PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 35387233-8 2022 We then demonstrated that this cofactor limitation may be partially recovered by disrupting iron homeostasis with a fra2 mutation, thereby increasing cellular iron levels. Iron 92-96 Bol2p Saccharomyces cerevisiae S288C 116-120 35387233-8 2022 We then demonstrated that this cofactor limitation may be partially recovered by disrupting iron homeostasis with a fra2 mutation, thereby increasing cellular iron levels. Iron 159-163 Bol2p Saccharomyces cerevisiae S288C 116-120 29524511-1 2018 The yeast Saccharomyces cerevisiae monothiol glutaredoxin Grx3 plays a key role in cellular defense against oxidative stress and more importantly, cooperates with BolA-like iron repressor of activation protein Fra2 to regulate the localization of the iron-sensing transcription factor Aft2. Iron 173-177 Bol2p Saccharomyces cerevisiae S288C 210-214 29524511-1 2018 The yeast Saccharomyces cerevisiae monothiol glutaredoxin Grx3 plays a key role in cellular defense against oxidative stress and more importantly, cooperates with BolA-like iron repressor of activation protein Fra2 to regulate the localization of the iron-sensing transcription factor Aft2. Iron 251-255 Bol2p Saccharomyces cerevisiae S288C 210-214 29524511-2 2018 The interplay among Grx3, Fra2 and Aft2 responsible for the regulation of iron homeostasis has not been clearly described. Iron 74-78 Bol2p Saccharomyces cerevisiae S288C 26-30 29524511-5 2018 NMR titration and pull-down assays combined with surface plasmon resonance experiments revealed that Fra2 could form a noncovalent heterodimer with Grx3 via an interface between the helix-turn-helix motif of Fra2 and the C-terminal segment of Grx3Grx, different from the previously identified covalent heterodimer mediated by Fe-S cluster. Iron 326-330 Bol2p Saccharomyces cerevisiae S288C 101-105 22309771-3 2012 We recently demonstrated that the yeast homologues of human Glrx3 and the yeast BolA-like protein Fra2 form [2Fe-2S]-bridged heterodimers that play a key role in signaling intracellular iron availability. Iron 186-190 Bol2p Saccharomyces cerevisiae S288C 98-102 20978135-2 2011 Genetic studies indicate that Grx3/4 and Fra2 regulate activity of the iron-responsive transcription factors Aft1 and Aft2 in response to mitochondrial Fe-S cluster biosynthesis. Iron 71-75 Bol2p Saccharomyces cerevisiae S288C 41-45 20978135-2 2011 Genetic studies indicate that Grx3/4 and Fra2 regulate activity of the iron-responsive transcription factors Aft1 and Aft2 in response to mitochondrial Fe-S cluster biosynthesis. Iron 152-156 Bol2p Saccharomyces cerevisiae S288C 41-45 20978135-3 2011 We have previously shown that Fra2 and Grx3/4 form a [2Fe-2S](2+)-bridged heterodimeric complex with iron ligands provided by the active site cysteine of Grx3/4, glutathione, and a histidine residue. Iron 101-105 Bol2p Saccharomyces cerevisiae S288C 30-34 20978135-4 2011 To further characterize this unusual Fe-S-binding complex, site-directed mutagenesis was used to identify specific residues in Fra2 that influence Fe-S cluster binding and regulation of Aft1 activity in vivo. Iron 37-41 Bol2p Saccharomyces cerevisiae S288C 127-131 20978135-4 2011 To further characterize this unusual Fe-S-binding complex, site-directed mutagenesis was used to identify specific residues in Fra2 that influence Fe-S cluster binding and regulation of Aft1 activity in vivo. Iron 147-151 Bol2p Saccharomyces cerevisiae S288C 127-131 20978135-5 2011 Here, we present spectroscopic evidence that His-103 in Fra2 is an Fe-S cluster ligand in the Fra2-Grx3 complex. Iron 67-69 Bol2p Saccharomyces cerevisiae S288C 56-60 20978135-5 2011 Here, we present spectroscopic evidence that His-103 in Fra2 is an Fe-S cluster ligand in the Fra2-Grx3 complex. Iron 67-69 Bol2p Saccharomyces cerevisiae S288C 94-98 20978135-7 2011 In vivo genetic studies further confirm that Fra2 His-103 is critical for control of Aft1 activity in response to the cellular iron status. Iron 127-131 Bol2p Saccharomyces cerevisiae S288C 45-49 20978135-9 2011 Taken together, these results demonstrate