PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 16328372-5 2006 In contrast, deletion of the AFT1 and AFT2 transcription factor genes blocked the HU activation of a subset of the Aft regulon and the aft1Delta aft2Delta double mutant was hypersensitive to HU in an iron-suppressible manner. Iron 200-204 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 29-33 17074835-9 2006 The phenotypes of the double mutant grx3grx4 characterised in this study were mainly mediated by the Aft1 function, suggesting that grx3grx4 could be a suitable cellular model for studying endogenous oxidative stress induced by deregulation of the iron homeostasis. Iron 248-252 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 101-105 16648636-0 2006 Role of glutaredoxin-3 and glutaredoxin-4 in the iron regulation of the Aft1 transcriptional activator in Saccharomyces cerevisiae. Iron 49-53 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 72-76 16648636-1 2006 The transcription factors Aft1 and Aft2 from Saccharomyces cerevisiae regulate the expression of genes involved in iron homeostasis. Iron 115-119 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 26-30 16648636-3 2006 Iron inhibition of Aft1/Aft2 was previously shown to be dependent on mitochondrial components required for cytosolic iron sulfur protein biogenesis. Iron 0-4 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 19-23 16648636-4 2006 We presently show that the nuclear monothiol glutaredoxins Grx3 and Grx4 are critical for iron inhibition of Aft1 in yeast cells. Iron 90-94 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 109-113 16648636-9 2006 The interaction between glutaredoxins and Aft1 is not modulated by the iron status of cells but is dependent on the conserved glutaredoxin domain Cys residue. Iron 71-75 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 42-46 16648636-10 2006 Thus, Grx3 and Grx4 are novel components required for Aft1 iron regulation that most likely occurs in the nucleus. Iron 59-63 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 54-58 14629196-6 2004 Siderophore uptake from different sources was also increased, and these effects were dependent on the AFT1 iron sensor regulator. Iron 107-111 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 102-106 16024809-1 2005 The yeast Saccharomyces cerevisiae contains a pair of paralogous iron-responsive transcription activators, Aft1 and Aft2. Iron 65-69 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 107-111 16024809-2 2005 Aft1 activates the cell surface iron uptake systems in iron depletion, while the role of Aft2 remains poorly understood. Iron 32-36 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 0-4 16024809-2 2005 Aft1 activates the cell surface iron uptake systems in iron depletion, while the role of Aft2 remains poorly understood. Iron 55-59 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 0-4 16024809-3 2005 This study compares the functions of Aft1 and Aft2 in regulating the transcription of genes involved in iron homeostasis, with reference to the presence/absence of the paralog. Iron 104-108 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 37-41 16024809-7 2005 Computer analysis found different cis-regulatory elements for Aft1 and Aft2, and transcription analysis using variants of the FET3 promoter indicated that Aft1 is more specific for the canonical iron-responsive element TGCACCC than is Aft2. Iron 195-199 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 155-159 16024809-8 2005 Finally, the absence of either Aft1 or Aft2 showed an iron-dependent increase in the amount of the remaining paralog. Iron 54-58 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 31-35 15725072-1 2005 In Saccharomyces cerevisiae, the transcription factor Aft1p plays a central role in regulating many genes involved in iron acquisition and utilization. Iron 118-122 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 54-59 15725072-3 2005 To identify the functional counterpart of AFT1 in Candida albicans, we transformed a C. albicans genomic DNA library into aft1Delta to isolate genes that could allow the mutant to grow under iron-limiting conditions. Iron 191-195 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 42-46 15649888-0 2005 Activation of the iron regulon by the yeast Aft1/Aft2 transcription factors depends on mitochondrial but not cytosolic iron-sulfur protein biogenesis. Iron 119-123 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 44-48 15649888-1 2005 Two transcriptional activators, Aft1 and Aft2, regulate iron homeostasis in Saccharomyces cerevisiae. Iron 56-60 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 32-36 15649888-3 2005 Iron inhibition of Aft1/Aft2 is abrogated in cells defective for Fe-S cluster biogenesis within the mitochondrial matrix (Chen, O. S., Crisp, R. J., Valachovic, M., Bard, M., Winge, D. R., and Kaplan, J. Iron 0-4 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 19-23 15649888-3 2005 Iron inhibition of Aft1/Aft2 is abrogated in cells defective for Fe-S cluster biogenesis within the mitochondrial matrix (Chen, O. S., Crisp, R. J., Valachovic, M., Bard, M., Winge, D. R., and Kaplan, J. Iron 65-69 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 19-23 15649888-7 2005 To determine whether iron sensing by Aft1/Aft2 requires the function of the mitochondrial Fe-S export and cytosolic Fe-S protein assembly systems, we evaluated the expression of the iron regulon in cells depleted of glutathione and in cells depleted of Atm1, Nar1, Cfd1, and Nbp35. Iron 21-25 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 37-41 15649888-8 2005 The iron regulon is induced in cells depleted of Atm1 with Aft1 largely responsible for the induced gene expression. Iron 4-8 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 59-63 15649888-12 2005 Thus, iron sensing by Aft1/Aft2 is not linked to the maturation of cytosolic/nuclear Fe-S proteins, but the mitochondrial inner membrane transporter Atm1 is important to transport the inhibitory signal. Iron 6-10 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 22-26 15649888-13 2005 Although Aft1 and Aft2 sense a signal emanating from the Fe-S cluster biogenesis pathway, there is no indication that the proteins are inhibited by direct binding of an Fe-S cluster. Iron 57-61 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 9-13 15133041-9 2004 Microarray experiments with cti6 mutants grown under iron-limiting conditions show a down-regulation of telomeric genes and an up-regulation of Aft1 and Tup1 target genes involved in iron and oxygen regulation. Iron 53-57 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 144-148 15133041-9 2004 Microarray experiments with cti6 mutants grown under iron-limiting conditions show a down-regulation of telomeric genes and an up-regulation of Aft1 and Tup1 target genes involved in iron and oxygen regulation. Iron 183-187 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 144-148 14993228-3 2004 The list includes several key genes in copper and iron homeostasis, such as CCC2, RCS1, FET3, LYS7, and CTR1. Iron 50-54 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 82-86 15060275-8 2004 Furthermore, YOL101c is induced by deletion of the Aft1p iron-responsive transcription factor. Iron 57-61 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 51-56 15649888-0 2005 Activation of the iron regulon by the yeast Aft1/Aft2 transcription factors depends on mitochondrial but not cytosolic iron-sulfur protein biogenesis. Iron 18-22 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 44-48 15161905-6 2004 We show that vacuolar iron transport is increased in Deltamrs3Deltamrs4 cells, resulting in decreased cytosolic iron and activation of the iron-sensing transcription factor Aft1p. Iron 22-26 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 173-178 15161905-7 2004 Activation of Aft1p leads to increased expression of the high affinity iron transport system and increased iron uptake. Iron 71-75 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 14-19 15161905-7 2004 Activation of Aft1p leads to increased expression of the high affinity iron transport system and increased iron uptake. Iron 107-111 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 14-19 15161905-11 2004 Decreased copper resistance in Deltamrs3Deltamrs4 cells results from activation of Aft1p by Ccc1p-mediated iron depletion, as deletion of CCC1 or AFT1 in Deltamrs3Deltamrs4 cells restores copper resistance. Iron 107-111 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 83-88 14722110-6 2004 Many Aft1-dependent genes involved in iron utilization that are up-regulated in a frataxin mutant were also up-regulated in the absence of Grx5. Iron 38-42 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 5-9 14722110-7 2004 BIO5 is another Aft1-dependent gene induced both upon iron deprivation and in Deltagrx5 cells; this links iron and biotin metabolism. Iron 54-58 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 16-20 14739928-2 2004 FRE2 encoding a plasma membrane ferric reductase is induced by the iron-responsive, DNA-binding, transcriptional activator Aft1. Iron 67-71 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 123-127 14739928-8 2004 Therefore, transcriptional activation in response to iron availability involves multiple protein interactions between the Aft1 iron-responsive DNA-binding factor and global regulators such as Nhp6 and Ssn6. Iron 53-57 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 122-126 14739928-8 2004 Therefore, transcriptional activation in response to iron availability involves multiple protein interactions between the Aft1 iron-responsive DNA-binding factor and global regulators such as Nhp6 and Ssn6. Iron 127-131 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 122-126 14523005-8 2003 Supporting this is that the induction of an Aft1p target gene, FTR1, in response to iron starvation was greatly reduced in pse1-1 cells. Iron 84-88 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 44-49 14668481-1 2004 The budding yeast Saccharomyces cerevisiae responds to depletion of iron in the environment by activating Aft1p, the major iron-dependent transcription factor, and by transcribing systems involved in the uptake of iron. Iron 68-72 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 106-111 14668481-1 2004 The budding yeast Saccharomyces cerevisiae responds to depletion of iron in the environment by activating Aft1p, the major iron-dependent transcription factor, and by transcribing systems involved in the uptake of iron. Iron 83-87 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 106-111 14668481-1 2004 The budding yeast Saccharomyces cerevisiae responds to depletion of iron in the environment by activating Aft1p, the major iron-dependent transcription factor, and by transcribing systems involved in the uptake of iron. Iron 83-87 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 106-111 14668481-2 2004 Here, we have studied the transcriptional response to iron deprivation and have identified new Aft1p target genes. Iron 54-58 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 95-100 14523005-0 2003 Pse1p mediates the nuclear import of the iron-responsive transcription factor Aft1p in Saccharomyces cerevisiae. Iron 41-45 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 78-83 14523005-1 2003 In Saccharomyces cerevisiae, the iron-responsive transcription factor Aft1p plays a critical role in maintaining iron homeostasis. Iron 33-37 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 70-75 14523005-1 2003 In Saccharomyces cerevisiae, the iron-responsive transcription factor Aft1p plays a critical role in maintaining iron homeostasis. Iron 113-117 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 70-75 14523005-9 2003 Furthermore, we demonstrated that the nuclear localization of a mutant Aft1 protein that contains an NLS derived from SV40 was regulated by iron status regardless of whether Pse1p could interact with Aft1p. Iron 140-144 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 71-75 14523005-2 2003 The activity of Aft1p is induced in response to iron starvation and as a consequence the