PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 31511328-5 2019 Pharmacological inhibition of Notch1 by gamma-secretase inhibitor DAPT (N-[N-(3,5-Difluorophenacetyl)-l-alanyl]-S-phenyl glycine t-butylester) abrogates GH-induced epithelial to mesenchymal transition (EMT) and is associated with a reduction in podocyte loss. dapt 66-70 notch 1 Mus musculus 30-36 32157565-6 2020 Two chemical drugs, 2,3-Bis(4-hydroxyphenyl)-propionitrile (DPN) and N-[N-(3,5-difluorophenacetyl)-l-alanyl]-s-phenylglycine t-butyl ester (DAPT), a specific inhibitor of Notch1 signalling) were administered via intraperitoneal injection to change oestrogen receptor beta and Notch1 activities. dapt 69-138 notch 1 Mus musculus 171-177 31347026-7 2020 Notch-1 signaling inhibition, through intrathecal administration of the gamma-secretase inhibitor, DAPT, counteracted PKC and ERK overphosphorylation, MOR internalization, and analgesic tolerance. dapt 99-103 notch 1 Mus musculus 0-7 32482162-9 2020 Presence of Notch signaling activity was confirmed through nuclear NICD and Hes1 detection, and downregulation of Hes1 transcription following canonical signaling blockade with DAPT. dapt 177-181 notch 1 Mus musculus 12-17 32482162-12 2020 Notch signaling blockade by DAPT downregulated transcription of Sox2, and retarded embryo hatching. dapt 28-32 notch 1 Mus musculus 0-5 29804421-4 2018 HE staining showed the most obvious necrosis of curcumin-PDT group with DAPT.Both DAPT and curcumin-PDT could reduce the expression level of Notch1 in mRNA.The inhibition rates were 42.17% and 40.54%, respectively.And the inhibitory effect of curcumin-PDT with DAPT on Notch-1 was the strongest (79.22%) (P<0.01), and two of them had synergistic effect after combination with curcumin-PDT.But the expression of Notch-2 has no obvious inhibitory effect (P>0.05). dapt 72-76 notch 1 Mus musculus 141-147 31417656-7 2019 mRNA and protein expression of gene Notch-1 and its downstream NF-kappaB and VEGF were observed with RT-PCR, immunohistochemical staining and Western-blot with/without inhibition of Notch signaling pathway by DAPT, both in vivo and in vitro experiments. dapt 209-213 notch 1 Mus musculus 36-43 31145652-8 2019 As Notch activity was blocked by the DAPT, the related proteins were downregulated, and the initiating cell proliferation of curcumin was abolished. dapt 37-41 notch 1 Mus musculus 3-8 30778288-10 2019 These effects are blocked by the administration of the gamma-secretase inhibitor DAPT, a specific blocker of the Notch signaling pathway. dapt 81-85 notch 1 Mus musculus 113-118 31383289-5 2019 Then, DAPT was found to inhibit Notch1ICD expression in a concentration-dependent manner, and this expression was directly correlated with Phospho-Erk1/2 (p-Erk) by using Western blotting. dapt 6-10 notch 1 Mus musculus 32-38 30539813-0 2019 Neuroprotective effect of Notch pathway inhibitor DAPT against focal cerebral ischemia/reperfusion 3 hours before model establishment. dapt 50-54 notch 1 Mus musculus 26-31 30539813-1 2019 As an inhibitor of the Notch signaling pathway, N-[N-(3,5-difluorohenacetyl)-l-alanyl]-S-phenylglycine tert-butyl ester (DAPT) may protect brain tissue from serious ischemic injury. dapt 121-125 notch 1 Mus musculus 23-28 30539813-8 2019 DAPT decreased the number of glial fibrillary acidic protein- and Notch1-positive cells in the right prefrontal cortex, while also reducing the number of apoptotic cells and decreasing interleukin-6 and tumor necrosis factor-alpha contents, and simultaneously downregulating Hes1 and Hes5 protein expression. dapt 0-4 notch 1 Mus musculus 66-72 29779621-10 2018 DAPT treatment partially rescued the elevated mRNA expression and immuno-positive cell numbers of Notch1, Jagged1 and Hes5. dapt 0-4 notch 1 Mus musculus 98-104 29779621-13 2018 Inhibiting Notch signaling activation by DAPT can partially delay the progress of TMJOA. dapt 41-45 notch 1 Mus musculus 11-16 29804421-4 2018 HE staining showed the most obvious necrosis of curcumin-PDT group with DAPT.Both DAPT and curcumin-PDT could