PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 12766328-2 2003 Using the immortalized GnRH-producing GT1 cell line, we found that activation of protein kinase C (PKC) with 12-O-tetradecanoylphorbol-13-acetate induces morphological and functional differentiation of these neurons. Tetradecanoylphorbol Acetate 109-145 gonadotropin releasing hormone 1 Homo sapiens 23-27 16601289-6 2006 In addition, the activation of ERK1/2 by GnRH-I or II was mimicked by phorbol-12-myristate 13-acetate, a PKC activator. Tetradecanoylphorbol Acetate 70-101 gonadotropin releasing hormone 1 Homo sapiens 41-45 7606799-4 1995 Phorbol ester (PMA), a direct activator of PKC, provoked LHRH production and cell surface expression of CD69 and IL-2R molecules by T cells, but not IL-2 synthesis. Tetradecanoylphorbol Acetate 15-18 gonadotropin releasing hormone 1 Homo sapiens 57-61 9037095-6 1997 The intermediate role of protein kinase C (PKC) in this process was indicated by the ability of phorbol 12-myristate 13-acetate to induce time- and dose-dependent increases in PEt and diacylglycerol, but not inositol trisphosphate, and by reduction of GnRH-induced PEt accumulation in PKC-depleted cells. Tetradecanoylphorbol Acetate 96-127 gonadotropin releasing hormone 1 Homo sapiens 252-256 8793856-4 1996 Reverse transcription-polymerase chain reaction (RT-PCR) analysis was performed to estimate the c-jun and c-fos mRNA contents in GnRH-, forskolin- (cAMP activator) and tetradecanoyl phorbol acetate- (TPA; protein kinase C activator) treated primary cultures of porcine anterior pituitary cells. Tetradecanoylphorbol Acetate 200-203 gonadotropin releasing hormone 1 Homo sapiens 129-133 11159838-5 2001 In addition, pretreatment with a PKC activator, phorbol-12-myristate 13-acetate, potentiated GnRH-induced MAPK activation. Tetradecanoylphorbol Acetate 48-79 gonadotropin releasing hormone 1 Homo sapiens 93-97 9106470-4 1997 In contrast, the PMA-inhibitory effect on GnRH cytoplasmic mRNA levels was significantly reduced or inhibited in the presence of cycloheximide or RNA synthesis inhibitors given within 4 h of PMA, suggesting a protein/RNA synthesis-dependent mechanism for the regulation of GnRH mRNA levels by PMA. Tetradecanoylphorbol Acetate 17-20 gonadotropin releasing hormone 1 Homo sapiens 42-46 9106470-4 1997 In contrast, the PMA-inhibitory effect on GnRH cytoplasmic mRNA levels was significantly reduced or inhibited in the presence of cycloheximide or RNA synthesis inhibitors given within 4 h of PMA, suggesting a protein/RNA synthesis-dependent mechanism for the regulation of GnRH mRNA levels by PMA. Tetradecanoylphorbol Acetate 17-20 gonadotropin releasing hormone 1 Homo sapiens 273-277 9121495-5 1996 TPA stimulation of the human GnRH gene is mediated by a consensus AP-1 site located at -402 to -396 bp, TGACTCA, which specifically binds c-fos and c-jun in Gn11 and NLT cells and recombinant c-jun in gel mobility shift studies. Tetradecanoylphorbol Acetate 0-3 gonadotropin releasing hormone 1 Homo sapiens 29-33 8596491-6 1996 These results suggest that TPA, an activator of PKC, has a stimulatory effect on GRH-induced cAMP production and that, finally, TRH- and CRH-induced PKC activation may cause greater secretion of GH by enhancement of cAMP production in human GH-hypersecreting adenoma cells. Tetradecanoylphorbol Acetate 27-30 gonadotropin releasing hormone 1 Homo sapiens 81-84 7704445-4 1994 Protein kinase C (PKC) depletion induced by a pretreatment with 1 microM PMA for 24 h abolished spontaneous Ca2+ transients and the action