PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 20926293-3 2010 HTMC treatment also led to an inhibition of cell-cycle regulatory proteins phosphorylation of cdc2 (Tyr(15) and Tyr(161)) and Rb (Ser(795) and Ser(807/811)), which was accompanied by the accumulation of tumor suppressor genes p53 and p21. Tyrosine 100-103 cyclin dependent kinase 1 Homo sapiens 94-98 22084170-7 2012 In MK1775-treated cells, CDC2 activity was enhanced, as determined by decreased inhibitory phosphorylation of tyrosine-15 residue and increased expression of phosphorylated histone H3, a marker of mitotic entry. Tyrosine 110-118 cyclin dependent kinase 1 Homo sapiens 25-29 22204398-5 2011 In the presence of WIN55212-2, cdc2 protein and mRNA expression was progressively enhanced and Tyr-15-phosporylation of cdc2 was reduced in differentiating U937 cells. Tyrosine 95-98 cyclin dependent kinase 1 Homo sapiens 120-124 21734451-5 2011 It can trigger G 2/M arrest in wild type p53 containing cells, which was attributed to the decreased Cdc2 kinase activity resulting at least partly from a high level of inhibitory tyrosine phosphorylation on Cdc2 protein at Tyr-15. Tyrosine 180-188 cyclin dependent kinase 1 Homo sapiens 101-105 21734451-5 2011 It can trigger G 2/M arrest in wild type p53 containing cells, which was attributed to the decreased Cdc2 kinase activity resulting at least partly from a high level of inhibitory tyrosine phosphorylation on Cdc2 protein at Tyr-15. Tyrosine 180-188 cyclin dependent kinase 1 Homo sapiens 208-212 21734451-5 2011 It can trigger G 2/M arrest in wild type p53 containing cells, which was attributed to the decreased Cdc2 kinase activity resulting at least partly from a high level of inhibitory tyrosine phosphorylation on Cdc2 protein at Tyr-15. Tyrosine 224-227 cyclin dependent kinase 1 Homo sapiens 101-105 21734451-5 2011 It can trigger G 2/M arrest in wild type p53 containing cells, which was attributed to the decreased Cdc2 kinase activity resulting at least partly from a high level of inhibitory tyrosine phosphorylation on Cdc2 protein at Tyr-15. Tyrosine 224-227 cyclin dependent kinase 1 Homo sapiens 208-212 21321122-9 2011 The elevated cdc2 had inactivating phosphorylation on Thr(14) or Tyr(15), and possibly both, due to an increase in the responsible Wee1 and Myt1 kinases. Tyrosine 65-68 cyclin dependent kinase 1 Homo sapiens 13-17 22012316-2 2012 The results demonstrate that all of the three oxoanions can cause G(2)/M cell cycle arrest, which is evidenced by the increase in the level of phosphorylated Cdc2 at its inactive Tyr-15 site. Tyrosine 179-182 cyclin dependent kinase 1 Homo sapiens 158-162 21900237-0 2011 CDK1-mediated phosphorylation of Abi1 attenuates Bcr-Abl-induced F-actin assembly and tyrosine phosphorylation of WAVE complex during mitosis. Tyrosine 86-94 cyclin dependent kinase 1 Homo sapiens 0-4 20840867-4 2011 While HU potently induced CDC2 Y15 (tyrosine 15) phosphorylation, an event causing CDC2 inactivation, inhibition of ERK kinases using U0126 (a MEK inhibitor), MEK1K97M (a dominant negative MEK1), and knockdown of either ERK1 or ERK2 significantly attenuated HU-induced CDC2 Y15 phosphorylation. Tyrosine 36-44 cyclin dependent kinase 1 Homo sapiens 26-30 20406171-4 2010 Wee 1 is a tyrosine kinase that phosphorylates CDC2 at Tyr 15 and as such plays a pivotal role in the G2 DNA damage checkpoint. Tyrosine 55-58 cyclin dependent kinase 1 Homo sapiens 47-51 22993588-8 2010 Treatment of cells with DA 3003-2 caused hyperphosphorylation of Cdc2 tyr(15) in cyclin B(1) and cyclin A complexes. Tyrosine 70-73 cyclin dependent kinase 1 Homo sapiens 65-69 19795419-8 2010 The latter correlated well with upregulation of cyclin D3 and p21(Waf1/Cip1) as well as with inhibition of cdc2 activity through Tyr-15 phosphorylation. Tyrosine 129-132 cyclin dependent kinase 1 Homo sapiens 107-111 20395957-0 2010 New Cdc2 Tyr 4 phosphorylation by dsRNA-activated protein kinase triggers Cdc2 polyubiquitination and G2 arrest under genotoxic stresses. Tyrosine 9-12 cyclin dependent kinase 1 Homo sapiens 4-8 20395957-0 2010 New Cdc2 Tyr 4 phosphorylation by dsRNA-activated protein kinase triggers Cdc2 polyubiquitination and G2 arrest under genotoxic stresses. Tyrosine 9-12 cyclin dependent kinase 1 Homo sapiens 74-78 20395957-5 2010 Phospho-mimic mutations at the Tyr 4 residue (Y4D or Y4E) caused significant ubiquitination of Cdc2 even in the absence of PKR. Tyrosine 31-34 cyclin dependent kinase 1 Homo sapiens 95-99 20395957-6 2010 Our findings demonstrate that (i) PKR, Ser/Thr kinase, phosphorylates its new substrate Cdc2 at the Tyr 4 residue, (ii) PKR-mediated Tyr 4-phosphorylation facilitates Cdc2 ubiquitination and proteosomal degradation, (iii) unphosphorylated Tyr 4 prevents Cdc2 ubiquitination, and (iv) downstream from p53, PKR has a crucial role in G2 arrest and triggers Cdc2 downregulation under genotoxic conditions. Tyrosine 100-103 cyclin dependent kinase 1 Homo sapiens 88-92 20395957-6 2010 Our findings demonstrate that (i) PKR, Ser/Thr kinase, phosphorylates its new substrate Cdc2 at the Tyr 4 residue, (ii) PKR-mediated Tyr 4-phosphorylation facilitates Cdc2 ubiquitination and proteosomal degradation, (iii) unphosphorylated Tyr 4 prevents Cdc2 ubiquitination, and (iv) downstream from p53, PKR has a crucial role in G2 arrest and triggers Cdc2 downregulation under genotoxic conditions. Tyrosine 100-103 cyclin dependent kinase 1 Homo sapiens 167-171 20395957-6 2010 Our findings demonstrate that (i) PKR, Ser/Thr kinase, phosphorylates its new substrate Cdc2 at the Tyr 4 residue, (ii) PKR-mediated Tyr 4-phosphorylation facilitates Cdc2 ubiquitination and proteosomal degradation, (iii) unphosphorylated Tyr 4 prevents Cdc2 ubiquitination, and (iv) downstream from p53, PKR has a crucial role in G2 arrest and triggers Cdc2 downregulation under genotoxic conditions. Tyrosine 100-103 cyclin dependent kinase 1 Homo sapiens 167-171 20395957-6 2010 Our findings demonstrate that (i) PKR, Ser/Thr kinase, phosphorylates its new substrate Cdc2 at the Tyr 4 residue, (ii) PKR-mediated Tyr 4-phosphorylation facilitates Cdc2 ubiquitination and proteosomal degradation, (iii) unphosphorylated Tyr 4 prevents Cdc2 ubiquitination, and (iv) downstream from p53, PKR has a crucial role in G2 arrest and triggers Cdc2 downregulation under genotoxic conditions. Tyrosine 100-103 cyclin dependent kinase 1 Homo sapiens 167-171 20395957-6 2010 Our findings demonstrate that (i) PKR, Ser/Thr kinase, phosphorylates its new substrate Cdc2 at the Tyr 4 residue, (ii) PKR-mediated Tyr 4-phosphorylation facilitates Cdc2 ubiquitination and proteosomal degradation, (iii) unphosphorylated Tyr 4 prevents Cdc2 ubiquitination, and (iv) downstream from p53, PKR has a crucial role in G2 arrest and triggers Cdc2 downregulation under genotoxic conditions. Tyrosine 133-136 cyclin dependent kinase 1 Homo sapiens 88-92 20395957-6 2010 Our findings demonstrate that (i) PKR, Ser/Thr kinase, phosphorylates its new substrate Cdc2 at the Tyr 4 residue, (ii) PKR-mediated Tyr 4-phosphorylation facilitates Cdc2 ubiquitination and proteosomal degradation, (iii) unphosphorylated Tyr 4 prevents Cdc2 ubiquitination, and (iv) downstream from p53, PKR has a crucial role in G2 arrest and triggers Cdc2 downregulation under genotoxic conditions. Tyrosine 133-136 cyclin dependent kinase 1 Homo sapiens 167-171 20395957-6 2010 Our findings demonstrate that (i) PKR, Ser/Thr kinase, phosphorylates its new substrate Cdc2 at the Tyr 4 residue, (ii) PKR-mediated Tyr 4-phosphorylation facilitates Cdc2 ubiquitination and proteosomal degradation, (iii) unphosphorylated Tyr 4 prevents Cdc2 ubiquitination, and (iv) downstream from p53, PKR has a crucial role in G2 arrest and triggers Cdc2 downregulation under genotoxic conditions. Tyrosine 133-136 cyclin dependent kinase 1 Homo sapiens 167-171 20395957-6 2010 Our findings demonstrate that (i) PKR, Ser/Thr kinase, phosphorylates its new substrate Cdc2 at the Tyr 4 residue, (ii) PKR-mediated Tyr 4-phosphorylation facilitates Cdc2 ubiquitination and proteosomal degradation, (iii) unphosphorylated Tyr 4 prevents Cdc2 ubiquitination, and (iv) downstream from p53, PKR has a crucial role in G2 arrest and triggers Cdc2 downregulation under genotoxic conditions. Tyrosine 133-136 cyclin dependent kinase 1 Homo sapiens 167-171 20395957-6 2010 Our findings demonstrate that (i) PKR, Ser/Thr kinase, phosphorylates its new substrate Cdc2 at the Tyr 4 residue, (ii) PKR-mediated Tyr 4-phosphorylation facilitates Cdc2 ubiquitination and proteosomal degradation, (iii) unphosphorylated Tyr 4 prevents Cdc2 ubiquitination, and (iv) downstream from p53, PKR has a crucial role in G2 arrest and triggers Cdc2 downregulation under genotoxic conditions. Tyrosine 133-136 cyclin dependent kinase 1 Homo sapiens 88-92 20395957-6 2010 Our findings demonstrate that (i) PKR, Ser/Thr kinase, phosphorylates its new substrate Cdc2 at the Tyr 4 residue, (ii) PKR-mediated Tyr 4-phosphorylation facilitates Cdc2 ubiquitination and proteosomal degradation, (iii) unphosphorylated Tyr 4 prevents Cdc2 ubiquitination, and (iv) downstream from p53, PKR has a crucial role in G2 arrest and triggers Cdc2 downregulation under genotoxic conditions. Tyrosine 133-136 cyclin dependent kinase 1 Homo sapiens 167-171 20395957-6 2010 Our findings demonstrate that (i) PKR, Ser/Thr kinase, phosphorylates its new substrate Cdc2 at the Tyr 4 residue, (ii) PKR-mediated Tyr 4-phosphorylation facilitates Cdc2 ubiquitination and proteosomal degradation, (iii) unphosphorylated Tyr 4 prevents Cdc2 ubiquitination, and (iv) downstream from p53, PKR has a crucial role in G2 arrest and triggers Cdc2 downregulation under genotoxic conditions. Tyrosine 133-136 cyclin dependent kinase 1 Homo sapiens 167-171 20395957-6 2010 Our findings demonstrate that (i) PKR, Ser/Thr kinase, phosphorylates its new substrate Cdc2 at the Tyr 4 residue, (ii) PKR-mediated Tyr 4-phosphorylation facilitates Cdc2 ubiquitination and proteosomal degradation, (iii) unphosphorylated Tyr 4 prevents Cdc2 ubiquitination, and (iv) downstream from p53, PKR has a crucial role in G2 arrest and triggers Cdc2 downregulation under genotoxic conditions. Tyrosine 133-136 cyclin dependent kinase 1 Homo sapiens 167-171 20368335-1 2010 Cdc25A is a dual specificity protein phosphatase that activates cyclin/cyclin-dependent protein kinase (Cdk) complexes by removing inhibitory phosphates from conserved threonine and tyrosine in Cdks. Tyrosine 182-190 cyclin dependent kinase 1 Homo sapiens 104-107 20368335-1 2010 Cdc25A is a dual specificity protein phosphatase that activates cyclin/cyclin-dependent protein kinase (Cdk) complexes by removing inhibitory phosphates from conserved threonine and tyrosine in Cdks. Tyrosine 182-190 cyclin dependent kinase 1 Homo sapiens 194-198 20360007-2 2010 Activation of Cdk1 is a multistep process comprising binding of Cdk1 to cyclin B, relocation of cyclin-kinase complexes to the nucleus, activating phosphorylation of Cdk1 on Thr(161) by the Cdk-activating kinase (CAK; Cdk7 in metazoans), and removal of inhibitory Thr(14) and Tyr(15) phosphorylations. Tyrosine 276-279 cyclin dependent kinase 1 Homo sapiens 14-18 20360007-5 2010 In Cdc25A-overexpressing cells, Cdk1 exhibits high kinase activity despite being phosphorylated on Tyr(15). Tyrosine 99-102 cyclin dependent kinase 1 Homo sapiens 32-36 20360007-6 2010 In addition, Tyr(15)-phosphorylated Cdk1 binds more cyclin B in Cdc25A-overexpressing cells compared with control cells. Tyrosine 13-16 cyclin dependent kinase 1 Homo sapiens 36-40 19858290-2 2010 Inactivating human cyclin A/Cdk2 complexes through diverse approaches delays mitotic entry and promotes inhibitory phosphorylation of Cdk1 on tyrosine 15, a modification performed by Wee1. Tyrosine 142-150 cyclin dependent kinase 1 Homo sapiens 134-138 19917613-4 2010 Consistent with a G(2)/M arrest, PKCdelta-cat induced phosphorylation of Cdk1 (Tyr(15)), a critical event in the G(2)/M checkpoint. Tyrosine 79-82 cyclin dependent kinase 1 Homo sapiens 73-77 19527713-4 2009 During bleomycin-induced over-replication, mitotic entry is inhibited through tyrosine phosphorylation of CDK1 along the ATM/ATR pathway in the early phase of treatment. Tyrosine 