PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 28072464-0 2017 Genistein and tyrphostin AG556 decrease ultra-rapidly activating delayed rectifier K+ current of human atria by inhibiting EGF receptor tyrosine kinase. Genistein 0-9 epidermal growth factor Homo sapiens 123-126 21486282-8 2011 The decrease of tyrosine phosphorylation of K(IR) 2.3 channels by genistein or AG556 was reversed by orthovanadate or EGF. Genistein 66-75 epidermal growth factor Homo sapiens 118-121 21639915-0 2011 Genistein inhibits proliferation of colon cancer cells by attenuating a negative effect of epidermal growth factor on tumor suppressor FOXO3 activity. Genistein 0-9 epidermal growth factor Homo sapiens 91-114 21639915-5 2011 METHODS: The effect of genistein on proliferation stimulated by EGF-mediated loss of FOXO3 was examined in human colonic cancer HT-29 cells. Genistein 23-32 epidermal growth factor Homo sapiens 64-67 21639915-6 2011 EGF-induced FOXO3 phosphorylation and translocation were assessed in the presence of genistein. Genistein 85-94 epidermal growth factor Homo sapiens 0-3 21639915-9 2011 RESULTS: Genistein inhibited EGF-induced proliferation, while favoring dephosphorylation and nuclear retention of FOXO3 (active state) in colon cancer cells. Genistein 9-18 epidermal growth factor Homo sapiens 29-32 21639915-10 2011 Upstream of FOXO3, genistein acts via the PI3K/Akt pathway to inhibit EGF-stimulated FOXO3 phosphorylation (i.e. favors active state). Genistein 19-28 epidermal growth factor Homo sapiens 70-73 19288529-0 2009 Genistein and curcumin suppress epidermal growth factor-induced MUC5AC mucin production and gene expression from human airway epithelial cells. Genistein 0-9 epidermal growth factor Homo sapiens 32-55 19288529-1 2009 This study investigated whether genistein and curcumin affect epidermal growth factor (EGF)-induced MUC5AC mucin production and gene expression from human airway epithelial cells. Genistein 32-41 epidermal growth factor Homo sapiens 62-85 19288529-1 2009 This study investigated whether genistein and curcumin affect epidermal growth factor (EGF)-induced MUC5AC mucin production and gene expression from human airway epithelial cells. Genistein 32-41 epidermal growth factor Homo sapiens 87-90 19288529-3 2009 The results were as follows: (1) genistein and curcumin inhibited the production of MUC5AC mucin protein induced by EGF, dose-dependently; (2) genistein and curcumin also inhibited the expression of MUC5AC mucin gene induced by EGF. Genistein 33-42 epidermal growth factor Homo sapiens 116-119 19288529-3 2009 The results were as follows: (1) genistein and curcumin inhibited the production of MUC5AC mucin protein induced by EGF, dose-dependently; (2) genistein and curcumin also inhibited the expression of MUC5AC mucin gene induced by EGF. Genistein 33-42 epidermal growth factor Homo sapiens 228-231 19288529-3 2009 The results were as follows: (1) genistein and curcumin inhibited the production of MUC5AC mucin protein induced by EGF, dose-dependently; (2) genistein and curcumin also inhibited the expression of MUC5AC mucin gene induced by EGF. Genistein 143-152 epidermal growth factor Homo sapiens 116-119 19288529-3 2009 The results were as follows: (1) genistein and curcumin inhibited the production of MUC5AC mucin protein induced by EGF, dose-dependently; (2) genistein and curcumin also inhibited the expression of MUC5AC mucin gene induced by EGF. Genistein 143-152 epidermal growth factor Homo sapiens 228-231 19288529-4 2009 This result suggests that genistein and curcumin can regulate mucin gene expression and production of mucin protein induced by EGF, by directly acting on airway epithelial cells. Genistein 26-35 epidermal growth factor Homo sapiens 127-130 19272193-0 2009 