PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 31055873-1 2019 Essentials Von Willebrand Factor (VWF) is extensively glycosylated with serial studies demonstrating that these carbohydrate determinants play critical roles in regulating multiple aspects of VWF biology. Carbohydrates 112-124 von Willebrand factor Homo sapiens 11-32 31055873-7 2019 An increasing body of evidence has confirmed that these carbohydrate determinants play critical roles in regulating multiple aspects of VWF biology. Carbohydrates 56-68 von Willebrand factor Homo sapiens 136-139 31055873-1 2019 Essentials Von Willebrand Factor (VWF) is extensively glycosylated with serial studies demonstrating that these carbohydrate determinants play critical roles in regulating multiple aspects of VWF biology. Carbohydrates 112-124 von Willebrand factor Homo sapiens 34-37 31055873-1 2019 Essentials Von Willebrand Factor (VWF) is extensively glycosylated with serial studies demonstrating that these carbohydrate determinants play critical roles in regulating multiple aspects of VWF biology. Carbohydrates 112-124 von Willebrand factor Homo sapiens 192-195 26773038-3 2016 Glycosylation at N1574 has previously been suggested to modulate VWF A2 domain interaction with ADAMTS13 through steric hindrance by the bulky carbohydrate structure. Carbohydrates 143-155 von Willebrand factor Homo sapiens 65-68 30770394-12 2019 In addition, alterations in VWF carbohydrate expression are likely to contribute to quantitative and qualitative variations in VWF levels in the normal population. Carbohydrates 32-44 von Willebrand factor Homo sapiens 28-31 30770394-12 2019 In addition, alterations in VWF carbohydrate expression are likely to contribute to quantitative and qualitative variations in VWF levels in the normal population. Carbohydrates 32-44 von Willebrand factor Homo sapiens 127-130 17090649-2 2007 VWF contains several carbohydrate structures, including O-linked glycans that primarily consist of sialylated T antigen (NeuAc(alpha2-3)Gal-(beta1-3)-[NeuAc(alpha2-6)]GalNAc). Carbohydrates 21-33 von Willebrand factor Homo sapiens 0-3 20590881-5 2010 Three decades of research have revealed that the carbohydrate structures of FVIII and VWF contribute to many of the steps that can be distinguished in the life-cycle of these proteins, including biosynthesis/secretion, function and clearance. Carbohydrates 49-61 von Willebrand factor Homo sapiens 86-89 20590881-7 2010 In addition, the interaction of the FVIII/VWF complex with two families of carbohydrate-binding proteins, i.e. Galectins and Siglecs, and their potential physiological relevance will be discussed. Carbohydrates 75-87 von Willebrand factor Homo sapiens 42-45 16990181-11 2006 These data indicate that endothelial carbohydrate determinants and corresponding ligands (namely, mannose-specific lectins) may be involved in the regulation of production and deposition of vWF. Carbohydrates 37-49 von Willebrand factor Homo sapiens 190-193 17002642-6 2006 ABO(H) carbohydrate antigenic determinants, however, are expressed on the N-linked glycan chains of circulating plasma VWF. Carbohydrates 7-19 von Willebrand factor Homo sapiens 119-122 17002642-7 2006 This review will focus on the carbohydrate structures of VWF and recent studies suggesting that subtle variations in these structures (particularly differences in ABO blood group antigen expression) may have clinically significant effects on VWF proteolysis and clearance. Carbohydrates 30-42 von Willebrand factor Homo sapiens 57-60 10911720-1 2000 Little is known about the carbohydrate structure of the platelet von Willebrand factor (vWf). Carbohydrates 26-38 von Willebrand factor Homo sapiens 65-86 12083507-1 2002 The exact function of the carbohydrate component of von Willebrand factor (VWF) is unknown. Carbohydrates 26-38 von Willebrand factor Homo sapiens 52-73 12083507-1 