PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 31438633-3 2019 Genistein down-regulated the levels of cyclin A and cyclin B1, but up-regulated the levels of p21WAF1/CIP1, cyclin-dependent kinase (Cdk) inhibitor, that was complexed with Cdc2 and Cdk2. Genistein 0-9 cyclin dependent kinase inhibitor 1A Homo sapiens 102-106 26003441-7 2015 In addition, genistein caused cell cycle arrest in the G2/M phase, which was accompanied by activation of CDKN1A, CDKN1C, CDKN2A, CDKN2B, CDKN2C, and GADD45A genes, as well as down-regulation of several mRNAs specific for this stage, demonstrated by transcriptomic assessments. Genistein 13-22 cyclin dependent kinase inhibitor 1A Homo sapiens 106-112 23686257-11 2013 In addition, genistein caused cell cycle arrest in the G2/M phase, which was accompanied by activation of ATM/p53, p21waf1/cip1 and GADD45alpha as well as downregulation of cdc2 and cdc25A demonstrated by q-PCR and immunoblotting assay. Genistein 13-22 cyclin dependent kinase inhibitor 1A Homo sapiens 115-127 25310747-5 2014 Genistein-induced ROS partially induced rapid transition into the G2/M phase by upregulation of p21wap1/cip1 and apoptotic cell death. Genistein 0-9 cyclin dependent kinase inhibitor 1A Homo sapiens 104-108 14757171-4 2004 Genistein also enhanced the expression of the cell cycle inhibitor, p21waf1/cip1, which interacts with Cdc2. Genistein 0-9 cyclin dependent kinase inhibitor 1A Homo sapiens 68-80 21843532-9 2011 This suggests the inhibitory effect of genistein on the induction of extra centrosomes occurs through the inactivation of CDK2/Cyclin-A/E via p21 up-regulation. Genistein 39-48 cyclin dependent kinase inhibitor 1A Homo sapiens 142-145 21843532-10 2011 This hypothesis is supported by the observation that p21 knockdown with siRNA reduced the activity of CDK2/Cyclin-A/E and restored the enhanced effect of a combined treatment with genistein and IR. Genistein 180-189 cyclin dependent kinase inhibitor 1A Homo sapiens 53-56 19263441-6 2009 By contrast, genistein only up-regulates cyclin-dependent kinase inhibitor 1A. Genistein 13-22 cyclin dependent kinase inhibitor 1A Homo sapiens 41-77 15179686-11 2004 Cell-cycle related protein analysis disclosed biphasic changes in cyclin B1, less markedly increased cdc-2 and stably elevated p21(cip1) levels with increasing genistein concentrations. Genistein 160-169 cyclin dependent kinase inhibitor 1A Homo sapiens 131-135 21843532-0 2011 Genistein, isoflavonoids in soybeans, prevents the formation of excess radiation-induced centrosomes via p21 up-regulation. Genistein 0-9 cyclin dependent kinase inhibitor 1A Homo sapiens 105-108 19038232-5 2009 Genistein stimulates p21(waf1/cip1) and cyclin B1 expression, phosphorylation/activation of ATM and Chk2 kinases, and Tyr15-phosphorylation/inactivation of Cdc2 (Cdk1) kinase, and these effects are attenuated by MEK/ERK inhibitors, while LY294002 also attenuates ERK and ATM phosphorylation. Genistein 0-9 cyclin dependent kinase inhibitor 1A Homo sapiens 30-34 18413741-0 2008 Genistein induces the p21WAF1/CIP1 and p16INK4a tumor suppressor genes in prostate cancer cells by epigenetic mechanisms involving active chromatin modification. Genistein 0-9 cyclin dependent kinase inhibitor 1A Homo sapiens 30-34 18413741-4 2008 Genistein induced the expression of tumor suppressor genes p21 (WAF1/CIP1/KIP1) and p16 (INK4a) with a concomitant decrease in cyclins. Genistein 0-9 cyclin dependent kinase inhibitor 1A Homo sapiens 59-62 18413741-4 2008 Genistein induced the expression of tumor suppressor genes p21 (WAF1/CIP1/KIP1) and p16 (INK4a) with a concomitant decrease in cyclins. Genistein 