PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 27870715-0 2017 Posttranscriptional Control of PD-L1 Expression by 17beta-Estradiol via PI3K/Akt Signaling Pathway in ERalpha-Positive Cancer Cell Lines. Estradiol 51-67 AKT serine/threonine kinase 1 Homo sapiens 77-80 28098854-3 2017 In this study, we demonstrate that beta-estradiol (E2) treatment increased LSD1 expression via the GPR30/PI3K/AKT pathway in endometrial cancer cells. Estradiol 35-49 AKT serine/threonine kinase 1 Homo sapiens 110-113 27222231-10 2017 In addition, LY294002 inhibited 17beta-estradiol-induced activation of Akt. Estradiol 32-48 AKT serine/threonine kinase 1 Homo sapiens 71-74 27222231-11 2017 The results indicated that 17beta-estradiol regulates SC proliferation by activating PI3K/Akt. Estradiol 27-43 AKT serine/threonine kinase 1 Homo sapiens 90-93 27222231-12 2017 Both GPR30 and Src are involved in 17beta-estradiol-induced phosphorylation of PI3K/Akt. Estradiol 35-51 AKT serine/threonine kinase 1 Homo sapiens 84-87 27349969-8 2016 Taken together, our data suggest that estradiol induces the production of angiogenic factors via a mechanism involving AKT-mediated NF-kappaB activation partly in non-genomic manner without the estrogen receptor. Estradiol 38-47 AKT serine/threonine kinase 1 Homo sapiens 119-122 27222231-0 2017 PI3K/Akt Activated by GPR30 and Src Regulates 17beta-Estradiol-Induced Cultured Immature Boar Sertoli Cells Proliferation. Estradiol 46-62 AKT serine/threonine kinase 1 Homo sapiens 5-8 27222231-4 2017 In the present study, the role of PI3K/Akt on the 17beta-estradiol-induced piglet SC proliferation was explored. Estradiol 50-66 AKT serine/threonine kinase 1 Homo sapiens 39-42 27222231-8 2017 We also found that 17beta-estradiol induced activation of Akt in a time-dependent manner. Estradiol 19-35 AKT serine/threonine kinase 1 Homo sapiens 58-61 27222231-9 2017 Both LY294002 (an inhibitor of PI3K) and 10-DEBC (an inhibitor of Akt) significantly reduced 17beta-estradiol-induced SC proliferation and reduced mRNA and protein expression of Skp2. Estradiol 93-109 AKT serine/threonine kinase 1 Homo sapiens 66-69 27847386-0 2016 17beta-Estradiol Inhibites Tumor Necrosis Factor-alpha Induced Apoptosis of Human Nucleus Pulposus Cells via the PI3K/Akt Pathway. Estradiol 0-16 AKT serine/threonine kinase 1 Homo sapiens 118-121 27255147-0 2016 17beta-Estradiol Reverses Leptin-Inducing Ovarian Cancer Cell Migration by the PI3K/Akt Signaling Pathway. Estradiol 0-16 AKT serine/threonine kinase 1 Homo sapiens 84-87 27188489-30 2016 Estrogen (17beta-estradiol) can promote the proliferation of HaCaT cells by increasing the expression of PCNA via activating ERK/Akt signaling pathway. Estradiol 10-26 AKT serine/threonine kinase 1 Homo sapiens 129-132 26969072-0 2016 Lambda-cyhalothrin disrupts the up-regulation effect of 17beta-estradiol on post-synaptic density 95 protein expression via estrogen receptor alpha-dependent Akt pathway. Estradiol 56-72 AKT serine/threonine kinase 1 Homo sapiens 158-161 26938432-6 2016 Western blot analysis demonstrated the activation of Akt, Erk and upregulation of PCNA in HaCaT cells treated with 17 beta-estradiol. Estradiol 115-132 AKT serine/threonine kinase 1 Homo sapiens 53-56 26938432-8 2016 Upregulation of PCNA expression, elevated proliferation and high S phase fraction of HaCaT cell by 17 beta-estradiol could be reversed by an Akt or Erk inhibitor. Estradiol 99-116 AKT serine/threonine kinase 1 Homo sapiens 141-144 26938432-9 2016 Moreover, Erk inhibition reversed 17 beta-estradiol-induced Akt activation, whereas an Akt inhibitor exhibited no effect on Erk, further suggesting that Erk was on the upstream while Akt on the downstream of the signaling pathway. Estradiol 34-51 AKT serine/threonine kinase 1 Homo sapiens 60-63 26938432-10 2016 CONCLUSION: This study demonstrates that one of the critical mechanisms underlying 17 beta-estradiol promoting skin wound healing is through regulation of keratinocyte proliferation via Erk/Akt signaling pathway. Estradiol 83-100 AKT serine/threonine kinase 1 Homo sapiens 190-193 25216292-4 2014 17-beta estradiol promoted cell invasion, activated phosphorylated AKT in a dose- and time-dependent manner, decreased E-cadherin and increased cytoplasmic alpha-actinin-4 expression. Estradiol 0-17 AKT serine/threonine kinase 1 Homo sapiens 67-70 26884863-0 2015 17beta-estradiol activates mTOR in chondrocytes by AKT-dependent and AKT-independent signaling pathways. Estradiol 0-16 AKT serine/threonine kinase 1 Homo sapiens 51-54 26884863-0 2015 17beta-estradiol activates mTOR in chondrocytes by AKT-dependent and AKT-independent signaling pathways. Estradiol 0-16 AKT serine/threonine kinase 1 Homo sapiens 69-72 25765937-6 2015 17beta-estradiol induced proliferation of hPASMCs via ERalpha activation and this engaged mitogen-activated protein kinase and Akt signalling. Estradiol 0-16 AKT serine/threonine kinase 1 Homo sapiens 127-130 25739982-6 2015 U0126 (5 muM), a specific inhibitor of (MEK1/2), and 10-DEBC (2 muM), a selective inhibitor of AKT, both significantly reduced 17beta-estradiol-induced phosphorylation of mTOR. Estradiol 127-143 AKT serine/threonine kinase 1 Homo sapiens 95-98 25739982-8 2015 These data indicated that 17beta-estradiol enhances Sertoli cell proliferation via mTOR activation, which involves both ERK1/2 and PI3K/AKT signaling. Estradiol 26-42 AKT serine/threonine kinase 1 Homo sapiens 136-139 25701261-5 2015 Treatment with ERK or Akt specific inhibitors, U0126 or LY294002, respectively, suppressed estradiol-induced growth. Estradiol 91-100 AKT serine/threonine kinase 1 Homo sapiens 22-25 25295093-4 2014 Furthermore, treatment with 17beta-estradiol at a concentration of 10-12 M for 24 h increased the expression levels of GRP94 and ER-alpha36, and the phosphorylation levels of Akt at the Ser473 site (Ser473-Akt). Estradiol 28-44 AKT serine/threonine kinase 1 Homo sapiens 175-178 25620476-0 2014 [Estradiol enhances the proliferation and migration of Ishikawa cells by promotion of angiogenesis induced by activation of NF-kappaB via AKT pathway]. Estradiol 1-10 AKT serine/threonine kinase 1 Homo sapiens 138-141 25620476-2 2014 METHODS: Western blot was used to detect the AKT protein expression in Ishikawa cells after stimulation with estradiol, and the effect of AKT inhibitor or ER inhibitor on the activation of AKT. Estradiol 109-118 AKT serine/threonine kinase 1 Homo sapiens 45-48 25620476-9 2014 The NF-kappaB activity was highest after stimulation with 1x10(-6) mol/L estradiol for 30 min to 1 h. AKT inhibitor significantly reduced the NF-kappaB activity (P < 0.05). Estradiol 73-82 AKT serine/threonine kinase 1 Homo sapiens 102-105 25620476-12 2014 CONCLUSIONS: Estradiol induces the production of VEGF and bFGF through activating NF-kappaB via AKT pathway, and enhances the proliferation and migration ability of cancer cells. Estradiol 13-22 AKT serine/threonine kinase 1 Homo sapiens 96-99 24611062-5 2014 Estradiol-induced mTOR phosphorylation was preceded by rapid and transient activation of both extracellular signal-regulated kinase (ERK) and protein kinase B (Akt) and by phosphatase and tensin homolog (PTEN) degradation. Estradiol 0-9 AKT serine/threonine kinase 1 Homo