PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 3489545-5 1986 Cytochalasins not only increased IL-2 release, but could substitute for phorbol myristic acetate (PMA) in supporting mitogen-signaled IL-2 production. Tetradecanoylphorbol Acetate 98-101 interleukin 2 Homo sapiens 134-138 3490935-6 1986 However, impaired IL-2 production could be partly restored by either (1) adding PMA to the PHA-stimulated culture, or (2) supplementing indomethacin (IM) from the initiation of the culture, or (3) depletion of adherent cells from PBMC. Tetradecanoylphorbol Acetate 80-83 interleukin 2 Homo sapiens 18-22 3023305-1 1986 Phytohemagglutinin (PHA) and a tumor-promoting phorbol ester, 12-O-tetradecanoylphorbol-13-acetate (TPA) act synergistically to induce interleukin 2 (IL2) mRNA in human lymphocytes in vitro. Tetradecanoylphorbol Acetate 62-98 interleukin 2 Homo sapiens 135-148 3023305-1 1986 Phytohemagglutinin (PHA) and a tumor-promoting phorbol ester, 12-O-tetradecanoylphorbol-13-acetate (TPA) act synergistically to induce interleukin 2 (IL2) mRNA in human lymphocytes in vitro. Tetradecanoylphorbol Acetate 62-98 interleukin 2 Homo sapiens 150-153 3023305-1 1986 Phytohemagglutinin (PHA) and a tumor-promoting phorbol ester, 12-O-tetradecanoylphorbol-13-acetate (TPA) act synergistically to induce interleukin 2 (IL2) mRNA in human lymphocytes in vitro. Tetradecanoylphorbol Acetate 100-103 interleukin 2 Homo sapiens 135-148 3023305-1 1986 Phytohemagglutinin (PHA) and a tumor-promoting phorbol ester, 12-O-tetradecanoylphorbol-13-acetate (TPA) act synergistically to induce interleukin 2 (IL2) mRNA in human lymphocytes in vitro. Tetradecanoylphorbol Acetate 100-103 interleukin 2 Homo sapiens 150-153 3023305-7 1986 Prostaglandin E2, which is known to elevate intracellular cAMP level, also inhibited IL2 mRNA induction in the lymphocytes stimulated with PHA and TPA. Tetradecanoylphorbol Acetate 147-150 interleukin 2 Homo sapiens 85-88 3522247-2 1986 The IL2 response induced by these mAb observed both in the presence and absence of phorbol myristate acetate was in the range of that obtained when Jurkat cells were stimulated with phytohemagglutinin or anti-T3 mAb (Leu 4). Tetradecanoylphorbol Acetate 83-108 interleukin 2 Homo sapiens 4-7 2941417-2 1986 IL-2 receptors on normal human T lymphocytes and the leukemic cell line, HUT102B2, are rapidly phosphorylated in vivo in response to the tumor-promoting phorbol ester, 12-O-tetradecanoylphorbol 13-acetate (TPA). Tetradecanoylphorbol Acetate 168-204 interleukin 2 Homo sapiens 0-4 2941417-2 1986 IL-2 receptors on normal human T lymphocytes and the leukemic cell line, HUT102B2, are rapidly phosphorylated in vivo in response to the tumor-promoting phorbol ester, 12-O-tetradecanoylphorbol 13-acetate (TPA). Tetradecanoylphorbol Acetate 206-209 interleukin 2 Homo sapiens 0-4 3011152-5 1986 Induction of Tac antigen, a putative interleukin 2 (IL 2) receptor, was observed in two cases after cultivation with PMA or with a novel lymphokine, adult T cell leukemia-derived factor (ADF). Tetradecanoylphorbol Acetate 117-120 interleukin 2 Homo sapiens 52-56 3007165-1 1986 The enhanced expression of interleukin 2 (IL2) receptors by 12-O-tetradecanoylphorbol 13-acetate (TPA) on sublines of an Epstein-Barr virus-immortalized human B17 B cell line (M. Steinitz et al., Immunobiology 1979. Tetradecanoylphorbol Acetate 98-101 interleukin 2 Homo sapiens 27-40 3083761-9 1986 The antigen-exogenous IL2-driven pathway of HILDA production by clones was bypassed by use of either PMA or calcium ionophore (CaI) alone or associated in the culture. Tetradecanoylphorbol Acetate 101-104 interleukin 2 Homo sapiens 22-25 3489679-3 1986 Addition of phorbol myristate acetate to the cultures resulted in enhancement of IL 2 production, but there was no significant promotion when indomethacin or human lymphoblastoid cell lines were added to the cultures. Tetradecanoylphorbol Acetate 12-37 interleukin 2 Homo sapiens 81-85 3001178-2 1986 Interleukin 2 (IL 2) production by these lines can be induced by phytohemagglutinin (PHA), T3 antibodies, or calcium ionophores, but only in combination with phorbol myristate acetate (PMA). Tetradecanoylphorbol Acetate 158-183 interleukin 2 Homo sapiens 0-13 3001178-2 1986 Interleukin 2 (IL 2) production by these lines can be induced by phytohemagglutinin (PHA), T3 antibodies, or calcium ionophores, but only in combination with phorbol myristate acetate (PMA). Tetradecanoylphorbol Acetate 158-183 interleukin 2 Homo sapiens 15-19 3001178-2 1986 Interleukin 2 (IL 2) production by these lines can be induced by phytohemagglutinin (PHA), T3 antibodies, or calcium ionophores, but only in combination with phorbol myristate acetate (PMA). Tetradecanoylphorbol Acetate 185-188 interleukin 2 Homo sapiens 0-13 3001178-2 1986 Interleukin 2 (IL 2) production by these lines can be induced by phytohemagglutinin (PHA), T3 antibodies, or calcium ionophores, but only in combination with phorbol myristate acetate (PMA). Tetradecanoylphorbol Acetate 185-188 interleukin 2 Homo sapiens 15-19 3082877-2 1986 The recombinant IL-2 receptor in these cells was rapidly phosphorylated in response to phorbol myristate acetate (PMA), but its phosphorylation could not be detected in the absence of PMA or upon addition of human IL-2. Tetradecanoylphorbol Acetate 87-112 interleukin 2 Homo sapiens 16-20 3082877-2 1986 The recombinant IL-2 receptor in these cells was rapidly phosphorylated in response to phorbol myristate acetate (PMA), but its phosphorylation could not be detected in the absence of PMA or upon addition of human IL-2. Tetradecanoylphorbol Acetate 114-117 interleukin 2 Homo sapiens 16-20 3082877-2 1986 The recombinant IL-2 receptor in these cells was rapidly phosphorylated in response to phorbol myristate acetate (PMA), but its phosphorylation could not be detected in the absence of PMA or upon addition of human IL-2. Tetradecanoylphorbol Acetate 184-187 interleukin 2 Homo sapiens 16-20 3086215-1 1986 Cultures of human blood mononuclear cells incubated with the calcium ionophore A 23 187 in the presence of the tumor promoter phorbol 12-myristate-13-acetate (PMA) produced 5-10 times more of the lymphokines interleukin 2 (IL 2) and interferon-gamma (IFN-gamma) than cultures which were stimulated with other combinations of inducing agents and PMA. Tetradecanoylphorbol Acetate 126-157 interleukin 2 Homo sapiens 208-221 3086215-1 1986 Cultures of human blood mononuclear cells incubated with the calcium ionophore A 23 187 in the presence of the tumor promoter phorbol 12-myristate-13-acetate (PMA) produced 5-10 times more of the lymphokines interleukin 2 (IL 2) and interferon-gamma (IFN-gamma) than cultures which were stimulated with other combinations of inducing agents and PMA. Tetradecanoylphorbol Acetate 126-157 interleukin 2 Homo sapiens 223-227 3086215-1 1986 Cultures of human blood mononuclear cells incubated with the calcium ionophore A 23 187 in the presence of the tumor promoter phorbol 12-myristate-13-acetate (PMA) produced 5-10 times more of the lymphokines interleukin 2 (IL 2) and interferon-gamma (IFN-gamma) than cultures which were stimulated with other combinations of inducing agents and PMA. Tetradecanoylphorbol Acetate 159-162 interleukin 2 Homo sapiens 208-221 3086215-1 1986 Cultures of human blood mononuclear cells incubated with the calcium ionophore A 23 187 in the presence of the tumor promoter phorbol 12-myristate-13-acetate (PMA) produced 5-10 times more of the lymphokines interleukin 2 (IL 2) and interferon-gamma (IFN-gamma) than cultures which were stimulated with other combinations of inducing agents and PMA. Tetradecanoylphorbol Acetate 159-162 interleukin 2 Homo sapiens 223-227 2419474-3 1986 The induction of IL-2 and IFN mRNAs required the synergistic action of PMA and either PHA or OKT3 mAb. Tetradecanoylphorbol Acetate 71-74 interleukin 2 Homo sapiens 17-29 3488373-5 1986 Two monoclonal antibodies (designated as KNT-1 and KNT-2) out of six established clones reacted with natural human IL-2 molecule obtained from cultured human peripheral blood lymphocytes (PBL) stimulated with PHA-P and TPA. Tetradecanoylphorbol Acetate 219-222 interleukin 2 Homo sapiens 115-119 3484763-3 1986 Maximal levels of IL 2 mRNA were reached 6 hr after induction of Jurkat cells with a combination of mitogen phytohemagglutinin (PHA) and phorbol ester (TPA). Tetradecanoylphorbol Acetate 152-155 interleukin 2 Homo sapiens 18-22 3484763-4 1986 Antibody 9.6, added during the first 4 hr after lymphocyte stimulation, markedly inhibited IL 2 mRNA accumulation induced by low but synergistic combination of PHA (5 micrograms/ml) and TPA (1.0 ng/ml). Tetradecanoylphorbol Acetate 186-189 interleukin 2 Homo sapiens 91-95 3484763-9 1986 These findings indicate that signals induced by antibody 9.6 regulate IL 2 production at a pre-translational level, are operative for an extended period of time overlapping with the early phase of IL 2 mRNA accumulation, suppress IL 2 gene expression induced by PHA as well as TPA, and that antibody 9.6 and CsA exert their inhibitory effect by distinct mechanism(s). Tetradecanoylphorbol Acetate 277-280 interleukin 2 Homo sapiens 70-74 3484763-9 1986 These findings indicate that signals induced by antibody 9.6 regulate IL 2 production at a pre-translational level, are operative for an extended period of time overlapping with the early phase of IL 2 mRNA accumulation, suppress IL 2 gene expression induced by PHA as well as TPA, and that antibody 9.6 and CsA exert their inhibitory effect by distinct mechanism(s). Tetradecanoylphorbol Acetate 277-280 interleukin 2 Homo sapiens 197-201 3484763-9 1986 These findings indicate that signals induced by antibody 9.6 regulate IL 2 production at a pre-translational level, are operative for an extended period of time overlapping with the early phase of IL 2 mRNA accumulation, suppress IL 2 gene expression induced by PHA as well as TPA, and that antibody 9.6 and CsA exert their inhibitory effect by distinct mechanism(s). Tetradecanoylphorbol Acetate 277-280 interleukin 2 Homo sapiens 197-201 3007165-1 1986 The enhanced expression of interleukin 2 (IL2) receptors by 12-O-tetradecanoylphorbol 13-acetate (TPA) on sublines of an Epstein-Barr virus-immortalized human B17 B cell line (M. Steinitz et al., Immunobiology 1979. Tetradecanoylphorbol Acetate 60-96 interleukin 2 Homo sapiens 27-40 3007165-1 1986 The enhanced expression of interleukin 2 (IL2) receptors by 12-O-tetradecanoylphorbol 13-acetate (TPA) on sublines of an Epstein-Barr virus-immortalized human B17 B cell line (M. Steinitz et al., Immunobiology 1979. Tetradecanoylphorbol Acetate 60-96 interleukin 2 Homo sapiens 42-45 3007165-1 1986 The enhanced expression of interleukin 2 (IL2) receptors by 12-O-tetradecanoylphorbol 13-acetate (TPA) on sublines of an Epstein-Barr virus-immortalized human B17 B cell line (M. Steinitz et al., Immunobiology 1979. Tetradecanoylphorbol Acetate 98-101 interleukin 2 Homo sapiens 42-45 3091996-2 1986 The IL 2 production could be greatly augmented by the addition of a phorbol ester, 12-O-tetradecanoyl-phorbol-13-acetate (TPA). Tetradecanoylphorbol Acetate 83-120 interleukin 2 Homo sapiens 4-8 3091996-2 1986 The IL 2 production could be greatly augmented by the addition of a phorbol ester, 12-O-tetradecanoyl-phorbol-13-acetate (TPA). Tetradecanoylphorbol Acetate 122-125 interleukin 2 Homo sapiens 4-8 3091996-6 1986 Remarkable IL 2 production was also induced by OKT3 when latex beads coated with rabbit anti-mouse IgG2a antibody and TPA were added to the culture. Tetradecanoylphorbol Acetate 118-121 interleukin 2 Homo sapiens 11-15 3007165-5 1986 IL2-binding studies revealed that TPA induced an increase in not only the number of IL2 receptors per cell but also the affinity of the receptors for IL2. Tetradecanoylphorbol Acetate 34-37 interleukin 2 Homo sapiens 0-3 3007165-5 1986 IL2-binding studies revealed that TPA induced an increase in not only the number of IL2 receptors per cell but also the affinity of the receptors for IL2. Tetradecanoylphorbol Acetate 34-37 interleukin 2 Homo sapiens 84-87 3007165-5 1986 IL2-binding studies revealed that TPA induced an increase in not only the number of IL2 receptors per cell but also the affinity of the receptors for IL2. Tetradecanoylphorbol Acetate 34-37 interleukin 2 Homo sapiens 84-87 3007165-6 1986 The number and affinity of IL2 receptors on the C76 subline treated with TPA appear to be similar to those of activated normal human peripheral T cells. Tetradecanoylphorbol Acetate 73-76 interleukin 2 Homo sapiens 27-30 2932270-10 1985 The addition of PHA or PHA plus TPA led to an increase in IL-2 production. Tetradecanoylphorbol Acetate 32-35 interleukin 2 Homo sapiens 58-62 3002691-4 1985 In addition, T cells cultured with VIT3 plus TPA but not with VIT3 or TPA alone express high levels of interleukin 2 (IL-2) receptors and transferrin receptors. Tetradecanoylphorbol Acetate 45-48 interleukin 2 Homo sapiens 103-116 3002691-4 1985 In addition, T cells cultured with VIT3 plus TPA but not with VIT3 or TPA alone express high levels of interleukin 2 (IL-2) receptors and transferrin receptors. Tetradecanoylphorbol Acetate 45-48 interleukin 2 Homo sapiens 118-122 3937226-6 1985 Phorbol myristic acetate (PMA) at non-mitogenic concentrations exerted an extremely strong synergistic effect on A23187-induced cell proliferation, which was, again, mediated via an IL-2-dependent pathway. Tetradecanoylphorbol Acetate 26-29 interleukin 2 Homo sapiens 182-186 3936721-2 1985 A tumor-promoting phorbol ester, 12-O-tetradecanoyl-phorbol 13-acetate (TPA), acted synergistically with a Ca2+ ionophore A23187 or phytohemagglutinin (PHA) to induce a high level of IL2 mRNA in lymphocytes, whereas each of them by itself could not induce the mRNA production. Tetradecanoylphorbol Acetate 33-70 interleukin 2 Homo sapiens 183-186 3936721-2 1985 A tumor-promoting phorbol ester, 12-O-tetradecanoyl-phorbol 13-acetate (TPA), acted synergistically with a Ca2+ ionophore A23187 or phytohemagglutinin (PHA) to induce a high level of IL2 mRNA in lymphocytes, whereas each of them by itself could not induce the mRNA production. Tetradecanoylphorbol Acetate 72-75 interleukin 2 Homo sapiens 183-186 3936721-3 1985 In two-step culture experiments the lymphocytes pulse-incubated with TPA for 1 h (the first culture) could efficiently initiate IL2 mRNA production by subsequent culture with A23187 or PHA (the second culture). Tetradecanoylphorbol Acetate 69-72 interleukin 2 Homo sapiens 128-131 3936721-7 1985 These results show that mobilization of Ca2+ and the calmodulin-dependent regulatory system appear to work synergistically with TPA which probably activates protein kinase C in the pathway to IL2 gene expression. Tetradecanoylphorbol Acetate 128-131 interleukin 2 Homo sapiens 192-195 3937226-8 1985 Combination of PMA and A23187 resulted in considerable IL-2 production. Tetradecanoylphorbol Acetate 15-18 interleukin 2 Homo sapiens 55-59 3930891-5 1985 IL 2 had the ability to restore lytic activity to PMA-treated cells but did not induce IFN gamma production. Tetradecanoylphorbol Acetate 50-53 interleukin 2 Homo sapiens 0-4 3161562-3 1985 The receptors for IL 2, which were initially absent from the cell surface, were induced on high percentages of the ALL cells after the in vitro exposure to the lectin phytohemagglutinin or the phorbol ester 12-O-tetradecanoylphorbol-13-acetate in six patients, suggesting that the cells had become sensitive to IL 2. Tetradecanoylphorbol Acetate 207-243 interleukin 2 Homo sapiens 18-22 2992637-3 1985 By contrast, after stimulation with phytohemagglutinin (PHA) or with PHA plus 12-O-tetradecanoylphorbol-13-acetate, the production of IL 2 and IFN-gamma by B-CLL T lymphocytes was similar to that of normal T lymphocytes, irrespective of the reversed T lymphocyte subset distribution (OKT4/OKT8 ratio) observed in B-CLL. Tetradecanoylphorbol Acetate 78-114 interleukin 2 Homo sapiens 134-138 3161562-3 1985 The receptors for IL 2, which were initially absent from the cell surface, were induced on high percentages of the ALL cells after the in vitro exposure to the lectin phytohemagglutinin or the phorbol ester 12-O-tetradecanoylphorbol-13-acetate in six patients, suggesting that the cells had become sensitive to IL 2. Tetradecanoylphorbol Acetate 207-243 interleukin 2 Homo sapiens 311-315 2992482-6 1985 Additionally, TPA and TCD both induced a high density of cell surface receptors for interleukin 2 (IL2) and transferrin, but not synthesis or production of IL2. Tetradecanoylphorbol Acetate 14-17 interleukin 2 Homo sapiens 84-97 3875683-8 1985 This mutant, termed JA3 (surface phenotype: T11+, T3+, 3A1+, T4-, T8-, DR-, Tac-, 4F2+, T44+) produced large amounts of IL-2 upon stimulation with PHA, anti-T3, or anticlonotypic mAb in conjunction with phorbol myristate acetate (or adherent cells). Tetradecanoylphorbol Acetate 203-228 interleukin 2 Homo sapiens 120-124 3926889-8 1985 Treatment of cells with subinducing doses of Con A or phorbol myristate acetate increased IFN-gamma induction by exogenous IL 2. Tetradecanoylphorbol Acetate 54-79 interleukin 2 Homo sapiens 123-127 2410377-2 1985 JA3 had been selected because of its ability to release large amounts of IL-2 following stimulation with phytohemagglutinin (PHA) or anti-T3 antibody in the presence of phorbolmyristate acetate (PMA). Tetradecanoylphorbol Acetate 169-193 interleukin 2 Homo sapiens 73-77 2992482-6 1985 Additionally, TPA and TCD both induced a high density of cell surface receptors for interleukin 2 (IL2) and transferrin, but not synthesis or production of IL2. Tetradecanoylphorbol Acetate 14-17 interleukin 2 Homo sapiens 99-102 3874078-7 1985 Furthermore, two stimuli (IL 1 and phorbol myristate acetate), which are able to replace monocytes at the level of IL2 production, also induce responsiveness to IL2 under accessory cell-dependent conditions. Tetradecanoylphorbol Acetate 35-60 interleukin 2 Homo sapiens 161-164 3159820-8 1985 T cell activation with mAb 9.3 and TPA was associated with increases in interleukin 2(IL-2) receptor expression and IL-2 secretion. Tetradecanoylphorbol Acetate 35-38 interleukin 2 Homo sapiens 86-90 3097429-1 1986 Absolute capacity for IL2 production by human peripheral blood mononuclear cells (PBMC) was studied using 12-O-tetradecanoylphorbol 13-acetate (TPA) and calcium ionophore A23187, which act synergistically, to induce lymphokine synthesis. Tetradecanoylphorbol Acetate 106-142 interleukin 2 Homo sapiens 22-25 3097429-1 1986 Absolute capacity for IL2 production by human peripheral blood mononuclear cells (PBMC) was studied using 12-O-tetradecanoylphorbol 13-acetate (TPA) and calcium ionophore A23187, which act synergistically, to induce lymphokine synthesis. Tetradecanoylphorbol Acetate 144-147 interleukin 2 Homo sapiens 22-25 3097429-2 1986 Culture parameters were optimized for the TPA/A23187 stimulation such that maximal IL2 titers were produced with a high degree of reproducibility. Tetradecanoylphorbol Acetate 42-45 interleukin 2 Homo sapiens 83-86 3097429-3 1986 Thus, using a synthetic medium, TPA/A23187 at 20/50ng/ml respectively, and a cell concentration of 2.5 X 10(6)/ml, IL2 titers in the cultures increased linearly over a period of 96h, reaching values at least 15-fold higher than with lectin stimulation. Tetradecanoylphorbol Acetate 32-35 interleukin 2 Homo sapiens 115-118 3876332-2 1985 A remarkable increase of IL2 mRNA was induced by stimulation with phytohemagglutinin (PHA) in the presence of 12-O-tetradecanoyl-phorbol-13-acetate (TPA). Tetradecanoylphorbol Acetate 110-147 interleukin 2 Homo sapiens 25-28 3876332-2 1985 A remarkable increase of IL2 mRNA was induced by stimulation with phytohemagglutinin (PHA) in the presence of 12-O-tetradecanoyl-phorbol-13-acetate (TPA). Tetradecanoylphorbol Acetate 149-152 interleukin 2 Homo sapiens 25-28 3876332-5 1985 Two-step culture experiments showed that prior exposure of the lymphocytes to TPA for 1 h at 37 degrees C resulted in a remarkable increase of IL2 mRNA on subsequent stimulation with PHA. Tetradecanoylphorbol Acetate 78-81 interleukin 2 Homo sapiens 143-146 3876332-6 1985 This suggests that TPA induces certain changes in the biochemical pathway of signal transduction so that the cells can be triggered to express IL2 gene by subsequent stimulation with mitogen. Tetradecanoylphorbol Acetate 19-22 interleukin 2 Homo sapiens 143-146 3159820-8 1985 T cell activation with mAb 9.3 and TPA was associated with increases in interleukin 2(IL-2) receptor expression and IL-2 secretion. Tetradecanoylphorbol Acetate 35-38 interleukin 2 Homo sapiens 116-120 3921610-4 1985 The Ca++ ionophore ionomycin and TPA, used in conjunction, mimicked the effect of specific alloantigen on these T cell clones, i.e., they induced the secretion of IFN-gamma in all clones and the secretion of IL 2 in the T4+ clone. Tetradecanoylphorbol Acetate 33-36 interleukin 2 Homo sapiens 208-212 3921610-1 1985 A previous study indicated that Ca++ ionophores in conjunction with the phorbol ester 12-O-tetradecanoylphorbol 13-acetate (TPA) could induce normal T lymphocytes to express receptors for the T cell growth factor, interleukin 2 (IL 2), to secrete IL 2, and to proliferate (1). Tetradecanoylphorbol Acetate 124-127 interleukin 2 Homo sapiens 192-212 3921610-1 1985 A previous study indicated that Ca++ ionophores in conjunction with the phorbol ester 12-O-tetradecanoylphorbol 13-acetate (TPA) could induce normal T lymphocytes to express receptors for the T cell growth factor, interleukin 2 (IL 2), to secrete IL 2, and to proliferate (1). Tetradecanoylphorbol Acetate 124-127 interleukin 2 Homo sapiens 214-227 3921610-1 1985 A previous study indicated that Ca++ ionophores in conjunction with the phorbol ester 12-O-tetradecanoylphorbol 13-acetate (TPA) could induce normal T lymphocytes to express receptors for the T cell growth factor, interleukin 2 (IL 2), to secrete IL 2, and to proliferate (1). Tetradecanoylphorbol Acetate 124-127 interleukin 2 Homo sapiens 229-233 3921610-1 1985 A previous study indicated that Ca++ ionophores in conjunction with the phorbol ester 12-O-tetradecanoylphorbol 13-acetate (TPA) could induce normal T lymphocytes to express receptors for the T cell growth factor, interleukin 2 (IL 2), to secrete IL 2, and to proliferate (1). Tetradecanoylphorbol Acetate 124-127 interleukin 2 Homo sapiens 247-251 3921610-6 1985 Increased sensitivity to exogenous IL 2 for some T cell clones was also observed after either alloantigen or ionomycin and TPA treatment; this could be correlated with an increase in the expression of IL 2 receptors 6 hr after a pulse with ionomycin and TPA. Tetradecanoylphorbol Acetate 123-126 interleukin 2 Homo sapiens 35-39 3921610-6 1985 Increased sensitivity to exogenous IL 2 for some T cell clones was also observed after either alloantigen or ionomycin and TPA treatment; this could be correlated with an increase in the expression of IL 2 receptors 6 hr after a pulse with ionomycin and TPA. Tetradecanoylphorbol Acetate 254-257 interleukin 2 Homo sapiens 35-39 3921610-6 1985 Increased sensitivity to exogenous IL 2 for some T cell clones was also observed after either alloantigen or ionomycin and TPA treatment; this could be correlated with an increase in the expression of IL 2 receptors 6 hr after a pulse with ionomycin and TPA. Tetradecanoylphorbol Acetate 254-257 interleukin 2 Homo sapiens 201-205 2580014-0 1985 Comparison of the expression of IL 2 receptors by human T and B cells: induction by the polyclonal mitogens, phorbol myristate acetate, and anti-mu antibody. Tetradecanoylphorbol Acetate 109-134 interleukin 2 Homo sapiens 32-36 2985401-1 1985 The expression of the interleukin 2 receptor (Tac) on normal B cells and Epstein-Barr virus-transformed lymphoblastoid B cell lines is induced by the tumor promoter 12-O-tetradecanoyl-phorbol-13-acetate (TPA). Tetradecanoylphorbol Acetate 204-207 interleukin 2 Homo sapiens 22-35 2985401-3 1985 It is functionally active so that TPA-induced B cells respond to interleukin 2 by increased DNA synthesis. Tetradecanoylphorbol Acetate 34-37 interleukin 2 Homo sapiens 65-78 3930011-1 1985 Tumor promoting phorbol myristate acetate (PMA) induce to enhance the expression of IL2 Receptor and to decrease the antigen receptor expression on the cell surface. Tetradecanoylphorbol Acetate 16-41 interleukin 2 Homo sapiens 84-87 3920341-10 1985 TPA induced a low level of IL-2 receptor expression in monocyte-depleted T cells, without inducing IL-2 secretion. Tetradecanoylphorbol Acetate 0-3 interleukin 2 Homo sapiens 27-31 3920341-11 1985 Anti-T3 plus TPA induced a marked enhancement in both quantity and intensity of IL-2 receptor expression. Tetradecanoylphorbol Acetate 13-16 interleukin 2 Homo sapiens 80-84 2981919-5 1985 Furthermore, in cultures stimulated