PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 2470733-4 1989 For each agonist tested (histamine, thrombin, phorbol 12-myristate 13-acetate, and the calcium ionophore A23187) a dose-dependent redistribution of GMP-140 to the endothelial surface was observed which closely paralleled the dose-dependent secretion of vWF into the cell supernatant. Calcimycin 105-111 von Willebrand factor Homo sapiens 253-256 2786433-7 1989 Calcium ionophore A23187 increased the amount of the large vWF multimer released into the culture media, but did not change its multimeric composition. Calcimycin 18-24 von Willebrand factor Homo sapiens 59-62 2107883-3 1990 Culture medium containing constitutively secreted vWF was removed from metabolically labeled primary cultures of human umbilical vein EC, and vWF released from WPB was obtained after stimulation by A23187. Calcimycin 198-204 von Willebrand factor Homo sapiens 142-145 2107883-7 1990 Multimers of greater than 20 subunits represented 60% +/- 4% (SEM) of A23187 released vWF and 11% +/- 5% of media vWF, but binding to fibrin was similar, 96% +/- 1% and 94% +/- 2%, respectively. Calcimycin 70-76 von Willebrand factor Homo sapiens 86-89 2107883-8 1990 A progressively smaller proportion of vWF bound as multimer size decreased, and dimeric vWF binding was least, with 34% +/- 5% binding from A23187 releasate and 51% +/- 4% from media. Calcimycin 140-146 von Willebrand factor Homo sapiens 88-91 2494192-16 1989 On the contrary, an average of 90% of vWf released from Weibel-Palade bodies after treatment with the calcium ionophore A23187 or PMA appeared in the basolateral chamber, indicating that the regulated pathway of secretion is highly polarized. Calcimycin 120-126 von Willebrand factor Homo sapiens 38-41 3105624-3 1987 Increased binding of A23187-released vWf was not due to another component present in the releasate, since releasate from which vWf was adsorbed, when added together with constitutively secreted vWf, did not promote binding. Calcimycin 21-27 von Willebrand factor Homo sapiens 37-40 2822171-6 1987 Washed platelets in a protein-free buffer activated by 1 mumol/L calcium ionophore A23187 or 10 mumol/L ADP also bound vWf, suggesting that we were detecting surface binding of alpha-granule-derived vWf. Calcimycin 83-89 von Willebrand factor Homo sapiens 119-122 2822171-6 1987 Washed platelets in a protein-free buffer activated by 1 mumol/L calcium ionophore A23187 or 10 mumol/L ADP also bound vWf, suggesting that we were detecting surface binding of alpha-granule-derived vWf. Calcimycin 83-89 von Willebrand factor Homo sapiens 199-202 3305020-3 1987 Stimulation of these cells with secretagogues (A23187, thrombin) produced only 30% release of vWf in comparison to control cells containing intact microtubules. Calcimycin 47-53 von Willebrand factor Homo sapiens 94-97 3105624-3 1987 Increased binding of A23187-released vWf was not due to another component present in the releasate, since releasate from which vWf was adsorbed, when added together with constitutively secreted vWf, did not promote binding. Calcimycin 21-27 von Willebrand factor Homo sapiens 127-130 3105624-3 1987 Increased binding of A23187-released vWf was not due to another component present in the releasate, since releasate from which vWf was adsorbed, when added together with constitutively secreted vWf, did not promote binding. Calcimycin 21-27 von Willebrand factor Homo sapiens 127-130 2421805-5 1986 The PMN cell enzyme responsible for vWF:Ag fragmentation is located intracellularly and released by freezethaw lysis or cell activation by calcium or the calcium ionophore A23187. Calcimycin 172-178 von Willebrand factor Homo sapiens 36-39 3087627-3 1986 In contrast, the vWf released by the calcium ionophore A23187 or thrombin consisted of only very large multimers of mature subunits. Calcimycin 55-61 von Willebrand factor Homo sapiens 17-20 6435280-1 1984 Activation of washed platelets in the presence of EDTA with either 1 U/ml of alpha-thrombin or 2 microM calcium ionophore (A23187) caused the release of one-third to one-half of the platelet factor VIII/von Willebrand factor (FVIII/vWF) into the supernatant. Calcimycin 123-129 von Willebrand factor Homo sapiens 232-235 6436246-5 1984 The phorbol ester 4 beta-phorbol 12-myristate 13-acetate (PMA) and the Ca2+ ionophore A23187 both bypass the phospholipid methylation and directly stimulate Ca2+ influx and von Willebrand factor release. Calcimycin 86-92 von Willebrand factor Homo sapiens 173-194 6087354-5 1984 A purine nucleotide affinity analog, 5"-p-fluorosulfonylbenzoyl adenosine (FSBA), which covalently modifies the ADP binding sites on the human platelet membrane, prevented binding of vWF induced with ADP, as well as with human thrombin and with ionophore A23187, agents known to cause platelet ADP secretion. Calcimycin 255-261 von Willebrand factor Homo sapiens 183-186 11456068-4 2001 After 1 day of culture, both basal and A23187-stimulated secretion of vWF (expressed per 1,000,000 cells) was considerably increased at low cell densities (i.e. below 5000 cells/cm2) on all substrates. Calcimycin 39-45 von Willebrand factor Homo sapiens 70-73 12445472-4 2002 We demonstrate that vWF and tPA which are stored in different granules within endothelial cells are released with different kinetics following endothelial stimulation with histamine or the Ca(2+) ionophore A23187. Calcimycin 206-212 von Willebrand factor Homo sapiens 20-23 10634936-6 2000 Acute vWf release occurred in response to the calcium ionophore A23187. Calcimycin 64-70 von Willebrand factor Homo sapiens 6-9 8218102-4 1993 A larger portion of the vWf-adherent platelet membranes (approximately 21%) was released as microvesicles subsequent to platelet stimulation with the nonphysiological agonist calcium ionophore A23187. Calcimycin 193-199 von Willebrand factor Homo sapiens 24-27 8249125-6 1993 However, at therapeutic concentrations (0.1-5 micrograms/ml) vWF release by cells stimulated with thrombin, histamine, PMA, and the calcium ionophore A23187 was enhanced by both CsA and cremophor in a concentration-dependent manner. Calcimycin 150-156 von Willebrand factor Homo sapiens 61-64