sapiens 160-163 25138535-1 2014 The sex steroid hormone 17beta-estradiol (E2) regulates breast cancer (BC) cell proliferation and migration through the activation of a plethora of signal transduction cascades (e.g., PI3K/AKT activation) starting after E2 binding to the estrogen receptor alpha (ERalpha). Estradiol 24-40 AKT serine/threonine kinase 1 Homo sapiens 189-192 24848710-9 2014 FSH-induced activation of v-akt murine thymoma viral oncogene homolog 3 (AKT) required IGF1R activity, and overexpression of constitutively active AKT rescued the induction of differentiation markers and 17beta-estradiol production by FSH in the presence of the IGF1R inhibitor. Estradiol 204-220 AKT serine/threonine kinase 1 Homo sapiens 28-31 24396487-6 2014 Simultaneous treatment of 10-9 M 17beta-estradiol (E2) with TM may protect SGC7901 cells from endoplasmic reticulum stress-induced apoptosis by counteracting the inhibitory effect of TM on Akt, causing an increase in the phosphorylation of Ser473-Akt. Estradiol 33-49 AKT serine/threonine kinase 1 Homo sapiens 189-192 25189390-4 2014 Moreover, we found that 17beta-estradiol protects osteocytes against apoptosis via the NO/cGMP signaling pathway: type II PKG mediates estradiol-induced activation of the prosurvival kinases Erk and Akt, whereas type I PKG contributes to prosurvival signaling by directly phosphorylating and inactivating the cell death protein BAD. Estradiol 24-40 AKT serine/threonine kinase 1 Homo sapiens 199-202 24396487-7 2014 It was concluded that low concentrations of E2 may counteract endoplasmic reticulum stress-induced inactivation of Akt to block caspase-3-mediated apoptosis. Estradiol 44-46 AKT serine/threonine kinase 1 Homo sapiens 115-118 25189390-4 2014 Moreover, we found that 17beta-estradiol protects osteocytes against apoptosis via the NO/cGMP signaling pathway: type II PKG mediates estradiol-induced activation of the prosurvival kinases Erk and Akt, whereas type I PKG contributes to prosurvival signaling by directly phosphorylating and inactivating the cell death protein BAD. Estradiol 31-40 AKT serine/threonine kinase 1 Homo sapiens 199-202 23761822-10 2013 In conclusion, estradiol- or environmental endocrine disruptor-induced proliferation of human neuroblastoma cells is effectively abolished by genistein, likely in a cell cycle- and Akt pathway-dependent manner. Estradiol 15-24 AKT serine/threonine kinase 1 Homo sapiens 181-184 23758675-0 2013 Interplay between estrogen receptor and AKT in estradiol-induced alternative splicing. Estradiol 47-56 AKT serine/threonine kinase 1 Homo sapiens 40-43 23546602-0 2013 17beta-estradiol activates glucose uptake via GLUT4 translocation and PI3K/Akt signaling pathway in MCF-7 cells. Estradiol 0-16 AKT serine/threonine kinase 1 Homo sapiens 75-78 23581407-5 2013 It was found that in the presence of estradiol, prolactin inhibits prolidase activity and its down-stream signaling proteins: HIF-1alpha, mTOR, AKT and MAPK p-38, while in the absence of estradiol, an opposite effect was observed. Estradiol 37-46 AKT serine/threonine kinase 1 Homo sapiens 144-147 22799881-5 2012 To verify the requirement for the kinase activity of Akt (a downstream target of IGF1R) in the mitogenic action of estradiol, we used shRNA strategy and shRNA-resistant expression vectors. Estradiol 115-124 AKT serine/threonine kinase 1 Homo sapiens 53-56 22825002-3 2012 17beta-estradiol has been reported to simultaneously stimulate protein kinase B (Akt) and adenosine monophosphate-activated protein kinase (AMPK) in ex vivo skeletal muscle. Estradiol 0-16 AKT serine/threonine kinase 1 Homo sapiens 81-84 22825002-5 2012 In this study, we examined whether 17beta-estradiol simultaneously stimulates the activation of AMPK and IRS-1/Akt in 3T3-L1 adipocytes as well as the 17beta-estradiol-ER-induced interaction between the AMPK and IRS-1/Akt pathway in 3T3-L1 adipocytes not exposed to insulin. Estradiol 35-51 AKT serine/threonine kinase 1 Homo sapiens 111-114 22825002-5 2012 In this study, we examined whether 17beta-estradiol simultaneously stimulates the activation of AMPK and IRS-1/Akt in 3T3-L1 adipocytes as well as the 17beta-estradiol-ER-induced interaction between the AMPK and IRS-1/Akt pathway in 3T3-L1 adipocytes not exposed to insulin. Estradiol 151-167 AKT serine/threonine kinase 1 Homo sapiens 218-221 22825002-6 2012 17beta-estradiol (10-7 M) rapidly activated AMPK and IRS-1/Akt in 3T3-L1 adipocytes, while the ER-alpha/beta non-specific antagonist, ICI 182.780 (10 microM), and the AMPK antagonist compound C (20 microM) reversed the estrogen-induced activation of AMPK and tyrosine (Tyr)-IRS-1/Akt in these cells. Estradiol 0-16 AKT serine/threonine kinase 1 Homo sapiens 59-62 22825002-6 2012 17beta-estradiol (10-7 M) rapidly activated AMPK and IRS-1/Akt in 3T3-L1 adipocytes, while the ER-alpha/beta non-specific antagonist, ICI 182.780 (10 microM), and the AMPK antagonist compound C (20 microM) reversed the estrogen-induced activation of AMPK and tyrosine (Tyr)-IRS-1/Akt in these cells. Estradiol 0-16 AKT serine/threonine kinase 1 Homo sapiens 280-283 22825002-8 2012 These results indicate that 17beta-estradiol activates AMPK through an ER and activates Akt through AMPK activation in 3T3-L1 adipocytes, despite the absence of insulin. Estradiol 28-44 AKT serine/threonine kinase 1 Homo sapiens 88-91 22387674-3 2012 The mechanisms of 17beta-estradiol action implicate activation of signaling pathways such as the phosphatidylinositol-3 kinase/Akt and the mitogen-activated protein kinase pathways. Estradiol 18-34 AKT serine/threonine kinase 1 Homo sapiens 127-130 22781117-0 2012 [Effect of GPER on the activation of PI3K/Akt induced by 17beta-estradiol in endometrial carcinoma cells]. Estradiol 57-73 AKT serine/threonine kinase 1 Homo sapiens 42-45 22781117-1 2012 OBJECTIVE: To investigate the expression of G protein-coupled ER (GPER) and ER in the activation of phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) induced by 17beta-estradiol (17beta-E(2))in endometrial carcinoma cells, Ishikawa and HEC-1A. Estradiol 171-187 AKT serine/threonine kinase 1 Homo sapiens 155-158 21763075-6 2012 17beta-Estradiol treatment increased in all the caudate nucleus and putamen pAkt (Ser473)/betaIII-tubulin, pGSK3beta (Ser9)/betaIII-tubulin and in putamen Akt/betaIII-tubulin compared to vehicle-treated monkeys. Estradiol 0-16 AKT serine/threonine kinase 1 Homo sapiens 77-80 21763075-9 2012 These results suggest that 17beta-estradiol activates striatal DA neurotransmission in primates as reflected with increased DAT specific binding and downstream activation of Akt/GSK3 signaling. Estradiol 27-43 AKT serine/threonine kinase 1 Homo sapiens 174-177 21897387-10 2011 CONCLUSION: These results show oestradiol-induced expression of HIF-1alpha, downstream of the ERalpha/c-Src/PI3K/AKT/mTOR pathway in human breast cancer cells. Estradiol 31-41 AKT serine/threonine kinase 1 Homo sapiens 113-116 21722217-2 2012 In neuroblastoma cells, primary neurones in culture and in the brain in vivo, oestradiol activates the phosphoinositide 3-kinase/Akt/glycogen synthase kinase 3 signalling pathway by a mechanism involving oestrogen receptor alpha. Estradiol 78-88 AKT serine/threonine kinase 1 Homo