Genistein-mediated inhibition of glycosaminoglycan synthesis, which corrects storage in cells of patients suffering from mucopolysaccharidoses, acts by influencing an epidermal growth factor-dependent pathway. Genistein 0-9 epidermal growth factor Homo sapiens 167-190 19272193-6 2009 METHODS: To learn on details of the molecular mechanism of genistein-mediated inhibition of GAG synthesis, efficiency of this process was studied by measuring of incorporation of labeled sulfate, storage of GAGs in lysosomes was estimated by using electron microscopic techniques, and efficiency of phosphorylation of epidermal growth factor (EGF) receptor was determined by using an ELISA-based assay with fluorogenic substrates. Genistein 59-68 epidermal growth factor Homo sapiens 318-341 19272193-6 2009 METHODS: To learn on details of the molecular mechanism of genistein-mediated inhibition of GAG synthesis, efficiency of this process was studied by measuring of incorporation of labeled sulfate, storage of GAGs in lysosomes was estimated by using electron microscopic techniques, and efficiency of phosphorylation of epidermal growth factor (EGF) receptor was determined by using an ELISA-based assay with fluorogenic substrates. Genistein 59-68 epidermal growth factor Homo sapiens 343-346 19272193-7 2009 RESULTS: Effects of genistein on inhibition of GAG synthesis and accumulation in fibroblasts from patients suffering from various MPS types were abolished in the presence of an excess of EGF, and were partially reversed by an increased concentration of genistein. Genistein 20-29 epidermal growth factor Homo sapiens 187-190 19272193-9 2009 Moreover, EGF-mediated stimulation of phsophorylation of the EGF receptor was impaired in the presence of genistein in both wild-type and MPS fibroblasts. Genistein 106-115 epidermal growth factor Homo sapiens 10-13 19272193-9 2009 Moreover, EGF-mediated stimulation of phsophorylation of the EGF receptor was impaired in the presence of genistein in both wild-type and MPS fibroblasts. Genistein 106-115 epidermal growth factor Homo sapiens 61-64 19272193-10 2009 CONCLUSION: The results presented in this report indicate that the mechanism of genistein-mediated inhibition of GAG synthesis operates through epidermal growth factor (EGF)-dependent pathway. Genistein 80-89 epidermal growth factor Homo sapiens 144-167 19272193-10 2009 CONCLUSION: The results presented in this report indicate that the mechanism of genistein-mediated inhibition of GAG synthesis operates through epidermal growth factor (EGF)-dependent pathway. Genistein 80-89 epidermal growth factor Homo sapiens 169-172 16837648-9 2007 Genistein, a nonspecific phosphorylation inhibitor, increased the inward potassium current in IC BUC and blocked the effect of EGF on normal BUC. Genistein 0-9 epidermal growth factor Homo sapiens 127-130 16598420-8 2006 Genistein also induced a decrease in EGF-stimulated invasion thereby implicating an involvement of EGF-mediated signaling in invasion. Genistein 0-9 epidermal growth factor Homo sapiens 37-40 16598420-8 2006 Genistein also induced a decrease in EGF-stimulated invasion thereby implicating an involvement of EGF-mediated signaling in invasion. Genistein 0-9 epidermal growth factor Homo sapiens 99-102 15491342-9 2004 Exposure to genistein and curcumin inhibited EGF-stimulated urokinase production, although only genistein showed a statistically significant inhibitory response. Genistein 12-21 epidermal growth factor Homo sapiens 45-48 15491342-13 2004 Finally, EGF stimulated the phosphorylation of its receptor and tyrphostin (AG1478), curcumin and genistein were able to inhibit this stimulatory effect. Genistein 98-107 epidermal growth factor Homo sapiens 9-12 15491342-15 2004 Our data also shows that EGF-stimulated