxidase B (MAO-B) and the peripheral benzodiazepine binding site (omega 3 site) was studied by quantitative autoradiography using [3H]L-deprenyl and [3H]PK 11195, two tentative glial markers, as ligands. Tritium 168-170 monoamine oxidase B Homo sapiens 27-46 1321029-1 1992 Distribution of the enzyme monoamine oxidase B (MAO-B) and the peripheral benzodiazepine binding site (omega 3 site) was studied by quantitative autoradiography using [3H]L-deprenyl and [3H]PK 11195, two tentative glial markers, as ligands. Tritium 187-189 monoamine oxidase B Homo sapiens 27-46 2314388-10 1990 As previously reported for the MAO-B ligands [3H]Ro 16-6491 and [3H]Ro 19-6327, the analysis of the membrane-bound radioactivity showed that [3H]Ro 41-1049 was entirely recovered in the form of its aldehyde derivative, indicating that Ro 41-1049 was deaminated by MAO-A. Tritium 46-48 monoamine oxidase B Homo sapiens 31-36 2314388-10 1990 As previously reported for the MAO-B ligands [3H]Ro 16-6491 and [3H]Ro 19-6327, the analysis of the membrane-bound radioactivity showed that [3H]Ro 41-1049 was entirely recovered in the form of its aldehyde derivative, indicating that Ro 41-1049 was deaminated by MAO-A. Tritium 65-67 monoamine oxidase B Homo sapiens 31-36 2314388-10 1990 As previously reported for the MAO-B ligands [3H]Ro 16-6491 and [3H]Ro 19-6327, the analysis of the membrane-bound radioactivity showed that [3H]Ro 41-1049 was entirely recovered in the form of its aldehyde derivative, indicating that Ro 41-1049 was deaminated by MAO-A. Tritium 65-67 monoamine oxidase B Homo sapiens 31-36 2297358-0 1990 Characterization of [3H]Ro 16-6491 binding to digitonin solubilized monoamine oxidase-B and purification of the enzyme from human platelets by affinity chromatography. Tritium 21-23 monoamine oxidase B Homo sapiens 68-87 3126263-0 1988 [3H]Ro 16-6491, a selective probe for affinity labelling of monoamine oxidase type B in human brain and platelet membranes. Tritium 1-3 monoamine oxidase B Homo sapiens 60-84 2222781-2 1990 With this in mind we studied platelet monoamine oxidase B (MAO B) activity and 3H-imipramine (IMI) binding in both AD patients and healthy subjects and found a significantly higher level of platelet MAO B activity and 3H-IMI Bmax values in the AD patients. Tritium 79-81 monoamine oxidase B Homo sapiens 199-204 35624746-6 2022 Statistically significant MAO-B inhibitory effects were exerted by some of the compounds where again the catecholic compound 3h was the most potent inhibitor similar to selegiline and rasagiline. Tritium 125-127 monoamine oxidase B Homo sapiens 26-31 2744079-0 1989 [3H]Ro 19-6327: a reversible ligand and affinity labelling probe for monoamine oxidase-B. Tritium 1-3 monoamine oxidase B Homo sapiens 69-88 2744079-2 1989 This compound is a novel, time-dependent inhibitor of monoamine oxidase type B (MAO-B) and is structurally closely related to [3H]Ro 16-6491. Tritium 127-129 monoamine oxidase B Homo sapiens 54-78 2744079-2 1989 This compound is a novel, time-dependent inhibitor of monoamine oxidase type B (MAO-B) and is structurally closely related to [3H]Ro 16-6491. Tritium 127-129 monoamine oxidase B Homo sapiens 80-85 2744079-5 1989 The dissociation rate was markedly decreased (t1/2 = greater than 24h) at 0 degrees C. MAO-B, but not MAO-A inhibitors, effectively prevented the binding of [3H]Ro 19-6327. Tritium 158-160 monoamine oxidase B Homo sapiens 87-92 2744079-6 1989 Like [3H]Ro 16-6491, [3H]Ro 19-6327 is recognized as a substrate by MAO-B, being eventually deaminated by the enzyme. Tritium 6-8 monoamine oxidase B Homo sapiens 68-73 2744079-6 1989 Like [3H]Ro 16-6491, [3H]Ro 19-6327 is recognized as a substrate by MAO-B, being eventually deaminated by the enzyme. Tritium 22-24 