by a combination of PHA plus TPA, 9.6 did not inhibit the acquisition of IL 2 receptors but inhibited proliferation and IL 2 production. Tetradecanoylphorbol Acetate 65-68 interleukin 2 Homo sapiens 156-160 3918872-1 1985 The tumor promoter 12-O-tetradecanoylphorbol 13-acetate induces the expression of the interleukin 2 receptor and the disappearance of the T3 complex in a T cell hybridoma, T cell clones, thymic and peripheral T cells and T cell tumors. Tetradecanoylphorbol Acetate 19-55 interleukin 2 Homo sapiens 86-99 2981271-10 1985 Thymocytes activated with TPA and Con A were more resistant to the inhibitory effects of Dex on the expression of IL 2 receptors than thymocytes activated with Con A alone. Tetradecanoylphorbol Acetate 26-29 interleukin 2 Homo sapiens 114-118 3930011-1 1985 Tumor promoting phorbol myristate acetate (PMA) induce to enhance the expression of IL2 Receptor and to decrease the antigen receptor expression on the cell surface. Tetradecanoylphorbol Acetate 43-46 interleukin 2 Homo sapiens 84-87 6237693-5 1984 Stimulation with PMA, however, resulted in significant IL-2 production. Tetradecanoylphorbol Acetate 17-20 interleukin 2 Homo sapiens 55-59 3874078-7 1985 Furthermore, two stimuli (IL 1 and phorbol myristate acetate), which are able to replace monocytes at the level of IL2 production, also induce responsiveness to IL2 under accessory cell-dependent conditions. Tetradecanoylphorbol Acetate 35-60 interleukin 2 Homo sapiens 115-118 6439651-8 1984 Washing of these T-PLL cells to remove TPA resulted in the induction of proliferation upon subsequent culture in the presence of IL-2 or in medium only. Tetradecanoylphorbol Acetate 39-42 interleukin 2 Homo sapiens 129-133 6237101-2 1984 Receptor-negative human T leukemic cell lines were induced to express the IL 2 receptor after incubation with 12-O-tetradecanoyl phorbol 13-acetate. Tetradecanoylphorbol Acetate 110-147 interleukin 2 Homo sapiens 74-78 6237101-5 1984 The precursor of the IL 2 receptor isolated from tunicamycin-treated HUT102B2 or 12-O-tetradecanoyl phorbol 13-acetate-induced CCRF-CEM cells had the same Mr and isoelectric point. Tetradecanoylphorbol Acetate 81-118 interleukin 2 Homo sapiens 21-25 6237101-6 1984 Incubation of cells with 12-O-tetradecanoyl phorbol 13-acetate also induced the rapid phosphorylation of serine and threonine residues on the IL 2 receptor from HUT102B2 cells and activated peripheral blood lymphocytes. Tetradecanoylphorbol Acetate 25-62 interleukin 2 Homo sapiens 142-146 6331899-10 1984 TPA was also tested for its ability to "maintain" activated T-cell blasts in a standard assay for interleukin 2 (IL-2). Tetradecanoylphorbol Acetate 0-3 interleukin 2 Homo sapiens 98-111 6331899-10 1984 TPA was also tested for its ability to "maintain" activated T-cell blasts in a standard assay for interleukin 2 (IL-2). Tetradecanoylphorbol Acetate 0-3 interleukin 2 Homo sapiens 113-117 6611555-9 1984 IL-2 production by the patient"s phytohemagglutin-stimulated PBMC was severely deficient but was corrected by the addition of phorbol 12-myristate 13-acetate, suggesting a defective response to IL-1. Tetradecanoylphorbol Acetate 126-157 interleukin 2 Homo sapiens 0-4 6332315-5 1984 These studies were performed with a cloned human leukemic T-cell line (Jurkat, subclone 32), which can be induced with phytohemagglutinin and phorbol 12-myristate 13-acetate to produce large amounts of TCGF. Tetradecanoylphorbol Acetate 142-173 interleukin 2 Homo sapiens 202-206 6609189-1 1984 TPA alone did not induce the production of IL 2 in human tonsillar lymphocytes but enhanced the PHA-induced IL 2 production by seven-fold. Tetradecanoylphorbol Acetate 0-3 interleukin 2 Homo sapiens 108-112 6234599-2 1984 Incubation of Jurkat with phytohemagglutinin (PHA) resulted in the production of interleukin 2, which was markedly increased by the addition of phorbol 12-myristate 13-acetate (PMA). Tetradecanoylphorbol Acetate 144-175 interleukin 2 Homo sapiens 81-94 6234599-2 1984 Incubation of Jurkat with phytohemagglutinin (PHA) resulted in the production of interleukin 2, which was markedly increased by the addition of phorbol 12-myristate 13-acetate (PMA). Tetradecanoylphorbol Acetate 177-180 interleukin 2 Homo sapiens 81-94 6335665-3 1984 A human leukemic T-cell line (Jurkat) can be induced with the lectin phytohemagglutinin and the phorbol ester tetradecanoylphorbolacetate (TPA) to produce T-cell growth factor. Tetradecanoylphorbol Acetate 139-142 interleukin 2 Homo sapiens 155-175 6335665-7 1984 Northern blot experiments with cloned TCGF DNA as a probe showed that TCGF mRNA was induced rapidly in cells treated with TPA and phytohemagglutinin, and this induction was augmented by interleukin 1. Tetradecanoylphorbol Acetate 122-125 interleukin 2 Homo sapiens 38-42 6335665-7 1984 Northern blot experiments with cloned TCGF DNA as a probe showed that TCGF mRNA was induced rapidly in cells treated with TPA and phytohemagglutinin, and this induction was augmented by interleukin 1. Tetradecanoylphorbol Acetate 122-125 interleukin 2 Homo sapiens 70-74 6335665-9 1984 Nuclear transcription experiments suggested that the TCGF gene was more actively transcribed in cells treated with TPA and phytohemagglutinin than in cells treated with phytohemagglutinin alone. Tetradecanoylphorbol Acetate 115-118 interleukin 2 Homo sapiens 53-57 6335665-10 1984 The transcription of the TCGF gene was further increased when interleukin 1 was included along with TPA and phytohemagglutinin. Tetradecanoylphorbol Acetate 100-103 interleukin 2 Homo sapiens 25-29 6609189-2 1984 That the effect of TPA was due to an increase in IL 2 mRNA was demonstrated by examining the amount of IL 2 mRNA translatable in Xenopus laevis oocytes, and by Northern blotting analysis using IL 2 cDNA as a probe. Tetradecanoylphorbol Acetate 19-22 interleukin 2 Homo sapiens 49-53 6609189-2 1984 That the effect of TPA was due to an increase in IL 2 mRNA was demonstrated by examining the amount of IL 2 mRNA translatable in Xenopus laevis oocytes, and by Northern blotting analysis using IL 2 cDNA as a probe. Tetradecanoylphorbol Acetate 19-22 interleukin 2 Homo sapiens 103-107 6609189-2 1984 That the effect of TPA was due to an increase in IL 2 mRNA was demonstrated by examining the amount of IL 2 mRNA translatable in Xenopus laevis oocytes, and by Northern blotting analysis using IL 2 cDNA as a probe. Tetradecanoylphorbol Acetate 19-22 interleukin 2 Homo sapiens 103-107 6609189-3 1984 In these ways, it was shown that TPA alone did not induce any significant IL 2 mRNA synthesis, but when added together with PHA it increased the level of IL 2 mRNA by at least 10-fold, as compared with that induced by PHA alone. Tetradecanoylphorbol Acetate 33-36 interleukin 2 Homo sapiens 154-158 6417283-4 1983 Mezerein (MZN), a compound structurally related to TPA, was found to be similar to TPA in enhancing IFN-gamma and IL-2 levels. Tetradecanoylphorbol Acetate 83-86 interleukin 2 Homo sapiens 114-118 6609121-8 1984 One clone expressing IL-2 receptors could be induced to produce human IL-2 by simultaneously stimulating with PHA and phorbol myristate acetate (PMA). Tetradecanoylphorbol Acetate 118-143 interleukin 2 Homo sapiens 21-25 6609121-8 1984 One clone expressing IL-2 receptors could be induced to produce human IL-2 by simultaneously stimulating with PHA and phorbol myristate acetate (PMA). Tetradecanoylphorbol Acetate 118-143 interleukin 2 Homo sapiens 70-74 6609121-8 1984 One clone expressing IL-2 receptors could be induced to produce human IL-2 by simultaneously stimulating with PHA and phorbol myristate acetate (PMA). Tetradecanoylphorbol Acetate 145-148 interleukin 2 Homo sapiens 21-25 6609121-8 1984 One clone expressing IL-2 receptors could be induced to produce human IL-2 by simultaneously stimulating with PHA and phorbol myristate acetate (PMA). Tetradecanoylphorbol Acetate 145-148 interleukin 2 Homo sapiens 70-74 6607173-4 1983 In the cultures containing a sufficient amount of exogenous T cell growth factor (TCGF), the enhancement of T lymphocyte colony formation by TPA was not observed. Tetradecanoylphorbol Acetate 141-144 interleukin 2 Homo sapiens 60-80 6607173-4 1983 In the cultures containing a sufficient amount of exogenous T cell growth factor (TCGF), the enhancement of T lymphocyte colony formation by TPA was not observed. Tetradecanoylphorbol Acetate 141-144 interleukin 2 Homo sapiens 82-86 6607173-5 1983 TPA enhanced TCGF production by peripheral lymphocytes stimulated with PHA. Tetradecanoylphorbol Acetate 0-3 interleukin 2 Homo sapiens 13-17 6607173-7 1983 These findings suggest that TPA enhanced T lymphocyte colony formation by stimulating endogenous TCGF production. Tetradecanoylphorbol Acetate 28-31 interleukin 2 Homo sapiens 97-101 6409425-1 1983 Previous studies showed that the tumor-promoting phorbol ester 12-O-tetradecanoylphorbol 13-acetate (TPA) and several structurally related tumor-promoting compounds stimulate lymphocytes to produce immune interferon (IFN-gamma) and interleukin 2 (IL-2). Tetradecanoylphorbol Acetate 63-99 interleukin 2 Homo sapiens 232-245 6409425-1 1983 Previous studies showed that the tumor-promoting phorbol ester 12-O-tetradecanoylphorbol 13-acetate (TPA) and several structurally related tumor-promoting compounds stimulate lymphocytes to produce immune interferon (IFN-gamma) and interleukin 2 (IL-2). Tetradecanoylphorbol Acetate 63-99 interleukin 2 Homo sapiens 247-251 6409425-1 1983 Previous studies showed that the tumor-promoting phorbol ester 12-O-tetradecanoylphorbol 13-acetate (TPA) and several structurally related tumor-promoting compounds stimulate lymphocytes to produce immune interferon (IFN-gamma) and interleukin 2 (IL-2). Tetradecanoylphorbol Acetate 101-104 interleukin 2 Homo sapiens 232-245 6409425-1 1983 Previous studies showed that the tumor-promoting phorbol ester 12-O-tetradecanoylphorbol 13-acetate (TPA) and several structurally related tumor-promoting compounds stimulate lymphocytes to produce immune interferon (IFN-gamma) and interleukin 2 (IL-2). Tetradecanoylphorbol Acetate 101-104 interleukin 2 Homo sapiens 247-251 6602780-7 1983 However, they could be induced to produce IL-2 activity by stimulation with TPA or TPA plus PHA. Tetradecanoylphorbol Acetate 76-79 interleukin 2 Homo sapiens 42-46 6602780-7 1983 However, they could be induced to produce IL-2 activity by stimulation with TPA or TPA plus PHA. Tetradecanoylphorbol Acetate 83-86 interleukin 2 Homo sapiens 42-46 6602780-8 1983 Irradiation of the proliferating T-CLL cells prior to incubation with TPA or TPA plus PHA resulted in a 9-fold increase in IL-2 activity, suggesting that the proliferating T-CLL cells were able to consume the IL-2 they produced. Tetradecanoylphorbol Acetate 70-73 interleukin 2 Homo sapiens 123-127 6602780-8 1983 Irradiation of the proliferating T-CLL cells prior to incubation with TPA or TPA plus PHA resulted in a 9-fold increase in IL-2 activity, suggesting that the proliferating T-CLL cells were able to consume the IL-2 they produced. Tetradecanoylphorbol Acetate 70-73 interleukin 2 Homo sapiens 209-213 6602780-8 1983 Irradiation of the proliferating T-CLL cells prior to incubation with TPA or TPA plus PHA resulted in a 9-fold increase in IL-2 activity, suggesting that the proliferating T-CLL cells were able to consume the IL-2 they produced. Tetradecanoylphorbol Acetate 77-80 interleukin 2 Homo sapiens 123-127 6602780-8 1983 Irradiation of the proliferating T-CLL cells prior to incubation with TPA or TPA plus PHA resulted in a 9-fold increase in IL-2 activity, suggesting that the proliferating T-CLL cells were able to consume the IL-2 they produced. Tetradecanoylphorbol Acetate 77-80 interleukin 2 Homo sapiens 209-213 6306584-1 1983 A recombinant plasmid containing human interleukin 2 (IL2) cDNA was identified in a cDNA library constructed from mRNA derived from PHA-TPA induced splenocytes. Tetradecanoylphorbol Acetate 136-139 interleukin 2 Homo sapiens 39-52 6306584-1 1983 A recombinant plasmid containing human interleukin 2 (IL2) cDNA was identified in a cDNA library constructed from mRNA derived from PHA-TPA induced splenocytes. Tetradecanoylphorbol Acetate 136-139 interleukin 2 Homo sapiens 54-57 6602179-9 1983 When incubated simultaneously with TPA and T cell growth factor (IL 2), all lines responded with greater DNA synthesis than when incubated with IL 2 or TPA alone, thus demonstrating a synergistic action between TPA and IL 2. Tetradecanoylphorbol Acetate 152-155 interleukin 2 Homo sapiens 43-63 6602179-9 1983 When incubated simultaneously with TPA and T cell growth factor (IL 2), all lines responded with greater DNA synthesis than when incubated with IL 2 or TPA alone, thus demonstrating a synergistic action between TPA and IL 2. Tetradecanoylphorbol Acetate 152-155 interleukin 2 Homo sapiens 65-69 6602179-9 1983 When incubated simultaneously with TPA and T cell growth factor (IL 2), all lines responded with greater DNA synthesis than when incubated with IL 2 or TPA alone, thus demonstrating a synergistic action between TPA and IL 2. Tetradecanoylphorbol Acetate 152-155 interleukin 2 Homo sapiens 43-63 6602179-9 1983 When incubated simultaneously with TPA and T cell growth factor (IL 2), all lines responded with greater DNA synthesis than when incubated with IL 2 or TPA alone, thus demonstrating a synergistic action between TPA and IL 2. Tetradecanoylphorbol Acetate 152-155 interleukin 2 Homo sapiens 65-69 6602934-1 1983 Poly(A)-positive mRNA extracted from tonsillar mononuclear cells stimulated with phytohemagglutinin-M and 12-o-tetradecanoyl phorbol 13-acetate was successfully translated into biologically active interleukin 2 (IL-2) in Xenopus laevis oocytes, and secreted into the incubation medium. Tetradecanoylphorbol Acetate 106-143 interleukin 2 Homo sapiens 197-210 33114591-4 2020 We show that venom treatment had a significant immunosuppressive effect, inhibiting the secretion of interleukin (IL)-2 and tumor necrosis factor (TNF) from purified human T cells by 90% or greater following stimulation with mitogen (phorbol 12-myristate 13-acetate and ionomycin) or via cluster of differentiation (CD) receptors, CD3/CD28. Tetradecanoylphorbol Acetate 234-265 interleukin 2 Homo sapiens 101-119 6778951-5 1980 Addition of the fatty acid derivative phorbol myristate acetate to mitogen-stimulated cultures increased Jurkat-FHCRC IL-2 production to concentrations > 400 U/ml. Tetradecanoylphorbol Acetate 38-63 interleukin 2 Homo sapiens 118-122 33374788-5 2020 It also inhibited IL-2 mRNA expression and NF-kappaB signaling mediated by phorbol 12-myristate 13-acetate, and phytohemagglutinin. Tetradecanoylphorbol Acetate 75-106 interleukin 2 Homo sapiens 18-22 6980126-6 1982 Stimulation of the IL2-producing JMN or JHAN variants with PHA, the phorbol diester 12-0-tetradecanoyl-phorbol-13-acetate (TPA), or both PHA and TPA together, resulted in an apparent increase of IL2 activity in the culture supernatant when assayed by a short-term tritiated thymidine incorporation test. Tetradecanoylphorbol Acetate 123-126 interleukin 2 Homo sapiens 19-22 6980126-6 1982 Stimulation of the IL2-producing JMN or JHAN variants with PHA, the phorbol diester 12-0-tetradecanoyl-phorbol-13-acetate (TPA), or both PHA and TPA together, resulted in an apparent increase of IL2 activity in the culture supernatant when assayed by a short-term tritiated thymidine incorporation test. Tetradecanoylphorbol Acetate 145-148 interleukin 2 Homo sapiens 19-22 6980126-10 1982 Addition of TPA, but not of PHA, to lectin and TPA-free JMN IL2 resulted in a decreased ability of such supernatants to support clonal T cell growth, suggesting that TPA had a growth-inhibiting effect. Tetradecanoylphorbol Acetate 12-15 interleukin 2 Homo sapiens 60-63 6980126-10 1982 Addition of TPA, but not of PHA, to lectin and TPA-free JMN IL2 resulted in a decreased ability of such supernatants to support clonal T cell growth, suggesting that TPA had a growth-inhibiting effect. Tetradecanoylphorbol Acetate 47-50 interleukin 2 Homo sapiens 60-63 6980126-10 1982 Addition of TPA, but not of PHA, to lectin and TPA-free JMN IL2 resulted in a decreased ability of such supernatants to support clonal T cell growth, suggesting that TPA had a growth-inhibiting effect. Tetradecanoylphorbol Acetate 47-50 interleukin 2 Homo sapiens 60-63 6980181-0 1982 The effect of phorbol-myristate acetate and concanavalin A on the growth of interleukin-2-dependent T-cell lines. Tetradecanoylphorbol Acetate 14-39 interleukin 2 Homo sapiens 76-89 6977565-2 1982 Human interleukin 2 (IL 2) was produced under serum-free conditions by stimulating mononuclear cells with concanavalin A (Con A) in the presence of phorbol myristate acetate (PMA) and hydroxyurea. Tetradecanoylphorbol Acetate 148-173 interleukin 2 Homo sapiens 6-19 6977565-2 1982 Human interleukin 2 (IL 2) was produced under serum-free conditions by stimulating mononuclear cells with concanavalin A (Con A) in the presence of phorbol myristate acetate (PMA) and hydroxyurea. Tetradecanoylphorbol Acetate 148-173 interleukin 2 Homo sapiens 21-25 6977565-2 1982 Human interleukin 2 (IL 2) was produced under serum-free conditions by stimulating mononuclear cells with concanavalin A (Con A) in the presence of phorbol myristate acetate (PMA) and hydroxyurea. Tetradecanoylphorbol Acetate 175-178 interleukin 2 Homo sapiens 6-19 6977565-2 1982 Human interleukin 2 (IL 2) was produced under serum-free conditions by stimulating mononuclear cells with concanavalin A (Con A) in the presence of phorbol myristate acetate (PMA) and hydroxyurea. Tetradecanoylphorbol Acetate 175-178 interleukin 2 Homo sapiens 21-25 32521784-3 2020 The artificial in vitro activation of CD4+ T lymphocytes by a combination of 12-O-tetradecanoylphorbol-13-acetate and ionomycin, the so-called T/I model, led to an inducible production of cytokines, such as interferon-gamma, tumor necrosis factor-alpha, and interleukin-2. Tetradecanoylphorbol Acetate 77-113 interleukin 2 Homo sapiens 258-271 30908093-3 2020 Phenylethanoids, acteoside (5) and isoacteoside (6) showed significant inhibitory to IL-2 secretion of with respect to phorbol myristate acetate and anti-CD28 monoclonal antibody co-stimulated activation of human peripheral blood T cells. Tetradecanoylphorbol Acetate 119-144 interleukin 2 Homo sapiens 85-89 32986722-7 2020 Stimulation with PMA restored signaling and (with ionomycin) IL-2 production. Tetradecanoylphorbol Acetate 17-20 interleukin 2 Homo sapiens 61-65 32189398-5 2020 In GC patients, iNKT cells, expanded in vitro with alpha-Galactosyl Ceramide and stimulated with PMA and Ionomycin, produced higher levels of IL-2 and TGF-beta, while their capacity to degranulate remained preserved. Tetradecanoylphorbol Acetate 97-100 interleukin 2 Homo sapiens 142-146 29933063-7 2018 We show that cells encapsulated in polymers of cellulose sulphate are able to release cytokines such as interleukin-2 (IL-2) in a stimulated fashion e.g. using phorbol 12-myristate 13-acetate (PMA) plus ionomycin. Tetradecanoylphorbol Acetate 160-191 interleukin 2 Homo sapiens 104-117 31649684-7 2019 Ex vivo examination of CD38+HLA-DR+CD8+ T cells indicated that this subset of cells displayed stronger secretion of IFN-gamma and IL-2 before and after a 6-h stimulation with phorbol 12-myristate 13-acetate (PMA) and ionomycin (ION) relative to healthy CD38+HLA-DR+CD8+ T cells, indicating the functional feasibility of CD38+HLA-DR+CD8+ T cells. Tetradecanoylphorbol Acetate 175-206 interleukin 2 Homo sapiens 130-134 31020588-2 2019 Preincubation of Jurkat cells with Ergoferon increased IL-2 secretion by these cells after stimulation with phorbol 12-myristate 13-acetate/ionomycine in comparison with the placebo group. Tetradecanoylphorbol Acetate 108-139 interleukin 2 Homo sapiens 55-59 29933063-7 2018 We show that cells encapsulated in polymers of cellulose sulphate are able to release cytokines such as interleukin-2 (IL-2) in a stimulated fashion e.g. using phorbol 12-myristate 13-acetate (PMA) plus ionomycin. Tetradecanoylphorbol Acetate 160-191 interleukin 2 Homo sapiens 119-123 29933063-7 2018 We show that cells encapsulated in polymers of cellulose sulphate are able to release cytokines such as interleukin-2 (IL-2) in a stimulated fashion e.g. using phorbol 12-myristate 13-acetate (PMA) plus ionomycin. Tetradecanoylphorbol Acetate 193-196 interleukin 2 Homo sapiens 104-117 29933063-7 2018 We show that cells encapsulated in polymers of cellulose sulphate are able to release cytokines such as interleukin-2 (IL-2) in a stimulated fashion e.g. using phorbol 12-myristate 13-acetate (PMA) plus ionomycin. Tetradecanoylphorbol Acetate 193-196 interleukin 2 Homo sapiens 119-123 29198314-13 2017 When activation of SMG-T cells occurred in SMG, the T cells produced less IL-2 than control T cell cultures upon incubation with PMA and ionomycin. Tetradecanoylphorbol Acetate 129-132 interleukin 2 Homo sapiens 74-78 29275177-5 2018 Phytohaemagglutinin and phorbol 12-myristate 13-acetate induced interleukin (IL)-2 production and activation of NF-kappaB signaling pathways, which were both inhibited by DES. Tetradecanoylphorbol Acetate 24-55 interleukin 2 Homo sapiens 64-82 29198314-10 2017 Following 72 h culture of T cells in SMG (SMG-T) or control static (Static-T) conditions, IL-2 production by the T cells was reduced in SMG-T cells compared to Static-T cells upon stimulation by phorbol 12-myristate 13-acetate (PMA) and ionomycin. Tetradecanoylphorbol Acetate 195-226 interleukin 2 Homo sapiens 90-94 29198314-10 2017 Following 72 h culture of T cells in SMG (SMG-T) or control static (Static-T) conditions, IL-2 production by the T cells was reduced in SMG-T cells compared to Static-T cells upon stimulation by phorbol 12-myristate 13-acetate (PMA) and ionomycin. Tetradecanoylphorbol Acetate 228-231 interleukin 2 Homo sapiens 90-94 27302770-3 2016 BC12 treatment confers a >95% inhibition of IL-2 secretion in phytohaemagglutinin (PHA) plus phorbol-12-myristate-13-acetate (PMA) stimulated Jurkat T cells. Tetradecanoylphorbol Acetate 96-127 interleukin 2 Homo sapiens 47-51 27647370-5 2016 This compound exhibits potent inhibitory activity toward IL-2 release in both 12-o-tetradecanoylphorbol-13-acetate (PMA)/A23187 (ionomycin) (IC50=80+-10nM) and anti-CD3/CD28-stimulated Jurkat T cells (83% inhibition at 10muM) without cytotoxic effects. Tetradecanoylphorbol Acetate 78-114 interleukin 2 Homo sapiens 57-61 27647370-5 2016 This compound exhibits potent inhibitory activity toward IL-2 release in both 12-o-tetradecanoylphorbol-13-acetate (PMA)/A23187 (ionomycin) (IC50=80+-10nM) and anti-CD3/CD28-stimulated Jurkat T cells (83% inhibition at 10muM) without cytotoxic effects. Tetradecanoylphorbol Acetate 116-119 interleukin 2 Homo sapiens 57-61 27448806-10 2016 Moreover, increased production of IL2, IFN-gamma and TNF-alpha was found in T cells of NF1 patients upon phorbol-12-myristate acetate (PMA) stimulation compared to healthy controls. Tetradecanoylphorbol Acetate 135-138 interleukin 2 Homo sapiens 34-37 24706270-8 2014 By stimulation of PBMCs with PMA/ionomycin for 6 h, more than 1-2 % of total CD8 T cells are identified as positive in terms of multifunctionality, thus producing multiple cytokines--IL-2, TNFalpha, and IFNgamma--at single-cell level in case of all healthy donors. Tetradecanoylphorbol Acetate 29-32 interleukin 2 Homo sapiens 183-187 27082871-9 2016 After stimulation with phorbol-12-myristate 13-acetate and ionomycin, the proportion of T cells producing interferon-gamma or interleukin-2 was higher in the low-IC-TAC group than in the high-IC-TAC group. Tetradecanoylphorbol Acetate 23-54 interleukin 2 Homo sapiens 126-139 26739385-4 2016 Compound 8 exhibited inhibition of the secretion of IL-2 in phytohemagglutinin (PHA) and phorbol myristate acetate (PMA) stimulated Jurkat cells, and the IC50 value was 17.2 muM. Tetradecanoylphorbol Acetate 89-114 interleukin 2 Homo sapiens 52-56 26739385-4 2016 Compound 8 exhibited inhibition of the secretion of IL-2 in phytohemagglutinin (PHA) and phorbol myristate acetate (PMA) stimulated Jurkat cells, and the IC50 value was 17.2 muM. Tetradecanoylphorbol Acetate 116-119 interleukin 2 Homo sapiens 52-56 26874672-6 2016 Suplatast also suppressed ionomycin/phorbol-12-myristate-13-acetate-induced upregulation of IL-2 gene expression in Jurkat cells, in which calcineurin (CN)/nuclear factor of activated T-cells (NFAT) signaling is known to be involved. Tetradecanoylphorbol Acetate 36-67 interleukin 2 Homo sapiens 92-96 26851025-5 2016 To induce interleukin-2 (IL2) expression, cells were stimulated with phorbol 12-myristate 13-acetate/phytohemagglutinin. Tetradecanoylphorbol Acetate 69-100 interleukin 2 Homo sapiens 10-23 26851025-5 2016 To induce interleukin-2 (IL2) expression, cells were stimulated with phorbol 12-myristate 