PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 31217526-10 2020 In vitro experiments also showed that neutrophils expressed a GnRH receptor and that GnRH agonist treatment increased NETosis markers and promoted phorbol myristate acetate (PMA)-induced NETosis in mouse and human neutrophils. Tetradecanoylphorbol Acetate 147-172 gonadotropin releasing hormone 1 Mus musculus 85-89 31217526-10 2020 In vitro experiments also showed that neutrophils expressed a GnRH receptor and that GnRH agonist treatment increased NETosis markers and promoted phorbol myristate acetate (PMA)-induced NETosis in mouse and human neutrophils. Tetradecanoylphorbol Acetate 174-177 gonadotropin releasing hormone 1 Mus musculus 85-89 18931473-3 2009 In the present study, focusing mainly on PKC epsilon, the mechanisms underlying the proteasome-dependent downregulation of GnRH-activated PKC epsilon and TPA-sensitive PKC alpha and epsilon isoenzymes were investigated in alphaT3-1 gonadotrope cells. Tetradecanoylphorbol Acetate 154-157 gonadotropin releasing hormone 1 Mus musculus 123-127 15994198-4 2005 Several of these effectors act through membrane receptors to trigger the protein kinase C pathway, and 12-O-tetradecanoyl phorbol-13-acetate (TPA), a modulator of this pathway, has been shown to suppress GnRH gene expression through the promoter. Tetradecanoylphorbol Acetate 103-140 gonadotropin releasing hormone 1 Mus musculus 204-208 16741954-7 2006 All of these effects of GnRH were mimicked by phorbol 12 myristate 13-acetate (PMA). Tetradecanoylphorbol Acetate 46-77 gonadotropin releasing hormone 1 Mus musculus 24-28 16741954-7 2006 All of these effects of GnRH were mimicked by phorbol 12 myristate 13-acetate (PMA). Tetradecanoylphorbol Acetate 79-82 gonadotropin releasing hormone 1 Mus musculus 24-28 17446183-5 2007 Activators of cAMP and protein kinase C like forskolin and phorbol 12-myristate 3-acetate (PMA), respectively, mimicked GnRH action. Tetradecanoylphorbol Acetate 91-94 gonadotropin releasing hormone 1 Mus musculus 120-124 16888195-4 2006 Despite its positive coupling to cAMP pathway, GnRH counteracted PACAP induction of cAMP and this effect was mimicked by the PKC activator phorbol 12-myristate 13-acetate (PMA). Tetradecanoylphorbol Acetate 139-170 gonadotropin releasing hormone 1 Mus musculus 47-51 16888195-4 2006 Despite its positive coupling to cAMP pathway, GnRH counteracted PACAP induction of cAMP and this effect was mimicked by the PKC activator phorbol 12-myristate 13-acetate (PMA). Tetradecanoylphorbol Acetate 172-175 gonadotropin releasing hormone 1 Mus musculus 47-51 15994198-4 2005 Several of these effectors act through membrane receptors to trigger the protein kinase C pathway, and 12-O-tetradecanoyl phorbol-13-acetate (TPA), a modulator of this pathway, has been shown to suppress GnRH gene expression through the promoter. Tetradecanoylphorbol Acetate 142-145 gonadotropin releasing hormone 1 Mus musculus 204-208 15994198-5 2005 We find that TPA suppresses expression through the GnRH enhancer as well as the promoter. Tetradecanoylphorbol Acetate 13-16 gonadotropin releasing hormone 1 Mus musculus 51-55 15994198-9 2005 It is known that cooperative interaction among multiple factors is necessary for GnRH gene expression; thus, one mechanism by which TPA suppresses GnRH gene expression is to disengage some of these factors from their cis-regulatory elements. Tetradecanoylphorbol Acetate 132-135 gonadotropin releasing hormone 1 Mus musculus 81-85 15994198-9 2005 It is known that cooperative interaction among multiple factors is necessary for GnRH gene expression; thus, one mechanism by which TPA suppresses GnRH gene expression is to disengage some of these factors from their cis-regulatory elements. Tetradecanoylphorbol Acetate 132-135 gonadotropin releasing hormone 1 Mus musculus 147-151 9689135-7 1998 Finally, we established that photonic activity accurately reflected endogenous GnRH gene expression by treating transfected GT1-1 cells with phorbol 12-myristate 13 acetate (a consensus inhibitor of GnRH gene expression) and observing a dramatic suppression of photonic emissions from continuously monitored cells. Tetradecanoylphorbol Acetate 141-172 