0-9 cyclin dependent kinase inhibitor 1A Homo sapiens 64-68 18413741-4 2008 Genistein induced the expression of tumor suppressor genes p21 (WAF1/CIP1/KIP1) and p16 (INK4a) with a concomitant decrease in cyclins. Genistein 0-9 cyclin dependent kinase inhibitor 1A Homo sapiens 69-73 18324703-2 2008 Using analysis of proteins related to cell cycle and apoptotic pathways, we confirmed an elevated level of p53 accompanying p21 Waf1/Cip1 protein in genistein-treated or genistin-treated A549 and WI-38 cells, but not in HeLa cells. Genistein 149-158 cyclin dependent kinase inhibitor 1A Homo sapiens 133-137 18357397-9 2008 There was an increase in the p53 and p21 levels in response to genistein. Genistein 63-72 cyclin dependent kinase inhibitor 1A Homo sapiens 37-40 16640785-7 2006 Cell cycle analysis demonstrated a significant dose- and time-dependent G2/M arrest induced by genistein and radiation that correlated with increased p21WAF1/Cip1 and decreased cyclin B1 expression. Genistein 95-104 cyclin dependent kinase inhibitor 1A Homo sapiens 158-162 16640785-11 2006 CONCLUSION: A mechanism of increased cell death by genistein and radiation is proposed to occur via inhibition of NF-kappaB, leading to altered expression of regulatory cell cycle proteins such as cyclin B and/or p21WAF1/Cip1, thus promoting G2/M arrest and increased radiosensitivity. Genistein 51-60 cyclin dependent kinase inhibitor 1A Homo sapiens 221-225 14757171-7 2004 Caffeine, an ATM kinase inhibitor, inhibited these effects of genistein on Chk2, p53, and p21waf1/cip1. Genistein 62-71 cyclin dependent kinase inhibitor 1A Homo sapiens 90-102 14757171-8 2004 These findings suggest that the effect of genistein on G2/M arrest in HepG2 cells is partly due to ATM-dependent Chk2 activation, an increase in Cdc2 phosphorylation/inactivation as a result of induction of Wee1 expression, and a decrease in Cdc2 activity as a result of induction of p21waf1/cip1 expression. Genistein 42-51 cyclin dependent kinase inhibitor 1A Homo sapiens 284-296 12767922-5 2003 Wortmannin, an ATM- and DNA-dependent protein kinase inhibitor, partially inhibited p21(WAF1/Cip1) expression induced by genistein and etoposide, whereas UCN-01, a Chk1 inhibitor, partially blocked etoposide, but not genistein-induced p21(WAF1/Cip1) expression. Genistein 121-130 cyclin dependent kinase inhibitor 1A Homo sapiens 239-243 12767922-0 2003 P21 response to DNA damage induced by genistein and etoposide in human lung cancer cells. Genistein 38-47 cyclin dependent kinase inhibitor 1A Homo sapiens 0-3 12767922-2 2003 Here we show that topoisomerase II inhibitors, genistein and etoposide, induce p21(WAF1/Cip1) expression mainly in a p53-dependent manner in human lung cancer cell line A549. Genistein 47-56 cyclin dependent kinase inhibitor 1A Homo sapiens 79-82 12767922-2 2003 Here we show that topoisomerase II inhibitors, genistein and etoposide, induce p21(WAF1/Cip1) expression mainly in a p53-dependent manner in human lung cancer cell line A549. Genistein 47-56 cyclin dependent kinase inhibitor 1A Homo sapiens 83-87 12767922-2 2003 Here we show that topoisomerase II inhibitors, genistein and etoposide, induce p21(WAF1/Cip1) expression mainly in a p53-dependent manner in human lung cancer cell line A549. Genistein 47-56 cyclin dependent kinase inhibitor 1A Homo sapiens 88-92 12767922-4 2003 Caffeine, an ataxia telangiectasia-mutated (ATM), and ATM- and Rad3-related kinase (ATR) inhibitor, abrogated genistein-induced p21(WAF1/Cip1) and largely blocked etoposide-induced p21(WAF1/Cip1) expression. Genistein 110-119 cyclin dependent kinase inhibitor 1A Homo sapiens 