PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 25284607-7 2014 However these models did predict that the tBu modification of tyrosine would result in an extension of the side chain out of the peptide-binding groove up towards the TCR. Tyrosine 62-70 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 167-170 25482031-5 2014 Selective targeting of TGFbeta1 cytokine into TCR/CD28 signalling plasma membrane domains held back early response of TCR-proximal tyrosine phosphorylation and bead engulfment at activation sites. Tyrosine 131-139 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 46-49 25482031-5 2014 Selective targeting of TGFbeta1 cytokine into TCR/CD28 signalling plasma membrane domains held back early response of TCR-proximal tyrosine phosphorylation and bead engulfment at activation sites. Tyrosine 131-139 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 118-121 25492967-7 2014 Two of these phosphorylation-dependent binding partners, the kinase Itk (interleukin-2-inducible Tec kinase) and the adaptor protein TSAd (T cell-specific adaptor), promoted the TCR-dependent phosphorylation of Lck at Tyr(192). Tyrosine 218-221 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 178-181 24638062-5 2014 A CD3zeta YF mutant in which all six tyrosine residues in the immunoreceptor tyrosine-based activation motif are mutated to phenylalanines is degraded as efficiently as is wild type CD3zeta, further suggesting that TCR signaling-triggered tyrosine phosphorylation of CD3zeta is dispensable for LAPTM5-mediated degradation. Tyrosine 77-85 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 215-218 24638062-5 2014 A CD3zeta YF mutant in which all six tyrosine residues in the immunoreceptor tyrosine-based activation motif are mutated to phenylalanines is degraded as efficiently as is wild type CD3zeta, further suggesting that TCR signaling-triggered tyrosine phosphorylation of CD3zeta is dispensable for LAPTM5-mediated degradation. Tyrosine 77-85 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 215-218 24688028-5 2014 This association increased after TCR restimulation in a SAP-dependent manner, requiring both immunoreceptor tyrosine-based switch motifs in the NTB-A cytoplasmic tail. Tyrosine 108-116 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 33-36 23880762-6 2013 We report that overexpression of Mn-SOD enhances tyrosine phosphorylation of TCR-associated membrane proximal signal transduction molecules Lck, LAT, ZAP70, PLCgamma1, and SLP76 within 1 min of TCR cross-linking. Tyrosine 49-57 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 77-80 23880762-6 2013 We report that overexpression of Mn-SOD enhances tyrosine phosphorylation of TCR-associated membrane proximal signal transduction molecules Lck, LAT, ZAP70, PLCgamma1, and SLP76 within 1 min of TCR cross-linking. Tyrosine 49-57 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 194-197 23580664-1 2013 T-cell receptor (TCR) triggering results in a cascade of intracellular tyrosine phosphorylation events that ultimately leads to T-cell activation. Tyrosine 71-79 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 0-15 23901140-2 2013 The adaptor SLP-76 [Src homology 2 (SH2) domain-containing leukocyte protein of 76 kD] has two key tyrosine residues, Tyr(113) and Tyr(128), that mediate signaling downstream of the TCR. Tyrosine 99-107 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 182-185 23901140-2 2013 The adaptor SLP-76 [Src homology 2 (SH2) domain-containing leukocyte protein of 76 kD] has two key tyrosine residues, Tyr(113) and Tyr(128), that mediate signaling downstream of the TCR. Tyrosine 118-121 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 182-185 23901140-2 2013 The adaptor SLP-76 [Src homology 2 (SH2) domain-containing leukocyte protein of 76 kD] has two key tyrosine residues, Tyr(113) and Tyr(128), that mediate signaling downstream of the TCR. Tyrosine 131-134 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 182-185 23874337-3 2013 The detyrosinated MT array and TCR-induced tyrosine phosphorylation should coincide for centrosome polarization. Tyrosine 43-51 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 31-34 23686495-8 2013 The E2 region contained a predicted Lck substrate site, and substitution of an alanine or histidine for the tyrosine reversed TCR-signaling inhibition. Tyrosine 108-116 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 126-129 23580664-1 2013 T-cell receptor (TCR) triggering results in a cascade of intracellular tyrosine phosphorylation events that ultimately leads to T-cell activation. Tyrosine 71-79 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 17-20 23240581-1 2013 Engagement of the TCR (T-cell receptor) induces tyrosine phosphorylation of the LAT (linker for the activation of T-cells) adaptor, and thereby it recruits several cytosolic mediators for downstream signalling pathways. Tyrosine 48-56 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 18-21 23240581-1 2013 Engagement of the TCR (T-cell receptor) induces tyrosine phosphorylation of the LAT (linker for the activation of T-cells) adaptor, and thereby it recruits several cytosolic mediators for downstream signalling pathways. Tyrosine 48-56 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 23-38 22674786-6 2012 Our observations showed that activation of Jurkat or primary human T cells using H(2) O(2) or TCR-induced stimulation led to simultaneous phosphorylation of the activating tyrosine residue, Y394 and the inhibitory tyrosine residue, Y505 of p56(lck) . Tyrosine 172-180 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 94-97 23555234-2 2013 The T cell antigen receptor (TCR) on the surface of T cells is phosphorylated by the kinase Lck and dephosphorylated by the phosphatase CD45 on multiple immunoreceptor tyrosine-based activation motifs (ITAMs). Tyrosine 168-176 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 4-27 23555234-2 2013 The T cell antigen receptor (TCR) on the surface of T cells is phosphorylated by the kinase Lck and dephosphorylated by the phosphatase CD45 on multiple immunoreceptor tyrosine-based activation motifs (ITAMs). Tyrosine 168-176 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 29-32 22935418-8 2012 Moreover, enforced expression of SOCS3 inhibited tyrosine phosphorylation of zeta-associated protein kinase of 70 kD in TCR-stimulated human Jurkat T cells. Tyrosine 49-57 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 120-123 22674786-6 2012 Our observations showed that activation of Jurkat or primary human T cells using H(2) O(2) or TCR-induced stimulation led to simultaneous phosphorylation of the activating tyrosine residue, Y394 and the inhibitory tyrosine residue, Y505 of p56(lck) . Tyrosine 214-222 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 94-97 21189249-0 2011 T cell receptor (TCR)-induced tyrosine phosphorylation dynamics identifies THEMIS as a new TCR signalosome component. Tyrosine 30-38 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 17-20 22020291-1 2012 Signal transduction from the T cell antigen receptor (TCR)/CD3 complex involves six different immunoreceptor tyrosine-based activation motifs (ITAM) located within the cytoplasmic tails of the CD3 chains. Tyrosine 109-117 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 29-52 22020291-1 2012 Signal transduction from the T cell antigen receptor (TCR)/CD3 complex involves six different immunoreceptor tyrosine-based activation motifs (ITAM) located within the cytoplasmic tails of the CD3 chains. Tyrosine 109-117 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 54-57 22020291-2 2012 Each ITAM possesses two conserved tyrosine residues that can undergo phosphorylation upon TCR/CD3 crosslinking and become a docking site for SH2-containing effector molecules. Tyrosine 34-42 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 90-93 21189249-0 2011 T cell receptor (TCR)-induced tyrosine phosphorylation dynamics identifies THEMIS as a new TCR signalosome component. Tyrosine 30-38 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 91-94 21189249-2 2011 Using stable isotope labeling by amino acids in cell culture (SILAC)-based quantitative proteomics, we investigated the kinetics of signal propagation after TCR-induced protein tyrosine phosphorylation. Tyrosine 177-185 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 157-160 21167217-1 2011 The TCR signal transduction is initiated by the activation of Src-family kinases (SFK) which phosphorylate Immunoreceptor tyrosine-based activation motifs (ITAM) present in the intracellular parts of the T-cell receptor (TCR) signaling subunits. Tyrosine 122-130 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 4-7 21167217-1 2011 The TCR signal transduction is initiated by the activation of Src-family kinases (SFK) which phosphorylate Immunoreceptor tyrosine-based activation motifs (ITAM) present in the intracellular parts of the T-cell receptor (TCR) signaling subunits. Tyrosine 122-130 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 204-219 21167217-1 2011 The TCR signal transduction is initiated by the activation of Src-family kinases (SFK) which phosphorylate Immunoreceptor tyrosine-based activation motifs (ITAM) present in the intracellular parts of the T-cell receptor (TCR) signaling subunits. Tyrosine 122-130 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 221-224 20028775-4 2010 To address this issue, the role of individual signaling pathways in the phosphorylation of Pyk2 tyrosines 402 and 580 upon TCR activation was assessed in human T cells. Tyrosine 96-105 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 123-126 21152094-1 2010 BACKGROUND: One of the earliest activation events following stimulation of the T cell receptor (TCR) is the phosphorylation of the immunoreceptor tyrosine-based activation motifs (ITAMs) within the CD3-associated complex by the Src family kinase Lck. Tyrosine 146-154 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 79-94 21152094-1 2010 