PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 7548083-0 1995 N-terminus and lysine side chain pKa values of melittin in aqueous solutions and micellar dispersions measured by 15N NMR. 15n 114-117 melittin Apis mellifera 47-55 29668274-5 2018 Here we present high-resolution nuclear magnetic resonance studies in the lower-concentration regime using a novel method to produce isotope-labeled (15N and 13C) recombinant melittin. 15n 150-153 melittin Apis mellifera 175-183 7548083-5 1995 In the present study, high-resolution 15N NMR at 50.6 MHz was used to directly measure the pKa values of the amino groups of the Gly-1, Lys-7, Lys-21, and Lys-23 residues of MLT. 15n 38-41 melittin Apis mellifera 174-177 7548083-6 1995 Specifically, the pH dependence of MLT 15N chemical shifts was measured separately for the isotopically enriched backbone nitrogen of Gly-1 and the side chain nitrogen atoms of Lys-7, Lys-21, and Lys-23 at a MLT concentration of 1.2 mM and a temperature of 23 degrees C. Measurements were made for MLT in potassium phosphate buffer, in neat water, and in 1-myristoyl-2-hydroxyl-sn-glycero-3-phosphocholine (MMPC) lipid micelles. 15n 39-42 melittin Apis mellifera 35-38