78-86 cyclin dependent kinase 1 Homo sapiens 106-110 19530246-4 2009 This resulted in Cdk1 tyrosine phosphorylation at Tyr-15, leading to cell cycle block at G2/M phase. Tyrosine 22-30 cyclin dependent kinase 1 Homo sapiens 17-21 19530246-4 2009 This resulted in Cdk1 tyrosine phosphorylation at Tyr-15, leading to cell cycle block at G2/M phase. Tyrosine 50-53 cyclin dependent kinase 1 Homo sapiens 17-21 18817634-5 2008 Furthermore, LGH00045 impaired the proliferation of tumor cells and increased cyclin-dependent kinase 1 inhibitory tyrosine phosphorylation. Tyrosine 115-123 cyclin dependent kinase 1 Homo sapiens 78-103 19730430-5 2009 LGH00031 increased cyclin-dependent kinase 1 (CDK1) tyrosine 15 phosphorylation and cyclin B1 protein level. Tyrosine 52-60 cyclin dependent kinase 1 Homo sapiens 19-44 19730430-5 2009 LGH00031 increased cyclin-dependent kinase 1 (CDK1) tyrosine 15 phosphorylation and cyclin B1 protein level. Tyrosine 52-60 cyclin dependent kinase 1 Homo sapiens 46-50 19730430-9 2009 Correspondingly, the LGH00031-induced decrease in cell viability and cell cycle arrest, cyclin B1 protein level, and phosphorylation of CDK1 tyrosine 15 were also rescued by NAC that decreased ROS production. Tyrosine 141-149 cyclin dependent kinase 1 Homo sapiens 136-140 18479465-3 2008 This site is equivalent to tyrosine 15 of cyclin dependent kinase 1, which undergoes inhibitory phosphorylation by WEE1 and MYT1; however, the kinases that phosphorylate cyclin dependent kinase 4 on tyrosine 17 are still unknown. Tyrosine 27-35 cyclin dependent kinase 1 Homo sapiens 42-67 18247328-2 2008 In this article, we demonstrated that lithium arrested hepatocellular carcinoma cell SMMC-7721 at G2/M checkpoint by inducing the phosphorylation of cdc2 (Tyr-15). Tyrosine 155-158 cyclin dependent kinase 1 Homo sapiens 149-153 18247328-9 2008 Moreover, caffeine, a known ATM/ATR kinase inhibitor, relieved the phosphorylation of cdc2 (Tyr-15) by hydroxyurea, but not that by lithium. Tyrosine 92-95 cyclin dependent kinase 1 Homo sapiens 86-90 18479465-3 2008 This site is equivalent to tyrosine 15 of cyclin dependent kinase 1, which undergoes inhibitory phosphorylation by WEE1 and MYT1; however, the kinases that phosphorylate cyclin dependent kinase 4 on tyrosine 17 are still unknown. Tyrosine 199-207 cyclin dependent kinase 1 Homo sapiens 42-67 17095507-1 2007 Inhibition of cyclin-dependent kinase 1 (CDK1) activity by Tyr-15 phosphorylation directly regulates entry into mitosis and is an important element in the control of the unperturbed cell cycle. Tyrosine 59-62 cyclin dependent kinase 1 Homo sapiens 14-39 17619824-11 2007 Coincident with G2M arrest, phosphorylation of CDC2 on Tyr-15 was significantly elevated, which could be explained by the increase of Wee1 and decrease of CDC25C. Tyrosine 55-58 cyclin dependent kinase 1 Homo sapiens 47-51 17989878-6 2007 The accumulation of cyclinB1 protein and the suppression of Cdc2 tyrosine-15 and Cdc25C serine-216 phosphorylation were detected in irradiated cells treated with tetrandrine using Western blot analysis. Tyrosine 65-73 cyclin dependent kinase 1 Homo sapiens 60-64 17636382-7 2007 Cdk1 tyrosine 15 phosphorylation was associated with G2M arrest, but not with cell death. Tyrosine 5-13 cyclin dependent kinase 1 Homo sapiens 0-4 17636382-8 2007 Cdk1 tyrosine 15 phosphorylation also led to suppression of nuclear cyclin B-associated Cdk1 kinase activity. Tyrosine 5-13 cyclin dependent kinase 1 Homo sapiens 0-4 17636382-8 2007 Cdk1 tyrosine 15 phosphorylation also led to suppression of nuclear cyclin B-associated Cdk1 kinase activity. Tyrosine 5-13 cyclin dependent kinase 1 Homo sapiens 88-92 17636382-9 2007 However cell death, associated with broader tyrosine phosphorylation of Cdk1 was not attributed to tyrosine 15 alone. Tyrosine 44-52 cyclin dependent kinase 1 Homo sapiens 72-76 17869387-6 2008 HT-29 cells treated with doxorubicin and then target compounds demonstrate an active Cdc2/cyclin B complex, inhibition of the doxorubicin-induced phosphorylation of tyrosine 15 of Cdc2 and abrogation of the G2 checkpoint. Tyrosine 165-173 cyclin dependent kinase 1 Homo sapiens 85-89 17869387-6 2008 HT-29 cells treated with doxorubicin and then target compounds demonstrate an active Cdc2/cyclin B complex, inhibition of the doxorubicin-induced phosphorylation of tyrosine 15 of Cdc2 and abrogation of the G2 checkpoint. Tyrosine 165-173 cyclin dependent kinase 1 Homo sapiens 180-184 18225552-9 2007 Phosphorylation of Cdc2 at residue tyrosine-15 was also induced by Asc-S treatment. Tyrosine 35-43 cyclin dependent kinase 1 Homo sapiens 19-23 17475221-10 2007 The exposure of cells to 2-phenyl-4-quinolone caused Cdk1 activation by several observations, namely (i) elevation of cyclin B1 expression, (ii) dephosphorylation on inhibitory Tyr-15 of Cdk1, and (iii) dephosphorylation on Ser-216 of Cdc25c. Tyrosine 177-180 cyclin dependent kinase 1 Homo sapiens 53-57 17412972-4 2007 To characterize the mechanism further, Western blots of ORF20-recombinant lentivirus-infected cells were developed with antibodies to cyclin B1, Cdc2 and phospho-Tyr-15-Cdc2. Tyrosine 162-165 cyclin dependent kinase 1 Homo sapiens 169-173 17412972-5 2007 This analysis revealed a relative increase in cyclin B and phospho-Tyr-15-Cdc2, from 24 to 72 h after infection with recombinant lentivirus. Tyrosine 67-70 cyclin dependent kinase 1 Homo sapiens 74-78 17210576-3 2007 A requirement for PP2A by Vpr was first shown with the PP2A-specific inhibitor okadaic acid, which reduced Vpr-induced G(2) arrest and Cdk1-Tyr(15) phosphorylation. Tyrosine 140-143 cyclin dependent kinase 1 Homo sapiens 135-139 17095507-1 2007 Inhibition of cyclin-dependent kinase 1 (CDK1) activity by Tyr-15 phosphorylation directly regulates entry into mitosis and is an important element in the control of the unperturbed cell cycle. Tyrosine 59-62 cyclin dependent kinase 1 Homo sapiens 41-45 17172433-4 2006 Additional mechanistic studies showed that gallic acid induces an early Tyr(15) phosphorylation of cell division cycle 2 (cdc2). Tyrosine 72-75 cyclin dependent kinase 1 Homo sapiens 99-120 17172433-4 2006 Additional mechanistic studies showed that gallic acid induces an early Tyr(15) phosphorylation of cell division cycle 2 (cdc2). Tyrosine 72-75 cyclin dependent kinase 1 Homo sapiens 122-126 17013563-5 2006 Furthermore, cyclin B1 and CDK1 were detected co-localizing and associating in G1 phase MOLT-4 cells, with the cellular lysates from these cells revealing a genotoxic stress-induced increase in CDK1 phosphorylation (Thr-161) and dephosphorylation (Tyr-15), as analyzed by postsorting immunoprecipitation and immunoblotting. Tyrosine 248-251 cyclin dependent kinase 1 Homo sapiens 27-31 17013563-5 2006 Furthermore, cyclin B1 and CDK1 were detected co-localizing and associating in G1 phase MOLT-4 cells, with the cellular lysates from these cells revealing a genotoxic stress-induced increase in CDK1 phosphorylation (Thr-161) and dephosphorylation (Tyr-15), as analyzed by postsorting immunoprecipitation and immunoblotting. Tyrosine 248-251 cyclin dependent kinase 1 Homo sapiens 194-198 16840322-5 2006 Inactivation of cdk1 is through inhibitory Tyr(15) phosphorylation, with cells containing elevated levels of Wee1, the kinase responsible for inhibitory cdk1 phosphorylation. Tyrosine 43-46 cyclin dependent kinase 1 Homo sapiens 16-20 16807237-2 2006 Cdc25C activates Cdc2 for entry into mitosis by dephosphorylating Thr and Tyr at the site of inhibitory phosphorylation. Tyrosine 74-77 cyclin dependent kinase 1 Homo sapiens 0-4 15615778-3 2005 The p53-independent pathway involves a phosphorylation cascade that activates the Chk1 effector kinase and induces G2 arrest through inhibitory tyrosine phosphorylation of Cdc2. Tyrosine 144-152 cyclin dependent kinase 1 Homo sapiens 172-176 16783362-8 2006 In contrast with ATR-Seckel syndrome cells, MCPH1-mutant cells have low levels of Tyr 15-phosphorylated Cdk1 (pY15-Cdk1) in S and G2 phases, which correlates with an elevated frequency of G2-like cells displaying premature chromosome condensation (PCC). Tyrosine 82-85 cyclin dependent kinase 1 Homo sapiens 104-108 16783362-8 2006 In contrast with ATR-Seckel syndrome cells, MCPH1-mutant cells have low levels of Tyr 15-phosphorylated Cdk1 (pY15-Cdk1) in S and G2 phases, which correlates with an elevated frequency of G2-like cells displaying premature chromosome condensation (PCC). Tyrosine 82-85 cyclin dependent kinase 1 Homo sapiens 110-119 16481012-9 2006 As a consequence, tyrosine 15 (Y15) on Cdc2 remains hypophosphorylated, and there is a loss of the G2/M checkpoint. Tyrosine 18-26 cyclin dependent kinase 1 Homo sapiens 39-43 16619491-7 2006 The expression of cdc2 was not changed and phospho-cdc2 (Tyr 15) increased after 36-, 48- and 60-h treatment. Tyrosine 57-60 cyclin dependent kinase 1 Homo sapiens 51-55 15780175-2 2005 In both human and fission yeast (Schizosaccharomyces pombe) cells, the activity of Cdc2 is regulated in part by the phosphorylation status of tyrosine 15 (Tyr15) on Cdc2, which is phosphorylated by Wee1 kinase during late G2 and is rapidly dephosphorylated by the Cdc25 tyrosine phosphatase to trigger entry into mitosis. Tyrosine 142-150 cyclin dependent kinase 1 Homo sapiens 83-87 15780175-2 2005 In both human and fission yeast (Schizosaccharomyces pombe) cells, the activity of Cdc2 is regulated in part by the phosphorylation status of tyrosine 15 (Tyr15) on Cdc2, which is phosphorylated by Wee1 kinase during late G2 and is rapidly dephosphorylated by the Cdc25 tyrosine phosphatase to trigger entry into mitosis. Tyrosine 142-150 cyclin dependent kinase 1 Homo sapiens 165-169 16614167-8 2006 WEE1 and p21CIP1/WAF1 inhibit cyclin B/CDC2 by CDC2 tyrosine-15 phosphorylation and direct binding, respectively. Tyrosine 52-60 cyclin dependent kinase 1 Homo sapiens 39-43 16614167-8 2006 WEE1 and p21CIP1/WAF1 inhibit cyclin B/CDC2 by CDC2 tyrosine-15 phosphorylation and direct binding, respectively. Tyrosine 52-60 cyclin dependent kinase 1 Homo sapiens 47-51 16546994-4 2006 Inhibitory phosphorylation at Thr(14) and Tyr(15) of the CDKs is modulated by complex regulatory mechanisms involving multiple kinases and phosphatases. Tyrosine 42-45 cyclin dependent kinase 1 Homo sapiens 57-61 15940258-3 2005 The DATS-induced cell cycle arrest in PC-3 cells was associated with increased Tyr(15) phosphorylation of cyclin-dependent kinase 1 (Cdk 1) and inhibition of Cdk 1/cyclinB 1 kinase activity. Tyrosine 79-82 cyclin dependent kinase 1 Homo sapiens 106-131 15940258-3 2005 The DATS-induced cell cycle arrest in PC-3 cells was associated with increased Tyr(15) phosphorylation of cyclin-dependent kinase 1 (Cdk 1) and inhibition of Cdk 1/cyclinB 1 kinase activity. Tyrosine 79-82 cyclin dependent kinase 1 Homo sapiens 133-138 15582998-6 2005 The p14(ARF)-induced depletion of p34(cdc2) was associated with impaired cdc25C phosphatase expression and a prominent shift to inhibitory Tyr-15-phosphorylation in G(2)-arrested cells lacking either p53, p21, or both. Tyrosine 139-142 cyclin dependent kinase 1 Homo sapiens 38-42 15208305-5 2004 Activation of the Ras-Ral signaling affected neither the level nor the intracellular localization of cyclin B1 and CDC2 but interfered with the CDC2 inhibitory phosphorylation at Tyr(15) and the decrease in the cyclin B/CDC2 kinase activity in damaged cells. Tyrosine 179-182 cyclin dependent kinase 1 Homo sapiens 144-148 15576941-2 2005 They function by phosphorylating Cdc2 and related Cdks on conserved tyrosine and threonine residues. Tyrosine 68-76 cyclin dependent kinase 1 Homo sapiens 33-37 15576941-2 2005 They function by phosphorylating Cdc2 and related Cdks on conserved tyrosine and threonine residues. Tyrosine 68-76 cyclin dependent kinase 1 Homo sapiens 50-54 15208305-5 2004 Activation of the Ras-Ral signaling affected neither the level nor the intracellular localization of cyclin B1 and CDC2 but interfered with the CDC2 inhibitory phosphorylation at Tyr(15) and the decrease in the cyclin B/CDC2 kinase activity in damaged cells. Tyrosine 179-182 cyclin dependent kinase 1 Homo sapiens 144-148 15126376-5 2004 It impairs the proliferation of tumoral cell lines and