BACKGROUND: One of the earliest activation events following stimulation of the T cell receptor (TCR) is the phosphorylation of the immunoreceptor tyrosine-based activation motifs (ITAMs) within the CD3-associated complex by the Src family kinase Lck. Tyrosine 146-154 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 96-99 21152094-6 2010 TCR stimulation led to the co-precipitation of Lck with the transmembrane adaptor protein LAT (linker for activation of T cells), Erk-mediated phosphorylation of Lck and no detectable dephosphorylation of Lck inhibitory tyrosine. Tyrosine 220-228 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 0-3 21152094-7 2010 Strikingly, upon LAT knockdown in Hut-78 cells, we found that LAT promoted TCR-induced phosphorylation of Lck and Fyn activatory tyrosines, TCRzeta chain phosphorylation and Zap-70 activation. Tyrosine 129-138 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 75-78 20940326-2 2011 Upon TCR engagement, LAT becomes tyrosine-phosphorylated and thereby, recruits to the membrane several proteins implicated in the activation of downstream signaling pathways. Tyrosine 33-41 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 5-8 20381867-3 2010 In this study, the TCR-induced phosphorylation kinetics of Pyk2 tyrosines 402 and 580 were characterized in human T cells. Tyrosine 64-73 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 19-22 20028775-5 2010 In contrast to other receptor systems, the TCR-induced phosphorylation of Pyk2 tyrosines 402 and 580 was dependent on the Src family kinases, Fyn or Lck. Tyrosine 79-88 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 43-46 20028775-6 2010 Interestingly, the TCR-mediated phosphorylation of Pyk2 tyrosines 402 and 580 did not require Ca(2+) influx, ZAP-70 activation, actin cytoskeleton rearrangement, or PI3K function. Tyrosine 56-65 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 19-22 19609962-0 2009 Large-scale proteomic analysis of tyrosine-phosphorylation induced by T-cell receptor or B-cell receptor activation reveals new signaling pathways. Tyrosine 34-42 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 70-85 19672294-5 2009 CONCLUSIONS/SIGNIFICANCE: We propose here a model in which Vav functions primarily as a tyrosine phosphorylated linker-protein for TCR activation of T cells. Tyrosine 88-96 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 131-134 19609962-1 2009 Activation of the T-cell receptor (TCR) and that of the B-cell receptor (BCR) elicits tyrosine-phosphorylation of proteins that belongs to similar functional categories, but result in distinct cellular responses. Tyrosine 86-94 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 18-33 19609962-1 2009 Activation of the T-cell receptor (TCR) and that of the B-cell receptor (BCR) elicits tyrosine-phosphorylation of proteins that belongs to similar functional categories, but result in distinct cellular responses. Tyrosine 86-94 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 35-38 19609962-4 2009 Our results led to the identification of novel tyrosine-phosphorylated proteins and signaling pathways not previously implicated in immunoreceptor signal transduction, such as clathrin, zonula occludens 2, eukaryotic translation initiation factor 3, and RhoH, suggesting that TCR or BCR signaling may be linked to downstream processes such as endocytosis, cell adhesion, and translation. Tyrosine 47-55 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 276-279 19192391-6 2009 These results suggested that Lck regulated the activity of PKD2 by tyrosine phosphorylation, which in turn may have modulated the physiological functions of PKD2 during TCR-induced T cell activation. Tyrosine 67-75 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 169-172 19265538-6 2009 Tyrosine phosphorylation of CD3-zeta was defined by immunoprecipitation and western immunoblotting following activation by T cell receptor (TcR) cross-linking. Tyrosine 0-8 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 123-138 19265538-6 2009 Tyrosine phosphorylation of CD3-zeta was defined by immunoprecipitation and western immunoblotting following activation by T cell receptor (TcR) cross-linking. Tyrosine 0-8 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 140-143 17420479-6 2007 Likewise, SLP-76, particularly its N-terminal tyrosines, was required for TCR-induced tyrosine phosphorylation and activation of ITK but was not required for the phosphorylation or activation of ZAP-70. Tyrosine 46-55 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 74-77 19201869-3 2009 Dephosphorylation of Tyr(505) upon TCR engagement is supposed to lead to unfolding of the kinase structure and enhanced kinase activity. Tyrosine 21-24 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 35-38 18457880-5 2008 The inhibitory effect of PTP-PEST was associated with dephosphorylation the Lck kinase at its activation loop site (Y394), reduced early TCR-induced tyrosine phosphorylation, reduced ZAP-70 phosphorylation and inhibition of MAP kinase activation. Tyrosine 149-157 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 137-140 18056706-6 2008 Furthermore, we show that TCR-induced tyrosine phosphorylation is regulated by SH3 domain modulation of the FynT-PAG interaction in human primary T-cells. Tyrosine 38-46 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 26-29 17464179-0 2007 CD99 activates T cells via a costimulatory function that promotes raft association of TCR complex and tyrosine phosphorylation of TCR zeta. Tyrosine 102-110 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 130-133 18619870-5 2008 TCR downmodulation required the polyproline-tyrosine motifs and the ubiquitin-interacting motif of LAPTM5. Tyrosine 44-52 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 0-3 17579069-8 2007 Fourth, whereas full-length SHP-1 potently inhibits TCR-induced tyrosine phosphorylation of specific proteins, expression of a SHP-1-carrying mutation within the 6-mer motif does not. Tyrosine 64-72 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 52-55 17579069-9 2007 Additionally, although SHP-1 DeltaC was functionally inactive, the addition of the 6-mer motif restored its functionality in inhibiting TCR-induced tyrosine phosphorylation. Tyrosine 148-156 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 136-139 17420479-6 2007 Likewise, SLP-76, particularly its N-terminal tyrosines, was required for TCR-induced tyrosine phosphorylation and activation of ITK but was not required for the phosphorylation or activation of ZAP-70. Tyrosine 46-54 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 74-77 16980618-2 2006 These structures develop rapidly, in parallel with TCR-induced increases in tyrosine phosphorylation and cytosolic calcium, and are likely to contribute to TCR-proximal signaling. Tyrosine 76-84 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 51-54 17353368-5 2007 Interestingly, either S376A mutation or HPK-1 knockdown resulted in increased TCR-induced tyrosine phosphorylation of SLP-76 and phospholipase C-gamma1. Tyrosine 90-98 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 78-81 16980618-2 2006 These structures develop rapidly, in parallel with TCR-induced increases in tyrosine phosphorylation and cytosolic calcium, and are likely to contribute to TCR-proximal signaling. Tyrosine 76-84 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 156-159 16257509-4 2006 Lck plays a critical role in TCR signal transduction by phosphorylating the immuno-receptor tyrosine based activation motif (ITAM). Tyrosine 92-100 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 29-32 16751250-1 2006 We develop a model of tyrosine phosphorylation and activation of the T-cell receptor (TCR) by localization to regions of close membrane-membrane proximity (close contact) that physically exclude tyrosine phosphatases such as CD45. Tyrosine 22-30 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 69-84 16751250-1 2006 We develop a model of tyrosine phosphorylation and activation of the T-cell receptor (TCR) by localization to regions of close membrane-membrane proximity (close contact) that physically exclude tyrosine phosphatases such as CD45. Tyrosine 22-30 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 86-89 16162276-6 2005 Disruption of tyrosine kinase activity after TCR signals are maximally induced causes the immediate reversal of tyrosine phosphorylation as well as cytoskeletal disruption, as indicated by loss of cell spreading, adhesion and CTL degranulation. Tyrosine 14-22 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 45-48 16984412-6 2006 However, TCR-mediated NFAT activation and tyrosine phosphorylation of zeta-associated protein 70 (Zap-70) were inhibited in cells pre-stimulated by flagellin. Tyrosine 42-50 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 9-12 16339550-2 2005 Following engagement of TCR, the Zap70 undergoes phosphorylation on multiple tyrosine residues that are implicated in the regulation of its catalytic activity and interaction with signaling effector molecules downstream of the TCR. Tyrosine 77-85 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 24-27 16339550-2 2005 Following engagement of TCR, the Zap70 undergoes phosphorylation on multiple tyrosine residues that are implicated in the regulation of its catalytic activity and interaction with signaling effector molecules downstream of the TCR. Tyrosine 77-85 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 227-230 16339550-3 2005 We have shown previously that the CT10 regulator of kinase II (CrkII) adapter protein interacts with tyrosine-phosphorylated Zap70 in TCR-engaged T cells, and now extend these studies to show that Tyr315 in the Zap70 interdomain B region is the site of interaction with CrkII. Tyrosine 101-109 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 134-137 16014381-6 2005 The activation of tyrosine phosphorylation and ERK by TCR ligation at reduced temperature also occurred in human primary T