13-acetate/phytohemagglutinin. Tetradecanoylphorbol Acetate 69-100 interleukin 2 Homo sapiens 25-28 22700433-5 2012 15d-PGJ2-G treatment decreased phorbol 12-myristate 13-acetate (PMA)/calcium ionophore (I0)-induced NFAT DNA binding to the human IL-2 promoter and nuclear NFAT2 accumulation. Tetradecanoylphorbol Acetate 31-62 interleukin 2 Homo sapiens 130-134 24049660-11 2013 The significantly increased postchallenge concentrations of IL-2, IL-8, IL-12p70, IL-13, IL-18, IFN- gamma , TNF- alpha , and TGF- beta were released by peripheral blood cells after stimulation with PMA, as compared with both their prechallenge concentrations and with the PBS control values. Tetradecanoylphorbol Acetate 199-202 interleukin 2 Homo sapiens 60-64 22700433-5 2012 15d-PGJ2-G treatment decreased phorbol 12-myristate 13-acetate (PMA)/calcium ionophore (I0)-induced NFAT DNA binding to the human IL-2 promoter and nuclear NFAT2 accumulation. Tetradecanoylphorbol Acetate 64-67 interleukin 2 Homo sapiens 130-134 22355730-3 2012 PMA/ionomycin stimulated IFN-gamma+IL-2+TNF-alpha+ CD4 T cell responses were decreased in patients with smear-positive tuberculosis compared to those with smear-negative tuberculosis. Tetradecanoylphorbol Acetate 0-3 interleukin 2 Homo sapiens 35-39 22561169-4 2012 Mn2+ at 0.1-1 mM did not significantly induce IL-2 production, whereas phorbol 12-myristate 13-acetate (PMA) at 1 muM slightly induced IL-2 production. Tetradecanoylphorbol Acetate 71-102 interleukin 2 Homo sapiens 135-139 22561169-4 2012 Mn2+ at 0.1-1 mM did not significantly induce IL-2 production, whereas phorbol 12-myristate 13-acetate (PMA) at 1 muM slightly induced IL-2 production. Tetradecanoylphorbol Acetate 104-107 interleukin 2 Homo sapiens 135-139 22561169-8 2012 These results suggest that Mn2+ promotes PMA-induced IL-2 production by inducing the production and activation of AP-1, at least in part. Tetradecanoylphorbol Acetate 41-44 interleukin 2 Homo sapiens 53-57 22160839-4 2012 Th1 and Th2 cells, levels of INF-gamma, IL-2, IL-4 and the activities of T-bet and GATA3 were significantly increased after incubation with PMA and ionomycin. Tetradecanoylphorbol Acetate 140-143 interleukin 2 Homo sapiens 40-44 21994461-8 2011 Following global stimulation with phorbol myristate acetate (PMA)-ionomycin, we noted a significant interleukin 2 (IL-2) deficit in both cervical and blood CD4 T cells from HIV-infected women compared to uninfected women, while gamma interferon (IFN-gamma) production was similar, irrespective of HIV status. Tetradecanoylphorbol Acetate 34-59 interleukin 2 Homo sapiens 100-113 21994461-8 2011 Following global stimulation with phorbol myristate acetate (PMA)-ionomycin, we noted a significant interleukin 2 (IL-2) deficit in both cervical and blood CD4 T cells from HIV-infected women compared to uninfected women, while gamma interferon (IFN-gamma) production was similar, irrespective of HIV status. Tetradecanoylphorbol Acetate 61-64 interleukin 2 Homo sapiens 100-113 21994461-8 2011 Following global stimulation with phorbol myristate acetate (PMA)-ionomycin, we noted a significant interleukin 2 (IL-2) deficit in both cervical and blood CD4 T cells from HIV-infected women compared to uninfected women, while gamma interferon (IFN-gamma) production was similar, irrespective of HIV status. Tetradecanoylphorbol Acetate 61-64 interleukin 2 Homo sapiens 115-119 21994461-8 2011 Following global stimulation with phorbol myristate acetate (PMA)-ionomycin, we noted a significant interleukin 2 (IL-2) deficit in both cervical and blood CD4 T cells from HIV-infected women compared to uninfected women, while gamma interferon (IFN-gamma) production was similar, irrespective of HIV status. Tetradecanoylphorbol Acetate 34-59 interleukin 2 Homo sapiens 115-119 20860439-7 2010 Further, we have investigated the effect of glycyrol on phorbol 12-myristate 13-acetate (PMA)/ionomycin (Io)-stimulated IL-2 expression in Jurkat cells. Tetradecanoylphorbol Acetate 56-87 interleukin 2 Homo sapiens 120-124 21460222-7 2011 It resulted in up-regulation of the transcription factor c-Jun and the activation marker CD69 as well as enhanced production of IL-2 after phorbol 12-myristate 13-acetate (PMA) and ionomycin treatment. Tetradecanoylphorbol Acetate 139-170 interleukin 2 Homo sapiens 128-132 21460222-7 2011 It resulted in up-regulation of the transcription factor c-Jun and the activation marker CD69 as well as enhanced production of IL-2 after phorbol 12-myristate 13-acetate (PMA) and ionomycin treatment. Tetradecanoylphorbol Acetate 172-175 interleukin 2 Homo sapiens 128-132 20870937-12 2010 Our results support a model in which PMA stimulation activates PKCbetaI to phosphorylate NF90-Ser(647), and this phosphorylation triggers NF90 relocation to the cytoplasm and stabilize IL-2 mRNA. Tetradecanoylphorbol Acetate 37-40 interleukin 2 Homo sapiens 185-189 20860439-7 2010 Further, we have investigated the effect of glycyrol on phorbol 12-myristate 13-acetate (PMA)/ionomycin (Io)-stimulated IL-2 expression in Jurkat cells. Tetradecanoylphorbol Acetate 89-92 interleukin 2 Homo sapiens 120-124 20508956-8 2010 Most of the syncytia were viable and expressed CD25 and IL-2 in response to activation by phorbol myristate acetate (PMA) and ionomicyn. Tetradecanoylphorbol Acetate 90-115 interleukin 2 Homo sapiens 56-60 20508956-8 2010 Most of the syncytia were viable and expressed CD25 and IL-2 in response to activation by phorbol myristate acetate (PMA) and ionomicyn. Tetradecanoylphorbol Acetate 117-120 interleukin 2 Homo sapiens 56-60 19265538-8 2009 To assess functional consequences, IL-2 production, induced by PMA and ionomycin, was determined using enzyme-linked immunosorbent spot assay (ELISpot). Tetradecanoylphorbol Acetate 63-66 interleukin 2 Homo sapiens 35-39 20846475-3 2010 IL-2 production, in contrast, was strongly inhibited in both transfectant populations stimulated by PMA plus the calcium ionophore ionomycin. Tetradecanoylphorbol Acetate 100-103 interleukin 2 Homo sapiens 0-4 17434721-6 2007 Stimulation of interleukin-2 transcriptional activity with phorbol-12-myristate-13-acetate and phytohemagglutinin caused a rapid depletion of K12Bio H4 in the gene promoter. Tetradecanoylphorbol Acetate 59-90 interleukin 2 Homo sapiens 15-28 18710607-6 2009 The tetradecanoylphorbol acetate plus ionomycin-stimulated IL-2 mRNA level started to increase after ingestion of 400 mg DHA/d, with a maximum after 800 mg intake, and was positively correlated (P < 0.003) with DHA enrichment in cell phospholipids. Tetradecanoylphorbol Acetate 4-32 interleukin 2 Homo sapiens 59-63 19038244-6 2009 In the present study, we determined that andrographolide reduced IL-2 production in Jurkat cells stimulated with phorbol myristate acetate and ionomycin (PMA/Ionomycin). Tetradecanoylphorbol Acetate 113-138 interleukin 2 Homo sapiens 65-69 19038244-6 2009 In the present study, we determined that andrographolide reduced IL-2 production in Jurkat cells stimulated with phorbol myristate acetate and ionomycin (PMA/Ionomycin). Tetradecanoylphorbol Acetate 154-157 interleukin 2 Homo sapiens 65-69 20182632-3 2009 The production of IL-2 in siRNA treated cells stimulated with PMA/ionomycin was not affected indicating an involvement of TOM1L in a pathway proximal of TCR and CD28. Tetradecanoylphorbol Acetate 62-65 interleukin 2 Homo sapiens 18-22 18507834-8 2008 The production of IL-2 in peripheral blood mononuclear cell cultures was induced by activation for 48 hr with Staphylococcal protein A (SPA) and, in parallel preparations, with the combination of phorbol ester (TPA) and calcium ionophore. Tetradecanoylphorbol Acetate 211-214 interleukin 2 Homo sapiens 18-22 18507834-12 2008 Conversely, IL-2 synthesis induced by TPA and calcium ionophore was unaltered by either type of SMT and was comparable to that in VC group at all time points. Tetradecanoylphorbol Acetate 38-41 interleukin 2 Homo sapiens 12-16 18296860-2 2008 ATRA enhanced the production of IL-2 stimulated by TPA, but suppressed that stimulated by PHA. Tetradecanoylphorbol Acetate 51-54 interleukin 2 Homo sapiens 32-36 18296860-6 2008 Since PKC- beta1 has been suggested to be important for the secretion and gene expression of IL-2 and since the activator protein-l binding site is present in the promoter of the IL-2 gene, these findings may explain the differences in ATRA"s effects on TPA- and PHA-stimulated IL-2 expression. Tetradecanoylphorbol Acetate 254-257 interleukin 2 Homo sapiens 179-183 18296860-6 2008 Since PKC- beta1 has been suggested to be important for the secretion and gene expression of IL-2 and since the activator protein-l binding site is present in the promoter of the IL-2 gene, these findings may explain the differences in ATRA"s effects on TPA- and PHA-stimulated IL-2 expression. Tetradecanoylphorbol Acetate 254-257 interleukin 2 Homo sapiens 179-183 18992303-4 2009 Promoter activities of IL-2, nuclear factor of activated T cells (NFAT), and activator protein-1 (AP-1), after 6 h stimulation with PMA and ionomycin, gradually decreased in high glucose cultures to approximately 20% of those in normal glucose at 12 weeks. Tetradecanoylphorbol Acetate 132-135 interleukin 2 Homo sapiens 23-27 17552910-5 2007 Gliovirin also significantly reduced TPA/ionomycin-induced IL-2 mRNA levels and synthesis in Jurkat cells at low micromolar concentrations. Tetradecanoylphorbol Acetate 37-40 interleukin 2 Homo sapiens 59-63 17344653-9 2007 In accordance with adult data, the percentage of neonatal lymphocytes producing IL-2 after phorbol 12-myristate 13-acetate/ionomycin stimulation was dose-dependently reduced (e.g., 41.3% inhibition, p = 0.001, 20 mM vitamin C), while the percentage of TNF-alpha producing lymphocytes was mildly stimulated (e.g., 20.8% increase, p = 0.003, 20 mM vitamin C). Tetradecanoylphorbol Acetate 91-122 interleukin 2 Homo sapiens 80-84 17152808-7 2006 Negative correlation was found between expression of Interleukin 2, 4, 13 after PMA/ionomycin and allergen stimulation (p > 0.05) into two groups. Tetradecanoylphorbol Acetate 80-83 interleukin 2 Homo sapiens 53-66 16278302-4 2006 beta-agonist and PGE2 also inhibited phorbol myristate acetate (PMA) + calcimycin-stimulated IFN-gamma and IL-2 (but not IL-13) production, suggesting that upstream CD3-initiated signaling is not the sole locus of PKA actions. Tetradecanoylphorbol Acetate 37-62 interleukin 2 Homo sapiens 107-111 16278302-4 2006 beta-agonist and PGE2 also inhibited phorbol myristate acetate (PMA) + calcimycin-stimulated IFN-gamma and IL-2 (but not IL-13) production, suggesting that upstream CD3-initiated signaling is not the sole locus of PKA actions. Tetradecanoylphorbol Acetate 64-67 interleukin 2 Homo sapiens 107-111 17097050-3 2006 Inhibition of AMPK by compound C, a specific inhibitor of AMPK or small interfering RNA of AMPKalpha1 suppressed IL-2 production in Jurkat T cells and peripheral blood lymphocytes stimulated with PMA plus ionomycin (PMA/Io) or with monoclonal anti-CD3 plus anti-CD28. Tetradecanoylphorbol Acetate 196-199 interleukin 2 Homo sapiens 113-117 17097050-4 2006 We then showed that AMPK inhibition reduced PMA/Io-induced IL-2 mRNA expression and IL-2 promoter activation. Tetradecanoylphorbol Acetate 44-47 interleukin 2 Homo sapiens 59-63 17097050-4 2006 We then showed that AMPK inhibition reduced PMA/Io-induced IL-2 mRNA expression and IL-2 promoter activation. Tetradecanoylphorbol Acetate 44-47 interleukin 2 Homo sapiens 84-88 16055081-10 2005 Zn2+ also suppressed IL-2 mRNA expression induced by phorbol ester (PMA) and ionomycin. Tetradecanoylphorbol Acetate 68-71 interleukin 2 Homo sapiens 21-25 16309325-3 2005 Of these compounds, geniposide (3), 6alpha-hydroxygeniposide (5), ixoroside (7), and shanzhiside (8) showed significant inhibition of IL-2 secretion by phorbol myristate acetate and anti-CD28 monoclonal antibody co-stimulated activation of human peripheral blood T cells. Tetradecanoylphorbol Acetate 152-177 interleukin 2 Homo sapiens 134-138 16358840-6 2005 Blood sample was obtained from patients and examined for the expression of Interleukin 2 and 4 by intracellular staining procedure, after stimulation with PMA (phorbol 12-myristate 13-acetate) and allergen (D. pteronyssimus, Allergopharma). Tetradecanoylphorbol Acetate 155-158 interleukin 2 Homo sapiens 75-94 16358840-6 2005 Blood sample was obtained from patients and examined for the expression of Interleukin 2 and 4 by intracellular staining procedure, after stimulation with PMA (phorbol 12-myristate 13-acetate) and allergen (D. pteronyssimus, Allergopharma). Tetradecanoylphorbol Acetate 160-191 interleukin 2 Homo sapiens 75-94 15090250-3 2003 Treatment of anergic Jurkat cells with the combination of the phorbol ester, PMA, and ionomycin restored IL-2 production in cells rendered anergic by both mechanisms. Tetradecanoylphorbol Acetate 77-80 interleukin 2 Homo sapiens 105-109 15588914-3 2005 DON (62.5-500 ng/ml) also significantly upregulated IL-2 and IL-8 production following prestimulation with phorbol myristate acetate and ionomycin. Tetradecanoylphorbol Acetate 107-132 interleukin 2 Homo sapiens 52-56 15671558-6 2005 RESULTS: Frequencies of CD3+ T cells producing IFN-gamma (type 1 T cells) in response to phorbol myristate acetate/ionomycin increased (median, 1.8-fold) in patients receiving IL-2 plus HDC but not in those receiving IL-2 alone (P < 0.01 for comparison between arms). Tetradecanoylphorbol Acetate 89-114 interleukin 2 Homo sapiens 176-180 15671558-6 2005 RESULTS: Frequencies of CD3+ T cells producing IFN-gamma (type 1 T cells) in response to phorbol myristate acetate/ionomycin increased (median, 1.8-fold) in patients receiving IL-2 plus HDC but not in those receiving IL-2 alone (P < 0.01 for comparison between arms). Tetradecanoylphorbol Acetate 89-114 interleukin 2 Homo sapiens 217-221 15459838-6 2004 In athletes, 7.9 % of cells responding to non-specific (PMA and ionomycin) stimulation produced IL-2 versus 3.9 % of responding cells in non-athletes (p < 0.05). Tetradecanoylphorbol Acetate 56-59 interleukin 2 Homo sapiens 96-100 15271375-5 2004 Simultaneously, the number of lymphocytes producing IL-2 after PMA/ionomycin stimulation was dose dependently reduced (e.g., 24.2% inhibition, p < 0.005, 20 mM vitamin C). Tetradecanoylphorbol Acetate 63-66 interleukin 2 Homo sapiens 52-56 15034076-9 2004 Stimulation of T cells from nonpregnant females with PMA/ionomycin resulted in IkappaBalpha degradation, p65 translocation, and subsequent production of the Th1 cytokines IFN-gamma and IL-2. Tetradecanoylphorbol Acetate 53-56 interleukin 2 Homo sapiens 185-189 15910743-2 2005 AICAR inhibited IL-2 production in Jurkat T cells and peripheral blood lymphocytes activated with PMA plus ionomycin (PMA/Io) or with monoclonal anti-CD3 plus anti-CD28. Tetradecanoylphorbol Acetate 98-101 interleukin 2 Homo sapiens 16-20 15910743-2 2005 AICAR inhibited IL-2 production in Jurkat T cells and peripheral blood lymphocytes activated with PMA plus ionomycin (PMA/Io) or with monoclonal anti-CD3 plus anti-CD28. Tetradecanoylphorbol Acetate 118-121 interleukin 2 Homo sapiens 16-20 15910743-4 2005 We then showed that AICAR inhibited PMA/Io-induced IL-2 mRNA expression and IL-2 promoter activation. Tetradecanoylphorbol Acetate 36-39 interleukin 2 Homo sapiens 51-55 15910743-4 2005 We then showed that AICAR inhibited PMA/Io-induced IL-2 mRNA expression and IL-2 promoter activation. Tetradecanoylphorbol Acetate 36-39 interleukin 2 Homo sapiens 76-80 15968830-3 2005 Judging by the cytokine production of interleukin-2 and interferon-gamma in peripheral blood mononuclear cells stimulated by phorbol-myristate-acetate (PMA) and ionomycin, immunosuppressive drugs given for rheumatic disorders during pregnancy do not induce significant immunosuppression in babies. Tetradecanoylphorbol Acetate 125-150 interleukin 2 Homo sapiens 38-51 15968830-3 2005 Judging by the cytokine production of interleukin-2 and interferon-gamma in peripheral blood mononuclear cells stimulated by phorbol-myristate-acetate (PMA) and ionomycin, immunosuppressive drugs given for rheumatic disorders during pregnancy do not induce significant immunosuppression in babies. Tetradecanoylphorbol Acetate 152-155 interleukin 2 Homo sapiens 38-51 15974445-2 2005 In the present study, lipopolysaccharide (LPS) and phorbol 12-myristate 13-acetate/phytohemagglutinin (PMA/PHA) were used as stimulants for RAW 264.7 macrophages and human peripheral blood mononuclear cell (hPBMC), and tumor necrosis factor (TNF)-alpha and interleukin (IL)-2 productions were measured. Tetradecanoylphorbol Acetate 51-82 interleukin 2 Homo sapiens 257-275 15477007-6 2004 We found that both inhibited the increase in expression of many of the genes, including IL-2 and MKP-2, that were induced early after stimulation of lymphocytes with phorbol myristate acetate and ionomycin. Tetradecanoylphorbol Acetate 166-191 interleukin 2 Homo sapiens 88-92 15271977-8 2004 We examined the function of this Stat5-binding motif by transfecting human peripheral blood mononuclear cells with -3.6 kb of IFNG-luciferase constructs and found that phorbol 12-myristate 13-acetate/ionomycin-induced transcription was augmented by IL-2 treatment. Tetradecanoylphorbol Acetate 168-199 interleukin 2 Homo sapiens 249-253 15006553-5 2004 In the T cells stimulated with mitogenic lectin phytohemagglutinin (PHA) in conjunction with phorbol myristate acetate (PMA), 6-formylpterin suppressed the NF-kappaB-dependent transcription, the production of cytokines (IFN-gamma and IL-2) and the cell proliferation. Tetradecanoylphorbol Acetate 93-118 interleukin 2 Homo sapiens 234-238 15006553-5 2004 In the T cells stimulated with mitogenic lectin phytohemagglutinin (PHA) in conjunction with phorbol myristate acetate (PMA), 6-formylpterin suppressed the NF-kappaB-dependent transcription, the production of cytokines (IFN-gamma and IL-2) and the cell proliferation. Tetradecanoylphorbol Acetate 120-123 interleukin 2 Homo sapiens 234-238 15008989-7 2004 In contrast, after 4 h of activation with PMA and ionomycin, the percentage of T cells producing high levels of IL-2 (M2) was greater in CF patients (P = 0.02). Tetradecanoylphorbol Acetate 42-45 interleukin 2 Homo sapiens 112-116 12595531-9 2003 Furthermore, T cells treated with soluble anti-TCR antibody produced IL-2 when phorbol 12-myristate 13-acetate, which activates ERK, was present in the culture medium 2-6 h after the start of stimulation. Tetradecanoylphorbol Acetate 79-110 interleukin 2 Homo sapiens 69-73 11585388-7 2001 Exposure of preactivated T cells to IL-2 also inhibited subsequent responses to the mitogenic combination of PMA, ionomycin, and IL-2 without enhancing cell death. Tetradecanoylphorbol Acetate 109-112 interleukin 2 Homo sapiens 36-40 12657248-6 2002 Furthermore, IL-2, IL-4, IL-5, and IFN-gamma secretion by Hut 78 cells or CD3(+) T cells stimulated with PMA plus ionomycin or anti-CD3 antibody plus PMA were inhibited in a concentration-dependent manner by BTPs. Tetradecanoylphorbol Acetate 150-153 interleukin 2 Homo sapiens 13-17 12421966-5 2002 Both proteins inhibited CD3(+)/CD4(+) lymphocyte proliferation induced by PMA and ionomycin in an IL-2-independent manner. Tetradecanoylphorbol Acetate 74-77 interleukin 2 Homo sapiens 98-102 12467977-3 2002 In the presence of AGC10 the cytoplasmic levels of IL-2 protein of CD4(+) and CD8(+) blood lymphocytes stimulated with phorbol myristate acetate (PMA) plus ionomycin were markedly reduced. Tetradecanoylphorbol Acetate 119-144 interleukin 2 Homo sapiens 51-55 12467977-3 2002 In the presence of AGC10 the cytoplasmic levels of IL-2 protein of CD4(+) and CD8(+) blood lymphocytes stimulated with phorbol myristate acetate (PMA) plus ionomycin were markedly reduced. Tetradecanoylphorbol Acetate 146-149 interleukin 2 Homo sapiens 51-55 12133875-7 2002 Intracellular expression of IFN-gamma, IL-2, and IL-4 was detected in CD4(+) and CD8(+) T cells after stimulation with phorbol 12-myristate 13-acetate and ionomycin. Tetradecanoylphorbol Acetate 119-150 interleukin 2 Homo sapiens 39-43 12036883-6 2002 Treatment with rapamycin blocked IL-2 production after activation of human peripheral blood T cells with phorbol ester (PMA) and anti-CD28 (CsA-resistant pathway), whereas this drug did not have any effect on PMA plus ionomycin stimulation (CsA-sensitive pathway). Tetradecanoylphorbol Acetate 120-123 interleukin 2 Homo sapiens 33-37 10685665-10 2000 Gangliosides isolated from tumor supernatants blocked the production of IL-2 and IFN-gamma in response to ionomycin plus phorbol myristate acetate stimulation. Tetradecanoylphorbol Acetate 121-146 interleukin 2 Homo sapiens 72-76 11074886-14 2000 Release of IL-2 and IL-4 from peripheral blood mononuclear cell fractions stimulated with phorbolmyristateacetate and ionomycin was significantly increased in patients with trauma but not from those stimulated with toxic shock syndrome toxin-1. Tetradecanoylphorbol Acetate 90-113 interleukin 2 Homo sapiens 11-15 11041251-3 2000 Pretreatment of the cells with muscarinic receptor agonist, oxotremorine M (Oxo-M), enhanced IL-2 production induced by phorbol 12-myristate 13-acetate (PMA)/A23187, while Oxo-M by itself did not affect IL-2 production. Tetradecanoylphorbol Acetate 120-151 interleukin 2 Homo sapiens 93-97 11041251-3 2000 Pretreatment of the cells with muscarinic receptor agonist, oxotremorine M (Oxo-M), enhanced IL-2 production induced by phorbol 12-myristate 13-acetate (PMA)/A23187, while Oxo-M by itself did not affect IL-2 production. Tetradecanoylphorbol Acetate 153-156 interleukin 2 Homo sapiens 93-97 11007162-4 2000 RESULTS: Activation of lymphocytes with PMA + Ionomycin induced the expression of IL-2 and IFN-gamma in each lymphocyte population. Tetradecanoylphorbol Acetate 40-43 interleukin 2 Homo sapiens 82-86 11678905-4 2001 Following re-stimulation in vitro with PMA and ionomycin, CD4+ T cells produced IFNgamma, TNFalpha, TNFbeta, IL-2, IL-4, IL-10 and IL-13. Tetradecanoylphorbol Acetate 39-42 interleukin 2 Homo sapiens 109-113 11795505-6 2001 We also investigated the effect of PYC on IL-2 gene expression in phorbol 12-myristate 13acetate plus ionomycin (PMA/Io)-stimulated human T-cell line Jurkat E6.1. Tetradecanoylphorbol Acetate 66-96 interleukin 2 Homo sapiens 42-46 11795505-6 2001 We also investigated the effect of PYC on IL-2 gene expression in phorbol 12-myristate 13acetate plus ionomycin (PMA/Io)-stimulated human T-cell line Jurkat E6.1. Tetradecanoylphorbol Acetate 113-116 interleukin 2 Homo sapiens 42-46 11795505-7 2001 PYC inhibited the PMA/Io-induced IL-2 mRNA expression. Tetradecanoylphorbol Acetate 18-21 interleukin 2 Homo sapiens 33-37 11795505-10 2001 We also found that PYC can destabilize PMA/Io-induced IL-2 mRNA by posttranscriptional regulation. Tetradecanoylphorbol Acetate 39-42 interleukin 2 Homo sapiens 54-58 11034109-3 2000 Melatonin enhances IL-2 production by Jurkat cells activated by either phytohemagglutinin (PHA) or phorbol myristate acetate (PMA). Tetradecanoylphorbol Acetate 99-124 interleukin 2 Homo sapiens 19-23 11034109-3 2000 Melatonin enhances IL-2 production by Jurkat cells activated by either phytohemagglutinin (PHA) or phorbol myristate acetate (PMA). Tetradecanoylphorbol Acetate 126-129 interleukin 2 Homo sapiens 19-23 10985305-4 2000 Stimulation of these cells with anti-CD28 antibody, and either phorbol 12-myristate 13-acetate (PMA) or anti-CD3, activates signal transduction pathways and results in IL-2 production and IL-2 receptor alpha-chain (CD25) expression. Tetradecanoylphorbol Acetate 63-94 interleukin 2 Homo sapiens 168-172 10985305-4 2000 Stimulation of these cells with anti-CD28 antibody, and either phorbol 12-myristate 13-acetate (PMA) or anti-CD3, activates signal transduction pathways and results in IL-2 production and IL-2 receptor alpha-chain (CD25) expression. Tetradecanoylphorbol Acetate 63-94 interleukin 2 Homo sapiens 188-192 10985305-4 2000 Stimulation of these cells with anti-CD28 antibody, and either phorbol 12-myristate 13-acetate (PMA) or anti-CD3, activates signal transduction pathways and results in IL-2 production and IL-2 receptor alpha-chain (CD25) expression. Tetradecanoylphorbol Acetate 96-99 interleukin 2 Homo sapiens 168-172 10985305-4 2000 Stimulation of these cells with anti-CD28 antibody, and either phorbol 12-myristate 13-acetate (PMA) or anti-CD3, activates signal transduction pathways and results in IL-2 production and IL-2 receptor alpha-chain (CD25) expression. Tetradecanoylphorbol Acetate 96-99 interleukin 2 Homo sapiens 188-192 11268366-3 2000 Melatonin enhances IL-2 production by Jurkat cells activated by either phytohemagglutinin (PHA) or phorbol myristate acetate (PMA). Tetradecanoylphorbol Acetate 99-124 interleukin 2 Homo sapiens 19-23 10644863-9 2000 A significant number of IFN-gamma- and IL-2-expressing