sapiens 129-132 22011313-4 2012 RESULTS & DISCUSSION: Our findings demonstrate that in vitro cadmium-containing QDs induce cellular proliferation, estrogen receptor alpha activation, and biphasic phosphorylation of AKT and ERK1/2, comparable with 17beta-estradiol. Estradiol 219-235 AKT serine/threonine kinase 1 Homo sapiens 187-190 21803156-5 2011 Our current study demonstrates that 17beta-estradiol activates ERK1/2 and Akt at the early stage and induces ERalpha and survivin mRNA at the late stage to modulate its protection via the suppression of caspase-3 activation in PCZ. Estradiol 36-52 AKT serine/threonine kinase 1 Homo sapiens 74-77 21897387-7 2011 The results define a signalling pathway in breast cancer cells whereby oestradiol induces a rapid protein-protein interaction of ERalpha-c-Src-PI3K, resulting in the activation of PI3K/AKT pathway leading to mammalian target of rapamycin (mTOR) phosphorylation. Estradiol 71-81 AKT serine/threonine kinase 1 Homo sapiens 185-188 21278053-9 2011 Lindane did not activate these pathways, but it inhibited estradiol-mediated Akt and ERK1/2 activation. Estradiol 58-67 AKT serine/threonine kinase 1 Homo sapiens 77-80 20644008-8 2010 The ability of estradiol to enhance capillary formation, increase expression of HO-1, and augment phosphorylation of extracellular signal-regulated kinase 1/2, Akt, and vascular endothelial growth factor receptor 2 was mimicked by its cell-impermeable analog BSA estradiol. Estradiol 15-24 AKT serine/threonine kinase 1 Homo sapiens 117-163 20956467-0 2010 17beta-estradiol augments 18F-FDG uptake and glycolysis of T47D breast cancer cells via membrane-initiated rapid PI3K-Akt activation. Estradiol 0-16 AKT serine/threonine kinase 1 Homo sapiens 118-121 19932734-6 2010 In vitro, 17beta-estradiol can promotes cell proliferation in endometriosis by activating PI3K/AKt pathway via an NFkappaB/PTEN-dependent pathway. Estradiol 10-26 AKT serine/threonine kinase 1 Homo sapiens 95-98 20446020-0 2010 17beta-estradiol inhibits prostaglandin E2-induced COX-2 expressions and cell migration by suppressing Akt and ERK1/2 signaling pathways in human LoVo colon cancer cells. Estradiol 0-16 AKT serine/threonine kinase 1 Homo sapiens 103-106 20446020-5 2010 We further observed that 17beta-estradiol treatment inhibits PGE2-induced COX-2 expression and cellular motility via suppressing activation of Akt and ERK1/2 in human LoVo cancer cells. Estradiol 25-41 AKT serine/threonine kinase 1 Homo sapiens 143-146 19778547-3 2010 Estradiol activates in vitro and in vivo the phosphoinositide 3-kinase (PI3K)/Akt signaling pathway in neural cells. Estradiol 0-9 AKT serine/threonine kinase 1 Homo sapiens 78-81 20196785-0 2009 Akt mediates 17beta-estradiol and/or estrogen receptor-alpha inhibition of LPS-induced tumor necresis factor-alpha expression and myocardial cell apoptosis by suppressing the JNK1/2-NFkappaB pathway. Estradiol 13-29 AKT serine/threonine kinase 1 Homo sapiens 0-3 19861407-5 2009 Estradiol promoted cell proliferation and activated erbB2/neu tyrosine kinase, Akt, and mitogen-activated protein kinase signaling exclusively in mammary and breast epithelial cell lines with coexpression of both erbB2 and erbB3. Estradiol 0-9 AKT serine/threonine kinase 1 Homo sapiens 79-82 19519952-6 2009 We discuss the different apoptotic signaling pathways involved in E2 (17beta-estradiol)-induced apoptosis, including the intrinsic and extrinsic apoptosis pathways, the NF-kappaB (nuclear factor-kappa-B)-mediated survival pathway as well as the PI3K (phosphoinositide 3-kinase)/Akt signaling pathway. Estradiol 70-86 AKT serine/threonine kinase 1 Homo sapiens 278-281 19406242-0 2009 Evidence that low-dose, long-term genistein treatment inhibits oestradiol-stimulated growth in MCF-7 cells by down-regulation of the PI3-kinase/Akt signalling pathway. Estradiol 63-73 AKT serine/threonine kinase 1 Homo sapiens 144-147 19372579-11 2009 We conclude that E(2)-induced T47D:A18/PKCalpha tumor regression requires participation of ER-alpha, the extracellular matrix, FasL/FasL ligand, and Akt pathways, allowing the opportunity to explore new predictive markers and therapeutic targets. Estradiol 17-21 AKT serine/threonine kinase 1 Homo sapiens 149-152 19287985-6 2009 The 17beta-estradiol increased HIF-1alpha expression in ovarian cancer cells, and this upregulatory effect was abrogated by Akt inhibitor LY294002 (P<0.05) and Akt siRNA interference (P<0.05), but not affected by MAPK inhibitor PD980059 (P>0.05). Estradiol 4-20 AKT serine/threonine kinase 1 Homo sapiens 124-127 19287985-6 2009 The 17beta-estradiol increased HIF-1alpha expression in ovarian cancer cells, and this upregulatory effect was abrogated by Akt inhibitor LY294002 (P<0.05) and Akt siRNA interference (P<0.05), but not affected by MAPK inhibitor PD980059 (P>0.05). Estradiol 4-20 AKT serine/threonine kinase 1 Homo sapiens 163-166 18378082-1 2008 We previously showed that estradiol prevents neuronal cell death through the activation of Akt and its downstream targets Bad and FKHR. Estradiol 26-35 AKT serine/threonine kinase 1 Homo sapiens 91-94 18767177-4 2008 Similar to 17beta-estradiol, BPA increases the phosphorylation of both extracellular regulated kinase and AKT. Estradiol 11-27 AKT serine/threonine kinase 1 Homo sapiens 106-109 18644889-2 2008 We identified a nuclear export sequence (NES) in ERalpha and show that its export is dependent on both estradiol-mediated phosphatidylinositol-3-kinase (PI3K)/AKT activation and chromosome region maintenance 1 (CRM1). Estradiol 103-112 AKT serine/threonine kinase 1 Homo sapiens 159-162 18644889-8 2008 In conclusion, estradiol-induced AKT-dependent phosphorylation of FKHR drives its association with ERalpha, thereby triggering complex export from the nucleus necessary for initiation of DNA synthesis and S phase entry. Estradiol 15-24 AKT serine/threonine kinase 1 Homo sapiens 33-36 18378082-8 2008 Estradiol prevented the injury-induced decrease in Akt activation and phosphorylation of mTOR and p70S6 kinases, and the subsequent decrease in S6 phosphorylation. Estradiol 0-9 AKT serine/threonine kinase 1 Homo sapiens 51-54 17351066-9 2007 Immunoblot studies demonstrated that E(2) enhances Akt phosphorylation in HCASMC and that wortmannin, an inhibitor of PI3-kinase, attenuated E(2)-stimulated channel activity, NO production, Akt phosphorylation, and estrogen-stimulated coronary relaxation. Estradiol 37-41 AKT serine/threonine kinase 1 Homo sapiens 51-54 18275979-10 2008 It is concluded that Cd, like estradiol, can cause rapid activation of ERK1/2 and AKT and that these signaling events are mediated by possible interaction with membrane ER alpha and GPR30, but not ER beta. Estradiol 30-39 AKT serine/threonine kinase 1 Homo sapiens 82-85 18314151-8 2008 17 beta-Estradiol-macromolecular conjugate decreases phosphorylation level of PKC alpha and Akt, as part of the process to induce myocardial protection against coronary I/R. Estradiol 3-17 AKT serine/threonine kinase 1 Homo sapiens 92-95 18473907-4 2008 Moreover, some experiments have shown that low concentrations of estradiol, induce an increase in the rate of IRS-1 phosphorylation, promotes the association between IRS-1 and the subunit of PI3-k, p85alpha, causes