expression of the iron-regulon is increased. Iron 48-52 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 16-21 14523005-2 2003 The activity of Aft1p is induced in response to iron starvation and as a consequence the expression of the iron-regulon is increased. Iron 107-111 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 16-21 14523005-3 2003 We have shown previously that Aft1p is localized to the cytoplasm under iron-replete conditions but that it is localized to the nucleus under iron-depleted conditions. Iron 72-76 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 30-35 12960168-5 2003 The transcriptional factor Aft1p, which is known to control their induction in response to iron limitation, is also required for their induction during the diauxic shift. Iron 91-95 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 27-32 14523005-3 2003 We have shown previously that Aft1p is localized to the cytoplasm under iron-replete conditions but that it is localized to the nucleus under iron-depleted conditions. Iron 142-146 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 30-35 14523005-5 2003 In pse1-1 cells, which bear a temperature-sensitive mutation of PSE1, Aft1p was misdirected to the cytoplasm during iron starvation at the restrictive temperature. Iron 116-120 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 70-75 12960168-9 2003 Thus, the two signals, glucose exhaustion and iron starvation, use two independent pathways to activate the same set of genes through the Aft1p transcriptional factor. Iron 46-50 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 138-143 12840010-2 2003 HMX1 is also transcribed under conditions of iron deprivation and is under the control of the major iron-dependent transcription factor, Aft1p. Iron 100-104 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 137-142 12928433-6 2003 We demonstrated that under heme-depleted conditions, Aft1p-GFP was able to cycle normally between the nucleus and cytosol in response to cytosolic iron. Iron 147-151 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 53-58 12928433-7 2003 Despite the inability to induce transcription under low iron conditions, chromatin immunoprecipitation demonstrated that Aft1p binds to the FET3 promoter in the absence of heme. Iron 56-60 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 121-126 12840010-7 2003 These alterations in the regulatory pools of iron lead to activation of Aft1p and inappropriate activation of heme-dependent transcription factors. Iron 45-49 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 72-77 12756250-0 2003 Aft1p and Aft2p mediate iron-responsive gene expression in yeast through related promoter elements. Iron 24-28 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 0-5 11877447-1 2002 The Aft1 transcription factor regulates the iron regulon in response to iron availability in Saccharomyces cerevisiae. Iron 44-48 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 4-8 12095998-7 2002 First, we found that FET4 expression is induced in iron-limited cells by the Aft1 iron-responsive transcriptional activator. Iron 51-55 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 77-81 12095998-7 2002 First, we found that FET4 expression is induced in iron-limited cells by the Aft1 iron-responsive transcriptional activator. Iron 82-86 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 77-81 12176980-3 2002 This response is dependent on the Aft1 protein, a transcriptional factor known to regulate a set of genes involved in iron uptake and homeostasis (iron regulon). Iron 118-122 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 34-38 12176980-3 2002 This response is dependent on the Aft1 protein, a transcriptional factor known to regulate a set of genes involved in iron uptake and homeostasis (iron regulon). Iron 147-151 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 34-38 12176980-4 2002 Like iron starvation, cobalt stress induces accumulation of the Aft1 protein in the nucleus to activate transcription of its target genes. Iron 5-9 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 64-68 12176980-6 2002 Cobalt resistance correlates with an increase in intracellular iron in AFT1-1(up) cells, and sensitivity of aft1 cells is associated with a lack of iron accumulation. Iron 63-67 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 71-75 12176980-6 2002 Cobalt resistance correlates with an increase in intracellular iron in AFT1-1(up) cells, and sensitivity of aft1 cells is associated with a lack of iron accumulation. Iron 148-152 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 108-112 12176980-7 2002 Furthermore, elevated iron levels in the growth medium suppress the cobalt sensitivity of the aft1 mutant cells, even though they increase cellular cobalt. Iron 22-26 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 94-98 12176980-8 2002 Results presented indicate that yeast cells acquire cobalt tolerance by activating the Aft1p-dependent iron regulon and thereby increasing intracellular iron levels. Iron 103-107 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 87-92 12176980-8 2002 Results presented indicate that yeast cells acquire cobalt tolerance by activating the Aft1p-dependent iron regulon and thereby increasing intracellular iron levels. Iron 153-157 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 87-92 12196168-1 2002 The budding yeast Saccharomyces cerevisiae responds to growth in limiting amounts of iron by activating the transcription factor Aft1p and expressing a set of genes that ameliorate the effects of iron deprivation. Iron 85-89 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 129-134 12196168-2 2002 Analysis of iron-regulated gene expression using cDNA microarrays has revealed the set of genes controlled by iron and Aft1p. Iron 12-16 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 119-124 11877447-0 2002 Subcellular localization of Aft1 transcription factor responds to iron status in Saccharomyces cerevisiae. Iron 66-70 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 28-32 11877447-1 2002 The Aft1 transcription factor regulates the iron regulon in response to iron availability in Saccharomyces cerevisiae. Iron 72-76 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 4-8 11877447-2 2002 Aft1 activates a battery of genes required for iron uptake under iron-starved conditions, whereas Aft1 function is inactivated under iron-replete conditions. Iron 47-51 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 0-4 11877447-2 2002 Aft1 activates a battery of genes required for iron uptake under iron-starved conditions, whereas Aft1 function is inactivated under iron-replete conditions. Iron 65-69 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 0-4 11877447-2 2002 Aft1 activates a battery of genes required for iron uptake under iron-starved conditions, whereas Aft1 function is inactivated under iron-replete conditions. Iron 65-69 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 0-4 11877447-3 2002 Previously, we have shown that iron-regulated DNA binding by Aft1 is responsible for the controlled expression of target genes. Iron 31-35 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 61-65 11877447-4 2002 Here we show that this iron-regulated DNA binding by Aft1 is not due to the change in the total expression level of Aft1 or alteration of DNA binding activity. Iron 23-27 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 53-57 11877447-5 2002 Rather, nuclear localization of Aft1 responds to iron status, leading to iron-regulated expression of the target genes. Iron 49-53 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 32-36 11877447-5 2002 Rather, nuclear localization of Aft1 responds to iron status, leading to iron-regulated expression of the target genes. Iron 73-77 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 32-36 11877447-8 2002 These results suggest that the nuclear export of Aft1 is critical for ensuring iron-responsive transcriptional activation of the Aft1 regulon and that the nuclear import/export systems are involved in iron sensing by Aft1 in S. cerevisiae. Iron 79-83 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 49-53 11877447-8 2002 These results suggest that the nuclear export of Aft1 is critical for ensuring iron-responsive transcriptional activation of the Aft1 regulon and that the nuclear import/export systems are involved in iron sensing by Aft1 in S. cerevisiae. Iron 79-83 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 129-133 11877447-8 2002 These results suggest that the nuclear export of Aft1 is critical for ensuring iron-responsive transcriptional activation of the Aft1 regulon and that the nuclear import/export systems are involved in iron sensing by Aft1 in S. cerevisiae. Iron 79-83 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 129-133 11877447-8 2002 These results suggest that the nuclear export of Aft1 is critical for ensuring iron-responsive transcriptional activation of the Aft1 regulon and that the nuclear import/export systems are involved in iron sensing by Aft1 in S. cerevisiae. Iron 201-205 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 129-133 11877447-8 2002 These results suggest that the nuclear export of Aft1 is critical for ensuring iron-responsive transcriptional activation of the Aft1 regulon and that the nuclear import/export systems are involved in iron sensing by Aft1 in S. cerevisiae. Iron 201-205 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 129-133 11829747-8 2002 Iron regulation of SMF3 was dramatically reduced, but not completely eliminated, in strains lacking both the AFT1 and AFT2 iron regulatory factors. Iron 0-4 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 109-113 12051835-12 2002 Sequences downstream of -490 harbor a consensus binding site for the iron regulatory factor Aft1p that is essential for iron regulation in wild-type strains. Iron 69-73 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 92-97 12051835-13 2002 In addition, a secondary mode of iron regulation becomes evident in strains lacking AFT1. Iron 33-37 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 84-88 11850263-6 2002 These genes exhibit similar expression patterns, and several are known to be regulated by AFT1, a DNA binding protein, which responds to iron levels in the cell. Iron 137-141 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 90-94 11734641-0 2001 A second iron-regulatory system in yeast independent of Aft1p. Iron 9-13 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 56-61 11673473-1 2001 Analysis of iron-regulated gene expression in Saccharomyces cerevisiae using cDNA microarrays has identified three putative cell wall proteins that are directly regulated by Aft1p, the major iron-dependent transcription factor in yeast. Iron 12-16 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 174-179 11673473-2 2001 FIT1, FIT2, and FIT3 (for facilitator of iron transport) were more highly expressed in strains grown in low concentrations of iron and in strains in which AFT1-1(up), a constitutively active allele of AFT1, was expressed. Iron 41-45 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 155-159 11673473-2 2001 FIT1, FIT2, and FIT3 (for facilitator of iron transport) were more highly expressed in strains grown in low concentrations of iron and in strains in which AFT1-1(up), a constitutively active allele of AFT1, was expressed. Iron 41-45 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 201-205 11673473-2 2001 FIT1, FIT2, and FIT3 (for facilitator of iron transport) were more highly expressed in strains grown in low concentrations of iron and in strains in which AFT1-1(up), a constitutively active allele of AFT1, was expressed. Iron 126-130 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 155-159 11673473-2 2001 FIT1, FIT2, and FIT3 (for facilitator of iron transport) were more highly expressed in strains grown in low concentrations of iron and in strains in which AFT1-1(up), a constitutively active allele of AFT1, was expressed. Iron 126-130 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 201-205 11673473-3 2001 Northern blot analysis confirmed that