PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 22801313-10 2012 CONCLUSION: Genistein could promote the activity of eNOS through increasing phosphorylation eNOS(Ser(1179)) level through PI3K/AKT pathway. Genistein 12-21 nitric oxide synthase 3 Homo sapiens 52-56 26653547-12 2015 Expression of human endothelial nitric oxide synthase is associated with genistein supplementation. Genistein 73-82 nitric oxide synthase 3 Homo sapiens 20-53 22669896-5 2012 However, chemical inhibition of protein kinase A (PKA) or adenoviral transfer of the specific endogenous PKA inhibitor gene completely abolished PKA activity and genistein-stimulated eNOS expression and NO production. Genistein 162-171 nitric oxide synthase 3 Homo sapiens 183-187 22801313-2 2012 This study explored the role of PI3K/AKT signaling during genistein promoted eNOS activation. Genistein 58-67 nitric oxide synthase 3 Homo sapiens 77-81 22801313-7 2012 The effect of genistein on phosphorylation eNOS(Ser(1179)) level was also observed in the presence of LY294002 or NSC154020 (PI3K and AKT inhibitors). Genistein 14-23 nitric oxide synthase 3 Homo sapiens 43-47 22801313-8 2012 RESULTS: The concentration of NO and the expression level of phosphorylation eNOS(Ser(1179)) were significantly increased in ox-LDL + genistein treated cells than ox-LDL treated cells (all P < 0.05), and the peak effects were observed at 15 min, however, eNOS mRNA and non-phosphorylated eNOS protein expression were similar between the two groups (P > 0.05). Genistein 134-143 nitric oxide synthase 3 Homo sapiens 77-81 22801313-8 2012 RESULTS: The concentration of NO and the expression level of phosphorylation eNOS(Ser(1179)) were significantly increased in ox-LDL + genistein treated cells than ox-LDL treated cells (all P < 0.05), and the peak effects were observed at 15 min, however, eNOS mRNA and non-phosphorylated eNOS protein expression were similar between the two groups (P > 0.05). Genistein 134-143 nitric oxide synthase 3 Homo sapiens 258-262 22801313-8 2012 RESULTS: The concentration of NO and the expression level of phosphorylation eNOS(Ser(1179)) were significantly increased in ox-LDL + genistein treated cells than ox-LDL treated cells (all P < 0.05), and the peak effects were observed at 15 min, however, eNOS mRNA and non-phosphorylated eNOS protein expression were similar between the two groups (P > 0.05). Genistein 134-143 nitric oxide synthase 3 Homo sapiens 258-262 22801313-9 2012 Furthermore, the expression level of phosphorylation eNOS(Ser(1179)) was significantly lower in PIK3/AKT inhibitors LY294002 and NSC154020 treated cells compared with ox-LDL + genistein treated cells (all P < 0.05). Genistein 176-185 nitric oxide synthase 3 Homo sapiens 53-57 22801313-10 2012 CONCLUSION: Genistein could promote the activity of eNOS through increasing phosphorylation eNOS(Ser(1179)) level through PI3K/AKT pathway. Genistein 12-21 nitric oxide synthase 3 Homo sapiens 92-96 15655515-8 2005 NO and L-citrulline production by EA.hy926 cells rose up to 1.7-fold of control levels after stimulation with genistein for 48-96 h. From these results, we conclude that the suggested positive effects of soy isoflavones on vascular reactivity may be indeed mediated via a long-term effect on the eNOS system. Genistein 110-119 nitric oxide synthase 3 Homo sapiens 296-300 22125674-1 2011 We compared the effects of genistein and daidzein on the expression of chemokines, cell adhesion molecules (CAMs), and endothelial nitric oxide synthase (eNOS) in tumor necrosis factor (TNF)-alpha-stimulated human umbilical vascular endothelial cells (HUVECs). Genistein 27-36 nitric oxide synthase 3 Homo sapiens 119-152 22125674-6 2011 Pre-treatment with either genistein or daidzein elevated eNOS expression and nitric oxide production disturbed by TNF-alpha exposure. Genistein 26-35 nitric oxide synthase 3 Homo sapiens 57-61 20926919-0 2010 Genistein activates endothelial nitric oxide synthase in broiler pulmonary arterial endothelial cells by an Akt-dependent mechanism. Genistein 0-9 nitric oxide synthase 3 Homo sapiens 20-53 18203895-0 2008 Genistein, a soy phytoestrogen, upregulates the expression of human endothelial nitric oxide synthase and lowers blood pressure in spontaneously hypertensive rats. Genistein 0-9 nitric oxide synthase 3 Homo sapiens 68-101 18203895-6 2008 Genistein, at physiologically achievable concentrations in individuals consuming soy products, enhanced the expression of eNOS and subsequently elevated NO synthesis in both HAEC and HUVEC, with 1-10 micromol/L genistein inducing the maximal effects. Genistein 0-9 nitric oxide synthase 3 Homo sapiens 122-126 18203895-7 2008 However, the effects of genistein on eNOS and NO were not mediated by activation of estrogen signaling or inhibition of tyrosine kinases, 2 known biological actions of genistein. Genistein 24-33 nitric oxide synthase 3 Homo sapiens 37-41 18203895-8 2008 Genistein (1-10 micromol/L) increased eNOS gene expression (1.8- to 2.6-fold of control) and significantly increased eNOS promoter activity of the human eNOS gene in HAEC and HUVEC, suggesting that genistein activates eNOS transcription. Genistein 0-9 nitric oxide synthase 3 Homo sapiens 38-42 18203895-8 2008 Genistein (1-10 micromol/L) increased eNOS gene expression (1.8- to 2.6-fold of control) and significantly increased eNOS promoter activity of the human eNOS gene in HAEC and HUVEC, suggesting that genistein activates eNOS transcription. Genistein 0-9 nitric oxide synthase 3 Homo sapiens 117-121 18203895-8 2008 Genistein (1-10 micromol/L) increased eNOS gene expression (1.8- to 2.6-fold of control) and significantly increased eNOS promoter activity of the human eNOS gene in HAEC and HUVEC, suggesting that genistein activates eNOS transcription. Genistein 0-9 nitric oxide synthase 3 Homo sapiens 117-121 18203895-8 2008 Genistein (1-10 micromol/L) increased eNOS gene expression (1.8- to 2.6-fold of control) and significantly increased eNOS promoter activity of the human eNOS gene in HAEC and HUVEC, suggesting that genistein activates eNOS transcription. Genistein 0-9 nitric oxide synthase 3 Homo sapiens 117-121