monoamine oxidase B Homo sapiens 68-73 2744079-7 1989 Since the deaminated aldehyde derivative of Ro 19-6327 did not inhibit MAO-B, a still unidentified reversible adduct, formed at the MAO-B active site, might explain the high potency and selectivity of [3H]Ro 19-6327. Tritium 202-204 monoamine oxidase B Homo sapiens 132-137 2744079-10 1989 The presence of unlabelled MAO-B inhibitors in the incubation mixture prevented the covalent incorporation of [3H]Ro 19-6327. Tritium 111-113 monoamine oxidase B Homo sapiens 27-32 2744079-11 1989 The irreversible MAO-B inhibitor, [3H] pargyline, labelled a protein with a molecular weight identical to the protein labelled by [3H]Ro 19-6327. Tritium 35-37 monoamine oxidase B Homo sapiens 17-22 3126263-5 1988 The presence of the irreversible MAO-B inhibitor l-deprenyl completely abolished the irreversible labelling of the membranes by [3H]Ro 16-6491. Tritium 129-131 monoamine oxidase B Homo sapiens 33-38 3126263-6 1988 The selective inactivation of MAO-B, e.g., by l-deprenyl prevented the covalent incorporation of [3H]Ro 16-6491 whereas selective inhibition of the MAO-A by clorgyline was without effect. Tritium 98-100 monoamine oxidase B Homo sapiens 30-35 3126263-9 1988 Our results indicate that the polypeptide that is covalently labelled by [3H]Ro 16-6491 corresponds to one of the two MAO-B subunits. Tritium 74-76 monoamine oxidase B Homo sapiens 118-123 3126263-1 1988 [3H]Ro 16-6491 [N-(2-aminoethyl)-p-chlorobenzamide HCl], a reversible "mechanism-based" inhibitor of monoamine oxidase (MAO) type B, binds selectively and with high affinity to the active site of MAO-B in brain and platelet membranes. Tritium 1-3 monoamine oxidase B Homo sapiens 101-131 3126263-1 1988 [3H]Ro 16-6491 [N-(2-aminoethyl)-p-chlorobenzamide HCl], a reversible "mechanism-based" inhibitor of monoamine oxidase (MAO) type B, binds selectively and with high affinity to the active site of MAO-B in brain and platelet membranes. Tritium 1-3 monoamine oxidase B Homo sapiens 196-201 6191193-4 1983 The concentration of immunologically detectable MAO B protein in the extracts was estimated from immunoprecipitation competition data by reference to a standard curve relating observed inhibition of immunoprecipitation to the concentration of catalytically active platelet MAO added (estimated from [3H]pargyline binding data). Tritium 300-302 monoamine oxidase B Homo sapiens 48-53 3794699-3 1987 Inhibitors and substrates of MAO-B inhibited binding of [3H]Ro 16-6491, whereas MAO-A blockers were much less potent. Tritium 57-59 monoamine oxidase B Homo sapiens 29-34 3794699-6 1987 In conclusion, [3H]Ro 16-6491 binds selectively to MAO-B and represents an excellent new radioligand probe for studying the regional tissue distribution of this enzyme in normal and pathological conditions. Tritium 16-18 monoamine oxidase B Homo sapiens 51-56 3527152-3 1986 The antibody indirectly precipitates [3H]pargyline-labelled human MAO B both from liver and platelet extracts but fails to precipitate MAO A from liver extracts. Tritium 38-40 monoamine oxidase B Homo sapiens 66-71 3566791-5 1987 When [3H]FNPA was photoirradiated with the purified MAO-B, a single radioactive band associated with MAO-B was observed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Tritium 6-8 monoamine oxidase B Homo sapiens 52-57 3566791-5 1987 When [3H]FNPA was photoirradiated with the purified MAO-B, a single radioactive band associated with MAO-B was observed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Tritium 6-8 monoamine oxidase B Homo sapiens 101-106 3566791-7 1987 Complete tryptic-chymotryptic digestion of [3H]FNPA-labeled MAO-B resulted in three radioactive peaks on Sephadex G-25 column chromatography. Tritium 44-46 monoamine