FIT1, FIT2, and FIT3 mRNA transcript levels were increased 60-230-fold in response to iron deprivation in an Aft1p-dependent manner. Iron 124-128 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 147-152 11673473-6 2001 FIT-deletion strains exhibited increased expression of Aft1p target genes as measured by a FET3-lacZ reporter gene or by Arn1p Western blotting, indicating that cells respond to the absence of FIT genes by up-regulating systems of iron uptake. Iron 231-235 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 55-60 11673473-7 2001 Aft1p activation in FIT-deleted strains occurred when either ferrichrome or ferric salts were used as sources of iron during growth, suggesting that the FIT genes enhance uptake of iron from both sources. Iron 113-117 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 0-5 11673473-7 2001 Aft1p activation in FIT-deleted strains occurred when either ferrichrome or ferric salts were used as sources of iron during growth, suggesting that the FIT genes enhance uptake of iron from both sources. Iron 181-185 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 0-5 11734641-1 2001 Iron homeostasis in the yeast Saccharomyces cerevisiae is regulated at the transcriptional level by Aft1p, which activates the expression of its target genes in response to low-iron conditions. Iron 0-4 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 100-105 11734641-1 2001 Iron homeostasis in the yeast Saccharomyces cerevisiae is regulated at the transcriptional level by Aft1p, which activates the expression of its target genes in response to low-iron conditions. Iron 177-181 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 100-105 11734641-13 2001 Together, these data suggest that yeast has a second regulatory pathway for the iron regulon, with AFT1 and AFT2 playing partially redundant roles. Iron 80-84 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 99-103 11448968-0 2001 Aft2p, a novel iron-regulated transcription activator that modulates, with Aft1p, intracellular iron use and resistance to oxidative stress in yeast. Iron 15-19 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 75-80 11448968-10 2001 These results suggest that Aft2p and Aft1p have overlapping roles in the control of iron-regulated pathway(s) connected to oxidative stress resistance in yeast. Iron 84-88 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 37-42 11448968-0 2001 Aft2p, a novel iron-regulated transcription activator that modulates, with Aft1p, intracellular iron use and resistance to oxidative stress in yeast. Iron 96-100 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 75-80 11448968-1 2001 The yeast, Saccharomyces cerevisiae, contains a transcription activator, Aft1p, that regulates the transcription of the high affinity iron transport system genes. Iron 134-138 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 73-78 11448968-5 2001 The double aft1aft2 mutant was unable to grow in iron-deprived conditions. Iron 49-53 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 11-19 11120744-4 2001 The yeast genome contains five additional FRE1 and FRE2 homologues, four of which are regulated by iron and the major iron-dependent transcription factor, Aft1p, but whose function remains unknown. Iron 118-122 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 155-160 11112771-4 2001 Frataxin deficiency results in enhanced expression of some 70 genes including a set of genes, called the iron regulon, that are under the control of the iron-sensing transcription factor AFT1. Iron 105-109 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 187-191 11112771-4 2001 Frataxin deficiency results in enhanced expression of some 70 genes including a set of genes, called the iron regulon, that are under the control of the iron-sensing transcription factor AFT1. Iron 153-157 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 187-191 11112771-11 2001 Our data show that frataxin deficiency elicits iron mobilization from all iron sources in an AFT1-dependent manner. Iron 47-51 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 93-97 11112771-11 2001 Our data show that frataxin deficiency elicits iron mobilization from all iron sources in an AFT1-dependent manner. Iron 74-78 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 93-97 10713071-6 2000 Introduction of AFT1(up), a constitutive allele of the iron transcription factor, AFT1, that also leads to increased high affinity iron transport did not prevent Deltayfh1 cells from becoming respiratory-incompetent. Iron 55-59 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 16-20 10748025-2 2000 A family of four putative transporters (Arn1p-4p) in Saccharomyces cerevisiae is expressed under conditions of iron deprivation and is regulated by Aft1p, the major iron-dependent transcription factor in yeast. Iron 111-115 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 148-153 10748025-2 2000 A family of four putative transporters (Arn1p-4p) in Saccharomyces cerevisiae is expressed under conditions of iron deprivation and is regulated by Aft1p, the major iron-dependent transcription factor in yeast. Iron 165-169 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 148-153 10744769-2 2000 In the yeast Saccharomyces cerevisiae, uptake of iron is largely regulated by the transcription factor Aft1. Iron 49-53 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 103-107 11223939-3 2001 We have developed an in-plate protocol for the isolation of clones that complement an aft1 mutation in S. cerevisiae that makes cells dependent on iron for growth. Iron 147-151 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 86-90 10713071-6 2000 Introduction of AFT1(up), a constitutive allele of the iron transcription factor, AFT1, that also leads to increased high affinity iron transport did not prevent Deltayfh1 cells from becoming respiratory-incompetent. Iron 55-59 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 82-86 10713071-6 2000 Introduction of AFT1(up), a constitutive allele of the iron transcription factor, AFT1, that also leads to increased high affinity iron transport did not prevent Deltayfh1 cells from becoming respiratory-incompetent. Iron 131-135 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 16-20 10713071-6 2000 Introduction of AFT1(up), a constitutive allele of the iron transcription factor, AFT1, that also leads to increased high affinity iron transport did not prevent Deltayfh1 cells from becoming respiratory-incompetent. Iron 131-135 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 82-86 9724638-0 1998 Cell-cycle arrest and inhibition of G1 cyclin translation by iron in AFT1-1(up) yeast. Iron 61-65 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 69-73 10551871-4 1999 This mutant exhibited constitutive up-regulation of the genes of the cellular iron uptake system, mediated through effects on the Aft1p iron-regulatory protein. Iron 78-82 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 130-135 10588895-4 1999 Both genes are induced in strains expressing an activated allele of Aft1p, the iron-sensing transcription factor, suggesting that they are regulated by the iron status of the cell. Iron 79-83 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 68-73 10588895-4 1999 Both genes are induced in strains expressing an activated allele of Aft1p, the iron-sensing transcription factor, suggesting that they are regulated by the iron status of the cell. Iron 156-160 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 68-73 10341420-3 1999 They both encode for plasma membrane ferric/cupric reductases and their expression is regulated by iron and copper availability, mediated by the transcription factors Aft1p and Mac1p, respectively. Iron 99-103 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 167-172 9724638-2 1998 In yeast, iron uptake is homeostatically regulated by the transcription factor encoded by AFT1. Iron 10-14 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 90-94 9724638-3 1998 Expression of AFT1-1(up), a dominant mutant allele, results in inappropriately high rates of iron uptake, and AFT1-1(up) mutants grow slowly in the presence of high concentrations of iron. Iron 93-97 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 14-18 9724638-3 1998 Expression of AFT1-1(up), a dominant mutant allele, results in inappropriately high rates of iron uptake, and AFT1-1(up) mutants grow slowly in the presence of high concentrations of iron. Iron 183-187 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 14-18 9724638-3 1998 Expression of AFT1-1(up), a dominant mutant allele, results in inappropriately high rates of iron uptake, and AFT1-1(up) mutants grow slowly in the presence of high concentrations of iron. Iron 183-187 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 110-114 9724638-4 1998 We present evidence that when Aft1-1(up) mutants are exposed to iron, they arrest the cell division cycle at the G1 regulatory point Start. Iron 64-68 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 30-34 9200812-0 1997 The AFT1 transcriptional factor is differentially required for expression of high-affinity iron uptake genes in Saccharomyces cerevisiae. Iron 91-95 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 4-8 9726978-4 1998 Four of the homologs (FRE3-FRE6) are specifically iron-regulated through the Aft1 transcription factor. Iron 50-54 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 77-81 9726978-6 1998 Expression of FRE3-FRE6 is elevated in AFT1-1 cells and attenuated in aft1 null cells, showing that iron modulation occurs through the Aft1 transcriptional activator. Iron 100-104 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 39-43 9726978-6 1998 Expression of FRE3-FRE6 is elevated in AFT1-1 cells and attenuated in aft1 null cells, showing that iron modulation occurs through the Aft1 transcriptional activator. Iron 100-104 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 70-74 9726978-6 1998 Expression of FRE3-FRE6 is elevated in AFT1-1 cells and attenuated in aft1 null cells, showing that iron modulation occurs through the Aft1 transcriptional activator. Iron 100-104 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 135-139 9516467-7 1998 The anaerobic repression was reduced in cells expressing an iron-independent form of the trans-activator, Aft1, a protein that regulates the expression of these proteins. Iron 60-64 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 106-110 9516467-12 1998 The data suggest a model in which dioxygen directly or indirectly modulates the Fe(III)/Fe(II) ratio in an iron pool linked to Aft1 protein while bipyridyl increases this ratio by chelating Fe(II). Iron 107-111 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 127-131 9434348-6 1997 The inability of these same vacuolar mutant strains to repress FET3 mRNA levels in the presence of an iron-unresponsive allele of the AFT1 regulatory gene are consistent with alterations in the intracellular distribution of redox states of Fe3+/2+ in the presence of elevated extracellular concentrations of copper ions. Iron 102-106 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 134-138 9200812-5 1997 Aft1 protein is required for maintaining detectable non-induced level of FET3 expression and for induction of FRE2 in iron starvation conditions. Iron 118-122 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 0-4 9200812-8 1997 The alterations in the basal levels of expression of the high-affinity iron uptake genes may explain why the AFT1 mutant is unable to grow on respirable carbon sources. Iron 71-75 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 109-113 9200812-10 1997 Aft1 is a transcriptional activator that would be part of the different transcriptional complexes interacting with the promoter of the high-affinity iron uptake genes. Iron 149-153 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 0-4 9200812-11 1997 Aft1 displays phosphorylation modifications depending on the growth stage of the cells, and it might link induction of genes for iron uptake to other metabolically dominant requirement for cell growth. Iron 129-133 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 