PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 30286548-10 2018 Identified 386 genes which exhibited both differential methylation and expression were functionally associated with pathways including cardiovascular system development, regulation of blood vessel size, vasculature development, p53 pathway, AC-modulating/inhibiting GPCRs pathway and cellular response to metal ion/inorganic substance. Metals 305-310 tumor protein p53 Homo sapiens 228-231 34028595-6 2021 In turn, metal-induced inhibition of SIRT was shown to affect deacetylation of target proteins including FOXO, PGC1alpha, p53 and NF-kB. Metals 9-14 tumor protein p53 Homo sapiens 122-125 33317179-3 2020 METHOD: We conjugated p53-specific antibodies to metal tags for detection by mass cytometry, allowing the detection of proteins and their post-translational modifications in single cells. Metals 49-54 tumor protein p53 Homo sapiens 22-25 33538587-1 2021 We identified a set of thiosemicarbazone (TSC) metal ion chelators that reactivate specific zinc-deficient p53 mutants using a mechanism called zinc metallochaperones (ZMCs) that restore zinc binding by shuttling zinc into cells. Metals 47-52 tumor protein p53 Homo sapiens 107-110 32976933-5 2020 These groups also exhibited positive correlations between high metal concentrations and the average p53 expression levels, but negative correlations with the mean p-Akt cascade protein levels in sperm cells. Metals 63-68 tumor protein p53 Homo sapiens 100-103 32976933-7 2020 Therefore, our findings may suggest that graded levels of metal exposure significantly influence the relative fluctuation in the levels of p53 and Akt cascade proteins in the sperm cells of infertile subjects. Metals 58-63 tumor protein p53 Homo sapiens 139-142 32138726-8 2020 CONCLUSION: MDA,8-OHdG and wildtype p53 can be used as genotoxic biomarkers in the metal exposed group, since they can accurately reflect the degree of Oxidative damage. Metals 83-88 tumor protein p53 Homo sapiens 36-39 30345409-9 2017 The putative toxicity pathway for cytotoxicity of these metals and metal mixtures identified by LASSO is composed of phospho-RPS6KB1, phospho-p53, cleaved CASP3, phospho-MAPK8, IL-10, and Hif-1alpha. Metals 56-61 tumor protein p53 Homo sapiens 142-145 28754018-2 2017 Metal ions, such as magnesium and zinc ions, have important influences on p53-DNA interactions for stabilizing the structure of the protein and enhancing its affinity to DNA. Metals 0-5 tumor protein p53 Homo sapiens 74-77 28754018-3 2017 In the present study, we systematically investigated the interaction of full length human protein p53 with DNA in metal ion solution by atomic force microscopy (AFM). Metals 114-119 tumor protein p53 Homo sapiens 98-101 20875833-5 2011 These results support a model for copper-responsive transcription in which the metal affects ATF3 expression and stabilizes p53 resulting in the down-regulation of HNF4alpha expression. Metals 79-84 tumor protein p53 Homo sapiens 124-127 26391003-1 2015 This work describes cytotoxic effect of non-platinum metal-based compounds on the human T-leukemic cells with different p53 status (p53 wild-type MOLT-4 and p53-deficient Jurkat cells). Metals 53-58 tumor protein p53 Homo sapiens 132-135 26391003-1 2015 This work describes cytotoxic effect of non-platinum metal-based compounds on the human T-leukemic cells with different p53 status (p53 wild-type MOLT-4 and p53-deficient Jurkat cells). Metals 53-58 tumor protein p53 Homo sapiens 132-135 25710967-2 2015 The most common mutation, R175H, inactivates p53 by reducing its affinity for the essential zinc ion, leaving the mutant protein unable to bind the metal in the low [Zn(2+)]free environment of the cell. Metals 148-153 tumor protein p53 Homo sapiens 45-48 25294809-6 2014 The pharmacologic delivery of a metal ion to restore proper folding of a mutant protein is unique to medicinal chemistry and represents a new pathway to drug mutant p53. Metals 32-37 tumor protein p53 Homo sapiens 165-168 23201157-5 2013 EXPERIMENTAL DESIGN: In vitro phosphorylation of p53 by Aurora-A was performed and the phosphorylated protein was then digested with trypsin and enriched for phosphopeptides by immobilized metal affinity chromatography. Metals 189-194 tumor protein p53 Homo sapiens 49-52 27390612-4 2016 This chimeric protein, M3-p53-R12, can be expressed in E. coli and purified using immobilized metal ion chromatography followed by serial refolding dialysis. Metals 94-99 tumor protein p53 Homo sapiens 26-29 25563581-8 2015 The Gene Ontology and KEGG pathway analysis revealed that the