PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 27586664-7 2016 We describe dye uptake, interpreted as connexin dependent, that is shown to be enhanced with reduced extracellular Ca2+, mechanically responsive, inhibited by TPA, inhibited by EL186 antibodies for Cx43 and sustained for more than 15 min following mechanical stimulation. Tetradecanoylphorbol Acetate 159-162 gap junction protein alpha 1 Homo sapiens 198-202 19258009-3 2009 The tumor-promoting phorbol ester TPA strongly inhibits Cx43 gap junction channels. Tetradecanoylphorbol Acetate 34-37 gap junction protein alpha 1 Homo sapiens 56-60 24654232-8 2014 Stimulation of control SSM with phorbol 12-myristate 13-acetate (PMA), a PKC activator, increased both calcium tolerance and mitochondrial Cx43 phosphorylation at S262 and S368. Tetradecanoylphorbol Acetate 32-63 gap junction protein alpha 1 Homo sapiens 139-143 24654232-8 2014 Stimulation of control SSM with phorbol 12-myristate 13-acetate (PMA), a PKC activator, increased both calcium tolerance and mitochondrial Cx43 phosphorylation at S262 and S368. Tetradecanoylphorbol Acetate 65-68 gap junction protein alpha 1 Homo sapiens 139-143 22623726-5 2012 Here, we demonstrate by live-cell imaging analysis that treatment of cells with 12-O-tetradecanoylphorbol 13-acetate (TPA) induces endocytosis of subdomains of connexin43 gap junctions. Tetradecanoylphorbol Acetate 80-116 gap junction protein alpha 1 Homo sapiens 160-170 22623726-5 2012 Here, we demonstrate by live-cell imaging analysis that treatment of cells with 12-O-tetradecanoylphorbol 13-acetate (TPA) induces endocytosis of subdomains of connexin43 gap junctions. Tetradecanoylphorbol Acetate 118-121 gap junction protein alpha 1 Homo sapiens 160-170 22623726-7 2012 The HECT E3 ubiquitin ligase smad ubiquitination regulatory factor-2 (Smurf2) was found to be recruited to connexin43 gap junctions in response to TPA treatment. Tetradecanoylphorbol Acetate 147-150 gap junction protein alpha 1 Homo sapiens 107-117 20406988-5 2010 Interestingly, the association of Cx43 with Cav-1 was found to be reduced after TPA and EGF treatment. Tetradecanoylphorbol Acetate 80-83 gap junction protein alpha 1 Homo sapiens 34-38 22623726-9 2012 Smurf2 depletion also counteracted the TPA-induced endocytosis and degradation of connexin43. Tetradecanoylphorbol Acetate 39-42 gap junction protein alpha 1 Homo sapiens 82-92 21042803-8 2011 Western blot analyses revealed that EC and EGCG prevented down-regulation of Cx26 and Cx43 proteins in HaCaT cells treated with PMA. Tetradecanoylphorbol Acetate 128-131 gap junction protein alpha 1 Homo sapiens 86-90 21042803-9 2011 Immunocytochemistry showed decreased expression and abnormal location of Cx26 and Cx43 in HaCaT cells when treated with PMA, and EC and EGCG inhibited its effect. Tetradecanoylphorbol Acetate 120-123 gap junction protein alpha 1 Homo sapiens 82-86 20687122-8 2011 The gap junction connexin43 likely involved in the communication between the two cell types, because 12-O-tetradecanoylphorbol 13-acetate (TPA) could partially block cellular crosstalk. Tetradecanoylphorbol Acetate 101-137 gap junction protein alpha 1 Homo sapiens 17-27 20687122-8 2011 The gap junction connexin43 likely involved in the communication between the two cell types, because 12-O-tetradecanoylphorbol 13-acetate (TPA) could partially block cellular crosstalk. Tetradecanoylphorbol Acetate 139-142 gap junction protein alpha 1 Homo