2002 The exact function of the carbohydrate component of von Willebrand factor (VWF) is unknown. Carbohydrates 26-38 von Willebrand factor Homo sapiens 75-78 10911720-1 2000 Little is known about the carbohydrate structure of the platelet von Willebrand factor (vWf). Carbohydrates 26-38 von Willebrand factor Homo sapiens 88-91 9447247-9 1997 Reaction of r-vWF with carbohydrate-specific lectins demonstrated that r-vWF contained a high proportion of N-glycans composed of mannose, galactose, glucose, N-acetylglucosamine and terminal sialic acid. Carbohydrates 23-35 von Willebrand factor Homo sapiens 73-76 9447247-10 1997 Carbohydrate moities were covalently bound to the protein structure and were quantitatively removed from r-vWF only after protein denaturation. Carbohydrates 0-12 von Willebrand factor Homo sapiens 107-110 8560432-0 1995 Estimation of the carbohydrate moiety of von Willebrand factor in the plasma of patients with subtypes 2a and 2b of von Willebrand disease. Carbohydrates 18-30 von Willebrand factor Homo sapiens 41-62 8560432-3 1995 Whereas recent studies have identified mutations in patients suffering from type 2 vWD, the integrity of the carbohydrate moiety of vWF in these patients is still matter of debate. Carbohydrates 109-121 von Willebrand factor Homo sapiens 132-135 8560432-4 1995 In order to analyse in the plasma milieu the carbohydrate content of plasma vWF from various well-characterized type 2 vWD patients, we developed a colorimetric assay in microtiter plate based on the use of peroxidase-conjugated lectins specific for either alpha 2-6 sialic acid or beta 1-4 galactose. Carbohydrates 45-57 von Willebrand factor Homo sapiens 76-79 3115333-12 1987 Abnormalities in vWF carbohydrate may play a role in impaired primary hemostasis in some patients with von Willebrand"s disease. Carbohydrates 21-33 von Willebrand factor Homo sapiens 17-20 2300925-0 1990 Critical role of the carbohydrate moiety in human von Willebrand factor protein for interactions with type I collagen. Carbohydrates 21-33 von Willebrand factor Homo sapiens 50-71 2300925-1 1990 The ability of von Willebrand factor protein (vWF) to agglutinate platelets with ristocetin depends upon the presence of its highest molecular weight multimers (HMWM) and its intact carbohydrate structure. Carbohydrates 182-194 von Willebrand factor Homo sapiens 15-36 2300925-1 1990 The ability of von Willebrand factor protein (vWF) to agglutinate platelets with ristocetin depends upon the presence of its highest molecular weight multimers (HMWM) and its intact carbohydrate structure. Carbohydrates 182-194 von Willebrand factor Homo sapiens 46-49 2300925-3 1990 The role of the carbohydrate structure of vWF in this function has not been established. Carbohydrates 16-28 von Willebrand factor Homo sapiens 42-45 2300925-9 1990 Therefore, the carbohydrate structure of vWF and, in particular, the penultimate galactose moiety, may be critical for vWF-collagen interactions and for the mediation of primary hemostasis. Carbohydrates 15-27 von Willebrand factor Homo sapiens 41-44 2300925-9 1990 Therefore, the carbohydrate structure of vWF and, in particular, the penultimate galactose moiety, may be critical for vWF-collagen interactions and for the mediation of primary hemostasis. Carbohydrates 15-27 von Willebrand factor Homo sapiens 119-122 34130346-5 2021 Together, these carbohydrate chains account for 20% of VWF monomeric mass, and have been shown to modulate VWF structure, function, and half-life. Carbohydrates 16-28 von Willebrand factor Homo sapiens 55-58 34130346-5 2021 Together, these carbohydrate chains account for 20% of VWF monomeric mass, and have been shown to modulate VWF structure, function, and half-life. Carbohydrates 16-28 von Willebrand factor Homo sapiens 107-110 3128350-0 1988 Adhesive properties of the carbohydrate-modified von Willebrand factor (CHO-vWF). Carbohydrates 27-39 von Willebrand factor Homo sapiens 49-70 3128350-1 1988 In this cooperative study, we explored the role of the carbohydrate moiety (CHO) of von Willebrand factor (vWF) in supporting platelet adhesion. Carbohydrates 55-67 von Willebrand factor Homo sapiens 84-105 3128350-1 1988 In this cooperative study, we explored the role of the carbohydrate moiety (CHO) of von Willebrand factor (vWF) in supporting platelet adhesion. Carbohydrates 55-67 von Willebrand factor Homo sapiens 107-110 1469086-1 1992 By transfecting the full-length cDNA for human von Willebrand factor (vWf) into a line of Chinese hamster ovary cells with a defect in carbohydrate metabolism, we have prepared recombinant vWf specifically lacking O-linked carbohydrates. Carbohydrates 135-147 von Willebrand factor Homo sapiens 47-68 1469086-7 1992 These data indicate a possible role for O-linked carbohydrates in the vWf-glycoprotein 1b interaction promoted by ristocetin and suggest that abnormalities in carbohydrate modification might contribute to the altered ristocetin-dependent reactivity between vWf and platelets described for some variant forms of von Willebrand disease. Carbohydrates 49-61 von Willebrand factor Homo sapiens 70-73 1872845-0 1991 Carbohydrate analysis of human von Willebrand factor with horseradish peroxidase-conjugated lectins. Carbohydrates 0-12 von Willebrand factor Homo sapiens 31-52 2535488-0 1989 Primary structure of the major O-glycosidically linked carbohydrate unit of human von Willebrand factor. Carbohydrates 55-67 von Willebrand factor Homo sapiens 82-103 2460162-2 1988 The extent of large multimer loss was evaluated by examining the sites of subunit cleavage of native and carbohydrate-modified vWF after treatment with trypsin, chymotrypsin, or plasmin. Carbohydrates 105-117 von Willebrand factor Homo sapiens 127-130 2460162-5 1988 Large multimer loss was more extensive with each enzyme after carbohydrate modification of vWF. Carbohydrates 62-74 von Willebrand factor Homo sapiens 91-94 2460162-9 1988 However, digestion of the amino-terminal region was considerably more extensive after carbohydrate modification as judged by a marked decrease or absence of the larger fragments seen when native vWF was digested, and by the appearance of new smaller molecular mass species. Carbohydrates 86-98 von Willebrand factor Homo sapiens 195-198 6752879-18 1982 Several sources of data suggest that carbohydrate is important in vWF-platelet interaction. Carbohydrates 37-49 von Willebrand factor Homo sapiens 66-69 2864750-1 1985 This study compares the ability of unmodified and carbohydrate-modified forms of factor VIII/von Willebrand factor (FVIII/vWF) protein to bind to platelets in the presence of ristocetin or thrombin. Carbohydrates 50-62 von Willebrand factor Homo sapiens 122-125 3000477-5 1986 Since GC contains 60% carbohydrate by weight, we assessed the role of carbohydrate sequences on its interaction with antibody 6D1 and vWF. Carbohydrates 70-82 von Willebrand factor Homo sapiens 134-137 6239876-0 1984 Carbohydrate moiety of von Willebrand factor is not necessary for maintaining multimeric structure and ristocetin cofactor activity but protects from proteolytic degradation. Carbohydrates 0-12 von Willebrand factor Homo sapiens 23-44 6239876-1 1984 To better define the role of carbohydrate in the structure and ristocetin cofactor activity of von Willebrand factor, we have removed up to 83% of total hexose by sequential treatment of the molecule with endo-beta-N-acetyl-glucosaminidase F (endo F), neuraminidase, and beta-galactosidase. Carbohydrates 29-41 von Willebrand factor Homo sapiens 95-116 6239876-5 1984 All multimers of unreduced carbohydrate-modified von Willebrand factor migrated more rapidly in SDS-agarose, but the triplet pattern of individual multimers was unchanged. Carbohydrates 27-39 von Willebrand