increases cyclin-dependent kinase 1 inhibitory tyrosine phosphorylation. Tyrosine 102-110 cyclin dependent kinase 1 Homo sapiens 65-90 15136770-6 2004 Treatment of cells with the S-phase blocker PD179483 causes abnormal and persistent hyperactivation of Cdk2 and Cdc2 due to Tyr-15 dephosphorylation. Tyrosine 124-127 cyclin dependent kinase 1 Homo sapiens 112-116 14681206-3 2003 Cdc25A is required for S-phase entry and dephosphorylates tyrosine-15 phosphorylated Cdk1 (Cdc2) and Cdk2, positive regulators of cell division. Tyrosine 58-66 cyclin dependent kinase 1 Homo sapiens 85-89 14968113-2 2004 Activation occurs by dephosphorylation of the inhibitory residues, threonine 14 (T14) and tyrosine 15 (Y15), on Cdc2 by the Cdc25C phosphatase. Tyrosine 90-98 cyclin dependent kinase 1 Homo sapiens 112-116 14767478-4 2004 We report here that reducing cyclin A expression by RNA interference (RNAi) in primary human fibroblasts inhibited activation of Cdc25B and Cdc25C and dephosphorylation of Cdk1 on tyrosine (tyr) 15. Tyrosine 180-188 cyclin dependent kinase 1 Homo sapiens 172-176 14767478-4 2004 We report here that reducing cyclin A expression by RNA interference (RNAi) in primary human fibroblasts inhibited activation of Cdc25B and Cdc25C and dephosphorylation of Cdk1 on tyrosine (tyr) 15. Tyrosine 180-183 cyclin dependent kinase 1 Homo sapiens 172-176 14767478-6 2004 Cdk2-dn induction could inhibit Cdc25B activity and foster Cdk1 tyr phosphorylation within the S phase, temporally dissociating these events from Cdk1 activation at mitosis. Tyrosine 64-67 cyclin dependent kinase 1 Homo sapiens 59-63 14681206-3 2003 Cdc25A is required for S-phase entry and dephosphorylates tyrosine-15 phosphorylated Cdk1 (Cdc2) and Cdk2, positive regulators of cell division. Tyrosine 58-66 cyclin dependent kinase 1 Homo sapiens 0-4 12472573-4 2002 There was no predicted cell cycle arrest in Bcr-abl-positive derivative cell lines of K562 that did not survive the same genotoxic insult but, paradoxically, Cdk1 tyrosine phosphorylation was enhanced to a higher extent compared with the parental cell line where arrest of the cell cycle was observed. Tyrosine 163-171 cyclin dependent kinase 1 Homo sapiens 158-162 12811820-6 2003 Caffeine induced G(2)/M phase cell cycle arrest in NB4 cells in association with the induction of phosphorylation at the Ser-15 residue of p53 and induction of tyrosine phosphorylation of cdc2. Tyrosine 160-168 cyclin dependent kinase 1 Homo sapiens 188-192 12894503-3 2003 Caffeine, a nonspecific inhibitor of ATR, enhanced the cytotoxic effect of cisplatin, modestly decreased the p53 and p21WAF-1 response to cisplatin, and affected the cdc2-p34/cyclin B1 complex by decreasing both cyclin B1 protein accumulation and cdc2-p34 tyrosine 15 phosphorylation. Tyrosine 256-264 cyclin dependent kinase 1 Homo sapiens 166-170 12973835-5 2003 The underlying molecular mechanism is that ErbB2 inhibits p34(Cdc2) activation, which is required for taxol-induced apoptosis, by up-regulating p21(Cip1) and by hyperphosphorylating p34(Cdc2) on tyrosine-15. Tyrosine 195-203 cyclin dependent kinase 1 Homo sapiens 62-66 12915131-6 2003 Aberrant levels of cyclin E and p21(Cip/WAF-1) expression as well as Cdc 2 phosphorylation at Tyrosine 15 suggest that perturbation of G1/S and G2/M transitions in cell cycle might be responsible for 5-FU triggered apoptosis. Tyrosine 94-102 cyclin dependent kinase 1 Homo sapiens 69-74 12669309-5 2003 The inhibitory phosphorylation of CDC2 on Tyr-15 was significantly elevated at 12-24 h, and its levels gradually declined during G2 arrest in Hep3B. Tyrosine 42-45 cyclin dependent kinase 1 Homo sapiens 34-38 12669309-6 2003 In particular, augmentation of CDK inhibitors p21cip1 and p27kip1 and Wee1 kinase and diminution of CDC25C phosphatase coincided with induced Tyr-15 phosphorylation and inhibition of CDC2. Tyrosine 142-145 cyclin dependent kinase 1 Homo sapiens 100-104 12472573-2 2002 Arrest of the cell cycle is mediated by retaining an inactivating state of phosphorylation of cyclin-dependent kinase 1 (Cdk1) on tyrosine 15. Tyrosine 130-138 cyclin dependent kinase 1 Homo sapiens 94-119 12472573-2 2002 Arrest of the cell cycle is mediated by retaining an inactivating state of phosphorylation of cyclin-dependent kinase 1 (Cdk1) on tyrosine 15. Tyrosine 130-138 cyclin dependent kinase 1 Homo sapiens 121-125 12472573-6 2002 Only HL60 cells that subsequently underwent apoptosis elicited the same effect of increased Cdk1 tyrosine phosphorylation. Tyrosine 97-105 cyclin dependent kinase 1 Homo sapiens 92-96 12472573-7 2002 It is proposed that the augmented tyrosine phosphorylation status of Cdk1 is associated with the abolition of cell survival, in addition to the previously reported induction of cell cycle arrest in myeloid cell lines. Tyrosine 34-42 cyclin dependent kinase 1 Homo sapiens 69-73 12384528-5 2002 Herceptin plus Taxol treatment led to higher levels of p34(Cdc2) kinase activity and apoptosis in ErbB2-overexpressing breast cancer cells, which is likely attributable to inhibition of Cdc2-Tyr-15 phosphorylation and p21(Cip1) expression. Tyrosine 191-194 cyclin dependent kinase 1 Homo sapiens 186-190 12384528-4 2002 Herceptin treatment down-regulated ErbB2, reduced the inhibitory phosphorylation of Cdc2 on Tyr-15, and down-regulated the expression of p21(Cip1), a Cdc2 inhibitor. Tyrosine 92-95 cyclin dependent kinase 1 Homo sapiens 84-88 12384528-6 2002 Because significant dephosphorylation of Cdc2-Tyr-15 and down-regulation of p21(Cip1) occur at least 24 h after Herceptin treatment, we investigated whether 24 h Herceptin pretreatment will render ErbB2-overexpressing breast cancer cells more sensitive to Taxol-induced apoptosis compared with the simultaneous treatment of Herceptin plus Taxol. Tyrosine 46-49 cyclin dependent kinase 1 Homo sapiens 41-45 12384528-8 2002 Thus, Herceptin treatment of ErbB2-overexpressing cells can inhibit ErbB2-mediated Cdc2-Tyr-15 phosphorylation and p21(Cip1) up-regulation, which allows effective p34(Cdc2) activation and induction of apoptosis upon Taxol treatment. Tyrosine 88-91 cyclin dependent kinase 1 Homo sapiens 83-87 12222840-0 2002 Suppression of Cdc2 dephosphorylation at the tyrosine 15 residue during nitrosourea-induced G2M phase arrest in glioblastoma cell lines. Tyrosine 45-53 cyclin dependent kinase 1 Homo sapiens 15-19 12222840-6 2002 In both cell lines, the amount of Cdc2 protein phosphorylated at the tyrosine 15 residue was increased 2- to 6-fold by treatment with ACNU compared with untreated control cells. Tyrosine 69-77 cyclin dependent kinase 1 Homo sapiens 34-38 12049736-0 2002 Phosphorylation on tyrosine-15 of p34(Cdc2) by ErbB2 inhibits p34(Cdc2) activation and is involved in resistance to taxol-induced apoptosis. Tyrosine 19-27 cyclin dependent kinase 1 Homo sapiens 38-42 12049736-0 2002 Phosphorylation on tyrosine-15 of p34(Cdc2) by ErbB2 inhibits p34(Cdc2) activation and is involved in resistance to taxol-induced apoptosis. Tyrosine 19-27 cyclin dependent kinase 1 Homo sapiens 66-70 12049736-3 2002 Here, we report that the inhibitory phosphorylation on Cdc2 tyrosine (Y)15 (Cdc2-Y15-p) is elevated in ErbB2-overexpressing breast cancer cells and primary tumors. Tyrosine 60-68 cyclin dependent kinase 1 Homo sapiens 55-59 12049736-3 2002 Here, we report that the inhibitory phosphorylation on Cdc2 tyrosine (Y)15 (Cdc2-Y15-p) is elevated in ErbB2-overexpressing breast cancer cells and primary tumors. Tyrosine 60-68 cyclin dependent kinase 1 Homo sapiens 76-80 11952644-6 2002 The extent of tyrosine 15-phosphorylated p34CDC2 was assessed in the nuclear fractions. Tyrosine 14-22 cyclin dependent kinase 1 Homo sapiens 41-48 11896603-1 2002 Cdc25 activates maturation promoting factor (MPF) and promotes mitosis by removing the inhibitory phosphate from the Tyr-15 of Cdc2 in human cells. Tyrosine 117-120 cyclin dependent kinase 1 Homo sapiens 0-4 11807090-10 2002 Specifically, the Chfr pathway prolongs the phosphorylated state of tyrosine 15 in Cdc2. Tyrosine 68-76 cyclin dependent kinase 1 Homo sapiens 83-87 11839095-1 2002 The WEE1 protein kinase carries out the inhibitory phosphorylation of CDC2 on tyrosine 15 (Tyr15), which is required for activation of the G(2)-phase checkpoint in response to DNA damage. Tyrosine 78-86 cyclin dependent kinase 1 Homo sapiens 70-74 11841447-7 2002 We found that the expression of the cell cycle checkpoint protein Chk1 was reduced in the etoposide-treated p53-transfected cells by 24 h, and this correlated with a reduction in the extent of etoposide-induced phosphorylation of CDK1 at tyrosine 15 (Y15). Tyrosine 238-246 cyclin dependent kinase 1 Homo sapiens 230-234 12658801-8 2002 Upon irradiation, p34cdc2 in U937-pZeosv2(+) was maintained in an inactive state by phosphorylation on threonine 14 (Thr 14) and tyrosine 15 (Tyr 15), which was associated with a dramatic decrease of nuclear CDC25A, CDC25B and CDC25C proteins. Tyrosine 129-137 cyclin dependent kinase 1 Homo sapiens 18-25 11782384-0 2002 Abolishment of the Tyr-15 inhibitory phosphorylation site on cdc2 reduces the radiation-induced G(2) delay, revealing a potential checkpoint in early mitosis. Tyrosine 19-22 cyclin dependent kinase 1 Homo sapiens 61-65 11782384-1 2002 cdc2 is inactivated before mitosis by phosphorylation at its inhibitory sites, Thr-14 and Tyr-15. Tyrosine 90-93 cyclin dependent kinase 1 Homo sapiens 0-4 11782384-4 2002 We have shown that the radiation-induced G(2) delay is preserved in wild-type or cdc2-AY-transfected cells (which retain Tyr-15); this delay is abolished in cdc2-TF- or cdc2-AF-transfected cells (which lack Tyr-15). Tyrosine 121-124 cyclin dependent kinase 1 Homo sapiens 81-85 11782384-4 2002 We have shown that the radiation-induced G(2) delay is preserved in wild-type or cdc2-AY-transfected cells (which retain Tyr-15); this delay is abolished in cdc2-TF- or cdc2-AF-transfected cells (which lack Tyr-15). Tyrosine 207-210 cyclin dependent kinase 1 Homo sapiens 157-161 11782384-4 2002 We have shown that the radiation-induced G(2) delay is preserved in wild-type or cdc2-AY-transfected cells (which retain Tyr-15); this delay is abolished in cdc2-TF- or cdc2-AF-transfected cells (which lack Tyr-15). Tyrosine 207-210 cyclin dependent kinase 1 Homo sapiens 157-161 12658801-8 2002 Upon irradiation, p34cdc2 in U937-pZeosv2(+) was maintained in an inactive state by phosphorylation on threonine 14 (Thr 14) and tyrosine 15 (Tyr 15), which was associated with a dramatic decrease of nuclear CDC25A, CDC25B and CDC25C proteins. Tyrosine 142-145 cyclin dependent kinase 1 Homo sapiens 18-25 12658801-9 2002 In contrast, p34cdc2 in U937-ASPI3K maintained in an active state by dephosphorylation on threonine 14 (Thr 14) and tyrosine 15 (Tyr 15), which was associated with constant nuclear CDC25A, CDC25B and CDC25C protein abundance before and after irradiation. Tyrosine 116-124 cyclin dependent kinase 1 Homo sapiens 13-20 12658801-9 2002 In contrast, p34cdc2 in U937-ASPI3K maintained in an active state by dephosphorylation on threonine 14 (Thr 14) and tyrosine 15 (Tyr 15), which was associated with constant nuclear CDC25A, CDC25B and CDC25C protein abundance before and after irradiation. Tyrosine 129-132 cyclin dependent kinase 1 Homo sapiens 13-20 11719462-7 2001 In an attempt to define the signal transduction mechanisms responsible for MMR-mediated G(2) arrest, we examined the levels of tyrosine 15 phosphorylation of cdc2 (phospho-Tyr15-cdc2), a key regulator of the G(2)-M transition. Tyrosine 127-135 cyclin dependent kinase 1 Homo sapiens 158-162 11751453-1 2001 The mitotic inducer Cdc2 is negatively regulated, in part, by phosphorylation on tyrosine 15. Tyrosine 81-89 cyclin dependent kinase 1 Homo sapiens 20-24 11751453-2 2001 Human Wee1 is a tyrosine-specific protein kinase that phosphorylates Cdc2 on tyrosine 15. Tyrosine 16-24 cyclin dependent kinase 1 Homo sapiens 69-73 11719452-6 2001 At the cellular level, 0.5 microM PD0166285 dramatically inhibits irradiation-induced Cdc2 phosphorylation at the Tyr-15 and Thr-14 in seven of seven cancer cell lines tested. Tyrosine 114-117 cyclin dependent kinase 1 Homo sapiens 86-90 11719462-7 2001 In an attempt to define the signal transduction