sapiens 17-27 21028875-2 2010 TPA inhibited GJIC in a dose-dependent and reversible manner and was associated with connexin 43 phosphorylation. Tetradecanoylphorbol Acetate 0-3 gap junction protein alpha 1 Homo sapiens 85-96 21028875-3 2010 Pretreatment of 20 muM c9,t11-CLA for 24 h prior to 60 nM TPA for 1 h prevented the inhibition of GJIC by reducing the phosphorylation of connexin 43 via suppressing extracellular signal-regulated kinases (ERK1/2) activation. Tetradecanoylphorbol Acetate 58-61 gap junction protein alpha 1 Homo sapiens 138-149 19258009-4 2009 In this study we have investigated mechanisms involved in TPA-induced phosphorylation of Cx43 and inhibition of gap junction channels. Tetradecanoylphorbol Acetate 58-61 gap junction protein alpha 1 Homo sapiens 89-93 19258009-6 2009 The data suggest that PKC-induced activation of MAP kinase partly involves Src-independent trans-activation of the EGF receptor, and that TPA-induced shift in SDS-PAGE gel mobility of Cx43 is caused by MAP kinase phosphorylation, whereas phosphorylation of S368 by PKC does not alter gel migration of Cx43. Tetradecanoylphorbol Acetate 138-141 gap junction protein alpha 1 Homo sapiens 184-188 19258009-6 2009 The data suggest that PKC-induced activation of MAP kinase partly involves Src-independent trans-activation of the EGF receptor, and that TPA-induced shift in SDS-PAGE gel mobility of Cx43 is caused by MAP kinase phosphorylation, whereas phosphorylation of S368 by PKC does not alter gel migration of Cx43. Tetradecanoylphorbol Acetate 138-141 gap junction protein alpha 1 Homo sapiens 301-305 18635599-7 2008 We show that lipid rafts are required for enterocyte migration, that IFN displaces Cx43 from lipid rafts, and that the phorbol ester phorbol 12-myristate 13-acetate (PMA) restores Cx43 to lipid rafts after treatment with IFN in a protein kinase C-dependent manner. Tetradecanoylphorbol Acetate 133-164 gap junction protein alpha 1 Homo sapiens 180-184 19134409-6 2008 In PMA and PKCalpha-asODN treated group, the level of p-PKCalpha decreased, the expression of ERK1/2, p-ERK1/2 and the expression of cyclinD1, P21(cip1) decreased correspondingly (the A value % control was 1.23 +/- 0.19, 1.34 +/- 0.18, 1.52 +/- 0.20, 1.45 +/- 0.18 and 1.49 +/- 0.18 respectively; q value was 7.49, 3.58, 5.97, 6.06 and 15.65 respectively; all P < 0.05), and cells proliferation reduced significantly [the percentage of cells in S phase was (21.2 +/- 2.8)%, A(490) value was 0.51 +/- 0.04; q = 6.07, 12.63; all P < 0.05], as compared with those of the PMA treated group. Tetradecanoylphorbol Acetate 3-6 gap junction protein alpha 1 Homo sapiens 54-64 19134409-7 2008 In PMA and U0126 treated group, the level of p-PKCalpha had no significant change (A value was1.99 +/- 0.18, q = 0.94, P > 0.05), but the levels of ERK1/2, p-ERK1/2 decreased, the expression of cyclinD1, P21(cip1) reduced (the A value % control was 0.95 +/- 0.21, 1.15 +/- 0.19, 1.37 +/- 0.15 and 1.96 +/- 0.21 respectively; q value was 7.79, 9.16, 6.92 and 11.16 respectively; all P < 0.05), and cells proliferation reduced significantly [the percentage of cells in S phase was (22.0 +/- 3.2)%, A(490) value was 0.49 +/- 0.03; q = 5.51, 13.45; all P < 0.05], as compared with those of the PMA treated group. Tetradecanoylphorbol Acetate 3-6 gap junction protein alpha 1 Homo sapiens 45-55 18635599-7 2008 We show that lipid rafts are required for enterocyte migration, that IFN displaces