PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 9593987-4 1998 We made use of enhanced green fluorescence protein (EGFP), a reporter protein, to follow redistribution of Phd both by means of confocal microscopy and biochemical techniques in living neuronal NG 108-15 hybrid cells challenged with prostaglandin E1 (PGE1). Alprostadil 233-249 phosducin Mus musculus 107-110 9593987-4 1998 We made use of enhanced green fluorescence protein (EGFP), a reporter protein, to follow redistribution of Phd both by means of confocal microscopy and biochemical techniques in living neuronal NG 108-15 hybrid cells challenged with prostaglandin E1 (PGE1). Alprostadil 251-255 phosducin Mus musculus 107-110 9593987-11 1998 The present study reports for living NG 108-15 hybrid cells that PGE1 stimulation causes cytosolic Phd to translocate towards the membrane, where it is believed to bind to G protein subunits such as Gbetagamma and Galphas. Alprostadil 65-69 phosducin Mus musculus 99-102 9496706-0 1998 Phosducin expression in NG 108-15 hybrid cells enhances prostaglandin E1 stimulated adenylate cyclase activity. Alprostadil 56-72 phosducin Mus musculus 0-9 9496706-4 1998 The neuronal cells stably expressing Phd were found to display a 3 to 4-fold increased sensitivity to PGE1 as compared to wild type cells, using cAMP accumulation as measure. Alprostadil 102-106 phosducin Mus musculus 37-40 9496706-5 1998 Examination of membranes prepared from Phd-overexpressing cells revealed an elevated GTPase activity as indicated by the formation of 32Pi upon PGE1 challenge. Alprostadil 144-148 phosducin Mus musculus 39-42 9496706-8 1998 The findings let us propose that Phd elevates intracellular levels of functional G alpha s which accounts for the increased response to PGE1. Alprostadil 136-140 phosducin Mus musculus 33-36