a decrease in the rate of IRS-1 serine phosphorylation and increases the rate of Akt phosphorylation. Estradiol 65-74 AKT serine/threonine kinase 1 Homo sapiens 296-299 18473910-10 2008 The relationship between insulin resistance states associated with aging in females, and the cross-talk between estradiol and proteins includes in the IRS-1/PI3-k/Akt and IGF-1-IR signalling pathways, will lead to a more complete understanding of the precise mechanism underlying estradiol-mediated neuroprotection. Estradiol 112-121 AKT serine/threonine kinase 1 Homo sapiens 163-166 18473910-10 2008 The relationship between insulin resistance states associated with aging in females, and the cross-talk between estradiol and proteins includes in the IRS-1/PI3-k/Akt and IGF-1-IR signalling pathways, will lead to a more complete understanding of the precise mechanism underlying estradiol-mediated neuroprotection. Estradiol 280-289 AKT serine/threonine kinase 1 Homo sapiens 163-166 18000226-4 2007 Furthermore, estradiol and tamoxifen are observed to induce AKT activation. Estradiol 13-22 AKT serine/threonine kinase 1 Homo sapiens 60-63 17250656-0 2007 17-Beta-estradiol-mediated activation of extracellular-signal regulated kinase, phosphatidylinositol 3-kinase/protein kinase B-Akt and N-methyl-D-aspartate receptor phosphorylation in cortical synaptoneurosomes. Estradiol 0-17 AKT serine/threonine kinase 1 Homo sapiens 127-130 17350964-0 2007 PI3K/Akt and ERK1/2 signalling pathways are involved in endometrial cell migration induced by 17beta-estradiol and growth factors. Estradiol 94-110 AKT serine/threonine kinase 1 Homo sapiens 5-8 17350964-7 2007 Similarly, 17beta-estradiol (10(-6)-10(-8) M) could enhance both constitutive and PDGF-induced migration of the cells and their rapid treatment with the hormone significantly increased phosphorylation of ERK1/2 and Akt. Estradiol 11-27 AKT serine/threonine kinase 1 Homo sapiens 215-218 17138652-0 2007 The effect of estradiol on in vivo tumorigenesis is modulated by the human epidermal growth factor receptor 2/phosphatidylinositol 3-kinase/Akt1 pathway. Estradiol 14-23 AKT serine/threonine kinase 1 Homo sapiens 140-144 17138652-9 2007 Immunohistochemical assays showed that in tumors from parental and CMV xenografts, estradiol decreased estrogen receptor-alpha expression and induced progesterone receptor expression and Akt phosphorylation, effects that were inhibited by tamoxifen, AG825, and R25C-Akt1 by 89, 82, and 77% for progesterone receptor expression and 48, 66, and 73% for pAkt expression, respectively. Estradiol 83-92 AKT serine/threonine kinase 1 Homo sapiens 187-190 17138652-9 2007 Immunohistochemical assays showed that in tumors from parental and CMV xenografts, estradiol decreased estrogen receptor-alpha expression and induced progesterone receptor expression and Akt phosphorylation, effects that were inhibited by tamoxifen, AG825, and R25C-Akt1 by 89, 82, and 77% for progesterone receptor expression and 48, 66, and 73% for pAkt expression, respectively. Estradiol 83-92 AKT serine/threonine kinase 1 Homo sapiens 266-270 17194752-3 2007 In this study, we have investigated the role of MNAR in 17beta-estradiol (E2)-induced activation of the phosphatidylinositol 3-kinase (PI3K)/Akt pathway. Estradiol 56-72 AKT serine/threonine kinase 1 Homo sapiens 141-144 17194752-3 2007 In this study, we have investigated the role of MNAR in 17beta-estradiol (E2)-induced activation of the phosphatidylinositol 3-kinase (PI3K)/Akt pathway. Estradiol 74-76 AKT serine/threonine kinase 1 Homo sapiens 141-144 16872778-4 2007 We also summarize the data suggesting a non-classical estrogen receptor may underlie some of the protective effects of E2 by activating cellular signaling pathways, such as extracellular-regulated kinase (ERK) and phosphatidylinositol 3-kinase/Akt. Estradiol 119-121 AKT serine/threonine kinase 1 Homo sapiens 244-247 17097286-1 2007 PTEN phosphatase, a product of PTEN tumor suppressor gene, exists in cells in phosphorylated and unphosphorylated form and has a central role in regulation of PI3K/Akt signalling which is involved in non-genomic action of estradiol. Estradiol 222-231 AKT serine/threonine kinase 1 Homo sapiens 164-167 17085878-0 2006 17beta-estradiol pretreatment prevents the global ischemic injury-induced decrease of Akt activation and bad phosphorylation in gerbils. Estradiol 0-16 AKT serine/threonine kinase 1 Homo sapiens 86-89 17085878-2 2006 This study investigated whether estradiol modulates neuroprotective mechanism through the activation of Akt and its downstream target such as Bad in global ischemic injury. Estradiol 32-41 AKT serine/threonine kinase 1 Homo sapiens 104-107 16609013-6 2006 However, in the presence of E(2), 2ME inhibited E(2)-induced cell growth and prevented E(2)-induced Akt phosphorylation. Estradiol 28-32 AKT serine/threonine kinase 1 Homo sapiens 100-103 17085878-11 2006 Our findings suggest that estradiol prevents cell death due to global ischemic injury and that Akt activation and Bad phosphorylation by estradiol mediated these protective effects. Estradiol 137-146 AKT serine/threonine kinase 1 Homo sapiens 95-98 16971528-6 2006 E2-induced phosphorylation of Akt, was first apparent at 10 min and maximal at 30 min. Estradiol 0-2 AKT serine/threonine kinase 1 Homo sapiens 30-33 16567092-0 2006 17 beta-estradiol activates PI3K/Akt signaling pathway by estrogen receptor (ER)-dependent and ER-independent mechanisms in endometrial cancer cells. Estradiol 3-17 AKT serine/threonine kinase 1 Homo sapiens 33-36 16567092-2 2006 The main aims are to study if PI3K/Akt signaling pathway can be activated by 17beta-estradiol (E2) via non-nuclear action and to investigate the relationship of the action of E2 and ER in endometrial cancer cells expressing with different status of ER. Estradiol 77-93 AKT serine/threonine kinase 1 Homo sapiens 35-38 16537014-0 2006 Blockage of PI3K/PKB/P27kip1 signaling pathway can antagonize 17 beta-estradiol-induced Ishikawa proliferation and cell cycle progression. Estradiol 62-79 AKT serine/threonine kinase 1 Homo sapiens 17-20 16269459-3 2006 E2 enhanced insulin-induced tyrosine phosphorylation of insulin receptor substrate-1 (IRS-1), IRS-1/p85 association, phosphorylation of Akt, and 2-deoxyglucose uptake at 10(-8) m, but inhibited these effects at 10(-5) m. A concentration of 10(-5) m E2 enhanced insulin-induced phosphorylation of IRS-1 at Ser(307), which was abolished by treatment with a c-Jun NH(2)-terminal kinase inhibitor. Estradiol 0-2 AKT serine/threonine kinase 1 Homo sapiens 136-139 16203130-4 2006 Treatment of breast cancer cells and osteosarcoma cells with estradiol, estren, substance A and substance B led to non-genomic activation of Akt (protein kinase B) and extracellular signal-regulated kinase 1/2 (ERK1/2) signaling cascades mediated by Src (Rous Sarcoma Virus, non-receptor tyrosine kinase) and phosphatidylinositol-3-kinase (PI3K) stimulation. Estradiol 61-70 AKT serine/threonine kinase 1 Homo sapiens 141-144 16169518-0 2005 Up-regulation of PI3K/Akt signaling by 17beta-estradiol through activation of estrogen receptor-alpha, but not estrogen receptor-beta, and stimulates cell growth in breast cancer cells. Estradiol 39-55 