target genes of co-expressed miRNAs were highly involved in the cell cycle process, metal ion binding, modification of plasma membrane, and the p53 signal pathway. Metals 146-151 tumor protein p53 Homo sapiens 206-209 21194611-1 2011 The p53 tumor suppressor protein is one of the key checkpoints in cellular response to a variety of stress mechanisms, including exposure to various toxic metal complexes. Metals 155-160 tumor protein p53 Homo sapiens 4-7 21072344-5 2010 The zinc-binding status of p53 in the cell is impacted significantly by the presence of tumorigenic mutations and by metal ion homeostasis. Metals 117-122 tumor protein p53 Homo sapiens 27-30 18547707-9 2008 p53 and Bax mRNA are also upregulated by the metal. Metals 45-50 tumor protein p53 Homo sapiens 0-3 19346293-0 2009 Effect of metal ion on the structural stability of tumour suppressor protein p53 DNA-binding domain. Metals 10-15 tumor protein p53 Homo sapiens 77-80 19346293-6 2009 The thermal stability monitored by DSC scans showed that the binding of metal ions to p53DBD increased the thermal stability of the protein. Metals 72-77 tumor protein p53 Homo sapiens 86-89 19346293-9 2009 Analysis of acrylamide quenching experiments revealed that the binding of metal ions to p53DBD induced a structural modification of the protein and this change provided significant protection against acrylamide quenching. Metals 74-79 tumor protein p53 Homo sapiens 88-91 19346293-10 2009 Overall, the present results indicated that p53DBD underwent a conformational change upon the binding of metal ions, which was characterized by an increased stability of the protein. Metals 105-110 tumor protein p53 Homo sapiens 44-47 19655389-4 2009 By regulating metal ions, MT-I+II can control metal-containing transcription factors, zinc-finger proteins and p53. Metals 14-19 tumor protein p53 Homo sapiens 111-114 15827887-7 2005 This strong immunoreactivity of proapototic proteins (p53 and BAX) in hippocampal cultures exposed to anoxia or/and TPEN correlated with previous ultrastructural evidences of anoxia- and TPEN-induced apoptosis, while the overexpression of anti-apoptotic protein (BCL-2 and BCL-X) in zinc-pretreated cultures evidenced the protective ability of this metal against apoptosis in model of anoxia in vitro. Metals 349-354 tumor protein p53 Homo sapiens 54-57 15880691-13 2005 However, metal-induced activation of p53 and mitochondrial depolarization appears to be independent of ERK. Metals 9-14 tumor protein p53 Homo sapiens 37-40 17477370-10 2007 The above results demonstrate that activation of p53 is an important factor in metal regulation of MT. Metals 79-84 tumor protein p53 Homo sapiens 49-52 16007197-0 2005 Quantitation of p53 nuclear relocation in response to stress using a yeast functional assay: effects of irradiation and modulation by heavy metal ions. Metals 140-145 tumor protein p53 Homo sapiens 16-19 16007197-7 2005 Cd2+ and Hg2+, two metal ions inducing DNA damage as well as conformational changes in p53, have opposite effects on p53 relocation in response to DNA damage. Metals 19-24 tumor protein p53 Homo sapiens 117-120 15711927-1 2005 Previous studies revealed that cells may differ in their response to metal stress depending on their p53 status; however, the sequence of events leading to copper-induced apoptosis is still unclear. Metals 69-74 tumor protein p53 Homo sapiens 101-104 15001399-2 2004 This study was undertaken to investigate the role of PI3K/Akt signaling pathway in metal resistance in human breast cancer epithelial cells with different p53 and estrogen receptor status. Metals 83-88 tumor protein p53 Homo sapiens 155-158 15001399-7 2004 In MCF-7 cells, the metal treatment increased the phosphorylation of p53 at serine 15, up-regulated p21 expression, and resulted in cell-cycle arrest in G1 phase with apoptosis. Metals 20-25 tumor protein p53 Homo sapiens 69-72 11554448-3 2001 p53 is a zinc-binding protein containing several reactive cysteines, and its key biochemical property, sequence-specific DNA binding, is dependent upon metal and redox regulation in vitro. Metals 152-157 tumor protein p53 Homo sapiens 0-3 11554448-4 2001 In this review, we describe the main features of p53 as a metalloprotein and we discuss how metal binding and oxidation-reduction may affect p53 activity in vivo. Metals 58-63 tumor protein p53 Homo sapiens 49-52 11554448-8 2001 In this network, p53 can control the timely production of reactive oxygen intermediates (e.g., to initiate apoptosis), but this activity is itself under the control of changes in metal levels and in