PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 27189364-0 2016 Three Candida albicans potassium uptake systems differ in their ability to provide Saccharomyces cerevisiae trk1trk2 mutants with necessary potassium. Potassium 23-32 Trk1p Saccharomyces cerevisiae S288C 108-116 27895122-2 2017 Among the major determinants of potassium uptake in the model organism Saccharomyces cerevisiae are the Trk1 high affinity potassium transporter and the functionally redundant Hal4 (Sat4) and Hal5 protein kinases. Potassium 32-41 Trk1p Saccharomyces cerevisiae S288C 104-108 27895122-6 2017 Specifically, growth under limiting potassium alters the activities of Npr1 and another TORC1 effector kinase, Sch9; hal4 hal5 and trk1 trk2 mutants display hypersensitivity to rapamycin, and reciprocally, TORC1 inhibition reduces potassium accumulation. Potassium 36-45 Trk1p Saccharomyces cerevisiae S288C 131-135 27189364-0 2016 Three Candida albicans potassium uptake systems differ in their ability to provide Saccharomyces cerevisiae trk1trk2 mutants with necessary potassium. Potassium 140-149 Trk1p Saccharomyces cerevisiae S288C 108-116 25777080-0 2015 A physiological, biochemical and proteomic characterization of Saccharomyces cerevisiae trk1,2 transport mutants grown under limiting potassium conditions. Potassium 134-143 Trk1p Saccharomyces cerevisiae S288C 88-92 27058598-0 2016 Potassium Uptake Mediated by Trk1 Is Crucial for Candida glabrata Growth and Fitness. Potassium 0-9 Trk1p Saccharomyces cerevisiae S288C 29-33 27058598-2 2016 Three types of plasma-membrane systems mediating potassium influx with different transport mechanisms have been described in yeasts: the Trk1 uniporter, the Hak cation-proton symporter and the Acu ATPase. Potassium 49-58 Trk1p Saccharomyces cerevisiae S288C 137-141 27058598-3 2016 The C. glabrata genome contains only one gene encoding putative system for potassium uptake, the Trk1 uniporter. Potassium 75-84 Trk1p Saccharomyces cerevisiae S288C 97-101 25777080-4 2015 Using a combination of physiological, biochemical and proteomic approaches, we show that during growth at suboptimal potassium concentrations, double trk1,2 mutants accumulate less potassium and reach lower yields. Potassium 117-126 Trk1p Saccharomyces cerevisiae S288C 150-154 25777080-4 2015 Using a combination of physiological, biochemical and proteomic approaches, we show that during growth at suboptimal potassium concentrations, double trk1,2 mutants accumulate less potassium and reach lower yields. Potassium 181-190 Trk1p Saccharomyces cerevisiae S288C 150-154 25687974-1 2015 Potassium ion (K+) uptake in yeast is mediated mainly by the Trk1/2 proteins that enable cells to survive on external K+ concentration as low as a few muM. Potassium 0-9 Trk1p Saccharomyces cerevisiae S288C 61-67 23454581-5 2013 The evidence indicates that hyperactivation of Ppz1 would impair potassium transport through the Trk1/Trk2 transporters, thus resulting in a decrease in the intracellular pH and a subsequent increase in the levels of cAMP. Potassium 65-74 Trk1p Saccharomyces cerevisiae S288C 97-101 24052423-4 2013 Whereas the wild type and the tok1 mutant cells exhibited similar depolarization curves, mutant cells lacking the two Trk1,2 potassium transporters revealed a significantly decreased membrane depolarization by K(+), particularly at lower extracellular potassium concentration [K(+)]out. Potassium 126-135 Trk1p Saccharomyces cerevisiae S288C 119-123 23454581-8 2013 Cells lacking Trk1,2 display an invasive phenotype that is abolished by deletion of FLO8 or by increasing the potassium concentration in the medium. Potassium 110-119 Trk1p Saccharomyces cerevisiae S288C 14-18 24021239-0 2014 Role of Saccharomyces cerevisiae Trk1 in stabilization of intracellular potassium content upon changes in external potassium levels. Potassium 72-81 Trk1p Saccharomyces cerevisiae S288C 33-37 24021239-0 2014 Role of Saccharomyces cerevisiae Trk1 in stabilization of intracellular potassium content upon changes in external potassium levels. Potassium 115-124 Trk1p Saccharomyces cerevisiae S288C 33-37 24021239-3 2014 By using yeasts lacking the Trk1,2 system or expressing different versions of the mutated main plasma membrane potassium transporter (Trk1), we show that Trk1 is not essential for adaptation to potassium changes but the dynamics of potassium loss is very different in the wild type and in trk1,2 mutant or in yeasts expressing Trk1 versions with highly impaired transport characteristics. Potassium 111-120 Trk1p Saccharomyces cerevisiae S288C 134-138 24021239-3 2014 By using yeasts lacking the Trk1,2 system or expressing different versions of the mutated main plasma membrane potassium transporter (Trk1), we show that Trk1 is not essential for adaptation to potassium changes but the dynamics of potassium loss is very different in the wild type and in trk1,2 mutant or in yeasts expressing Trk1 versions with highly impaired transport characteristics. Potassium 111-120 Trk1p Saccharomyces cerevisiae S288C 134-138 24021239-3 2014 By using yeasts lacking the Trk1,2 system or expressing different versions of the mutated main plasma membrane potassium transporter (Trk1), we show that Trk1 is not essential for adaptation to potassium changes but the dynamics of potassium loss is very different in the wild type and in trk1,2 mutant or in yeasts expressing Trk1 versions with highly impaired transport characteristics. Potassium 111-120 Trk1p Saccharomyces cerevisiae S288C 289-293 24021239-3 2014 By using yeasts lacking the Trk1,2 system or expressing different versions of the mutated main plasma membrane potassium transporter (Trk1), we show that Trk1 is not essential for adaptation to potassium changes but the dynamics of potassium loss is very different in the wild type and in trk1,2 mutant or in yeasts expressing Trk1 versions with highly impaired transport characteristics. Potassium 111-120 Trk1p Saccharomyces cerevisiae S288C 134-138 24267958-3 2014 In the model yeast Saccharomyces cerevisiae, two uptake systems, Trk1 and Trk2, are responsible for the accumulation of a relatively high intracellular potassium content (200-300 mM) and the efflux of surplus potassium is mediated by the Tok1 channel and active exporters Ena ATPase and Nha1 cation/proton antiporter. Potassium 152-161 Trk1p Saccharomyces cerevisiae S288C 65-69 24267958-3 2014 In the model yeast Saccharomyces cerevisiae, two uptake systems, Trk1 and Trk2, are responsible for the accumulation of a relatively high intracellular potassium content (200-300 mM) and the efflux of surplus potassium is mediated by the Tok1 channel and active exporters Ena ATPase and Nha1 cation/proton antiporter. Potassium 209-218 Trk1p Saccharomyces cerevisiae S288C 65-69 23550140-5 2013 This gene encodes a transporter required for high-affinity potassium transport in S. cerevisiae Data from reciprocal hemizygosity experiments with TRK1 deletion strains in K12 and BY backgrounds, as well as analysis of the deletion of this gene in the K12 strain, demonstrate that the K12 allele of TRK1 is responsible for ammonium toxicity resistance. Potassium 59-68 Trk1p Saccharomyces cerevisiae S288C 299-303 23550140-7 2013 These results demonstrate that the gene encoded by the K12 allele of TRK1 has a greater affinity for potassium than the standard allele of TRK1 found in Saccharomyces strains. Potassium 101-110 Trk1p Saccharomyces cerevisiae S288C 69-73 23039231-4 2012 Lack of potassium drastically alters sulfur metabolism (mainly Met and Cys metabolism), triggers an oxidative stress response and activates the retrograde pathway, possibly due to the ammonium accumulation that occurs through the Trk1 potassium transporter. Potassium 8-17 Trk1p Saccharomyces cerevisiae S288C 230-234 22948499-6 2012 In contrast to S. cerevisiae, Z. rouxii cells without the TRK1 gene contained less potassium than the control cells and their plasma membrane was significantly hyperpolarized compared with those of the parental strain when grown in the presence of 100 mM KCl. Potassium 83-92 Trk1p Saccharomyces cerevisiae S288C 58-62 23039231-6 2012 Only specific subsets of these changes were observed in a strain deleted for the TRK1 and TRK2 genes growing in the presence of sufficient potassium (50 mM). Potassium 139-148 Trk1p Saccharomyces cerevisiae S288C 81-85 16358319-7 2005 The use of a mutant strain lacking both potassium efflux and uptake transporters (ena1-4delta nha1delta trk1delta trk2delta) enabled the monitoring of