128-131 12767922-4 2003 Caffeine, an ataxia telangiectasia-mutated (ATM), and ATM- and Rad3-related kinase (ATR) inhibitor, abrogated genistein-induced p21(WAF1/Cip1) and largely blocked etoposide-induced p21(WAF1/Cip1) expression. Genistein 110-119 cyclin dependent kinase inhibitor 1A Homo sapiens 132-136 12767922-4 2003 Caffeine, an ataxia telangiectasia-mutated (ATM), and ATM- and Rad3-related kinase (ATR) inhibitor, abrogated genistein-induced p21(WAF1/Cip1) and largely blocked etoposide-induced p21(WAF1/Cip1) expression. Genistein 110-119 cyclin dependent kinase inhibitor 1A Homo sapiens 137-141 12767922-4 2003 Caffeine, an ataxia telangiectasia-mutated (ATM), and ATM- and Rad3-related kinase (ATR) inhibitor, abrogated genistein-induced p21(WAF1/Cip1) and largely blocked etoposide-induced p21(WAF1/Cip1) expression. Genistein 110-119 cyclin dependent kinase inhibitor 1A Homo sapiens 181-184 12767922-4 2003 Caffeine, an ataxia telangiectasia-mutated (ATM), and ATM- and Rad3-related kinase (ATR) inhibitor, abrogated genistein-induced p21(WAF1/Cip1) and largely blocked etoposide-induced p21(WAF1/Cip1) expression. Genistein 110-119 cyclin dependent kinase inhibitor 1A Homo sapiens 185-189 12767922-4 2003 Caffeine, an ataxia telangiectasia-mutated (ATM), and ATM- and Rad3-related kinase (ATR) inhibitor, abrogated genistein-induced p21(WAF1/Cip1) and largely blocked etoposide-induced p21(WAF1/Cip1) expression. Genistein 110-119 cyclin dependent kinase inhibitor 1A Homo sapiens 190-194 12767922-5 2003 Wortmannin, an ATM- and DNA-dependent protein kinase inhibitor, partially inhibited p21(WAF1/Cip1) expression induced by genistein and etoposide, whereas UCN-01, a Chk1 inhibitor, partially blocked etoposide, but not genistein-induced p21(WAF1/Cip1) expression. Genistein 121-130 cyclin dependent kinase inhibitor 1A Homo sapiens 84-87 12767922-5 2003 Wortmannin, an ATM- and DNA-dependent protein kinase inhibitor, partially inhibited p21(WAF1/Cip1) expression induced by genistein and etoposide, whereas UCN-01, a Chk1 inhibitor, partially blocked etoposide, but not genistein-induced p21(WAF1/Cip1) expression. Genistein 121-130 cyclin dependent kinase inhibitor 1A Homo sapiens 88-92 12767922-5 2003 Wortmannin, an ATM- and DNA-dependent protein kinase inhibitor, partially inhibited p21(WAF1/Cip1) expression induced by genistein and etoposide, whereas UCN-01, a Chk1 inhibitor, partially blocked etoposide, but not genistein-induced p21(WAF1/Cip1) expression. Genistein 121-130 cyclin dependent kinase inhibitor 1A Homo sapiens 93-97 12767922-5 2003 Wortmannin, an ATM- and DNA-dependent protein kinase inhibitor, partially inhibited p21(WAF1/Cip1) expression induced by genistein and etoposide, whereas UCN-01, a Chk1 inhibitor, partially blocked etoposide, but not genistein-induced p21(WAF1/Cip1) expression. Genistein 121-130 cyclin dependent kinase inhibitor 1A Homo sapiens 235-238 12767922-5 2003 Wortmannin, an ATM- and DNA-dependent protein kinase inhibitor, partially inhibited p21(WAF1/Cip1) expression induced by genistein and etoposide, whereas UCN-01, a Chk1 inhibitor, partially blocked etoposide, but not genistein-induced p21(WAF1/Cip1) expression. Genistein 121-130 cyclin dependent kinase inhibitor 1A Homo sapiens 244-248 12767922-6 2003 These data suggest that both genistein and etoposide induce p21(WAF1/Cip1) expression in a p53-dependent manner. Genistein 29-38 cyclin dependent kinase inhibitor 1A Homo sapiens 60-63 12767922-6 2003 These data suggest that both genistein and etoposide induce p21(WAF1/Cip1) expression in a p53-dependent manner. Genistein 29-38 cyclin dependent kinase