factor Homo sapiens 49-70 6239876-11 1984 This suggested that carbohydrate was protecting von Willebrand factor against traces of one or more protease contaminants. Carbohydrates 20-32 von Willebrand factor Homo sapiens 48-69 6239876-14 1984 In contrast, the multimeric structure of von Willebrand factor with intact carbohydrate was not affected by plasmin under similar conditions. Carbohydrates 75-87 von Willebrand factor Homo sapiens 41-62 6239876-15 1984 These studies suggest that carbohydrate protects von Willebrand factor from disaggregation occurring secondarily to proteolytic attack but does not play a direct role in maintaining its multimeric structure or ristocetin cofactor activity. Carbohydrates 27-39 von Willebrand factor Homo sapiens 49-70 6805533-4 1982 The PAS stain can detect as little as 300 ng of carbohydrate in the fVIII/vWf protein. Carbohydrates 48-60 von Willebrand factor Homo sapiens 74-77 6805533-8 1982 This difference does not appear to be related to the sialic acid deficiency but may be related to the distribution or organization of the carbohydrate moieties on the smaller fVIII/vWf multimers. Carbohydrates 138-150 von Willebrand factor Homo sapiens 181-184 6800417-4 1982 Comparison of the purified normal and vWd f.VIIi/vWf protein revealed several abnormalities, including decreased concentration of f.VIII/vWf antigen; decreased specific vWf activity; absence of the larger molecular forms of the f.VIII/vWf protein; carbohydrate deficiencies affecting the sialic acid, penultimate galactose and N-acetylglucosamine moieties; and decreased binding of the f.VIII/vWf protein to its platelet receptor. Carbohydrates 248-260 von Willebrand factor Homo sapiens 49-52 6801813-0 1982 Preliminary results on the carbohydrate moiety of factor VIII/von Willebrand factor (FVIII/vWf). Carbohydrates 27-39 von Willebrand factor Homo sapiens 91-94 6801813-3 1982 The carbohydrate moiety of this highly purified FVIII/vWf was submitted to analysis by gas liquid chromatography and thin layer chromatography before and after hydrazinolysis and alkaline-borohydride treatment. Carbohydrates 4-16 von Willebrand factor Homo sapiens 54-57 6801813-10 1982 Thus, the high degree of heterogeneity of the FVIII/vWf carbohydrate moiety requires further structural studies in order to precise which class of glycans is involved in the biological activity of FVIII/vWf. Carbohydrates 56-68 von Willebrand factor Homo sapiens 52-55 6801813-10 1982 Thus, the high degree of heterogeneity of the FVIII/vWf carbohydrate moiety requires further structural studies in order to precise which class of glycans is involved in the biological activity of FVIII/vWf. Carbohydrates 56-68 von Willebrand factor Homo sapiens 203-206 6965350-8 1980 As with ristocetin-induced platelet agglutination, the carbohydrate content plays a significant role in the binding of the factor VIII/von Willebrand factor protein to the platelet. Carbohydrates 55-67 von Willebrand factor Homo sapiens 135-156 301402-1 1977 The effects of several kinds of carbohydrate oxidase, SH-inhibitors and some other chemical reagents on the activities of von Willebrand factor, factor VIII procoagulant and factor VIII-related antigen were studied. Carbohydrates 32-44 von Willebrand factor Homo sapiens 122-201 315413-8 1979 One patient independently reported as having decreased FVIII/vWF carbohydrate was also studied by this technique. Carbohydrates 65-77 von Willebrand factor Homo sapiens 61-64 315413-13 1979 These studies indicate that an absence or decrease of PAS reactive FVIII/vWF carbohydrate is not a consistent abnormality in von Willebrand"s disease. Carbohydrates 77-89 von Willebrand factor Homo sapiens 73-76 1083071-0 1976 Carbohydrate deficiency of the factor VIII/von Willebrand factor Protein in von Willebrand"s disease variants. Carbohydrates 0-12 von Willebrand factor Homo sapiens 43-64