reduce the expression level of Notch1 in mRNA.The inhibition rates were 42.17% and 40.54%, respectively.And the inhibitory effect of curcumin-PDT with DAPT on Notch-1 was the strongest (79.22%) (P<0.01), and two of them had synergistic effect after combination with curcumin-PDT.But the expression of Notch-2 has no obvious inhibitory effect (P>0.05). dapt 82-86 notch 1 Mus musculus 141-147 29804421-4 2018 HE staining showed the most obvious necrosis of curcumin-PDT group with DAPT.Both DAPT and curcumin-PDT could reduce the expression level of Notch1 in mRNA.The inhibition rates were 42.17% and 40.54%, respectively.And the inhibitory effect of curcumin-PDT with DAPT on Notch-1 was the strongest (79.22%) (P<0.01), and two of them had synergistic effect after combination with curcumin-PDT.But the expression of Notch-2 has no obvious inhibitory effect (P>0.05). dapt 82-86 notch 1 Mus musculus 269-276 29804421-4 2018 HE staining showed the most obvious necrosis of curcumin-PDT group with DAPT.Both DAPT and curcumin-PDT could reduce the expression level of Notch1 in mRNA.The inhibition rates were 42.17% and 40.54%, respectively.And the inhibitory effect of curcumin-PDT with DAPT on Notch-1 was the strongest (79.22%) (P<0.01), and two of them had synergistic effect after combination with curcumin-PDT.But the expression of Notch-2 has no obvious inhibitory effect (P>0.05). dapt 82-86 notch 1 Mus musculus 141-147 29804421-4 2018 HE staining showed the most obvious necrosis of curcumin-PDT group with DAPT.Both DAPT and curcumin-PDT could reduce the expression level of Notch1 in mRNA.The inhibition rates were 42.17% and 40.54%, respectively.And the inhibitory effect of curcumin-PDT with DAPT on Notch-1 was the strongest (79.22%) (P<0.01), and two of them had synergistic effect after combination with curcumin-PDT.But the expression of Notch-2 has no obvious inhibitory effect (P>0.05). dapt 82-86 notch 1 Mus musculus 269-276 29804421-5 2018 Both DAPT and curcumin-PDT can inhibit the protein expression of Notch1, NF-kappaB and VEGF, and two of them have synergistic effect after combined use. dapt 5-9 notch 1 Mus musculus 65-71 29804421-6 2018 Conclusions: DAPT can effectively block the Notch signaling pathway and inhibit the proliferation of cervical cancer cell line Me180.The application of DAPT to inhibit Notch signaling pathway after photodynamic therapy can achieve synergistic effect, which is mainly related to the down-regulation of the expression of Notch1 and NF-kappaB.Notch signaling pathway may be one of the targets of curcumin-PDT photodynamic therapy. dapt 13-17 notch 1 Mus musculus 319-325 29804421-6 2018 Conclusions: DAPT can effectively block the Notch signaling pathway and inhibit the proliferation of cervical cancer cell line Me180.The application of DAPT to inhibit Notch signaling pathway after photodynamic therapy can achieve synergistic effect, which is mainly related to the down-regulation of the expression of Notch1 and NF-kappaB.Notch signaling pathway may be one of the targets of curcumin-PDT photodynamic therapy. dapt 152-156 notch 1 Mus musculus 319-325 28158917-7 2017 Further, to better understand the relationship between Notch signalling and autophagy in podocyte differentiation, rapamycin was added to enhance autophagy levels in DAPT-treated cells, and as a result, nephrin was recovered and DAPT-induced injury was ameliorated. dapt 166-170 notch 1 Mus musculus 55-60 29523162-12 2018 Data obtained from in vitro study in IMQ-treated mice also demonstrated that blocking Notch1 signaling by DAPT can result in a dose-dependent decrease of Th17 cell proportion, mRNA expression of Notch1, Hes-1, RORgammat and IL-17A as well as IL-17A secretion in splenic CD4+ T cells. dapt 106-110 notch 1 Mus musculus 86-92 29523162-12 2018 Data obtained from in vitro study in IMQ-treated mice also demonstrated that blocking Notch1 signaling by DAPT can result in a dose-dependent decrease of Th17 cell proportion, mRNA expression of Notch1, Hes-1, RORgammat and IL-17A as well as IL-17A secretion in