cellular redox status. Metals 179-184 tumor protein p53 Homo sapiens 17-20 11077439-0 2000 Metalloregulation of the tumor suppressor protein p53: zinc mediates the renaturation of p53 after exposure to metal chelators in vitro and in intact cells. Metals 111-116 tumor protein p53 Homo sapiens 50-53 11697035-1 2001 The mechanism of metal-mediated DNA damage by carcinogenic danthron (1,8-dihydroxyanthraquinone) and anthraquinone was investigated by the DNA sequencing technique using 32P-labeled human DNA fragments obtained from the human c-Ha-ras-1 protooncogene and the p53 tumor suppressor gene. Metals 17-22 tumor protein p53 Homo sapiens 259-262 11077439-0 2000 Metalloregulation of the tumor suppressor protein p53: zinc mediates the renaturation of p53 after exposure to metal chelators in vitro and in intact cells. Metals 111-116 tumor protein p53 Homo sapiens 89-92 10998350-3 2000 Treatment of MCF7 breast cancer cells with pyrrolidine dithiocarbamate (PDTC), a metal chelator, resulted in a minimum of 25% oxidation of p53. Metals 81-86 tumor protein p53 Homo sapiens 139-142 10942736-12 2000 Deferoxamine, a metal chelator, inhibited p53 activation by chelating Cr(V) to make it incapable of generating radicals from H(2)O(2). Metals 16-21 tumor protein p53 Homo sapiens 42-45 7841034-3 1995 In vitro, the conformation and DNA-binding activity of wild-type p53 are subject to redox modulation and are abrogated by exposure to metal chelators. Metals 134-139 tumor protein p53 Homo sapiens 65-68 8605209-1 1996 Oxidative DNA damage by NAD(P)H in the presence of metal ions has been characterized by using 32P 5" end-labeled DNA fragments obtained from human p53 tumor suppressor gene and c-Ha-ras-1 protooncogene. Metals 51-56 tumor protein p53 Homo sapiens 147-150 22607421-0 2000 Effects of Oxidation Agents and Metal Ions on Binding of p53 to Supercoiled DNA. Metals 32-37 tumor protein p53 Homo sapiens 57-60 10604188-0 1999 Metal ions as regulators of the conformation and function of the tumour suppressor protein p53: implications for carcinogenesis. Metals 0-5 tumor protein p53 Homo sapiens 91-94 10604188-3 1999 The p53 protein is a metal-binding transcription factor that is inactivated by metal chelation and by oxidation in vitro. Metals 21-26 tumor protein p53 Homo sapiens 4-7 10604188-3 1999 The p53 protein is a metal-binding transcription factor that is inactivated by metal chelation and by oxidation in vitro. Metals 79-84 tumor protein p53 Homo sapiens 4-7 10604188-4 1999 In intact cells, p53 protein activity is crucially dependent on the availability of Zn ions and is impaired by exposure to Cd, a metal which readily substitutes for Zn in a number of transcription factors. Metals 129-134 tumor protein p53 Homo sapiens 17-20 10604188-6 1999 Overall, these findings indicate that regulation by metals plays an important role in the control of p53, and that perturbation of this control may explain the carcinogenic potential of several metal compounds. Metals 52-57 tumor protein p53 Homo sapiens 101-104 9315628-0 1997 Regulation of p53 by metal ions and by antioxidants: dithiocarbamate down-regulates p53 DNA-binding activity by increasing the intracellular level of copper. Metals 21-26 tumor protein p53 Homo sapiens 14-17 8617769-0 1996 Modification of an N-terminal regulatory domain of T antigen restores p53-T antigen complex formation in the absence of an essential metal ion cofactor. Metals 133-138 tumor protein p53 Homo sapiens 70-73 7790313-3 1995 Immunostaining revealed nuclear accumulation of p53 within 6 h after various stresses [heat shock, osmotic shock, heavy metal (Cd), blockers of the cellular respiratory system (NaN3), amino acid analogues (azetidine and canavanine), an inhibitor of protein synthesis (puromycin), and oxygen free radicals (H2O2)]. Metals 120-125 tumor protein p53 Homo sapiens 48-51 7841034-6 1995 Destabilisation of p53 tertiary structure induced protein aggregation through hydrophobic interactions, consistent with the notion that wild-type p53 contains a hydrophobic core which may become exposed by metal chelation. Metals 206-211 tumor protein p53 Homo sapiens 19-22 7841034-6 1995 Destabilisation of p53 tertiary structure induced protein aggregation through hydrophobic interactions, consistent with the notion that wild-type p53 contains a hydrophobic core which may become exposed by metal chelation. Metals 206-211 tumor protein p53 Homo sapiens 146-149 8168507-7 1994 Using metal affinity chromatography, we have established that pure p53 binds the immobilised divalent ions Zn2+, Ni2+ and Co2+ with high affinity. Metals 