T lymphocytes were only detected after 18 hours of PMA/ionomycin stimulation. Tetradecanoylphorbol Acetate 106-109 interleukin 2 Homo sapiens 39-43 11268366-3 2000 Melatonin enhances IL-2 production by Jurkat cells activated by either phytohemagglutinin (PHA) or phorbol myristate acetate (PMA). Tetradecanoylphorbol Acetate 126-129 interleukin 2 Homo sapiens 19-23 10655565-6 2000 Other known stimuli for NK cells (IL-2 and CD16 monoclonal antibody and incubation with K562, the NK-sensitive cell line) promoted IFN-gamma and TNF-alpha production in NK cells to a lesser extent than did PMA and ionomycin stimulation. Tetradecanoylphorbol Acetate 206-209 interleukin 2 Homo sapiens 34-38 10419652-3 1999 The optimal time for positive identification of IL-2 in both blood and BAL was 5 h after PMA/ionomycin stimulation, whereas the first peak for IFN-gamma was found after 5 h in blood but after only 3 h in BAL. Tetradecanoylphorbol Acetate 89-92 interleukin 2 Homo sapiens 48-52 10468798-5 1999 SDZ ASM 981 inhibits the phorbol myristate acetate/phytohaemagglutinin-stimulated transcription of a reporter gene coupled to the human IL-2 promoter in the human T-cell line Jurkat and the IgE/antigen-mediated transcription of a reporter gene coupled to the human tumour necrosis factor (TNF)-alpha promoter in the murine mast-cell line CPII. Tetradecanoylphorbol Acetate 25-50 interleukin 2 Homo sapiens 136-140 10393966-4 1999 As defined by IL-2 generation, activation of T cells stimulated with classical mitogens [phorbol 12-myristate 13-acetate (PMA) + anti-CD3, PMA + phytohemagglutinin, and PMA + ionomycin] is unaffected by the expression of Nef. Tetradecanoylphorbol Acetate 89-120 interleukin 2 Homo sapiens 14-18 10580999-3 1999 We show that blocking of IL-2 synthesis by Cyclosporin A (CsA) suppressed both the Concanavalin A (Con A)- and phorbol myristate acetate (PMA)/ionomycin-induced proliferation of T cells. Tetradecanoylphorbol Acetate 111-136 interleukin 2 Homo sapiens 25-29 10580999-3 1999 We show that blocking of IL-2 synthesis by Cyclosporin A (CsA) suppressed both the Concanavalin A (Con A)- and phorbol myristate acetate (PMA)/ionomycin-induced proliferation of T cells. Tetradecanoylphorbol Acetate 138-141 interleukin 2 Homo sapiens 25-29 10525317-6 1999 Compared to controls, rheumatoid arthritis patients, treated with salazopyrin, showed an increased number of IL-2-producing T-helper and T-suppressor/cytotoxic lymphocytes after in vitro stimulation with PMA and ionomycin (P=0.01). Tetradecanoylphorbol Acetate 204-207 interleukin 2 Homo sapiens 109-113 9882624-5 1999 The inhibition of IL-2 production was observed in the CD3(+) T-lymphocyte cytoplasm as early as 4 h after activation by PMA+ionomycin. Tetradecanoylphorbol Acetate 120-123 interleukin 2 Homo sapiens 18-22 9973532-8 1999 Cross-linking the intact monomorphic HLA-A,B,C epitope or the polymorphic HLA-B7 epitope induced IL-2 production upon costimulation with PMA. Tetradecanoylphorbol Acetate 137-140 interleukin 2 Homo sapiens 97-101 10191628-4 1999 Flow cytometric detection of intracellular cytokines in tonsillar mononuclear cells stimulated with PMA and ionomycin revealed that CD3 cells produced IL-1 alpha, IL-2, IL-4, IL-8, IFN-gamma and TNF-alpha, and CD19 cells produced IL-1 alpha, IL-6, IL-8 and TFN-alpha. Tetradecanoylphorbol Acetate 100-103 interleukin 2 Homo sapiens 163-167 10224109-2 1999 PG490 inhibits interleukin(IL)-2 expression by normal human peripheral blood lymphocytes stimulated with phorbol 12-myristate 13-acetate (PMA) and antibody to CD3 (IC50 of 10 ng/ml), and with PMA and ionomycin (Iono, IC50 of 40 ng/ml). Tetradecanoylphorbol Acetate 105-136 interleukin 2 Homo sapiens 15-32 10224109-2 1999 PG490 inhibits interleukin(IL)-2 expression by normal human peripheral blood lymphocytes stimulated with phorbol 12-myristate 13-acetate (PMA) and antibody to CD3 (IC50 of 10 ng/ml), and with PMA and ionomycin (Iono, IC50 of 40 ng/ml). Tetradecanoylphorbol Acetate 138-141 interleukin 2 Homo sapiens 15-32 10229086-5 1999 Consistent with the presence of AP-1 proteins within the IL-2 NFAT complex, PDTC strongly enhanced phorbol 12-myristate 13-acetate/phytohemagglutinin-induced NFAT binding to the IL-2 NFAT enhancer and transcriptional activity of a reporter plasmid driven by this NFAT enhancer. Tetradecanoylphorbol Acetate 99-130 interleukin 2 Homo sapiens 57-61 10229086-5 1999 Consistent with the presence of AP-1 proteins within the IL-2 NFAT complex, PDTC strongly enhanced phorbol 12-myristate 13-acetate/phytohemagglutinin-induced NFAT binding to the IL-2 NFAT enhancer and transcriptional activity of a reporter plasmid driven by this NFAT enhancer. Tetradecanoylphorbol Acetate 99-130 interleukin 2 Homo sapiens 178-182 9862370-3 1998 Addition of various co-stimuli (phorbol 12-myristate 13-acetate or monoclonal antibodies to CD3 or CD2) increases the CD82-induced morphological alterations and, reciprocally, CD82 engagement synergizes with these stimuli to induce T cell activation as indicated by both primary tyrosine phosphorylation and IL-2 production. Tetradecanoylphorbol Acetate 32-63 interleukin 2 Homo sapiens 308-312 9874668-5 1999 Upon activation with phorbol 12-myristate 13-acetate (PMA), the numbers of T cells synthesizing IL-2 were similar for all study groups. Tetradecanoylphorbol Acetate 21-52 interleukin 2 Homo sapiens 96-100 9874668-5 1999 Upon activation with phorbol 12-myristate 13-acetate (PMA), the numbers of T cells synthesizing IL-2 were similar for all study groups. Tetradecanoylphorbol Acetate 54-57 interleukin 2 Homo sapiens 96-100 9763600-7 1998 Strikingly, IL-2 production induced by PMA and ionomycin was unaffected by C3 treatment. Tetradecanoylphorbol Acetate 39-42 interleukin 2 Homo sapiens 12-16 9682002-6 1998 Lymphocytes which have been exposed to dexamethasone in vitro retained the ability to express CD40L after incubation in medium alone for 48 h. Dexamethasone also inhibited PMA/ionomycin induced IL-2 and IFN-gamma production but not CD25 and CD69 expression. Tetradecanoylphorbol Acetate 172-175 interleukin 2 Homo sapiens 194-198 9808174-4 1998 Upon phorbol 12-myristate 13-acetate + ionomycin stimulation, accumulation of cytoplasmic IL-2 was similar to that observed in freshly isolated cells, but no IL-4- or IFN-gamma-positive cells were detected. Tetradecanoylphorbol Acetate 5-36 interleukin 2 Homo sapiens 90-94 9670939-9 1998 The intracellular incorporation of Abs specific for c-Fos and c-Jun family members by scrape loading inhibited the production and intracellular accumulation of IL-2 within 6 h of costimulation with PMA/ionomycin, or costimulation by CD28 and CD3 ligation. Tetradecanoylphorbol Acetate 198-201 interleukin 2 Homo sapiens 160-164 9603471-4 1998 In contrast, the phorbol 12-myristate 13-acetate (PMA)-induced IL-10 production and CD69 expression, and the ionomycin plus PMA-induced IL-2 production are not affected. Tetradecanoylphorbol Acetate 124-127 interleukin 2 Homo sapiens 136-140 9553058-1 1998 T cell activation leads via multiple intracellular signaling pathways to rapid induction of interleukin-2 (IL-2) expression, which can be mimicked by costimulation with 12-O-tetradecanoylphorbol-13-acetate (TPA) and ionomycin. Tetradecanoylphorbol Acetate 169-205 interleukin 2 Homo sapiens 92-105 9583867-3 1998 METHODS: We determined the percentages of CD4+ and CD8+ lymphocytes producing IL-2 upon stimulation by phorbol myristate acetate and calcium ionophore in whole blood culture, using immunostaining of intracytoplasmatic and membrane markers, followed by multiparameter flow cytometry. Tetradecanoylphorbol Acetate 103-128 interleukin 2 Homo sapiens 78-82 9553058-2 1998 We have identified a distal IL-2 enhancer regulated by the Raf-MEK-ERK signaling pathway, which can be induced by TPA/ionomycin treatment. Tetradecanoylphorbol Acetate 114-117 interleukin 2 Homo sapiens 28-32 9553058-1 1998 T cell activation leads via multiple intracellular signaling pathways to rapid induction of interleukin-2 (IL-2) expression, which can be mimicked by costimulation with 12-O-tetradecanoylphorbol-13-acetate (TPA) and ionomycin. Tetradecanoylphorbol Acetate 169-205 interleukin 2 Homo sapiens 107-111 9553058-1 1998 T cell activation leads via multiple intracellular signaling pathways to rapid induction of interleukin-2 (IL-2) expression, which can be mimicked by costimulation with 12-O-tetradecanoylphorbol-13-acetate (TPA) and ionomycin. Tetradecanoylphorbol Acetate 207-210 interleukin 2 Homo sapiens 92-105 9553058-1 1998 T cell activation leads via multiple intracellular signaling pathways to rapid induction of interleukin-2 (IL-2) expression, which can be mimicked by costimulation with 12-O-tetradecanoylphorbol-13-acetate (TPA) and ionomycin. Tetradecanoylphorbol Acetate 207-210 interleukin 2 Homo sapiens 107-111 9409644-7 1997 In activated CD8- T cells, IL-2 and interferon-gamma (IFN-gamma) were optimally induced after 10 h stimulation with phorbol 12-myristate acetate (PMA)/ionomycin, and in CD8+ T cells IL-2 was optimally induced after 10 h and IFN-gamma after 6 h. The levels of IL-2 and IFN-gamma in CD8+ and CD8- T cells in four healthy individuals were consistent on four occasions over a 3-month period. Tetradecanoylphorbol Acetate 146-149 interleukin 2 Homo sapiens 27-31 9111316-8 1997 In contrast to ionomycin treatment, exposure of cells to phorbol myristate acetate (PMA) plus anti-CD28 did not induce NFATc, indicating that under these conditions, interleukin-2 synthesis by these cells is apparently independent of NFATc. Tetradecanoylphorbol Acetate 57-82 interleukin 2 Homo sapiens 166-179 9199340-4 1997 The RE/AP composite element is a site for signal integration within the IL-2 promoter, since its activation is dependent on at least two separate signalling pathways being activated, through the T-cell receptor, CD28, and/or phorbol myristate acetate. Tetradecanoylphorbol Acetate 225-250 interleukin 2 Homo sapiens 72-76 9271352-9 1997 In Jurkat T cells activated by phorbol-12-myristate-13-acetate and phytohemagglutinin, CCK-8 induced IL-2 expression. Tetradecanoylphorbol Acetate 31-62 interleukin 2 Homo sapiens 101-105 9189762-12 1997 Second, interleukin-2 production after TCR/CD3 engagement and TCR/CD3 down-modulation in response to phorbol myristate acetate were shown to be comparable to wild-type Jurkat cells. Tetradecanoylphorbol Acetate 101-126 interleukin 2 Homo sapiens 8-21 9207756-0 1997 Effect of phorbol myristate acetate (PMA) and ionophore A23187 on interleukin-2 levels and proliferation of activated T lymphocytes from patients with lepromatous leprosy. Tetradecanoylphorbol Acetate 10-35 interleukin 2 Homo sapiens 66-79 9207756-10 1997 It can be concluded that the use of PMA, analogous to DAG, and ionophore A23187 (calcium increaser) in cultures of mitogen-activated T lymphocytes from LL patients induced the expression of the IL-2 gene, thus correcting the inadequate proliferation of T cells from LL patients. Tetradecanoylphorbol Acetate 36-39 interleukin 2 Homo sapiens 194-198 9207756-0 1997 Effect of phorbol myristate acetate (PMA) and ionophore A23187 on interleukin-2 levels and proliferation of activated T lymphocytes from patients with lepromatous leprosy. Tetradecanoylphorbol Acetate 37-40 interleukin 2 Homo sapiens 66-79 8900312-9 1996 Following stimulation with ionomycin and PMA, high doses (10(-6) M) of Dex inhibited the activity of the IL-2 promoter (approximately 50% inhibition). Tetradecanoylphorbol Acetate 41-44 interleukin 2 Homo sapiens 105-109 9124379-3 1997 IL-2 mRNA is present constitutively in AEC and is enhanced twofold after stimulation with phorbol 12-myristate 13-acetate (PMA; 20 ng/ml) + histamine (2 mM). Tetradecanoylphorbol Acetate 90-121 interleukin 2 Homo sapiens 0-4 9124379-3 1997 IL-2 mRNA is present constitutively in AEC and is enhanced twofold after stimulation with phorbol 12-myristate 13-acetate (PMA; 20 ng/ml) + histamine (2 mM). Tetradecanoylphorbol Acetate 123-126 interleukin 2 Homo sapiens 0-4 9124379-4 1997 Normal human AEC secrete IL-2 at rest (7 pg/ml), and IL-2 secretion is increased threefold after stimulation with PMA + histamine; this increase is inhibited by dexamethasone and diphenhydramine. Tetradecanoylphorbol Acetate 114-117 interleukin 2 Homo sapiens 53-57 9124379-5 1997 Transcriptional regulation of IL-2 was investigated with a transgenic human AEC line, 16HBE/IL-2 luciferase; there is constitutive IL-2 transcription at rest, and IL-2 transcription is enhanced 8-fold by PMA and 25-fold by PMA + histamine. Tetradecanoylphorbol Acetate 204-207 interleukin 2 Homo sapiens 30-34 8660843-4 1996 Stimulation of Jurkat cells with ionomycin and PMA in the presence of PGE2 inhibited the IL-2- but not the IL-4-promoter activity. Tetradecanoylphorbol Acetate 47-50 interleukin 2 Homo sapiens 89-93 8877124-5 1996 Upon stimulation with phytohemagglutinin (PHA) plus phorbol-12-myristate-13-acetate (PMA), RP children produced less IL-2 (P < 0.01) and IFN-gamma (P < 0.02) than SR children and also expressed significantly less IFN-gamma mRNA (P < 0.01) than SR children. Tetradecanoylphorbol Acetate 52-83 interleukin 2 Homo sapiens 117-121 8648737-2 1996 We have found that the human T leukemia/lymphotropic virus type 1 viral protein Tax can also strongly costimulate expression of interleukin-2 (IL-2), IL-3, and granulocyte-macrophage colony-stimulating factor (GM-CSF) mRNA in T cells activated with the phorbol ester phorbol myristate acetate (PMA) and calcium ionophore, which can mimic activation through the antigen specific T-cell receptor. Tetradecanoylphorbol Acetate 267-292 interleukin 2 Homo sapiens 128-141 8693291-2 1996 Phorbol-myristate-acetate (PMA), an activator of protein kinase C (PKC), also induced down-regulation of IL-2 responsiveness. Tetradecanoylphorbol Acetate 0-25 interleukin 2 Homo sapiens 105-109 8693291-2 1996 Phorbol-myristate-acetate (PMA), an activator of protein kinase C (PKC), also induced down-regulation of IL-2 responsiveness. Tetradecanoylphorbol Acetate 27-30 interleukin 2 Homo sapiens 105-109 8648737-2 1996 We have found that the human T leukemia/lymphotropic virus type 1 viral protein Tax can also strongly costimulate expression of interleukin-2 (IL-2), IL-3, and granulocyte-macrophage colony-stimulating factor (GM-CSF) mRNA in T cells activated with the phorbol ester phorbol myristate acetate (PMA) and calcium ionophore, which can mimic activation through the antigen specific T-cell receptor. Tetradecanoylphorbol Acetate 294-297 interleukin 2 Homo sapiens 128-141 8648737-2 1996 We have found that the human T leukemia/lymphotropic virus type 1 viral protein Tax can also strongly costimulate expression of interleukin-2 (IL-2), IL-3, and granulocyte-macrophage colony-stimulating factor (GM-CSF) mRNA in T cells activated with the phorbol ester phorbol myristate acetate (PMA) and calcium ionophore, which can mimic activation through the antigen specific T-cell receptor. Tetradecanoylphorbol Acetate 267-292 interleukin 2 Homo sapiens 143-147 8648737-2 1996 We have found that the human T leukemia/lymphotropic virus type 1 viral protein Tax can also strongly costimulate expression of interleukin-2 (IL-2), IL-3, and granulocyte-macrophage colony-stimulating factor (GM-CSF) mRNA in T cells activated with the phorbol ester phorbol myristate acetate (PMA) and calcium ionophore, which can mimic activation through the antigen specific T-cell receptor. Tetradecanoylphorbol Acetate 294-297 interleukin 2 Homo sapiens 143-147 8625537-9 1996 Similar but transient inhibition of most T-cell products (IL-2, IL-3, IL-4, IL-5, IL-10, TNF-beta and GM-CSF) was noted in the PMA/ionomycin-containing cultures. Tetradecanoylphorbol Acetate 127-130 interleukin 2 Homo sapiens 58-62 8648737-3 1996 Reporter constructs also showed strong synergy between both stimuli and showed that Tax and the PMA-Ca2+ ionophore act through different regions of the IL-2 and GM-CSF genes. Tetradecanoylphorbol Acetate 96-99 interleukin 2 Homo sapiens 152-156 8648737-6 1996 Tax protein mutants, however, showed that a pathway(s) other than NF-kappaB/rel induction could also cooperate with the PMA-Ca2+ ionophore to activate the GM-CSF and IL-2 genes. Tetradecanoylphorbol Acetate 120-123 interleukin 2 Homo sapiens 166-170 8620546-2 1996 In the current studies, IL-2 production by T cells from elderly (mean 78 years) and young (mean 37 years) humans was measured in cultures stimulated with PHA, PHA plus PMA, crosslinked anti-CD3 mAB OKT3 plus PMA, or PMA plus ionomycin. Tetradecanoylphorbol Acetate 168-171 interleukin 2 Homo sapiens 24-28 8620546-2 1996 In the current studies, IL-2 production by T cells from elderly (mean 78 years) and young (mean 37 years) humans was measured in cultures stimulated with PHA, PHA plus PMA, crosslinked anti-CD3 mAB OKT3 plus PMA, or PMA plus ionomycin. Tetradecanoylphorbol Acetate 208-211 interleukin 2 Homo sapiens 24-28 8620546-2 1996 In the current studies, IL-2 production by T cells from elderly (mean 78 years) and young (mean 37 years) humans was measured in cultures stimulated with PHA, PHA plus PMA, crosslinked anti-CD3 mAB OKT3 plus PMA, or PMA plus ionomycin. Tetradecanoylphorbol Acetate 208-211 interleukin 2 Homo sapiens 24-28 8599837-6 1996 Under the same conditions, only 0.1 microgram/ml of CsA inhibited by >95% the transactivation of the IL-2 promoter in response to ionomycin and PMA. Tetradecanoylphorbol Acetate 147-150 interleukin 2 Homo sapiens 104-108 8631809-5 1996 In transient transfection assays, both multicopy NFAT- and IL-2 promoter-beta-galactosidase reporter gene constructs could be activated by phorbol 12-myristate 13-acetate (PMA)/alpha-CD28 stimulation, and this activation was resistant to CsA. Tetradecanoylphorbol Acetate 139-170 interleukin 2 Homo sapiens 59-63 8631809-5 1996 In transient transfection assays, both multicopy NFAT- and IL-2 promoter-beta-galactosidase reporter gene constructs could be activated by phorbol 12-myristate 13-acetate (PMA)/alpha-CD28 stimulation, and this activation was resistant to CsA. Tetradecanoylphorbol Acetate 172-175 interleukin 2 Homo sapiens 59-63 8543831-7 1996 In addition, in TPA-activated Molt-4 T cells, an increased expression of IL-2 and IL-2-specific transcripts was detected. Tetradecanoylphorbol Acetate 16-19 interleukin 2 Homo sapiens 82-86 8543831-7 1996 In addition, in TPA-activated Molt-4 T cells, an increased expression of IL-2 and IL-2-specific transcripts was detected. Tetradecanoylphorbol Acetate 16-19 interleukin 2 Homo sapiens 73-77 7594459-3 1995 These activated T cells produced IL-2, IL-4, IL-5, and IFN-gamma upon stimulation with PMA and ionomycin. Tetradecanoylphorbol Acetate 87-90 interleukin 2 Homo sapiens 33-37 8595373-14 1995 In addition the effect of CD28 co-stimulation on IL-2 mRNA stabilisation was demonstrated by the maintenance of a high frequency of IL-2 expressing CD4 T cells and an elevated level of mRNA per cell for prolonged period after PMA+Io stimulation. Tetradecanoylphorbol Acetate 226-229 interleukin 2 Homo sapiens 49-53 8547073-4 1995 Our tests showed that IL-2 was produced when leukaemic B cells were stimulated with phorbol myristate acetate, ionomycin and lipopolysaccharide. Tetradecanoylphorbol Acetate 84-109 interleukin 2 Homo sapiens 22-26 8550072-5 1995 With adult purified T cells, high levels of IL-10 and IL-4 were measured following CD3 plus CD28 stimulation, and the amounts of both T-helper type-2 (Th2) cytokines decreased following the addition of phorbol myristate acetate (PMA), whereas the synthesis of the Th1 cytokines IL-2 and IFN-gamma was enhanced. Tetradecanoylphorbol Acetate 202-227 interleukin 2 Homo sapiens 278-282 7585980-6 1995 Similar concentrations of herbimycin A also inhibited the increase in IL-2 mRNA expression and cell proliferation in T cell cultures after IOR-T1/RAM Ig and TPA treatment. Tetradecanoylphorbol Acetate 157-160 interleukin 2 Homo sapiens 70-74 7554401-5 1995 Importantly, the levels of mRNA encoding c-myc, IL-2R alpha, IL-2 and IFN-gamma were markedly decreased in patient lymphocytes stimulated with PMA+ionomycin as compared to control lymphocytes. Tetradecanoylphorbol Acetate 143-146 interleukin 2 Homo sapiens 48-52 7768542-2 1995 These unfractionated thymocytes could be selectively expanded in vitro by stimulation with 12-O-tetradecanoylphorbol 13-acetate (TPA) and PHA in the presence of IL-2. Tetradecanoylphorbol Acetate 129-132 interleukin 2 Homo sapiens 161-165 7590880-5 1995 Activation of B cells [phorbol myristate acetate (PMA), surface immunoglobulin cross-linking alone or in the presence of interleukin-2 (IL-2)] induced CD44E (variable exon V8-10), R2 (VIO) and CD44 isoforms containing exons V6 and/or V7 (CD44 V6/V7). Tetradecanoylphorbol Acetate 23-48 interleukin 2 Homo sapiens 136-140 7664781-3 1995 In the present study we analyzed the control of IL-2 promoter activity in Epstein-Barr virus (EBV)-transformed B cell clones which are capable of secreting IL-2 at a low level after stimulation with phorbol 12-myristate 13-acetate and the Ca2+ ionophore ionomycin. Tetradecanoylphorbol Acetate 199-230 interleukin 2 Homo sapiens 48-52 7664781-3 1995 In the present study we analyzed the control of IL-2 promoter activity in Epstein-Barr virus (EBV)-transformed B cell clones which are capable of secreting IL-2 at a low level after stimulation with phorbol 12-myristate 13-acetate and the Ca2+ ionophore ionomycin. Tetradecanoylphorbol Acetate 199-230 interleukin 2 Homo sapiens 156-160 7539752-5 1995 Lower IL-2 expression (detected only as mRNA synthesis) was also induced in the cultured B lymphocytes after incubation with cross-linking anti-IgM antibodies instead of PMA plus ionomycin. Tetradecanoylphorbol Acetate 170-173 interleukin 2 Homo sapiens 6-10 7869038-7 1995 Cotransfection of gamma B*CaM-K with the IL-2 promoter construct downregulated its transcription in response to stimulation with ionomycin and phorbol myristate acetate (PMA). Tetradecanoylphorbol Acetate 170-173 interleukin 2 Homo sapiens 41-45 7851022-13 1995 Stimulation with phytohaemagglutinin (PHA) and phorbol myristate acetate (PMA) induced most TCC to produce higher amounts of TNF-alpha, IL-2 and IL-6. Tetradecanoylphorbol Acetate 47-72 interleukin 2 Homo sapiens 136-140 7851022-13 1995 Stimulation with phytohaemagglutinin (PHA) and phorbol myristate acetate (PMA) induced most TCC to produce higher amounts of TNF-alpha, IL-2 and IL-6. Tetradecanoylphorbol Acetate 74-77 interleukin 2 Homo sapiens 136-140 7622191-2 1995 TG plus phorbol myristate acetate (PMA) but not TG alone induced IL-2 in Jurkat T cells, suggesting that TG had no effect on protein kinase C (PKC). Tetradecanoylphorbol Acetate 8-33 interleukin 2 Homo sapiens 65-69 7622191-2 1995 TG plus phorbol myristate acetate (PMA) but not TG alone induced IL-2 in Jurkat T cells, suggesting that TG had no effect on protein kinase C (PKC). Tetradecanoylphorbol Acetate 35-38 interleukin 2 Homo sapiens 65-69 7606799-4 1995 Phorbol ester (PMA), a direct activator of PKC, provoked LHRH production and cell surface expression of CD69 and IL-2R molecules by T cells, but not IL-2 synthesis. Tetradecanoylphorbol Acetate 15-18 interleukin 2 Homo sapiens 113-117 7606799-5 1995 The synthesis of IL-2 by T cells required costimulation with PMA and ionomycin, a Ca2+ ionophore. Tetradecanoylphorbol Acetate 61-64 interleukin 2 Homo sapiens 17-21 7543050-5 1995 For splenocytes and LNL from flight (FLT) animals, IL-2 production decreased in response to the T cell receptor-independent mitogen 12-O-tetradecanoylphorbol-13-acetate plus ionomycin, but was not affected by stimulation with the T cell receptor-dependent mitogens Concanavalin A or phytohemagglutinin. Tetradecanoylphorbol Acetate 132-168 interleukin 2 Homo sapiens 51-55 7706710-10 1995 Conversely, calcium-independent anti-CD28 Ab and PMA-induced IL-2 production was resistant. Tetradecanoylphorbol Acetate 49-52 interleukin 2 Homo sapiens 61-65 7869038-7 1995 Cotransfection of gamma B*CaM-K with the IL-2 promoter construct downregulated its transcription in response to stimulation with ionomycin and phorbol myristate acetate (PMA). Tetradecanoylphorbol Acetate 143-168 interleukin 2 Homo sapiens 41-45 8574146-3 1995 In contrast, anti-CD3 with PMA gives a more vigorous IL-2 response than with anti-CD28, ie, 37.3 ng/ml compared to 12.3 ng/ml for controls and 28.5 ng/ml versus 15.1 ng/ml for HIV+ cells, respectively. Tetradecanoylphorbol Acetate 27-30 interleukin 2 Homo sapiens 53-57 7879050-0 1995 Effect of rapamycin on the in vitro release of soluble interleukin-2 receptor by phytohemagglutinin, phorbol myristate acetate, and ionomycin-activated peripheral blood mononuclear cells. Tetradecanoylphorbol Acetate 101-126 interleukin 2 Homo sapiens 55-68 7983701-7 1995 Selected transfected clones produced low levels of IFN A (IFNA) constitutively, and