Cx43 from lipid rafts, and that the phorbol ester phorbol 12-myristate 13-acetate (PMA) restores Cx43 to lipid rafts after treatment with IFN in a protein kinase C-dependent manner. Tetradecanoylphorbol Acetate 166-169 gap junction protein alpha 1 Homo sapiens 180-184 17210245-1 2007 We have previously reported that protein kinase C gamma (PKC-gamma) is activated by phorbol-12-myristate-13-acetate (TPA) and that this causes PKC-gamma translocation to membranes and phosphorylation of the gap junction protein, connexin 43 (Cx43). Tetradecanoylphorbol Acetate 84-115 gap junction protein alpha 1 Homo sapiens 229-240 18172602-3 2008 In this work, we used the patch-clamp technique to study the effect of phorbol 12-myristate 13-acetate (PMA), a general PKC activator, on the electrical conductance of exogenous Cx43Hc expressed in tsA201 cells. Tetradecanoylphorbol Acetate 71-102 gap junction protein alpha 1 Homo sapiens 178-182 18172602-3 2008 In this work, we used the patch-clamp technique to study the effect of phorbol 12-myristate 13-acetate (PMA), a general PKC activator, on the electrical conductance of exogenous Cx43Hc expressed in tsA201 cells. Tetradecanoylphorbol Acetate 104-107 gap junction protein alpha 1 Homo sapiens 178-182 17210245-1 2007 We have previously reported that protein kinase C gamma (PKC-gamma) is activated by phorbol-12-myristate-13-acetate (TPA) and that this causes PKC-gamma translocation to membranes and phosphorylation of the gap junction protein, connexin 43 (Cx43). Tetradecanoylphorbol Acetate 84-115 gap junction protein alpha 1 Homo sapiens 242-246 17210245-1 2007 We have previously reported that protein kinase C gamma (PKC-gamma) is activated by phorbol-12-myristate-13-acetate (TPA) and that this causes PKC-gamma translocation to membranes and phosphorylation of the gap junction protein, connexin 43 (Cx43). Tetradecanoylphorbol Acetate 117-120 gap junction protein alpha 1 Homo sapiens 229-240 17210245-1 2007 We have previously reported that protein kinase C gamma (PKC-gamma) is activated by phorbol-12-myristate-13-acetate (TPA) and that this causes PKC-gamma translocation to membranes and phosphorylation of the gap junction protein, connexin 43 (Cx43). Tetradecanoylphorbol Acetate 117-120 gap junction protein alpha 1 Homo sapiens 242-246 15811935-9 2005 Treatment of cells with the protein kinase C activator 12-O-tetradecanoylphorbol-13-acetate increased the phosphorylated species of Cx43 and correspondingly inhibited GJIC. Tetradecanoylphorbol Acetate 55-91 gap junction protein alpha 1 Homo sapiens 132-136 12567182-11 2003 Intercellular communication is inhibited by TPA and can be restored by proteasome inhibitors, probably by preventing loss of Cx43 from the plasma membrane following treatment with TPA. Tetradecanoylphorbol Acetate 44-47 gap junction protein alpha 1 Homo sapiens 125-129 14563393-11 2003 These changes in the expression of Cx43 induced by OBC were similar to those induced by 12-O-tetradecanoylphorbol-13-acetate (TPA), a tumor promoter. Tetradecanoylphorbol Acetate 126-129 gap junction protein alpha 1 Homo sapiens 35-39 12697837-1 2003 Phorbol esters such as 12-O-tetradeconylphorbol-13-acetate (TPA) activate protein kinase C, increase Connexin43 (Cx43) phosphorylation, and decrease cell-cell communication via gap junctions in many cell types. Tetradecanoylphorbol Acetate 60-63 gap junction protein alpha 1 Homo sapiens 101-111 12697837-1 2003 Phorbol esters