6-11 tumor protein p53 Homo sapiens 67-70 7811703-0 1994 Divalent metal ions induce conformational change in pure, human wild-type p53 tumor suppressor protein. Metals 9-14 tumor protein p53 Homo sapiens 74-77 8221671-5 1993 The DNA-binding activity of wild-type p53 hybrid protein was inhibited by the metal chelator 1,10-phenanthroline. Metals 78-83 tumor protein p53 Homo sapiens 38-41 8221671-7 1993 Furthermore, metal ions may regulate binding of p53 to DNA by modulating its conformation. Metals 13-18 tumor protein p53 Homo sapiens 48-51 8467489-0 1993 A structural role for metal ions in the "wild-type" conformation of the tumor suppressor protein p53. Metals 22-27 tumor protein p53 Homo sapiens 97-100 8402615-2 1993 Here we show that both conformation and sequence-specific DNA binding of p53 translated in vitro can be modulated by metal chelators and oxidizing agents. Metals 117-122 tumor protein p53 Homo sapiens 73-76 8467489-2 1993 Here we show that exposure to the metal chelator 1,10-phenanthroline induces wild-type p53 to adopt the mutant conformation and that this process is reversible. Metals 34-39 tumor protein p53 Homo sapiens 87-90 8467489-4 1993 We propose that binding of metal ions, most probably zinc, to conserved cysteinyl residues stabilizes the tertiary structure of wild-type p53. Metals 27-32 tumor protein p53 Homo sapiens 138-141 34163734-1 2021 Dye-loaded UiO-66 metal-organic framework nanoparticles (NMOFs) modified with catalytic hemin/G-quadruplex DNAzyme labels act as functional hybrid modules for the chemiluminescence resonance energy transfer (CRET) analysis of miRNAs (miRNA-155 or miRNA-21) or genes (p53 or BRCA1). Metals 18-23 tumor protein p53 Homo sapiens 267-270 34786438-2 2021 This interplay is particularly evident in zinc-binding transcription factors such as the p53 tumor suppressor, whose DNA-binding activity can critically depend on levels of intracellular zinc as well as point mutations that alter either metal binding or folding stability. Metals 237-242 tumor protein p53 Homo sapiens 89-92 35513212-3 2022 Here, we constructed a self-assembled metal-organic nanomedicine p53/Ce6@ZF-T, which was composed of p53 plasmid-complexed chlorin e6 (Ce6)-poly(amidoamine), Fe2+-containing mesoporous zeolitic imidazolate framework-8 and naturally derived tannic acid (TA). Metals 38-43 tumor protein p53 Homo sapiens 65-68 35487360-11 2022 The metal ions with weak hydrolysis constants and strong polarization forces could readily interact with N-containing histidine and S-containing cysteine of p53 DBD, which resulted in high Ka values. Metals 4-9 tumor protein p53 Homo sapiens 157-160 35487360-12 2022 This study identified p53 as potential target for metal ions, revealed the key characteristics affecting the actions and provide a basic understanding of metal ions-p53 DBD interaction. Metals 50-55 tumor protein p53 Homo sapiens 22-25 35487360-12 2022 This study identified p53 as potential target for metal ions, revealed the key characteristics affecting the actions and provide a basic understanding of metal ions-p53 DBD interaction. Metals 154-159 tumor protein p53 Homo sapiens 22-25 35487360-12 2022 This study identified p53 as potential target for metal ions, revealed the key characteristics affecting the actions and provide a basic understanding of metal ions-p53 DBD interaction. Metals 154-159 tumor protein p53 Homo sapiens 165-168 35487360-6 2022 Some metal ions could bind to p53 core domain, impair its function and induce the development of cancer risk, but its mechanisms were still unclear. Metals 5-10 tumor protein p53 Homo sapiens 30-33 35487360-7 2022 Therefore, a quantitative structure-activity relationship (QSAR) model was constructed to characterize the binding constants (Ka) between DNA binding domain of p53 (p53 DBD) and nine metal ions (Mg2+, Ca2+, Cu2+, Zn2+, Co2+, Ni2+, Mn2+, Fe3+ and Ba2+). Metals 183-188 tumor protein p53 Homo sapiens 160-163 35487360-7 2022 Therefore, a quantitative structure-activity relationship (QSAR) model was constructed to characterize the binding constants (Ka) between DNA binding domain of p53 (p53 DBD) and nine metal ions (Mg2+, Ca2+, Cu2+, Zn2+, Co2+, Ni2+, Mn2+, Fe3+ and Ba2+). Metals 183-188 tumor protein p53 Homo sapiens 165-168 35513212-3 2022 Here, we constructed a self-assembled metal-organic nanomedicine p53/Ce6@ZF-T, which was composed of p53 plasmid-complexed chlorin e6 (Ce6)-poly(amidoamine), Fe2+-containing mesoporous zeolitic imidazolate framework-8 and naturally derived tannic acid (TA). Metals 38-43 tumor protein p53 Homo sapiens 101-104