their abilities to express interleukin-2 and interleukin-2 receptor upon stimulation with phytohemagglutinin and phorbol myristate acetate were retained. Tetradecanoylphorbol Acetate 197-222 interleukin 2 Homo sapiens 111-124 7983701-7 1995 Selected transfected clones produced low levels of IFN A (IFNA) constitutively, and their abilities to express interleukin-2 and interleukin-2 receptor upon stimulation with phytohemagglutinin and phorbol myristate acetate were retained. Tetradecanoylphorbol Acetate 197-222 interleukin 2 Homo sapiens 129-142 7983381-5 1994 Jurkat T lymphocytes were stimulated with ionomycin + phorbol myristate acetate to produce interleukin-2 (IL-2) mRNA in vitro overnight. Tetradecanoylphorbol Acetate 54-79 interleukin 2 Homo sapiens 91-104 7983381-5 1994 Jurkat T lymphocytes were stimulated with ionomycin + phorbol myristate acetate to produce interleukin-2 (IL-2) mRNA in vitro overnight. Tetradecanoylphorbol Acetate 54-79 interleukin 2 Homo sapiens 106-110 7948741-4 1994 Following phytohemagglutinin (PHA)/4 beta-phorbol 12-myristate 13 acetate (TPA) stimulation, a 14 kDa molecule could be visualized in Western blots by means of two monoclonal anti-IL-2 antibodies possessing different epitope specificities. Tetradecanoylphorbol Acetate 37-73 interleukin 2 Homo sapiens 180-184 8087865-7 1994 In contrast, PMA-induced IL-2-independent proliferation of human T cells appears to be dependent on the continuous presence of cPKC (that may mediate some critical events at late stages of the response) and, therefore, is inhibited by bryostatin, that induces a rapid degradation of potentially active cPKC. Tetradecanoylphorbol Acetate 13-16 interleukin 2 Homo sapiens 25-29 7931079-11 1994 Therefore, we propose that the TPA/calcium-activated AP-1/OAP element is the main target of positive or negative regulatory signals influencing the IL-2 octamer motif, through synergism with Oct-2 and antagonism by RAR. Tetradecanoylphorbol Acetate 31-34 interleukin 2 Homo sapiens 148-152 7915976-2 1994 Both peripheral blood mononuclear cells and IL-2-dependent T cell lines derived from the patient showed a severe selective T cell activation impairment via CD2, CD3 and CD43; however, this defect was reversible with the addition of either IL-2, or phorbol myristate acetate (PMA) or anti-CD28 antibodies. Tetradecanoylphorbol Acetate 248-273 interleukin 2 Homo sapiens 44-48 7718953-0 1994 Impaired interleukin-2 production in active ulcerative colitis is reversed by calcium ionophore plus phorbol myristate acetate and related to altered intracellular Ca2+ responses. Tetradecanoylphorbol Acetate 101-126 interleukin 2 Homo sapiens 9-22 7718953-3 1994 Depressed IL-2 production in active UC was not reversed by the addition of anti-CD3 monoclonal antibody plus phorbol myristate acetate (PMA), but was largely reversed by adding calcium ionophore plus PMA. Tetradecanoylphorbol Acetate 136-139 interleukin 2 Homo sapiens 10-14 7718953-3 1994 Depressed IL-2 production in active UC was not reversed by the addition of anti-CD3 monoclonal antibody plus phorbol myristate acetate (PMA), but was largely reversed by adding calcium ionophore plus PMA. Tetradecanoylphorbol Acetate 200-203 interleukin 2 Homo sapiens 10-14 7931079-1 1994 The differentiating agent retinoic acid (RA) has been previously reported to interfere with 12-O-tetradecanoyl-phorbol-13-acetate (TPA)/Ca2+-induced signals for the regulation of the -96 to -66-bp octamer motif found in the enhancer for the interleukin (IL)-2 gene, which encodes a major T lymphocyte growth factor. Tetradecanoylphorbol Acetate 92-129 interleukin 2 Homo sapiens 241-259 7931079-1 1994 The differentiating agent retinoic acid (RA) has been previously reported to interfere with 12-O-tetradecanoyl-phorbol-13-acetate (TPA)/Ca2+-induced signals for the regulation of the -96 to -66-bp octamer motif found in the enhancer for the interleukin (IL)-2 gene, which encodes a major T lymphocyte growth factor. Tetradecanoylphorbol Acetate 131-134 interleukin 2 Homo sapiens 241-259 7931079-2 1994 The IL-2 octamer motif is a composite cis-element which binds Oct-1 and Oct-2 as well as a TPA/Ca2+-inducible nuclear factor, previously termed octamer-associated protein (OAP40). Tetradecanoylphorbol Acetate 91-94 interleukin 2 Homo sapiens 4-8 7931079-3 1994 We show here that Oct-2, despite the presence of an active transcriptional activation domain, requires TPA/Ca2+-induced signals to strongly transactivate the IL-2 octamer motif in Jurkat T cells. Tetradecanoylphorbol Acetate 103-106 interleukin 2 Homo sapiens 158-162 7915976-2 1994 Both peripheral blood mononuclear cells and IL-2-dependent T cell lines derived from the patient showed a severe selective T cell activation impairment via CD2, CD3 and CD43; however, this defect was reversible with the addition of either IL-2, or phorbol myristate acetate (PMA) or anti-CD28 antibodies. Tetradecanoylphorbol Acetate 275-278 interleukin 2 Homo sapiens 44-48 7948741-4 1994 Following phytohemagglutinin (PHA)/4 beta-phorbol 12-myristate 13 acetate (TPA) stimulation, a 14 kDa molecule could be visualized in Western blots by means of two monoclonal anti-IL-2 antibodies possessing different epitope specificities. Tetradecanoylphorbol Acetate 75-78 interleukin 2 Homo sapiens 180-184 8207793-3 1994 Transient expression of HIV-1 Tat induced a five- to eightfold increase in IL-2 promoter activity in Jurkat T cells stimulated with phytohemagglutinin and phorbol myristate acetate. Tetradecanoylphorbol Acetate 155-180 interleukin 2 Homo sapiens 75-79 8205621-1 1994 T lymphocyte activation and interleukin-2 (IL-2) production require at least two signals, generated by phorbol ester (TPA) and Ca2+ ionophore or costimulation of the T cell receptor (TCR) and the CD28 auxiliary receptor. Tetradecanoylphorbol Acetate 118-121 interleukin 2 Homo sapiens 28-41 7517981-5 1994 In regard to FK-506, we found that 1) FK-506 completely blocked the production of IL-2; 2) exogeneous IL-2 consistently restored the FK-506-induced inhibition; 3) FK-506 affected the phorbol myristate acetate-induced IL-2 responsiveness very little, if any; and 4) the significant suppression was observed only when FK-506 was added within 24 h after the initiation of culture. Tetradecanoylphorbol Acetate 183-208 interleukin 2 Homo sapiens 102-106 7517981-5 1994 In regard to FK-506, we found that 1) FK-506 completely blocked the production of IL-2; 2) exogeneous IL-2 consistently restored the FK-506-induced inhibition; 3) FK-506 affected the phorbol myristate acetate-induced IL-2 responsiveness very little, if any; and 4) the significant suppression was observed only when FK-506 was added within 24 h after the initiation of culture. Tetradecanoylphorbol Acetate 183-208 interleukin 2 Homo sapiens 102-106 8205621-1 1994 T lymphocyte activation and interleukin-2 (IL-2) production require at least two signals, generated by phorbol ester (TPA) and Ca2+ ionophore or costimulation of the T cell receptor (TCR) and the CD28 auxiliary receptor. Tetradecanoylphorbol Acetate 118-121 interleukin 2 Homo sapiens 43-47 7905497-4 1994 We report that prolonged exposure of immunologically naive and unstimulated human neonatal CD4 T cells to IL-4 or to IL-4 plus either IL-2 or IL-12 markedly affects their cytokine production on primary stimulation with PMA and ionomycin. Tetradecanoylphorbol Acetate 219-222 interleukin 2 Homo sapiens 134-138 7917514-2 1994 Secretion of IL-2 and TNF-alpha, surface expression of IL-2R, and DNA-binding activity of NF-kappa B and AP-1 (Fos/Jun) complex in response to phorbol myristate acetate, TNF-alpha, or immobilized antibodies to CD3 were monitored. Tetradecanoylphorbol Acetate 143-168 interleukin 2 Homo sapiens 13-17 7909823-6 1994 Primed cells are enriched in CD45R0hi and CD31- cells, and upon stimulation with PMA+ ionomycin they release significant amounts of IL-2, IFN-gamma, IL-4, IL-5, and IL-10. Tetradecanoylphorbol Acetate 81-84 interleukin 2 Homo sapiens 132-136 8071057-5 1994 Jurkat cells were used as a test model for TH1-type T-cells and were stimulated for IL-2 release with a combination of phytohemagglutinin and phorbol myristate acetate. Tetradecanoylphorbol Acetate 142-167 interleukin 2 Homo sapiens 84-88 8149968-5 1994 Interestingly, addition of either recombinant interleukin (IL)-2 or phorbol 12-myristate 13-acetate to the cell culture was able to completely restore impaired anti-CD3-induced proliferation in diabetic T cells, suggesting the presence of a defect through the TcR/CD3 pathway, located upstream of protein kinase C (PKC) activation and resulting in low IL-2 production and proliferation. Tetradecanoylphorbol Acetate 68-99 interleukin 2 Homo sapiens 352-356 7517692-6 1994 Secretion of interleukin-2 (IL-2) into the culture media was also detected after stimulation by PHA or TPA, but not in unstimulated cells. Tetradecanoylphorbol Acetate 103-106 interleukin 2 Homo sapiens 13-26 7517692-6 1994 Secretion of interleukin-2 (IL-2) into the culture media was also detected after stimulation by PHA or TPA, but not in unstimulated cells. Tetradecanoylphorbol Acetate 103-106 interleukin 2 Homo sapiens 28-32 7905497-7 1994 In response to primary stimulation with PMA and ionomycin, cells primed with IL-4 + IL-2 produce IL-4, IL-5, and IL-10 and the same levels of IL-2 and IFN-gamma as IL-4-primed cells. Tetradecanoylphorbol Acetate 40-43 interleukin 2 Homo sapiens 84-88 7905497-7 1994 In response to primary stimulation with PMA and ionomycin, cells primed with IL-4 + IL-2 produce IL-4, IL-5, and IL-10 and the same levels of IL-2 and IFN-gamma as IL-4-primed cells. Tetradecanoylphorbol Acetate 40-43 interleukin 2 Homo sapiens 142-146 8003628-2 1994 As incubation of lymphocytes with the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) prior to mitogenic stimulation results in decreased levels of IL-2 mRNA, we asked if IL-2 mRNA stability was affected. Tetradecanoylphorbol Acetate 52-88 interleukin 2 Homo sapiens 157-161 8003628-2 1994 As incubation of lymphocytes with the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) prior to mitogenic stimulation results in decreased levels of IL-2 mRNA, we asked if IL-2 mRNA stability was affected. Tetradecanoylphorbol Acetate 90-93 interleukin 2 Homo sapiens 157-161 8003628-3 1994 We found that in TPA-treated cells, IL-2 mRNA was degraded more rapidly than in untreated ones whether the mitogenic stimulus was Concanavalin A (Con A), Con A plus TPA, or TPA plus ionomycin. Tetradecanoylphorbol Acetate 17-20 interleukin 2 Homo sapiens 36-40 8003628-3 1994 We found that in TPA-treated cells, IL-2 mRNA was degraded more rapidly than in untreated ones whether the mitogenic stimulus was Concanavalin A (Con A), Con A plus TPA, or TPA plus ionomycin. Tetradecanoylphorbol Acetate 165-168 interleukin 2 Homo sapiens 36-40 8003628-3 1994 We found that in TPA-treated cells, IL-2 mRNA was degraded more rapidly than in untreated ones whether the mitogenic stimulus was Concanavalin A (Con A), Con A plus TPA, or TPA plus ionomycin. Tetradecanoylphorbol Acetate 165-168 interleukin 2 Homo sapiens 36-40 8003628-5 1994 In contrast, when TPA was included as a co-mitogen, i.e. added at the same time as the mitogen, the IL-2 mRNA levels and stability significantly increased. Tetradecanoylphorbol Acetate 18-21 interleukin 2 Homo sapiens 100-104 8003628-6 1994 Compared to the levels found in Con A stimulated cells, TPA plus Con A increased IL-2 mRNA levels by as much as 20-fold and the half-life by 5-fold. Tetradecanoylphorbol Acetate 56-59 interleukin 2 Homo sapiens 81-85 8262983-5 1993 Furthermore, normal and active Fyn stimulated transcription from the IL-2 gene promoter when transfected cells were stimulated by concanavalin A plus 12-O-tetradecanoylphorbol-13-acetate. Tetradecanoylphorbol Acetate 150-186 interleukin 2 Homo sapiens 69-73 8186461-1 1994 Granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-2 (IL-2) are produced by stimulation with phorbol-12-myristate acetate (PMA) and calcium ionophore (A23187) in human T cell leukemia Jurkat cells. Tetradecanoylphorbol Acetate 146-149 interleukin 2 Homo sapiens 62-75 8186461-1 1994 Granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-2 (IL-2) are produced by stimulation with phorbol-12-myristate acetate (PMA) and calcium ionophore (A23187) in human T cell leukemia Jurkat cells. Tetradecanoylphorbol Acetate 146-149 interleukin 2 Homo sapiens 77-81 8186461-6 1994 We also found that the active CN partially replaces calcium ionophore in synergy with PMA to induce expression of endogenous GM-CSF and IL-2. Tetradecanoylphorbol Acetate 86-89 interleukin 2 Homo sapiens 136-140 8223876-2 1993 The region extending from -317 to +47 relative to the initiation site of IL-2 gene transcription was shown to contain sequences able to respond to CD69 cross-linking, by enhancing by about 100% a phorbol 12-myristate 13-acetate (PMA)-plus-ionomycin stimulation of CAT activity. Tetradecanoylphorbol Acetate 196-227 interleukin 2 Homo sapiens 73-77 8258145-6 1993 H47 mAb also enhanced PMA-induced interleukin-2 receptor (IL-2R) expression and IL-2 synthesis, but did not induce a change in intracellular free calcium ([Ca2+]i) of T cells. Tetradecanoylphorbol Acetate 22-25 interleukin 2 Homo sapiens 58-62 8223876-2 1993 The region extending from -317 to +47 relative to the initiation site of IL-2 gene transcription was shown to contain sequences able to respond to CD69 cross-linking, by enhancing by about 100% a phorbol 12-myristate 13-acetate (PMA)-plus-ionomycin stimulation of CAT activity. Tetradecanoylphorbol Acetate 229-232 interleukin 2 Homo sapiens 73-77 8210444-6 1993 IL-2 enhanced the stimulatory actions of interleukin-1 (IL-1), epidermal growth factor (EGF), ionomycin, and phorbol 12-myristate 13-acetate (PMA) on PGE2 production by decidual cells. Tetradecanoylphorbol Acetate 109-140 interleukin 2 Homo sapiens 0-4 7903276-4 1993 In the presence of phorbol myristate acetate (PMA), large amounts of IL-2 were induced by both anti-CD3 and anti-CD2 stimulation, which was accompanied by strong concurrent tyrosine phosphorylation of the 42,000 MW ERK and a 100,000 MW protein. Tetradecanoylphorbol Acetate 19-44 interleukin 2 Homo sapiens 69-73 7903276-4 1993 In the presence of phorbol myristate acetate (PMA), large amounts of IL-2 were induced by both anti-CD3 and anti-CD2 stimulation, which was accompanied by strong concurrent tyrosine phosphorylation of the 42,000 MW ERK and a 100,000 MW protein. Tetradecanoylphorbol Acetate 46-49 interleukin 2 Homo sapiens 69-73 8406577-3 1993 The augmentation by GL of IL-2 production was also found in spleen cells stimulated with A23187 plus phorbol 12-myristate 13-acetate (PMA), suggesting that GL primarily affects some post-receptor stage of the signal transduction. Tetradecanoylphorbol Acetate 101-132 interleukin 2 Homo sapiens 26-30 8406577-3 1993 The augmentation by GL of IL-2 production was also found in spleen cells stimulated with A23187 plus phorbol 12-myristate 13-acetate (PMA), suggesting that GL primarily affects some post-receptor stage of the signal transduction. Tetradecanoylphorbol Acetate 134-137 interleukin 2 Homo sapiens 26-30 8233727-11 1993 Considering the low proportion of lymphocytes, stimulation with phorbol myristate acetate in combination with ionomycin resulted in considerable production of the following lymphokines: IL-2, IL-3, IL-4, IL-10, interferon-gamma, tumor necrosis factor-alpha. Tetradecanoylphorbol Acetate 64-89 interleukin 2 Homo sapiens 186-190 8210444-6 1993 IL-2 enhanced the stimulatory actions of interleukin-1 (IL-1), epidermal growth factor (EGF), ionomycin, and phorbol 12-myristate 13-acetate (PMA) on PGE2 production by decidual cells. Tetradecanoylphorbol Acetate 142-145 interleukin 2 Homo sapiens 0-4 7901231-8 1993 Furthermore, stimulation of both CVI patient and normal CD4+ T cells with either ionomycin+phorbol myristate acetate or a combination of immobilized anti-CD3 antibody plus anti-CD28 antibody resulted in a 50-fold increase in IL-2 production compared to stimulation with immobilized anti-CD3 antibody alone, and, under these conditions, CVI and normal CD4+ T cells produced equivalent amounts of IL-2. Tetradecanoylphorbol Acetate 91-116 interleukin 2 Homo sapiens 225-229 8324074-4 1993 In this study, we examined the effect of cytochalasin B (CB) plus 12-O-tetradecanoylphorbol-13-acetate (TPA) on expression of IL-2 and IL-2R. Tetradecanoylphorbol Acetate 66-102 interleukin 2 Homo sapiens 126-130 8324074-4 1993 In this study, we examined the effect of cytochalasin B (CB) plus 12-O-tetradecanoylphorbol-13-acetate (TPA) on expression of IL-2 and IL-2R. Tetradecanoylphorbol Acetate 104-107 interleukin 2 Homo sapiens 126-130 8324074-8 1993 In order to determine the percentage of cells that incorporated tritiated thymidine ([3H]dT) in the presence of IL-2 after treatment with CB plus TPA, autoradiography was carried out. Tetradecanoylphorbol Acetate 146-149 interleukin 2 Homo sapiens 112-116 8141269-5 1994 Stimulation with ionomycin plus phorbol 12-myristate 13-acetate leads to intracellular alkalinization within 90 min, reaching the more alkaline steady-state value of 7.25 within 7-10 h. Proliferation, but not viability, of lymphocytes is dependent on extracellular pH in the range of 6.4-8.0, and this dependence is not due to limiting interleukin-2 elaboration. Tetradecanoylphorbol Acetate 32-63 interleukin 2 Homo sapiens 336-349 8100719-4 1993 When stimulated with anti-CD3 and phorbol 12-myristate 13-acetate, purified patient CD8+ T cells exhibited significantly decreased proliferation, c-myc expression, and interleukin-2 (IL-2) production compared with that of normal CD8+ T cells. Tetradecanoylphorbol Acetate 34-65 interleukin 2 Homo sapiens 168-181 8100719-4 1993 When stimulated with anti-CD3 and phorbol 12-myristate 13-acetate, purified patient CD8+ T cells exhibited significantly decreased proliferation, c-myc expression, and interleukin-2 (IL-2) production compared with that of normal CD8+ T cells. Tetradecanoylphorbol Acetate 34-65 interleukin 2 Homo sapiens 183-187 8384561-2 1993 As detected by reverse transcriptase-polymerase chain reaction, IL-2 mRNA was expressed only after stimulation with the combination of phorbol 12-myristate 13-acetate (PMA) plus ionomycin. Tetradecanoylphorbol Acetate 135-166 interleukin 2 Homo sapiens 64-68 8384561-2 1993 As detected by reverse transcriptase-polymerase chain reaction, IL-2 mRNA was expressed only after stimulation with the combination of phorbol 12-myristate 13-acetate (PMA) plus ionomycin. Tetradecanoylphorbol Acetate 168-171 interleukin 2 Homo sapiens 64-68 8419187-4 1993 After stimulation with phorbol 12-myristate 13-acetate (PMA) and calcium ionophore A23187, they produced higher levels of IL-4 (306 vs. 55 +/- 4 pg/ml) and IL-5 (2900 vs. 213 +/- 72 pg/ml) and lower levels of IL-2 (17 vs. 63 +/- 17 IU/ml) and interferon-gamma (IFN-gamma) (16 vs. 299 +/- 70 IU/ml) than controls CD4+ CD45R0+ cells. Tetradecanoylphorbol Acetate 23-54 interleukin 2 Homo sapiens 209-213 8423347-7 1993 T cell lines generated against Tct proliferated in response to parasite lysate only in the presence of autologous APC and produced IL-2, IL-6, and IFN-gamma but not IL-4 in response to PMA plus ionomycin. Tetradecanoylphorbol Acetate 185-188 interleukin 2 Homo sapiens 131-135 8425229-6 1993 When a combination of PHA and 12-O-tetradecanoyl-13-O-acetyl phorbol (TPA) was used, a small increase in IL-2 production was observed only in the presence of vinblastine (10 microM). Tetradecanoylphorbol Acetate 70-73 interleukin 2 Homo sapiens 105-109 8419187-4 1993 After stimulation with phorbol 12-myristate 13-acetate (PMA) and calcium ionophore A23187, they produced higher levels of IL-4 (306 vs. 55 +/- 4 pg/ml) and IL-5 (2900 vs. 213 +/- 72 pg/ml) and lower levels of IL-2 (17 vs. 63 +/- 17 IU/ml) and interferon-gamma (IFN-gamma) (16 vs. 299 +/- 70 IU/ml) than controls CD4+ CD45R0+ cells. Tetradecanoylphorbol Acetate 56-59 interleukin 2 Homo sapiens 209-213 8320080-4 1993 A phorbol-ester (PMA), on the other hand, enhanced only slightly the proportion of PE-IL-2 binding cells. Tetradecanoylphorbol Acetate 17-20 interleukin 2 Homo sapiens 86-90 1331177-5 1992 In human corticotrophic adenoma cells, basal IL-2 mRNA expression as well as IL-2 secretion were further stimulated by phorbol myristate acetate. Tetradecanoylphorbol Acetate 119-144 interleukin 2 Homo sapiens 45-49 8443122-1 1993 Few known genes (IL-2, members of the IL-8 family, interferon-gamma) are induced in T cells only through the combined effect of phorbol myristic acetate (PMA) and a Ca(2+)-ionophore, and expression of only these genes can be fully suppressed by Cyclosporin A (CyA). Tetradecanoylphorbol Acetate 154-157 interleukin 2 Homo sapiens 17-21 18475504-4 1993 IL-2 had no effect on neutrophil migration, phagocytosis, deoxyglucose uptake or degranulation, ionocytes demonstrated a greater sensitivity to IL-2 with suppression of monocyte adherence, random and stimulated migration, glucose uptake and hexose monophosphate shunt activity, even after addition of phorbol myristate acetate. Tetradecanoylphorbol Acetate 301-326 interleukin 2 Homo sapiens 144-148 1331177-5 1992 In human corticotrophic adenoma cells, basal IL-2 mRNA expression as well as IL-2 secretion were further stimulated by phorbol myristate acetate. Tetradecanoylphorbol Acetate 119-144 interleukin 2 Homo sapiens 77-81 1740658-2 1992 We report that the Dex-dependent downregulation of 12-O-tetradecanoyl-phorbol-13-acetate (TPA) and calcium ionophore-induced activity of the IL-2 enhancer are mediated by glucocorticoid receptor (GR) via a process that requires intact NH2- and COOH-terminal and DNA-binding domains. Tetradecanoylphorbol Acetate 90-93 interleukin 2 Homo sapiens 141-145 1394441-3 1992 After stimulation with phytohemagglutinin (PHA) and phorbol myristate acetate (PMA), Tat transfectants with high Tat expression showed diminished expression of interleukin-2 (IL-2) and the interleukin-2 receptor alpha chain (IL-2R) when compared to untransfected Jurkat cells or Jurkat cell lines transfected with the parent control plasmid. Tetradecanoylphorbol Acetate 52-77 interleukin 2 Homo sapiens 160-173 1394441-3 1992 After stimulation with phytohemagglutinin (PHA) and phorbol myristate acetate (PMA), Tat transfectants with high Tat expression showed diminished expression of interleukin-2 (IL-2) and the interleukin-2 receptor alpha chain (IL-2R) when compared to untransfected Jurkat cells or Jurkat cell lines transfected with the parent control plasmid. Tetradecanoylphorbol Acetate 52-77 interleukin 2 Homo sapiens 175-179 1394441-3 1992 After stimulation with phytohemagglutinin (PHA) and phorbol myristate acetate (PMA), Tat transfectants with high Tat expression showed diminished expression of interleukin-2 (IL-2) and the interleukin-2 receptor alpha chain (IL-2R) when compared to untransfected Jurkat cells or Jurkat cell lines transfected with the parent control plasmid. Tetradecanoylphorbol Acetate 52-77 interleukin 2 Homo sapiens 189-202 1322203-7 1992 We now report that PA682BM-1 can be triggered by the protein kinase C (PKC) activators, phorbol 12-myristate 13-acetate (PMA) and (-)Indolactam-v, to secrete IFN gamma, whereas JLP(c) cells spontaneously produce low levels of IFN gamma that can be enhanced by PKC activators and interleukin-2 (IL-2). Tetradecanoylphorbol Acetate 88-119 interleukin 2 Homo sapiens 279-292 1322203-7 1992 We now report that PA682BM-1 can be triggered by the protein kinase C (PKC) activators, phorbol 12-myristate 13-acetate (PMA) and (-)Indolactam-v, to secrete IFN gamma, whereas JLP(c) cells spontaneously produce low levels of IFN gamma that can be enhanced by PKC activators and interleukin-2 (IL-2). Tetradecanoylphorbol Acetate 88-119 interleukin 2 Homo sapiens 294-298 1322203-7 1992 We now report that PA682BM-1 can be triggered by the protein kinase C (PKC) activators, phorbol 12-myristate 13-acetate (PMA) and (-)Indolactam-v, to secrete IFN gamma, whereas JLP(c) cells spontaneously produce low levels of IFN gamma that can be enhanced by PKC activators and interleukin-2 (IL-2). Tetradecanoylphorbol Acetate 121-124 interleukin 2 Homo sapiens 279-292 1322203-7 1992 We now report that PA682BM-1 can be triggered by the protein kinase C (PKC) activators, phorbol 12-myristate 13-acetate (PMA) and (-)Indolactam-v, to secrete IFN gamma, whereas JLP(c) cells spontaneously produce low levels of IFN gamma that can be enhanced by PKC activators and interleukin-2 (IL-2). Tetradecanoylphorbol Acetate 121-124 interleukin 2 Homo sapiens 294-298 1417980-10 1992 We suggest that the inhibition of thymocyte proliferation by staurosporine results from inhibition of both protein kinase C and tyrosine kinase: the augmentation of the response to A23187 and TPA results from inhibition of protein