such as 12-O-tetradeconylphorbol-13-acetate (TPA) activate protein kinase C, increase Connexin43 (Cx43) phosphorylation, and decrease cell-cell communication via gap junctions in many cell types. Tetradecanoylphorbol Acetate 60-63 gap junction protein alpha 1 Homo sapiens 113-117 12697837-4 2003 We show that this antibody detects Cx43 only when it is phosphorylated at S368 and, consistent with previous results, TPA treatment causes a dramatic increase in phosphorylation at S368. Tetradecanoylphorbol Acetate 118-121 gap junction protein alpha 1 Homo sapiens 35-39 12567182-11 2003 Intercellular communication is inhibited by TPA and can be restored by proteasome inhibitors, probably by preventing loss of Cx43 from the plasma membrane following treatment with TPA. Tetradecanoylphorbol Acetate 180-183 gap junction protein alpha 1 Homo sapiens 125-129 12039800-10 2002 Treatment with the protein kinase C activator 12-O-tetradecanoylphorbol 13-acetate reduced junctional conductance in cells expressing Cx43, Cx45, or both connexins, but it reduced the extent of neurobiotin transfer only in HeLa-Cx43(His)(6) and HeLa-Cx43(His)(6)/Cx45 cells but not in the HeLa-Cx45 cells. Tetradecanoylphorbol Acetate 46-82 gap junction protein alpha 1 Homo sapiens 134-138 14694601-3 2002 RESULTS: Western-blot analyses of SiO2- and phorbol 12-myristate 13-acetate(TPA)-treated CCL-64 cells showed the same phosphorylation states of Cx43 as the control group. Tetradecanoylphorbol Acetate 44-75 gap junction protein alpha 1 Homo sapiens 144-148 14694601-3 2002 RESULTS: Western-blot analyses of SiO2- and phorbol 12-myristate 13-acetate(TPA)-treated CCL-64 cells showed the same phosphorylation states of Cx43 as the control group. Tetradecanoylphorbol Acetate 76-79 gap junction protein alpha 1 Homo sapiens 144-148 12039800-10 2002 Treatment with the protein kinase C activator 12-O-tetradecanoylphorbol 13-acetate reduced junctional conductance in cells expressing Cx43, Cx45, or both connexins, but it reduced the extent of neurobiotin transfer only in HeLa-Cx43(His)(6) and HeLa-Cx43(His)(6)/Cx45 cells but not in the HeLa-Cx45 cells. Tetradecanoylphorbol Acetate 46-82 gap junction protein alpha 1 Homo sapiens 228-232 12039800-10 2002 Treatment with the protein kinase C activator 12-O-tetradecanoylphorbol 13-acetate reduced junctional conductance in cells expressing Cx43, Cx45, or both connexins, but it reduced the extent of neurobiotin transfer only in HeLa-Cx43(His)(6) and HeLa-Cx43(His)(6)/Cx45 cells but not in the HeLa-Cx45 cells. Tetradecanoylphorbol Acetate 46-82 gap junction protein alpha 1 Homo sapiens 228-232 8081876-3 1993 EGF-induced phosphorylation and communication inhibition were retained in cells pretreated with phorbol 12-myristate 13-acetate (PMA) to deplete protein kinase C. These results show that the EGF inhibition of communication is tightly linked to protein kinase C-independent phosphorylation of Cx43. Tetradecanoylphorbol Acetate 96-127 gap junction protein alpha 1 Homo sapiens 292-296 11311487-5 2001 Immunostaining of connexin 43 (Cx43) protein in WB cells indicated that TPA caused a loss of Cx43 protein from the cell membranes. Tetradecanoylphorbol Acetate 72-75 gap junction protein alpha 1 Homo sapiens 18-29 11311487-5 2001 Immunostaining of connexin 43 (Cx43) protein in WB cells indicated that TPA caused a loss of Cx43 protein from the cell membranes. Tetradecanoylphorbol Acetate 72-75 gap junction protein alpha 1 