gonadotropin releasing hormone 1 Mus musculus 79-83 9891005-6 1999 Furthermore, repression of GnRH gene transcription by 12-O-tetradecanoylphorbol-13-acetate, which utilizes sequences that overlap with the nGRE, is reversed by this distal nGRE mutation leading to activation of GnRH gene transcription. Tetradecanoylphorbol Acetate 54-90 gonadotropin releasing hormone 1 Mus musculus 27-31 9891005-6 1999 Furthermore, repression of GnRH gene transcription by 12-O-tetradecanoylphorbol-13-acetate, which utilizes sequences that overlap with the nGRE, is reversed by this distal nGRE mutation leading to activation of GnRH gene transcription. Tetradecanoylphorbol Acetate 54-90 gonadotropin releasing hormone 1 Mus musculus 211-215 9689135-7 1998 Finally, we established that photonic activity accurately reflected endogenous GnRH gene expression by treating transfected GT1-1 cells with phorbol 12-myristate 13 acetate (a consensus inhibitor of GnRH gene expression) and observing a dramatic suppression of photonic emissions from continuously monitored cells. Tetradecanoylphorbol Acetate 141-172 gonadotropin releasing hormone 1 Mus musculus 199-203 9783907-9 1998 AlphaT3-1 cells possess several PKC isoforms which, except PKCzeta, were differentially down-regulated by TPA (PKCalpha, betaII, delta, epsilon, eta) or GnRH (PKCbetaII, delta, epsilon, eta). Tetradecanoylphorbol Acetate 106-109 gonadotropin releasing hormone 1 Mus musculus 111-190 9783907-11 1998 In conclusion, in alphaT3-1 cells the GnRH-induced homologous desensitization affects the GnRH coupling with PLC, PLA2 and PLD by mechanism(s) which do not implicate TPA-sensitive PKC isoforms, but likely reflect time-dependent modification(s) on the activation processes of the enzymes. Tetradecanoylphorbol Acetate 166-169 gonadotropin releasing hormone 1 Mus musculus 38-42 9783907-5 1998 GnRH-induced IPs desensitization was potentiated after direct activation of PKC by the phorbol ester TPA, suggesting the involvement of distinct mechanisms in the uncoupling exerted by either GnRH or TPA on GnRH-stimulated PI hydrolysis. Tetradecanoylphorbol Acetate 101-104 gonadotropin releasing hormone 1 Mus musculus 0-4 9783907-5 1998 GnRH-induced IPs desensitization was potentiated after direct activation of PKC by the phorbol ester TPA, suggesting the involvement of distinct mechanisms in the uncoupling exerted by either GnRH or TPA on GnRH-stimulated PI hydrolysis. Tetradecanoylphorbol Acetate 200-203 gonadotropin releasing hormone 1 Mus musculus 0-4 9427323-1 1997 Activation of PKC with 12-tetra-decanoylphorbol-13-acetate (TPA) or inhibition with staurosporine or calphostin C down-regulated GnRH mRNA levels. Tetradecanoylphorbol Acetate 60-63 gonadotropin releasing hormone 1 Mus musculus 129-133 9783907-8 1998 Under GnRH desensitization conditions, TPA was still able to induce PLD activation and to further potentiate the GnRH-evoked PLD activity. Tetradecanoylphorbol Acetate 39-42 gonadotropin releasing hormone 1 Mus musculus 6-10 9783907-8 1998 Under GnRH desensitization conditions, TPA was still able to induce PLD activation and to further potentiate the GnRH-evoked PLD activity. Tetradecanoylphorbol Acetate 39-42 gonadotropin releasing hormone 1 Mus musculus 113-117 9121495-1 1996 Phorbol ester 12-O-tetradecanoyl phorbol-13-acetate (TPA) regulation of the GnRH gene was studied in two mouse GnRH neuronal cell lines, Gn11 and NLT. Tetradecanoylphorbol Acetate 14-51 gonadotropin releasing hormone 1 Mus musculus 76-80 9147290-1 1997 GT1-7 cells respond to treatment with the phorbol ester, phorbol 12-myristate 13-acetate (PMA), with an inhibition of transcription of the proGnRH gene and decreases in GnRH mRNA levels. Tetradecanoylphorbol Acetate 57-88 gonadotropin releasing hormone 1 Mus musculus 142-146 9147290-1 1997 GT1-7 cells respond to treatment with the phorbol ester, phorbol 12-myristate 13-acetate (PMA), with an inhibition of transcription of the proGnRH gene and decreases in GnRH mRNA levels. Tetradecanoylphorbol Acetate 90-93 gonadotropin releasing hormone 1 Mus musculus 142-146 9121495-1 1996 Phorbol ester 12-O-tetradecanoyl phorbol-13-acetate (TPA) regulation of the GnRH gene was studied in two mouse GnRH