PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 11897696-2 2002 We demonstrated that, as in alpha T(3)-1 cells, in a more differentiated gonadotrope cell line L beta T(2) the GnRH-receptor coupling (PLC, PLA2, PLD) generated second messengers essential for PKCs activation; the characterized isoforms (alpha, beta II, delta, epsilon, zeta) were selectively and differentially down-regulated by TPA (alpha, beta II, delta, epsilon) or GnRH (delta, epsilon). Tetradecanoylphorbol Acetate 330-333 gonadotropin releasing hormone 1 Rattus norvegicus 111-115 11897696-3 2002 In whole cell lysates, proteasome inhibitors (proteasome inhibitor I and II, Lactacystin, beta-Lactone, Calpain inhibitor I) prevented in both gonadotrope cell lines the TPA-induced depletion of PKC alpha, epsilon, and the GnRH-elicited PKC epsilon down-regulation; they counteracted in mixed pituitary cell cultures as well, the TPA-evoked PKC alpha, epsilon depletion. Tetradecanoylphorbol Acetate 170-173 gonadotropin releasing hormone 1 Rattus norvegicus 223-227 10971817-3 2000 The secretion of both forms of GnRH was increased in a dependent manner during depolarization by high K+ solutions, and was stimulated by forskolin and 12-O-tetradecanoylphorbol-13-acetate (TPA), activators of adenylate cyclase and protein kinase C pathways, respectively. Tetradecanoylphorbol Acetate 152-188 gonadotropin releasing hormone 1 Rattus norvegicus 31-35 10971817-3 2000 The secretion of both forms of GnRH was increased in a dependent manner during depolarization by high K+ solutions, and was stimulated by forskolin and 12-O-tetradecanoylphorbol-13-acetate (TPA), activators of adenylate cyclase and protein kinase C pathways, respectively. Tetradecanoylphorbol Acetate 190-193 gonadotropin releasing hormone 1 Rattus norvegicus 31-35 8090283-2 1994 Previously we demonstrated that the synthetic diacylglycerol, phorbol 12-myristate 13-acetate (PMA) and PLC mimic the stimulatory effects of GnRH on both luteinizing hormone (LH) glycosylation and release. Tetradecanoylphorbol Acetate 62-93 gonadotropin releasing hormone 1 Rattus norvegicus 141-145 10433227-6 1999 Treatment with PMA also facilitated LHRH-induced LH release, to approximately the same degree as NPY. Tetradecanoylphorbol Acetate 15-18 gonadotropin releasing hormone 1 Rattus norvegicus 36-40 8838143-1 1996 Treatment of GT1-7 neuronal cells with the phorbol ester, 12-O-tetradecanoyl phorbol 13-acetate (TPA), inhibits GnRH gene transcription. Tetradecanoylphorbol Acetate 58-95 gonadotropin releasing hormone 1 Rattus norvegicus 112-116 8838143-1 1996 Treatment of GT1-7 neuronal cells with the phorbol ester, 12-O-tetradecanoyl phorbol 13-acetate (TPA), inhibits GnRH gene transcription. Tetradecanoylphorbol Acetate 97-100 gonadotropin releasing hormone 1 Rattus norvegicus 112-116 8838143-6 1996 Deletion analysis mapped the region that mediates repression by AP-1 to the area between -126 and -73 base pairs (bp) of the rGnRH 5"-flanking region: the same area that mediates TPA-induced repression and contains an imperfect TPA response element sequence at -99. Tetradecanoylphorbol Acetate 179-182 gonadotropin releasing hormone 1 Rattus norvegicus 125-130 8838143-6 1996 Deletion analysis mapped the region that mediates repression by AP-1 to the area between -126 and -73 base pairs (bp) of the rGnRH 5"-flanking region: the same area that mediates TPA-induced repression and contains an imperfect TPA response element sequence at -99. Tetradecanoylphorbol Acetate 228-231 gonadotropin releasing hormone 1 Rattus norvegicus 125-130 7478915-4 1995 However, PMA or phorbol 12,13-dibutyrate (PdBu), but not the inactive 4alpha-phorbol 