0-4 7720713-8 1995 Conversely, a strain with interruption of the AFT1 gene manifests low ferric reductase and ferrous iron uptake and is susceptible to iron deprivation, because of deficient expression of FRE1 and negligible expression of FRE2 and FET3. Iron 99-103 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 46-50 9083054-7 1997 As additional evidence for the role of Atx1p in iron metabolism, we find that the gene is induced by the same iron-sensing trans-activator, Aft1p, that regulates CCC2 and FET3. Iron 48-52 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 140-145 9083054-7 1997 As additional evidence for the role of Atx1p in iron metabolism, we find that the gene is induced by the same iron-sensing trans-activator, Aft1p, that regulates CCC2 and FET3. Iron 110-114 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 140-145 8670839-0 1996 Iron-regulated DNA binding by the AFT1 protein controls the iron regulon in yeast. Iron 0-4 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 34-38 8670839-0 1996 Iron-regulated DNA binding by the AFT1 protein controls the iron regulon in yeast. Iron 60-64 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 34-38 8670839-6 1996 In vivo footprinting demonstrated occupancy of the AFT1 binding site in cells deprived of iron and not in cells grown in the presence of iron. Iron 90-94 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 51-55 8670839-7 1996 Thus, the environmental signal resulting from iron deprivation was transduced through the regulated binding of AFT1 to the FET3 promoter, followed by the activation of transcription. Iron 13-17 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 111-115 7720713-0 1995 AFT1: a mediator of iron regulated transcriptional control in Saccharomyces cerevisiae. Iron 20-24 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 0-4 7720713-1 1995 Using a scheme for selecting mutants of Saccharomyces cerevisiae with abnormalities of iron metabolism, we have identified a gene, AFT1, that mediates the control of iron uptake. Iron 87-91 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 131-135 7720713-1 1995 Using a scheme for selecting mutants of Saccharomyces cerevisiae with abnormalities of iron metabolism, we have identified a gene, AFT1, that mediates the control of iron uptake. Iron 166-170 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 131-135 7720713-4 1995 A dominant mutant allele of this gene, termed AFT1-1up, results in high levels of ferric reductase and ferrous iron uptake that are not repressed by exogenous iron. Iron 111-115 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 46-50 7720713-4 1995 A dominant mutant allele of this gene, termed AFT1-1up, results in high levels of ferric reductase and ferrous iron uptake that are not repressed by exogenous iron. Iron 159-163 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 46-50 7720713-8 1995 Conversely, a strain with interruption of the AFT1 gene manifests low ferric reductase and ferrous iron uptake and is susceptible to iron deprivation, because of deficient expression of FRE1 and negligible expression of FRE2 and FET3. Iron 133-137 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 46-50 7720713-9 1995 Thus, AFT1 functions to activate transcription of target genes in response to iron deprivation and thereby plays a central role in iron homeostasis. Iron 78-82 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 6-10 7720713-9 1995 Thus, AFT1 functions to activate transcription of target genes in response to iron deprivation and thereby plays a central role in iron homeostasis. Iron 131-135 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 6-10 34029365-4 2021 show that like central carbon metabolism, iron metabolism is also closely implicated in autophagy-mediated life extension via the TORC2 activator Ypk1p and the iron regulator Aft1p. Iron 42-46 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 175-180 34829190-7 2021 The transcriptional activation function of the iron-sensing Aft1 factor is interrupted due to its mislocalization to the vacuole. Iron 47-51 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 60-64 26323600-6 2015 Furthermore, the strain transformed with AFT1-1(up) plasmid, which failed to regulate Aft1 via iron concentration and accumulated Aft1 in the nucleus, showed lower FOB uptake activity. Iron 95-99 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 41-45 35218933-3 2022 Thus, in response to iron deficiency, transcription factors Aft1 and Aft2 activate the expression of genes implicated in iron acquisition and mobilization, whereas two mRNA-binding proteins, Cth1 and Cth2, posttranscriptionally control iron metabolism. Iron 121-125 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 60-64 35218933-3 2022 Thus, in response to iron deficiency, transcription factors Aft1 and Aft2 activate the expression of genes implicated in iron acquisition and mobilization, whereas two mRNA-binding proteins, Cth1 and Cth2, posttranscriptionally control iron metabolism. Iron 236-240 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 60-64 34029365-4 2021 show that like central carbon metabolism, iron metabolism is also closely implicated in autophagy-mediated life extension via the TORC2 activator Ypk1p and the iron regulator Aft1p. Iron 160-164 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 175-180 32147528-0 2020 The yeast Aft1 transcription factor activates ribonucleotide reductase catalytic subunit RNR1 in response to iron deficiency. Iron 109-113 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 10-14 33507238-5 2021 Iron replenishment in its turn, reduces autophagy flux through the AMPK Snf1 and the subsequent activity of the iron responsive transcription factor, Aft1. Iron 0-4 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 150-154 33507238-5 2021 Iron replenishment in its turn, reduces autophagy flux through the AMPK Snf1 and the subsequent activity of the iron responsive transcription factor, Aft1. Iron 112-116 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 150-154 33013818-4 2020 Upon iron deficiency, yeast utilizes two transcription factors, Aft1 and Aft2, to activate the expression of a set of genes known as the iron regulon, which are implicated in iron uptake, recycling and mobilization. Iron 5-9 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 64-68 33013818-4 2020 Upon iron deficiency, yeast utilizes two transcription factors, Aft1 and Aft2, to activate the expression of a set of genes known as the iron regulon, which are implicated in iron uptake, recycling and mobilization. Iron 137-141 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 64-68 33013818-5 2020 Moreover, Aft1 and Aft2 activate the expression of Cth2, an mRNA-binding protein that limits the expression of genes encoding for iron-containing proteins or that participate in iron-using processes. Iron 130-134 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 10-14 33013818-5 2020 Moreover, Aft1 and Aft2 activate the expression of Cth2, an mRNA-binding protein that limits the expression of genes encoding for iron-containing proteins or that participate in iron-using processes. Iron 178-182 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 10-14 32251724-2 2020 In response to iron deficiency, the Saccharomyces cerevisiae iron-responsive transcription factor Aft1 accumulates in the nucleus and activates a set of genes that promote iron acquisition at the cell surface. Iron 15-19 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 98-102 32251724-2 2020 In response to iron deficiency, the Saccharomyces cerevisiae iron-responsive transcription factor Aft1 accumulates in the nucleus and activates a set of genes that promote iron acquisition at the cell surface. Iron 61-65 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 98-102 32251724-2 2020 In response to iron deficiency, the Saccharomyces cerevisiae iron-responsive transcription factor Aft1 accumulates in the nucleus and activates a set of genes that promote iron acquisition at the cell surface. Iron 61-65 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 98-102 33549702-0 2021 The MAPK Slt2/Mpk1 plays a role in iron homeostasis through direct regulation of the transcription factor Aft1. Iron 35-39 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 106-110 33549702-3 2021 In budding yeast, the high affinity iron regulon is under the control of the transcription factor Aft1. Iron 36-40 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 98-102 33549702-4 2021 We present evidence demonstrating that the MAPK Slt2 of the cell wall integrity pathway (CWI), phosphorylates and negatively regulates Aft1 activity upon the iron depletion signal, both in fermentative or respiratory conditions. Iron 158-162 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 135-139 32251724-6 2020 Subcellular localization studies reveal that low UFAs cause a mislocalization of Aft1 protein to the vacuole upon iron deprivation that prevents its nuclear accumulation. Iron 114-118 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 81-85 32251724-7 2020 These results indicate that Mga2 and Ole1 are essential to maintain the UFA levels required for Aft1-dependent activation of the iron regulon in response to iron deficiency, and directly connect the biosynthesis of fatty acids to the response to iron depletion. Iron 129-133 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 96-100 32251724-7 2020 These results indicate that Mga2 and Ole1 are essential to maintain the UFA levels required for Aft1-dependent activation of the iron regulon in response to iron deficiency, and directly connect the biosynthesis of fatty acids to the response to iron depletion. Iron 157-161 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 96-100 32251724-7 2020 These results indicate that Mga2 and Ole1 are essential to maintain the UFA levels required for Aft1-dependent activation of the iron regulon in response to iron deficiency, and directly connect the biosynthesis of fatty acids to the response to iron depletion. Iron 157-161 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 96-100 32147528-3 2020 Upon iron starvation, the Saccharomyces cerevisiae Aft1 transcription factor specifically binds to iron-responsive cis elements within the promoter of a group of genes, known as the iron regulon, activating their transcription. Iron 5-9 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 51-55 32147528-3 2020 Upon iron starvation, the Saccharomyces cerevisiae Aft1 transcription factor specifically binds to iron-responsive cis elements within the promoter of a group of genes, known as the iron regulon, activating their transcription. Iron 99-103 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 51-55 32147528-3 2020 Upon iron starvation, the Saccharomyces cerevisiae Aft1 transcription factor specifically binds to iron-responsive cis elements within the promoter of a group of genes, known as the iron regulon, activating their transcription. Iron 99-103 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 51-55 32147528-6 2020 We demonstrate that Aft1 and the DNA-binding protein Ixr1 enhance the expression of the gene encoding for its catalytic subunit, RNR1, in response to iron limitation, leading to an increase in both mRNA and protein levels. Iron 150-154 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 20-24 32147528-7 2020 By mutagenesis of the Aft1-binding sites within RNR1 promoter, we conclude that RNR1 activation by iron depletion is important for Rnr1 protein and deoxyribonucleotide synthesis. Iron 99-103 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 22-26 32147528-8 2020 Remarkably, Aft1 also activates the expression of IXR1 upon iron scarcity through an iron-responsive element located within its promoter. Iron 60-64 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 12-16 32147528-8 2020 Remarkably, Aft1 also activates the expression of IXR1 upon iron scarcity through an iron-responsive element located within its promoter. Iron 85-89 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 12-16 32147528-9 2020 These results provide a novel mechanism