AKT serine/threonine kinase 1 Homo sapiens 22-25 16144913-8 2005 Interestingly, ERalpha and PPARgamma pathways have an opposite effect on the regulation of the PI3K/AKT transduction cascade, explaining, at least in part, the divergent response exerted by the cognate ligands 17beta-estradiol and BRL49653 on MCF7 cell proliferation. Estradiol 210-226 AKT serine/threonine kinase 1 Homo sapiens 100-103 16093915-10 2005 Because the expression of TERT is regulated by the PI3-K/Akt pathway, we examined the effect of 17beta-estradiol on Akt activity in EPCs. Estradiol 96-112 AKT serine/threonine kinase 1 Homo sapiens 116-119 16093915-11 2005 Immunoblotting analysis revealed that 17beta-estradiol dose-dependently led to phosphorylation and, thus, to activation of Akt in EPCs. Estradiol 38-54 AKT serine/threonine kinase 1 Homo sapiens 123-126 16049923-7 2005 17beta-Estradiol did, however, increase levels of phosphorylated extracellular signal-regulated kinase 1/2 and AKT, suggesting that activation of these prosurvival proteins may represent one mechanism for its neuroprotective action. Estradiol 0-16 AKT serine/threonine kinase 1 Homo sapiens 111-114 15115729-9 2004 Akt phosphorylation by estradiol was inhibited by the PI3K inhibitor, wortmannin, but not by the ER antagonist, ICI 182 780. Estradiol 23-32 AKT serine/threonine kinase 1 Homo sapiens 0-3 15345655-0 2004 17beta-estradiol reduces cardiomyocyte apoptosis in vivo and in vitro via activation of phospho-inositide-3 kinase/Akt signaling. Estradiol 0-16 AKT serine/threonine kinase 1 Homo sapiens 115-118 15115729-10 2004 The maximal effect on Akt activity was observed following 5-15 min of estradiol treatment. Estradiol 70-79 AKT serine/threonine kinase 1 Homo sapiens 22-25 15115729-11 2004 Our results suggest that estradiol may directly affect PI3K-related signaling pathway by increasing the phosphorylation of Akt in endometrial cells. Estradiol 25-34 AKT serine/threonine kinase 1 Homo sapiens 123-126 15465108-3 2004 METHODS AND RESULTS: Treatment of bovine microvascular and human umbilical endothelial cells (HUVEC) with 17-beta-estradiol (E2) (10(-9) to 10(-5)M) increased phosphorylation of Akt within 1 min and this was followed by phosphorylation of eNOS. Estradiol 106-123 AKT serine/threonine kinase 1 Homo sapiens 178-181 15277603-1 2004 Estradiol rapidly activates Akt via the ErbB2 signaling pathway. Estradiol 0-9 AKT serine/threonine kinase 1 Homo sapiens 28-31 15288763-9 2004 We observed that estradiol also stimulates the phosphatidylinositol-3-kinase (PI3K)/AKT pathway in MCF-7 cells [EMBO J. Estradiol 17-26 AKT serine/threonine kinase 1 Homo sapiens 84-87 15048073-7 2004 These results indicate that E(2) induces telomerase activity not only by transcriptional regulation of hTERT via an ERE-dependent mechanism and a PI3K/Akt/NFkappaB cascade, but also by post-transcriptional regulation via Akt-dependent phosphorylation of hTERT. Estradiol 28-32 AKT serine/threonine kinase 1 Homo sapiens 151-154 15048073-7 2004 These results indicate that E(2) induces telomerase activity not only by transcriptional regulation of hTERT via an ERE-dependent mechanism and a PI3K/Akt/NFkappaB cascade, but also by post-transcriptional regulation via Akt-dependent phosphorylation of hTERT. Estradiol 28-32 AKT serine/threonine kinase 1 Homo sapiens 221-224 15001646-9 2004 Upon estradiol exposure, we observed an enhanced phosphorylation of the proteins involved in the phosphatidylinositol-3-OH kinase (PI3K)/Akt pathway like PDK1, Akt, GSK-3, Bcl-2, together with ERK1/2, which was also involved in cell survival signals. Estradiol 5-14 AKT serine/threonine kinase 1 Homo sapiens 137-140 15001646-9 2004 Upon estradiol exposure, we observed an enhanced phosphorylation of the proteins involved in the phosphatidylinositol-3-OH kinase (PI3K)/Akt pathway like PDK1, Akt, GSK-3, Bcl-2, together with ERK1/2, which was also involved in cell survival signals. Estradiol 5-14 AKT serine/threonine kinase 1 Homo sapiens 160-163 12821935-1 2003 In breast cancer cells, 17-beta-estradiol (E2) upregulates the expression of insulin receptor substrate 1 (IRS-1), a molecule transmitting insulin-like growth factor-I (IGF-I) signals through the PI-3K/Akt survival pathways. Estradiol 24-41 AKT serine/threonine kinase 1 Homo sapiens 202-205 12970748-0 2003 Effect of estradiol on estrogen receptor-alpha gene expression and activity can be modulated by the ErbB2/PI 3-K/Akt pathway. Estradiol 10-19 AKT serine/threonine kinase 1 Homo sapiens 113-116 12970748-2 2003 Estradiol can also rapidly activate PI 3-K/Akt in these cells (nongenomic effect). Estradiol 0-9 AKT serine/threonine kinase 1 Homo sapiens 43-46 12970748-3 2003 The recent study examines whether Akt is involved in the ER-alpha regulation by estradiol (genomic effect). Estradiol 80-89 AKT serine/threonine kinase 1 Homo sapiens 34-37 12970748-5 2003 Stable transfection of MCF-7 cells with a constitutively active Akt mimicked the effect of estradiol. Estradiol 91-100 AKT serine/threonine kinase 1 Homo sapiens 64-67 12970748-6 2003 The changes in ER-alpha expression and activity were abrogated in response to estradiol by an arginine to cysteine mutation in the pleckstrin homology (PH) domain of Akt (R25C), suggesting the involvement of this amino acid in the interaction between Akt and ER-alpha. Estradiol 78-87 AKT serine/threonine kinase 1 Homo sapiens 166-169 12970748-6 2003 The changes in ER-alpha expression and activity were abrogated in response to estradiol by an arginine to cysteine mutation in the pleckstrin homology (PH) domain of Akt (R25C), suggesting the involvement of this amino acid in the interaction between Akt and ER-alpha. Estradiol 78-87 AKT serine/threonine kinase 1 Homo sapiens 251-254 12431978-1 2003 17beta-Estradiol activates endothelial nitric oxide synthase (eNOS), enhancing nitric oxide (NO) release from endothelial cells via the phosphatidylinositol 3-kinase (PI3-kinase)/Akt pathway. Estradiol 0-16 AKT serine/threonine kinase 1 Homo sapiens 179-182 12808053-0 2003 Biphasic estradiol-induced AKT phosphorylation is modulated by PTEN via MAP kinase in HepG2 cells. Estradiol 9-18 AKT serine/threonine kinase 1 Homo sapiens 27-30 12808053-4 2003 17beta-Estradiol induced the rapid and biphasic phosphorylation of AKT. Estradiol 0-16 AKT serine/threonine kinase 1 Homo sapiens 67-70 12554767-0 2003 Estradiol rapidly activates Akt via the ErbB2 signaling pathway. Estradiol 0-9 AKT serine/threonine kinase 1 Homo sapiens 28-31 12554767-2 2003 In this report we now show that estradiol can also rapidly activate phosphatidylinositol 3-kinase (PI 3-K)/Akt and that this effect is mediated by the ErbB2 signaling pathway. Estradiol 32-41 AKT serine/threonine kinase 1 Homo sapiens 107-110 12554767-3 2003 Treatment of cells with estradiol resulted in phosphorylation of Akt and a 9-fold increase in Akt activity in 10 min. Estradiol 24-33 AKT serine/threonine kinase 1 Homo sapiens 65-68 12554767-3 2003 Treatment of cells with estradiol resulted in phosphorylation of Akt and a 9-fold increase in Akt activity in 10 min. Estradiol 24-33 AKT serine/threonine kinase 1 Homo sapiens 94-97 12554767-5 2003 Akt activation by estradiol was abrogated by an arginine-to-cysteine mutation