PMID-sentid	Pub_year	Sent_text		comp_official_name	comp_offsetprotein_name	organism	prot_offset
30124645-5	2018	Release of ortho-nitrophenol following the hydrolytic cleavage of ONPG by lactase is measured by a change in absorbance at 420 nm, and the effect of the temperature on the enzymatic reaction is evaluated by carrying out the reaction on ice, at room temperature and at 37  C. More advanced analysis can be implemented using this protocol by assessing the enzyme activity under different conditions and using different reagents.	2-nitrophenol	11-28	lactase	Homo sapiens	74-81