for the direct activation of ribonucleotide reductase function by the iron-regulated Aft1 transcription factor. Iron 110-114 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 125-129 31896574-7 2020 We defined Aft1- and Yap1-dependent transcriptional sites in the MMT1 promoter that are necessary for low iron- or oxidant-mediated MMT1 expression. Iron 106-110 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 11-15 31975050-4 2020 Yeast vma mutants exhibited nuclear localization of Aft1, which turns on the iron regulon in response to iron-sulfur cluster (ISC) deficiency. Iron 77-81 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 52-56 31896574-0 2020 The mitochondrial iron exporter genes MMT1 and MMT2 in yeast are transcriptionally regulated by Aft1 and Yap1. Iron 18-22 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 96-100 31896574-3 2020 Here, we report that MMT1 and MMT2 expression is transcriptionally regulated by two pathways: the low-iron sensing transcription factor Aft1 and the oxidant-sensing transcription factor Yap1. Iron 102-106 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 136-140 31896574-9 2020 Our findings reveal that both MMT1 and MMT2 are induced under low-iron conditions and that the low-iron regulator Aft1 is required for this induction. Iron 66-70 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 114-118 31896574-9 2020 Our findings reveal that both MMT1 and MMT2 are induced under low-iron conditions and that the low-iron regulator Aft1 is required for this induction. Iron 99-103 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 114-118 31896574-10 2020 We further uncover an Aft1-binding site in the MMT1 promoter sufficient for inducing MMT1 transcription and identify an MMT2 promoter region required for low-iron induction. Iron 158-162 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 22-26 29995317-3 2018 In the yeast Saccharomyces cerevisiae, iron homeostasis is maintained by the transcriptional control of the iron acquisition systems (iron regulon), mainly by the iron-responsive transcriptional factors Aft1p and Yap5p. Iron 39-43 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 203-208 31493153-1 2019 The Saccharomyces cerevisiae transcriptional activator Aft1 and its paralog Aft2 respond to iron deficiency by upregulating expression of proteins required for iron uptake at the plasma membrane, vacuolar iron transport, and mitochondrial iron metabolism, with the net result of mobilizing iron from extracellular sources and intracellular stores. Iron 92-96 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 55-59 31493153-1 2019 The Saccharomyces cerevisiae transcriptional activator Aft1 and its paralog Aft2 respond to iron deficiency by upregulating expression of proteins required for iron uptake at the plasma membrane, vacuolar iron transport, and mitochondrial iron metabolism, with the net result of mobilizing iron from extracellular sources and intracellular stores. Iron 160-164 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 55-59 31493153-1 2019 The Saccharomyces cerevisiae transcriptional activator Aft1 and its paralog Aft2 respond to iron deficiency by upregulating expression of proteins required for iron uptake at the plasma membrane, vacuolar iron transport, and mitochondrial iron metabolism, with the net result of mobilizing iron from extracellular sources and intracellular stores. Iron 160-164 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 55-59 31493153-1 2019 The Saccharomyces cerevisiae transcriptional activator Aft1 and its paralog Aft2 respond to iron deficiency by upregulating expression of proteins required for iron uptake at the plasma membrane, vacuolar iron transport, and mitochondrial iron metabolism, with the net result of mobilizing iron from extracellular sources and intracellular stores. Iron 160-164 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 55-59 31493153-2 2019 Conversely, when iron levels are sufficient, Aft1 and Aft2 interact with the cytosolic glutaredoxins Grx3 and Grx4 and the BolA protein Bol2, which promote Aft1/2 dissociation from DNA and subsequent export from the nucleus. Iron 17-21 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 45-49 31493153-2 2019 Conversely, when iron levels are sufficient, Aft1 and Aft2 interact with the cytosolic glutaredoxins Grx3 and Grx4 and the BolA protein Bol2, which promote Aft1/2 dissociation from DNA and subsequent export from the nucleus. Iron 17-21 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 156-162 31493153-3 2019 Previous studies unveiled the molecular mechanism for iron-dependent inhibition of Aft1/2 activity, demonstrating that the [2Fe-2S]-bridged Grx3-Bol2 heterodimer transfers a cluster to Aft2, driving Aft2 dimerization and dissociation from DNA. Iron 54-58 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 83-89 30137082-6 2018 In response to iron deficiency, the yeast Aft1 transcription factor activates cellular iron acquisition. Iron 15-19 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 42-46 30137082-7 2018 However, when constitutively active, Aft1 inhibits growth probably due to iron toxicity. Iron 74-78 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 37-41 30137082-8 2018 In this report, we have studied the consequences of using hyperactive AFT1 alleles, including AFT1-1UP, to increase yeast iron accumulation. Iron 122-126 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 70-74 30137082-8 2018 In this report, we have studied the consequences of using hyperactive AFT1 alleles, including AFT1-1UP, to increase yeast iron accumulation. Iron 122-126 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 94-98 30137082-9 2018 We first characterized the iron sensitivity of cells expressing different constitutively active AFT1 alleles. Iron 27-31 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 96-100 30137082-10 2018 We rescued the high iron sensitivity conferred by the AFT1 alleles by deleting the sphingolipid signaling kinase YPK1. Iron 20-24 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 54-58 30137082-11 2018 We observed that the deletion of YPK1 exerts different effects on iron accumulation depending on the AFT1 allele and the environmental iron. Iron 66-70 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 101-105 30137082-12 2018 Moreover, we determined that the impairment of the high-affinity iron transport system partially rescues the high iron toxicity of AFT1-1UP-expressing cells. Iron 65-69 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 131-135 30137082-12 2018 Moreover, we determined that the impairment of the high-affinity iron transport system partially rescues the high iron toxicity of AFT1-1UP-expressing cells. Iron 114-118 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 131-135 29995317-3 2018 In the yeast Saccharomyces cerevisiae, iron homeostasis is maintained by the transcriptional control of the iron acquisition systems (iron regulon), mainly by the iron-responsive transcriptional factors Aft1p and Yap5p. Iron 108-112 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 203-208 29995317-3 2018 In the yeast Saccharomyces cerevisiae, iron homeostasis is maintained by the transcriptional control of the iron acquisition systems (iron regulon), mainly by the iron-responsive transcriptional factors Aft1p and Yap5p. Iron 108-112 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 203-208 29995317-3 2018 In the yeast Saccharomyces cerevisiae, iron homeostasis is maintained by the transcriptional control of the iron acquisition systems (iron regulon), mainly by the iron-responsive transcriptional factors Aft1p and Yap5p. Iron 108-112 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 203-208 29995317-8 2018 Hog1p, a MAP kinase involved in stress responses, also negatively regulates iron uptake by phosphorylating Aft1p. Iron 76-80 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 107-112 29867869-8 2018 The latter in combination with a flor-yeast-specific mutation in the Aft1 transcription factor gene is likely to be responsible for the discovered phenotype of increased iron sensitivity and improved iron uptake of analyzed strains. Iron 170-174 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 69-73 29867869-8 2018 The latter in combination with a flor-yeast-specific mutation in the Aft1 transcription factor gene is likely to be responsible for the discovered phenotype of increased iron sensitivity and improved iron uptake of analyzed strains. Iron 200-204 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 69-73 29438506-1 2018 Iron deprivation induces transcription of genes required for iron uptake, and transcription factor Aft1 and Aft2 mediate this by regulating transcriptional program in Saccharomyces cerevisiae. Iron 0-4 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 99-103 28918000-2 2018 Yfh1 deficiency activates Aft1, a transcription factor governing iron homeostasis in yeast cells. Iron 65-69 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 26-30 28918000-8 2018 This phenomenon is not observed when Aft1 is activated by iron scarcity or impaired iron-sulfur biogenesis. Iron 58-62 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 37-41 29604179-1 2018 In the yeast Saccharomyces cerevisiae Aft1, the low iron-sensing transcription factor is known to regulate the expression of the FET3 gene. Iron 52-56 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 38-42 29604179-5 2018 We suggest that the activation of FET3 by Ace1 prevents the hyper activation of Aft1, possibly by assuring the adequate functioning of mitochondrial iron-sulfur cluster biogenesis. Iron 149-153 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 80-84 29604179-6 2018 While reinforcing the link between iron and copper homeostasis, this work unveils a novel protection mechanism against copper toxicity mediated by Ace1, which relies in the activation of FET3 and results in the restriction of Aft1 activity as a means to prevent excessive copper accumulation. Iron 35-39 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 226-230 29626156-7 2018 A mutant with constitutive activity of Gcn2 (GCN2c ) shows less repression of iron transport genes by amino acids and increased nuclear localization of Aft1 in an iron-poor medium, and increases iron content in this medium. Iron 163-167 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 152-156 29626156-7 2018 A mutant with constitutive activity of Gcn2 (GCN2c ) shows less repression of iron transport genes by amino acids and increased nuclear localization of Aft1 in an iron-poor medium, and increases iron content in this medium. Iron 163-167 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 152-156 29032057-10 2018 To our knowledge, Hog1p is the first kinase reported to directly regulate Aft1p, impacting on iron homeostasis. Iron 94-98 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 74-79 29032057-0 2018 The Hog1p kinase regulates Aft1p transcription factor to control iron accumulation. Iron 65-69 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 27-32 27376881-1 2017 In the Saccharomyces cerevisiae eukaryotic model, the induction of the iron regulon genes ARN1, FIT2 and CTH2 by growth-inhibitory concentrations of alachlor (ALA) was dependent on Aft1p expression. Iron 71-75 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 181-186 29032057-2 2018 In budding yeast, the low-iron sensing transcription factor Aft1p is a master regulator of the iron regulon. Iron 26-30 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 60-65 29032057-2 2018 In budding yeast, the low-iron sensing transcription factor Aft1p is a master regulator of the iron regulon. Iron 95-99 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 60-65 29032057-6 2018 Consistently, the expression of a phosphomimetic version of Aft1p-S210/S224 that favours its nuclear export abolished iron accumulation in isc1Delta cells. Iron 118-122 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 60-65 29032057-8 2018 However, Hog1p-Aft1p interaction decreases in isc1Delta cells, which likely contributes to Aft1p dephosphorylation and consequently to Aft1p