kinase C. Inhibition of signal transduction as well as inhibition of IL-2-driven mitogenesis result from inhibition of tyrosine kinase. Tetradecanoylphorbol Acetate 192-195 interleukin 2 Homo sapiens 300-304 1397084-0 1992 Cytochalasans and PMA induce IL-2 receptors on CD8+ lymphocytes. Tetradecanoylphorbol Acetate 18-21 interleukin 2 Homo sapiens 29-33 1398515-5 1992 Supernatants of TPA-stimulated Co cells contained the cytokines IL2, IL3, IL4 and IL8, whereas these cytokines were not detected in the supernatants of untreated cells. Tetradecanoylphorbol Acetate 16-19 interleukin 2 Homo sapiens 64-67 1380243-2 1992 Nuclear factors derived from thymocytes activated with phorbol myristate acetate and concanavalin A were tested for their ability to bind to a synthetic radiolabelled probe corresponding to the NF-AT region (-285 to -255) of the IL-2 gene. Tetradecanoylphorbol Acetate 55-80 interleukin 2 Homo sapiens 229-233 1588792-0 1992 Phorbol myristate acetate-induced expression of high-affinity interleukin 2 receptors and production of interleukin 2 by human acute lymphoblastic leukemia T cells. Tetradecanoylphorbol Acetate 0-25 interleukin 2 Homo sapiens 62-75 1588792-1 1992 The effect of phorbol myristate acetate (PMA) on the expression of interleukin 2 receptors (IL-2R), production of IL-2 and IL-2-dependent proliferation of acute lymphoblastic leukemia T cells (T-ALL cells) from 10 patients was studied. Tetradecanoylphorbol Acetate 41-44 interleukin 2 Homo sapiens 92-96 1588792-5 1992 We found that PMA induced the expression of both IL-2R alpha and IL-2R beta chains, as well as IL-2 production by T-ALL cells. Tetradecanoylphorbol Acetate 14-17 interleukin 2 Homo sapiens 49-53 1588792-8 1992 In two cases tested high-affinity IL-2R on PMA-treated T-ALL cells could internalize 125I-rIL-2 at 37 degrees C. PMA alone enhanced the spontaneous proliferation of T-ALL cells in three cases, whereas a clear synergy between IL-2 and PMA could be detected in three patients" cells. Tetradecanoylphorbol Acetate 43-46 interleukin 2 Homo sapiens 34-38 1531456-2 1992 IL-2 production was induced from these cells by pulsing them with mAb to CD3 and costimulating with phorbol myristate acetate (PMA). Tetradecanoylphorbol Acetate 100-125 interleukin 2 Homo sapiens 0-4 1531456-2 1992 IL-2 production was induced from these cells by pulsing them with mAb to CD3 and costimulating with phorbol myristate acetate (PMA). Tetradecanoylphorbol Acetate 127-130 interleukin 2 Homo sapiens 0-4 1531456-7 1992 By contrast, immobilized GaMIg was a potent stimulus for IL-2 production by T cells pulsed with anti-CD3 mAb and costimulated with PMA. Tetradecanoylphorbol Acetate 131-134 interleukin 2 Homo sapiens 57-61 1511462-5 1992 Phytohemagglutinin- and 12-O-tetradecanoylphorbol-13-acetate-induced production of interleukin-2 (IL-2) and NK-cell-mediated cytotoxic activity by peripheral blood mononuclear cells (PBMC) were also substantially decreased in the post-therapy groups. Tetradecanoylphorbol Acetate 24-60 interleukin 2 Homo sapiens 83-96 1730605-3 1992 Nuclear extracts from cells stimulated with phorbol 12-myristate 13-acetate and ionomycin, which activate protein kinase C and mimic physiological activation through the T-cell antigen receptor, transcribe an interleukin-2 (IL-2) enhancer (-326 to +24) template 5-fold more efficient than nuclear extracts from resting T-cells and severalfold more efficient than extracts from Jurkat cells treated with phorbol 12-myristate 13-acetate or ionomycin alone. Tetradecanoylphorbol Acetate 44-75 interleukin 2 Homo sapiens 209-222 1730605-3 1992 Nuclear extracts from cells stimulated with phorbol 12-myristate 13-acetate and ionomycin, which activate protein kinase C and mimic physiological activation through the T-cell antigen receptor, transcribe an interleukin-2 (IL-2) enhancer (-326 to +24) template 5-fold more efficient than nuclear extracts from resting T-cells and severalfold more efficient than extracts from Jurkat cells treated with phorbol 12-myristate 13-acetate or ionomycin alone. Tetradecanoylphorbol Acetate 44-75 interleukin 2 Homo sapiens 224-228 1730605-3 1992 Nuclear extracts from cells stimulated with phorbol 12-myristate 13-acetate and ionomycin, which activate protein kinase C and mimic physiological activation through the T-cell antigen receptor, transcribe an interleukin-2 (IL-2) enhancer (-326 to +24) template 5-fold more efficient than nuclear extracts from resting T-cells and severalfold more efficient than extracts from Jurkat cells treated with phorbol 12-myristate 13-acetate or ionomycin alone. Tetradecanoylphorbol Acetate 403-434 interleukin 2 Homo sapiens 209-222 1730605-3 1992 Nuclear extracts from cells stimulated with phorbol 12-myristate 13-acetate and ionomycin, which activate protein kinase C and mimic physiological activation through the T-cell antigen receptor, transcribe an interleukin-2 (IL-2) enhancer (-326 to +24) template 5-fold more efficient than nuclear extracts from resting T-cells and severalfold more efficient than extracts from Jurkat cells treated with phorbol 12-myristate 13-acetate or ionomycin alone. Tetradecanoylphorbol Acetate 403-434 interleukin 2 Homo sapiens 224-228 1370514-8 1992 Stimulation with Con A also induced very low or no measurable levels of IL-2 and IFN-gamma, whereas activation with TPA and the calcium ionophore A23187 resulted in the production of high levels of IL-4, IL-5, IL-2, and IFN-gamma. Tetradecanoylphorbol Acetate 116-119 interleukin 2 Homo sapiens 210-214 1371427-0 1992 Phorbol 12-myristate 13-acetate induces resistance of human melanoma cells to natural-killer- and lymphokine-activated-killer-mediated cytotoxicity. Tetradecanoylphorbol Acetate 0-31 interleukin 2 Homo sapiens 98-108 1511462-5 1992 Phytohemagglutinin- and 12-O-tetradecanoylphorbol-13-acetate-induced production of interleukin-2 (IL-2) and NK-cell-mediated cytotoxic activity by peripheral blood mononuclear cells (PBMC) were also substantially decreased in the post-therapy groups. Tetradecanoylphorbol Acetate 24-60 interleukin 2 Homo sapiens 98-102 1531763-2 1992 High in vitro growth ability was observed in response to recombinant human IL-2 (rIL-2) and human rIL-7, both in the absence of any co-mitogen and in combination with phorbol 12-myristate 13-acetate (PMA). Tetradecanoylphorbol Acetate 200-203 interleukin 2 Homo sapiens 75-79 1733630-5 1992 The IL-2R beta chain is constitutively expressed on freshly isolated thymocytes; this expression can be increased in thymocytes activated with Con A in combination with IL-2 or tetradecanoylphorbol 13-acetate (TPA). Tetradecanoylphorbol Acetate 210-213 interleukin 2 Homo sapiens 4-8 1371491-8 1992 When the cells were stimulated by phorbol ester (phorbol 12-myristate 13-acetate, PMA) plus calcium ionophore (ionomycin), FK506 and CsA inhibited, in a dose-dependent manner, the production of IL-2, IL-4, IL-5, IFN-gamma and TNF-alpha. Tetradecanoylphorbol Acetate 49-80 interleukin 2 Homo sapiens 194-198 1371491-8 1992 When the cells were stimulated by phorbol ester (phorbol 12-myristate 13-acetate, PMA) plus calcium ionophore (ionomycin), FK506 and CsA inhibited, in a dose-dependent manner, the production of IL-2, IL-4, IL-5, IFN-gamma and TNF-alpha. Tetradecanoylphorbol Acetate 82-85 interleukin 2 Homo sapiens 194-198 1836162-4 1991 Similarly, human colostrum inhibited the production of IL-2 by EL4 cells, a murine thymoma line, when stimulated with phorbol myristate acetate. Tetradecanoylphorbol Acetate 118-143 interleukin 2 Homo sapiens 55-59 1492121-5 1992 For most lymphokine genes, a combination of phorbol esters (phorbol 12-myristate 13 acetate, PMA) and calcium ionophores (A23187) is required for their maximal induction. Tetradecanoylphorbol Acetate 60-91 interleukin 2 Homo sapiens 9-19 1492121-5 1992 For most lymphokine genes, a combination of phorbol esters (phorbol 12-myristate 13 acetate, PMA) and calcium ionophores (A23187) is required for their maximal induction. Tetradecanoylphorbol Acetate 93-96 interleukin 2 Homo sapiens 9-19 1954393-6 1991 In contrast, lymphokine production in patients treated with immunosuppressive drugs (cyclosporine A, corticosteroids) was abnormal after stimulation with PMA and ionophore, as well as ConA. Tetradecanoylphorbol Acetate 154-157 interleukin 2 Homo sapiens 13-23 1718026-5 1991 By stimulation with phytohaemagglutinin (PHA) plus phorbol myristate acetate (PMA), three of the seven helper-type clones produced interleukin 2 (IL-2) in addition to IL-4. Tetradecanoylphorbol Acetate 51-76 interleukin 2 Homo sapiens 131-144 1919362-1 1991 Human T-cell cultures infected with human T-lymphotropic virus type I (HTLV-I) and interleukin-2 (IL-2)-dependent for their continuous growth were treated with N-methyl-N"-nitro-N-nitrosoguanidine (MNNG) and then maintained in the medium containing phorbol 12-myristate 13-acetate (TPA). Tetradecanoylphorbol Acetate 249-280 interleukin 2 Homo sapiens 98-102 1919362-1 1991 Human T-cell cultures infected with human T-lymphotropic virus type I (HTLV-I) and interleukin-2 (IL-2)-dependent for their continuous growth were treated with N-methyl-N"-nitro-N-nitrosoguanidine (MNNG) and then maintained in the medium containing phorbol 12-myristate 13-acetate (TPA). Tetradecanoylphorbol Acetate 282-285 interleukin 2 Homo sapiens 98-102 1679947-4 1991 The enhancing effect of lithium on IL-2 production showed some differences from that of tetradecanoylphorbol acetate (TPA) in the following aspects: (i) TPA could reverse the inhibitory effect of anti-CD2 monoclonal antibody on IL-2 production, whereas lithium could not; and (ii) lithium was unable to synergistically induce IL-2 production with anti-CD3 monoclonal antibody as TPA did. Tetradecanoylphorbol Acetate 153-156 interleukin 2 Homo sapiens 35-39 1679947-4 1991 The enhancing effect of lithium on IL-2 production showed some differences from that of tetradecanoylphorbol acetate (TPA) in the following aspects: (i) TPA could reverse the inhibitory effect of anti-CD2 monoclonal antibody on IL-2 production, whereas lithium could not; and (ii) lithium was unable to synergistically induce IL-2 production with anti-CD3 monoclonal antibody as TPA did. Tetradecanoylphorbol Acetate 153-156 interleukin 2 Homo sapiens 228-232 1679947-4 1991 The enhancing effect of lithium on IL-2 production showed some differences from that of tetradecanoylphorbol acetate (TPA) in the following aspects: (i) TPA could reverse the inhibitory effect of anti-CD2 monoclonal antibody on IL-2 production, whereas lithium could not; and (ii) lithium was unable to synergistically induce IL-2 production with anti-CD3 monoclonal antibody as TPA did. Tetradecanoylphorbol Acetate 153-156 interleukin 2 Homo sapiens 228-232 1679947-4 1991 The enhancing effect of lithium on IL-2 production showed some differences from that of tetradecanoylphorbol acetate (TPA) in the following aspects: (i) TPA could reverse the inhibitory effect of anti-CD2 monoclonal antibody on IL-2 production, whereas lithium could not; and (ii) lithium was unable to synergistically induce IL-2 production with anti-CD3 monoclonal antibody as TPA did. Tetradecanoylphorbol Acetate 153-156 interleukin 2 Homo sapiens 35-39 1679947-4 1991 The enhancing effect of lithium on IL-2 production showed some differences from that of tetradecanoylphorbol acetate (TPA) in the following aspects: (i) TPA could reverse the inhibitory effect of anti-CD2 monoclonal antibody on IL-2 production, whereas lithium could not; and (ii) lithium was unable to synergistically induce IL-2 production with anti-CD3 monoclonal antibody as TPA did. Tetradecanoylphorbol Acetate 153-156 interleukin 2 Homo sapiens 228-232 1679947-4 1991 The enhancing effect of lithium on IL-2 production showed some differences from that of tetradecanoylphorbol acetate (TPA) in the following aspects: (i) TPA could reverse the inhibitory effect of anti-CD2 monoclonal antibody on IL-2 production, whereas lithium could not; and (ii) lithium was unable to synergistically induce IL-2 production with anti-CD3 monoclonal antibody as TPA did. Tetradecanoylphorbol Acetate 153-156 interleukin 2 Homo sapiens 228-232 1718026-5 1991 By stimulation with phytohaemagglutinin (PHA) plus phorbol myristate acetate (PMA), three of the seven helper-type clones produced interleukin 2 (IL-2) in addition to IL-4. Tetradecanoylphorbol Acetate 51-76 interleukin 2 Homo sapiens 146-150 1718026-5 1991 By stimulation with phytohaemagglutinin (PHA) plus phorbol myristate acetate (PMA), three of the seven helper-type clones produced interleukin 2 (IL-2) in addition to IL-4. Tetradecanoylphorbol Acetate 78-81 interleukin 2 Homo sapiens 131-144 1718026-5 1991 By stimulation with phytohaemagglutinin (PHA) plus phorbol myristate acetate (PMA), three of the seven helper-type clones produced interleukin 2 (IL-2) in addition to IL-4. Tetradecanoylphorbol Acetate 78-81 interleukin 2 Homo sapiens 146-150 1830601-4 1991 For CD4-8- TCR-alpha beta early thymocytes that have not yet entered the selection process, PMA + ionomycin induced significant cell proliferation but little IL-2 production, in the absence of added IL-1. Tetradecanoylphorbol Acetate 92-95 interleukin 2 Homo sapiens 158-162 1761792-1 1991 T cell growth factors such as interleukin-2 (IL-2) and interleukin-4 (IL-4) act as potent comitogenic factors for purified human T cells in the presence of phorbol myristate acetate (PMA). Tetradecanoylphorbol Acetate 156-181 interleukin 2 Homo sapiens 30-43 1761792-1 1991 T cell growth factors such as interleukin-2 (IL-2) and interleukin-4 (IL-4) act as potent comitogenic factors for purified human T cells in the presence of phorbol myristate acetate (PMA). Tetradecanoylphorbol Acetate 156-181 interleukin 2 Homo sapiens 45-49 1761792-1 1991 T cell growth factors such as interleukin-2 (IL-2) and interleukin-4 (IL-4) act as potent comitogenic factors for purified human T cells in the presence of phorbol myristate acetate (PMA). Tetradecanoylphorbol Acetate 183-186 interleukin 2 Homo sapiens 30-43 1761792-1 1991 T cell growth factors such as interleukin-2 (IL-2) and interleukin-4 (IL-4) act as potent comitogenic factors for purified human T cells in the presence of phorbol myristate acetate (PMA). Tetradecanoylphorbol Acetate 183-186 interleukin 2 Homo sapiens 45-49 1761792-5 1991 PMA + IL-2 stimulation was more sensitive to the inhibitory effects of gangliosides (I50 = 77.2 microM) than PMA + IL-4 stimulation (I50 = 105.9 microM). Tetradecanoylphorbol Acetate 0-3 interleukin 2 Homo sapiens 6-10 1830601-10 1991 The lack of anti-TCR-induced IL-2 production by thymus CD4+8- T cells was not due to an intrinsic defect as high levels of IL-2 production was induced by PMA + ionomycin. Tetradecanoylphorbol Acetate 154-157 interleukin 2 Homo sapiens 123-127 1709824-7 1991 Although phorbol myristate acetate (PMA) greatly enhanced PHA-stimulated IL2 production by Jurkat cells. Tetradecanoylphorbol Acetate 9-34 interleukin 2 Homo sapiens 73-76 1709824-7 1991 Although phorbol myristate acetate (PMA) greatly enhanced PHA-stimulated IL2 production by Jurkat cells. Tetradecanoylphorbol Acetate 36-39 interleukin 2 Homo sapiens 73-76 1715317-2 1991 FK-506 and CsA were also potent inhibitors of A23187/PMA-stimulated IL-2 production by Jurkat and HuT-78 cells but had no effect on the response of mouse CTLL cells to IL-2. Tetradecanoylphorbol Acetate 53-56 interleukin 2 Homo sapiens 68-72 1832571-3 1991 IL2 secretion induced by phorbol 12-myristate 13-acetate (PMA) and the calcium ionophore A23187, which bypasses the breakdown of inositol phospholipids induced by the ligand-receptor interaction, was still suppressed by LO inhibitors which implies that these drugs also have an inhibitory effect on other target(s). Tetradecanoylphorbol Acetate 25-56 interleukin 2 Homo sapiens 0-3 1832571-3 1991 IL2 secretion induced by phorbol 12-myristate 13-acetate (PMA) and the calcium ionophore A23187, which bypasses the breakdown of inositol phospholipids induced by the ligand-receptor interaction, was still suppressed by LO inhibitors which implies that these drugs also have an inhibitory effect on other target(s). Tetradecanoylphorbol Acetate 58-61 interleukin 2 Homo sapiens 0-3 2052609-6 1991 Nef-1-expressing cells can produce IL-2 mRNA in response to a combination of PMA and ionomycin, although much less efficiently than the parental Jurkat cells or Nef-2-expressing cells. Tetradecanoylphorbol Acetate 77-80 interleukin 2 Homo sapiens 35-39 1903411-3 1991 In the present report we have studied various agents that, like TPA, act as partial or complete mitogens for G0 PBL and have determined their effect on phosphorylation of prosolin and on DNA synthesis in rapidly proliferating (IL-2-dependent) human PBL. Tetradecanoylphorbol Acetate 64-67 interleukin 2 Homo sapiens 227-231 1715761-0 1991 Negative regulation of interleukin-2 production in primary lymphocytes by 12-O-tetradecanoylphorbol-13-acetate. Tetradecanoylphorbol Acetate 74-110 interleukin 2 Homo sapiens 23-36 1715761-9 1991 Under conditions of TPA treatment in which protein kinase C was chronically reduced in T lymphocytes, IL-2 production was greatly depressed as were the level of IL-2 mRNA and [3H]thymidine incorporation. Tetradecanoylphorbol Acetate 20-23 interleukin 2 Homo sapiens 102-106 2026453-0 1991 High-affinity interleukin 2 receptors on B cell chronic lymphocytic leukemia cells are induced by phorbol myristate acetate but not by calcium ionophore. Tetradecanoylphorbol Acetate 98-123 interleukin 2 Homo sapiens 14-27 1715761-9 1991 Under conditions of TPA treatment in which protein kinase C was chronically reduced in T lymphocytes, IL-2 production was greatly depressed as were the level of IL-2 mRNA and [3H]thymidine incorporation. Tetradecanoylphorbol Acetate 20-23 interleukin 2 Homo sapiens 161-165 1903523-4 1991 High doses of recombinant IL-2, when added to in vitro cultures, were able to restore proliferation induced by phorbol myristate acetate and ionomycin but the response to concanavalin A remained severely defective. Tetradecanoylphorbol Acetate 111-136 interleukin 2 Homo sapiens 26-30 2026453-4 1991 Radiolabeled IL2 binding assays also demonstrated that PMA induced both high-affinity IL2R (HA-IL2R) and low-affinity IL2R (LA-IL2R) on B-CLL cells, but that A23187 did not. Tetradecanoylphorbol Acetate 55-58 interleukin 2 Homo sapiens 13-16 1847252-5 1991 PHA plus anti-CD28 or PMA plus anti-CD28-induced IL-2 synthesis was inhibited by genistein, and CsA, though it inhibited the PHA plus PMA-stimulated IL-2 synthesis, failed to have any effect on PMA plus anti-CD28-induced IL-2 synthesis. Tetradecanoylphorbol Acetate 22-25 interleukin 2 Homo sapiens 49-53 2146363-13 1990 In addition, PHA alone or in combination with PMA induced tumor necrosis factor alpha (TNF-alpha) and interferon gamma (IFN-gamma) (but not IL-2) production by CD3- thymocytes. Tetradecanoylphorbol Acetate 46-49 interleukin 2 Homo sapiens 140-144 1840028-8 1991 In addition, following stimulation with phytohemagglutinin (PHA) and phorbol 12-myristate 13-acetate (or PHA alone) all clones released interferon-gamma and tumour necrosis factor-alpha, but not IL-2. Tetradecanoylphorbol Acetate 69-100 interleukin 2 Homo sapiens 195-199 1988110-2 1991 The tumor-promoting phorbol ester 12-O-tetradecanoyl phorbol-13-acetate (TPA) can enhance this proliferation, partly because of an increase in interleukin 2 (IL-2) production. Tetradecanoylphorbol Acetate 34-71 interleukin 2 Homo sapiens 143-156 1988110-2 1991 The tumor-promoting phorbol ester 12-O-tetradecanoyl phorbol-13-acetate (TPA) can enhance this proliferation, partly because of an increase in interleukin 2 (IL-2) production. Tetradecanoylphorbol Acetate 34-71 interleukin 2 Homo sapiens 158-162 1988110-2 1991 The tumor-promoting phorbol ester 12-O-tetradecanoyl phorbol-13-acetate (TPA) can enhance this proliferation, partly because of an increase in interleukin 2 (IL-2) production. Tetradecanoylphorbol Acetate 73-76 interleukin 2 Homo sapiens 143-156 1988110-2 1991 The tumor-promoting phorbol ester 12-O-tetradecanoyl phorbol-13-acetate (TPA) can enhance this proliferation, partly because of an increase in interleukin 2 (IL-2) production. Tetradecanoylphorbol Acetate 73-76 interleukin 2 Homo sapiens 158-162 1988110-3 1991 However, if lymphocytes are treated with TPA for 24 h before concanavalin A exposure, IL-2 production and proliferation are depressed. Tetradecanoylphorbol Acetate 41-44 interleukin 2 Homo sapiens 86-90 1988110-6 1991 However, 12-deoxyphorbol 13-phenylacetate and 12-deoxyphorbol 13-phenylacetate-20-acetate were required at nearly 100-fold higher concentrations than TPA to suppress IL-2 production, suppress mitogenesis, and cause down-regulation of protein kinase C. A comparison of structures indicated that an R group at the 12-position was less important for IL-2 production and mitogenesis than for down-regulation of protein kinase C and the suppression of mitogenesis. Tetradecanoylphorbol Acetate 150-153 interleukin 2 Homo sapiens 166-170 1988110-6 1991 However, 12-deoxyphorbol 13-phenylacetate and 12-deoxyphorbol 13-phenylacetate-20-acetate were required at nearly 100-fold higher concentrations than TPA to suppress IL-2 production, suppress mitogenesis, and cause down-regulation of protein kinase C. A comparison of structures indicated that an R group at the 12-position was less important for IL-2 production and mitogenesis than for down-regulation of protein kinase C and the suppression of mitogenesis. Tetradecanoylphorbol Acetate 150-153 interleukin 2 Homo sapiens 347-351 2121373-1 1990 In this study, we investigated the biological effects of N-m-KRTLR using as an in vitro model the induction of the IL-2 receptor and IL-2 secretion by Jurkat cells in response to stimulation with 12-O tetradecanoylphorbol-13-acetate (TPA) plus phytohemagglutinin (PHA) and TPA plus OKT3 mAb. Tetradecanoylphorbol Acetate 196-232 interleukin 2 Homo sapiens 115-119 2121373-3 1990 Furthermore, N-m-KRTLR inhibited the production and release of IL-2 from cultured Jurkat cells stimulated with TPA plus either PHA or OKT3 mAb. Tetradecanoylphorbol Acetate 111-114 interleukin 2 Homo sapiens 63-67 2121373-4 1990 Similarly, this peptide significantly inhibited the IL-2 production in normal human peripheral blood mononuclear cells in response to stimulation by TPA and PHA. Tetradecanoylphorbol Acetate 149-152 interleukin 2 Homo sapiens 52-56 2204815-1 1990 The gene encoding interleukin-2 (IL-2) contains a sequence 52 to 326 nucleotides upstream of its transcriptional initiation site that promotes transcription in T cells that have been activated by costimulation with tetradecanoyl phorbol myristyl acetate (TPA) and phytohemagglutinin (PHA). Tetradecanoylphorbol Acetate 255-258 interleukin 2 Homo sapiens 18-31 2175060-10 1990 Only 53% of TIL clones released IL-2 in response to PHA + TPA stimulation, whereas 68% of PBL-derived clones were IL-2 producers. Tetradecanoylphorbol Acetate 58-61 interleukin 2 Homo sapiens 32-36 2204815-1 1990 The gene encoding interleukin-2 (IL-2) contains a sequence 52 to 326 nucleotides upstream of its transcriptional initiation site that promotes transcription in T cells that have been activated by costimulation with tetradecanoyl phorbol myristyl acetate (TPA) and phytohemagglutinin (PHA). Tetradecanoylphorbol Acetate 255-258 interleukin 2 Homo sapiens 33-37 2204815-3 1990 Each site in the IL-2 enhancer that bound Oct-1 in vitro was also required to achieve a maximal transcriptional response to TPA plus PHA in vivo. Tetradecanoylphorbol Acetate 124-127 interleukin 2 Homo sapiens 17-21 2159467-2 1990 Dexamethasone treatment of the Jurkat T77 cell clone inhibited the enhancing effect of 12-O-tetradecanoylporbol-13-acetate (TPA) and the calcium ionophore A23187 on the interleukin 2 (IL2) mRNA levels and gene transcription from intact nuclei. Tetradecanoylphorbol Acetate 124-127 interleukin 2 Homo sapiens 184-187 2143761-1 1990 Culture medium conditioned by phorbol 12-myristate 13-acetate-differentiated THP-1 cells contained interleukin 1 (IL-1) antagonist activity as measured by inhibition of both IL-1 beta binding to receptors on YT cells and inhibition of IL-1/phytohemagglutinin-stimulated IL-2 synthesis by LBRM-33-1A5 T cells. Tetradecanoylphorbol Acetate 30-61 interleukin 2 Homo sapiens 270-274 2118479-3 1990 Recently, AS101 and the protein kinase C (PKC) inducer, phorbol myristate acetate (PMA), were shown to synergize in the secretion of interleukin-2 (IL-2) and colony-stimulating factor (CSF) in vitro, by human and mouse lymphoid cells. Tetradecanoylphorbol Acetate 83-86 interleukin 2 Homo sapiens 148-152 2145219-9 1990 Addition of PMA induces both p70/75 and p50/55 IL2 receptor upregulation, as well as IL2-dependent proliferation. Tetradecanoylphorbol Acetate 12-15 interleukin 2 Homo sapiens 