cells. Tyrosine 18-26 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 54-57 16103752-5 2005 Rather than trigger the MAPK cascade, TCR-mediated stimulation activates proximal tyrosine kinases, which results in the phosphorylation of p38 on a noncanonical activating residue, Tyr-323. Tyrosine 182-185 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 38-41 15558067-4 2005 The recruitment of Nck and WASp depended on TCR-induced tyrosine phosphorylation and the LAT and SLP-76 adaptors. Tyrosine 56-64 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 44-47 16081782-4 2005 We demonstrate that in normal LPT global tyrosine phosphorylation upon TCR cross-linking or an increase in intracellular H2O2, an inhibitor of protein tyrosine phosphatases, is muted. Tyrosine 41-49 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 71-74 16081782-7 2005 When glutathione is depleted, TCR-induced LPT tyrosine phosphorylation rises to PBT levels. Tyrosine 46-54 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 30-33 15572591-7 2005 Analysis of the TCR-induced signaling cascade showed that imatinib substantially reduced tyrosine phosphorylation of ZAP70 and LAT in response to activation through the TCR. Tyrosine 89-97 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 16-19 15572591-7 2005 Analysis of the TCR-induced signaling cascade showed that imatinib substantially reduced tyrosine phosphorylation of ZAP70 and LAT in response to activation through the TCR. Tyrosine 89-97 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 169-172 15944285-4 2005 The protein complexes were not preformed, and a number of tyrosine-phosphorylated proteins were inducibly and specifically associated with the TCR/CD3 complex. Tyrosine 58-66 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 143-146 15489916-3 2004 Recruited SFKs phosphorylate TCR ITAMs (immunoreceptor tyrosine-based activation motifs) in the CD3 and zeta chains, which then serve as docking sites for Syk-family kinases. Tyrosine 55-63 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 29-32 15100278-5 2004 These mutations in the ZAP-70 interdomain B region also specifically affect CD3/TCR-mediated tyrosine phosphorylation of residues 171 and 191 in the cytoplasmic domain of the linker for activation of T cells (LAT) adapter protein. Tyrosine 93-101 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 80-83 15059847-2 2004 Lck-mediated tyrosine phosphorylation of signaling motifs (ITAMs) in the CD3-zeta subunits of the TCR is an initial step in the transduction of signaling cascades. Tyrosine 13-21 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 98-101 15059847-9 2004 Importantly, the presence of ZAP-70 positively regulates the TCR-induced tyrosine phosphorylation of Syk. Tyrosine 73-81 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 61-64 15134785-2 2004 The earliest TCR-evoked signalling steps, such as tyrosine phosphorylations, ras activation and induction of Ca(2+) fluxes, are initiated in the T-cell plasma membrane. Tyrosine 50-58 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 13-16 15100278-8 2004 Thus, these studies demonstrate that the interdomain B region of ZAP-70 regulates beta(1) integrin activation by the CD3/TCR via control of tyrosine phosphorylation of tyrosine residues 171 and 191 in the LAT cytoplasmic domain. Tyrosine 140-148 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 121-124 15100278-8 2004 Thus, these studies demonstrate that the interdomain B region of ZAP-70 regulates beta(1) integrin activation by the CD3/TCR via control of tyrosine phosphorylation of tyrosine residues 171 and 191 in the LAT cytoplasmic domain. Tyrosine 168-176 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 121-124 14552840-3 2003 Signaling processes are controlled by the ten immunoreceptor tyrosine-based activation motifs (ITAMs) present in the invariant chains of the TCR complex (TCR zeta and CD3-gamma, -delta and -epsilon ). Tyrosine 61-69 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 141-144 15286379-2 2004 T-cell receptor (TCR)/CD3-mediated stimulation of SLE T cells shows increased protein tyrosine phosphorylation of cellular proteins, with faster kinetics, heightened calcium response, and decreased interleukin (IL)-2 production. Tyrosine 86-94 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 0-15 15286379-2 2004 T-cell receptor (TCR)/CD3-mediated stimulation of SLE T cells shows increased protein tyrosine phosphorylation of cellular proteins, with faster kinetics, heightened calcium response, and decreased interleukin (IL)-2 production. Tyrosine 86-94 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 17-20 14745016-4 2004 Previously, we reported that prolonged exposure of T cell hybridoma clones to tumor necrosis factor alpha (TNF) induces nondeletional and reversible hyporesponsiveness to TCR engagement, associated with down-regulation of TCRzeta chain expression, impaired TCR/CD3 complex assembly, and attenuation of TCR-induced membrane proximal tyrosine phosphorylation. Tyrosine 332-340 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 171-174 14552840-3 2003 Signaling processes are controlled by the ten immunoreceptor tyrosine-based activation motifs (ITAMs) present in the invariant chains of the TCR complex (TCR zeta and CD3-gamma, -delta and -epsilon ). Tyrosine 61-69 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 154-157 12938237-7 2003 As a consequence, early tyrosine phosphorylation events as well as downstream signaling pathways leading to IL-2 gene expression induced by TCR were inhibited in anti-CD4 pretreated cells. Tyrosine 24-32 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 140-143 12665526-3 2003 While TCR-triggering resulted in transient dissociation of Csk from Cbp/PAG/rafts allowing TCR-induced tyrosine phosphorylation to occur, pretreatment with PGE(2) reduced Csk dissociation upon TCR triggering. Tyrosine 103-111 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 6-9 12665526-3 2003 While TCR-triggering resulted in transient dissociation of Csk from Cbp/PAG/rafts allowing TCR-induced tyrosine phosphorylation to occur, pretreatment with PGE(2) reduced Csk dissociation upon TCR triggering. Tyrosine 103-111 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 91-94 12665526-3 2003 While TCR-triggering resulted in transient dissociation of Csk from Cbp/PAG/rafts allowing TCR-induced tyrosine phosphorylation to occur, pretreatment with PGE(2) reduced Csk dissociation upon TCR triggering. Tyrosine 103-111 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 91-94 12496963-4 2003 Both basal Lck activity in unstimulated cells and the TCR-induced increase in tyrosine phosphorylation of the TCR zeta-chain and in Lck activity depend on the expression of CD45 with a large ectodomain. Tyrosine 78-86 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 54-57 12614351-0 2003 The formation and functions of the 21- and 23-kDa tyrosine-phosphorylated TCR zeta subunits. Tyrosine 50-58 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 74-77 12614351-4 2003 Following TCR stimulation, the TCR zeta subunit forms two tyrosine-phosphorylated intermediates of 21- and 23-kDa, respectively. Tyrosine 58-66 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 10-13 12614351-4 2003 Following TCR stimulation, the TCR zeta subunit forms two tyrosine-phosphorylated intermediates of 21- and 23-kDa, respectively. Tyrosine 58-66 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 31-34 12680588-7 2003 In addition, this mutation abolished TCR-associated tyrosine phosphorylation, consistent with the impairment of cell signaling. Tyrosine 52-60 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 37-40 12496963-4 2003 Both basal Lck activity in unstimulated cells and the TCR-induced increase in tyrosine phosphorylation of the TCR zeta-chain and in Lck activity depend on the expression of CD45 with a large ectodomain. Tyrosine 78-86 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 110-113 12486104-2 2002 Upon TCR engagement, LAT becomes rapidly tyrosine phosphorylated and then serves as a scaffold organizing a multicomponent complex that is indispensable for induction of further downstream steps of the signaling cascade. Tyrosine 41-49 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 5-8 12077257-0 2002 TCR engagement induces proline-rich tyrosine kinase-2 (Pyk2) translocation to the T cell-APC interface independently of Pyk2 activity and in an immunoreceptor tyrosine-based activation motif-mediated fashion. Tyrosine 36-44 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 0-3 12385028-5 2002 In line with these results, also TCR-induced tyrosine phosphorylation is not inhibited by dispersion of lipid rafts. Tyrosine 45-53 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 33-36 12077249-2 2002 Preincubation of T cells with soluble YHB.46 caused a general inhibition of TCR-stimulated protein tyrosine phosphorylation events, including a reduction in phosphorylation of p95(vav), linker for activation of T cells, and Src homology 2 domain-containing leukocyte protein of 76-kDa signaling molecules. Tyrosine 99-107 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 76-79 12077249-4 2002 Examination of the earliest initiation events of TCR signal transduction showed that YHB.46 inhibited TCR-zeta chain phosphorylation together with recruitment and tyrosine phosphorylation of the zeta-associated protein of 70-kDa tyrosine kinase, particularly at Tyr(319), as well as reduced recruitment of p56(lck) to the TCR-zeta and zeta-associated protein of 70-kDa complex. Tyrosine 163-171 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 49-52 12077249-4 2002 Examination of the earliest initiation events of TCR signal transduction showed that YHB.46 inhibited TCR-zeta chain phosphorylation together with recruitment and tyrosine phosphorylation of the zeta-associated protein of 70-kDa tyrosine kinase, particularly at Tyr(319), as well as reduced recruitment of p56(lck) to the TCR-zeta