that the histidine coordination and stability of the [2Fe-2S] cluster in the Fra2-Grx3 complex are essential for iron regulation in yeast. Iron 155-159 Bol2p Saccharomyces cerevisiae S288C 119-123 18281282-0 2008 Identification of FRA1 and FRA2 as genes involved in regulating the yeast iron regulon in response to decreased mitochondrial iron-sulfur cluster synthesis. Iron 74-78 Bol2p Saccharomyces cerevisiae S288C 27-31 18281282-0 2008 Identification of FRA1 and FRA2 as genes involved in regulating the yeast iron regulon in response to decreased mitochondrial iron-sulfur cluster synthesis. Iron 126-130 Bol2p Saccharomyces cerevisiae S288C 27-31 18281282-2 2008 Using a genetic screen, we identified two novel cytosolic proteins, Fra1 and Fra2, that are part of a complex that interprets the signal derived from mitochondrial Fe-S synthesis. Iron 164-168 Bol2p Saccharomyces cerevisiae S288C 77-81 18281282-3 2008 We found that mutations in FRA1 (YLL029W) and FRA2 (YGL220W) led to an increase in transcription of the iron regulon. Iron 104-108 Bol2p Saccharomyces cerevisiae S288C 46-50 18281282-7 2008 We show that Fra1 and Fra2 interact in the cytosol in an iron-independent fashion. Iron 57-61 Bol2p Saccharomyces cerevisiae S288C 22-26 18281282-8 2008 The Fra1-Fra2 complex binds to Grx3 and Grx4, two cytosolic monothiol glutaredoxins, in an iron-independent fashion. Iron 91-95 Bol2p Saccharomyces cerevisiae S288C 9-13 29038973-12 2017 The dehydratase activity was significantly improved by targeting its expression to mitochondria or by altering the Fe-S cluster metabolism of the cells with FRA2 deletion. Iron 115-119 Bol2p Saccharomyces cerevisiae S288C 157-161 24895027-7 2014 Two lines of evidence confirm that Fra2 dominates basal repression of the Aft1/2 regulons in iron-replete cultures. Iron 93-97 Bol2p Saccharomyces cerevisiae S288C 35-39 20978135-0 2011 Histidine 103 in Fra2 is an iron-sulfur cluster ligand in the [2Fe-2S] Fra2-Grx3 complex and is required for in vivo iron signaling in yeast. Iron 28-32 Bol2p Saccharomyces cerevisiae S288C 17-21 20978135-0 2011 Histidine 103 in Fra2 is an iron-sulfur cluster ligand in the [2Fe-2S] Fra2-Grx3 complex and is required for in vivo iron signaling in yeast. Iron 28-32 Bol2p Saccharomyces cerevisiae S288C 71-75 20978135-0 2011 Histidine 103 in Fra2 is an iron-sulfur cluster ligand in the [2Fe-2S] Fra2-Grx3 complex and is required for in vivo iron signaling in yeast. Iron 117-121 Bol2p Saccharomyces cerevisiae S288C 17-21 20978135-0 2011 Histidine 103 in Fra2 is an iron-sulfur cluster ligand in the [2Fe-2S] Fra2-Grx3 complex and is required for in vivo iron signaling in yeast. Iron 117-121 Bol2p Saccharomyces cerevisiae S288C 71-75 20978135-1 2011 The BolA homologue Fra2 and the cytosolic monothiol glutaredoxins Grx3 and Grx4 together play a key role in regulating iron homeostasis in Saccharomyces cerevisiae. Iron 119-123 Bol2p Saccharomyces cerevisiae S288C 19-23 19715344-8 2009 Taken together, our analytical, spectroscopic, and mutagenesis data indicate that Grx3/4 and Fra2 form a Fe-S-bridged heterodimeric complex with Fe ligands provided by the active site cysteine of Grx3/4, glutathione, and a histidine residue. Iron 105-109 Bol2p Saccharomyces cerevisiae S288C 93-97 19715344-8 2009 Taken together, our analytical, spectroscopic, and mutagenesis data indicate that Grx3/4 and Fra2 form a Fe-S-bridged heterodimeric complex with Fe ligands provided by the active site cysteine of Grx3/4, glutathione, and a histidine residue. Iron 105-107 Bol2p Saccharomyces cerevisiae S288C 93-97 19715344-9 2009 Overall, these results suggest that the ability of the Fra2-Grx3/4 complex to assemble a [2Fe-2S] cluster may act as a signal to control the iron regulon in response to cellular iron status in yeast. Iron 141-145 Bol2p Saccharomyces cerevisiae S288C 55-59 19715344-9 2009 Overall, these results suggest that the ability of the Fra2-Grx3/4 complex to assemble a [2Fe-2S] cluster may act as a signal to control the iron regulon in response to cellular iron status in yeast. Iron 178-182 Bol2p Saccharomyces cerevisiae S288C 55-59