47-50 2145219-9 1990 Addition of PMA induces both p70/75 and p50/55 IL2 receptor upregulation, as well as IL2-dependent proliferation. Tetradecanoylphorbol Acetate 12-15 interleukin 2 Homo sapiens 85-88 2159467-3 1990 Dexamethasone treatment of Jurkat T77 cells inhibited the TPA/A23187-dependent activation of the transcription from the transfected pIL2CAT, containing 600 base pairs of the genomic sequences upstream of the coding region of IL2 gene, including the TPA/calcium responsive cis-regulatory elements and promoter sequences, driving the expression of the chloramphenicol acetyltransferase (CAT) gene. Tetradecanoylphorbol Acetate 58-61 interleukin 2 Homo sapiens 133-136 2212953-5 1990 Moreover, crosslinking native or truncated A2 or B27 induced IL-2 production upon co-stimulation with phorbol myristate acetate. Tetradecanoylphorbol Acetate 102-127 interleukin 2 Homo sapiens 61-65 2118479-3 1990 Recently, AS101 and the protein kinase C (PKC) inducer, phorbol myristate acetate (PMA), were shown to synergize in the secretion of interleukin-2 (IL-2) and colony-stimulating factor (CSF) in vitro, by human and mouse lymphoid cells. Tetradecanoylphorbol Acetate 56-81 interleukin 2 Homo sapiens 133-146 2118479-3 1990 Recently, AS101 and the protein kinase C (PKC) inducer, phorbol myristate acetate (PMA), were shown to synergize in the secretion of interleukin-2 (IL-2) and colony-stimulating factor (CSF) in vitro, by human and mouse lymphoid cells. Tetradecanoylphorbol Acetate 56-81 interleukin 2 Homo sapiens 148-152 2118479-3 1990 Recently, AS101 and the protein kinase C (PKC) inducer, phorbol myristate acetate (PMA), were shown to synergize in the secretion of interleukin-2 (IL-2) and colony-stimulating factor (CSF) in vitro, by human and mouse lymphoid cells. Tetradecanoylphorbol Acetate 83-86 interleukin 2 Homo sapiens 133-146 6808028-1 1982 In this study, we demonstrate that both highly purified T4+ and T8+ lymphocytes can produce substantial amounts of Interleukin 2(IL 2) when stimulated with the combination of concanavalin A (Con A) and phorbol myristate acetate. Tetradecanoylphorbol Acetate 202-227 interleukin 2 Homo sapiens 115-128 2158514-4 1990 In 8 of 10 patients studied, PMN capacity to oxidize intracellular dichlorofluorescein dye, an indirect measurement of O2- production in response to PMA stimulation, decreased after IL-2 administration (pre-IL-2 mean dichlorofluorescein oxidation (by channel number) 243 +/- 128 vs 3-day post-IL-2 87 +/- 86, p2 less than 0.02). Tetradecanoylphorbol Acetate 149-152 interleukin 2 Homo sapiens 182-186 1690769-6 1990 The association of the latter with PMA strongly induced the production of IL-2 on this cell model while either L161 or PMA alone had no effect. Tetradecanoylphorbol Acetate 35-38 interleukin 2 Homo sapiens 74-78 1974593-3 1990 The ability of the phorbol ester, phorbol 12-myristate 13-acetate (PMA), and the calcium ionophore, A23187, in co-stimulation with PHA, to enhance the IL-2 secretion, IL-2R expression and 3H thymidine incorporation were studied in the PBMC of colorectal cancer patients. Tetradecanoylphorbol Acetate 34-65 interleukin 2 Homo sapiens 151-155 1974593-3 1990 The ability of the phorbol ester, phorbol 12-myristate 13-acetate (PMA), and the calcium ionophore, A23187, in co-stimulation with PHA, to enhance the IL-2 secretion, IL-2R expression and 3H thymidine incorporation were studied in the PBMC of colorectal cancer patients. Tetradecanoylphorbol Acetate 67-70 interleukin 2 Homo sapiens 151-155 1974593-7 1990 A23187 is known to be effective in elevating cytosolic free Ca2+ and PMA is regarded as an activator of protein kinase C. Therefore, we may conclude that the impairment of IL-2 production and IL-2R expression in PHA-stimulated cultures is mainly due to failure of the free Ca2+ release which can be repaired by A23187. Tetradecanoylphorbol Acetate 69-72 interleukin 2 Homo sapiens 172-176 1973607-5 1990 PBLs of 18 BP patients showed an increase in IL2 production (1027.4 +/- 670.5 U/ml vs 270 +/- 100 U/ml in controls) during the acute stage of the disease after stimulation with phytohaemagglutinin (PHA)/phorbol myristate acetate (PMA). Tetradecanoylphorbol Acetate 203-228 interleukin 2 Homo sapiens 45-48 1973607-5 1990 PBLs of 18 BP patients showed an increase in IL2 production (1027.4 +/- 670.5 U/ml vs 270 +/- 100 U/ml in controls) during the acute stage of the disease after stimulation with phytohaemagglutinin (PHA)/phorbol myristate acetate (PMA). Tetradecanoylphorbol Acetate 230-233 interleukin 2 Homo sapiens 45-48 2107143-2 1990 In this study, we demonstrate that the IL-2 secretion and cell proliferation of both human and mouse lymphocytes, and the production of CSF by mouse spleen cells, was significantly enhanced by the synergistic effect of AS101 and phorbol myristate acetate (PMA). Tetradecanoylphorbol Acetate 229-254 interleukin 2 Homo sapiens 39-43 2107143-2 1990 In this study, we demonstrate that the IL-2 secretion and cell proliferation of both human and mouse lymphocytes, and the production of CSF by mouse spleen cells, was significantly enhanced by the synergistic effect of AS101 and phorbol myristate acetate (PMA). Tetradecanoylphorbol Acetate 256-259 interleukin 2 Homo sapiens 39-43 2150760-6 1990 In our experimental system, IL-2 production was observed either when L3T4-positive T cell hybridomas 2-45-12 were stimulated with ABA-L-Tyr and Ia molecules on the vesicles in the presence of phorbol 12-myristate 13-acetate, or when L3T4-negative T cell hybridomas 3H60.12 were incubated with ABA-L-Tyr and Ia molecules on the planar membranes. Tetradecanoylphorbol Acetate 192-223 interleukin 2 Homo sapiens 28-32 2139363-7 1990 Furthermore, when anti-CD3 and phorbol myristate acetate (PMA) were added together, they exerted a very marked synergistic effect on both the proliferation of, and IL 2 production by, cord PBMC. Tetradecanoylphorbol Acetate 31-56 interleukin 2 Homo sapiens 164-168 2139363-7 1990 Furthermore, when anti-CD3 and phorbol myristate acetate (PMA) were added together, they exerted a very marked synergistic effect on both the proliferation of, and IL 2 production by, cord PBMC. Tetradecanoylphorbol Acetate 58-61 interleukin 2 Homo sapiens 164-168 6808028-1 1982 In this study, we demonstrate that both highly purified T4+ and T8+ lymphocytes can produce substantial amounts of Interleukin 2(IL 2) when stimulated with the combination of concanavalin A (Con A) and phorbol myristate acetate. Tetradecanoylphorbol Acetate 202-227 interleukin 2 Homo sapiens 129-133 10097788-8 1999 RESULTS: Phorbol-myristate-acetate and ionomycine strongly increase the percent-age of IL-2+ cells; an additional 50% HCSs significantly suppresses the percentage to, or below the level of unstimulated cells. Tetradecanoylphorbol Acetate 9-34 interleukin 2 Homo sapiens 87-91 2483604-0 1989 Effects of phorbol myristate acetate on interleukin-2 and accompanying interferon production of human leukocytes induced by heat-inactivated Staphylococcus aureus. Tetradecanoylphorbol Acetate 11-36 interleukin 2 Homo sapiens 40-53 7880978-7 1994 IL-2 and IFN-gamma mRNA were detectable in PBMC as early as 3 hours after in vitro stimulation with PMA and ionomycin. Tetradecanoylphorbol Acetate 100-103 interleukin 2 Homo sapiens 0-4 34689117-6 2021 Furthermore, the level of cytokine production was measured in the Jurkat T cell line stimulated with phorbol 12-myristate 13-acetate/ionomycin and bavachin using an IL-2 ELISA and a cytometric bead array assay. Tetradecanoylphorbol Acetate 101-132 interleukin 2 Homo sapiens 165-169 34867961-9 2021 In stimulating peripheral blood mononuclear cells using PMA plus ionomycin, a high TIM3 level on T cells correlated with low interleukin-2 and tumor necrosis factor-alpha (TNF-alpha) on CD4+ cells and interferon-gamma and TNF-alpha on CD8+ T cells. Tetradecanoylphorbol Acetate 56-59 interleukin 2 Homo sapiens 125-138 34804038-7 2021 However, EBV exposure impaired the cytokine (IFN-gamma, IL-2, and TNF-alpha) secretion capability of CD4+ and CD8+ T cells after stimulation with PMA/ionomycin in vitro. Tetradecanoylphorbol Acetate 146-149 interleukin 2 Homo sapiens 56-60 35608955-4 2022 In contrast, following TCR-independent activation using PMA/ionomycin, neonatal cells demonstrated increased expression of CD69, IL-2 and TNF- alpha and equivalent phosphoERK compared to adult cells. Tetradecanoylphorbol Acetate 56-59 interleukin 2 Homo sapiens 129-133 2483604-1 1989 Interleukin-2 (IL-2) production induced by heat--inactivated Staphylococcus aureus (SAU) was enhanced by simultaneous addition of phorbol myristate acetate (PMA). Tetradecanoylphorbol Acetate 130-155 interleukin 2 Homo sapiens 0-13 2483604-1 1989 Interleukin-2 (IL-2) production induced by heat--inactivated Staphylococcus aureus (SAU) was enhanced by simultaneous addition of phorbol myristate acetate (PMA). Tetradecanoylphorbol Acetate 157-160 interleukin 2 Homo sapiens 0-13 2517125-3 1989 The depressed IL-2 production by SLE T cells are largely reversed by the addition of either phorbol ester (PMA) or partially by a calcium ionophore. Tetradecanoylphorbol Acetate 107-110 interleukin 2 Homo sapiens 14-18 2572284-4 1989 When the T cells were stimulated with phytohemagglutinin (PHA) and phorbol myristate acetate (PMA), defective interleukin-2 (IL-2) secretion was observed. Tetradecanoylphorbol Acetate 67-92 interleukin 2 Homo sapiens 110-123 2572284-4 1989 When the T cells were stimulated with phytohemagglutinin (PHA) and phorbol myristate acetate (PMA), defective interleukin-2 (IL-2) secretion was observed. Tetradecanoylphorbol Acetate 94-97 interleukin 2 Homo sapiens 110-123 2526179-4 1989 CD4+CD45R+ cells produced high levels of IL-2 mRNA when stimulated with either PMA together with calcium ionophore, or with PHA, but they expressed only trace quantities of mRNA for IL-4 or IFN-gamma. Tetradecanoylphorbol Acetate 79-82 interleukin 2 Homo sapiens 41-45 2594016-11 1989 However, CD4+ and CD8+ T cells produced large amounts of IL-2 when stimulated with mitogen and a protein kinase C activator, phorbol myristate acetate (PMA). Tetradecanoylphorbol Acetate 125-150 interleukin 2 Homo sapiens 57-61 2594016-11 1989 However, CD4+ and CD8+ T cells produced large amounts of IL-2 when stimulated with mitogen and a protein kinase C activator, phorbol myristate acetate (PMA). Tetradecanoylphorbol Acetate 152-155 interleukin 2 Homo sapiens 57-61 2510289-5 1989 However, the production of IL-2 was severely decreased in patient cells after stimulation with A23187/PMA (median 3541 units), although it was higher than in PHA-stimulated control cells (median 354 units). Tetradecanoylphorbol Acetate 102-105 interleukin 2 Homo sapiens 27-31 2745978-8 1989 TPA treatment of IL-2-dependent PBL at the peak of their growth caused phosphorylation of about two-thirds of preexisting unphosphorylated prosolin within 1 h. This was accompanied by cessation of cell proliferation, as indicated by measurements of TdR incorporation. Tetradecanoylphorbol Acetate 0-3 interleukin 2 Homo sapiens 17-21 2472451-3 1989 FK506 inhibited IL-2 mRNA accumulation in Con A, Con A plus PMA, Ionomycin plus PMA, anti-CD3, and anti-CD3 plus PMA activated T cells. Tetradecanoylphorbol Acetate 60-63 interleukin 2 Homo sapiens 16-20 2785816-1 1989 The kinetics of interleukin 2 mRNA accumulation in the leukemic T-cell line Jurkat, which can be induced with phytohemagglutinin and phorbol 12-myristate 13-acetate to produce large amounts of interleukin 2, was analyzed by a modified DNA-excess solution hybridization assay using a 5"-32P-labeled oligodeoxyribonucleotide 30 bases long as probe. Tetradecanoylphorbol Acetate 133-164 interleukin 2 Homo sapiens 16-29 2543699-5 1989 Stimulation of cells by optimal amounts of calcium ionophore and PMA induced IL-2 mRNA that was completely suppressed by cyclosporine. Tetradecanoylphorbol Acetate 65-68 interleukin 2 Homo sapiens 77-81 2543699-6 1989 The addition of anti-CD28 to T cells stimulated with PMA plus calcium ionophore induced a 5- to 100-fold increase in IL-2 gene expression and secretion that was resistant to cyclosporine. Tetradecanoylphorbol Acetate 53-56 interleukin 2 Homo sapiens 117-121 2543699-7 1989 The CD28 signal was able to increase steady state IL-2 mRNA levels even in cells treated with maximally tolerated amounts of calcium ionophore and PMA. Tetradecanoylphorbol Acetate 147-150 interleukin 2 Homo sapiens 50-54 2543699-9 1989 The signal provided by CD28 is distinct from that of CD3 because although anti-CD28 plus PMA-induced proliferation is resistant to cyclosporine, anti-CD3 or anti-CD3 plus PMA-induced IL-2 expression is sensitive. Tetradecanoylphorbol Acetate 89-92 interleukin 2 Homo sapiens 183-187 2785473-4 1989 Functionally, these T cell lines secreted IL-2 in response to stimulation with phytohemagglutinin (PHA) and phorbol 12-myristate 13-acetate (PMA) in combination, but not to PHA or PMA alone. Tetradecanoylphorbol Acetate 141-144 interleukin 2 Homo sapiens 42-46 2493338-5 1989 The production of IL-2 in normal lymphocytes stimulated with A23187/PMA was 33 times higher than that after stimulation with PHA. Tetradecanoylphorbol Acetate 68-71 interleukin 2 Homo sapiens 18-22 2493338-6 1989 In AIDS lymphocytes the production of IL-2 induced by all activators was severely decreased compared to control subjects, although the production of IL-2 after stimulation with A23187/PMA was higher than that in control lymphocytes after stimulation with PHA. Tetradecanoylphorbol Acetate 184-187 interleukin 2 Homo sapiens 38-42 2493338-6 1989 In AIDS lymphocytes the production of IL-2 induced by all activators was severely decreased compared to control subjects, although the production of IL-2 after stimulation with A23187/PMA was higher than that in control lymphocytes after stimulation with PHA. Tetradecanoylphorbol Acetate 184-187 interleukin 2 Homo sapiens 149-153 2521661-5 1989 The inhibitors which were effective on Ca2+ mobilization also inhibited IL-2 production initiated by an anti-CD3 mAb in the presence of 12-O-tetradecanoylphorbol-13-acetate, and to a lesser extent by PHA or the calcium ionophore A23187. Tetradecanoylphorbol Acetate 136-172 interleukin 2 Homo sapiens 72-76 2469725-6 1989 Cross-linking class I MHC molecules on Jurkat cells induced a rise by [Ca2+]i and induced IL-2 production upon co-stimulation with PMA. Tetradecanoylphorbol Acetate 131-134 interleukin 2 Homo sapiens 90-94 2544432-1 1989 Monoclonal antibodies (mAb) against CD3 or CD28 in conjunction with the tumor promoter phorbol 12-myristate 13-acetate (PMA) induce interleukin 2 receptor (IL2R) expression, IL2 production and proliferation in resting T cells. Tetradecanoylphorbol Acetate 87-118 interleukin 2 Homo sapiens 156-159 2544432-1 1989 Monoclonal antibodies (mAb) against CD3 or CD28 in conjunction with the tumor promoter phorbol 12-myristate 13-acetate (PMA) induce interleukin 2 receptor (IL2R) expression, IL2 production and proliferation in resting T cells. Tetradecanoylphorbol Acetate 120-123 interleukin 2 Homo sapiens 156-159 2785473-4 1989 Functionally, these T cell lines secreted IL-2 in response to stimulation with phytohemagglutinin (PHA) and phorbol 12-myristate 13-acetate (PMA) in combination, but not to PHA or PMA alone. Tetradecanoylphorbol Acetate 108-139 interleukin 2 Homo sapiens 42-46 2469910-4 1989 Treatment of the TsF2 producing hybridoma with phorbol myristate acetate (PMA) causes an increase in the level of IL-2 receptor expression in this hybridoma and enhances the effects of IL-2 on the biosynthesis of TsF2. Tetradecanoylphorbol Acetate 47-72 interleukin 2 Homo sapiens 114-118 2469910-4 1989 Treatment of the TsF2 producing hybridoma with phorbol myristate acetate (PMA) causes an increase in the level of IL-2 receptor expression in this hybridoma and enhances the effects of IL-2 on the biosynthesis of TsF2. Tetradecanoylphorbol Acetate 74-77 interleukin 2 Homo sapiens 114-118 2783885-10 1989 Addition of culture supernatants from phytohemagglutinin- and phorbol myristate acetate-stimulated peripheral blood mononuclear cells to the "Tac-negative" TIL-induced detectable Tac expression within 48 h. These results indicate that both non-Tac and Tac IL-2 receptors play important roles during IL-2-dependent proliferation of TIL. Tetradecanoylphorbol Acetate 62-87 interleukin 2 Homo sapiens 256-260 2783885-10 1989 Addition of culture supernatants from phytohemagglutinin- and phorbol myristate acetate-stimulated peripheral blood mononuclear cells to the "Tac-negative" TIL-induced detectable Tac expression within 48 h. These results indicate that both non-Tac and Tac IL-2 receptors play important roles during IL-2-dependent proliferation of TIL. Tetradecanoylphorbol Acetate 62-87 interleukin 2 Homo sapiens 299-303 2523864-1 1989 Recombinant IL-2 (rIL-2) and IL-4 (rIL-4) promote proliferation of human CD4+ T cells activated in the presence of PHA, TPA or OKT-3 monoclonal antibody (MAb), whereas the production of interferon-gamma (IFN) can be induced only by rIL-2. Tetradecanoylphorbol Acetate 120-123 interleukin 2 Homo sapiens 12-16 2642028-7 1989 In addition vanadate also induced IL-2 secretion in Jurkat cells when associated with the phorbol ester TPA, further demonstrating the importance of these phosphorylation reactions in the process of T cell activation. Tetradecanoylphorbol Acetate 104-107 interleukin 2 Homo sapiens 34-38 2481793-12 1989 Our data demonstrate that IL-2 and TNFs alpha and beta can act as growth factors for B-CLL cells under fully defined in vitro conditions, essentially without the need of TPA or anti-IgM as primary activation signals. Tetradecanoylphorbol Acetate 170-173 interleukin 2 Homo sapiens 26-30 15493263-1 1989 Phorbol myristate acetage (PMA) and Ca2+ ionophore A23187 mimic early signal transduction pathways and activate purified human T cells to secrete large quantities of interleukin-2 (IL-2) and to proliferate. Tetradecanoylphorbol Acetate 27-30 interleukin 2 Homo sapiens 166-179 15493263-1 1989 Phorbol myristate acetage (PMA) and Ca2+ ionophore A23187 mimic early signal transduction pathways and activate purified human T cells to secrete large quantities of interleukin-2 (IL-2) and to proliferate. Tetradecanoylphorbol Acetate 27-30 interleukin 2 Homo sapiens 181-185 3059076-8 1988 In contrast, B cells from 3 other patients responded well to IL-2 when preactivated for 24 h with phorbolester TPA and ionomycin. Tetradecanoylphorbol Acetate 111-114 interleukin 2 Homo sapiens 61-65 3143423-6 1988 The thapsigargin/PMA treatment caused an increase in the interleukin-2 (IL-2) production of the lymphocytes, which was much higher than that caused by the PHA treatment, even in AIDS lymphocytes. Tetradecanoylphorbol Acetate 17-20 interleukin 2 Homo sapiens 57-70 3143423-6 1988 The thapsigargin/PMA treatment caused an increase in the interleukin-2 (IL-2) production of the lymphocytes, which was much higher than that caused by the PHA treatment, even in AIDS lymphocytes. Tetradecanoylphorbol Acetate 17-20 interleukin 2 Homo sapiens 72-76 3143423-7 1988 Moreover, the thapsigargin/PMA treatment stimulated the expression of the IL-2 receptors on both normal and AIDS lymphocytes, similar to the effect of PHA. Tetradecanoylphorbol Acetate 27-30 interleukin 2 Homo sapiens 74-78 2902930-3 1988 The proliferative response was dependent upon an operational interleukin-2 (IL-2) system and exhibited a high degree of specificity; sn-1,2-diC8 was twice as active as racemic-1,2-diC8, and diC8 and TPA were not synergistic. Tetradecanoylphorbol Acetate 199-202 interleukin 2 Homo sapiens 76-80 2460372-1 1988 We previously established a human T cell line, TPA-Mat, which can proliferate in response to not only interleukin-2 (IL-2), but also phorbol esters such as 12-O-tetradecanoylphorbol-13-acetate (TPA) and phorbol-12,13-dibutyrate (PDBu). Tetradecanoylphorbol Acetate 47-50 interleukin 2 Homo sapiens 102-115 2460372-1 1988 We previously established a human T cell line, TPA-Mat, which can proliferate in response to not only interleukin-2 (IL-2), but also phorbol esters such as 12-O-tetradecanoylphorbol-13-acetate (TPA) and phorbol-12,13-dibutyrate (PDBu). Tetradecanoylphorbol Acetate 47-50 interleukin 2 Homo sapiens 117-121 2460372-2 1988 The present study demonstrated that the PDBu-dependent growth of TPA-Mat cells was inhibited up to 90% by adenosine 3",5"-cyclic monophosphate (cAMP] raising agents such as forskolin, cholera toxin and 1-methyl-3-isobutyl-xanthine, and cAMP analogues, whereas the IL-2-stimulated TPA-Mat growth was slightly inhibited. Tetradecanoylphorbol Acetate 65-68 interleukin 2 Homo sapiens 264-268 2460372-3 1988 These findings suggest that the signal transduction pathway of PDBu-induced growth, which should involve activation of protein kinase C, is sensitive to cAMP, and that it cannot be exactly identical to the signal transduction pathway of Il-2-induced growth in TPA-Mat cells. Tetradecanoylphorbol Acetate 260-263 interleukin 2 Homo sapiens 237-241 2970356-6 1988 Endogenous production of IL-2 after stimulation with PHA and phorbol myristate acetate was positive in 3/9 CD3- and in 8/8 CD3+, CD4-, CD8- clones. Tetradecanoylphorbol Acetate 61-86 interleukin 2 Homo sapiens 25-29 3264806-0 1988 Phorbol myristate acetate induces IL-2 secretion by HUT 78 cells by a mechanism independent of protein kinase C translocation. Tetradecanoylphorbol Acetate 0-25 interleukin 2 Homo sapiens 34-38 3261728-1 1988 A selected clone from an IL-2-dependent human T-cell line was persistently propagated in the presence of phorbol esters with the ability to activate protein kinase C (PKC), such as 12-O-tetradecanoylphorbol-13-acetate (TPA) or phorbol-12,13-dibutylate (PDBu). Tetradecanoylphorbol Acetate 181-217 interleukin 2 Homo sapiens 25-29 2971552-4 1988 In order to observe levels of interleukin 2 mRNA, it was necessary to use phorbol myristate acetate (PMA) in addition to either lectins or anti-CD3 antibodies. Tetradecanoylphorbol Acetate 74-99 interleukin 2 Homo sapiens 30-43 2971552-4 1988 In order to observe levels of interleukin 2 mRNA, it was necessary to use phorbol myristate acetate (PMA) in addition to either lectins or anti-CD3 antibodies. Tetradecanoylphorbol Acetate 101-104 interleukin 2 Homo sapiens 30-43 3261728-8 1988 These observations suggest that the sustained activation of PKC by the phorbol esters could induce continuous growth of the IL-2-dependent TPA-Mat cells. Tetradecanoylphorbol Acetate 139-142 interleukin 2 Homo sapiens 124-128 3261728-2 1988 Thus, a TPA(PDBu)-dependent T-cell line, designated TPA-Mat, was established from IL-2-dependent T cells. Tetradecanoylphorbol Acetate 8-11 interleukin 2 Homo sapiens 82-86 3261728-2 1988 Thus, a TPA(PDBu)-dependent T-cell line, designated TPA-Mat, was established from IL-2-dependent T cells. Tetradecanoylphorbol Acetate 52-55 interleukin 2 Homo sapiens 82-86 3261728-5 1988 Therefore, the phorbol esters substituted for IL-2 and may be directly involved in transduction of growth signals in TPA-Mat cells. Tetradecanoylphorbol Acetate 117-120 interleukin 2 Homo sapiens 46-50 3137654-0 1988 Modulation of high-affinity interleukin 2 receptors on activated human T lymphocytes by activators of protein kinase C. Phorbol myristate acetate (PMA) and 1-oleoyl-2-acetyl-rac-glycerol (OAG) are shown to induce a rapid (within 30 min) down-regulation of the capacity of activated human T lymphocytes to bind interleukin 2. Tetradecanoylphorbol Acetate 120-145 interleukin 2 Homo sapiens 28-41 3137654-0 1988 Modulation of high-affinity interleukin 2 receptors on activated human T lymphocytes by activators of protein kinase C. Phorbol myristate acetate (PMA) and 1-oleoyl-2-acetyl-rac-glycerol (OAG) are shown to induce a rapid (within 30 min) down-regulation of the capacity of activated human T lymphocytes to bind interleukin 2. Tetradecanoylphorbol Acetate 147-150 interleukin 2 Homo sapiens 28-41 3122759-1 1988 Human peripheral blood lymphocytes secrete high titers of interleukin-2 (IL-2) after stimulation by Ca2+-ionophore A23187/phorbol 12-myristate-13-acetate. Tetradecanoylphorbol Acetate 122-153 interleukin 2 Homo sapiens 58-71 3260781-2 1988 Deficiency in PHA-stimulated IL-2 production by cells from SLE patients was repaired by the addition of PMA, but not ionomycin. Tetradecanoylphorbol Acetate 104-107 interleukin 2 Homo sapiens 29-33 3260781-3 1988 PMA alone did not stimulate IL-2 production but, in concert with PHA, induced IL-2 synthesis. Tetradecanoylphorbol Acetate 0-3 interleukin 2 Homo sapiens 78-82 3260781-4 1988 Moreover, PMA was effective in the repair of the deficiency of PHA-induced IL-2 production by both T4+ and T8+ subsets. Tetradecanoylphorbol Acetate 10-13 interleukin 2 Homo sapiens 75-79 3258883-2 1988 Substantial levels of IL-2 responsiveness were induced in T8+ cells by lectin, Con A, mAb