and zeta-associated protein of 70-kDa complex. Tyrosine 262-265 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 49-52 12351398-3 2002 We show that coengagement of T-cell receptor (TCR) and CD158a by tumor cells inhibited tyrosine phosphorylation of early signaling proteins ZAP-70 and LAT, lipid raft coalescence, and TCR/CD3 accumulation at the CTL/tumor cell interface. Tyrosine 87-95 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 29-44 12351398-3 2002 We show that coengagement of T-cell receptor (TCR) and CD158a by tumor cells inhibited tyrosine phosphorylation of early signaling proteins ZAP-70 and LAT, lipid raft coalescence, and TCR/CD3 accumulation at the CTL/tumor cell interface. Tyrosine 87-95 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 46-49 11262396-4 2001 We show that Vav2 is tyrosine-phosphorylated upon TCR stimulation and by co-expressed Src and Syk family kinases. Tyrosine 21-29 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 50-53 11724779-7 2002 However, although SLP-76 phosphorylation is normally induced, TCR engagement of mutant cells results in lower tyrosine phosphorylation of LAT but in higher tyrosine phosphorylation of Vav than in wild type cells. Tyrosine 110-118 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 62-65 11724779-7 2002 However, although SLP-76 phosphorylation is normally induced, TCR engagement of mutant cells results in lower tyrosine phosphorylation of LAT but in higher tyrosine phosphorylation of Vav than in wild type cells. Tyrosine 156-164 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 62-65 11806999-3 2002 This report shows that CD150 colocalizes with the T-cell receptor (TCR) following CD3 triggering in human peripheral blood T cells and is rapidly and reversibly tyrosine phosphorylated on TCR cross-linking. Tyrosine 161-169 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 50-65 11806999-3 2002 This report shows that CD150 colocalizes with the T-cell receptor (TCR) following CD3 triggering in human peripheral blood T cells and is rapidly and reversibly tyrosine phosphorylated on TCR cross-linking. Tyrosine 161-169 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 67-70 11806999-3 2002 This report shows that CD150 colocalizes with the T-cell receptor (TCR) following CD3 triggering in human peripheral blood T cells and is rapidly and reversibly tyrosine phosphorylated on TCR cross-linking. Tyrosine 161-169 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 188-191 12415729-5 2002 Cross-linking of either the TCR or rafts strongly induces specific tyrosine phosphorylation of the TCR in the rafts. Tyrosine 67-75 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 28-31 12415729-5 2002 Cross-linking of either the TCR or rafts strongly induces specific tyrosine phosphorylation of the TCR in the rafts. Tyrosine 67-75 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 99-102 12415729-7 2002 Our results indicate a mechanism whereby TCR engagement promotes aggregation of lipid rafts, which facilitates colocalization of signaling proteins including Lck, LAT, and the TCR, while excluding CD45, thereby potentiating protein tyrosine phosphorylation and downstream signaling. Tyrosine 232-240 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 41-44 12415729-7 2002 Our results indicate a mechanism whereby TCR engagement promotes aggregation of lipid rafts, which facilitates colocalization of signaling proteins including Lck, LAT, and the TCR, while excluding CD45, thereby potentiating protein tyrosine phosphorylation and downstream signaling. Tyrosine 232-240 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 176-179 11509608-7 2001 Finally, SAP specifically interacted with a 75-kDa tyrosine-phosphorylated protein upon TCR stimulation. Tyrosine 51-59 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 88-91 11514606-3 2001 Additional tyrosines (Tyrs) within ZAP-70 are phosphorylated that play both positive and negative regulatory roles in TCR function. Tyrosine 11-20 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 118-121 11514606-3 2001 Additional tyrosines (Tyrs) within ZAP-70 are phosphorylated that play both positive and negative regulatory roles in TCR function. Tyrosine 22-26 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 118-121 11514606-4 2001 Phosphorylation of Tyr residues (Tyrs 315 and 319) within the Interdomain B region of the ZAP-70 PTK plays important roles in the generation of second messengers after TCR engagement. Tyrosine 19-22 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 168-171 11514606-4 2001 Phosphorylation of Tyr residues (Tyrs 315 and 319) within the Interdomain B region of the ZAP-70 PTK plays important roles in the generation of second messengers after TCR engagement. Tyrosine 33-37 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 168-171 11390650-2 2001 In particular, TCR-induced tyrosine phosphorylation and activation of phospholipase C-gamma1 (PLC-gamma1), and the resultant TCR-inducible gene expression, depend on SLP-76. Tyrosine 27-35 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 15-18 11262396-5 2001 Using glutathione S-transferase fusion proteins, we observed that the Src homology 2 domain of Vav2 binds tyrosine-phosphorylated proteins from TCR-stimulated Jurkat T cell lysates, including c-Cbl and SLP-76. Tyrosine 106-114 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 144-147 11420038-3 2001 Whereas the inducible tyrosine phosphorylation of TCR zeta and ZAP-70 and the kinase activity of Lck were intact, phosphorylated LAT was rapidly dephosphorylated by raft targeting of activated SHP-1, leading to defects in LAT activation and subsequent downstream signaling events. Tyrosine 22-30 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 50-53 11752630-7 2000 LAT is a 36-kD transmembrane protein that becomes rapidly tyrosine-phosphorylated after TCR engagement. Tyrosine 58-66 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 88-91 11114301-6 2001 A mutant form of CD47 without its Ig domain that did not induce actin polymerization or localize to rafts still enhanced T cell receptor (TCR)-dependent tyrosine phosphorylation of PLCgamma and associated Ca(2+) signaling but did not augment IL-2 secretion. Tyrosine 153-161 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 121-136 11114301-6 2001 A mutant form of CD47 without its Ig domain that did not induce actin polymerization or localize to rafts still enhanced T cell receptor (TCR)-dependent tyrosine phosphorylation of PLCgamma and associated Ca(2+) signaling but did not augment IL-2 secretion. Tyrosine 153-161 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 138-141 11145647-4 2001 We demonstrate that Lck SH3 functions after initiation of TCR-induced tyrosine phosphorylation and concentration of transducers within rafts, to regulate the costimulation-dependent migration of rafts to the TCR contact site. Tyrosine 70-78 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 58-61 11145647-4 2001 We demonstrate that Lck SH3 functions after initiation of TCR-induced tyrosine phosphorylation and concentration of transducers within rafts, to regulate the costimulation-dependent migration of rafts to the TCR contact site. Tyrosine 70-78 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 208-211 11680012-4 2001 In these cell types, SLP-76 and LAT are required for signaling by immunoreceptor tyrosine-based activation motif(ITAM)-containing receptors, including the T cell receptor (TCR), the pre-TCR, the high-affinity Fc epsilon receptor, and the platelet GPVI collagen receptor. Tyrosine 81-89 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 155-170 11680012-4 2001 In these cell types, SLP-76 and LAT are required for signaling by immunoreceptor tyrosine-based activation motif(ITAM)-containing receptors, including the T cell receptor (TCR), the pre-TCR, the high-affinity Fc epsilon receptor, and the platelet GPVI collagen receptor. Tyrosine 81-89 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 172-175 10652356-4 2000 The Src family protein-tyrosine kinase, Lck, was critical in TCR-induced tyrosine phosphorylation of PKCtheta. Tyrosine 23-31 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 61-64 10975819-4 2000 The TCR complex contains multiple signaling motifs, known as immunoreceptor tyrosine-based activation motifs (ITAMs) that are required for T cell activation. Tyrosine 76-84 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 4-7 10708444-8 2000 The peptides targeted by SIV Nef, YNELNL and YSEIGMKGERRR, are portions of the first and second of three immunoreceptor tyrosine-based activation motifs which are important in signal transduction, thymocyte development, and TCR biogenesis. Tyrosine 120-128 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 224-227 10709104-8 2000 Consistent with these findings, CEA-specific CTL showed increased tyrosine phosphorylation of TCR signaling proteins ZAP-70 and TCR zeta chains in response to both peptides. Tyrosine 66-74 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 94-97 11034377-3 2000 We demonstrate that galectin-1 antagonizes TCR responses known to require costimulation and processive protein tyrosine phosphorylation, such as IL-2 production, but is permissive for TCR responses that only require partial TCR signals, such as IFN-gamma production, CD69 up-regulation, and apoptosis. Tyrosine 111-119 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 43-46 11017104-1 2000 The T cell receptor (TCR) zeta subunit contains three immunoreceptor tyrosine-based activation motifs (ITAMs) that translate effective extracellular ligand binding into intracellular signals by becoming phosphorylated into 21- and 23-kD forms. Tyrosine 69-77 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 4-19 11017104-1 2000 The T cell receptor (TCR) zeta subunit contains three immunoreceptor tyrosine-based activation motifs (ITAMs) that translate effective extracellular ligand binding into intracellular signals by becoming phosphorylated