splenic CD4+ T cells. dapt 106-110 notch 1 Mus musculus 195-201 29523162-13 2018 CONCLUSION: These data suggest that Notch1 inhibition by DAPT can effectively alleviate the severity of mouse psoriasis-like skin inflammation by regulating the differentiation and function of Th17 cells, indicating that DAPT might be a potential therapeutic candidate for the treatment of psoriatic inflammation. dapt 57-61 notch 1 Mus musculus 36-42 29523162-13 2018 CONCLUSION: These data suggest that Notch1 inhibition by DAPT can effectively alleviate the severity of mouse psoriasis-like skin inflammation by regulating the differentiation and function of Th17 cells, indicating that DAPT might be a potential therapeutic candidate for the treatment of psoriatic inflammation. dapt 221-225 notch 1 Mus musculus 36-42 28823624-9 2018 However, when Notch activity was blocked by the gamma-secretase inhibitor DAPT, the expression of Notch 1 and Hes 1 mRNA was down-regulated, augmentation of NICD and Hes 1 protein was ameliorated, the proliferation-inducing effect of Ost was abolished. dapt 74-78 notch 1 Mus musculus 14-19 28823624-9 2018 However, when Notch activity was blocked by the gamma-secretase inhibitor DAPT, the expression of Notch 1 and Hes 1 mRNA was down-regulated, augmentation of NICD and Hes 1 protein was ameliorated, the proliferation-inducing effect of Ost was abolished. dapt 74-78 notch 1 Mus musculus 98-105 29169413-3 2017 Prior to stimulation with LPS, mouse RAW264.7 cells were treated with DAPT (10 mumol/L), an inhibitor of Notch1 signaling, for 1 hour. dapt 70-74 notch 1 Mus musculus 105-111 27168786-9 2016 The inhibition of Notch by DAPT resulted in fewer EdU-positive cells and the upregulation of the expression levels of various mucous cell-associated genes. dapt 27-31 notch 1 Mus musculus 18-23 28286920-6 2017 Indeed, paracetamol/acetaminophen-induced liver damage was worse after Notch blockade with DAPT in wild-type mice, which was accompanied by significantly increased ALT levels, diminished hairy and enhancer of split-1 (Hes1), and phosphorylated Stat3 and Akt but enhanced high mobility group box 1 (HMGB1), TLR4, NF-kappaB, and NLRP3 activation after APAP challenge. dapt 91-95 notch 1 Mus musculus 71-76 28112174-8 2017 Moreover, the protective effects conferred by TSG on SIR-treated H9c2 cardiomyoblasts were abolished by co-administration of DAPT (the Notch1 signaling inhibitor). dapt 125-129 notch 1 Mus musculus 135-141 28904317-5 2017 In the presence of DAPT, a Notch signaling inhibitor, during osteogenic induction, mRNA levels for osteogenic marker genes were significantly decreased; however, no difference was noted in mineral deposition. dapt 19-23 notch 1 Mus musculus 27-32 26825631-4 2016 Here, we evaluated the transcription and expression patterns of Notch components (Notch1-3, Dll1, Dll4, and Jagged1) and effectors (Hes1-2 and Hes5) in the adult mouse epididymis, and evaluated the role of Notch signaling in the epididymis through its in vivo blockade following administration of an inhibitor (DAPT). dapt 311-315 notch 1 Mus musculus 64-69 26825631-11 2016 DAPT-induced in vivo Notch signaling blockade, although showing a low efficiency, disrupted the expression patterns of Notch components and effectors in the epididymal epithelium and in spermatozoa, and significantly decreased sperm motility, although not affecting male fertility. dapt 0-4 notch 1 Mus musculus 21-26 26573194-9 2015 DAPT treatment led to a decrease above the index serum levels of HMGB1 (6.22+/-0.71) and IL-10 (252.06+/-57.63), and of expression of Notch 1 (mRNA: 3.20+/-0.68), NICD (protein: 0.42+/-0.05), and Hes5 (mRNA: 4.72+/-0.67; protein: 0.84+/-0.09) (P<0.01 or <0.05). dapt 0-4 notch 1 Mus musculus 134-141 24573392-6 2014 In wild-type mice pharmacological inhibition of Notch using the gamma-secretase inhibitor N-[N-(3,5-difluorophenacetyl)-l-alanyl]-S-phenylglycine t-butyl ester (DAPT) improved tubulo-interstitial damage and antagonized the prosenescent pathway activation after IR. dapt 90-159 notch 1 Mus musculus 48-53 