mechanisms responsible for MMR-mediated G(2) arrest, we examined the levels of tyrosine 15 phosphorylation of cdc2 (phospho-Tyr15-cdc2), a key regulator of the G(2)-M transition. Tyrosine 127-135 cyclin dependent kinase 1 Homo sapiens 178-182 11598051-10 2001 Whereas cyclin B1 was not detectably affected in E22-exposed cells, Cdk1 was maintained in a tyrosine-phosphorylated inactive state and lost its affinity for p13(suc1)-agarose beads. Tyrosine 93-101 cyclin dependent kinase 1 Homo sapiens 68-72 11500387-4 2001 Under prolonged expression of the c-fos gene, however, the amount of wee1 kinase was increased and its target cdc2 molecule was constitutively phosphorylated on its tyrosine residue, where Th1 cells went into aberrant mitosis. Tyrosine 165-173 cyclin dependent kinase 1 Homo sapiens 110-114 10854050-5 2000 We provide evidence that a portion of the cdc2/cyclin B1 translocated into the nucleus in prophase is the inactive tyrosine-15-phosphorylated form. Tyrosine 115-123 cyclin dependent kinase 1 Homo sapiens 42-46 11306507-8 2001 Moreover, induction of tyrosine phosphorylation of CDC2 and reduction of CDC2 kinase activity after irradiation was less significant in TE8-CDC25B than in TE8-neo. Tyrosine 23-31 cyclin dependent kinase 1 Homo sapiens 51-55 11098059-6 2001 Coinciding with the decrease in cyclin B1-Cdc2 activity, there is an increase in tyrosine phosphorylation of Cdc2, suggesting that an increase in the inactive Tyr-15-phosphorylated form of Cdc2 is involved in the decrease in cyclin B1-Cdc2 activity and G(2) arrest. Tyrosine 81-89 cyclin dependent kinase 1 Homo sapiens 109-113 11098059-6 2001 Coinciding with the decrease in cyclin B1-Cdc2 activity, there is an increase in tyrosine phosphorylation of Cdc2, suggesting that an increase in the inactive Tyr-15-phosphorylated form of Cdc2 is involved in the decrease in cyclin B1-Cdc2 activity and G(2) arrest. Tyrosine 81-89 cyclin dependent kinase 1 Homo sapiens 109-113 11098059-6 2001 Coinciding with the decrease in cyclin B1-Cdc2 activity, there is an increase in tyrosine phosphorylation of Cdc2, suggesting that an increase in the inactive Tyr-15-phosphorylated form of Cdc2 is involved in the decrease in cyclin B1-Cdc2 activity and G(2) arrest. Tyrosine 81-89 cyclin dependent kinase 1 Homo sapiens 109-113 11098059-6 2001 Coinciding with the decrease in cyclin B1-Cdc2 activity, there is an increase in tyrosine phosphorylation of Cdc2, suggesting that an increase in the inactive Tyr-15-phosphorylated form of Cdc2 is involved in the decrease in cyclin B1-Cdc2 activity and G(2) arrest. Tyrosine 159-162 cyclin dependent kinase 1 Homo sapiens 109-113 11098059-6 2001 Coinciding with the decrease in cyclin B1-Cdc2 activity, there is an increase in tyrosine phosphorylation of Cdc2, suggesting that an increase in the inactive Tyr-15-phosphorylated form of Cdc2 is involved in the decrease in cyclin B1-Cdc2 activity and G(2) arrest. Tyrosine 159-162 cyclin dependent kinase 1 Homo sapiens 109-113 11098059-6 2001 Coinciding with the decrease in cyclin B1-Cdc2 activity, there is an increase in tyrosine phosphorylation of Cdc2, suggesting that an increase in the inactive Tyr-15-phosphorylated form of Cdc2 is involved in the decrease in cyclin B1-Cdc2 activity and G(2) arrest. Tyrosine 159-162 cyclin dependent kinase 1 Homo sapiens 109-113 10770932-5 2000 We show that bFGF treatment inhibits Tyr-15 dephosphorylation of cdc2 and prevents activation of Cdc25C, similar to what is seen upon activation of the G(2) DNA damage checkpoint. Tyrosine 37-40 cyclin dependent kinase 1 Homo sapiens 65-69 11283255-8 2001 Several observations suggested that the latter cells were in G2 phase, prior to the onset of mitosis: these cells contained uncondensed chromosomes, low levels of cyclin B-associated kinase activity, and high levels of tyrosine-phosphorylated Cdk1. Tyrosine 219-227 cyclin dependent kinase 1 Homo sapiens 243-247 11163155-2 2001 In some eukaryotic cells, mitotic entry relies heavily on regulation of the state of tyrosine phosphorylation of the cyclin-dependent kinase Cdc2. Tyrosine 85-93 cyclin dependent kinase 1 Homo sapiens 141-145 11163155-3 2001 Evidence that checkpoint regulation of cell-cycle progression operates through controlling the state of Cdc2 tyrosine phosphorylation exists. Tyrosine 109-117 cyclin dependent kinase 1 Homo sapiens 104-108 11106571-7 2000 After immunoprecipitation from control and AD tissue, we found that the tyrosine dephosphorylating activity of Cdc25A against exogenous Cdc2 substrate was elevated in AD. Tyrosine 72-80 cyclin dependent kinase 1 Homo sapiens 111-115 11027648-5 2000 However, we observed an accumulation of tyrosine 15 hyperphosphorylated cyclin B-cdc2 complex. Tyrosine 40-48 cyclin dependent kinase 1 Homo sapiens 81-85 11027648-7 2000 Chk1 phosphorylates cdc25C on serine 216 and inactivates it whereas cdc25C dephosphorylates tyrosine 15 phosphate of cdc2 and activates the cdc2-cyclin B complex. Tyrosine 92-100 cyclin dependent kinase 1 Homo sapiens 68-72 11029659-1 2000 BACKGROUND: In eukaryotic cells, the kinase activity of the mitosis-promoting complex composed of cyclin B and Cdc2 (Cdk1) is negatively regulated by the phosphorylation of Cdk1 on threonine or tyrosine residues within its ATP binding domain. Tyrosine 194-202 cyclin dependent kinase 1 Homo sapiens 111-115 11029659-1 2000 BACKGROUND: In eukaryotic cells, the kinase activity of the mitosis-promoting complex composed of cyclin B and Cdc2 (Cdk1) is negatively regulated by the phosphorylation of Cdk1 on threonine or tyrosine residues within its ATP binding domain. Tyrosine 194-202 cyclin dependent kinase 1 Homo sapiens 117-121 11029659-1 2000 BACKGROUND: In eukaryotic cells, the kinase activity of the mitosis-promoting complex composed of cyclin B and Cdc2 (Cdk1) is negatively regulated by the phosphorylation of Cdk1 on threonine or tyrosine residues within its ATP binding domain. Tyrosine 194-202 cyclin dependent kinase 1 Homo sapiens 173-177 11029659-8 2000 Recombinant Wee1B effectively phosphorylated cyclin B-associated Cdk1 on tyrosine-15, resulting in an inactivation of the kinase activity of Cdk1. Tyrosine 73-81 cyclin dependent kinase 1 Homo sapiens 65-69 11029659-8 2000 Recombinant Wee1B effectively phosphorylated cyclin B-associated Cdk1 on tyrosine-15, resulting in an inactivation of the kinase activity of Cdk1. Tyrosine 73-81 cyclin dependent kinase 1 Homo sapiens 141-145 10886374-1 2000 BACKGROUND: Activation of the cyclin-dependent kinase cdc2-cyclin B1 at the G2/M transition of the cell cycle requires dephosphorylation of threonine-14 and tyrosine-15 in cdc2, which in higher eukaryotes is brought about by the Cdc25C phosphatase. Tyrosine 157-165 cyclin dependent kinase 1 Homo sapiens 54-58 10886374-1 2000 BACKGROUND: Activation of the cyclin-dependent kinase cdc2-cyclin B1 at the G2/M transition of the cell cycle requires dephosphorylation of threonine-14 and tyrosine-15 in cdc2, which in higher eukaryotes is brought about by the Cdc25C phosphatase. Tyrosine 157-165 cyclin dependent kinase 1 Homo sapiens 172-176 10809772-3 2000 Here we show that genistein exerts this effect by impairing the Cdc25C-dependent Tyr-15 dephosphorylation of Cdk1, as the overexpression of this phosphatase allows the cells to escape G(2) arrest and enter an abnormal chromatin condensation stage. Tyrosine 81-84 cyclin dependent kinase 1 Homo sapiens 109-113 10496881-6 1999 For its activation at the G2/M border, p34cdc2 needs to be associated with cyclin B and additionally dephosphorylated at Tyr-15 by the specific phosphatase cdc25-C. Tyrosine 121-124 cyclin dependent kinase 1 Homo sapiens 39-46 10564539-6 1999 Despite this decrease in activity, the inhibitory tyrosine phosphorylation of p34cdc2 was decreased, suggesting that other factors are responsible for the decreased kinase activity. Tyrosine 50-58 cyclin dependent kinase 1 Homo sapiens 78-85 10574948-6 1999 Irradiation led to nuclear accumulation of the inactive tyrosine-phosphorylated form of p34(Cdc2), whereas the active form was seen in the cytoplasm. Tyrosine 56-64 cyclin dependent kinase 1 Homo sapiens 92-96 10496881-7 1999 Treatment of synchronized cells in S or G2 phase with C. botulinum C2 toxin prevented p34cdc2 protein kinase activation by inhibiting its tyrosine dephosphorylation at the G2/M border. Tyrosine 138-146 cyclin dependent kinase 1 Homo sapiens 86-93 10368682-3 1999 Here we report that upon Etoposide treatment CDC2 is phosphorylated on tyrosine 15 and is dephosphorylated and activated in vitro by recombinant CDC25 phosphatase. Tyrosine 71-79 cyclin dependent kinase 1 Homo sapiens 45-49 10373560-1 1999 The Myt1 protein kinase functions to negatively regulate Cdc2-cyclin B complexes by phosphorylating Cdc2 on threonine 14 and tyrosine 15. Tyrosine 125-133 cyclin dependent kinase 1 Homo sapiens 57-61 10360785-4 1999 The results from these experiments demonstrate that concomitant exposure of HL60 cells to caffeine (2 mM) during irradiation inhibited radiation-induced tyrosine 15 phosphorylation of the G2/M-phase transition checkpoint protein CDC2/p34 kinase and reduced G2/M-phase arrest by 40-46% compared to cells irradiated without caffeine. Tyrosine 153-161 cyclin dependent kinase 1 Homo sapiens 229-233 10037143-7 1999 Furthermore, we found that after PMA treatment p21 was able to associate with the active Tyr-15 dephosphorylated form of cdc2, but this complex was devoid of kinase activity indicating that p21 may play a role in inhibition of cdc2 induced by PMA. Tyrosine 89-92 cyclin dependent kinase 1 Homo sapiens 121-125 10098730-0 1999 Butyrate augments interferon-alpha-induced S phase accumulation and persistent tyrosine phosphorylation of cdc2 in K562 cells. Tyrosine 79-87 cyclin dependent kinase 1 Homo sapiens 107-111 10098730-6 1999 The ability of leukaemic cells to exhibit the S phase accumulation after stimulation by IFN-alpha plus sodium butyrate correlated well with persistent tyrosine phosphorylation of cdc2, whereas treatment with IFN-gamma plus sodium butyrate did not affect its phosphorylation levels. Tyrosine 151-159 cyclin dependent kinase 1 Homo sapiens 179-183 10098730-7 1999 Considering that dephosphorylation of cdc2 leads to entry to the M phase, the persistent tyrosine phosphorylation of cdc2 may be associated with the S phase accumulation induced by IFN-alpha and sodium butyrate. Tyrosine 89-97 cyclin dependent kinase 1 Homo sapiens 38-42 10098730-7 1999 Considering that dephosphorylation of cdc2 leads to entry to the M phase, the persistent tyrosine phosphorylation of cdc2 may be associated with the S phase accumulation induced by IFN-alpha and sodium butyrate. Tyrosine 89-97 cyclin dependent kinase 1 Homo sapiens 117-121 9844922-6 1998 In addition, the in vitro histone H1 kinase activity of immunoprecipitated CDK1 and its tyrosine phosphorylation status (by anti-phosphotyrosine immunoblot analysis) were determined. Tyrosine 88-96 cyclin dependent kinase 1 Homo sapiens 75-79 10194549-7 1999 In addition, augmentation of CDC2 protein tyrosine phosphorylation was induced only when the cells were treated with STP. Tyrosine 42-50 cyclin dependent kinase 1 Homo sapiens 29-33 9543386-1 1998 Cdc25C is a dual-specificity protein kinase that controls entry into mitosis by dephosphorylating Cdc2 on both threonine 14 and tyrosine 15. Tyrosine 128-136 cyclin dependent kinase 1 Homo sapiens 0-4 9770514-5 1998 The majority of cdc2, however, was tyrosine-phosphorylated and therefore enzymatically inactive. Tyrosine 35-43 cyclin dependent kinase 1 Homo sapiens 16-20 9683525-18 1998 Thus, the arrest of growth in G2 appears to be due to the inhibition of the p34(cdc2) kinase activity which is associated with the increased expression of p21(Cip1/Waf1) and increased phosphorylation on tyrosine of p34(cdc2). Tyrosine 203-211 cyclin dependent kinase 1 Homo sapiens 80-84 9578617-5 1998 Cyclin B-, but not cyclin A-, associated Cdc2 was subjected to regulation via Tyr phosphorylation, and phosphorylation states of Cdc25 correlated with cyclin B/Cdc2 kinase activity with some exceptions. Tyrosine 78-81 cyclin dependent kinase 1 Homo sapiens 41-45 9820491-7 1998 The decrease in cdk1 activity observed in T lymphocytes from aged