Homo sapiens 31-35 11311487-5 2001 Immunostaining of connexin 43 (Cx43) protein in WB cells indicated that TPA caused a loss of Cx43 protein from the cell membranes. Tetradecanoylphorbol Acetate 72-75 gap junction protein alpha 1 Homo sapiens 93-97 11311487-8 2001 The inhibition of GJIC by TPA in WB cells was correlated with the hyperphosphorylation of Cx43 as measured by mobility shifts of the western blot bands of Cx43. Tetradecanoylphorbol Acetate 26-29 gap junction protein alpha 1 Homo sapiens 90-94 11311487-8 2001 The inhibition of GJIC by TPA in WB cells was correlated with the hyperphosphorylation of Cx43 as measured by mobility shifts of the western blot bands of Cx43. Tetradecanoylphorbol Acetate 26-29 gap junction protein alpha 1 Homo sapiens 155-159 11311487-9 2001 TPA induced hyperphosphorylation of Cx43 protein, while GeO(2) appeared to partially block this hyperphosphorylation. Tetradecanoylphorbol Acetate 0-3 gap junction protein alpha 1 Homo sapiens 36-40 12064598-2 2001 Stimulation of cardiomyocytes with phorbol 12-myristate 13-acetate (PMA) increased the fraction of the slower migrating (> or = 45 kDa) and more extensively phosphorylated Cx43 species. Tetradecanoylphorbol Acetate 35-66 gap junction protein alpha 1 Homo sapiens 175-179 12064598-2 2001 Stimulation of cardiomyocytes with phorbol 12-myristate 13-acetate (PMA) increased the fraction of the slower migrating (> or = 45 kDa) and more extensively phosphorylated Cx43 species. Tetradecanoylphorbol Acetate 68-71 gap junction protein alpha 1 Homo sapiens 175-179 12064598-4 2001 Selective inhibition of PKCE significantly decreased baseline levels of Cx43 phosphorylation and the PMA-induced accumulation of > or = 45 kDa Cx43. Tetradecanoylphorbol Acetate 101-104 gap junction protein alpha 1 Homo sapiens 146-150 10434531-4 1999 Our data show increased Cx 43 protein expression in Aroclor 1254 and TPA-treated cultures compared to controls, decreased Cx 43 protein level in those exposed to B[a]P, while Cx 43 gene expression (Cx 43 mRNA) was unaffected by the treatments. Tetradecanoylphorbol Acetate 69-72 gap junction protein alpha 1 Homo sapiens 24-29 10082136-7 1999 Furthermore, pretreatment with hydrogen peroxide (H2O2), a potent MAPK activator but inefficient GJC/Cx43 modulator, abrogated PDGF- or TPA-induced disruption of GJC. Tetradecanoylphorbol Acetate 136-139 gap junction protein alpha 1 Homo sapiens 101-105 10082136-8 1999 While a 5 min H2O2 pretreatment abolished both PDGF- and TPA-induced Cx43 phosphorylation and GJC blockade, a simultaneous H2O2 treatment interfered only with GJC closure but not with the phosphorylation of Cx43 induced by PDGF and TPA. Tetradecanoylphorbol Acetate 57-60 gap junction protein alpha 1 Homo sapiens 69-73 10082136-8 1999 While a 5 min H2O2 pretreatment abolished both PDGF- and TPA-induced Cx43 phosphorylation and GJC blockade, a simultaneous H2O2 treatment interfered only with GJC closure but not with the phosphorylation of Cx43 induced by PDGF and TPA. Tetradecanoylphorbol Acetate 57-60 gap junction protein alpha 1 Homo sapiens 207-211 8071985-9 1994 While TPA treatment induced phosphorylation of connexin43 in these cells, it reduced the expression of connexin45. Tetradecanoylphorbol Acetate 6-9 gap junction protein alpha 1 Homo sapiens 47-57 10471314-4 1999 We show that 13-8300 can detect several phosphorylated species of connexin43 in Western blots after stimulation of two fibroblast cell