PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 9593123-1 1998 A TEM-1 beta-lactamase derivative containing the single amino acid substitution A237T slightly increased (from 24 to 32 microg/ml) the cephalothin MIC for Escherichia coli RYC1000 but did not influence the activities of cefotaxime, ceftazidime, and aztreonam (MICs of 0.03, 0.12, and 0.06 microg/ml, respectively). Aztreonam 249-258 TEM-1 beta-lactamase Escherichia coli 2-22 12909725-7 2003 We demonstrate that our error-prone Pol I can be used to generate enzymes with distinct properties by generating TEM-1 beta-lactamase mutants able to hydrolyze a third-generation lactam antibiotic, aztreonam. Aztreonam 198-207 TEM-1 beta-lactamase Escherichia coli 113-133