in the pleckstrin homology domain of Akt (R25C). Estradiol 18-27 AKT serine/threonine kinase 1 Homo sapiens 0-3 12554767-5 2003 Akt activation by estradiol was abrogated by an arginine-to-cysteine mutation in the pleckstrin homology domain of Akt (R25C). Estradiol 18-27 AKT serine/threonine kinase 1 Homo sapiens 115-118 12554767-7 2003 Transient transfection of ERalpha into these cells restored Akt activation by estradiol, suggesting that estradiol activation of Akt requires the ERalpha. Estradiol 78-87 AKT serine/threonine kinase 1 Homo sapiens 60-63 12554767-7 2003 Transient transfection of ERalpha into these cells restored Akt activation by estradiol, suggesting that estradiol activation of Akt requires the ERalpha. Estradiol 78-87 AKT serine/threonine kinase 1 Homo sapiens 129-132 12554767-7 2003 Transient transfection of ERalpha into these cells restored Akt activation by estradiol, suggesting that estradiol activation of Akt requires the ERalpha. Estradiol 105-114 AKT serine/threonine kinase 1 Homo sapiens 60-63 12554767-7 2003 Transient transfection of ERalpha into these cells restored Akt activation by estradiol, suggesting that estradiol activation of Akt requires the ERalpha. Estradiol 105-114 AKT serine/threonine kinase 1 Homo sapiens 129-132 12554767-9 2003 In vitro kinase assays using immunoprecipitation and anti-Akt1, -Akt2, and -Akt3-specific antibodies demonstrated that Akt1 is activated by estradiol in MCF-7 cells whereas Akt3 is the activated isoform in ER-negative MDA-MB231 cells, implying that selective activation of Akt subtypes plays a role in the actions of estradiol. Estradiol 140-149 AKT serine/threonine kinase 1 Homo sapiens 119-123 12554767-9 2003 In vitro kinase assays using immunoprecipitation and anti-Akt1, -Akt2, and -Akt3-specific antibodies demonstrated that Akt1 is activated by estradiol in MCF-7 cells whereas Akt3 is the activated isoform in ER-negative MDA-MB231 cells, implying that selective activation of Akt subtypes plays a role in the actions of estradiol. Estradiol 140-149 AKT serine/threonine kinase 1 Homo sapiens 58-61 12554767-9 2003 In vitro kinase assays using immunoprecipitation and anti-Akt1, -Akt2, and -Akt3-specific antibodies demonstrated that Akt1 is activated by estradiol in MCF-7 cells whereas Akt3 is the activated isoform in ER-negative MDA-MB231 cells, implying that selective activation of Akt subtypes plays a role in the actions of estradiol. Estradiol 317-326 AKT serine/threonine kinase 1 Homo sapiens 119-123 12554767-10 2003 Taken together, our data suggest that estradiol, bound to membrane ERalpha, interacts with and activates an ErbB dimer containing ErbB2, inducing activation of PI 3-K/Akt. Estradiol 38-47 AKT serine/threonine kinase 1 Homo sapiens 167-170 12588759-5 2003 Furthermore, we characterized the subsequent steps in the activation of the PI3K/Akt signaling cascade, comparing the molecular events that follow insulin or estradiol activation of PI3K. Estradiol 158-167 AKT serine/threonine kinase 1 Homo sapiens 81-84 12431978-5 2003 Consistent with these results, PP2 blocked 17beta-estradiol-induced Akt phosphorylation but did not inhibit NO release from cells transduced with a constitutively active Akt. Estradiol 43-59 AKT serine/threonine kinase 1 Homo sapiens 68-71 12431978-6 2003 PP2 abrogated 17beta-estradiol-induced activation of PI3-kinase, indicating that the PP2-inhibitable kinase is upstream of PI3-kinase and Akt. Estradiol 14-30 AKT serine/threonine kinase 1 Homo sapiens 138-141 12431978-8 2003 Moreover, transfection of kinase-dead c-Src inhibited 17beta-estradiol-induced Akt phosphorylation, whereas constitutively active c-Src increased basal Akt phosphorylation. Estradiol 54-70 AKT serine/threonine kinase 1 Homo sapiens 79-82 12538508-4 2003 Here we show that transfection of ER-positive, HER2 gene-amplified BT-74 cells with an expression vector encoding dominant-negative (K179M) Akt1 partially restored the ability of tamoxifen to inhibit estradiol-stimulated ER reporter activity. Estradiol 200-209 AKT serine/threonine kinase 1 Homo sapiens 140-144 12538508-5 2003 Infection of MCF-7 cells with an adenovirus encoding myristoylated, constitutively active Akt induced ER reporter activity in the absence of estradiol and resulted in tamoxifen resistance of these cells in culture. Estradiol 141-150 AKT serine/threonine kinase 1 Homo sapiens 90-93 11978177-5 2002 Exposure of these cells to 17-beta-oestradiol (E(2)) in the presence of NO gave rise to activation of signalling events additional to those triggered by E(2) alone, namely tyrosine phosphorylation of specific proteins, including the insulin receptor substrate-1, with recruitment to this adapter of the phosphatidylinositol 3"-kinase and persistent activation of Akt (protein kinase B). Estradiol 27-45 AKT serine/threonine kinase 1 Homo sapiens 363-366 12372818-5 2002 In vascular endothelial cells (EC), estradiol (E(2)) activates several kinase cascades, including phosphatidylinositol 3-phosphate (PI3K)/Akt, a signaling pathway that impacts EC biology. Estradiol 36-45 AKT serine/threonine kinase 1 Homo sapiens 138-141 11978177-6 2002 Active Akt, in turn, prevented E(2) from activating p42/44 extracellular signal-regulated kinases (ERK 1/2). Estradiol 31-35 AKT serine/threonine kinase 1 Homo sapiens 7-10 11689445-1 2001 The p85-associated phosphatidylinositol (PI) 3-kinase/Akt pathway mediates the oestradiol-induced S-phase entry and cyclin D1 promoter activity in MCF-7 cells. Estradiol 79-89 AKT serine/threonine kinase 1 Homo sapiens 54-57 11689445-2 2001 Experiments with Src, p85alpha and Akt dominant-negative forms indicate that in oestradiol-treated cells these signalling effectors target the cyclin D1 promoter. Estradiol 80-90 AKT serine/threonine kinase 1 Homo sapiens 35-38 11689445-3 2001 Oestradiol acutely increases PI3-kinase and Akt activities in MCF-7 cells. Estradiol 0-10 AKT serine/threonine kinase 1 Homo sapiens 44-47 10424760-5 1999 Stimulation of AKT1 activity by estradiol and IGF-I was blocked by the antiestrogen ICI 182780 and by the phosphatidylinositol-3-kinase inhibitor wortmannin. Estradiol 32-41 AKT serine/threonine kinase 1 Homo sapiens 15-19 11391701-2 2001 We demonstrated previously that the phosphatidylinositol 3-kinase (PI3-K)/Akt signal transduction pathway plays a pivotal role on the neuroprotection provided by 17beta-estradiol against acute glutamate toxicity. Estradiol 162-178 AKT serine/threonine kinase 1 Homo sapiens 74-77 11391701-7 2001 Western blotting analysis demonstrated that treatment with 17beta-estradiol induced the phosphorylation of Akt within 5 min, which was suppressed by pretreatment with LY294002 and ICI182780. Estradiol 59-75 AKT serine/threonine kinase 1 Homo sapiens 107-110 11391701-8 2001 Furthermore, 17beta-estradiol induced phosphorylation of the cAMP response element binding protein (CREB) at Ser(133) within 15 min and then upregulated Bcl-2 in a PI3-K/Akt-dependent manner. Estradiol 13-29 AKT serine/threonine kinase 1 Homo sapiens 170-173 11391701-9 2001 Because CREB is known to be a transcription factor for Bcl-2, these results suggest that 17beta-estradiol exerts its antiapoptotic effects by CREB phosphorylation and Bcl-2 upregulation via nongenomic