donors could be accounted for by at least three mechanisms: 1) a failure of these cells to express a sufficient amount of cdk1, 2) a reduced level of the associated cyclin B1, and 3) an incomplete dephosphorylation of the kinase on tyrosine. Tyrosine 298-306 cyclin dependent kinase 1 Homo sapiens 16-20 9573072-4 1998 CDT treatment was also shown to cause HeLa cells to accumulate the inactive, tyrosine-phosphorylated form of CDC2. Tyrosine 77-85 cyclin dependent kinase 1 Homo sapiens 109-113 9492343-3 1998 Progression from G2 into M is controlled in part by a cyclin-dependent kinase (cyclin B/Cdk1) that is regulated by tyrosine phosphorylation. Tyrosine 115-123 cyclin dependent kinase 1 Homo sapiens 88-92 9438385-8 1998 TPA inhibited Taxol-mediated activation of p34cdc2 kinase by preventing the dephosphorylation of the Tyr-15 residue on p34cdc2 without altering the levels of Cdc2 and cyclin B1. Tyrosine 101-104 cyclin dependent kinase 1 Homo sapiens 43-50 9438385-8 1998 TPA inhibited Taxol-mediated activation of p34cdc2 kinase by preventing the dephosphorylation of the Tyr-15 residue on p34cdc2 without altering the levels of Cdc2 and cyclin B1. Tyrosine 101-104 cyclin dependent kinase 1 Homo sapiens 119-126 9268380-2 1997 This step is mediated principally by the dephosphorylation of residues threonine 14 (Thr14) and tyrosine 15 (Tyr15) on the Cdc2 catalytic subunit. Tyrosine 96-104 cyclin dependent kinase 1 Homo sapiens 123-127 9407133-0 1997 Proteolysis and tyrosine phosphorylation of p34cdc2/cyclin B. Tyrosine 16-24 cyclin dependent kinase 1 Homo sapiens 44-51 9407133-1 1997 The role of MCM2 and initiation of DNA replication to allow tyrosine phosphorylation of p34cdc2. Tyrosine 60-68 cyclin dependent kinase 1 Homo sapiens 88-95 9407133-5 1997 Cells arrested at G1/S by lack of nimQMCM2 contain p34(cdc2)/cyclin B, but p34(cdc2) remains tyrosine dephosphorylated, even after DNA damage. Tyrosine 93-101 cyclin dependent kinase 1 Homo sapiens 79-83 9407133-7 1997 nimQMCM2, likely via initiation of DNA replication, is therefore required to trigger tyrosine phosphorylation of p34(cdc2) during the G1 to S transition, which may occur by inactivation of nimTcdc25. Tyrosine 85-93 cyclin dependent kinase 1 Homo sapiens 117-121 9407133-9 1997 Initiation of DNA replication, which requires nimQMCM2, is apparently critical to switch mitotic regulation from the APC to include tyrosine phosphorylation of p34(cdc2) at G1/S. Tyrosine 132-140 cyclin dependent kinase 1 Homo sapiens 164-168 9288185-0 1997 Transient tyrosine phosphorylation of p34cdc2 is an early event in radiation-induced apoptosis of prostate cancer cells. Tyrosine 10-18 cyclin dependent kinase 1 Homo sapiens 38-45 9288185-6 1997 Subsequent analysis revealed that the substrate for this tyrosine phosphorylation is the serine/ threonine p34cdc2 protein kinase, a cell cycle regulatory protein that controls cell entry into mitosis. Tyrosine 57-65 cyclin dependent kinase 1 Homo sapiens 107-114 9288185-8 1997 Arresting the cells in G0/G1 phase by pretreatment with suramin totally abrogated radiation-induced phosphorylation of p34cdc2 protein at the tyrosine residue, indicating that this posttranslational modification occurs in cell populations that escape G2 arrest and undergo apoptosis in response to radiation. Tyrosine 142-150 cyclin dependent kinase 1 Homo sapiens 119-126 9407044-2 1997 Dephosphorylation of threonine 14 and tyrosine 15 of CDK1 by the CDC25 phosphatases is a key step in the activation of the CDK1-cyclin B protein kinase. Tyrosine 38-46 cyclin dependent kinase 1 Homo sapiens 53-57 9407044-2 1997 Dephosphorylation of threonine 14 and tyrosine 15 of CDK1 by the CDC25 phosphatases is a key step in the activation of the CDK1-cyclin B protein kinase. Tyrosine 38-46 cyclin dependent kinase 1 Homo sapiens 123-127 9377557-0 1997 Sustained accumulation of the mitotic cyclins and tyrosine-phosphorylated p34cdc2 in human G1-S-arrested cancer cells but not untransformed cells. Tyrosine 50-58 cyclin dependent kinase 1 Homo sapiens 74-81 9377557-3 1997 Under such conditions, indeed, cancer cells do continue to accumulate cyclin A, cyclin B1, and tyrosine-phosphorylated p34cdc2 to supranormal levels, a phenomenon that does not occur in untransformed, nonimmortalized human fibroblasts. Tyrosine 95-103 cyclin dependent kinase 1 Homo sapiens 119-126 8944784-4 1996 In the presence of vanadate, the H1 kinase activity dropped to near normal levels and the amount of the inactive from of p34cdc2 protein phosphorylated at a tyrosine residue was increased. Tyrosine 157-165 cyclin dependent kinase 1 Homo sapiens 121-128 17708961-3 1997 The mitotic regulators targeted for negative regulation by these checkpoints differ among cell types, but two conserved targets have emerged, the anaphase-promoting complex (APC) and tyrosine phosphorylation of p34(cdc2). Tyrosine 183-191 cyclin dependent kinase 1 Homo sapiens 215-219 9187128-4 1997 This G2-M arrest was mediated by persistent inactivation of the cyclin B1/cdc2 kinase complex characterized by both sustained accumulation of cyclin B1 and tyrosine phosphorylation of cdc2. Tyrosine 156-164 cyclin dependent kinase 1 Homo sapiens 74-78 9187128-4 1997 This G2-M arrest was mediated by persistent inactivation of the cyclin B1/cdc2 kinase complex characterized by both sustained accumulation of cyclin B1 and tyrosine phosphorylation of cdc2. Tyrosine 156-164 cyclin dependent kinase 1 Homo sapiens 184-188 9201712-2 1997 In human cells, the mitosis-inducing kinase CDC2/cyclin B is inhibited by phosphorylation of threonine-14 and tyrosine-15, but the roles of these phosphorylations in enforcing checkpoints is not known. Tyrosine 110-118 cyclin dependent kinase 1 Homo sapiens 44-48 9034337-3 1997 Studies in mammalian cells have correlated induction of inhibitory tyrosine 15 (Y15) phosphorylation on p34cdc2 with the response to DNA damage. Tyrosine 67-75 cyclin dependent kinase 1 Homo sapiens 104-111 8670863-2 1996 The first responds to the rate of DNA replication and inhibits mitosis via tyrosine phosphorylation of p34cdc2. Tyrosine 75-83 cyclin dependent kinase 1 Homo sapiens 103-110 8910383-0 1996 Sequential dephosphorylation of p34(cdc2) on Thr-14 and Tyr-15 at the prophase/metaphase transition. Tyrosine 56-59 cyclin dependent kinase 1 Homo sapiens 36-40 8910383-2 1996 During the prophase/metaphase transition, the inactive, Thr-14/Tyr-15 phosphorylated form of p34(cdc2) (TP-YP) is modified to an active, Thr-14/Tyr-15 dephosphorylated form (T-Y) by the cdc25 dual-specificity phosphatase. Tyrosine 63-66 cyclin dependent kinase 1 Homo sapiens 97-101 8816913-2 1996 The kinase activity of active MPF is not inhibited; rather, the conversion of pre-MPF to MPF, i.e., the activating dephosphorylation of p34cdc2, at tyrosine is rapidly blocked (Barth and Kinzel, 1994, Exp. Tyrosine 148-156 cyclin dependent kinase 1 Homo sapiens 136-143 8670863-3 1996 Cells unable to tyrosine phosphorylate p34cdc2 are therefore viable but are unable to tolerate low levels of hydroxyurea and prematurely enter lethal mitosis when S-phase is slowed. Tyrosine 16-24 cyclin dependent kinase 1 Homo sapiens 39-46 8670863-4 1996 However, if the NIMA mitosis-promoting kinase is inactivated then non-tyrosine-phosphorylated p34cdc2 cannot promote cells prematurely into mitosis. Tyrosine 70-78 cyclin dependent kinase 1 Homo sapiens 94-101 8670863-5 1996 Lack of tyrosine-phosphorylated p34cdc2 also cannot promote mitosis, or lethality, if DNA replication is arrested, demonstrating the presence of a second S-phase checkpoint mechanism over mitotic initiation which we show involves the function of BIME. Tyrosine 8-16 cyclin dependent kinase 1 Homo sapiens 32-39 8670863-6 1996 In order to overcome the S-phase arrest checkpoint over mitosis it is necessary both to prevent tyrosine phosphorylation of p34cdc2 and also to inactivate BIME. Tyrosine 96-104 cyclin dependent kinase 1 Homo sapiens 124-131 8670863-7 1996 Lack of tyrosine phosphorylation of p34cdc2 allows precocious expression of NIMA during S-phase arrest, and lack of BIME then allows activation of this prematurely expressed NIMA by phosphorylation. Tyrosine 8-16 cyclin dependent kinase 1 Homo sapiens 36-43 8662825-8 1996 Taken together, these results suggest that different CDKs are inhibited by different mechanisms following UV-induced DNA damage: Cdk2 is inhibited by the elevated level of p21; Cdk4 is inhibited by cooperation of p21 with other CDK inhibitors, like p27, and possibly by phosphorylation; and Cdc2 is inhibited by Thr-14/Tyr-15 phosphorylation. Tyrosine 319-322 cyclin dependent kinase 1 Homo sapiens 53-56 8662825-7 1996 Cdc2 is likely to be inhibited by Thr-14/Tyr-15 phosphorylation after irradiation because Cdc2 was tyrosine-phosphorylated, and recombinant Cdc25 was able to increase its kinase activity significantly. Tyrosine 41-44 cyclin dependent kinase 1 Homo sapiens 0-4 8626437-1 1996 Exposure of human B-cell precursors (BCP) to ionizing radiation results in cell cycle arrest at the G2-M checkpoint as a result of inhibitory tyrosine phosphorylation of p34cdc2 . Tyrosine 142-150 cyclin dependent kinase 1 Homo sapiens 170-177 8662825-7 1996 Cdc2 is likely to be inhibited by Thr-14/Tyr-15 phosphorylation after irradiation because Cdc2 was tyrosine-phosphorylated, and recombinant Cdc25 was able to increase its kinase activity significantly. Tyrosine 41-44 cyclin dependent kinase 1 Homo sapiens 90-94 8662825-7 1996 Cdc2 is likely to be inhibited by Thr-14/Tyr-15 phosphorylation after irradiation because Cdc2 was tyrosine-phosphorylated, and recombinant Cdc25 was able to increase its kinase activity significantly. Tyrosine 99-107 cyclin dependent kinase 1 Homo sapiens 0-4 8662825-7 1996 Cdc2 is likely to be inhibited by Thr-14/Tyr-15 phosphorylation after irradiation because Cdc2 was tyrosine-phosphorylated, and recombinant Cdc25 was able to increase its kinase activity significantly. Tyrosine 99-107 cyclin dependent kinase 1 Homo sapiens 90-94 8662825-8 1996 Taken together, these results suggest that different CDKs are inhibited by different mechanisms following UV-induced DNA damage: Cdk2 is inhibited by the elevated level of p21; Cdk4 is inhibited by cooperation of p21 with other CDK inhibitors, like p27, and possibly by phosphorylation; and Cdc2 is inhibited by Thr-14/Tyr-15 phosphorylation. Tyrosine 319-322 cyclin dependent kinase 1 Homo sapiens 53-57 8622943-2 1996 cdc2(6-20)NH2 corresponds to the tyrosine phosphorylation site of p34cdc2 kinase, which is phosphorylated by lck kinase (lymphocyte-specific tyrosine kinase, p56lck). Tyrosine 33-41 cyclin dependent kinase 1 Homo sapiens 0-4 8626437-2 1996 Here, we show that ionizing radiation promotes physical interactions between p34cdc2 and the Src family protein-tyrosine kinase Lyn in the cytoplasm of human BCP leading to tyrosine phosphorylation of p34cdc2. Tyrosine 112-120 cyclin dependent kinase 1 Homo sapiens 77-84 8626437-2 1996 Here, we show that ionizing radiation promotes physical interactions between p34cdc2 and the Src family protein-tyrosine kinase Lyn in the cytoplasm of human BCP leading to tyrosine phosphorylation of p34cdc2. Tyrosine 112-120 cyclin dependent kinase 1 Homo sapiens 201-208 8626437-4 1996 Furthermore, Lyn kinase physically associated with and tyrosine-phosphorylated p34cdc2 kinase in vivo when co-expressed in COS-7 cells. Tyrosine 55-63 cyclin dependent kinase 1 Homo sapiens 79-86 8833397-6 1996 Thus the signaling pathway leading to inhibitory tyrosine phosphorylation of p34cdc2 and G2-arrest in irradiated human leukemic B-cell precursors functions independent of p49 WEE1 HU and enzymes which augment the tyrosine kinase activity of p49 WEE 1HU. Tyrosine 49-57 cyclin dependent kinase 1 Homo sapiens 77-84 8822199-6 1996 The tyrosine phosphorylation levels of FAK125, erk1 (mitogen-activated kinase kinase), and cdc-2 were reduced during apoptosis. Tyrosine 4-12 cyclin dependent kinase 1 Homo sapiens 91-96 8822199-7 1996 FAK125 dephosphorylation was inhibited by orthovanadate, but premature activation (tyrosine dephosphorylation) of cdc-2 was not. Tyrosine 83-91 cyclin dependent kinase 1 Homo sapiens 114-119 8833397-1 1996 Here we provide experimental evidence that