into 21- and 23-kD forms. Tyrosine 69-77 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 21-24 11017104-2 2000 We report here that the 21-kD form of TCR zeta is generated by phosphorylation of the tyrosines in the second and third ITAMs, whereas the 23-kD form is formed by the additional phosphorylation of the membrane-proximal ITAM tyrosines. Tyrosine 86-95 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 38-41 11017104-2 2000 We report here that the 21-kD form of TCR zeta is generated by phosphorylation of the tyrosines in the second and third ITAMs, whereas the 23-kD form is formed by the additional phosphorylation of the membrane-proximal ITAM tyrosines. Tyrosine 224-233 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 38-41 11005864-6 2000 Second, TCR stimulation of Lck(-), Fyn(hi) Jurkat cells induced strong tyrosine phosphorylation of Vav. Tyrosine 71-79 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 8-11 10748192-4 2000 We show that engagement of T cell antigen receptor (TCR)/CD3 complex in either Jurkat cells or peripheral blood lymphocytes stimulates phosphorylation of Ser(727) but not Tyr(701) of Stat1. Tyrosine 171-174 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 27-50 10748192-4 2000 We show that engagement of T cell antigen receptor (TCR)/CD3 complex in either Jurkat cells or peripheral blood lymphocytes stimulates phosphorylation of Ser(727) but not Tyr(701) of Stat1. Tyrosine 171-174 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 52-55 24383527-16 2000 Moreover, tyrosine phosphorylation of Cas-L is critical for T-cell receptor and beta1 integrin-induced T-lymphocyte migration. Tyrosine 10-18 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 60-75 10652356-3 2000 In the present study, we report that PKCtheta is tyrosine phosphorylated in Jurkat T cells upon TCR.CD3 activation. Tyrosine 49-57 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 96-99 10652356-10 2000 These results suggest that Lck plays an important role in tyrosine phosphorylation of PKCtheta, which may in turn modulate the physiological functions of PKCtheta during TCR-induced T cell activation. Tyrosine 58-66 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 170-173 10490968-0 1999 Tyrosine phosphorylation of Crk-associated substrate lymphocyte-type is a critical element in TCR- and beta 1 integrin-induced T lymphocyte migration. Tyrosine 0-8 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 94-97 10651924-5 2000 Analysis of intracellular protein tyrosine phosphorylation after cellular activation provided further evidence that SEBDelta61Y could transduce a signal via the Vbeta17 TCR. Tyrosine 34-42 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 169-172 10570288-4 1999 Removal of the Emt/Itk pleckstrin homology domain (DeltaPH-Emt/Itk) abrogates the association of the kinase with the cell membrane, as well as its activation-induced colocalization with the TCR complex and subsequent tyrosine phosphorylation. Tyrosine 217-225 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 190-193 10525547-9 1999 Thus, our results indicate a mechanism whereby TCR engagement promotes aggregation of lipid rafts, which facilitates colocalization of LCK, LAT, and the TCR whilst excluding CD45, thereby triggering protein tyrosine phosphorylation. Tyrosine 207-215 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 47-50 10525547-9 1999 Thus, our results indicate a mechanism whereby TCR engagement promotes aggregation of lipid rafts, which facilitates colocalization of LCK, LAT, and the TCR whilst excluding CD45, thereby triggering protein tyrosine phosphorylation. Tyrosine 207-215 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 153-156 10636924-1 2000 T cell antigen receptor (TCR) stimulation induces the tyrosine phosphorylation of several intracellular proteins including the protooncogene Vav1. Tyrosine 54-62 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 0-23 10636924-1 2000 T cell antigen receptor (TCR) stimulation induces the tyrosine phosphorylation of several intracellular proteins including the protooncogene Vav1. Tyrosine 54-62 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 25-28 10583414-7 1999 We propose that binding of ZAP-70 to the TCR promotes, through conformational changes, its extensive phosphorylation on tyrosine. Tyrosine 120-128 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 41-44 10490968-2 1999 Our previous studies showed that Cas-L is tyrosine-phosphorylated following the ligation of TCR and beta 1 integrins in T lymphocytes. Tyrosine 42-50 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 92-95 10458777-4 1999 Using the phosphotyrosine binding domains of the Shc adaptor and the Fyn kinase, which both participate in CD4 signaling, as baits, we show that CD4 induces tyrosine phosphorylation of a subset of the proteins phosphorylated in response to TCR/CD3 engagement. Tyrosine 17-25 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 240-243 10438919-1 1999 Stimulation of mature T cells with agonist ligands of the Ag receptor (TCR) causes rapid phosphorylation of tyrosine-based activation motifs in the intracellular portion of TCR-zeta and CD3 and activation of several intracellular signaling cascades. Tyrosine 108-116 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 71-74 10438919-1 1999 Stimulation of mature T cells with agonist ligands of the Ag receptor (TCR) causes rapid phosphorylation of tyrosine-based activation motifs in the intracellular portion of TCR-zeta and CD3 and activation of several intracellular signaling cascades. Tyrosine 108-116 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 173-176 10427998-8 1999 Here, tyrosine phosphorylation of Syk and ZAP-70 was detected after TCR but not after CD28 ligation. Tyrosine 6-14 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 68-71 10395678-5 1999 Our findings, obtained by using ZAP-70-negative Jurkat T cells, indicate that CD28 costimulation augments TCR-mediated T cell activation by increasing the ZAP-70-mediated Tyr phosphorylation of Vav. Tyrosine 171-174 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 106-109 10427972-6 1999 Cells with the naive phenotype were considerably more active in TCR-dependent tyrosine phosphorylation, both at an overall level and specifically in terms of TCR-zeta and ZAP-70 phosphorylation. Tyrosine 78-86 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 64-67 10427981-3 1999 All chimeric TCR could be expressed stably at the cell surface and induce Ca2+ mobilization as well as phosphorylation of several protein substrates on tyrosine residues. Tyrosine 152-160 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 13-16 10427998-10 1999 The concurrent ligation of CD28 and TCR increased tyrosine phosphorylation of LAT. Tyrosine 50-58 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 36-39 10427998-7 1999 Previous studies implicated the protein tyrosine kinases ZAP-70 and Syk in the TCR-induced tyrosine phosphorylation of LAT. Tyrosine 40-48 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 79-82 10037717-0 1999 Tyrosine 319, a newly identified phosphorylation site of ZAP-70, plays a critical role in T cell antigen receptor signaling. Tyrosine 0-8 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 90-113 10360968-2 1999 Upon TCR engagement, LAT becomes phosphorylated on multiple tyrosine residues and then binds several critical signaling molecules. Tyrosine 60-68 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 5-8 10209031-1 1999 The first 10 residues within the Src homology domain (SH)-4 domain of the Src family kinase Fyn are required for binding to the immune receptor tyrosine-based activation motif (ITAM) of T cell receptor (TCR) subunits. Tyrosine 144-152 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 186-201 10209031-1 1999 The first 10 residues within the Src homology domain (SH)-4 domain of the Src family kinase Fyn are required for binding to the immune receptor tyrosine-based activation motif (ITAM) of T cell receptor (TCR) subunits. Tyrosine 144-152 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 203-206 10087161-4 1999 A-125800 blocked activation-dependent TCR tyrosine phosphorylation and intracellular calcium mobilization in Jurkat T cells (IC50 = 35 microM) and blocked T cell proliferation in response to alloantigen (IC50 = 14 microM) and CD3/CD28-induced IL-2 secretion (IC50 = 2.2 microM) in primary T cell cultures. Tyrosine 42-50 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 38-41 10068651-5 1999 Gab1 is phosphorylated on tyrosine upon stimulation through the thrombopoietin receptor (TPOR), stem cell factor receptor (SCFR), and T-cell and B-cell antigen receptors (TCR and BCR, respectively), in addition to IL-3R and gp130. Tyrosine 26-34 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 171-174 10068651-6 1999 Tyrosine phosphorylation of Gab2 was induced by stimulation through gp130, IL-2R, IL-3R, TPOR, SCFR, and TCR. Tyrosine 0-8 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 105-108 10037717-1 1999 Following T cell antigen receptor (TCR) engagement, the protein tyrosine kinase (PTK) ZAP-70 is rapidly phosphorylated on several tyrosine residues, presumably by two mechanisms: an autophosphorylation and a trans-phosphorylation by the Src-family PTK Lck. Tyrosine 64-72 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 10-33 10037717-1 1999 Following T cell antigen receptor (TCR) engagement, the protein tyrosine kinase (PTK) ZAP-70 is rapidly phosphorylated on several tyrosine residues, presumably by two mechanisms: an autophosphorylation and a trans-phosphorylation by the Src-family PTK Lck. Tyrosine 64-72 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 35-38 9710204-3 1998 However, the sites on Shc that are tyrosine phosphorylated in response to TCR engagement and the ability of different T cell tyrosine kinases to phosphorylate Shc have not been defined. Tyrosine 35-43 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 74-77 