oxidase B Homo sapiens 60-65 3566791-8 1987 With the same digestion and separation procedures, only one major radioactive peak was observed for the [3H]pargyline-labeled MAO-B, and its elution volume was different from that of [3H]FNPA-labeled peptides. Tritium 105-107 monoamine oxidase B Homo sapiens 126-131 3794699-0 1987 Binding of [3H]Ro 16-6491, a reversible inhibitor of monoamine oxidase type B, to human brain mitochondria and platelet membranes. Tritium 12-14 monoamine oxidase B Homo sapiens 53-77 3794699-1 1987 The reversible inhibitor of monoamine oxidase type B (MAO-B) [3H]Ro 16-6491 binds specifically and with high affinity to a single population of binding sites in human frontal cortex crude mitochondria and platelet membranes. Tritium 62-64 monoamine oxidase B Homo sapiens 28-52 3794699-1 1987 The reversible inhibitor of monoamine oxidase type B (MAO-B) [3H]Ro 16-6491 binds specifically and with high affinity to a single population of binding sites in human frontal cortex crude mitochondria and platelet membranes. Tritium 62-64 monoamine oxidase B Homo sapiens 54-59 7264664-2 1981 [3H]Pargyline was bound to MAO A in a crude mitochondrial fraction from the placental trophoblast of a male newborn and to MAO B in blood platelets from the umbilical vein of the same newborn. Tritium 1-3 monoamine oxidase B Homo sapiens 123-128 7264664-3 1981 [3H]Pargyline was also bound to MAO A and B in a crude mitochondrial fraction from cultured skin fibroblasts of a male adult and to MAO B in blood platelets from the same individual. Tritium 1-3 monoamine oxidase B Homo sapiens 132-137 7264664-5 1981 For all tissues, SDS-PAGE of [3H]pargyline-bound samples revealed a labeled protein band of apparent molecular weight 63,000 for MAO A and 60,000 for MAO B. Tritium 30-32 monoamine oxidase B Homo sapiens 150-155 23207410-4 2013 Moreover, partial recovery of MAO-B activity was observed after repeated washing in the presence of isatin (reversible inhibitor) and compounds 3g and 3h suggesting a reversible inhibition of the enzyme. Tritium 151-153 monoamine oxidase B Homo sapiens 30-35 34047146-5 2021 When enzyme inhibition activities were evaluated, it was determined that the compounds 3a (42.33%) and 3d (42.39%) on acetylcholinesterase (AChE) enzyme; compounds 3g (75.42%) and 3h (60.33%) showed inhibition on MAO-B enzyme at most, at 10-3 M concentration. Tritium 180-182 monoamine oxidase B Homo sapiens 213-218 30481645-6 2019 Also, compounds 3a (IC50 = 0.114 microM), 3h (IC50 = 0.049 microM), and 3i (IC50 = 0.054 microM) were the most active derivatives against MAO-B enzyme activity. Tritium 42-44 monoamine oxidase B Homo sapiens 138-143 19789505-3 2009 MATERIAL/METHODS: We investigated MAO labeling with mechanism-based inhibitor [3H]pargyline activities of MAO A, MAO B, and SSAO in healthy and inflamed human dental pulp. Tritium 79-81 monoamine oxidase B Homo sapiens 113-118 17570647-0 2008 Specific binding of [3H]Ro 19-6327 (lazabemide) to monoamine oxidase B is increased in frontal cortex of suicide victims after controlling for age at death. Tritium 21-23 monoamine oxidase B Homo sapiens 51-70 17570647-3 2008 We have evaluated the association of MAO-B density (assessed by [3H]Ro 19-6327 - lazabemide - binding) with type of death (suicide victims vs non-suicide controls) after controlling for age at death. Tritium 65-67 monoamine oxidase B Homo sapiens 37-42 15262334-3 2004 Utilizing [3H]BC, we have identified several proteins to which BC binds with high affinity (e.g. the chaperone member glucose regulated protein 78, the enzyme carboxylesterase, the cytochrome P450 2E1, the enzyme monoamine oxidase B and a small G-protein of the Rho subfamily). Tritium 11-13 monoamine oxidase B Homo sapiens 213-232