activation of the PI3-K/Akt pathway in cultured cortical neurons. Estradiol 89-105 AKT serine/threonine kinase 1 Homo sapiens 225-228 11376126-4 2001 Oestradiol induced expression of IRS-1 results in enhanced tyrosine phosphorylation of IRS-1 after IGF-1 stimulation, followed by enhanced mitogen activated protein kinase, phosphoinositide 3" kinase, and Akt activation. Estradiol 0-10 AKT serine/threonine kinase 1 Homo sapiens 205-208 11375271-8 2001 Phospho-Akt(473) is also localized to the nucleus of cultured cardiomyocytes after exposure to 17beta-estradiol or genistein (a phytoestrogen in soy protein-based diets), and neonatal exposure of litters to genistein elevated nuclear phospho-Akt(473) localization. Estradiol 95-111 AKT serine/threonine kinase 1 Homo sapiens 8-11 11295570-3 2001 It has been convincingly shown that E(2) activates phosphoinositol 3-kinase and protein kinase B/AKT, and stimulates ERK and p38 MAP kinases. Estradiol 36-40 AKT serine/threonine kinase 1 Homo sapiens 97-100 11029403-8 2000 Adenoviral-mediated EA.hy926 transduction confirmed functional involvement of Akt, because a kinase-deficient, dominant-negative Akt abolished E(2)-stimulated NO release. Estradiol 143-147 AKT serine/threonine kinase 1 Homo sapiens 78-81 11029403-8 2000 Adenoviral-mediated EA.hy926 transduction confirmed functional involvement of Akt, because a kinase-deficient, dominant-negative Akt abolished E(2)-stimulated NO release. Estradiol 143-147 AKT serine/threonine kinase 1 Homo sapiens 129-132 10797534-8 2000 Immunoblotting assay revealed that treatment with 17beta-estradiol induced the phosphorylation of Akt/PKB, an effector immediately downstream of PI3-K. Estradiol 50-66 AKT serine/threonine kinase 1 Homo sapiens 98-105 34821461-5 2022 Interestingly, E2 at environmental concentrations (0.1-10 nM) could activate the expression of both ERalpha36 and GPR30, and then stimulate the phosphorylation of ERK1/2 and Akt, resulting in cell growth promotion. Estradiol 15-17 AKT serine/threonine kinase 1 Homo sapiens 174-177 34478807-7 2022 Dual inhibition of AKT and ERK1/2 pathways suppresses the production of proinflammatory cytokines, decreases E2 biosynthesis and signaling, and restores progesterone receptor-B signaling components in the epithelial and stromal cells of the endometrium in a cell-specific manner. Estradiol 109-111 AKT serine/threonine kinase 1 Homo sapiens 19-22 34951372-0 2021 beta-Estradiol Induces Mitochondrial Apoptosis in Cervical Cancer Through the Suppression of AKT/NF-kappaB Signaling Pathway. Estradiol 0-14 AKT serine/threonine kinase 1 Homo sapiens 93-96 34951372-11 2021 beta-Estradiol markedly decreased the expression level of p-AKT and p-NF-kappaB. Estradiol 0-14 AKT serine/threonine kinase 1 Homo sapiens 60-63 34951372-12 2021 CONCLUSION: This study demonstrated that beta-estradiol induces mitochondrial apoptosis in cervical cancer through the suppression of the AKT/NF-kappaB signaling pathway, indicating that beta-estradiol may serve as a potential agent for cervical cancer treatment. Estradiol 41-55 AKT serine/threonine kinase 1 Homo sapiens 138-141 34951372-12 2021 CONCLUSION: This study demonstrated that beta-estradiol induces mitochondrial apoptosis in cervical cancer through the suppression of the AKT/NF-kappaB signaling pathway, indicating that beta-estradiol may serve as a potential agent for cervical cancer treatment. Estradiol 187-201 AKT serine/threonine kinase 1 Homo sapiens 138-141 34461224-10 2021 Simvastatin also inhibited E2 downstream signaling, including ERK and AKT pathways, E2/ER transcriptional activity and E2-responsive genes. Estradiol 27-29 AKT serine/threonine kinase 1 Homo sapiens 70-73 34571963-12 2021 While estradiol consistently reduced the TNFalpha-induced MAPK and Akt phosphorylation, only the inhibition of MAPK, but not Akt, significantly abrogated the migratory actions of TNFalpha. Estradiol 6-15 AKT serine/threonine kinase 1 Homo sapiens 67-70 34575683-5 2021 GPR30 activation by 17beta-estradiol elicited the SRC/EGFR/PI3K/Akt/mTOR signaling pathway. Estradiol 20-36 AKT serine/threonine kinase 1 Homo sapiens 64-67 34575683-11 2021 Collectively, our results indicate that 17beta-estradiol-mediated GPR30 activation elicits the SRC/EGFR/PI3K/Akt/mTOR signaling pathway and promotes p62 phosphorylation. Estradiol 40-56 AKT serine/threonine kinase 1 Homo sapiens 109-112 34503515-14 2021 In contrast, treatment of TRIB3-knockdown cells with an inhibitor of p-Akt (Ser473) resulted in rises in the levels of both p-GSK3beta as well as FSHR expression whereas E2 synthesis fell. Estradiol 170-172 AKT serine/threonine kinase 1 Homo sapiens 71-74 34407537-0 2021 Sex differences and estradiol effects in MAPK and Akt cell signalling across subregions of the hippocampus. Estradiol 20-29 AKT serine/threonine kinase 1 Homo sapiens 50-53 34355701-0 2021 Erratum: 17beta-Estradiol Inhibites Tumor Necrosis Factor-alpha Induced Apoptosis of Human Nucleus Pulposus Cells via the PI3K/Akt Pathway. Estradiol 9-25 AKT serine/threonine kinase 1 Homo sapiens 127-130 34371773-7 2021 Consistently, the expression of ERK, PI3K, AKT, and ERalpha increased following treatment with schisandrol A; this effect was slightly better than that of E2 and was mitigated by ICI. Estradiol 155-157 AKT serine/threonine kinase 1 Homo sapiens 43-46 33991616-6 2021 Mechanistically, cotreatment with obesity-related factors (estradiol, insulin and leptin) promoted nuclear translocation of ACLY through Akt-mediated phosphorylation of ACLY at Ser455. Estradiol 59-68 AKT serine/threonine kinase 1 Homo sapiens 137-140 35425995-20 2022 The levels of phosphorylated (p)-mTOR and p-AKT notably increased in estradiol- and ERalpha agonist-treated infected 16HBE cells. Estradiol 69-78 AKT serine/threonine kinase 1 Homo sapiens 44-47 35137666-0 2022 E2 + norethisterone promotes the PI3K-AKT pathway via PGRMC1 to induce breast cancer cell proliferation. Estradiol 0-2 AKT serine/threonine kinase 1 Homo sapiens 38-41 35137666-1 2022 OBJECTIVE: This study aimed to find evidence that progesterone receptor membrane component 1 (PGRMC1) promotes estradiol (E2) + norethisterone (NET)-induced breast cancer proliferation through activation of the phosphatidylinositol-3-kinase (PI3K)-AKT pathway. Estradiol 122-124 AKT serine/threonine kinase 1 Homo sapiens 248-251 35055130-10 2022 The obtained results indicate that chemerin affected P4 and E2 synthesis through the Erk1/2 and Akt signalling pathways. Estradiol 60-62 AKT serine/threonine kinase 1 Homo sapiens 96-99 33790784-4 2021 Within a certain concentration range, 17beta-estradiol induced oxidative stress and apoptosis of HepG2 cells, downregulated ERalpha-36 expression, and increased Akt and Foxo3a phosphorylation. Estradiol 38-54 AKT serine/threonine kinase 1 Homo sapiens 161-164 31809475-0 2020 17beta-Estradiol Prevents Extracellular Matrix Degradation by Downregulating MMP3 Expression via PI3K/Akt/FOXO3 Pathway. Estradiol 0-16 AKT serine/threonine kinase 1 Homo sapiens 102-105 33665661-12 2021 These results suggest that E2 may inhibit IL-1beta-induced NPCs" apoptosis through the PI3K/Akt/mTOR signaling pathway. Estradiol 27-29 AKT serine/threonine kinase 1 Homo sapiens 92-95 33585221-7 2020 In the