activation and iron overload in isc1Delta cells. Iron 156-160 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 15-20 28315258-4 2017 In the yeast Saccharomyces cerevisiae, transcription factors Aft1/Aft2 and Yap5 regulate iron metabolism in response to low and high iron levels, respectively. Iron 89-93 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 61-65 28315258-4 2017 In the yeast Saccharomyces cerevisiae, transcription factors Aft1/Aft2 and Yap5 regulate iron metabolism in response to low and high iron levels, respectively. Iron 133-137 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 61-65 28315258-7 2017 Various conserved proteins, such as ABC mitochondrial transporter Atm1 and, for Aft1/Aft2, monothiol glutaredoxins Grx3 and Grx4 are implicated in this iron-signaling pathway. Iron 152-156 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 80-84 28702011-5 2017 HIP HOP profiling in Saccharomyces cerevisiae using a panel of signature strains that are characteristic for common modes of action identified hypersensitivity in yeast lacking the iron-dependent transcription factor Aft1 suggesting restricted iron uptake as a mode of action. Iron 181-185 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 217-221 28702011-5 2017 HIP HOP profiling in Saccharomyces cerevisiae using a panel of signature strains that are characteristic for common modes of action identified hypersensitivity in yeast lacking the iron-dependent transcription factor Aft1 suggesting restricted iron uptake as a mode of action. Iron 244-248 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 217-221 27376881-3 2017 The hypersensitivity of the aft1Delta mutant to ALA was abrogated by surplus exogenous iron, suggesting that the role of Aft1p in ALA tolerance may be associated with iron limitation under ALA stress. Iron 87-91 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 121-126 27376881-3 2017 The hypersensitivity of the aft1Delta mutant to ALA was abrogated by surplus exogenous iron, suggesting that the role of Aft1p in ALA tolerance may be associated with iron limitation under ALA stress. Iron 167-171 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 121-126 27795405-5 2016 Specifically, similarly to baker"s yeast, Aft1 is the main positive regulator under iron starvation conditions, while Cth2 degrades mRNAs encoding iron-requiring enzymes. Iron 84-88 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 42-46 28079179-9 2017 The clustering of co-expressed genes and network analysis revealed that many iron and zinc homeostasis genes are targets of transcription factors Aft1p and Zap1p. Iron 77-81 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 146-151 26450372-1 2015 We have identified a new downstream target gene of the Aft1/2-regulated iron regulon in budding yeast Saccharomyces cerevisiae, the late-annotated small open reading frame LSO1. Iron 72-76 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 55-61 27427385-4 2016 Instead, we show that 2HG production perturbs the iron sensing mechanisms as indicated by upregulation of the Aft1-controlled iron regulon and a concomitant increase in iron levels. Iron 50-54 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 110-114 27427385-4 2016 Instead, we show that 2HG production perturbs the iron sensing mechanisms as indicated by upregulation of the Aft1-controlled iron regulon and a concomitant increase in iron levels. Iron 126-130 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 110-114 27427385-4 2016 Instead, we show that 2HG production perturbs the iron sensing mechanisms as indicated by upregulation of the Aft1-controlled iron regulon and a concomitant increase in iron levels. Iron 126-130 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 110-114 27427385-5 2016 Accordingly, iron chelation, or overexpression of a truncated AFT1 allele that dampens transcription of the iron regulon, suppresses the loss of respirative capacity. Iron 108-112 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 62-66 27618952-2 2016 We show that yeast cells lacking Aft2, a transcription factor that together with Aft1 regulates iron homeostasis, are highly sensitive to selenite but, in contrast to aft1 mutants, this is not rescued by iron supplementation. Iron 96-100 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 81-85 26450372-4 2015 In contrast, we find that the LSO1 promoter region contains three consensus binding sites for the Aft1/2 transcription factors and that an LSO1-lacZ reporter is highly induced under low-iron conditions in a Aft1-dependent manner. Iron 186-190 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 98-104 24895027-5 2014 Second, iron regulons controlled by Aft1/2, Cth2 and Yap5 that respond to mitochondrial iron-sulphur cluster status are modulated by copper-BPQ causing iron hyper-accumulation via upregulated iron-import. Iron 8-12 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 36-42 26396185-6 2015 To address whether Erv1 or Mia40 plays a role in iron regulation, we measured iron-dependent expression of Aft1/2-regulated genes and mitochondrial iron accumulation in erv1 and mia40 strains. Iron 78-82 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 107-113 26396185-6 2015 To address whether Erv1 or Mia40 plays a role in iron regulation, we measured iron-dependent expression of Aft1/2-regulated genes and mitochondrial iron accumulation in erv1 and mia40 strains. Iron 78-82 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 107-113 25300486-4 2014 Aft1 regulates iron homeostasis and is also involved in genome integrity having additional iron-independent functions. Iron 15-19 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 0-4 25300486-4 2014 Aft1 regulates iron homeostasis and is also involved in genome integrity having additional iron-independent functions. Iron 91-95 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 0-4 24895027-5 2014 Second, iron regulons controlled by Aft1/2, Cth2 and Yap5 that respond to mitochondrial iron-sulphur cluster status are modulated by copper-BPQ causing iron hyper-accumulation via upregulated iron-import. Iron 88-92 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 36-42 24895027-5 2014 Second, iron regulons controlled by Aft1/2, Cth2 and Yap5 that respond to mitochondrial iron-sulphur cluster status are modulated by copper-BPQ causing iron hyper-accumulation via upregulated iron-import. Iron 88-92 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 36-42 24895027-5 2014 Second, iron regulons controlled by Aft1/2, Cth2 and Yap5 that respond to mitochondrial iron-sulphur cluster status are modulated by copper-BPQ causing iron hyper-accumulation via upregulated iron-import. Iron 88-92 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 36-42 24895027-7 2014 Two lines of evidence confirm that Fra2 dominates basal repression of the Aft1/2 regulons in iron-replete cultures. Iron 93-97 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 74-80 24172132-1 2014 The Saccharomyces cerevisiae Aft1 and Kluyveromyces lactis KlAft are orthologous yeast transcription activators that regulate the expression of the same group of iron-uptake genes but bind to the different DNA sites: TGCACCC for Aft1 and PuCACCC for KlAft. Iron 162-166 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 29-33 24591629-1 2014 The paralogous iron-responsive transcription factors Aft1 and Aft2 (activators of ferrous transport) regulate iron homeostasis in Saccharomyces cerevisiae by activating expression of iron-uptake and -transport genes when intracellular iron is low. Iron 15-19 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 53-57 24591629-1 2014 The paralogous iron-responsive transcription factors Aft1 and Aft2 (activators of ferrous transport) regulate iron homeostasis in Saccharomyces cerevisiae by activating expression of iron-uptake and -transport genes when intracellular iron is low. Iron 110-114 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 53-57 24591629-1 2014 The paralogous iron-responsive transcription factors Aft1 and Aft2 (activators of ferrous transport) regulate iron homeostasis in Saccharomyces cerevisiae by activating expression of iron-uptake and -transport genes when intracellular iron is low. Iron 110-114 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 53-57 24591629-1 2014 The paralogous iron-responsive transcription factors Aft1 and Aft2 (activators of ferrous transport) regulate iron homeostasis in Saccharomyces cerevisiae by activating expression of iron-uptake and -transport genes when intracellular iron is low. Iron 110-114 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 53-57 24172132-1 2014 The Saccharomyces cerevisiae Aft1 and Kluyveromyces lactis KlAft are orthologous yeast transcription activators that regulate the expression of the same group of iron-uptake genes but bind to the different DNA sites: TGCACCC for Aft1 and PuCACCC for KlAft. Iron 162-166 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 229-233 24172132-4 2014 We show with deletion mutant analyses that this sequence is essential for the binding of Aft1 to its DNA site and for the iron uptake and growth of S. cerevisiae under iron-limited conditions. Iron 122-126 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 89-93 24172132-4 2014 We show with deletion mutant analyses that this sequence is essential for the binding of Aft1 to its DNA site and for the iron uptake and growth of S. cerevisiae under iron-limited conditions. Iron 168-172 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 89-93 24172132-6 2014 We show that the Aft1 region is necessary and sufficient for KlAft to bind efficiently to the Aft1 DNA site in S. cerevisiae and to complement the iron-dependent phenotype of the aft1Deltaaft2Delta mutant. Iron 147-151 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 17-21 23295455-3 2013 Here we report that arsenic activates the iron responsive transcription factor, Aft1, as a consequence of a defective high-affinity iron uptake mediated by Fet3 and Ftr1, whose mRNAs are drastically decreased upon arsenic exposure. Iron 132-136 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 80-84 24142925-6 2013 Molecular validation experiments pinpointed the iron metabolism factors AFT1, CCC1, and YAP5 as contributors to these molecular and cellular phenotypes; in genome-scale sequence analyses, a suite of iron toxicity response genes showed evidence for rapid protein evolution in Malaysian yeast. Iron 48-52 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 72-76 24142925-6 2013 Molecular validation experiments pinpointed the iron metabolism factors AFT1, CCC1, and YAP5 as contributors to these molecular and cellular phenotypes; in genome-scale sequence analyses, a suite of iron toxicity response genes showed evidence for rapid protein evolution in Malaysian yeast. Iron 199-203 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 72-76 23962819-1 2013 Regulation of iron metabolism in Saccharomyces cerevisiae is achieved at the transcriptional level by low (Aft1 and Aft2) and high iron-sensing (Yap5) transcription factors, and at the post-transcriptional level by mRNA-binding proteins (Cth1 and Cth2). Iron 14-18 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 107-111 23295455-3 2013 Here we report that arsenic activates the iron responsive transcription factor, Aft1, as a consequence of a defective high-affinity iron uptake mediated by Fet3 and Ftr1, whose mRNAs are drastically decreased upon arsenic exposure. Iron 42-46 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 80-84 23962819-2 2013 In this review we highlight recent studies unveiling the critical role that iron-sulfur clusters play in control of Aft1/2 and Yap5 activity, as well as the complex relationship between iron homeostasis and thiol redox metabolism. Iron 76-80 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 116-122 24074273-8 2013 Deletion of either of the iron homeostasis regulators AFT1 or AFT2 also resulted in sensitivity to LoaOOH. Iron 26-30 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 54-58 23864114-6 2013 Genome wide transcriptional profiling revealed a constitutive