uPA production involves the activation of the extracellular signal-regulated kinases 1/2 and JNK signaling pathways and might be modulated by the natural phytoestrogens curcumin and genistein. Genistein 222-231 epidermal growth factor Homo sapiens 25-28 12532446-7 2003 RESULTS: EGF (10(-9) M) stimulated the proliferation of heptoma cells significantly ((3)H-TdR incorporation was 1 880+/-281 cpm/well, P<0.05), and this effect was significantly inhibited by tyrosine kinase inhibitor genistein ((3)H-TdR incorporation was 808+/-209 cpm/well, P<0.001). Genistein 219-228 epidermal growth factor Homo sapiens 9-12 14610335-3 2003 EGF-induced inhibition of Pi uptake was blocked by AG1478 (an EGF receptor antagonist), genistein or herbimycin A (tyrosine kinase inhibitors) and also blocked by mepacrine (a phospholipase A(2) (PLA(2)) inhibitor) and AACOCF(3 )(a cPLA(2) inhibitor). Genistein 88-97 epidermal growth factor Homo sapiens 0-3 14610335-4 2003 EGF increased [(3)H]-arachidonic acid (AA) release, which was also blocked by AG1478, genistein or herbimycin. Genistein 86-95 epidermal growth factor Homo sapiens 0-3 12208089-6 2002 Genistein, one of the tyrosine-kinase inhibitors, abolished the augmentation effect of EGF on the ACh-induced current. Genistein 0-9 epidermal growth factor Homo sapiens 87-90 12141529-7 2002 Genistein, tryphostin AG1478, and wortmannin partially suppressed the increase in production induced by EGF (P<0.01, P<0.01, P<0.01), respectively. Genistein 0-9 epidermal growth factor Homo sapiens 104-107 11356157-2 2001 Epidermal growth factor (EGF)-stimulated proliferation of human mammary epithelial cells (that do not express the oestrogen receptor) was significantly suppressed at genistein concentrations (5-10 microM) that are attainable physiologically. Genistein 166-175 epidermal growth factor Homo sapiens 0-23 11356157-2 2001 Epidermal growth factor (EGF)-stimulated proliferation of human mammary epithelial cells (that do not express the oestrogen receptor) was significantly suppressed at genistein concentrations (5-10 microM) that are attainable physiologically. Genistein 166-175 epidermal growth factor Homo sapiens 25-28 11356157-4 2001 Analysis of the conditioned medium of human mammary epithelial cells exposed to genistein plus EGF showed increased levels of TGFbeta relative to those in the medium of cells exposed to EGF or genistein alone. Genistein 80-89 epidermal growth factor Homo sapiens 186-189 11356157-4 2001 Analysis of the conditioned medium of human mammary epithelial cells exposed to genistein plus EGF showed increased levels of TGFbeta relative to those in the medium of cells exposed to EGF or genistein alone. Genistein 193-202 epidermal growth factor Homo sapiens 95-98 11229455-13 2001 This EGF-stimulated phosphorylation was completely abrogated by genistein and herceptin. Genistein 64-73 epidermal growth factor Homo sapiens 5-8 11182513-5 2001 Complete suppression of cumulus expansion stimulated by LS and EGF was observed in the presence of 10 micromol/l genistein (tyrosine kinase inhibitor), in the presence of 10mmol/l LiCl (the inhibitor of inositol 1,4,5-trisphosphate metabolism), and 100 micromol/l gallopamil, verapamil and norverapamil (calcium channel blockers). Genistein 113-122 epidermal growth factor Homo sapiens 63-66 11114114-5 2000 Thus, genistein, a tyrosine kinase inhibitor, was capable of reducing by half the tyrosine phosphorylation caused by the binding of EGF to A431 cells, whereas 6-carboxymethyl genistein did not inhibit protein tyrosine phosphorylation. Genistein 6-15 epidermal growth factor Homo sapiens 132-135 10037455-7 1999 The EGF-mediated protection was prevented by treatment of cell monolayers with genistein (10 microM), a tyrosine kinase inhibitor. Genistein 