ionizing radiation induces inhibitory tyrosine phosphorylation of the p34cdc2 kinase in human leukemic B-cell precursors. Tyrosine 81-89 cyclin dependent kinase 1 Homo sapiens 113-120 8833397-2 1996 Herbimycin A markedly reduced tyrosine phosphorylation of p34cdc2 in irradiated leukemic B-cell precursors, thereby preventing radiation-induced cell cycle arrest at the G2-M transition checkpoint. Tyrosine 30-38 cyclin dependent kinase 1 Homo sapiens 58-65 8833397-3 1996 Thus, tyrosine phosphorylation is directly responsible for the inactivation of p34cdc2 in irradiated human leukemic B-cell precursors and activation of protein tyrosine kinases is a proximal and mandatory step in radiation-induced G2-arrest arrest at the G2-M checkpoint. Tyrosine 6-14 cyclin dependent kinase 1 Homo sapiens 79-86 7528141-3 1994 Comparison of the phosphorylation kinetics with those found for the tyrosine residues on a peptide comprising the regulatory tyrosine phosphorylation site of cdc2 points to the role of the primary sequence context of the phosphate acceptor. Tyrosine 68-76 cyclin dependent kinase 1 Homo sapiens 158-162 8772442-5 1996 Although cdc2 was expressed in the presence of PMA, dephosphorylation of its tyrosine residue was inhibited by PMA. Tyrosine 77-85 cyclin dependent kinase 1 Homo sapiens 9-13 8524869-5 1995 Cells rapidly accumulate cyclin B protein and tyrosine-hyperphosphorylated p34cdc2 (cdk1) kinase, indicative of cell cycle arrest at G2 phase. Tyrosine 46-54 cyclin dependent kinase 1 Homo sapiens 75-82 8524869-5 1995 Cells rapidly accumulate cyclin B protein and tyrosine-hyperphosphorylated p34cdc2 (cdk1) kinase, indicative of cell cycle arrest at G2 phase. Tyrosine 46-54 cyclin dependent kinase 1 Homo sapiens 84-88 7478536-4 1995 This inhibition coincided with increased tyrosine phosphorylation of cdc2 and was reversed by the cdc25C phosphatase in vitro. Tyrosine 41-49 cyclin dependent kinase 1 Homo sapiens 69-73 7478536-5 1995 The data indicate that UVB-induced G2 arrest in mammalian cells is mediated by inhibitory tyrosine phosphorylation of cdc2 and acts as a defense mechanism against DNA damage irrespective of the cells" p53 status. Tyrosine 90-98 cyclin dependent kinase 1 Homo sapiens 118-122 7568188-2 1995 Biochemical studies carried out in vitro with recombinant protein demonstrated that WEE1Hu encodes a tyrosine kinase of approximately 49 kDa that phosphorylates p34cdc2 on Tyr-15 [Parker, L. L. & Piwnica-Worms, H. (1992) Science 257, 1955-1957]. Tyrosine 172-175 cyclin dependent kinase 1 Homo sapiens 161-168 7568188-6 1995 Immunoprecipitates of p95 phosphorylated p34cdc2 on Tyr-15, indicating that p95 is functionally related to p49WEEIHu, and mapping studies demonstrated that p95 is structurally related to p49WEE1Hu. Tyrosine 52-55 cyclin dependent kinase 1 Homo sapiens 41-48 7557404-5 1995 The three kinases have conserved Tyr and Thr residues that are sites of phosphorylation in cdc2 and are important for regulating kinase activity. Tyrosine 33-36 cyclin dependent kinase 1 Homo sapiens 91-95 7774574-1 1995 WEE1 kinase negatively regulates entry into mitosis by catalyzing the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase. Tyrosine 81-89 cyclin dependent kinase 1 Homo sapiens 109-113 7712486-3 1995 This G2 delay was strong by 1 h following exposure to IR, maximal by 2 h, and was accompanied by an accumulation of tyrosine-phosphorylated p34CDC2 molecules. Tyrosine 116-124 cyclin dependent kinase 1 Homo sapiens 140-147 7530044-8 1995 Moreover, we demonstrate that (1) p56/p53lyn phosphorylates Tyr-15 of p34cdc2 in vitro and (2) phosphorylation of p34cdc2 by p56/p53lyn inhibits p34cdc2 activity. Tyrosine 60-63 cyclin dependent kinase 1 Homo sapiens 70-77 7530044-8 1995 Moreover, we demonstrate that (1) p56/p53lyn phosphorylates Tyr-15 of p34cdc2 in vitro and (2) phosphorylation of p34cdc2 by p56/p53lyn inhibits p34cdc2 activity. Tyrosine 60-63 cyclin dependent kinase 1 Homo sapiens 114-121 7530044-8 1995 Moreover, we demonstrate that (1) p56/p53lyn phosphorylates Tyr-15 of p34cdc2 in vitro and (2) phosphorylation of p34cdc2 by p56/p53lyn inhibits p34cdc2 activity. Tyrosine 60-63 cyclin dependent kinase 1 Homo sapiens 114-121 7812949-3 1995 This increase is followed by a rapid inactivation of the cyclin B1/Cdc2 kinase that is associated with Cdc2 tyrosine phosphorylation without any change in Cdc2 or cyclin B1 protein levels. Tyrosine 108-116 cyclin dependent kinase 1 Homo sapiens 67-71 7812949-3 1995 This increase is followed by a rapid inactivation of the cyclin B1/Cdc2 kinase that is associated with Cdc2 tyrosine phosphorylation without any change in Cdc2 or cyclin B1 protein levels. Tyrosine 108-116 cyclin dependent kinase 1 Homo sapiens 103-107 7812949-3 1995 This increase is followed by a rapid inactivation of the cyclin B1/Cdc2 kinase that is associated with Cdc2 tyrosine phosphorylation without any change in Cdc2 or cyclin B1 protein levels. Tyrosine 108-116 cyclin dependent kinase 1 Homo sapiens 103-107 7814450-5 1995 The use of antiphosphotyrosine antibodies, however, showed that p34cdc2 from cultures treated with EGF was more intensely stained than that of control cells, indicating that EGF treatment prevents the tyrosine dephosphorylation which is required for expression of the protein kinase activity of the complex. Tyrosine 22-30 cyclin dependent kinase 1 Homo sapiens 64-71 7499827-5 1995 Tyrosine phosphorylation of the 34-kDa cdc2 protein kinase was induced transiently after stimulation of the leukemic T cell line, Molt-3, or peripheral blood T cells. Tyrosine 0-8 cyclin dependent kinase 1 Homo sapiens 39-43 7499827-7 1995 cdc2 kinase activity was constitutive in peripheral blood T cells, and transient inhibition of kinase activity after ICAM-1 stimulation correlated kinetically with phosphorylation of cdc2 on tyrosine. Tyrosine 191-199 cyclin dependent kinase 1 Homo sapiens 0-4 7499827-7 1995 cdc2 kinase activity was constitutive in peripheral blood T cells, and transient inhibition of kinase activity after ICAM-1 stimulation correlated kinetically with phosphorylation of cdc2 on tyrosine. Tyrosine 191-199 cyclin dependent kinase 1 Homo sapiens 183-187 7474080-5 1995 In cycling cells, progression from G2 to M phase is driven by activation of the p34cdc2/cyclin B complex, an event caused, in part, by dephosphorylation of two regulatory amino acids of p34cdc2 (Thr-14 and Tyr-15). Tyrosine 206-209 cyclin dependent kinase 1 Homo sapiens 80-87 7474080-5 1995 In cycling cells, progression from G2 to M phase is driven by activation of the p34cdc2/cyclin B complex, an event caused, in part, by dephosphorylation of two regulatory amino acids of p34cdc2 (Thr-14 and Tyr-15). Tyrosine 206-209 cyclin dependent kinase 1 Homo sapiens 186-193 7743995-1 1995 In higher eukaryotes, the cyclin-dependent kinases (CDKs) are negatively regulated by phosphorylation on threonine 14 (T14) and tyrosine 15 (Y15). Tyrosine 128-136 cyclin dependent kinase 1 Homo sapiens 52-56 7718595-0 1995 1-beta-D-arabinofuranosylcytosine activates tyrosine phosphorylation of p34cdc2 and its association with the Src-like p56/p53lyn kinase in human myeloid leukemia cells. Tyrosine 44-52 cyclin dependent kinase 1 Homo sapiens 72-79 7530044-0 1995 1-beta-D-arabinofuranosylcytosine activates tyrosine phosphorylation of p34cdc2 and its association with the Src-like p56/p53lyn kinase in human myeloid leukemia cells. Tyrosine 44-52 cyclin dependent kinase 1 Homo sapiens 72-79 7530044-3 1995 The results of immunoprecipitation studies demonstrate that HL-60 cells respond to ara-C with tyrosine phosphorylation of the cell cycle regulatory protein p34cdc2 and a decrease in the activity of this kinase. Tyrosine 94-102 cyclin dependent kinase 1 Homo sapiens 156-163 9552365-1 1995 Activation of the cyclin B-cdc2 kinase mitotic inducer involves dephosphorylation of two inhibitory residues, tyrosine 15 and threonine 14, cdc25 is the specific phosphatase that directly dephosphorylates and activates the cdc2 kinase, cdc25 activity is regulated by phosphorylation. Tyrosine 110-118 cyclin dependent kinase 1 Homo sapiens 27-31 9552365-1 1995 Activation of the cyclin B-cdc2 kinase mitotic inducer involves dephosphorylation of two inhibitory residues, tyrosine 15 and threonine 14, cdc25 is the specific phosphatase that directly dephosphorylates and activates the cdc2 kinase, cdc25 activity is regulated by phosphorylation. Tyrosine 110-118 cyclin dependent kinase 1 Homo sapiens 140-144 7528141-3 1994 Comparison of the phosphorylation kinetics with those found for the tyrosine residues on a peptide comprising the regulatory tyrosine phosphorylation site of cdc2 points to the role of the primary sequence context of the phosphate acceptor. Tyrosine 125-133 cyclin dependent kinase 1 Homo sapiens 158-162 7961734-1 1994 Cdc25 protein phosphatase dephosphorylates tyrosine 15 of Cdc2, thereby activating Cdc2/cyclin B kinase, which then brings about mitosis. Tyrosine 43-51 cyclin dependent kinase 1 Homo sapiens 0-4 7961734-1 1994 Cdc25 protein phosphatase dephosphorylates tyrosine 15 of Cdc2, thereby activating Cdc2/cyclin B kinase, which then brings about mitosis. Tyrosine 43-51 cyclin dependent kinase 1 Homo sapiens 58-62 7961734-7 1994 Rescue of cdc25-22 by overproduction of Stp1 protein is probably due to an ability of Stp1 to dephosphorylate tyrosine 15 of Cdc2. Tyrosine 110-118 cyclin dependent kinase 1 Homo sapiens 125-129 8084605-9 1994 We also demonstrate that p34cdc2 undergoes increased phosphorylation on tyrosine following MMC exposure and that p56/p53lyn phosphorylates the Tyr-15 site of p34cdc2 in vitro. Tyrosine 72-80 cyclin dependent kinase 1 Homo sapiens 25-32 7929427-6 1994 Moreover, phosphorylation of Csk-treated pp60c-src by Cdc2 also facilitated complete reactivation by the protein-tyrosine phosphatase CD45 or by a synthetic phosphopeptide corresponding to the C-terminal, regulatory phosphorylation site (Tyr-527). Tyrosine 238-241 cyclin dependent kinase 1 Homo sapiens 54-58 7929427-7 1994 These data indicate that the Src homology 2 domain of Csk-phosphorylated pp60c-src was more accessible for intermolecular interactions and that Tyr-527 was more readily dephosphorylated after treatment with Cdc2. Tyrosine 144-147 cyclin dependent kinase 1 Homo sapiens 207-211 8084605-9 1994 We also demonstrate that p34cdc2 undergoes increased phosphorylation on tyrosine following MMC exposure and that p56/p53lyn phosphorylates the Tyr-15 site of p34cdc2 in vitro. Tyrosine 143-146 cyclin dependent kinase 1 Homo sapiens 25-32 8084605-9 1994 We also demonstrate that p34cdc2 undergoes increased phosphorylation on tyrosine following MMC exposure and that p56/p53lyn phosphorylates the Tyr-15 site of p34cdc2 in vitro. Tyrosine 143-146 cyclin dependent kinase 1 Homo sapiens 158-165 7504471-1 1993 The cdc2-activator cdc25C was immunoprecipitated from HeLa cell extracts and assayed as tyrosine phosphatase (PTP) using tyrosine-phosphorylated myelin basic protein. Tyrosine 88-96 cyclin dependent kinase 1 Homo sapiens 4-8 7945307-3 1994 We also find that the tyrosine phosphorylation of p34cdc2 kinase in the nuclear extract is increased about twice when the extracts are preincubated with 50 microM of suramin prior to the p13-agarose precipitation. Tyrosine 22-30 cyclin dependent kinase 1 Homo sapiens 50-57 8187833-8 1994 However, p34cdc2 from cultures treated with TPA was more intensely stained by anti-phosphotyrosine antibodies than that of control cells, indicating that TPA treatment probably prevented the tyrosine dephosphorylation required for expression of the histone H1 kinase activity of the complex. Tyrosine 90-98 cyclin dependent kinase 1 Homo sapiens 9-16 8076632-7 1994 Similar changes in the behaviour of basic residues were also observed upon tyrosine phosphorylation of a cdc2-derived peptide, suggesting that this potential of phosphorylation events to propagate directed structural changes may find a widespread utility in the activation of protein kinases and in the transduction of phosphorylation-based signalling. Tyrosine 75-83 cyclin dependent kinase 1 Homo sapiens 105-109 8041894-4 1994 The mechanism underlying the G2 arrest may involve suppression of cyclin B1 mRNA and/or