12,13-didecanoate (4alpha-PDD), reduced the frequency of GnRH-induced [Ca2+]i oscillation and augmented the IK(Ca) induced by any given level of [Ca2+]i. Tetradecanoylphorbol Acetate 9-12 gonadotropin releasing hormone 1 Rattus norvegicus 142-146 8090283-2 1994 Previously we demonstrated that the synthetic diacylglycerol, phorbol 12-myristate 13-acetate (PMA) and PLC mimic the stimulatory effects of GnRH on both luteinizing hormone (LH) glycosylation and release. Tetradecanoylphorbol Acetate 95-98 gonadotropin releasing hormone 1 Rattus norvegicus 141-145 8142369-4 1994 Addition of the protein kinase C (PKC) activator, 12-O-tetradecanoylphorbol 13-acetate (TPA), or the Ca2+ ionophore, ionomycin, mimicked the profile of GnRH-induced alpha and LH beta mRNA elevation. Tetradecanoylphorbol Acetate 88-91 gonadotropin releasing hormone 1 Rattus norvegicus 152-156 8142369-5 1994 The two phases of FSH beta mRNA elevation induced by GnRH could be mimicked by TPA, while the decrease at 6 h was mimicked by ionomycin. Tetradecanoylphorbol Acetate 79-82 gonadotropin releasing hormone 1 Rattus norvegicus 53-57 8206325-3 1994 Analysis of the half-life of GnRH mRNA levels after transcriptional arrest with actinomycin-D (5 micrograms/ml) estimated the half-life of GnRH mRNA to be 22 h. TPA treatment did not alter the GnRH mRNA half-life directly, suggesting that the effects of TPA occur predominantly at the level of gene transcription. Tetradecanoylphorbol Acetate 161-164 gonadotropin releasing hormone 1 Rattus norvegicus 29-33 2473448-6 1989 Depolarization-induced LH-RH release also did not occur in the presence of 10(-4) or 10(-6) M norepinephrine, 10(-7) M 12-O-tetradecanoylphorbol-13-acetate (TPA, PMA), 10(-5) M forskolin or in female rats. Tetradecanoylphorbol Acetate 157-160 gonadotropin releasing hormone 1 Rattus norvegicus 23-28 8142369-4 1994 Addition of the protein kinase C (PKC) activator, 12-O-tetradecanoylphorbol 13-acetate (TPA), or the Ca2+ ionophore, ionomycin, mimicked the profile of GnRH-induced alpha and LH beta mRNA elevation. Tetradecanoylphorbol Acetate 50-86 gonadotropin releasing hormone 1 Rattus norvegicus 152-156 1661233-7 1991 The potent GnRH antagonist [Ac-D-p-Cl-Phe1.2, D-Trp3, D-Lys6, D-Ala10]GnRH, which completely inhibited GnRHa-stimulated LH release with ID50 of 6.8 nM, also reduced maximum TPA-stimulated LH release by about 50%. Tetradecanoylphorbol Acetate 173-176 gonadotropin releasing hormone 1 Rattus norvegicus 11-15 1661233-7 1991 The potent GnRH antagonist [Ac-D-p-Cl-Phe1.2, D-Trp3, D-Lys6, D-Ala10]GnRH, which completely inhibited GnRHa-stimulated LH release with ID50 of 6.8 nM, also reduced maximum TPA-stimulated LH release by about 50%. Tetradecanoylphorbol Acetate 173-176 gonadotropin releasing hormone 1 Rattus norvegicus 70-74 1661233-10 1991 Furthermore, the partial inhibition of TPA-stimulated LH release by a GnRH antagonist suggests that the pathway(s), specifically connected with LH release in the diverse effects of C kinase, might be locked by the continuous receptor inactivation by antagonist and indicates the complicated pathways which diverge from the receptor and converge into specific cellular response. Tetradecanoylphorbol Acetate 39-42 gonadotropin releasing hormone 1 Rattus norvegicus 70-74 2116958-3 1990 It is thought that this paradoxical phenomenon occurred due to desensitization accompanied by down-regulation of LH-RH receptors induced by TPA. Tetradecanoylphorbol Acetate 140-143 gonadotropin releasing hormone 1 Rattus norvegicus 113-118 2116958-4 1990 This hypothesis was supported by the finding indicating that the binding capacity of LH-RH receptors decreased in a time-course manner