channel activity on two levels--the provision of the necessary amount of intracellular K+ in media with low potassium concentrations, and simultaneously, the channel"s contribution to cell potassium sensitivity in the presence of high external K+. Potassium 40-49 Trk1p Saccharomyces cerevisiae S288C 82-123 22329368-2 2012 Lost potassium is taken up by the Trk1 and Trk2 uptake systems. Potassium 5-14 Trk1p Saccharomyces cerevisiae S288C 34-38 22329368-5 2012 In trk1Delta trk2Delta mutants, the potassium efflux via potassium exporters Nha1 and Ena1 is diminished and can be restored either by the expression of TRK1 or deletion of TOK1. Potassium 36-45 Trk1p Saccharomyces cerevisiae S288C 3-7 22329368-5 2012 In trk1Delta trk2Delta mutants, the potassium efflux via potassium exporters Nha1 and Ena1 is diminished and can be restored either by the expression of TRK1 or deletion of TOK1. Potassium 36-45 Trk1p Saccharomyces cerevisiae S288C 153-157 22737060-6 2012 In contrast to the prevailing view, we show that regulation of the main potassium transport systems (Trk1,2 and Nha1) in the plasma membrane is not sufficient to achieve homeostasis. Potassium 72-81 Trk1p Saccharomyces cerevisiae S288C 101-105 22950024-0 2012 Adaptation to potassium starvation of wild-type and K(+)-transport mutant (trk1,2) of Saccharomyces cerevisiae: 2-dimensional gel electrophoresis-based proteomic approach. Potassium 14-23 Trk1p Saccharomyces cerevisiae S288C 75-81 20638488-1 2010 By using a 2-DE based workflow, the proteome of wild and potassium transport mutant trk1,2 under optimal growth potassium concentration (50mM) has been analyzed. Potassium 57-66 Trk1p Saccharomyces cerevisiae S288C 84-88 20412803-1 2010 The phosphatase calcineurin and the kinases Hal4/Hal5 regulate high-affinity potassium uptake in Saccharomyces cerevisiae through the Trk1 transporter. Potassium 77-86 Trk1p Saccharomyces cerevisiae S288C 134-138 16358319-7 2005 The use of a mutant strain lacking both potassium efflux and uptake transporters (ena1-4delta nha1delta trk1delta trk2delta) enabled the monitoring of channel activity on two levels--the provision of the necessary amount of intracellular K+ in media with low potassium concentrations, and simultaneously, the channel"s contribution to cell potassium sensitivity in the presence of high external K+. Potassium 259-268 Trk1p Saccharomyces cerevisiae S288C 82-123 16358319-7 2005 The use of a mutant strain lacking both potassium efflux and uptake transporters (ena1-4delta nha1delta trk1delta trk2delta) enabled the monitoring of channel activity on two levels--the provision of the necessary amount of intracellular K+ in media with low potassium concentrations, and simultaneously, the channel"s contribution to cell potassium sensitivity in the presence of high external K+. Potassium 259-268 Trk1p Saccharomyces cerevisiae S288C 82-123 12535075-0 2003 Characterization of potassium transport in wild-type and isogenic yeast strains carrying all combinations of trk1, trk2 and tok1 null mutations. Potassium 20-29 Trk1p Saccharomyces cerevisiae S288C 109-113 15581616-1 2004 The Ppz protein phosphatases have been recently shown to negatively regulate the major potassium transport system in the yeast Saccharomyces cerevisiae, encoded by the TRK1 and TRK2 genes. Potassium 87-96 Trk1p Saccharomyces cerevisiae S288C 168-172 15216418-1 2004 The TRK proteins-Trk1p and Trk2p- are the main agents responsible for "active" accumulation of potassium by the yeast Saccharomyces cerevisiae. Potassium 95-104 Trk1p Saccharomyces cerevisiae S288C 17-22 12535075-1 2003 Saccharomyces cerevisiae cells express three defined potassium-specific transport systems en-coded by TRK1, TRK2 and TOK1. Potassium 53-62 Trk1p Saccharomyces cerevisiae S288C 102-106 12535075-4 2003 As has been reported previously, Trk1p and Trk2p facilitate high-affinity potassium uptake and appear to be functionally redundant under a wide range of environmental conditions. Potassium 74-83 Trk1p Saccharomyces cerevisiae S288C 33-38 11967830-1 2002 Potassium uptake in Saccharomyces cerevisiae is mediated by at least two proteins, known as Trk1p and Trk2p. Potassium 0-9 Trk1p Saccharomyces cerevisiae S288C 92-97 11967830-7 2002 KlTrkp is a 1070 amino acid peptide