9886389-7 1999 In contrast, SDF-1 treatment resulted in a reduction of tyrosine phosphorylation of the TCR downstream effectors, ZAP-70, SLP-76, and LAT (linker for activation of T cells), suggesting that this chemokine potentially regulates the threshold for T cell activation. Tyrosine 56-64 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 88-91 9881969-6 1998 CD48/TCR coengagement qualitatively and quantitatively enhances lipid raft-dependent zeta association with the actin cytoskeleton and zeta tyrosine phosphorylation. Tyrosine 139-147 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 5-8 9922123-2 1998 Signals mediated through the T-cell receptor (TCR)/CD3 complex are associated with tyrosine phosphorylations of zeta-chains by the regulated activities of protein tyrosine kinases p56(lck) and p59(fyn) as well as protein tyrosine phosphatases. Tyrosine 83-91 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 29-44 9922123-2 1998 Signals mediated through the T-cell receptor (TCR)/CD3 complex are associated with tyrosine phosphorylations of zeta-chains by the regulated activities of protein tyrosine kinases p56(lck) and p59(fyn) as well as protein tyrosine phosphatases. Tyrosine 83-91 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 46-49 9685404-0 1998 Tyrosine 474 of ZAP-70 is required for association with the Shc adaptor and for T-cell antigen receptor-dependent gene activation. Tyrosine 0-8 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 80-103 10037772-6 1999 Previous studies have shown that the T cell antigen receptor (TCR) induces tyrosine phosphorylation of Pyk2. Tyrosine 75-83 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 37-60 10037772-6 1999 Previous studies have shown that the T cell antigen receptor (TCR) induces tyrosine phosphorylation of Pyk2. Tyrosine 75-83 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 62-65 10037772-7 1999 In this report, the concurrent ligation of CD28 and TCR increased tyrosine phosphorylation of Pyk2; however, the extent of phosphorylation by both receptors was equivalent to the sum of that induced by each receptor alone. Tyrosine 66-74 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 52-55 10037772-8 1999 The Syk/Zap inhibitor piceatannol blocked CD28, and TCR induced tyrosine phosphorylation of Pyk2, suggesting that Syk/Zap is involved in Pyk2 phosphorylation. Tyrosine 64-72 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 52-55 10037772-9 1999 In contrast, the phosphatidylinositol 3-kinase inhibitor wortmannin blocked TCR- but not CD28-induced phosphorylation of Pyk2, suggesting that CD28 and TCR activate distinct pathways to induce tyrosine phosphorylation of Pyk2. Tyrosine 193-201 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 152-155 9880255-2 1999 STAT5 became immediately and transiently phosphorylated on tyrosine 694 in response to TCR stimulation. Tyrosine 59-67 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 87-90 9755165-6 1998 We observed rapid, TCR-dependent activation of Pak1 and TCR-inducible association of Pak1 with Nck, which was tyrosine phosphorylated following stimulation. Tyrosine 110-118 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 19-22 9755165-6 1998 We observed rapid, TCR-dependent activation of Pak1 and TCR-inducible association of Pak1 with Nck, which was tyrosine phosphorylated following stimulation. Tyrosine 110-118 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 56-59 9749527-3 1998 Upon stimulation with anti-CD8 mAb OKT8, CD8-zeta and CD8-Igalpha chimeric proteins induced tyrosine phosphorylation of various cytoplasmic substrates as seen in TcR stimulation. Tyrosine 92-100 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 162-165 9710204-4 1998 In this report, we show that during TCR signaling, the tyrosines Y239, Y240 and Y317 of Shc are the primary sites of tyrosine phosphorylation. Tyrosine 55-64 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 36-39 9710204-4 1998 In this report, we show that during TCR signaling, the tyrosines Y239, Y240 and Y317 of Shc are the primary sites of tyrosine phosphorylation. Tyrosine 55-63 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 36-39 9710204-5 1998 Mutation of all three tyrosines completely abolished tyrosine phosphorylation of Shc following TCR stimulation. Tyrosine 22-31 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 95-98 9710204-5 1998 Mutation of all three tyrosines completely abolished tyrosine phosphorylation of Shc following TCR stimulation. Tyrosine 22-30 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 95-98 9701029-5 1998 Since exon 7 spans the GTP/GDP binding site and N-terminal tyrosine in the third ITAM domain of TCR zeta chain, the transcript lacking exon 7 may be responsible for altered signal transduction via TCR in these SLE patients. Tyrosine 59-67 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 96-99 9657132-4 1998 Lymphocytes from both peaks displayed a phenotype of mature T cells together with characteristics of a constitutively activated state; that is, they 1) exhibited high levels of tyrosine-phosphorylated T cell receptor (TCR) zeta chain, 2) spontaneously secreted IL-2, 3) expressed activation specific cell surface markers, and 4) were unresponsive to in vitro stimuli. Tyrosine 177-185 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 218-221 9692882-4 1998 In both systems we show that inhibiting CD28/B7 interaction resulted in impaired TCR-induced tyrosine phosphorylation of the signal-transducing zeta chain and ZAP-70. Tyrosine 93-101 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 81-84 9692882-5 1998 Consistent with a blockade of TCR-proximal signaling events, Jurkat cells stimulated in the absence of CD28 ligation were found to have strongly diminished tyrosine phosphorylation of cellular substrates and downstream signaling pathways such as Ca2+/calcineurin, ERK/MAPK and JNK. Tyrosine 156-164 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 30-33 9647226-4 1998 One such substrate, phospholipase C (PLC)-gamma1, becomes tyrosine phosphorylated on CD3/TCR activation and mediates inositol triphosphate-dependent Ca2+ flux. Tyrosine 58-66 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 89-92 9584148-3 1998 Coengagement of TCR and CD4 results in an agonist signaling pattern with complete tyrosine phosphorylation of TCR subunits, and recruitment and activation of ZAP-70. Tyrosine 82-90 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 110-113 9565634-4 1998 Expression of the mutant phosphatase significantly inhibited TCR-induced activation of the extracellular-regulated kinase (ERK)-2 member of the mitogen-activated protein kinase (MAPK) family, but had no effect on TCR-zeta chain tyrosine phosphorylation or TCR-elicited Ca2+ transients. Tyrosine 228-236 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 61-64 9584148-3 1998 Coengagement of TCR and CD4 results in an agonist signaling pattern with complete tyrosine phosphorylation of TCR subunits, and recruitment and activation of ZAP-70. Tyrosine 82-90 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 16-19 9488454-5 1998 The P116 cell line displays severe defects in TCR-induced signaling functions, including protein tyrosine phosphorylation, intracellular Ca2+ mobilization, and interleukin-2 promoter-driven transcription. Tyrosine 97-105 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 46-49 9521074-0 1998 A novel 90-kDa tyrosine-phosphorylated protein associated with TCR complex in thymocytes. Tyrosine 15-23 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 63-66 9498738-4 1998 Thus, in these cells we observed increased phosphotransferase activity of Lck as well as constitutive tyrosine phosphorylation of the TCR-associated ZAP-70 kinase and expression of the related Syk protein tyrosine kinase. Tyrosine 102-110 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 134-137 9521074-7 1998 In a normal T cell population, thymocytes but not splenic T cells induced the tyrosine phosphorylation of p90 upon TCR cross-linking. Tyrosine 78-86 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 115-118 9521074-1 1998 Ligation of the TCR-CD3 complex initiates a cascade of tyrosine phosphorylation that results in T cell activation. Tyrosine 55-63 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 16-19 9521074-3 1998 We previously found that a 90-kDa protein was tyrosine phosphorylated upon TCR cross-linking and the induction of the phosphorylation was dependent on the structure of the CD3 complex. Tyrosine 46-54 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 75-78 9464824-0 1997 A dual participation of ZAP-70 and scr protein tyrosine kinases is required for TCR-induced tyrosine phosphorylation of Sam68 in Jurkat T cells. Tyrosine 47-55 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 80-83 9368189-5 1998 Surprisingly, the observed TCR-zeta and ZAP-70 tyrosine phosphorylations appear delayed compared to stimulation via CD3. Tyrosine 47-55 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 27-30 9570517-0 1998 The amino-terminal Src homology 2 domain of phospholipase C gamma 1 is essential for TCR-induced tyrosine phosphorylation of phospholipase C gamma 1. Tyrosine 97-105 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 85-88 9570517-1 1998 TCR engagement activates phospholipase C gamma 1 (PLC gamma 1) via a tyrosine phosphorylation-dependent mechanism. Tyrosine 69-77 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 0-3 9570517-4 1998 Mutation of the amino-terminal SH2 domain (SH2(N) domain) resulted in defective tyrosine phosphorylation of PLC gamma 1 in response to TCR/CD3 perturbation. Tyrosine 80-88 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 135-138 9394826-3 1997 CD6 becomes phosphorylated on tyrosine (Tyr) residues following stimulation through the T cell receptor (TCR) complex. Tyrosine 30-38 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 88-103 9394826-3 1997 CD6 becomes phosphorylated on tyrosine (Tyr) residues following