inhibitor 1A Homo sapiens 64-68 12767922-6 2003 These data suggest that both genistein and etoposide induce p21(WAF1/Cip1) expression in a p53-dependent manner. Genistein 29-38 cyclin dependent kinase inhibitor 1A Homo sapiens 69-73 12767922-7 2003 Genistein appears to stimulate p21(WAF1/Cip1) expression through p53 via ATM, whereas etoposide may activate both ATM and ATR pathways. Genistein 0-9 cyclin dependent kinase inhibitor 1A Homo sapiens 31-34 12767922-7 2003 Genistein appears to stimulate p21(WAF1/Cip1) expression through p53 via ATM, whereas etoposide may activate both ATM and ATR pathways. Genistein 0-9 cyclin dependent kinase inhibitor 1A Homo sapiens 35-39 12767922-7 2003 Genistein appears to stimulate p21(WAF1/Cip1) expression through p53 via ATM, whereas etoposide may activate both ATM and ATR pathways. Genistein 0-9 cyclin dependent kinase inhibitor 1A Homo sapiens 40-44 12514295-2 2003 We recently reported that genistein treatment of the immortalized but nonmalignant human mammary epithelial cell line MCF-10F resulted in growth arrest of MCF-10F cells in the G2 phase of the cell cycle, a large induction of the Tyr15 phosphorylation of Cdc2 (along with decreased activity of Cdc2), increased expression of p21(waf/cip1) and decreased expression of the cell cycle phosphatase Cdc25C. Genistein 26-35 cyclin dependent kinase inhibitor 1A Homo sapiens 324-327 12514295-2 2003 We recently reported that genistein treatment of the immortalized but nonmalignant human mammary epithelial cell line MCF-10F resulted in growth arrest of MCF-10F cells in the G2 phase of the cell cycle, a large induction of the Tyr15 phosphorylation of Cdc2 (along with decreased activity of Cdc2), increased expression of p21(waf/cip1) and decreased expression of the cell cycle phosphatase Cdc25C. Genistein 26-35 cyclin dependent kinase inhibitor 1A Homo sapiens 332-336 11751518-5 2001 Oncol., 16: 333-338, 2000) that genistein induces cell cycle arrest and apoptosis by up-regulating p21(WAF1) and Bax, and down-regulating cyclin B1 and Bcl-2 in a head and neck cancer cell line. Genistein 32-41 cyclin dependent kinase inhibitor 1A Homo sapiens 99-102 11790449-3 2002 In addition, genistein induced the cyclin-dependent kinase inhibitor p21, which does not depend on p53 activation. Genistein 13-22 cyclin dependent kinase inhibitor 1A Homo sapiens 69-72 11751518-5 2001 Oncol., 16: 333-338, 2000) that genistein induces cell cycle arrest and apoptosis by up-regulating p21(WAF1) and Bax, and down-regulating cyclin B1 and Bcl-2 in a head and neck cancer cell line. Genistein 32-41 cyclin dependent kinase inhibitor 1A Homo sapiens 103-107 11295047-0 2001 Synergistic effects of dexamethasone and genistein on the expression of Cdk inhibitor p21WAF1/CIP1 in human hepatocellular and colorectal carcinoma cells. Genistein 41-50 cyclin dependent kinase inhibitor 1A Homo sapiens 86-98 11410520-6 2001 Cell cycle regulator p21(WAF1), which is known to be up-regulated by genistein treatment, was greatly induced at RNA and protein levels in normal cells, whereas its level was only slightly induced in malignant MDA-MB-231 cells and not detectable in malignant MCF10CA1a cells. Genistein 69-78 cyclin dependent kinase inhibitor 1A Homo sapiens 21-24 11410520-6 2001 Cell cycle regulator p21(WAF1), which is known to be up-regulated by genistein treatment, was greatly induced at RNA and protein levels in normal cells, whereas its level was only slightly induced in malignant MDA-MB-231 cells and not detectable in malignant MCF10CA1a cells. Genistein 69-78 cyclin dependent kinase inhibitor 1A Homo sapiens 25-29 11410520-7 2001 