of GnRH on [Ca2+]i and Ca2+ channels. Tetradecanoylphorbol Acetate 73-76 gonadotropin releasing hormone 1 Homo sapiens 142-146 7507827-10 1994 Similarly, pretreatment with TPA, but not cAMP or Bay K, prevented subsequent stimulation by GnRH. Tetradecanoylphorbol Acetate 29-32 gonadotropin releasing hormone 1 Homo sapiens 93-97 7518388-3 1994 The agonist-induced expression of PRGs was mimicked by activation of protein kinase-C with the phorbol ester phorbol 12-myristate 13-acetate (PMA), which acted additively with GnRH at low concentrations of both stimuli. Tetradecanoylphorbol Acetate 109-140 gonadotropin releasing hormone 1 Homo sapiens 176-180 7518388-3 1994 The agonist-induced expression of PRGs was mimicked by activation of protein kinase-C with the phorbol ester phorbol 12-myristate 13-acetate (PMA), which acted additively with GnRH at low concentrations of both stimuli. Tetradecanoylphorbol Acetate 142-145 gonadotropin releasing hormone 1 Homo sapiens 176-180 7518388-4 1994 Depletion of cellular protein kinase-C by prior treatment with PMA reduced GnRH- and PMA-induced expression of PRGs. Tetradecanoylphorbol Acetate 63-66 gonadotropin releasing hormone 1 Homo sapiens 75-79 3134022-1 1988 Desensitization of pituitary gonadotropes by exposure to 10 nM gonadotropin-releasing hormone (GnRH) for 6 h severely impaired the luteinizing hormone (LH) response to a second 3-h treatment with GnRH, and reduced the secretory responses to 50 microM arachidonic acid (AA), 100 nM tetradecanoyl phorbol-13-acetate (TPA), and AA + TPA. Tetradecanoylphorbol Acetate 315-318 gonadotropin releasing hormone 1 Homo sapiens 63-93 2643508-2 1989 Treatment of dispersed ovine pituitary cells with the PKC activator phorbol 12-myristate-13-acetate (PMA) for 6 h at a dose of 10 nM stimulated the release of 5- to 10-fold more LH than did GnRH at the same dose. Tetradecanoylphorbol Acetate 68-99 gonadotropin releasing hormone 1 Homo sapiens 190-194 2643508-2 1989 Treatment of dispersed ovine pituitary cells with the PKC activator phorbol 12-myristate-13-acetate (PMA) for 6 h at a dose of 10 nM stimulated the release of 5- to 10-fold more LH than did GnRH at the same dose. Tetradecanoylphorbol Acetate 101-104 gonadotropin releasing hormone 1 Homo sapiens 190-194 3053708-8 1988 TPA caused rapid and complete translocation of cytosolic protein kinase C to the particulate fraction of pituitary cells, followed by a progressive decrease in total enzyme content to approximately 10% after 6 h. Partial recovery of the cytosolic enzyme (to 20%) occurred after washing and reincubation for 15 h. Such kinase C-depleted cells showed prominent, dose-dependent reductions in the actions of GnRH and TPA on LH release and synthesis in both normal and Ca2+-deficient media. Tetradecanoylphorbol Acetate 0-3 gonadotropin releasing hormone 1 Homo sapiens 404-408 3134022-1 1988 Desensitization of pituitary gonadotropes by exposure to 10 nM gonadotropin-releasing hormone (GnRH) for 6 h severely impaired the luteinizing hormone (LH) response to a second 3-h treatment with GnRH, and reduced the secretory responses to 50 microM arachidonic acid (AA), 100 nM tetradecanoyl phorbol-13-acetate (TPA), and AA + TPA. Tetradecanoylphorbol Acetate 315-318 gonadotropin releasing hormone 1 Homo sapiens 95-99 3134022-1 1988 Desensitization of pituitary gonadotropes by exposure to 10 nM gonadotropin-releasing hormone (GnRH) for 6 h severely impaired the luteinizing hormone (LH) response to a second 3-h treatment with GnRH, and reduced the secretory responses to 50 