activation of the iron regulon that could be accounted for by a constitutive nuclear localization of the transcriptional activator Aft1. Iron 80-84 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 193-197 23457300-3 2013 A previous microarray study in a vma mutant demonstrated up-regulation of multiple iron uptake genes under control of Aft1p (the iron regulon) and only one antioxidant gene, the peroxiredoxin TSA2 (Milgrom, E., Diab, H., Middleton, F., and Kane, P. M. (2007) Loss of vacuolar proton-translocating ATPase activity in yeast results in chronic oxidative stress. Iron 83-87 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 118-123 22157760-0 2012 Iron-responsive transcription factor Aft1 interacts with kinetochore protein Iml3 and promotes pericentromeric cohesin. Iron 0-4 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 37-41 23045394-0 2012 Iron-induced dissociation of the Aft1p transcriptional regulator from target gene promoters is an initial event in iron-dependent gene suppression. Iron 0-4 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 33-38 23045394-0 2012 Iron-induced dissociation of the Aft1p transcriptional regulator from target gene promoters is an initial event in iron-dependent gene suppression. Iron 115-119 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 33-38 23045394-1 2012 Aft1p is an iron-responsive transcriptional activator that plays a central role in the regulation of iron metabolism in Saccharomyces cerevisiae. Iron 12-16 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 0-5 23045394-1 2012 Aft1p is an iron-responsive transcriptional activator that plays a central role in the regulation of iron metabolism in Saccharomyces cerevisiae. Iron 101-105 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 0-5 23045394-2 2012 Aft1p is regulated by accelerated nuclear export in the presence of iron, mediated by Msn5p. Iron 68-72 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 0-5 23045394-3 2012 However, the transcriptional activity of Aft1p is suppressed under iron-replete conditions in the Deltamsn5 strain, although Aft1p remains in the nucleus. Iron 67-71 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 41-46 23045394-4 2012 Aft1p dissociates from its target promoters under iron-replete conditions due to an interaction between Aft1p and the monothiol glutaredoxin Grx3p or Grx4p (Grx3/4p). Iron 50-54 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 0-5 23045394-4 2012 Aft1p dissociates from its target promoters under iron-replete conditions due to an interaction between Aft1p and the monothiol glutaredoxin Grx3p or Grx4p (Grx3/4p). Iron 50-54 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 104-109 23045394-5 2012 The binding of Grx3/4p to Aft1p is induced by iron repletion and requires binding of an iron-sulfur cluster to Grx3/4p. Iron 46-50 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 26-31 23045394-5 2012 The binding of Grx3/4p to Aft1p is induced by iron repletion and requires binding of an iron-sulfur cluster to Grx3/4p. Iron 88-92 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 26-31 23045394-6 2012 The mitochondrial transporter Atm1p, which has been implicated in the export of iron-sulfur clusters and related molecules, is required not only for iron binding to Grx3p but also for dissociation of Aft1p from its target promoters. Iron 80-84 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 200-205 23045394-7 2012 These results suggest that iron binding to Grx3p (and presumably Grx4p) is a prerequisite for the suppression of Aft1p. Iron 27-31 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 113-118 22683637-4 2012 The upregulation of CTR2 induced by iron depletion was abrogated by the genetic deletion of either Mac1p or iron-sensing transcription factor Aft1p. Iron 36-40 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 142-147 22157760-1 2012 The Saccharomyces cerevisiae iron-responsive transcription factor, Aft1, has a well established role in regulating iron homeostasis through the transcriptional induction of iron-regulon genes. Iron 29-33 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 67-71 22157760-1 2012 The Saccharomyces cerevisiae iron-responsive transcription factor, Aft1, has a well established role in regulating iron homeostasis through the transcriptional induction of iron-regulon genes. Iron 115-119 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 67-71 22157760-1 2012 The Saccharomyces cerevisiae iron-responsive transcription factor, Aft1, has a well established role in regulating iron homeostasis through the transcriptional induction of iron-regulon genes. Iron 115-119 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 67-71 21361388-0 2011 Biophysical investigation of the iron in Aft1-1(up) and Gal-YAH1 Saccharomyces cerevisiae. Iron 33-37 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 41-45 21299470-8 2011 Grx4 functions as iron sensor for the iron-sensing transcription factor Aft1 in S. cerevisiae. Iron 18-22 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 72-76 21299470-8 2011 Grx4 functions as iron sensor for the iron-sensing transcription factor Aft1 in S. cerevisiae. Iron 38-42 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 72-76 21385868-8 2011 Genes of the Aft1p-dependent iron regulon were induced specifically in the absence of Prx1p despite optimal mitochondrial Fe-S biogenesis, suggesting dysfunction of the mitochondria to the cytosol signaling pathway. Iron 29-33 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 13-18 21385868-8 2011 Genes of the Aft1p-dependent iron regulon were induced specifically in the absence of Prx1p despite optimal mitochondrial Fe-S biogenesis, suggesting dysfunction of the mitochondria to the cytosol signaling pathway. Iron 122-126 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 13-18 21361388-1 2011 Aft1p is a major iron regulator in budding yeast Saccharomyces cerevisiae. Iron 17-21 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 0-5 21361388-3 2011 Aft1p within the Aft1-1(up) strain has a single amino acid mutation which causes it to constitutively activate iron regulon genes regardless of cellular Fe status. Iron 111-115 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 0-5 21361388-3 2011 Aft1p within the Aft1-1(up) strain has a single amino acid mutation which causes it to constitutively activate iron regulon genes regardless of cellular Fe status. Iron 111-115 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 0-4 21361388-3 2011 Aft1p within the Aft1-1(up) strain has a single amino acid mutation which causes it to constitutively activate iron regulon genes regardless of cellular Fe status. Iron 153-155 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 0-5 21361388-3 2011 Aft1p within the Aft1-1(up) strain has a single amino acid mutation which causes it to constitutively activate iron regulon genes regardless of cellular Fe status. Iron 153-155 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 0-4 21361388-5 2011 Ferredoxin Yah1p is involved in Fe/S cluster assembly, and Aft1p-targeted iron regulon genes are also upregulated in Yah1p-depleted cells. Iron 74-78 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 59-64 21361388-6 2011 In this study Mossbauer, EPR, and UV-vis spectroscopies were used to characterize the Fe distribution in Aft1-1(up) and Yah1p-depleted cells. Iron 86-88 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 105-109 21361388-7 2011 Aft1-1(up) cells grown in low Fe medium contained more Fe than did WT cells. Iron 30-32 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 0-4 21361388-8 2011 A basal level of Fe in both WT and Aft1-1(up) cells was located in mitochondria, primarily in the form of Fe/S clusters and heme centers. Iron 17-19 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 35-39 21361388-8 2011 A basal level of Fe in both WT and Aft1-1(up) cells was located in mitochondria, primarily in the form of Fe/S clusters and heme centers. Iron 106-108 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 35-39 21361388-11 2011 Aft1-1(up) cells grown in high Fe medium contained far more Fe than found in WT cells. Iron 31-33 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 0-4 21361388-11 2011 Aft1-1(up) cells grown in high Fe medium contained far more Fe than found in WT cells. Iron 60-62 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 0-4 20978135-2 2011 Genetic studies indicate that Grx3/4 and Fra2 regulate activity of the iron-responsive transcription factors Aft1 and Aft2 in response to mitochondrial Fe-S cluster biosynthesis. Iron 71-75 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 109-113 21131439-4 2011 There are three types of iron-responsive transcriptional regulators in fungi; Aft1/Aft2 activators in yeast, GATA-type repressors in many fungi, and HapX/Php4 in Schizosaccharomyces pombe and Aspergillus species. Iron 25-29 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 78-82 20978135-2 2011 Genetic studies indicate that Grx3/4 and Fra2 regulate activity of the iron-responsive transcription factors Aft1 and Aft2 in response to mitochondrial Fe-S cluster biosynthesis. Iron 152-156 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 109-113 20978135-4 2011 To further characterize this unusual Fe-S-binding complex, site-directed mutagenesis was used to identify specific residues in Fra2 that influence Fe-S cluster binding and regulation of Aft1 activity in vivo. Iron 37-41 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 186-190 20978135-7 2011 In vivo genetic studies further confirm that Fra2 His-103 is critical for control of Aft1 activity in response to the cellular iron status. Iron 127-131 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 85-89 20695822-5 2010 Interestingly, the Aft1-dependent iron regulon is a main target of PDTC, indicating a lack of iron availability. Iron 34-38 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 19-23 20695822-9 2010 Hence, we show that PDTC induces cytoplasmic zinc excess, eliciting vacuolar detoxification, which in turn, disturbs iron homeostasis and activates the iron-dependent regulator Aft1. Iron 152-156 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 177-181 19715344-3 2009 However, the interactions between these proteins and the iron-dependent mechanism by which they control Aft1 localization are unclear. Iron 57-61 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 104-108 20429018-8 2010 The iron-responsive transcription factor Aft1p appears activated in cells with high phosphate content in spite of normal intracellular iron levels. Iron 4-8 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 41-46 20429018-8 2010 The iron-responsive transcription factor Aft1p appears activated in cells with high phosphate content in spite of normal intracellular iron levels. Iron 135-139 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 41-46 20439772-0 2010 Functional genomics analysis of the Saccharomyces cerevisiae iron responsive transcription factor Aft1 reveals iron-independent functions. Iron 61-65 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 98-102 20439772-0 2010 Functional genomics analysis of the Saccharomyces cerevisiae iron responsive transcription factor Aft1 reveals iron-independent functions. Iron 111-115 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 98-102 20439772-1 2010 The Saccharomyces cerevisiae transcription factor Aft1 is activated in iron-deficient cells to induce the expression of iron regulon genes, which coordinate the increase of iron uptake and remodel cellular metabolism to survive low-iron conditions. Iron 71-75 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 50-54 20439772-1 2010 The Saccharomyces cerevisiae transcription factor Aft1 is activated in iron-deficient cells to induce the expression of iron regulon genes, which coordinate the increase of iron uptake and remodel cellular metabolism to survive low-iron conditions. Iron 120-124 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 50-54 20439772-1 2010 The Saccharomyces cerevisiae transcription factor Aft1 is activated in iron-deficient cells to induce