stimulation through the T cell receptor (TCR) complex. Tyrosine 30-38 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 105-108 9394826-3 1997 CD6 becomes phosphorylated on tyrosine (Tyr) residues following stimulation through the T cell receptor (TCR) complex. Tyrosine 40-43 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 88-103 9394826-3 1997 CD6 becomes phosphorylated on tyrosine (Tyr) residues following stimulation through the T cell receptor (TCR) complex. Tyrosine 40-43 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 105-108 9394826-6 1997 We were able to demonstrate that of the nine Tyr residues in the cytoplasmic domain of the largest isoform CD6a, the two C-terminal Tyr residues (Tyr 629/662) are critical for the phosphorylation of CD6 following TCR cross-linking. Tyrosine 45-48 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 213-216 9394826-6 1997 We were able to demonstrate that of the nine Tyr residues in the cytoplasmic domain of the largest isoform CD6a, the two C-terminal Tyr residues (Tyr 629/662) are critical for the phosphorylation of CD6 following TCR cross-linking. Tyrosine 132-135 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 213-216 9394826-6 1997 We were able to demonstrate that of the nine Tyr residues in the cytoplasmic domain of the largest isoform CD6a, the two C-terminal Tyr residues (Tyr 629/662) are critical for the phosphorylation of CD6 following TCR cross-linking. Tyrosine 132-135 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 213-216 9295033-3 1997 In addition, down-regulation of CD4-associated TCR signaling resulted in diminished tyrosine phosphorylation of mitogen-activated protein kinase (MAPK), a serine threonine kinase which is critically involved in the regulation of transcription factors. Tyrosine 84-92 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 47-50 9312162-3 1997 We found that Itk is tyrosine-phosphorylated after TCR cross-linking and that this phosphorylation depends on the presence of functional Lck. Tyrosine 21-29 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 51-54 9278318-0 1997 The Lck SH2 phosphotyrosine binding site is critical for efficient TCR-induced processive tyrosine phosphorylation of the zeta-chain and IL-2 production. Tyrosine 19-27 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 67-70 9278318-2 1997 This is in part due to its enzymatic activity as a tyrosine kinase responsible for TCR-induced tyrosine phosphorylation of zeta and CD3 receptor subunits. Tyrosine 51-59 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 83-86 9278318-4 1997 It has been proposed that phosphotyrosine binding by the Lck SH2 domain may enhance substrate tyrosine phosphorylation by facilitating the processive phosphorylation of multiple sites within the TCR complex. Tyrosine 33-41 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 195-198 9278318-7 1997 Here we demonstrate that mutation of amino acids important for SH2 phosphotyrosine binding significantly compromises the ability of F505 to enhance TCR-mediated protein tyrosine phosphorylation and Ag-induced IL-2 production in BI-141. Tyrosine 74-82 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 148-151 9305640-11 1997 The transmembrane LCK chimera also supported the induction of tyrosine phosphorylation and Ca2+ flux by the TCR in JCam-1.6 cells. Tyrosine 62-70 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 108-111 9281504-3 1997 ITAMs consist of paired tyrosine and leucine residues and play a central role in signal transduction of the BCR and the T-cell receptor (TCR). Tyrosine 24-32 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 120-135 9281504-3 1997 ITAMs consist of paired tyrosine and leucine residues and play a central role in signal transduction of the BCR and the T-cell receptor (TCR). Tyrosine 24-32 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 137-140 9295052-8 1997 Association between Crk and tyrosine-phosphorylated CasL after TcR stimulation was also confirmed in vivo. Tyrosine 28-36 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 63-66 9295033-5 1997 By contrast, cross-linking of the TCR/CD3 complex with the CD28 receptor improved tyrosine phosphorylation of MAPK and salvaged infected PBL from activation-induced cell death. Tyrosine 82-90 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 34-37 9295052-0 1997 Ligation of the T cell antigen receptor induces tyrosine phosphorylation of p105CasL, a member of the p130Cas-related docking protein family, and its subsequent binding to the Src homology 2 domain of c-Crk. Tyrosine 48-56 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 16-39 9200439-2 1997 The earliest detectable event in T cell activation, within seconds of TCR ligand recognition, is tyrosine phosphorylation of TCR subunits. Tyrosine 97-105 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 70-73 9200439-2 1997 The earliest detectable event in T cell activation, within seconds of TCR ligand recognition, is tyrosine phosphorylation of TCR subunits. Tyrosine 97-105 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 125-128 9200439-6 1997 Loss of Ag-induced tyrosine phosphorylation was not due to lack of Ag presentation, loss of TCR expression, or T cell death, but, rather, it was associated with a lack of TCR subunit tyrosine phosphorylation. Tyrosine 19-27 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 92-95 9172452-2 1996 Csk is probably involved in the downregulation of TCR signaling by C-terminal tyrosine phosphorylation of Lck and Fyn, but the mechanism whereby Csk targets these Src family members is not known. Tyrosine 78-86 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 50-53 9199177-2 1997 ZAP-70 becomes phosphorylated and activated by LCK protein tyrosine kinase after interaction of its two NH2-terminal SH2 domains with tyrosine-phosphorylated subunits of the activated TCR. Tyrosine 59-67 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 184-187 9199177-8 1997 In addition, as the major increase in tyrosine phosphorylation induced by the TCR also occurs at the cell cortex (Ley, S.C., M. Marsh, C.R. Tyrosine 38-46 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 78-81 9120273-3 1997 The tyrosine-phosphorylated ZAP70 kinase exhibits a particular phenotype with low affinities for proteins at 21, 40, 60, and 120 kDa, proteins normally co-precipitated with ZAP70 after TCR/CD3 stimulation. Tyrosine 4-12 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 185-188 9079813-4 1997 Results demonstrate that in BW5147 wild-type TCR, tail-less zeta TCR, CD3epsilon, and TCRzeta transduce signals leading to tyrosine phosphorylation of similar sets of cellular substrates, including the receptor subunits, Fyn, ZAP-70, and phospholipase Cgamma1 (PLCgamma1). Tyrosine 123-131 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 45-48 9079813-4 1997 Results demonstrate that in BW5147 wild-type TCR, tail-less zeta TCR, CD3epsilon, and TCRzeta transduce signals leading to tyrosine phosphorylation of similar sets of cellular substrates, including the receptor subunits, Fyn, ZAP-70, and phospholipase Cgamma1 (PLCgamma1). Tyrosine 123-131 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 65-68 8955094-7 1996 The ZAP-70 mutant studied here could be phosphorylated on tyrosine when associated to the TCR zeta chain and was able to bind p56(lck). Tyrosine 58-66 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 90-93 8955094-9 1996 Our data are most consistent with a model in which recruitment to the TCR allows ZAP-70 autophosphorylation and binding to p56(lck), which in turn phosphorylates Tyr-492 and/or Tyr-493 with consequent up-regulation of the ZAP-70 kinase activity. Tyrosine 162-165 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 70-73 8955094-9 1996 Our data are most consistent with a model in which recruitment to the TCR allows ZAP-70 autophosphorylation and binding to p56(lck), which in turn phosphorylates Tyr-492 and/or Tyr-493 with consequent up-regulation of the ZAP-70 kinase activity. Tyrosine 177-180 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 70-73 9016871-4 1997 In addition, T cells exposed to wild-type ligand in the presence of anti-CD4 antibodies show a pattern of TCR signaling resembling that seen using partial agonists, with predominant accumulation of the p21 tyrosine-phosphorylated form of TCR-zeta, reduced tyrosine phosphorylation of CD3epsilon, and no detectable phosphorylation of ZAP-70. Tyrosine 206-214 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 238-241 9016871-4 1997 In addition, T cells exposed to wild-type ligand in the presence of anti-CD4 antibodies show a pattern of TCR signaling resembling that seen using partial agonists, with predominant accumulation of the p21 tyrosine-phosphorylated form of TCR-zeta, reduced tyrosine phosphorylation of CD3epsilon, and no detectable phosphorylation of ZAP-70. Tyrosine 256-264 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 238-241 8977253-5 1996 Proximal TCR-mediated signaling events, including intracellular calcium mobilization, inositol phosphate production, and tyrosine phosphorylation of phospholipase Cgamma1, were similarly inhibited by lovastatin, although global protein tyrosine kinase activity remained intact. Tyrosine 121-129 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 9-12 8906825-6 1996 Measurements of the binding affinities of these peptide/Ld complexes for the 2C TCR showed that at 4 degrees C only a Tyr substitution at this position retained high affinity for the TCR. Tyrosine 118-121 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 80-83 8906825-6 1996 Measurements of the binding affinities of these peptide/Ld complexes for the 2C TCR showed that at 4 degrees C only a Tyr substitution at this position retained high affinity for the TCR. Tyrosine 118-121 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 183-186 8814252-3 1996 We have previously shown that the interaction of the p56lck SH2 domain with zeta-associated, tyrosine