Therefore, we investigated the causal role of p21(WAF1) in the differential sensitivity of genistein among these cell lines. Genistein 91-100 cyclin dependent kinase inhibitor 1A Homo sapiens 46-49 11410520-7 2001 Therefore, we investigated the causal role of p21(WAF1) in the differential sensitivity of genistein among these cell lines. Genistein 91-100 cyclin dependent kinase inhibitor 1A Homo sapiens 50-54 11410520-9 2001 We found that there was a greater degree of cell cycle arrest and apoptosis in p21(WAF1) -/- cells compared with p21(WAF1) +/+ HCT116 cells after genistein treatment. Genistein 146-155 cyclin dependent kinase inhibitor 1A Homo sapiens 79-82 11410520-9 2001 We found that there was a greater degree of cell cycle arrest and apoptosis in p21(WAF1) -/- cells compared with p21(WAF1) +/+ HCT116 cells after genistein treatment. Genistein 146-155 cyclin dependent kinase inhibitor 1A Homo sapiens 83-87 11410520-9 2001 We found that there was a greater degree of cell cycle arrest and apoptosis in p21(WAF1) -/- cells compared with p21(WAF1) +/+ HCT116 cells after genistein treatment. Genistein 146-155 cyclin dependent kinase inhibitor 1A Homo sapiens 117-121 11410520-10 2001 Flow cytometric analysis after genistein treatment showed a significant number of p21(WAF1) -/- cells in the hyperdiploid population, which are probably programmed to die through apoptotic processes. Genistein 31-40 cyclin dependent kinase inhibitor 1A Homo sapiens 82-85 11410520-10 2001 Flow cytometric analysis after genistein treatment showed a significant number of p21(WAF1) -/- cells in the hyperdiploid population, which are probably programmed to die through apoptotic processes. Genistein 31-40 cyclin dependent kinase inhibitor 1A Homo sapiens 86-90 11410520-12 2001 We found that both normal and malignant p21(WAF1) antisense (AS)-expressing clones became more sensitive to G(2)-M arrest after genistein treatment. Genistein 128-137 cyclin dependent kinase inhibitor 1A Homo sapiens 40-43 11410520-12 2001 We found that both normal and malignant p21(WAF1) antisense (AS)-expressing clones became more sensitive to G(2)-M arrest after genistein treatment. Genistein 128-137 cyclin dependent kinase inhibitor 1A Homo sapiens 44-48 11295047-2 2001 A block of cell cycle checkpoint by dexamethasone and genistein correlates with a selective induction of cyclin-dependent kinase (Cdk) inhibitor p21WAF1/CIP1 in a tumor suppressor p53-independent manner and abolishment of Cdk2 phosphorylation. Genistein 54-63 cyclin dependent kinase inhibitor 1A Homo sapiens 153-157 11295047-3 2001 In the present study, the effects of dexamethasone and genistein (both singly and combined) on the expression of p21 in human hepatocellular Hep G2 and colorectal Colo320 HSR carcinoma cells were evaluated. Genistein 55-64 cyclin dependent kinase inhibitor 1A Homo sapiens 113-116 11295047-6 2001 The combined effects of dexamethasone and genistein on the induction of p21 protein and activation of p21 promoter were synergistic in both cell lines. Genistein 42-51 cyclin dependent kinase inhibitor 1A Homo sapiens 72-75 11295047-6 2001 The combined effects of dexamethasone and genistein on the induction of p21 protein and activation of p21 promoter were synergistic in both cell lines. Genistein 42-51 cyclin dependent kinase inhibitor 1A Homo sapiens 102-105 11286989-5 2001 In addition, genistein enhanced the expression of the cell cycle inhibitor p21(waf/cip1) by 10- to 15-fold, increased p21(waf/cip1) association with Cdc2 by 2-fold, and increased the expression of the tumor suppressor p53 by 2.8-fold. Genistein 13-22 cyclin dependent kinase