79-88 epidermal growth factor Homo sapiens 4-7 9701629-8 1998 In addition, whereas the mitogenic activity of 10 ng/ml epidermal growth factor (EGF) on human mandible-derived bone cells was completely abolished by 5-30 microM tyrphostin A51, genistein at 5-30 microM enhanced the EGF-induced bone cell proliferation in an additive manner. Genistein 179-188 epidermal growth factor Homo sapiens 56-79 9701629-8 1998 In addition, whereas the mitogenic activity of 10 ng/ml epidermal growth factor (EGF) on human mandible-derived bone cells was completely abolished by 5-30 microM tyrphostin A51, genistein at 5-30 microM enhanced the EGF-induced bone cell proliferation in an additive manner. Genistein 179-188 epidermal growth factor Homo sapiens 81-84 9849427-4 1998 Of the six agents, only genistein decreased EGF-induced, c-fos transcription (by 63% compared to control at 100 mumol/l). Genistein 24-33 epidermal growth factor Homo sapiens 44-47 9849427-8 1998 None of the agents inhibited protein kinase C and only genistein inhibited EGF receptor-linked protein tyrosine kinase obtained from MDA-MB-468 cells, with an IC50 of 60 mumol/l. Genistein 55-64 epidermal growth factor Homo sapiens 75-78 9849427-11 1998 Inhibition by genistein of EGF-induced c-fos mRNA transcription is probably related to its interruption of EGF receptor-linked protein tyrosine kinase, whereas genistein-induced growth arrest is not. Genistein 14-23 epidermal growth factor Homo sapiens 27-30 9849427-11 1998 Inhibition by genistein of EGF-induced c-fos mRNA transcription is probably related to its interruption of EGF receptor-linked protein tyrosine kinase, whereas genistein-induced growth arrest is not. Genistein 14-23 epidermal growth factor Homo sapiens 107-110 9612280-4 1998 However, TPA-induced alkalosis is not blocked by tyrosine kinase inhibitors; and 3) the stimulatory effect of EGF on the Na(+)-H+ exchanger acts via stimulation of tyrosine kinase-receptor activity because it is inhibited by tyrosine kinase inhibitors (genistein, lavendustin A, and herbimycin A). Genistein 253-262 epidermal growth factor Homo sapiens 110-113 9588219-4 1998 Genistein abolished the ability of EGF to induce EGFR phosphorylation, to activate MAP kinase and to increase cell proliferation. Genistein 0-9 epidermal growth factor Homo sapiens 35-38 9588219-5 1998 Genistein alone stimulated CLE competence, but apparently by a different mechanism than EGF since genistein prevented EGF-stimulated CLE competence. Genistein 0-9 epidermal growth factor Homo sapiens 118-121 9588219-5 1998 Genistein alone stimulated CLE competence, but apparently by a different mechanism than EGF since genistein prevented EGF-stimulated CLE competence. Genistein 98-107 epidermal growth factor Homo sapiens 118-121 9575872-9 1998 Furthermore, exposure to genistein also decreases SP-A expression and blocks the effects of EGF in human fetal lung tissue without inhibiting EGF-receptor tyrosine phosphorylation. Genistein 25-34 epidermal growth factor Homo sapiens 92-95 9563884-2 1998 Recombinant human EGF was conjugated to the soybean-derived PTK inhibitor genistein (Gen) to construct an EGF-R-directed cytotoxic agent with PTK inhibitory activity. Genistein 74-83 epidermal growth factor Homo sapiens 18-21 9563884-2 1998 Recombinant human EGF was conjugated to the soybean-derived PTK inhibitor genistein (Gen) to construct an EGF-R-directed cytotoxic agent with PTK inhibitory activity. Genistein 74-83 epidermal growth factor Homo sapiens 106-109 9563884-2 1998 Recombinant human EGF was conjugated to the soybean-derived PTK inhibitor genistein (Gen) to construct an EGF-R-directed cytotoxic agent with PTK inhibitory activity. Genistein 85-88 epidermal growth factor Homo sapiens 18-21 9563884-2 1998 