systems with fresh growth medium, 12-O-tetradecanoyl phorbol-13-acetate, pervanadate, or permolybdate. Tetradecanoylphorbol Acetate 169-206 gap junction protein alpha 1 Homo sapiens 66-76 9791726-3 1998 In the present study it is shown that 12-O-tetradecanoylphorbol-13-acetate (TPA) and EGF induce similar phosphorylation pattern of the gap junction protein connexin43 (Cx43) in K7 cells, although their effects on GJIC are opposite. Tetradecanoylphorbol Acetate 38-74 gap junction protein alpha 1 Homo sapiens 156-166 9791726-3 1998 In the present study it is shown that 12-O-tetradecanoylphorbol-13-acetate (TPA) and EGF induce similar phosphorylation pattern of the gap junction protein connexin43 (Cx43) in K7 cells, although their effects on GJIC are opposite. Tetradecanoylphorbol Acetate 38-74 gap junction protein alpha 1 Homo sapiens 168-172 9791726-3 1998 In the present study it is shown that 12-O-tetradecanoylphorbol-13-acetate (TPA) and EGF induce similar phosphorylation pattern of the gap junction protein connexin43 (Cx43) in K7 cells, although their effects on GJIC are opposite. Tetradecanoylphorbol Acetate 76-79 gap junction protein alpha 1 Homo sapiens 156-166 9791726-3 1998 In the present study it is shown that 12-O-tetradecanoylphorbol-13-acetate (TPA) and EGF induce similar phosphorylation pattern of the gap junction protein connexin43 (Cx43) in K7 cells, although their effects on GJIC are opposite. Tetradecanoylphorbol Acetate 76-79 gap junction protein alpha 1 Homo sapiens 168-172 9648920-4 1998 Down-regulation of PKC-isoforms by 12-O-tetradecanoylphorbol-13-acetate or pretreatment with the PKC inhibitor calphostin C, completely blocked PDGF action on GJC and Cx43. Tetradecanoylphorbol Acetate 35-71 gap junction protein alpha 1 Homo sapiens 167-171 9472478-8 1998 One microM isoproterenol and 5 microM forskolin induced phosphorylation of connexin43, as did 16 nanomolar TPA. Tetradecanoylphorbol Acetate 107-110 gap junction protein alpha 1 Homo sapiens 75-85 8081876-3 1993 EGF-induced phosphorylation and communication inhibition were retained in cells pretreated with phorbol 12-myristate 13-acetate (PMA) to deplete protein kinase C. These results show that the EGF inhibition of communication is tightly linked to protein kinase C-independent phosphorylation of Cx43. Tetradecanoylphorbol Acetate 129-132 gap junction protein alpha 1 Homo sapiens 292-296 8241569-5 1993 EGF in the presence or absence of chronic TPA treatment stimulated marked increases in Cx43 phosphorylation on numerous sites as determined by two-dimensional tryptic phosphopeptide mapping. Tetradecanoylphorbol Acetate 42-45 gap junction protein alpha 1 Homo sapiens 87-91 1654122-3 1991 Longer term incubation with TPA caused loss of connexin 43 protein. Tetradecanoylphorbol Acetate 28-31 gap junction protein alpha 1 Homo sapiens 47-58 8381593-8 1993 At 6 h of treatment, both TPA- and 8-BrcAMP-treated cultures showed markedly elevated levels of connexin43, whereas at 24 h, the level of connexin43 in TPA-treated cultures had returned to control levels. Tetradecanoylphorbol Acetate 26-29 gap junction protein alpha 1 Homo sapiens 96-106 8381593-8 1993 At 6 h of treatment, both TPA- and 8-BrcAMP-treated cultures showed markedly elevated levels of connexin43, whereas at 24 h, the level of connexin43 in TPA-treated cultures had returned to control levels. Tetradecanoylphorbol Acetate 152-155 gap junction protein alpha 1 Homo sapiens 138-148