protein in some cell lines and tyrosine phosphorylation of p34cdc2 in others. Tyrosine 119-127 cyclin dependent kinase 1 Homo sapiens 147-154 8137239-0 1994 Ionizing radiation induces rapid tyrosine phosphorylation of p34cdc2. Tyrosine 33-41 cyclin dependent kinase 1 Homo sapiens 61-68 8475101-4 1993 The vertebrate CDC2/cyclin B1 complex is an essential regulator of the onset of mitosis and is inhibited by phosphorylation of CDC2 on Thr-14 and Tyr-15. Tyrosine 146-149 cyclin dependent kinase 1 Homo sapiens 15-19 8406990-11 1993 To determine whether the p34cdc2 protein kinase is a primary target for CDDP, we examined the direct effect of CDDP on tyrosine dephosphorylation of p34cdc2 protein kinase in cellular extracts. Tyrosine 119-127 cyclin dependent kinase 1 Homo sapiens 149-156 8402895-1 1993 Cyclin B-cdc2 complexes are kept inactive by inhibitory phosphorylations on Thr-14 and Tyr-15 of cdc2 until they are dephosphorylated at the end of G2 by the phosphatase cdc25. Tyrosine 87-90 cyclin dependent kinase 1 Homo sapiens 9-13 8402895-1 1993 Cyclin B-cdc2 complexes are kept inactive by inhibitory phosphorylations on Thr-14 and Tyr-15 of cdc2 until they are dephosphorylated at the end of G2 by the phosphatase cdc25. Tyrosine 87-90 cyclin dependent kinase 1 Homo sapiens 97-101 8260651-10 1993 In addition, ligation of the IL-2R leads to rapid increases in myc expression and more delayed increases in the expression of the cdc2 and cdk2 kinases and the cyclins through a tyrosine phosphorylation independent pathway. Tyrosine 178-186 cyclin dependent kinase 1 Homo sapiens 130-134 21573322-4 1993 p34cdc2 is a major tyrosine phosphorylated protein in the S- and G2-phases of both HeLa and CHO cells, although fundamental differences appear to exist in p34cdc2 regulation; approximately 80% of this protein is found in its hyperphosphorylated form in CHO cells compared to a maximum of 30% in HeLa cells. Tyrosine 19-27 cyclin dependent kinase 1 Homo sapiens 0-7 21573322-6 1993 Failure to activate the p34cdc2 kinase following etoposide treatment to levels comparable with synchronous G2/M-phase cells is not due to inhibition of p34cdc2/cyclin-B complex formation, but relies more on an inability to tyrosine dephosphorylate p34cdc2. Tyrosine 223-231 cyclin dependent kinase 1 Homo sapiens 24-31 8441405-12 1993 Taken together, these results indicate that inhibition of p34cdc2 kinase activity by phosphorylation of Tyr-15 (within the putative ATP binding domain) or by mutation of Thr-161 involves a mechanism other than inhibition of nucleotide binding. Tyrosine 104-107 cyclin dependent kinase 1 Homo sapiens 58-65 8440392-1 1993 In mammalian cells, p34cdc2 kinase undergoes phosphorylation at threonine-14, tyrosine-15 and threonine-161 in the S and G2 phases of the cell cycle. Tyrosine 78-86 cyclin dependent kinase 1 Homo sapiens 20-27 8440392-0 1993 Dephosphorylation of human p34cdc2 kinase on both Thr-14 and Tyr-15 by human cdc25B phosphatase. Tyrosine 61-64 cyclin dependent kinase 1 Homo sapiens 27-34 8441405-1 1993 The kinase activity of human p34cdc2 is negatively regulated by phosphorylation at Thr-14 and Tyr-15. Tyrosine 94-97 cyclin dependent kinase 1 Homo sapiens 29-36 8441405-7 1993 Tyrosine-phosphorylated p34cdc2 was modified by FSBA with a slightly higher Km (241 microM FSBA). Tyrosine 0-8 cyclin dependent kinase 1 Homo sapiens 24-31 7678776-4 1993 P-Tyr incubation led to activation of cellular protein tyrosine phosphatases resulting in the inhibition of tyrosine phosphorylation of epidermal growth factor receptor as well as of p34cdc2. Tyrosine 55-63 cyclin dependent kinase 1 Homo sapiens 183-190 1384126-5 1992 The human WEE1 kinase phosphorylated the p34cdc2-cyclin B complex on tyrosine 15 but not on threonine 14 in vitro and inactivated the p34cdc2-cyclin B kinase. Tyrosine 69-77 cyclin dependent kinase 1 Homo sapiens 41-48 1384126-5 1992 The human WEE1 kinase phosphorylated the p34cdc2-cyclin B complex on tyrosine 15 but not on threonine 14 in vitro and inactivated the p34cdc2-cyclin B kinase. Tyrosine 69-77 cyclin dependent kinase 1 Homo sapiens 134-141 1379736-4 1992 We conclude that p34cdc2 partially activates pp60c-src by a two-step process in which its serine/threonine phosphorylations either sensitize pp60c-src to a Tyr-527 phosphatase or desensitize it to a Tyr-527 kinase. Tyrosine 156-159 cyclin dependent kinase 1 Homo sapiens 17-24 1379736-4 1992 We conclude that p34cdc2 partially activates pp60c-src by a two-step process in which its serine/threonine phosphorylations either sensitize pp60c-src to a Tyr-527 phosphatase or desensitize it to a Tyr-527 kinase. Tyrosine 199-202 cyclin dependent kinase 1 Homo sapiens 17-24 1320360-2 1992 In late G2, it is present as an inactive complex of tyrosine-phosphorylated p34cdc2 and unphosphorylated cyclin Bcdc13. Tyrosine 52-60 cyclin dependent kinase 1 Homo sapiens 76-83 1320360-3 1992 In M phase, its activation as an active MPF displaying histone H1 kinase activity originates from the specific tyrosine dephosphorylation of the p34cdc2 subunit by the tyrosine phosphatase p80cdc25. Tyrosine 111-119 cyclin dependent kinase 1 Homo sapiens 145-152 1483352-3 1992 Avian p34cdc2 is phosphorylated on threonine 14 (Thr14), tyrosine 15 (Tyr15), threonine 161 (Thr161) and serine 277 (Ser277). Tyrosine 57-65 cyclin dependent kinase 1 Homo sapiens 6-13 1551112-0 1992 Inhibition of p34cdc2 kinase activation, p34cdc2 tyrosine dephosphorylation, and mitotic progression in Chinese hamster ovary cells exposed to etoposide. Tyrosine 49-57 cyclin dependent kinase 1 Homo sapiens 41-48 1551112-2 1992 In this study, etoposide is shown to cause inhibition of a specific p34cdc2 kinase activation pathway, that of tyrosine dephosphorylation. Tyrosine 111-119 cyclin dependent kinase 1 Homo sapiens 68-75 1551112-5 1992 Activation of the kinase and the onset of mitosis were accompanied by increased electrophoretic mobility and tyrosine dephosphorylation of the p34cdc2 protein. Tyrosine 109-117 cyclin dependent kinase 1 Homo sapiens 143-150 1551112-8 1992 These data suggest that mammalian cells express a DNA damage-responsive mechanism which controls mitotic progression at the level of p34cdc2 tyrosine dephosphorylation. Tyrosine 141-149 cyclin dependent kinase 1 Homo sapiens 133-140 1731250-3 1992 In the fission yeast Schizosaccharomyces pombe, mutations that interfere with the phosphorylation of tyrosine 15 (Y15) of p34cdc2, the protein kinase subunit of maturation promoting factor, accelerate the entry into mitosis and abolish the ability of unreplicated DNA to arrest cells in G2. Tyrosine 101-109 cyclin dependent kinase 1 Homo sapiens 122-129 1731250-4 1992 Because the tyrosine phosphorylation of p34cdc2 is conserved in S. pombe, Xenopus, chicken and human cells, the regulation of p34cdc2-Y15 phosphorylation could be a universal mechanism mediating the S-phase feedback control and regulating the initiation of mitosis. Tyrosine 12-20 cyclin dependent kinase 1 Homo sapiens 40-47 1731250-4 1992 Because the tyrosine phosphorylation of p34cdc2 is conserved in S. pombe, Xenopus, chicken and human cells, the regulation of p34cdc2-Y15 phosphorylation could be a universal mechanism mediating the S-phase feedback control and regulating the initiation of mitosis. Tyrosine 12-20 cyclin dependent kinase 1 Homo sapiens 126-133 1534688-8 1992 Inhibition was not due to decreased levels of cdc2 or cyclin B protein; rather, G2 arrest correlated with the accumulation of both tyrosine-phosphorylated cdc2 and cyclin B. Tyrosine 131-139 cyclin dependent kinase 1 Homo sapiens 155-159 1312900-4 1992 Both tyrosine dephosphorylation of p34cdc2 and cyclin B accumulation were altered. Tyrosine 5-13 cyclin dependent kinase 1 Homo sapiens 35-42 1961714-1 1991 The cdc25+ gene of fission yeast encodes a phosphotyrosine phosphatase that dephosphorylates tyrosine-15 of p34cdc2 and thereby activates p34cdc2/cyclin to bring about entry into M phase. Tyrosine 50-58 cyclin dependent kinase 1 Homo sapiens 108-115 1961714-1 1991 The cdc25+ gene of fission yeast encodes a phosphotyrosine phosphatase that dephosphorylates tyrosine-15 of p34cdc2 and thereby activates p34cdc2/cyclin to bring about entry into M phase. Tyrosine 50-58 cyclin dependent kinase 1 Homo sapiens 138-145 1936263-4 1991 Its ability to phosphorylate casein but not histone, and its phosphorylation on tyrosine, detected by anti-phosphotyrosine antibodies, indicates the form of p34cdc2 purified is the inactive or apoenzyme form. Tyrosine 80-88 cyclin dependent kinase 1 Homo sapiens 157-164 1830667-8 1991 In hamster cells, the hyperphosphorylated form of p34cdc2 was complexed with cyclin B and underwent tyrosine dephosphorylation during caffeine-induced premature mitosis. Tyrosine 100-108 cyclin dependent kinase 1 Homo sapiens 50-57 1655274-1 1991 Genetic and biochemical studies have indicated that the cdc25 protein controls the entry into mitosis by triggering tyrosine dephosphorylation of the cdc2 protein kinase. Tyrosine 116-124 cyclin dependent kinase 1 Homo sapiens 56-60 1828290-1 1991 Oocytes arrested in the G2 phase of the cell cycle contain a p34cdc2/cyclin B complex which is kept in an inactive form by phosphorylation of its p34cdc2 subunit on tyrosine, threonine and perhaps serine residues. Tyrosine 165-173 cyclin dependent kinase 1 Homo sapiens 61-68 1664235-2 1991 Recent studies have shown that p34cdc2 is also a major tyrosine-phosphorylated protein in HeLa cells and that its phosphotyrosine content is cell cycle regulated and related to its kinase activity. Tyrosine 55-63 cyclin dependent kinase 1 Homo sapiens 31-38 1664235-4 1991 Tyrosine phosphorylation of cdc2 in vitro occurs at tyrosine 15, the same site that is phosphorylated in vivo. Tyrosine 0-8 cyclin dependent kinase 1 Homo sapiens 28-32 1664235-4 1991 Tyrosine phosphorylation of cdc2 in vitro occurs at tyrosine 15, the same site that is phosphorylated in vivo. Tyrosine 52-60 cyclin dependent kinase 1 Homo sapiens 28-32 1828701-7 1991 Erbstatin, AG18 and AG125 prevented 1-methyladenine-induced tyrosine dephosphorylation of p34cdc2, and they inhibited tyrosine phosphorylation of p44mpk. Tyrosine 60-68 cyclin dependent kinase 1 Homo sapiens 90-97 1828290-1 1991 Oocytes arrested in the G2 phase of the cell cycle contain a p34cdc2/cyclin B complex which is kept in an inactive form by phosphorylation of its p34cdc2 subunit on tyrosine, threonine and perhaps serine residues. Tyrosine 165-173 cyclin dependent kinase 1 Homo sapiens 146-153 1850698-0 1991 Cyclin promotes the tyrosine phosphorylation of p34cdc2 in a wee1+ dependent manner. Tyrosine 20-28 cyclin dependent kinase 1 Homo sapiens 48-55 1850698-3 1991 Co-production of p34cdc2 and p107wee1 in insect cells resulted in a minor population of p34cdc2 that was phosphorylated on tyrosine and displayed an altered electrophoretic mobility. Tyrosine 123-131 cyclin dependent kinase 1 Homo sapiens 17-24 1850698-3 1991 Co-production of p34cdc2 and p107wee1 in insect cells resulted in a minor population of p34cdc2 that was phosphorylated on tyrosine and displayed an altered electrophoretic mobility. Tyrosine 123-131 cyclin dependent kinase 1 Homo sapiens 88-95 1850698-4 1991 When p34cdc2 and p107wee1 were co-produced with cyclin (A or B) in insect cells, there was a dramatic increase in the population of p34cdc2 that was phosphorylated on tyrosine and that displayed a shift in electrophoretic mobility. Tyrosine 167-175 cyclin dependent kinase 1 Homo sapiens 5-12 1850698-4 1991 When p34cdc2 and p107wee1 were co-produced with cyclin (A or B) in insect cells, there was a dramatic increase in the population of p34cdc2 that was phosphorylated on tyrosine and that displayed a shift in electrophoretic mobility. Tyrosine 167-175 cyclin dependent kinase 1 Homo sapiens 132-139 1850698-5 1991 The phosphorylation of p34cdc2 on tyrosine was absolutely dependent upon the presence of kinase-active p107wee1. Tyrosine 34-42 cyclin dependent kinase 1 Homo sapiens 23-30 1850698-7 1991 These results suggest that cyclin functions to facilitate tyrosine phosphorylation of p34cdc2 and that p107wee1 functions to regulate p34cdc2, either directly or indirectly, by tyrosine phosphorylation. Tyrosine 58-66 cyclin