Recombinant human EGF was conjugated to the soybean-derived PTK inhibitor genistein (Gen) to construct an EGF-R-directed cytotoxic agent with PTK inhibitory activity. Genistein 85-88 epidermal growth factor Homo sapiens 106-109 9851317-5 1998 In contrast, genistein (10 mg x ml(-1)), an inhibitor of tyrosine kinase, prevented EGF-dependent stimulation of aminopeptidase N and 5"-nucleotidase, suggesting that protein phosphorylation was involved in the signaling mechanism. Genistein 13-22 epidermal growth factor Homo sapiens 84-87 9770719-4 1998 Chrysin at 1.0-10 microM and 10 microM luteolin or apigenin inhibited E2-induced DNA synthesis, as did all compounds at > 10 microM, 2) tamoxifen enhanced genistein-induced DNA synthesis but inhibited DNA synthesis induced by all other compounds, and 3) genistein enhanced insulin- and epidermal growth factor-induced DNA synthesis at 0.1-1.0 and 0.1-10 microM, respectively. Genistein 158-167 epidermal growth factor Homo sapiens 276-312 9406144-2 1997 Most of these studies have utilized highly selective inhibitors of PTKs, genistein and tyrphostin and have shown that these inhibitors attenuated smooth muscle contraction induced by growth factors-epidermal growth factor (EGF) and platelet derived growth factor (PDGF) and several vasoactive peptides. Genistein 73-82 epidermal growth factor Homo sapiens 190-221 9366260-6 1997 Moreover, the confined radiation-induced phosphorylation of tyrosine residues is inhibited by genistein, indicating that this phosphorylation of EGF receptor is due to protein tyrosine kinase activation. Genistein 94-103 epidermal growth factor Homo sapiens 145-148 9269996-5 1997 In 647V spheroids, EGF protection against radiation-induced apoptosis was negated by genistein and tyrphostin AG1478, suggesting that blockade of the EGF signal transduction in patients with bladder cancer may improve the radiotherapy efficacy. Genistein 85-94 epidermal growth factor Homo sapiens 19-22 9084414-8 1997 Genistein, a tyrosine kinase inhibitor, was able to block the stimulatory effect of ATA and EGF. Genistein 0-9 epidermal growth factor Homo sapiens 92-95 8843722-8 1996 In contrast, genistein inhibited the rise in DL-Ins(3,4,5,6)P4 and partially reversed EGF"s inhibitory effect on Cl- secretion. Genistein 13-22 epidermal growth factor Homo sapiens 86-89 8761379-7 1996 The tyrosine kinase inhibitors genistein and tyrphostin-47 inhibited EGF-induced protein tyrosine phosphorylation dose-dependently in the 21HKE cells, and abolished the growth stimulatory effect of docosahexaenoic acid (DHA). Genistein 31-40 epidermal growth factor Homo sapiens 69-72 8702388-0 1996 Genistein, a tyrosine kinase inhibitor, reduces EGF-induced EGF receptor internalization and degradation in human hepatoma HepG2 cells. Genistein 0-9 epidermal growth factor Homo sapiens 48-51 8702388-1 1996 In this work, using the ECL Western blotting assay system, it was found that genistein, a specific tyrosine kinase inhibitor, was able to inhibit EGF-induced EGF receptor degradation and tyrosine phosphorylation in human hepatoma HepG2 cells. Genistein 77-86 epidermal growth factor Homo sapiens 146-149 8702388-3 1996 With treatment of HepG2 cells with genistein at 37 degrees C for 30 min, the amount of internalized EGF, which was measured by the detection of the sorting of 125I-EGF in the cells, was remarkably decreased. Genistein 35-44 epidermal growth factor Homo sapiens 100-103 8702388-3 1996 With treatment of HepG2 cells with genistein at 37 degrees C for 30 min, the amount of internalized EGF, which was measured by the detection of the sorting of 125I-EGF in the cells, was remarkably decreased. Genistein 35-44 epidermal growth factor Homo sapiens 164-167 8702388-5 1996 After preincubation of HepG2 cells with