directed against the CD3 Ag, OKT3, Ca2+ ionophore, ionomycin or phorbol ester, PMA. Tetradecanoylphorbol Acetate 169-172 interleukin 2 Homo sapiens 22-26 2835170-8 1988 Finally, progression to the proliferative phase of LGL, activated by TPA alone or with ionomycin, was completely abrogated by a hyperimmune anti-IL-2 antiserum. Tetradecanoylphorbol Acetate 69-72 interleukin 2 Homo sapiens 145-149 2451522-1 1988 Previous work from our laboratory demonstrated the mitogenic response of human peripheral blood T lymphocytes by phytohemagglutinin (PHA), phorbol myristate acetate (PMA) and ionomycin, or interleukin 2 (IL-2). Tetradecanoylphorbol Acetate 166-169 interleukin 2 Homo sapiens 204-208 3127526-4 1988 When lymphocyte preparations were costimulated with PMA, the TSST-1 effect was strongly potentiated and the levels of cytotoxic factors, IFN-gamma, and IL-2 present in supernatant fluids were comparable to those observed after treatment with PMA and PHA. Tetradecanoylphorbol Acetate 52-55 interleukin 2 Homo sapiens 152-156 3260781-0 1988 Deficient phytohemagglutinin-induced interleukin-2 activity in patients with inactive systemic lupus erythematosus is correctable by the addition of phorbol myristate acetate. Tetradecanoylphorbol Acetate 149-174 interleukin 2 Homo sapiens 37-50 3260781-1 1988 In systemic lupus erythematosus (SLE) patients, the production of interleukin-2 (IL-2) by blood T lymphocytes in response to stimulation with phytohemagglutinin (PHA) either alone or with phorbol myristate acetate (PMA) or ionomycin, a Ca2+ ionophore, was examined. Tetradecanoylphorbol Acetate 188-213 interleukin 2 Homo sapiens 66-79 3260781-1 1988 In systemic lupus erythematosus (SLE) patients, the production of interleukin-2 (IL-2) by blood T lymphocytes in response to stimulation with phytohemagglutinin (PHA) either alone or with phorbol myristate acetate (PMA) or ionomycin, a Ca2+ ionophore, was examined. Tetradecanoylphorbol Acetate 188-213 interleukin 2 Homo sapiens 81-85 3260781-1 1988 In systemic lupus erythematosus (SLE) patients, the production of interleukin-2 (IL-2) by blood T lymphocytes in response to stimulation with phytohemagglutinin (PHA) either alone or with phorbol myristate acetate (PMA) or ionomycin, a Ca2+ ionophore, was examined. Tetradecanoylphorbol Acetate 215-218 interleukin 2 Homo sapiens 66-79 3260781-1 1988 In systemic lupus erythematosus (SLE) patients, the production of interleukin-2 (IL-2) by blood T lymphocytes in response to stimulation with phytohemagglutinin (PHA) either alone or with phorbol myristate acetate (PMA) or ionomycin, a Ca2+ ionophore, was examined. Tetradecanoylphorbol Acetate 215-218 interleukin 2 Homo sapiens 81-85 3138125-7 1988 Peripheral blood T-lymphocyte IL 2 production at onset of IDDM was normal under basal conditions, and upon optimal stimulation with concanavalin A (ConA) and phorbol myristate acetate (PMA). Tetradecanoylphorbol Acetate 158-183 interleukin 2 Homo sapiens 30-34 3138125-7 1988 Peripheral blood T-lymphocyte IL 2 production at onset of IDDM was normal under basal conditions, and upon optimal stimulation with concanavalin A (ConA) and phorbol myristate acetate (PMA). Tetradecanoylphorbol Acetate 185-188 interleukin 2 Homo sapiens 30-34 3131868-4 1988 If AC or 12-O-tetradecanoyl-phorbol-13-acetate (TPA) were also present, IL-2 production and DNA synthesis were seen in both subsets. Tetradecanoylphorbol Acetate 9-46 interleukin 2 Homo sapiens 72-76 3123493-4 1988 Phorbol 12-myristate 13-acetate (PMA) induced IL 2 receptor expression on II23 cells but not IL 2 secretion. Tetradecanoylphorbol Acetate 0-31 interleukin 2 Homo sapiens 46-50 3123493-4 1988 Phorbol 12-myristate 13-acetate (PMA) induced IL 2 receptor expression on II23 cells but not IL 2 secretion. Tetradecanoylphorbol Acetate 33-36 interleukin 2 Homo sapiens 46-50 3126303-4 1988 The combination of A23187 and TPA significantly (p less than 0.005-0.001) enhanced IL2 secretion in patients" cell cultures (range, 20 to greater than 64 U/ml). Tetradecanoylphorbol Acetate 30-33 interleukin 2 Homo sapiens 83-86 3122759-1 1988 Human peripheral blood lymphocytes secrete high titers of interleukin-2 (IL-2) after stimulation by Ca2+-ionophore A23187/phorbol 12-myristate-13-acetate. Tetradecanoylphorbol Acetate 122-153 interleukin 2 Homo sapiens 73-77 3261032-0 1988 Phorbol myristate acetate (PMA) reverses inhibition of interleukin-2 production by T lymphocytes of patients with systemic lupus erythematosus. Tetradecanoylphorbol Acetate 0-25 interleukin 2 Homo sapiens 55-68 2826588-6 1988 Fluoride ions were found to be as effective as tetradecanoyl phorbol acetate to stimulate IL-2 synthesis in Jurkat cells when used in combination with phytohemagglutinin. Tetradecanoylphorbol Acetate 47-76 interleukin 2 Homo sapiens 90-94 3257464-4 1988 IL-2 suppressed the stimulatory effects of both the chemotactic peptide formyl-methionyl-leucyl-phenyl-alanine (FMLP) and the phorbol ester phorbol myristate acetate (PMA). Tetradecanoylphorbol Acetate 140-165 interleukin 2 Homo sapiens 0-4 3257464-4 1988 IL-2 suppressed the stimulatory effects of both the chemotactic peptide formyl-methionyl-leucyl-phenyl-alanine (FMLP) and the phorbol ester phorbol myristate acetate (PMA). Tetradecanoylphorbol Acetate 167-170 interleukin 2 Homo sapiens 0-4 3259277-12 1988 Moreover, when the cells were pre-activated with SAC or with PHA plus TPA and then further stimulated with IL-2, a 2-20 fold increase in proliferative response was found in the majority of cases studied. Tetradecanoylphorbol Acetate 70-73 interleukin 2 Homo sapiens 107-111 3261032-0 1988 Phorbol myristate acetate (PMA) reverses inhibition of interleukin-2 production by T lymphocytes of patients with systemic lupus erythematosus. Tetradecanoylphorbol Acetate 27-30 interleukin 2 Homo sapiens 55-68 3261032-3 1988 The depressed IL-2 production by SLE T cells was largely reversed by the addition of phorbol myristate acetate (PMA), which directly activates protein kinase C. This can explain why some authors using PMA together with mitogens were not able to find a depressed IL-2 production in SLE patients. Tetradecanoylphorbol Acetate 85-110 interleukin 2 Homo sapiens 14-18 3261032-3 1988 The depressed IL-2 production by SLE T cells was largely reversed by the addition of phorbol myristate acetate (PMA), which directly activates protein kinase C. This can explain why some authors using PMA together with mitogens were not able to find a depressed IL-2 production in SLE patients. Tetradecanoylphorbol Acetate 85-110 interleukin 2 Homo sapiens 262-266 3261032-3 1988 The depressed IL-2 production by SLE T cells was largely reversed by the addition of phorbol myristate acetate (PMA), which directly activates protein kinase C. This can explain why some authors using PMA together with mitogens were not able to find a depressed IL-2 production in SLE patients. Tetradecanoylphorbol Acetate 112-115 interleukin 2 Homo sapiens 14-18 3261032-3 1988 The depressed IL-2 production by SLE T cells was largely reversed by the addition of phorbol myristate acetate (PMA), which directly activates protein kinase C. This can explain why some authors using PMA together with mitogens were not able to find a depressed IL-2 production in SLE patients. Tetradecanoylphorbol Acetate 112-115 interleukin 2 Homo sapiens 262-266 2822168-8 1987 The low affinity binding of 125I-IL-2 to TPA-stimulated leukemia cells was observed in the three cases of pre-T-ALL tested, and the addition of recombinant IL-2 to TPA-stimulated cells showed no effect on cell proliferation. Tetradecanoylphorbol Acetate 41-44 interleukin 2 Homo sapiens 33-37 3123107-3 1987 Using phorbol myristate acetate (PMA), which directly activates PKC, and Ca2+ ionophore A23187, which increases intracellular cytoplasmic free Ca2+ concentration, the induction of IL-2 secretion, IL-2R expression and cell proliferation were studied. Tetradecanoylphorbol Acetate 6-31 interleukin 2 Homo sapiens 180-184 3123107-3 1987 Using phorbol myristate acetate (PMA), which directly activates PKC, and Ca2+ ionophore A23187, which increases intracellular cytoplasmic free Ca2+ concentration, the induction of IL-2 secretion, IL-2R expression and cell proliferation were studied. Tetradecanoylphorbol Acetate 33-36 interleukin 2 Homo sapiens 180-184 3123107-4 1987 The results demonstrate that following stimulation with PMA and A23187, purified T cells from elderly subjects demonstrate low levels of IL-2 production, IL-2R expression and cell proliferation. Tetradecanoylphorbol Acetate 56-59 interleukin 2 Homo sapiens 137-141 2822168-8 1987 The low affinity binding of 125I-IL-2 to TPA-stimulated leukemia cells was observed in the three cases of pre-T-ALL tested, and the addition of recombinant IL-2 to TPA-stimulated cells showed no effect on cell proliferation. Tetradecanoylphorbol Acetate 164-167 interleukin 2 Homo sapiens 33-37 2822168-8 1987 The low affinity binding of 125I-IL-2 to TPA-stimulated leukemia cells was observed in the three cases of pre-T-ALL tested, and the addition of recombinant IL-2 to TPA-stimulated cells showed no effect on cell proliferation. Tetradecanoylphorbol Acetate 164-167 interleukin 2 Homo sapiens 156-160 3116098-0 1987 Large scale production and purification of human IL-2 from buffy coat lymphocytes stimulated with 12-O-tetradecanoylphorbol 13-acetate and calcium ionophore A23187. Tetradecanoylphorbol Acetate 98-134 interleukin 2 Homo sapiens 49-53 3116098-4 1987 This degree of lymphocyte purity was important since phagocytes were inhibitory to 12-O-tetradecanoylphorbol 13-acetate/calcium ionophore (TPA/A23187)-induced IL-2 production when their concentration exceeded 15% of the total cells. Tetradecanoylphorbol Acetate 83-119 interleukin 2 Homo sapiens 159-163 3116098-4 1987 This degree of lymphocyte purity was important since phagocytes were inhibitory to 12-O-tetradecanoylphorbol 13-acetate/calcium ionophore (TPA/A23187)-induced IL-2 production when their concentration exceeded 15% of the total cells. Tetradecanoylphorbol Acetate 139-142 interleukin 2 Homo sapiens 159-163 3116098-6 1987 Using TPA/A23187 induction, up to 500 micrograms of IL-2 per liter were produced. Tetradecanoylphorbol Acetate 6-9 interleukin 2 Homo sapiens 52-56 3115657-4 1987 At low concentrations (0.1 microM), A23187 synergized maximally with PMA to induce proliferation, to increase IL-2R mRNA levels and the expression of membrane IL-2R, and to produce a low but sufficient accumulation of IL-2 mRNA and IL-2 secretion. Tetradecanoylphorbol Acetate 69-72 interleukin 2 Homo sapiens 110-114 2960536-10 1987 Phorbol myristate acetate but not mAb 72-5D3 induced proliferation of MHDC when recombinant interleukin 2 (rIL2) was added. Tetradecanoylphorbol Acetate 0-25 interleukin 2 Homo sapiens 92-105 3118114-6 1987 Cross-linking of 125I-labeled IL-2 to TPA activated B cell precursor ALL revealed the 55 kD Tac/CD25 protein and an additional protein of 75 kD. Tetradecanoylphorbol Acetate 38-41 interleukin 2 Homo sapiens 30-34 3496927-10 1987 B cells activated with either anti-Ig or TPA proliferated in the presence of IL-2, whereas B-CLL cells did not, although they all expressed the identical 60-kilodalton proteins by immunoprecipitation. Tetradecanoylphorbol Acetate 41-44 interleukin 2 Homo sapiens 77-81 3115657-4 1987 At low concentrations (0.1 microM), A23187 synergized maximally with PMA to induce proliferation, to increase IL-2R mRNA levels and the expression of membrane IL-2R, and to produce a low but sufficient accumulation of IL-2 mRNA and IL-2 secretion. Tetradecanoylphorbol Acetate 69-72 interleukin 2 Homo sapiens 159-163 3116404-3 1987 Lymphokine mRNA was induced by stimulating the cells with the phorbol diester PMA (TPA), with or without T-lymphocyte mitogens. Tetradecanoylphorbol Acetate 83-86 interleukin 2 Homo sapiens 0-10 2885371-3 1987 To further study the functional role of this molecule, Thy-1-negative variants were selected and analyzed for IL 2 production in response to phorbol-12-myristate-13-acetate (PMA) or to Con A. Tetradecanoylphorbol Acetate 141-172 interleukin 2 Homo sapiens 110-114 2885371-3 1987 To further study the functional role of this molecule, Thy-1-negative variants were selected and analyzed for IL 2 production in response to phorbol-12-myristate-13-acetate (PMA) or to Con A. Tetradecanoylphorbol Acetate 174-177 interleukin 2 Homo sapiens 110-114 2887717-6 1987 Although the cell preparations were relatively independent of exogenous IL-2 for the proliferative response to the combined stimulation of ionomycin and PMA at usual culture cell density, they needed exogenous IL-2 for sustained proliferation at lower culture cell density (5 X 10(3)/ml). Tetradecanoylphorbol Acetate 153-156 interleukin 2 Homo sapiens 72-76 2957786-4 1987 However, DNA synthesis was observed when recombinant interleukin 2 (IL-2) or other secondary signals, such as those provided by phorbol myristate acetate (PMA) or autologous accessory cells (AC), were also added. Tetradecanoylphorbol Acetate 155-158 interleukin 2 Homo sapiens 53-66 3106472-8 1987 We confirmed that a combination of TPA and lectins or TPA and anti-CD3 mAb is necessary to obtain full activation of Jurkat cells if this event is monitored by using measurement of IL 2 synthesis. Tetradecanoylphorbol Acetate 35-38 interleukin 2 Homo sapiens 181-185 3106472-8 1987 We confirmed that a combination of TPA and lectins or TPA and anti-CD3 mAb is necessary to obtain full activation of Jurkat cells if this event is monitored by using measurement of IL 2 synthesis. Tetradecanoylphorbol Acetate 54-57 interleukin 2 Homo sapiens 181-185 3106472-10 1987 Indeed, a combination of TPA and one of these two drugs induced maximal IL 2 synthesis. Tetradecanoylphorbol Acetate 25-28 interleukin 2 Homo sapiens 72-76 3496450-3 1987 Indomethacin, phorbol myristate acetate and irradiation of suppressor cells increased IL-2 values in rheumatoid SF and peripheral blood but did not restore normal IL-2 production. Tetradecanoylphorbol Acetate 14-39 interleukin 2 Homo sapiens 86-90 3495445-6 1987 Activation markers such as the transferrin and interleukin 2 (IL2) receptors were markedly increased after 24 h incubation with TPA and ionomycin. Tetradecanoylphorbol Acetate 128-131 interleukin 2 Homo sapiens 47-60 3495445-6 1987 Activation markers such as the transferrin and interleukin 2 (IL2) receptors were markedly increased after 24 h incubation with TPA and ionomycin. Tetradecanoylphorbol Acetate 128-131 interleukin 2 Homo sapiens 62-65 3104454-1 1987 Concanavalin A (Con A), which together with phorbol myristate acetate (PMA) can activate the human T cell line Jurkat to produce interleukin 2 (IL 2), is shown to depend on the expression of the T3/T cell antigen receptor heterodimer (T3/Ti) complex to induce activation. Tetradecanoylphorbol Acetate 44-69 interleukin 2 Homo sapiens 129-142 3104454-1 1987 Concanavalin A (Con A), which together with phorbol myristate acetate (PMA) can activate the human T cell line Jurkat to produce interleukin 2 (IL 2), is shown to depend on the expression of the T3/T cell antigen receptor heterodimer (T3/Ti) complex to induce activation. Tetradecanoylphorbol Acetate 44-69 interleukin 2 Homo sapiens 144-148 3104454-1 1987 Concanavalin A (Con A), which together with phorbol myristate acetate (PMA) can activate the human T cell line Jurkat to produce interleukin 2 (IL 2), is shown to depend on the expression of the T3/T cell antigen receptor heterodimer (T3/Ti) complex to induce activation. Tetradecanoylphorbol Acetate 71-74 interleukin 2 Homo sapiens 129-142 3104454-1 1987 Concanavalin A (Con A), which together with phorbol myristate acetate (PMA) can activate the human T cell line Jurkat to produce interleukin 2 (IL 2), is shown to depend on the expression of the T3/T cell antigen receptor heterodimer (T3/Ti) complex to induce activation. Tetradecanoylphorbol Acetate 71-74 interleukin 2 Homo sapiens 144-148 2953065-4 1987 The removal of PMA and subsequent addition of recombinant interleukin 2 (IL-2) or IL-2-containing supernatants effectively reversed the effect of PMA with recovery of antigen-specific lytic function of cells treated with 10(-8) M, while cells treated with 10(-5)M PMA only recovered lectin-dependent cytotoxic ability. Tetradecanoylphorbol Acetate 146-149 interleukin 2 Homo sapiens 58-71 2953065-4 1987 The removal of PMA and subsequent addition of recombinant interleukin 2 (IL-2) or IL-2-containing supernatants effectively reversed the effect of PMA with recovery of antigen-specific lytic function of cells treated with 10(-8) M, while cells treated with 10(-5)M PMA only recovered lectin-dependent cytotoxic ability. Tetradecanoylphorbol Acetate 146-149 interleukin 2 Homo sapiens 73-77 2953065-4 1987 The removal of PMA and subsequent addition of recombinant interleukin 2 (IL-2) or IL-2-containing supernatants effectively reversed the effect of PMA with recovery of antigen-specific lytic function of cells treated with 10(-8) M, while cells treated with 10(-5)M PMA only recovered lectin-dependent cytotoxic ability. Tetradecanoylphorbol Acetate 146-149 interleukin 2 Homo sapiens 82-86 2953065-4 1987 The removal of PMA and subsequent addition of recombinant interleukin 2 (IL-2) or IL-2-containing supernatants effectively reversed the effect of PMA with recovery of antigen-specific lytic function of cells treated with 10(-8) M, while cells treated with 10(-5)M PMA only recovered lectin-dependent cytotoxic ability. Tetradecanoylphorbol Acetate 146-149 interleukin 2 Homo sapiens 58-71 2953065-4 1987 The removal of PMA and subsequent addition of recombinant interleukin 2 (IL-2) or IL-2-containing supernatants effectively reversed the effect of PMA with recovery of antigen-specific lytic function of cells treated with 10(-8) M, while cells treated with 10(-5)M PMA only recovered lectin-dependent cytotoxic ability. Tetradecanoylphorbol Acetate 146-149 interleukin 2 Homo sapiens 73-77 2953065-4 1987 The removal of PMA and subsequent addition of recombinant interleukin 2 (IL-2) or IL-2-containing supernatants effectively reversed the effect of PMA with recovery of antigen-specific lytic function of cells treated with 10(-8) M, while cells treated with 10(-5)M PMA only recovered lectin-dependent cytotoxic ability. Tetradecanoylphorbol Acetate 146-149 interleukin 2 Homo sapiens 82-86 2951735-9 1987 Analysis of the functional responsiveness of the three distinct groups of JA3 cell variants to different stimuli showed that all produced interleukin 2 in response to A23187 calcium ionophore plus phorbol 12-myristate 13-acetate. Tetradecanoylphorbol Acetate 197-228 interleukin 2 Homo sapiens 138-151 3097142-0 1987 Interleukin 2 responses of lpr and normal L3T4-/Lyt-2- T cells induced by TPA plus A23187. Tetradecanoylphorbol Acetate 74-77 interleukin 2 Homo sapiens 0-13 3102668-10 1987 In contrast, calcium ionophore plus PMA did induce the expression of a linked gene through the IL-2 5" flanking region in the mutant Jurkat cell line. Tetradecanoylphorbol Acetate 36-39 interleukin 2 Homo sapiens 95-99 2437015-4 1987 Treatment of T cells with TPA appears to bypass the requirement for an increase in [Ca2+]i for IL2 secretion and cell proliferation, indicating that various mitogens can trigger T cells through both [Ca2+]i-dependent and [Ca2+]i-independent pathways. Tetradecanoylphorbol Acetate 26-29 interleukin 2 Homo sapiens 95-98 3501185-4 1987 Dexamethasone and Cyclosporin A (CsA) which inhibit early events of T cell activation and the expression of the interleukin-2 (IL-2) and gamma-interferon (gamma-IFN) genes also markedly suppress the expression of c-myc mRNA in Con A, and Con A + TPA-activated thymocytes. Tetradecanoylphorbol Acetate 246-249 interleukin 2 Homo sapiens 112-125 3501185-4 1987 Dexamethasone and Cyclosporin A (CsA) which inhibit early events of T cell activation and the expression of the interleukin-2 (IL-2) and gamma-interferon (gamma-IFN) genes also markedly suppress the expression of c-myc mRNA in Con A, and Con A + TPA-activated thymocytes. Tetradecanoylphorbol Acetate 246-249 interleukin 2 Homo sapiens 127-131 3093070-5 1986 1,25-(OH)2D3 inhibited the expression of interleukin 2 and gamma-interferon messenger RNA in human lymphocytes activated by phytohemagglutinin and 12-O-tetradecanoylphorbol-13-acetate. Tetradecanoylphorbol Acetate 147-183 interleukin 2 Homo sapiens 41-54 3026658-2 1986 We used the cholera toxin (CT), a cAMP elevating agent, to study the influence of this nucleotide on the production of interleukin 2 (IL-2) by human peripheral blood mononuclear cells stimulated by phytohemagglutinin and phorbol myristate acetate. Tetradecanoylphorbol Acetate 221-246 interleukin 2 Homo sapiens 119-132 3026658-2 1986 We used the cholera toxin (CT), a cAMP elevating agent, to study the influence of this nucleotide on the production of interleukin 2 (IL-2) by human peripheral blood mononuclear cells stimulated by phytohemagglutinin and phorbol myristate acetate. Tetradecanoylphorbol Acetate 221-246 interleukin 2 Homo sapiens 134-138 3490990-8 1986 In cultures stimulated with either TPA or PHA, approximately equal numbers of colonies are generated in the presence of IL-2, suggesting that T4 and T8 cells have similar proliferative capabilities. Tetradecanoylphorbol Acetate 35-38 interleukin 2 Homo sapiens 120-124 3102134-2 1986 The present study shows that relatively immature T6+ human thymocytes as well as the more mature T3+ thymocytes could be induced to express functional IL-2 receptors when activated with either Concanavalin A (Con A), Con A and 12-O-tetradecanoylphorbol 13-acetate (TPA) or IL-2 in combination with Con A or TPA. Tetradecanoylphorbol Acetate 227-263 interleukin 2 Homo sapiens 151-155 3490288-14 1986 IL-2 and TPA, however, had profound influence on the NK function of the cells with enhancement in the case of IL-2 and marked suppression when stimulated by TPA. Tetradecanoylphorbol Acetate 9-12 interleukin 2 Homo sapiens 110-114 3490288-14 1986 IL-2 and TPA, however, had profound influence on the NK function of the cells with enhancement in the case of IL-2 and marked suppression when stimulated by TPA. Tetradecanoylphorbol Acetate 157-160 interleukin 2 Homo sapiens 0-4 3094603-2 1986 The proliferative response was correlated with the inducibility of receptors for IL 2 on the surface membrane of T-ALL and T-NHL cells by incubation with TPA or PHA for 18 hours. Tetradecanoylphorbol Acetate 154-157 interleukin 2 Homo sapiens 81-85 3094603-4 1986 Accordingly, receptors for IL 2, initially absent from the cell surface, were found on high proportions of the T-ALL and T-NHL cells after in vitro exposure to TPA. Tetradecanoylphorbol Acetate 160-163 interleukin 2 Homo sapiens 27-31 3094603-10 1986 The results from this study indicate that the requirements of endogenous v exogenous IL 2 for cell proliferation in T-ALL and T-NHL and IL 2 receptor activation by PHA and TPA vary from patient to patient. Tetradecanoylphorbol Acetate 172-175 interleukin 2 Homo sapiens 136-140 3102134-2 1986 The present study shows that relatively immature T6+ human thymocytes as well as the more mature T3+ thymocytes could be induced to express functional IL-2 receptors when activated with either Concanavalin A (Con A), Con A and 12-O-tetradecanoylphorbol 13-acetate (TPA) or IL-2 in combination with Con A or TPA. Tetradecanoylphorbol Acetate 265-268 interleukin 2 Homo sapiens 151-155 3102134-2 1986 The present study shows that relatively immature T6+ human thymocytes as well as the more mature T3+ thymocytes could be induced to express functional IL-2 receptors when activated with either Concanavalin A (Con A), Con A and 12-O-tetradecanoylphorbol 13-acetate (TPA) or IL-2 in combination with Con A or TPA. Tetradecanoylphorbol Acetate 307-310 interleukin 2 Homo sapiens 151-155 2878123-8 1986 The phorbol ester, phorbol 12-myristate 13-acetate (PMA) in combination with phytohemagglutinin or concanavalin A, were required for maximal release of LT and IL-2. Tetradecanoylphorbol Acetate 19-50 interleukin 2 Homo sapiens 159-163 3093578-4 1986 When stimulated in short-term culture with a combination of phorbol ester (PMA) and calcium ionophore, Lyt-2-/L3T4- thymocytes proliferated in a largely IL 2-dependent fashion. Tetradecanoylphorbol Acetate 75-78 interleukin 2 Homo sapiens 153-157 2878123-8 1986 The phorbol ester, phorbol 12-myristate 13-acetate (PMA) in combination with phytohemagglutinin or concanavalin A, were required for maximal release of LT and IL-2. Tetradecanoylphorbol Acetate 52-55 interleukin 2 Homo sapiens 159-163 2878123-10 1986 Soluble anti-CD3 was not sufficient to induce IL-2 or LT release from the II-23 hybrid; however, soluble anti-CD3 combined with PMA was a strong stimulus for lymphotoxin and IL-2 release. Tetradecanoylphorbol Acetate 128-131 interleukin 2 Homo sapiens 174-178