26328484-7 2015 However, when Notch activity was blocked by the gamma-secretase inhibitor DAPT, the augmentation of Notch 1 and Hes 1 protein was ameliorated, and the proliferation-inducing effect of osthole was abolished, suggesting that the effects of osthole are at least in part mediated by activation of the Notch pathway. dapt 74-78 notch 1 Mus musculus 14-19 26328484-7 2015 However, when Notch activity was blocked by the gamma-secretase inhibitor DAPT, the augmentation of Notch 1 and Hes 1 protein was ameliorated, and the proliferation-inducing effect of osthole was abolished, suggesting that the effects of osthole are at least in part mediated by activation of the Notch pathway. dapt 74-78 notch 1 Mus musculus 100-107 26328484-7 2015 However, when Notch activity was blocked by the gamma-secretase inhibitor DAPT, the augmentation of Notch 1 and Hes 1 protein was ameliorated, and the proliferation-inducing effect of osthole was abolished, suggesting that the effects of osthole are at least in part mediated by activation of the Notch pathway. dapt 74-78 notch 1 Mus musculus 100-105 24573392-6 2014 In wild-type mice pharmacological inhibition of Notch using the gamma-secretase inhibitor N-[N-(3,5-difluorophenacetyl)-l-alanyl]-S-phenylglycine t-butyl ester (DAPT) improved tubulo-interstitial damage and antagonized the prosenescent pathway activation after IR. dapt 161-165 notch 1 Mus musculus 48-53 23704950-8 2013 Moreover, pharmacological inhibition of endogenous Notch-1 signaling by N-3,5-difluorophenyl acetyl-L-alanyl-2-phenylglycine-1,1-dimethylethyl ester (DAPT), a gamma-secretase specific inhibitor, as well as the silencing of Notch-1 ligand, Jagged-1, potentiated TGF-beta1-induced myofibroblast differentiation and abrogated the inhibitory effects of RLX. dapt 150-154 notch 1 Mus musculus 51-58 24430295-3 2014 The receptor and ligand genes of Notch pathway (Notch1, Notch2, Jagged1, Jagged2 and Hes1) were extremely down-regulated after newborn mouse ovaries were cultured then exposed to DAPT or L-685,458 in vitro (P < 0.01). dapt 179-183 notch 1 Mus musculus 33-38 24430295-3 2014 The receptor and ligand genes of Notch pathway (Notch1, Notch2, Jagged1, Jagged2 and Hes1) were extremely down-regulated after newborn mouse ovaries were cultured then exposed to DAPT or L-685,458 in vitro (P < 0.01). dapt 179-183 notch 1 Mus musculus 48-54 24430295-4 2014 Since DAPT or L-685,548 inhibits Notch signaling pathway, the expression of protein LHX8 and NOBOX was significantly reduced during the formation of the primordial follicles. dapt 6-10 notch 1 Mus musculus 33-38 24197755-10 2014 The inhibition of notch signaling activity by DAPT significantly attenuated the isoflurane preconditioning-induced neuroprotection, and similar results were obtained using notch knockout mice. dapt 46-50 notch 1 Mus musculus 18-23 20447060-9 2010 CONCLUSIONS: We conclude that blocking Notch signalling with DAPT enhances adipogenesis of differentiated mASCs at an early stage. dapt 61-65 notch 1 Mus musculus 39-44 23188126-10 2013 All these curative effects were suppressed by pharmacological blockade of Notch signaling with DAPT. dapt 95-99 notch 1 Mus musculus 74-79 23028940-7 2012 Inhibition of endothelial cell growth and sprouting angiogenesis by DLL4/Notch signaling was enhanced in SIRT1-silenced lung cancer-derived EC and rescued by Notch inhibitor DAPT. dapt 174-178 notch 1 Mus musculus 73-78 23028940-7 2012 Inhibition of endothelial cell growth and sprouting angiogenesis by DLL4/Notch signaling was enhanced in SIRT1-silenced lung cancer-derived EC and rescued by Notch inhibitor DAPT. dapt 174-178 notch 1 Mus musculus 158-163 21723221-8 2011 RESULTS: Wild-type mice were more susceptible to CAC following inhibition of Notch1 by DAPT, shown by increased numbers of tumors and level of dysplasia compared with controls. dapt 87-91 notch 1 Mus musculus 77-83 21880906-11 2011 Specificity of Notch1 signaling in vivo was validated in mice exposed to DAPT, which failed to demonstrate barrier disruption following cocaine