that shows, overall, higher homology with Trk2p than with Trk1p, and its disruption gives rise to cells with deficient potassium transport and with an increased K(+) requirement for normal growth. Potassium 155-164 Trk1p Saccharomyces cerevisiae S288C 94-99 12101003-0 2002 Molecular analysis of the mechanism of potassium uptake through the TRK1 transporter of Saccharomyces cerevisiae. Potassium 39-48 Trk1p Saccharomyces cerevisiae S288C 68-72 9614085-0 1998 Ectopic potassium uptake in trk1 trk2 mutants of Saccharomyces cerevisiae correlates with a highly hyperpolarized membrane potential. Potassium 8-17 Trk1p Saccharomyces cerevisiae S288C 28-32 11779561-6 2002 Now, demonstration of multiple parallel effects produced by various agonists and antagonists on both NSC1 currents and growth (of trk1 Delta trk2 Delta strains), has identified this non-selective cation pathway as the primary low-affinity uptake route for potassium ions in yeast. Potassium 256-265 Trk1p Saccharomyces cerevisiae S288C 130-134 10207057-5 1999 These phenotypic effects of the mutations correlate with changes in cation uptake and are dependent on a functional Trk1-Trk2 potassium transport system. Potassium 126-135 Trk1p Saccharomyces cerevisiae S288C 116-120 10207057-6 1999 In addition, hal4 hal5 and trk1 trk2 mutants exhibit similar phenotypes: (i) they are deficient in potassium uptake; (ii) their growth is sensitive to a variety of toxic cations, including lithium, sodium, calcium, tetramethylammonium, hygromycin B, and low pH; and (iii) they exhibit increased uptake of methylammonium, an indicator of membrane potential. Potassium 99-108 Trk1p Saccharomyces cerevisiae S288C 27-31 11754478-6 2002 We propose that Nha1p regulates the potassium content of the cell and, as a consequence, can affect the activity of the main K(+) influx system (Trk1p). Potassium 36-45 Trk1p Saccharomyces cerevisiae S288C 145-150 10471809-1 1999 The Schizosaccharomyces pombe Tpr1 was isolated as suppressor of the Saccharomyces cerevisiae Delta trk1,2 potassium uptake deficient phenotype. Potassium 107-116 Trk1p Saccharomyces cerevisiae S288C 100-104 10207057-7 1999 These results suggest that the Hal4 and Hal5 protein kinases activate the Trk1-Trk2 potassium transporter, increasing the influx of potassium and decreasing the membrane potential. Potassium 84-93 Trk1p Saccharomyces cerevisiae S288C 74-78 9447989-1 1998 Deletion of TRK1 and TRK2 abolishes high-affinity K+ uptake in Saccharomyces cerevisiae, resulting in the inability to grow on typical synthetic growth medium unless it is supplemented with very high concentrations of potassium. Potassium 218-227 Trk1p Saccharomyces cerevisiae S288C 12-16 9139127-1 1996 Complementary DNAs involved in potassium transport in Schizosaccharomyces pombe were selected by complementation of defective K+ uptake in a trk1 trk2 mutant of Saccharomyces cerevisiae. Potassium 31-40 Trk1p Saccharomyces cerevisiae S288C 141-145 9153214-1 1997 Deletion of the potassium transporter genes TRK1 and TRK2 impairs potassium uptake in Saccharomyces cerevisiae, resulting in a greatly increased requirement for the ion and the inability to grow on low pH medium. Potassium 16-25 Trk1p Saccharomyces cerevisiae S288C 44-48 8534918-7 1995 gpIRK1 complements a trk1 delta trk2 delta strain, which is defective in potassium uptake. Potassium 73-82 Trk1p Saccharomyces cerevisiae S288C 21-25 34170815-3 2021 S. cerevisiae cells possess two transporters, Trk1 and Trk2, which ensure a high intracellular concentration of potassium, necessary for many physiological processes. Potassium 112-121 Trk1p Saccharomyces cerevisiae S288C 46-50 34170815-4 2021 Trk1 is the major system responsible for potassium acquisition in growing and dividing cells. Potassium 41-50 Trk1p Saccharomyces cerevisiae S288C 0-4 34170815-7 2021 Our results also demonstrate that Trk2 is much more important for the cell survival of glucose-induced cell death than Trk1, and that stationary cells deficient in active potassium uptake lose their ATP stocks more rapidly than cells with functional Trk systems. Potassium 171-180 Trk1p Saccharomyces cerevisiae S288C 119-123 31346773-3 2019 We show in this work that the activity of this transporter is regulated at the posttranslational