neuronal cell lines, Gn11 and NLT. Tetradecanoylphorbol Acetate 53-56 gonadotropin releasing hormone 1 Mus musculus 76-80 9121495-1 1996 Phorbol ester 12-O-tetradecanoyl phorbol-13-acetate (TPA) regulation of the GnRH gene was studied in two mouse GnRH neuronal cell lines, Gn11 and NLT. Tetradecanoylphorbol Acetate 53-56 gonadotropin releasing hormone 1 Mus musculus 111-115 9121495-4 1996 In contrast, TPA treatment stimulated expression of a human GnRH-luciferase reporter construct, correlating with the expression of the protoon-cogenes c-fos and c-jun. Tetradecanoylphorbol Acetate 13-16 gonadotropin releasing hormone 1 Mus musculus 60-64 8243334-3 1993 GnRH action was mimicked by phorbol 12-myristate-13-acetate (PMA), but stimulation of Ca2+ entry by K(+)-induced depolarization and Bay K 8644 was much less effective. Tetradecanoylphorbol Acetate 28-59 gonadotropin releasing hormone 1 Mus musculus 0-4 8536629-7 1996 Pretreatment with TPA, however, decreased both GnRH- and TPA-induced MAPK activation, suggesting that PKC is involved in GnRH-mediated activation of MAPK. Tetradecanoylphorbol Acetate 18-21 gonadotropin releasing hormone 1 Mus musculus 47-51 8536629-7 1996 Pretreatment with TPA, however, decreased both GnRH- and TPA-induced MAPK activation, suggesting that PKC is involved in GnRH-mediated activation of MAPK. Tetradecanoylphorbol Acetate 18-21 gonadotropin releasing hormone 1 Mus musculus 121-125 8536629-7 1996 Pretreatment with TPA, however, decreased both GnRH- and TPA-induced MAPK activation, suggesting that PKC is involved in GnRH-mediated activation of MAPK. Tetradecanoylphorbol Acetate 57-60 gonadotropin releasing hormone 1 Mus musculus 121-125 8206325-1 1994 We previously showed that activation of protein kinase C (PKC) with the phorbol ester, 12-O-tetradecanoylphorbol 13-acetate (TPA) in GT1-7 hypothalamic cells decreases GnRH mRNA levels in a dose and time dependent fashion. Tetradecanoylphorbol Acetate 87-123 gonadotropin releasing hormone 1 Mus musculus 168-172 8206325-1 1994 We previously showed that activation of protein kinase C (PKC) with the phorbol ester, 12-O-tetradecanoylphorbol 13-acetate (TPA) in GT1-7 hypothalamic cells decreases GnRH mRNA levels in a dose and time dependent fashion. Tetradecanoylphorbol Acetate 125-128 gonadotropin releasing hormone 1 Mus musculus 168-172 7523206-3 1994 Prolonged treatment with forskolin (10 microM), ionomycin (1 microM) and PMA (10 nM) for 12 or 24 h resulted in significant decreases in GnRH mRNA levels. Tetradecanoylphorbol Acetate 73-76 gonadotropin releasing hormone 1 Mus musculus 137-141 7523206-4 1994 Time-course studies showed that the increases in GnRH secretion stimulated by forskolin, ionomycin and PMA were gradually attenuated over time in parallel with the decreases in mRNA expression. Tetradecanoylphorbol Acetate 103-106 gonadotropin releasing hormone 1 Mus musculus 49-53 8243334-3 1993 GnRH action was mimicked by phorbol 12-myristate-13-acetate (PMA), but stimulation of Ca2+ entry by K(+)-induced depolarization and Bay K 8644 was much less effective. Tetradecanoylphorbol Acetate 61-64 gonadotropin releasing hormone 1 Mus musculus 0-4 1446598-2 1992 Dose-response and time-course studies revealed that TPA (10(-8) M) acutely increased GnRH secretion 3-fold at 3-6 h, which then declined to baseline at 24 h, while it progressively decreased GnRH mRNA levels by 50% and 70% at 6 and 24 h, respectively. Tetradecanoylphorbol Acetate 52-55 gonadotropin releasing hormone 1 Mus musculus 85-89 8504741-6 1993 Thus, since extended exposure to TPA can down-regulate PKC, suppression of GnRH mRNA by TPA may be due to decreased PKC activity, indicating a role for PKC in the maintenance of the GnRH gene expression (a role that is unlikely to involve c-fos). Tetradecanoylphorbol Acetate 33-36 gonadotropin releasing hormone 1 Mus musculus 182-186 8504741-6 1993 Thus, since extended exposure to TPA can down-regulate PKC, suppression of GnRH mRNA by TPA may be due to decreased PKC activity, indicating a role for PKC in the maintenance of the GnRH gene expression (a role that is unlikely to involve c-fos). Tetradecanoylphorbol Acetate 88-91 gonadotropin releasing hormone 1 Mus musculus 