and without genistein for 120 min at 37 degrees C, the ratio between degraded and released EGF was 16 and 24, respectively. Genistein 52-61 epidermal growth factor Homo sapiens 131-134 7858744-8 1995 We conclude that TGF-alpha stimulates invasion and growth of follicular thyroid cancer by binding to the EGF receptors, that EGF- and TGF-alpha-mediated effects can be blocked by antagonism to the EGF receptor and to tyrosine kinase, and that genistein not only neutralized EGF and TGF-alpha effects but also inhibited invasion and growth of unstimulated FTC133. Genistein 243-252 epidermal growth factor Homo sapiens 125-128 7858744-8 1995 We conclude that TGF-alpha stimulates invasion and growth of follicular thyroid cancer by binding to the EGF receptors, that EGF- and TGF-alpha-mediated effects can be blocked by antagonism to the EGF receptor and to tyrosine kinase, and that genistein not only neutralized EGF and TGF-alpha effects but also inhibited invasion and growth of unstimulated FTC133. Genistein 243-252 epidermal growth factor Homo sapiens 125-128 7858744-8 1995 We conclude that TGF-alpha stimulates invasion and growth of follicular thyroid cancer by binding to the EGF receptors, that EGF- and TGF-alpha-mediated effects can be blocked by antagonism to the EGF receptor and to tyrosine kinase, and that genistein not only neutralized EGF and TGF-alpha effects but also inhibited invasion and growth of unstimulated FTC133. Genistein 243-252 epidermal growth factor Homo sapiens 125-128 7832811-7 1995 The tyrosine kinase inhibitor, genistein, completely inhibited EGF-stimulated polyamine uptake, indicating that tyrosine phosphorylation plays a role in EGF-stimulated polyamine uptake in Caco-2 cells. Genistein 31-40 epidermal growth factor Homo sapiens 63-66 7832811-7 1995 The tyrosine kinase inhibitor, genistein, completely inhibited EGF-stimulated polyamine uptake, indicating that tyrosine phosphorylation plays a role in EGF-stimulated polyamine uptake in Caco-2 cells. Genistein 31-40 epidermal growth factor Homo sapiens 153-156 7927239-6 1994 The epidermal growth factor-induced intracellular pH increase was inhibited by pretreatment of hepatocytes with genistein (100 mumol/L), thapsigargin (3 mumol/L) or calmodulin inhibitor W-7 (25 mumol/L), but not with protein kinase C inhibitor H-7 (50 mumol/L) or with cyclic AMP-dependent kinase inhibitor H-8 (60 mumol/L). Genistein 112-121 epidermal growth factor Homo sapiens 4-27 8062159-0 1994 Effects of genistein, tyrphostin, and pertussis toxin on EGF-induced mitogenesis in primary culture and clonal osteoblastic cells. Genistein 11-20 epidermal growth factor Homo sapiens 57-60 8062159-6 1994 Genistein was able to inhibit the effects of EGF in the G292 cells. Genistein 0-9 epidermal growth factor Homo sapiens 45-48 8062159-7 1994 In the primary culture cells, genistein with EGF appeared to enhance proliferation compared with EGF alone or genistein alone. Genistein 30-39 epidermal growth factor Homo sapiens 45-48 8297123-5 1993 EGF mediated growth stimulation was dose-dependently reversed by coincubation with genistein. Genistein 83-92 epidermal growth factor Homo sapiens 0-3 8224278-8 1993 The stimulation by EGF was attenuated by the treatment with genistein, 12-O-tetradecanoylphorbol-13-acetate, or thapsigargin. Genistein 60-69 epidermal growth factor Homo sapiens 19-22 1353641-9 1992 Genistein (100 mumol/L) inhibited basal and EGF-stimulated 3H-thymidine uptake. Genistein 0-9 epidermal growth factor Homo sapiens 44-47 2173488-5 1990 The tyrosine kinase activity of the EGF receptor was essential for the induction of ectoCa2(+)-ATPase, since enzyme induction was abolished by a tyrosine kinase inhibitor, genistein. Genistein 172-181 epidermal growth factor Homo sapiens 36-39