the expression of iron regulon genes, which coordinate the increase of iron uptake and remodel cellular metabolism to survive low-iron conditions. Iron 120-124 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 50-54 20439772-1 2010 The Saccharomyces cerevisiae transcription factor Aft1 is activated in iron-deficient cells to induce the expression of iron regulon genes, which coordinate the increase of iron uptake and remodel cellular metabolism to survive low-iron conditions. Iron 120-124 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 50-54 20439772-6 2010 We demonstrate that Aft1 works in parallel with the RIM101 pH pathway and the role of Aft1 in DNA damage repair is mediated by iron. Iron 127-131 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 86-90 19715344-1 2009 The transcription of iron uptake and storage genes in Saccharomyces cerevisiae is primarily regulated by the transcription factor Aft1. Iron 21-25 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 130-134 19715344-2 2009 Nucleocytoplasmic shuttling of Aft1 is dependent upon mitochondrial Fe-S cluster biosynthesis via a signaling pathway that includes the cytosolic monothiol glutaredoxins (Grx3 and Grx4) and the BolA homologue Fra2. Iron 68-70 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 31-35 19581449-0 2009 KlAft, the Kluyveromyces lactis ortholog of Aft1 and Aft2, mediates activation of iron-responsive transcription through the PuCACCC Aft-type sequence. Iron 82-86 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 44-48 19581449-5 2009 We identify an ortholog of AFT1/AFT2, designated KlAFT, whose deletion leads to the inability to grow under iron limitation. Iron 108-112 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 27-31 19581449-6 2009 We show with quantitative real-time PCR analysis that KlAft activates the transcription of all homologs of the Aft1-target genes involved in the iron transport at the cell surface in response to iron limitation. Iron 145-149 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 111-115 19581449-6 2009 We show with quantitative real-time PCR analysis that KlAft activates the transcription of all homologs of the Aft1-target genes involved in the iron transport at the cell surface in response to iron limitation. Iron 195-199 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 111-115 19469713-1 2009 Aft1 is a transcriptional activator in Saccharomyces cerevisiae that responds to iron availability and regulates the expression of genes in the iron regulon, such as FET3, FTR1 and the ARN family. Iron 81-85 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 0-4 19469713-1 2009 Aft1 is a transcriptional activator in Saccharomyces cerevisiae that responds to iron availability and regulates the expression of genes in the iron regulon, such as FET3, FTR1 and the ARN family. Iron 144-148 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 0-4 18710911-9 2008 The effects of cryptolepine on the transcription of iron transport-related genes were consistent with a loss of function of the iron sensor Aft1p, indicating a possible disruption of iron metabolism in S. cerevisiae. Iron 128-132 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 140-145 18710911-9 2008 The effects of cryptolepine on the transcription of iron transport-related genes were consistent with a loss of function of the iron sensor Aft1p, indicating a possible disruption of iron metabolism in S. cerevisiae. Iron 128-132 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 140-145 17415523-5 2007 Two S. cerevisiae monothiol glutaredoxins with the thioredoxin-like extension, Grx3 and Grx4, are modulators of the transcriptional activator Aft1, which regulates iron uptake in yeast. Iron 164-168 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 142-146 18227070-7 2008 First, ISC depletion activates the iron-responsive transcription factors Aft1/2p leading to increased cellular iron acquisition. Iron 35-39 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 73-80 18227070-7 2008 First, ISC depletion activates the iron-responsive transcription factors Aft1/2p leading to increased cellular iron acquisition. Iron 111-115 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 73-80 17538022-5 2007 Phosphorylation of S210 and S224 in Aft1p, which is not iron dependent, and the iron-induced intermolecular interaction of Aft1p are both essential for its recognition by Msn5p. Iron 80-84 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 123-128 17538022-7 2007 Collectively, these results suggest that iron induces a conformational change in Aft1p, in which Aft1p Cys291 plays a critical role, and that, in turn, Aft1p is recognized by Msn5p and exported into the cytoplasm in an iron-dependent manner. Iron 41-45 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 81-86 17538022-7 2007 Collectively, these results suggest that iron induces a conformational change in Aft1p, in which Aft1p Cys291 plays a critical role, and that, in turn, Aft1p is recognized by Msn5p and exported into the cytoplasm in an iron-dependent manner. Iron 41-45 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 97-102 17538022-7 2007 Collectively, these results suggest that iron induces a conformational change in Aft1p, in which Aft1p Cys291 plays a critical role, and that, in turn, Aft1p is recognized by Msn5p and exported into the cytoplasm in an iron-dependent manner. Iron 41-45 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 97-102 17538022-7 2007 Collectively, these results suggest that iron induces a conformational change in Aft1p, in which Aft1p Cys291 plays a critical role, and that, in turn, Aft1p is recognized by Msn5p and exported into the cytoplasm in an iron-dependent manner. Iron 219-223 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 81-86 17538022-7 2007 Collectively, these results suggest that iron induces a conformational change in Aft1p, in which Aft1p Cys291 plays a critical role, and that, in turn, Aft1p is recognized by Msn5p and exported into the cytoplasm in an iron-dependent manner. Iron 219-223 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 97-102 17538022-7 2007 Collectively, these results suggest that iron induces a conformational change in Aft1p, in which Aft1p Cys291 plays a critical role, and that, in turn, Aft1p is recognized by Msn5p and exported into the cytoplasm in an iron-dependent manner. Iron 219-223 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 97-102 17630978-5 2007 Interestingly, a mutant lacking the Aft1 transcription factor, required for the transcriptional response to iron starvation, was found to be highly sensitive to zinc. Iron 108-112 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 36-40 18703840-1 2008 Yeast glutaredoxin 3 (Grx3) is a cytosolic protein that regulates the activity of the iron-responsive transcriptional activator Aft1. Iron 86-90 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 128-132 18281282-1 2008 The nature of the connection between mitochondrial Fe-S cluster synthesis and the iron-sensitive transcription factor Aft1 in regulating the expression of the iron transport system in Saccharomyces cerevisiae is not known. Iron 82-86 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 118-122 18281282-1 2008 The nature of the connection between mitochondrial Fe-S cluster synthesis and the iron-sensitive transcription factor Aft1 in regulating the expression of the iron transport system in Saccharomyces cerevisiae is not known. Iron 159-163 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 118-122 18281282-4 2008 In cells incubated in high iron medium, deletion of either FRA gene results in the translocation of the low iron-sensing transcription factor Aft1 into the nucleus, where it occupies the FET3 promoter. Iron 27-31 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 142-146 18281282-4 2008 In cells incubated in high iron medium, deletion of either FRA gene results in the translocation of the low iron-sensing transcription factor Aft1 into the nucleus, where it occupies the FET3 promoter. Iron 108-112 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 142-146 17538022-0 2007 Mechanism underlying the iron-dependent nuclear export of the iron-responsive transcription factor Aft1p in Saccharomyces cerevisiae. Iron 25-29 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 99-104 17538022-0 2007 Mechanism underlying the iron-dependent nuclear export of the iron-responsive transcription factor Aft1p in Saccharomyces cerevisiae. Iron 62-66 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 99-104 17538022-1 2007 Aft1p is an iron-responsive transcriptional activator that plays a central role in maintaining iron homeostasis in Saccharomyces cerevisiae. Iron 12-16 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 0-5 17538022-1 2007 Aft1p is an iron-responsive transcriptional activator that plays a central role in maintaining iron homeostasis in Saccharomyces cerevisiae. Iron 95-99 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 0-5 17538022-2 2007 Aft1p is regulated primarily by iron-induced shuttling of the protein between the nucleus and cytoplasm, but its nuclear import is not regulated by iron. Iron 32-36 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 0-5 17538022-3 2007 Here, we have shown that the nuclear export of Aft1p is promoted in the presence of iron and that Msn5p is the nuclear export receptor (exportin) for Aft1p. Iron 84-88 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 47-52 17538022-4 2007 Msn5p recognizes Aft1p in the iron-replete condition. Iron 30-34 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 17-22 17096368-0 2007 Cisplatin upregulates Saccharomyces cerevisiae genes involved in iron homeostasis through activation of the iron insufficiency-responsive transcription factor Aft1. Iron 65-69 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 159-163 17215245-11 2007 Microarray analysis comparing wild-type and vma2Delta mutant cells grown at pH 5, permissive conditions for the vma2Delta mutant, indicated high level up-regulation of several iron uptake and metabolism genes that are part of the Aft1/Aft2 regulon. Iron 176-180 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 230-234 17096368-0 2007 Cisplatin upregulates Saccharomyces cerevisiae genes involved in iron homeostasis through activation of the iron insufficiency-responsive transcription factor Aft1. Iron 108-112 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 159-163 17096368-3 2007 Interestingly, the expression of all 14 genes is known to be regulated by Aft1, a transcription factor activated in response to iron insufficiency. Iron 128-132 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 74-78 17096368-5 2007 The active domain of Aft1 necessary for activation of the FET3 promoter by cisplatin is identical to the one required for activation by bathophenanthroline sulfonate, an inhibitor of cellular iron uptake. Iron 192-196 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 21-25 17096368-7 2007 These findings suggest that cisplatin activates Aft1 through the inhibition of iron uptake into the cells, after which the expression of Aft1 target genes involved in iron uptake might be induced. Iron 79-83 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 48-52 17096368-7 2007 These findings suggest that cisplatin activates Aft1 through the inhibition of iron uptake into the cells, after which the expression of Aft1 target genes involved in iron uptake might be induced. Iron 79-83 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 137-141 17096368-7 2007 These findings suggest that cisplatin activates Aft1 through the inhibition of iron uptake into the cells, after which the expression of Aft1 target genes involved in iron uptake might be induced. Iron 167-171 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 48-52 17096368-7 2007 These findings suggest that cisplatin activates Aft1 through the inhibition of iron uptake into the cells, after which the expression of Aft1 target genes involved in iron uptake might be induced. Iron 167-171 DNA-binding transcription factor AFT1 Saccharomyces cerevisiae S288C 137-141