75-79 8504741-6 1993 Thus, since extended exposure to TPA can down-regulate PKC, suppression of GnRH mRNA by TPA may be due to decreased PKC activity, indicating a role for PKC in the maintenance of the GnRH gene expression (a role that is unlikely to involve c-fos). Tetradecanoylphorbol Acetate 88-91 gonadotropin releasing hormone 1 Mus musculus 182-186 8504741-7 1993 In transient transfections, the transcriptional activity from 3 kilobases of GnRH 5"-flanking sequence was repressed 2-fold by either 100 nM TPA or 20 microM NPC 15437 at 24 h, demonstrating that suppression of GnRH mRNA is at least, in part, at the level of transcription. Tetradecanoylphorbol Acetate 141-144 gonadotropin releasing hormone 1 Mus musculus 77-81 8504741-7 1993 In transient transfections, the transcriptional activity from 3 kilobases of GnRH 5"-flanking sequence was repressed 2-fold by either 100 nM TPA or 20 microM NPC 15437 at 24 h, demonstrating that suppression of GnRH mRNA is at least, in part, at the level of transcription. Tetradecanoylphorbol Acetate 141-144 gonadotropin releasing hormone 1 Mus musculus 211-215 8504741-9 1993 Enhancement of GnRH secretion by TPA was robust and rapid (2.5 min), while the response to forskolin was relatively delayed (2 h). Tetradecanoylphorbol Acetate 33-36 gonadotropin releasing hormone 1 Mus musculus 15-19 1446598-2 1992 Dose-response and time-course studies revealed that TPA (10(-8) M) acutely increased GnRH secretion 3-fold at 3-6 h, which then declined to baseline at 24 h, while it progressively decreased GnRH mRNA levels by 50% and 70% at 6 and 24 h, respectively. Tetradecanoylphorbol Acetate 52-55 gonadotropin releasing hormone 1 Mus musculus 191-195 1446598-4 1992 This brief exposure to TPA also resulted in a decrease (60%) in GnRH mRNA levels at 6 h, with a 1.5- to 2-fold increase in GnRH secretion compared to control values, suggesting that activation of PKC decreases the pretranslational expression of GnRH while increasing GnRH secretion. Tetradecanoylphorbol Acetate 23-26 gonadotropin releasing hormone 1 Mus musculus 64-68 1446598-4 1992 This brief exposure to TPA also resulted in a decrease (60%) in GnRH mRNA levels at 6 h, with a 1.5- to 2-fold increase in GnRH secretion compared to control values, suggesting that activation of PKC decreases the pretranslational expression of GnRH while increasing GnRH secretion. Tetradecanoylphorbol Acetate 23-26 gonadotropin releasing hormone 1 Mus musculus 123-127 1446598-4 1992 This brief exposure to TPA also resulted in a decrease (60%) in GnRH mRNA levels at 6 h, with a 1.5- to 2-fold increase in GnRH secretion compared to control values, suggesting that activation of PKC decreases the pretranslational expression of GnRH while increasing GnRH secretion. Tetradecanoylphorbol Acetate 23-26 gonadotropin releasing hormone 1 Mus musculus 123-127 1446598-4 1992 This brief exposure to TPA also resulted in a decrease (60%) in GnRH mRNA levels at 6 h, with a 1.5- to 2-fold increase in GnRH secretion compared to control values, suggesting that activation of PKC decreases the pretranslational expression of GnRH while increasing GnRH secretion. Tetradecanoylphorbol Acetate 23-26 gonadotropin releasing hormone 1 Mus musculus 123-127 1446598-7 1992 We conclude that TPA, by activating the PKC pathway, acutely increases GnRH secretion, but dramatically decreases GnRH gene expression. Tetradecanoylphorbol Acetate 17-20 gonadotropin releasing hormone 1 Mus musculus 71-75 1446598-7 1992 We conclude that TPA, by activating the PKC pathway, acutely increases GnRH secretion, but dramatically decreases GnRH gene expression. Tetradecanoylphorbol Acetate 17-20 gonadotropin releasing hormone 1 Mus musculus 114-118 1511786-6 1992 The PKC agonist PMA (phorbol 12-myristate 13-acetate, 10(-6) M) produced a small rise in basal [Ca2+]i and abolished the GnRH calcium response. Tetradecanoylphorbol Acetate 16-19 gonadotropin releasing hormone 1 Mus musculus 121-125 1511786-6 1992 The PKC agonist PMA (phorbol 12-myristate 13-acetate, 10(-6) M) produced a small rise in basal [Ca2+]i and abolished the GnRH calcium response. Tetradecanoylphorbol Acetate 21-52 gonadotropin releasing hormone 1 Mus musculus 121-125