microM arachidonic acid (AA), 100 nM tetradecanoyl phorbol-13-acetate (TPA), and AA + TPA. Tetradecanoylphorbol Acetate 315-318 gonadotropin releasing hormone 1 Homo sapiens 196-200 3134022-1 1988 Desensitization of pituitary gonadotropes by exposure to 10 nM gonadotropin-releasing hormone (GnRH) for 6 h severely impaired the luteinizing hormone (LH) response to a second 3-h treatment with GnRH, and reduced the secretory responses to 50 microM arachidonic acid (AA), 100 nM tetradecanoyl phorbol-13-acetate (TPA), and AA + TPA. Tetradecanoylphorbol Acetate 330-333 gonadotropin releasing hormone 1 Homo sapiens 63-93 3134022-1 1988 Desensitization of pituitary gonadotropes by exposure to 10 nM gonadotropin-releasing hormone (GnRH) for 6 h severely impaired the luteinizing hormone (LH) response to a second 3-h treatment with GnRH, and reduced the secretory responses to 50 microM arachidonic acid (AA), 100 nM tetradecanoyl phorbol-13-acetate (TPA), and AA + TPA. Tetradecanoylphorbol Acetate 330-333 gonadotropin releasing hormone 1 Homo sapiens 95-99 3134022-1 1988 Desensitization of pituitary gonadotropes by exposure to 10 nM gonadotropin-releasing hormone (GnRH) for 6 h severely impaired the luteinizing hormone (LH) response to a second 3-h treatment with GnRH, and reduced the secretory responses to 50 microM arachidonic acid (AA), 100 nM tetradecanoyl phorbol-13-acetate (TPA), and AA + TPA. Tetradecanoylphorbol Acetate 330-333 gonadotropin releasing hormone 1 Homo sapiens 196-200 2825684-2 1987 Addition of the tumor promoter phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA), at concentrations which activate pituitary protein C kinase, to cultured pituitary cells resulted in up-regulation of GnRH receptors (155% at 4 h). Tetradecanoylphorbol Acetate 45-81 gonadotropin releasing hormone 1 Homo sapiens 207-211 2825684-2 1987 Addition of the tumor promoter phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA), at concentrations which activate pituitary protein C kinase, to cultured pituitary cells resulted in up-regulation of GnRH receptors (155% at 4 h). Tetradecanoylphorbol Acetate 83-86 gonadotropin releasing hormone 1 Homo sapiens 207-211 2825684-3 1987 The stimulatory effect of GnRH on [3H]inositol phosphates (Ins-P) production in myo-[2-3H]inositol prelabeled pituitary cells was not inhibited by prior treatment of the cells with TPA (10(-9)-10(-7) M). Tetradecanoylphorbol Acetate 181-184 gonadotropin releasing hormone 1 Homo sapiens 26-30 2825684-4 1987 Higher concentrations of TPA (10(-6)-10(-5) M) inhibited the effect of GnRH on [3H]Ins-P production. Tetradecanoylphorbol Acetate 25-28 gonadotropin releasing hormone 1 Homo sapiens 71-75 3104328-4 1987 Further, phorbol ester- and diacylglycerol-stimulated LH release, as well as inhibition by PMA of gonadotropin-releasing hormone (GnRH)-stimulated inositol phosphate production, were reduced by pretreatment with PMA. Tetradecanoylphorbol Acetate 91-94 gonadotropin releasing hormone 1 Homo sapiens 98-128 3161505-3 1985 Simultaneous presence of A23187 and OAG or TPA resulted in a synergistic response that mimicked the full physiological response to gonadotropin releasing hormone (GnRH). Tetradecanoylphorbol Acetate 43-46 gonadotropin releasing hormone 1 Homo sapiens 131-161 3161505-3 1985 Simultaneous presence of A23187 and OAG or TPA resulted in a synergistic response that mimicked the full physiological response to gonadotropin releasing hormone (GnRH). Tetradecanoylphorbol Acetate 43-46 gonadotropin releasing hormone 1 Homo sapiens 163-167