presence of estradiol, PI3K/AKT/mTOR pathway activation levels were higher in ERbeta1/5-expressing cells than those in ERbeta1-expressing cells. Estradiol 19-28 AKT serine/threonine kinase 1 Homo sapiens 35-38 33466512-6 2021 The proliferation of MCF-7/Akt cells is partially independent of estradiol (E2) and exhibits an incomplete response to the anti-estrogen agent 4-hydroxytamoxifen, demonstrating the resistance of these cells to hormone therapy. Estradiol 65-74 AKT serine/threonine kinase 1 Homo sapiens 27-30 33466512-6 2021 The proliferation of MCF-7/Akt cells is partially independent of estradiol (E2) and exhibits an incomplete response to the anti-estrogen agent 4-hydroxytamoxifen, demonstrating the resistance of these cells to hormone therapy. Estradiol 76-78 AKT serine/threonine kinase 1 Homo sapiens 27-30 32628929-0 2020 beta-estradiol adjusts intestinal function via ERbeta and GPR30 mediated PI3K/AKT signaling activation to alleviate postmenopausal dyslipidemia. Estradiol 0-14 AKT serine/threonine kinase 1 Homo sapiens 78-81 32628929-7 2020 In Caco-2 cells incubated with cholesterol, beta-estradiol up-regulated PI3K/AKT signaling, reduced cholesterol accumulation, suppressed inflammatory signaling, and increased the expression of tight junction-related proteins. Estradiol 44-58 AKT serine/threonine kinase 1 Homo sapiens 77-80 32628929-9 2020 PI3K/AKT inhibition abolished the protective effect of beta-estradiol on cholesterol accumulation, tight junction-related protein expression, and inflammation, but had no influence on ERalpha, ERbeta or GPR30 expression in cholesterol incubated Caco-2 cells. Estradiol 55-69 AKT serine/threonine kinase 1 Homo sapiens 5-8 32628929-10 2020 Our results provide evidence that beta-estradiol regulates intestinal function via ERbeta and GPR30 mediated PI3K/AKT signaling activation to alleviate postmenopausal dyslipidemia. Estradiol 34-48 AKT serine/threonine kinase 1 Homo sapiens 114-117 32665986-10 2020 Pharmacological inhibition of PI3K/Akt by LY294002 abrogated E2-induced expression of PPARgamma (0.24 +-0.09-fold; n = 3; p vs. E2 = 0.0017). Estradiol 61-63 AKT serine/threonine kinase 1 Homo sapiens 35-38 31809475-13 2020 Furthermore, 17beta-estradiol-induced the activation of PI3K/Akt pathway is required for FOXO3 phosphorylated. Estradiol 13-29 AKT serine/threonine kinase 1 Homo sapiens 61-64 32036721-12 2021 Conclusion: Combination of Resveratrol and 17beta-estradiol efficiently resisted IL-1beta induced apoptosis of NP cell, mainly through PI3K/AKT/mTOR/caspase-3 and PI3K/AKT/GSK-3beta pathway. Estradiol 43-59 AKT serine/threonine kinase 1 Homo sapiens 140-143 32036721-0 2021 Resveratrol Combined with 17beta-Estradiol Prevents IL-1beta Induced Apoptosis in Human Nucleus Pulposus Via The PI3K/AKT/Mtor and PI3K/AKT/GSK-3beta Pathway. Estradiol 26-42 AKT serine/threonine kinase 1 Homo sapiens 118-121 32036721-12 2021 Conclusion: Combination of Resveratrol and 17beta-estradiol efficiently resisted IL-1beta induced apoptosis of NP cell, mainly through PI3K/AKT/mTOR/caspase-3 and PI3K/AKT/GSK-3beta pathway. Estradiol 43-59 AKT serine/threonine kinase 1 Homo sapiens 168-171 29608013-9 2018 RESULTS In 17beta-estradiol-treated ATDC5 chondrocytes, increased expression of GPER/GPR30 was found, but fewer mitophagosomes were observed, and decreased numbers of TOM20-positive granules were co-localized with decreased LAMP2 and increased expression levels of TOM20, Hsp60, p-Akt, and p-mTOR, and reduced expression of LC3-II, were found. Estradiol 11-27 AKT serine/threonine kinase 1 Homo sapiens 281-284 32036721-2 2021 Our previous study revealed that Resveratrol (RSV) combined with 17beta-estradiol (E2) was more effective in cutting down IL-1beta induced NP cell apoptosis via PI3K/AKT pathway. Estradiol 65-81 AKT serine/threonine kinase 1 Homo sapiens 166-169 31433456-4 2019 Exposure to 17beta-estradiol (E2) for 15-60 min led to sequential phosphorylation of signaling molecules in MAPK and AKT pathways, IGF1R, EGFR and ERalpha, thus documenting an intact membrane signalosome that activates diverse downstream cascades. Estradiol 12-28 AKT serine/threonine kinase 1 Homo sapiens 117-120 31680862-8 2019 Estradiol treatment increased the level of MAP1A through the phosphorylation of ERK1/2 and increased the level of hemoglobin alpha through the phosphorylation of AKT. Estradiol 0-9 AKT serine/threonine kinase 1 Homo sapiens 162-165 30584245-7 2018 Next, 17beta-estradiol (E2) treatment could improve miR-200c and drop the PTEN level, which caused a consequential increase of the phospho-PI3K-AKT pathway genes. Estradiol 6-22 AKT serine/threonine kinase 1 Homo sapiens 144-147 30584245-7 2018 Next, 17beta-estradiol (E2) treatment could improve miR-200c and drop the PTEN level, which caused a consequential increase of the phospho-PI3K-AKT pathway genes. Estradiol 24-26 AKT serine/threonine kinase 1 Homo sapiens 144-147 29330129-0 2018 Estradiol up-regulates L-type Ca2+ channels via membrane-bound estrogen receptor/phosphoinositide-3-kinase/Akt/cAMP response element-binding protein signaling pathway. Estradiol 0-9 AKT serine/threonine kinase 1 Homo sapiens 107-110 29686616-0 2018 Estradiol Activates PI3K/Akt/GSK3 Pathway Under Chronic Neurodegenerative Conditions Triggered by Perinatal Asphyxia. Estradiol 0-9 AKT serine/threonine kinase 1 Homo sapiens 25-28 29686616-5 2018 We demonstrated that estradiol treatment of adult PA exposed animals induced an increase in estrogen receptor (ER) alpha and insulin-like growth factor receptor (IGF-1R) protein levels, an activation of the phosphatidylinositol 3-kinase/Akt/glycogen synthase kinase 3 beta/beta-catenin signaling pathway and an increase in Bcl-2/Bax ratio in the hippocampus in comparison to PA exposed animals treated with vehicle. Estradiol 21-30 AKT serine/threonine kinase 1 Homo sapiens 237-240 29608013-11 2018 CONCLUSIONS Treatment with 17beta-estradiol protected ATDC5 chondrocytes against mitophagy via the GPER/GPR30 and the PI3K/Akt signaling pathway. Estradiol 27-43 AKT serine/threonine kinase 1 Homo sapiens 123-126 29282029-7 2017 Estradiol or insulin stimulation increased the pAkt/Akt and pS6/S6 ratios, expression of p-SREBP-1, m-SREBP-1, and FASN, and cell proliferation, and these effects were decreased by DHA. Estradiol 0-9 AKT serine/threonine kinase 1 Homo sapiens 48-51 27689466-5 2017 The proliferation assay showed that 17beta-estradiol (E2) induced cell proliferation via activation of Akt and extracellular signal-regulated kinase (ERK) cascades, while shRNA mediated downregulation of ERalpha inhibited the cell proliferation. Estradiol 36-52 AKT serine/threonine kinase 1 Homo sapiens 103-106 27689466-5 2017 The proliferation assay showed that 17beta-estradiol (E2) induced cell proliferation via activation of Akt and extracellular signal-regulated kinase (ERK) cascades, while shRNA mediated downregulation of ERalpha inhibited the cell proliferation. Estradiol 54-56 AKT serine/threonine kinase 1 Homo sapiens 103-106 28440394-14 2017 Furthermore, the levels of eNOS and Akt in endothelial cells were also significantly increased following treatment with 17-beta-estradiol under H/R conditions. Estradiol 120-137 AKT serine/threonine kinase 1 Homo sapiens 36-39