during incubation with TPA. Tetradecanoylphorbol Acetate 158-161 gonadotropin releasing hormone 1 Rattus norvegicus 85-90 8206325-3 1994 Analysis of the half-life of GnRH mRNA levels after transcriptional arrest with actinomycin-D (5 micrograms/ml) estimated the half-life of GnRH mRNA to be 22 h. TPA treatment did not alter the GnRH mRNA half-life directly, suggesting that the effects of TPA occur predominantly at the level of gene transcription. Tetradecanoylphorbol Acetate 161-164 gonadotropin releasing hormone 1 Rattus norvegicus 139-143 8206325-3 1994 Analysis of the half-life of GnRH mRNA levels after transcriptional arrest with actinomycin-D (5 micrograms/ml) estimated the half-life of GnRH mRNA to be 22 h. TPA treatment did not alter the GnRH mRNA half-life directly, suggesting that the effects of TPA occur predominantly at the level of gene transcription. Tetradecanoylphorbol Acetate 161-164 gonadotropin releasing hormone 1 Rattus norvegicus 139-143 8206325-4 1994 Exposure of cells transiently transfected with various deletion constructs of the rat (r)GnRH promoter to TPA resulted in a decrease of 60% in luciferase reporter activity. Tetradecanoylphorbol Acetate 106-109 gonadotropin releasing hormone 1 Rattus norvegicus 89-93 8472844-11 1993 Progesterone modulation of GnRH-stimulated FSH secretion was multiphasic, i.e. increased at 0-6 h, unchanged at 9 h and suppressed at 24 h. Acute and chronic exposures to P similarly modulated A23187-stimulated FSH release, whereas both P treatments increased PMA-stimulated FSH secretion. Tetradecanoylphorbol Acetate 260-263 gonadotropin releasing hormone 1 Rattus norvegicus 27-31 8485613-7 1993 Both of the biphasic actions of GnRH on aromatase response to FSH were mimicked by protein kinase C activators, phobol myristate acetate (PMA) and oleoylacetyl glycerol; maximal effects occurred at 1 to 10 ng/mL. Tetradecanoylphorbol Acetate 138-141 gonadotropin releasing hormone 1 Rattus norvegicus 32-36 1657576-12 1991 Treatment of pituitary cell cultures with PMA (8-16 h) also stimulated the synthesis of GnRH receptors, although to a lesser extent than that observed after GnRH treatment. Tetradecanoylphorbol Acetate 42-45 gonadotropin releasing hormone 1 Rattus norvegicus 88-92 2170797-8 1990 In vitro perfusion of hypothalamic fragments with PMA and/or FKN stimulated LHRH release as well as LHRH mRNA. Tetradecanoylphorbol Acetate 50-53 gonadotropin releasing hormone 1 Rattus norvegicus 76-80 2170797-8 1990 In vitro perfusion of hypothalamic fragments with PMA and/or FKN stimulated LHRH release as well as LHRH mRNA. Tetradecanoylphorbol Acetate 50-53 gonadotropin releasing hormone 1 Rattus norvegicus 100-104 2170797-9 1990 The combined infusion of FKN and PMA did not produce an additive effect on the LHRH mRNA levels, but it was effective in synergistically increasing LHRH secretion in vitro. Tetradecanoylphorbol Acetate 33-36 gonadotropin releasing hormone 1 Rattus norvegicus 148-152 2473448-6 1989 Depolarization-induced LH-RH release also did not occur in the presence of 10(-4) or 10(-6) M norepinephrine, 10(-7) M 12-O-tetradecanoylphorbol-13-acetate (TPA, PMA), 10(-5) M forskolin or in female rats. Tetradecanoylphorbol Acetate 162-165 gonadotropin releasing hormone 1 Rattus norvegicus 23-28 3139993-8 1988 Like GnRH, the protein kinase C activator phorbol myristate acetate also induced tPA mRNA in granulosa cells. Tetradecanoylphorbol Acetate 42-67 gonadotropin releasing hormone 1 Rattus norvegicus 5-9 2843535-4 1988 The protein kinase C activator, phorbol 12-myristate 13-acetate, at concentrations (2-20 nM) which did not deplete protein kinase C, stimulated LH-beta mRNA levels 