level, and thus Trk1 contributes to potassium uptake under very different external cation concentrations. Potassium 133-142 Trk1p Saccharomyces cerevisiae S288C 113-117 7597651-6 1994 For example, since the wild-type KAT1 K+ channel reduces the potassium requirement of trk1 delta trk2 delta cells from approximately 50 mM to less than 50 microM, the function of mutant channels can be assessed over a 1,000-fold range in concentration of the permeant ion. Potassium 61-70 Trk1p Saccharomyces cerevisiae S288C 86-90 2199313-1 1990 Saccharomyces cerevisiae cells containing a deletion of TRK1, the gene encoding the high affinity potassium transporter, retain only low affinity uptake of this ion and consequently lose the ability to grow in media containing low levels (0.2 mM) of potassium. Potassium 98-107 Trk1p Saccharomyces cerevisiae S288C 56-60 2199313-2 1990 Using a trk1 delta strain, we selected spontaneous Trk+ pseudorevertants that regained the ability to grow on low concentrations of potassium. Potassium 132-141 Trk1p Saccharomyces cerevisiae S288C 8-12 2199313-4 1990 Dominant RPD2 mutations and recessive rpd1 and rpd3 mutations confer increased potassium uptake in trk1 delta cells. Potassium 79-88 Trk1p Saccharomyces cerevisiae S288C 99-103 35628688-3 2022 Trk1 has been shown to exist in low- or high-affinity modes, which reflect the availability of potassium in the environment. Potassium 95-104 Trk1p Saccharomyces cerevisiae S288C 0-4 3043197-0 1988 TRK1 encodes a plasma membrane protein required for high-affinity potassium transport in Saccharomyces cerevisiae. Potassium 66-75 Trk1p Saccharomyces cerevisiae S288C 0-4 3043197-2 1988 The gene that encodes this putative potassium transporter (TRK1) was cloned by its ability to relieve the potassium transport defect in trk1 cells. Potassium 36-45 Trk1p Saccharomyces cerevisiae S288C 59-63 3043197-2 1988 The gene that encodes this putative potassium transporter (TRK1) was cloned by its ability to relieve the potassium transport defect in trk1 cells. Potassium 36-45 Trk1p Saccharomyces cerevisiae S288C 136-140 3043197-6 1988 Haploid cells that contain a null allele of TRK1 (trk1 delta) rely on a low-affinity transporter for potassium uptake and, under certain conditions, exhibit energy-dependent loss of potassium, directly exposing the activity of a transporter responsible for the efflux of this ion. Potassium 101-110 Trk1p Saccharomyces cerevisiae S288C 44-48 3043197-6 1988 Haploid cells that contain a null allele of TRK1 (trk1 delta) rely on a low-affinity transporter for potassium uptake and, under certain conditions, exhibit energy-dependent loss of potassium, directly exposing the activity of a transporter responsible for the efflux of this ion. Potassium 101-110 Trk1p Saccharomyces cerevisiae S288C 50-54 3043197-6 1988 Haploid cells that contain a null allele of TRK1 (trk1 delta) rely on a low-affinity transporter for potassium uptake and, under certain conditions, exhibit energy-dependent loss of potassium, directly exposing the activity of a transporter responsible for the efflux of this ion. Potassium 182-191 Trk1p Saccharomyces cerevisiae S288C 44-48 3043197-6 1988 Haploid cells that contain a null allele of TRK1 (trk1 delta) rely on a low-affinity transporter for potassium uptake and, under certain conditions, exhibit energy-dependent loss of potassium, directly exposing the activity of a transporter responsible for the efflux of this ion. Potassium 182-191 Trk1p Saccharomyces cerevisiae S288C 50-54 31136755-0 2019 Erv14 cargo receptor participates in regulation of plasma-membrane potential, intracellular pH and potassium homeostasis via its interaction with K+-specific transporters Trk1 and Tok1. Potassium 99-108 Trk1p Saccharomyces cerevisiae S288C 171-175 31044010-0 2019 Evolutionary engineering in Saccharomyces cerevisiae reveals a TRK1-dependent potassium influx mechanism for propionic acid tolerance. Potassium 78-87 Trk1p Saccharomyces cerevisiae S288C 63-67 31044010-6 2019 Potassium supplementation growth assays showed that mutated TRK1 alleles and extracellular potassium supplementation not only conferred tolerance to PA stress but also to multiple organic acids. Potassium 0-9 Trk1p Saccharomyces cerevisiae S288C 60-64