exposure. dapt 73-77 notch 1 Mus musculus 15-21 20692087-5 2010 DAPT inhibited the expression of Notch target gene Hes1 in the cervical loop indicating that Notch signalling was inhibited in the putative stem cells. dapt 0-4 notch 1 Mus musculus 33-38 20692087-5 2010 DAPT inhibited the expression of Notch target gene Hes1 in the cervical loop indicating that Notch signalling was inhibited in the putative stem cells. dapt 0-4 notch 1 Mus musculus 93-98 20648656-0 2010 Nuclear factor-kappaB/p65 responds to changes in the Notch signaling pathway in murine BV-2 cells and in amoeboid microglia in postnatal rats treated with the gamma-secretase complex blocker DAPT. dapt 191-195 notch 1 Mus musculus 53-58 20648656-5 2010 In BV-2 cells pretreated with N-[N-(3,5-difluorophenacetyl)-1-alany1]-S-phenyglycine t-butyl ester (DAPT), a gamma-secretase inhibitor, followed by LPS stimulation, Notch-1 expression level was enhanced but that of all other markers was suppressed. dapt 100-104 notch 1 Mus musculus 165-172 21312186-5 2011 In the mouse model of bleomycin-induced dermal fibrosis and in tight skin 1 mice, Notch signaling was inhibited by the gamma-secretase inhibitor DAPT and by overexpression of a Notch-1 antisense construct. dapt 145-149 notch 1 Mus musculus 82-87 35489123-5 2022 The upregulation of Notch1, Notch1 intracellular domain (NICD), and Hes1 proteins by LPS treatment was inhibited by DAPT, a Notch1 inhibitor. dapt 116-120 notch 1 Mus musculus 20-26 19706332-7 2009 The role of the Notch signaling pathway analyzed with the specific inhibitor, DAPT, and by examining the expression of Notch-related genes using RT-PCR showed that after co-culturing with MSCs for 24h, NSCs expressed much higher levels of ki-67, Notch1, and Hes1 than did NSCs co-cultured with NIH3T3 cells. dapt 78-82 notch 1 Mus musculus 16-21 19706332-8 2009 Treatment with DAPT decreased ki-67, Notch1 and Hes1 expression in NCSs, and increased Mash1 expression. dapt 15-19 notch 1 Mus musculus 37-43 16959876-10 2006 Treatment with the gamma-secretase inhibitor DAPT down-regulated Notch activity and reduced the proportion of SP cells. dapt 45-49 notch 1 Mus musculus 65-70 12952904-4 2003 We therefore cultured mouse metanephroi in the presence of a gamma-secretase inhibitor, N-S-phenyl-glycine-t-butyl ester (DAPT), to block Notch signaling. dapt 122-126 notch 1 Mus musculus 138-143 34320265-10 2021 At last, as a Notch signaling inhibitor, the gamma-secretase inhibitor N-(N-(3,5-difl uorophenacetyl)-L-alanyl)-S-phenylglycine t-butyl ester (DAPT) pretreatment could alleviate the expression of collagens and the symptoms of fibrosis. dapt 143-147 notch 1 Mus musculus 14-19 17922104-7 2007 Inhibiting Notch signalling with N-[N-(3,5-difluorophenacetyl-L-alanyl)]-S-phenylglycine t-butyl ester (DAPT) increased Ngn3 mRNA expression and protein levels in adult islets. dapt 104-108 notch 1 Mus musculus 11-16 35489123-5 2022 The upregulation of Notch1, Notch1 intracellular domain (NICD), and Hes1 proteins by LPS treatment was inhibited by DAPT, a Notch1 inhibitor. dapt 116-120 notch 1 Mus musculus 124-130 35489123-7 2022 Furthermore, suppression of Notch signaling by DAPT upregulated Cylindromatosis (CYLD) expression but downregulated TRAF6 expression, IkappaB kinase (IKK) alpha/beta phosphorylation, and subsequently, phosphorylation and degradation of IkappaB-alpha, indicating that DAPT inhibited NF-kappaB activation triggered by TLR-4. dapt 47-51 notch 1 Mus musculus 28-33 35489123-7 2022 Furthermore, suppression of Notch signaling by DAPT upregulated Cylindromatosis (CYLD) expression but downregulated TRAF6 expression, IkappaB kinase (IKK) alpha/beta phosphorylation, and subsequently, phosphorylation and degradation of IkappaB-alpha, indicating that DAPT inhibited NF-kappaB activation triggered by TLR-4. dapt 267-271 notch 1 Mus musculus 28-33 35489123-5 2022 The upregulation of Notch1, Notch1 intracellular domain (NICD), and Hes1 proteins by LPS treatment was inhibited by DAPT, a Notch1 inhibitor. dapt 116-120 notch 1 Mus musculus 28-34