2-5-fold after 24 h. Higher concentrations of phorbol 12-myristate 13-acetate, which depleted protein kinase C activity, substantially reduced the ability of 100 pM GnRH to stimulate increases in LH-beta mRNA levels. Tetradecanoylphorbol Acetate 32-63 gonadotropin releasing hormone 1 Rattus norvegicus 329-333 3114254-6 1987 The GnRH effect was mimicked by the calcium- and phospholipid-dependent protein kinase C activator, phorbol myristate acetate. Tetradecanoylphorbol Acetate 100-125 gonadotropin releasing hormone 1 Rattus norvegicus 4-8 3009147-3 1986 Activation of protein kinase C with phorbol 12-myristate 13-acetate (PMA) results 2 h later in an amplification of LHRH-induced LH secretion in a concentration (1 nM to 1 microM)-and protein synthesis-dependent manner in proestrous, but not estrous, pituitaries; the diacylglycerol analog 1-oleoyl-2-acetylglycerol (OAG) also augments subsequent LHRH-induced secretion. Tetradecanoylphorbol Acetate 36-67 gonadotropin releasing hormone 1 Rattus norvegicus 115-119 3009147-3 1986 Activation of protein kinase C with phorbol 12-myristate 13-acetate (PMA) results 2 h later in an amplification of LHRH-induced LH secretion in a concentration (1 nM to 1 microM)-and protein synthesis-dependent manner in proestrous, but not estrous, pituitaries; the diacylglycerol analog 1-oleoyl-2-acetylglycerol (OAG) also augments subsequent LHRH-induced secretion. Tetradecanoylphorbol Acetate 36-67 gonadotropin releasing hormone 1 Rattus norvegicus 346-350 3009147-3 1986 Activation of protein kinase C with phorbol 12-myristate 13-acetate (PMA) results 2 h later in an amplification of LHRH-induced LH secretion in a concentration (1 nM to 1 microM)-and protein synthesis-dependent manner in proestrous, but not estrous, pituitaries; the diacylglycerol analog 1-oleoyl-2-acetylglycerol (OAG) also augments subsequent LHRH-induced secretion. Tetradecanoylphorbol Acetate 69-72 gonadotropin releasing hormone 1 Rattus norvegicus 115-119 3009147-3 1986 Activation of protein kinase C with phorbol 12-myristate 13-acetate (PMA) results 2 h later in an amplification of LHRH-induced LH secretion in a concentration (1 nM to 1 microM)-and protein synthesis-dependent manner in proestrous, but not estrous, pituitaries; the diacylglycerol analog 1-oleoyl-2-acetylglycerol (OAG) also augments subsequent LHRH-induced secretion. Tetradecanoylphorbol Acetate 69-72 gonadotropin releasing hormone 1 Rattus norvegicus 346-350 7007026-4 1981 However, hormone secretion declined rapidly, reaching basal levels within 12 h. Both the calcium ionophore A23187 and the cocarcinogen phorbol myristate acetate were effective in releasing additional LH and FSH from cells made refractory to 30 nM GnRH, whereas higher doses of GnRH and 8-bromo-cAMP were minimally active. Tetradecanoylphorbol Acetate 135-160 gonadotropin releasing hormone 1 Rattus norvegicus 247-251 7007026-4 1981 However, hormone secretion declined rapidly, reaching basal levels within 12 h. Both the calcium ionophore A23187 and the cocarcinogen phorbol myristate acetate were effective in releasing additional LH and FSH from cells made refractory to 30 nM GnRH, whereas higher doses of GnRH and 8-bromo-cAMP were minimally active. Tetradecanoylphorbol Acetate 135-160 gonadotropin releasing hormone 1 Rattus norvegicus 277-281 3106083-3 1987 On the other hand, TPA was effective only at dosages greater than 10(-6)M. These results suggest that the stimulatory effect of LHRH on arachidonic acid release is coupled more tightly to a Ca2+-dependent rather than a protein kinase C-mediated pathway. Tetradecanoylphorbol Acetate 19-22 gonadotropin releasing hormone 1 Rattus norvegicus 128-132