dependent kinase 1 Homo sapiens 86-93 1850698-7 1991 These results suggest that cyclin functions to facilitate tyrosine phosphorylation of p34cdc2 and that p107wee1 functions to regulate p34cdc2, either directly or indirectly, by tyrosine phosphorylation. Tyrosine 177-185 cyclin dependent kinase 1 Homo sapiens 134-141 10588655-6 1999 This correlated with elevated Cdc2 tyrosine 15 phosphorylation, decreased Cdc2 activity, and decreased Cdc25C protein levels. Tyrosine 35-43 cyclin dependent kinase 1 Homo sapiens 30-34 2155162-5 1990 The p34cdc2 subunit becomes tyrosine-dephosphorylated as the H1K is activated during entry of the oocytes into M phase, whereas the cyclin subunit is reciprocally phosphorylated. Tyrosine 28-36 cyclin dependent kinase 1 Homo sapiens 4-11 32277442-9 2020 When the cells were synchronized and treated with gemcitabine at G2/M transition, the degradation of cyclin B1 and dephosphorylation of cdc-2 at Tyr 15 residue did not occur, resulting in cycle arrest. Tyrosine 145-148 cyclin dependent kinase 1 Homo sapiens 136-141 35056723-8 2022 The molecular mechanism of action of low-dose 7HF in TNBC cells primarily involved G2/M-phase arrest through upregulation of the expression of Bub3, cyclin B1, phosphorylated Cdk1 (Tyr 15) and p53-independent p21. Tyrosine 181-184 cyclin dependent kinase 1 Homo sapiens 175-179 2462672-3 1988 One site of cdc2 tyrosine phosphorylation in vivo is selectively phosphorylated by pp60c-src in vitro. Tyrosine 17-25 cyclin dependent kinase 1 Homo sapiens 12-16 33465289-3 2021 v-Src directly phosphorylates Tyr-15 of cyclin-dependent kinase 1 (CDK1), thereby causing mitotic slippage and reduction in Eg5 inhibitor cytotoxicity. Tyrosine 30-33 cyclin dependent kinase 1 Homo sapiens 40-65 33465289-3 2021 v-Src directly phosphorylates Tyr-15 of cyclin-dependent kinase 1 (CDK1), thereby causing mitotic slippage and reduction in Eg5 inhibitor cytotoxicity. Tyrosine 30-33 cyclin dependent kinase 1 Homo sapiens 67-71 31760882-7 2020 The amount of repressive phosphorylation of CDK1 at tyrosine 15 (Y15) was decreased by Chk1 inhibitor treatment. Tyrosine 52-60 cyclin dependent kinase 1 Homo sapiens 44-48 31914610-10 2020 Furthermore, we observed that the UFM1 cascade could alter the level of phosphorylation on tyrosine-15 of CDK1 (pY15-CDK1), which is a negative regulator of the G2 to M transition. Tyrosine 91-99 cyclin dependent kinase 1 Homo sapiens 106-110 31914610-10 2020 Furthermore, we observed that the UFM1 cascade could alter the level of phosphorylation on tyrosine-15 of CDK1 (pY15-CDK1), which is a negative regulator of the G2 to M transition. Tyrosine 91-99 cyclin dependent kinase 1 Homo sapiens 117-121 30135207-0 2018 The tyrosine kinase v-Src causes mitotic slippage by phosphorylating an inhibitory tyrosine residue of Cdk1. Tyrosine 4-12 cyclin dependent kinase 1 Homo sapiens 103-107 31285938-14 2019 Furthermore, the deactivated states of tyrosine phosphorylation of CDK1 (CDK1-Y15) and serine phosphorylation of Cdc25C (Cdc25C-S216) were upregulated after H. pylori infection. Tyrosine 39-47 cyclin dependent kinase 1 Homo sapiens 67-71 31285938-14 2019 Furthermore, the deactivated states of tyrosine phosphorylation of CDK1 (CDK1-Y15) and serine phosphorylation of Cdc25C (Cdc25C-S216) were upregulated after H. pylori infection. Tyrosine 39-47 cyclin dependent kinase 1 Homo sapiens 73-77 30754676-5 2019 In C/EBPbeta-knockdown cells, a prolonged increase in phosphorylation of cyclin dependent kinase 1 at tyrosine 15 (Y15-pCDK1) was displayed with simultaneously increased Wee1 and decreased Cdc25B expression. Tyrosine 102-110 cyclin dependent kinase 1 Homo sapiens 73-98 31200459-2 2019 By phosphorylating cdk1 at tyrosine 15, Wee1 inhibits activation of cyclin B-cdk1 (Cdk1), preventing cells from entering mitosis with incompletely replicated or damaged DNA. Tyrosine 27-35 cyclin dependent kinase 1 Homo sapiens 19-23 31200459-2 2019 By phosphorylating cdk1 at tyrosine 15, Wee1 inhibits activation of cyclin B-cdk1 (Cdk1), preventing cells from entering mitosis with incompletely replicated or damaged DNA. Tyrosine 27-35 cyclin dependent kinase 1 Homo sapiens 77-81 31200459-2 2019 By phosphorylating cdk1 at tyrosine 15, Wee1 inhibits activation of cyclin B-cdk1 (Cdk1), preventing cells from entering mitosis with incompletely replicated or damaged DNA. Tyrosine 27-35 cyclin dependent kinase 1 Homo sapiens 83-87 30135207-5 2018 Proteomic analysis and in vitro kinase assay revealed that v-Src phosphorylates cyclin-dependent kinase 1 (Cdk1) at Tyr-15. Tyrosine 116-119 cyclin dependent kinase 1 Homo sapiens 80-105 30135207-5 2018 Proteomic analysis and in vitro kinase assay revealed that v-Src phosphorylates cyclin-dependent kinase 1 (Cdk1) at Tyr-15. Tyrosine 116-119 cyclin dependent kinase 1 Homo sapiens 107-111 30135207-8 2018 These results suggest that v-Src causes mitotic slippage by attenuating Cdk1 kinase activity via direct phosphorylation of Cdk1 at Tyr-15. Tyrosine 131-134 cyclin dependent kinase 1 Homo sapiens 72-76 30135207-8 2018 These results suggest that v-Src causes mitotic slippage by attenuating Cdk1 kinase activity via direct phosphorylation of Cdk1 at Tyr-15. Tyrosine 131-134 cyclin dependent kinase 1 Homo sapiens 123-127 30067982-6 2018 Mechanistically, we found that MIG6 depletion results in reduced phosphorylation of CDK1 on the inhibitory WEE1-targeted tyrosine-15 residue. Tyrosine 121-129 cyclin dependent kinase 1 Homo sapiens 84-88 29754473-4 2018 We observed that de-polymerization of actin with cytochalasin D (CD) constitutively activated ribosomal S6 kinase (RSK) and induced inhibitory phosphorylation of Cdc2 (Tyr 15) in IMR-90 cells. Tyrosine 168-171 cyclin dependent kinase 1 Homo sapiens 162-166 29163782-4 2017 ATR subsequently phosphorylates H2AX at serine 139 (gammaH2AX) and CHK1 at serine 345 (CHK1pS345), leading to phosphorylation of CDK1 at tyrosine 15 (CDK1pY15) and S-phase arrest. Tyrosine 137-145 cyclin dependent kinase 1 Homo sapiens 129-133 29783721-4 2018 AZD1775 is a WEE1 inhibitor that overrides and opens the G2/M checkpoint by preventing WEE1-mediated phosphorylation of cdc2 at tyrosine 15. Tyrosine 128-136 cyclin dependent kinase 1 Homo sapiens 120-124 29606300-7 2018 All four mutations significantly decreased the amount of WEE2 protein in vitro and in affected individuals" oocytes in vivo, and they all led to abnormal serine phosphorylation of WEE2 and reduced tyrosine 15 phosphorylation of Cdc2 in vitro. Tyrosine 197-205 cyclin dependent kinase 1 Homo sapiens 228-232 25084614-9 2014 MK-1775 induced apoptosis in both AML cell lines and diagnostic blast samples, accompanied by decreased phosphorylation of CDK1 and CDK2 on Tyr-15 and increased DNA double-strand breaks (DSBs). Tyrosine 140-143 cyclin dependent kinase 1 Homo sapiens 123-127 27070276-7 2016 Furthermore, the timing of the inhibitory tyrosine phosphorylation of Cyclin Dependent Kinase 1 (CDK1) was altered in the OGA knockdown cells. Tyrosine 42-50 cyclin dependent kinase 1 Homo sapiens 70-95 27070276-7 2016 Furthermore, the timing of the inhibitory tyrosine phosphorylation of Cyclin Dependent Kinase 1 (CDK1) was altered in the OGA knockdown cells. Tyrosine 42-50 cyclin dependent kinase 1 Homo sapiens 97-101 27123550-5 2016 DPD could also induce the cell cycle arrest at G2/M phase in HeLa cells and significantly increase the phosphorylation (Tyr 15) of CDC2 leading to inactivation of CDC2. Tyrosine 120-123 cyclin dependent kinase 1 Homo sapiens 131-135 27123550-5 2016 DPD could also induce the cell cycle arrest at G2/M phase in HeLa cells and significantly increase the phosphorylation (Tyr 15) of CDC2 leading to inactivation of CDC2. Tyrosine 120-123 cyclin dependent kinase 1 Homo sapiens 163-167 26216057-3 2015 Chk1 blocks mitosis by maintaining high levels of inhibitory tyrosine phosphorylation of the mitotic cyclin-dependent kinase 1; however, the mechanisms that underlie replication fork stabilization and suppression of origin firing are less well defined. Tyrosine 61-69 cyclin dependent kinase 1 Homo sapiens 101-126 26131711-1 2015 The activity of Cdc2 (CDK1) kinase, which coordinates cell cycle progression and DNA break repair, is blocked upon its phosphorylation at tyrosine 15 (Y15) by Wee1 kinase in the presence of DNA damage. Tyrosine 138-146 cyclin dependent kinase 1 Homo sapiens 22-26 25994054-6 2015 Increased levels of cAMP and/or ROS trigger accumulation of Thr-14/Tyr-15 phosphorylated cyclin-dependent kinase 1 (Cdk1) on one hand and degradation of cyclin B1 through ubiquitin-mediated proteolysis on the other hand to destabilize maturation promoting factor (MPF). Tyrosine 67-70 cyclin dependent kinase 1 Homo sapiens 89-114 25994054-6 2015 Increased levels of cAMP and/or ROS trigger accumulation of Thr-14/Tyr-15 phosphorylated cyclin-dependent kinase 1 (Cdk1) on one hand and degradation of cyclin B1 through ubiquitin-mediated proteolysis on the other hand to destabilize maturation promoting factor (MPF). Tyrosine 67-70 cyclin dependent kinase 1 Homo sapiens 116-120 25435947-7 2015 Furthermore, western blot analysis revealed that the expression levels of phospho-cdc2 (Tyr 15), cyclin A2 and cyclin B1 were increased in a time-dependent manner, following the administration of CKD-602. Tyrosine 88-91 cyclin dependent kinase 1 Homo sapiens 82-86 26409447-6 2016 Examination of the cell cycle analysis showed that resveratrol treatment induced cell cycle arrest in the G2/M phase and enhanced the expression of phospho-cdc2 (Tyr 15), cyclin A2, and cyclin B1 in the oral squamous cell carcinoma (OSCC) cells. Tyrosine 162-165 cyclin dependent kinase 1 Homo sapiens 156-160 25872745-5 2015 In these cells, inhibitory phosphorylation of Cdc25C phosphatase (Ser(216)) and CDK1 (Tyr(15)) was elevated; consequently, cell-cycle progression was delayed at G2/M phase, suggesting that activation of the ATM-Chk2 pathway by the HBV X protein (HBx) induces cell-cycle delay. Tyrosine 86-89 cyclin dependent kinase 1 Homo sapiens 80-84 25732194-3 2015 Under these conditions, Cdk1 phosphorylation at Thr-161 and dephosphorylation at Tyr-15, up-regulation of cyclin B1 expression, histone H1 phosphorylation, Cdc25C phosphorylation at Thr-48, Bcl-2 phosphorylation at Thr-56 and Ser-70, Mcl-1 phosphorylation at Ser-159/Thr-163, and Bim phosphorylation were detected irrespective of Bcl-2 overexpression. Tyrosine 81-84 cyclin dependent kinase 1 Homo sapiens 24-28 25204801-5 2015 G-1 treatment also increased mitotic index concurrent with a decrease in phosphorylation of Cdk1 (Tyr 15) and an increase in phosphorylation of the mitotic checkpoint protein BuBR1. Tyrosine 98-101 cyclin dependent kinase 1 Homo sapiens 92-96 24798733-3 2014 Mitotic exit in Chk2-deficient cells correlates with reduced levels of Mps1 protein and increased Cdk1-tyrosine 15 inhibitory phosphorylation. Tyrosine 103-111 cyclin dependent kinase 1 Homo sapiens 98-102 24872417-5 2014 Furthermore, in contrast to Cdk1 and Cdk2, which are inhibited by phosphorylation at Tyr-15, the kinase activity of Cdk5 is reported to be stimulated when phosphorylated at Tyr-15 by Src family kinases or receptor-type tyrosine kinases. Tyrosine 85-88 cyclin dependent kinase 1 Homo sapiens 28-32 22580740-11 2012 Conversely, the treatment increased the phosphorylated Cdc2 at Thr(14)/Tyr(15) and Cdc25C at Ser(216), which led to a decrease in Cdc2-cyclin B1 activity. Tyrosine 71-74 cyclin dependent kinase 1 Homo sapiens 55-59 23671119-2 2013 Phosphorylation of threonine 14 (T14) and tyrosine 15 (Y15) inhibits Cdks and regulates their activities in numerous physiologic contexts. Tyrosine 42-50 cyclin dependent kinase 1 Homo sapiens 69-73 23397083-6 2013 This was associated with upregulated checkpoint kinase (CHK2) expression and reduced CDC2 activity (i.e., increased phosphorylation of CDC2 on Tyr-15 and decreased phosphorylation of CDC2 on Thr-161). Tyrosine 143-146 cyclin dependent kinase 1 Homo sapiens 135-139 23397083-6 2013 This was associated with upregulated checkpoint kinase (CHK2) expression and reduced CDC2 activity (i.e., increased phosphorylation of CDC2 on Tyr-15 and decreased phosphorylation of CDC2 on Thr-161). Tyrosine 143-146 cyclin dependent kinase 1 Homo sapiens 135-139