inhibitor 1A Homo sapiens 83-87 11286989-5 2001 In addition, genistein enhanced the expression of the cell cycle inhibitor p21(waf/cip1) by 10- to 15-fold, increased p21(waf/cip1) association with Cdc2 by 2-fold, and increased the expression of the tumor suppressor p53 by 2.8-fold. Genistein 13-22 cyclin dependent kinase inhibitor 1A Homo sapiens 126-130 11286989-8 2001 From these results, we conclude that genistein inhibits the growth of nonneoplastic MCF-10F human breast cells by preventing the G2/M phase transition, induces the expression of the cell cycle inhibitor p21(waf/cip1) as well as its interaction with Cdc2, and inhibits the activity of Cdc2 in a phosphorylation-related manner. Genistein 37-46 cyclin dependent kinase inhibitor 1A Homo sapiens 211-215 11074606-4 2000 Physiologic concentrations of genistein (< or = 20 microM) decreased LNCaP viable cell number in a dose-dependent manner, induced a G(1) cell-cycle block, decreased prostate-specific antigen mRNA expression, and increased p27(KIP1) and p21(WAF1) (mRNA and protein) but had no effect on apoptosis or the mRNA expression of the apoptosis- and cell-cycle-related markers bcl-2, bax, Rb, and proliferating cell nuclear antigen. Genistein 30-39 cyclin dependent kinase inhibitor 1A Homo sapiens 239-242 11170137-8 2001 In other studies, silymarin, genistein or EGCG caused a strong induction of Cip1/p21 (up to 2.4-fold) and Kip1/p27 (up to 150-fold), and a strong decrease in CDK4 (40-90%) but had moderate effect on CDK2, and cyclins D1 and E. Genistein 29-38 cyclin dependent kinase inhibitor 1A Homo sapiens 76-80 11062152-3 2000 In previous studies, genistein was shown to induce apoptosis and cell cycle arrest at G(2)/M in several cancer cell lines in vitro, which is associated with induction of p21(WAF1/CIP1), a universal inhibitor of cyclin-dependent kinases. Genistein 21-30 cyclin dependent kinase inhibitor 1A Homo sapiens 170-173 11062152-3 2000 In previous studies, genistein was shown to induce apoptosis and cell cycle arrest at G(2)/M in several cancer cell lines in vitro, which is associated with induction of p21(WAF1/CIP1), a universal inhibitor of cyclin-dependent kinases. Genistein 21-30 cyclin dependent kinase inhibitor 1A Homo sapiens 174-178 11062152-3 2000 In previous studies, genistein was shown to induce apoptosis and cell cycle arrest at G(2)/M in several cancer cell lines in vitro, which is associated with induction of p21(WAF1/CIP1), a universal inhibitor of cyclin-dependent kinases. Genistein 21-30 cyclin dependent kinase inhibitor 1A Homo sapiens 179-183 11062152-8 2000 Interestingly, genistein induces p21(WAF1/CIP1) expression in galectin-3-expressing BT549 cells, but not in control BT549 cells undergoing apoptosis. Genistein 15-24 cyclin dependent kinase inhibitor 1A Homo sapiens 33-36 11062152-8 2000 Interestingly, genistein induces p21(WAF1/CIP1) expression in galectin-3-expressing BT549 cells, but not in control BT549 cells undergoing apoptosis. Genistein 15-24 cyclin dependent kinase inhibitor 1A Homo sapiens 37-41 11062152-8 2000 Interestingly, genistein induces p21(WAF1/CIP1) expression in galectin-3-expressing BT549 cells, but not in control BT549 cells undergoing apoptosis. Genistein 15-24 cyclin dependent kinase inhibitor 1A Homo sapiens 42-46 11062152-9 2000 Collectively, the results of the present study suggest that galectin-3, at least in part, is a critical determinant for genistein-mediated cell cycle arrest and apoptosis, and genistein induction of p21(WAF1/CIP1) is associated with cell cycle arrest, but not required for apoptosis induction. Genistein 176-185 cyclin dependent kinase inhibitor 1A Homo sapiens 199-202 11062152-9 2000 Collectively, the results of the present study suggest that galectin-3, at least in part, is a critical determinant for genistein-mediated cell cycle arrest and apoptosis, and genistein induction of p21(WAF1/CIP1) is associated with cell cycle arrest, but not required for apoptosis induction. Genistein 176-185 cyclin dependent kinase inhibitor 1A Homo sapiens 203-207 11062152-9 2000 Collectively, the results of the present study suggest that galectin-3, at least in part, is a critical determinant for genistein-mediated cell cycle arrest and apoptosis, and genistein induction of p21(WAF1/CIP1) is associated with cell cycle arrest, but not required for apoptosis induction. Genistein 176-185 cyclin dependent kinase inhibitor 1A Homo sapiens 208-212 11007941-13 2000 Silymarin, genistein, and EGCG also resulted in a significant induction of Cip1/p21 and Kip1/p27 and a decrease in cyclin-dependent kinase (CDK) 4, but a moderate inhibition of CDK2, cyclin D1, and cyclin E was observed. Genistein 11-20 cyclin dependent kinase inhibitor 1A Homo sapiens 75-79 11007941-13 2000 Silymarin, genistein, and EGCG also resulted in a significant induction of Cip1/p21 and Kip1/p27 and a decrease in cyclin-dependent kinase (CDK) 4, but a moderate inhibition of CDK2, cyclin D1, and cyclin E was observed. Genistein 11-20 cyclin dependent kinase inhibitor 1A Homo sapiens 80-83 11074606-4 2000 Physiologic concentrations of genistein (< or = 20 microM) decreased LNCaP viable cell number in a dose-dependent manner, induced a G(1) cell-cycle block, decreased prostate-specific antigen mRNA expression, and increased p27(KIP1) and p21(WAF1) (mRNA and protein) but had no effect on apoptosis or the mRNA expression of the apoptosis- and cell-cycle-related markers bcl-2, bax, Rb, and proliferating cell nuclear antigen. Genistein 30-39 cyclin dependent kinase inhibitor 1A Homo sapiens 243-247 11074606-7 2000 The antiproliferative effects of genistein may be mediated by increased levels of p27(KIP1) and p21(WAF1), which are negative cell-cycle regulators that act as cyclin-dependent kinase inhibitors and that have been recently linked with prostate carcinogenesis. Genistein 33-42 cyclin dependent kinase inhibitor 1A Homo sapiens 96-99 11074606-7 2000 The antiproliferative effects of genistein may be mediated by increased levels of p27(KIP1) and p21(WAF1), which are negative cell-cycle regulators that act as cyclin-dependent kinase inhibitors and that have been recently linked with prostate carcinogenesis. Genistein 33-42 cyclin dependent kinase inhibitor 1A Homo sapiens 100-104 11776565-8 1999 CONCLUSION: The inhibitory effect of genistein on cell proliferation of breast cancer appears to be due to p21WAF1/CIP1 expression and apoptosis through mechanism independent of ER and p53. Genistein 37-46 cyclin dependent kinase inhibitor 1A Homo sapiens 115-119 10912792-7 2000 In fact, the level of p21CIP1 induced by genistein was shown to be insufficient to cause CDK2 inhibition. Genistein 41-50 cyclin dependent kinase inhibitor 1A Homo sapiens 22-29 10761703-7 2000 Genistein also induced the activation of a p21 promoter reporter construct, utilizing a sequence distinct from the p53-binding site. Genistein 0-9 cyclin dependent kinase inhibitor 1A Homo sapiens 43-46 10761703-9 2000 These data suggest that genistein may exert a strong anticarcinogenic effect, and that this effect possibly involves an induction of p21, which inhibits the threshold kinase activities of Cdks and associated cyclins, leading to a G2/M arrest in the cell cycle progression. Genistein 24-33 cyclin dependent kinase inhibitor 1A Homo sapiens 133-136 10761703-5 2000 The inhibitory effects of genistein on cell growth proliferation were associated with a G2/M arrest in cell cycle progression concomitant with a marked inhibition of cyclin B1 and an induction of Cdk inhibitor p21 (WAF1/CIP1) in a p53-independent manner. Genistein 26-35 cyclin dependent kinase inhibitor 1A Homo sapiens 210-213 10761703-5 2000 The inhibitory effects of genistein on cell growth proliferation were associated with a G2/M arrest in cell cycle progression concomitant with a marked inhibition of cyclin B1 and an induction of Cdk inhibitor p21 (WAF1/CIP1) in a p53-independent manner. Genistein 26-35 cyclin dependent kinase inhibitor 1A Homo sapiens 215-224 10761703-6 2000 Following genistein treatment of cells, an increased binding of p21 with Cdk2 and Cdc2 paralleled a significant decrease in Cdc2 and Cdk2 kinase activity with no change in Cdk2 and Cdc2 expression. Genistein 10-19 cyclin dependent kinase inhibitor 1A Homo sapiens 64-67 9673352-3 1998 In a previous study our laboratory has presented evidence that genistein inhibits cell proliferation of breast carcinoma cells, an inhibition which is associated with a specific G2/M arrest, induction of p21WAF/CIP1 expression and apoptosis. Genistein 63-72 cyclin dependent kinase inhibitor 1A Homo sapiens 211-215 9664138-0 1998 Genistein-induced G2/M arrest is associated with the inhibition of cyclin B1 and the induction of p21 in human breast carcinoma cells. Genistein 0-9 cyclin dependent kinase inhibitor 1A Homo sapiens 98-101 9664138-7 1998 Genistein induced expression of p21, and the increased levels of p21 were associated with increased binding of p21 with cdc2 and cdk2. Genistein 0-9 cyclin dependent kinase inhibitor 1A Homo sapiens 32-35 9809990-3 1998 In our previous studies, we found that genistein exerted pronounced antiproliferative effects on both estrogen receptor-positive and -negative human breast carcinoma cells through G2-M arrest, induction of p21WAF1/CIP1 expression, and apoptosis. Genistein 39-48 cyclin dependent kinase inhibitor 1A Homo sapiens 214-218 9809990-8 1998 In these xenograft studies, genistein inhibited tumor growth, stimulated apoptosis, and upregulated p21WAF1/CIP1 expression. Genistein 28-37 cyclin dependent kinase inhibitor 1A Homo sapiens 108-112 9513045-0 1998 Genistein inhibits proliferation similarly in estrogen receptor-positive and negative human breast carcinoma cell lines characterized by P21WAF1/CIP1 induction, G2/M arrest, and apoptosis. Genistein 0-9 cyclin dependent kinase inhibitor 1A Homo sapiens 145-149 9513045-4 1998 Genistein results in a five-to six-fold increase in p21WAF1/CIP1 mRNA levels and a three- to four-fold increase in protein levels, only a 1.5-fold increase in p21WAF1/CIP1 transcription but a three- to six-fold increase in p21WAF1/CIP1 mRNA stability. Genistein 0-9 cyclin dependent kinase inhibitor 1A Homo sapiens 60-64 9513045-4 1998 Genistein results in a five-to six-fold increase in p21WAF1/CIP1 mRNA levels and a three- to four-fold increase in protein levels, only a 1.5-fold increase in p21WAF1/CIP1 transcription but a three- to six-fold increase in p21WAF1/CIP1 mRNA stability. Genistein 0-9 cyclin dependent kinase inhibitor 1A Homo sapiens 167-171 9513045-4 1998 Genistein results in a five-to six-fold increase in p21WAF1/CIP1 mRNA levels and a three- to four-fold increase in protein levels, only a 1.5-fold increase in p21WAF1/CIP1 transcription but a three- to six-fold increase in p21WAF1/CIP1 mRNA stability. Genistein 0-9 cyclin dependent kinase inhibitor 1A Homo sapiens 167-171