phosphorylated ZAP-70 and Syk kinases leads to an enhanced association of CD4 with TCR/CD3/zeta complex after CD3 stimulation of Jurkat cells. Tyrosine 93-101 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 185-188 8900205-2 1996 We have demonstrated earlier that the TcR/CD3-induced activation of Fyn results in tyrosine phosphorylation of several Fyn-associated proteins, including a protein of 116 kDa. Tyrosine 83-91 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 38-41 8790400-4 1996 The unique pattern of early receptor-induced tyrosine phosphorylation events induced by partial agonists has led to the hypothesis that altered TCR signaling is directly responsible for the development of anergy. Tyrosine 45-53 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 144-147 9064333-1 1996 p95vav, the product of the vav protooncogene, has been implicated in the T cell receptor (TCR)-mediated signaling cascade p95vav is phosphorylated on tyrosine residues after TCR stimulation by anti-TCR/CD3 antibodies and possesses a number of landmark features of signaling molecules such as a putative guanine nucleotide exchange factor domain, a pleckstrin homology domain, and an Sre homology (SH) 2 and two SH3 domains, which provide the capacity to form multimeric signaling complexes. Tyrosine 150-158 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 174-177 9064333-1 1996 p95vav, the product of the vav protooncogene, has been implicated in the T cell receptor (TCR)-mediated signaling cascade p95vav is phosphorylated on tyrosine residues after TCR stimulation by anti-TCR/CD3 antibodies and possesses a number of landmark features of signaling molecules such as a putative guanine nucleotide exchange factor domain, a pleckstrin homology domain, and an Sre homology (SH) 2 and two SH3 domains, which provide the capacity to form multimeric signaling complexes. Tyrosine 150-158 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 174-177 9064333-1 1996 p95vav, the product of the vav protooncogene, has been implicated in the T cell receptor (TCR)-mediated signaling cascade p95vav is phosphorylated on tyrosine residues after TCR stimulation by anti-TCR/CD3 antibodies and possesses a number of landmark features of signaling molecules such as a putative guanine nucleotide exchange factor domain, a pleckstrin homology domain, and an Sre homology (SH) 2 and two SH3 domains, which provide the capacity to form multimeric signaling complexes. Tyrosine 150-158 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 73-88 9064333-1 1996 p95vav, the product of the vav protooncogene, has been implicated in the T cell receptor (TCR)-mediated signaling cascade p95vav is phosphorylated on tyrosine residues after TCR stimulation by anti-TCR/CD3 antibodies and possesses a number of landmark features of signaling molecules such as a putative guanine nucleotide exchange factor domain, a pleckstrin homology domain, and an Sre homology (SH) 2 and two SH3 domains, which provide the capacity to form multimeric signaling complexes. Tyrosine 150-158 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 90-93 8760790-3 1996 Here we show that ligation of T cell receptor (TCR) by alloantigen alone, which results in anergy, activates tyrosine phosphorylation of TCR zeta and its association with fyn. Tyrosine 109-117 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 30-45 8760790-3 1996 Here we show that ligation of T cell receptor (TCR) by alloantigen alone, which results in anergy, activates tyrosine phosphorylation of TCR zeta and its association with fyn. Tyrosine 109-117 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 47-50 8760790-3 1996 Here we show that ligation of T cell receptor (TCR) by alloantigen alone, which results in anergy, activates tyrosine phosphorylation of TCR zeta and its association with fyn. Tyrosine 109-117 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 137-140 8760790-4 1996 In contrast, TCR ligation in the presence of B7 costimulation, which results in productive immunity, activates tyrosine phosphorylation of TCR zeta and CD3 chains, which associate with activated lck and zeta-associated protein (ZAP) 70. Tyrosine 111-119 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 13-16 8760790-4 1996 In contrast, TCR ligation in the presence of B7 costimulation, which results in productive immunity, activates tyrosine phosphorylation of TCR zeta and CD3 chains, which associate with activated lck and zeta-associated protein (ZAP) 70. Tyrosine 111-119 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 139-142 8766558-0 1996 A tyrosine-phosphorylated 110-120-kDa protein associates with the C-terminal SH2 domain of phosphotyrosine phosphatase-1D in T cell receptor-stimulated T cells. Tyrosine 2-10 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 125-140 8766558-3 1996 Since TCR aggregation results in phosphorylation of critical immunoreceptor tyrosine-based activation motifs (ITAM) in the TCR zeta chain, a doubly tyrosine-phosphorylated synthetic peptide containing the membrane-proximal zeta chain ITAM (zeta p ITAM) was used to characterize TCR zeta-associated PTPases. Tyrosine 76-84 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 6-9 8766558-3 1996 Since TCR aggregation results in phosphorylation of critical immunoreceptor tyrosine-based activation motifs (ITAM) in the TCR zeta chain, a doubly tyrosine-phosphorylated synthetic peptide containing the membrane-proximal zeta chain ITAM (zeta p ITAM) was used to characterize TCR zeta-associated PTPases. Tyrosine 76-84 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 123-126 8766558-3 1996 Since TCR aggregation results in phosphorylation of critical immunoreceptor tyrosine-based activation motifs (ITAM) in the TCR zeta chain, a doubly tyrosine-phosphorylated synthetic peptide containing the membrane-proximal zeta chain ITAM (zeta p ITAM) was used to characterize TCR zeta-associated PTPases. Tyrosine 148-156 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 6-9 8766558-3 1996 Since TCR aggregation results in phosphorylation of critical immunoreceptor tyrosine-based activation motifs (ITAM) in the TCR zeta chain, a doubly tyrosine-phosphorylated synthetic peptide containing the membrane-proximal zeta chain ITAM (zeta p ITAM) was used to characterize TCR zeta-associated PTPases. Tyrosine 148-156 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 123-126 8766558-6 1996 TCR stimulation did not result in the tyrosine phosphorylation of PTP-1D but caused the rapid and transient tyrosine phosphorylation of a 110-120-kDa protein which associated selectively with the C-terminal SH2 domain of PTP-1D. Tyrosine 108-116 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 0-3 8626561-5 1996 We demonstrate that the SH2,3 region interacts specifically and directly with numerous tyrosine-phosphorylated molecules following TcR cross-linking, including constitutively with mitogen-activated protein kinase (MAPK)/extracellular-regulated kinase and inducibly with the zeta chain of the TcR. Tyrosine 87-95 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 131-134 8673706-1 1996 T cell antigen receptor (TCR) stimulation induces tyrosine phosphorylation of many intracellular proteins, including the proto-oncogene Vav, which is expressed exclusively in hematopoietic and trophoblast cells. Tyrosine 50-58 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 0-23 8673706-1 1996 T cell antigen receptor (TCR) stimulation induces tyrosine phosphorylation of many intracellular proteins, including the proto-oncogene Vav, which is expressed exclusively in hematopoietic and trophoblast cells. Tyrosine 50-58 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 25-28 8673706-6 1996 The Vav SH2 domain is required for this interaction and for TCR-mediated Vav tyrosine phosphorylation. Tyrosine 77-85 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 60-63 8647168-1 1996 Immunoglobulin family tyrosine-based activation motifs (ITAM), which define the conserved signaling sequence EX2YX2L/IX7YX2L/I, couple the T cell antigen receptor (TCR) to cellular proteins including protein tyrosine kinases (PTK) and adapter molecules. Tyrosine 22-30 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 139-162 8647168-1 1996 Immunoglobulin family tyrosine-based activation motifs (ITAM), which define the conserved signaling sequence EX2YX2L/IX7YX2L/I, couple the T cell antigen receptor (TCR) to cellular proteins including protein tyrosine kinases (PTK) and adapter molecules. Tyrosine 22-30 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 164-167 8845015-2 1996 We have previously shown that the extent of tyrosine phosphorylation of TCR zeta, CD3 gamma, and CD3 epsilon subunits in proliferating CD4-CD8+ T cells after TCR stimulation was decreased when compared to similarly stimulated naive T cells expressing the same TCR. Tyrosine 44-52 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 72-75 8845015-2 1996 We have previously shown that the extent of tyrosine phosphorylation of TCR zeta, CD3 gamma, and CD3 epsilon subunits in proliferating CD4-CD8+ T cells after TCR stimulation was decreased when compared to similarly stimulated naive T cells expressing the same TCR. Tyrosine 44-52 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 158-161 8845015-2 1996 We have previously shown that the extent of tyrosine phosphorylation of TCR zeta, CD3 gamma, and CD3 epsilon subunits in proliferating CD4-CD8+ T cells after TCR stimulation was decreased when compared to similarly stimulated naive T cells expressing the same TCR. Tyrosine 44-52 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 158-161 8845015-4 1996 In this study we determined whether kinases that bind tyrosine phosphorylated TCR zeta chain were differentially regulated in naive and proliferating cells. Tyrosine 54-62 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 78-81 8845015-6 1996 Using this method we found that both ZAP-70 and p72syk bound tyrosine phosphorylated TCR zeta very efficiently. Tyrosine 61-69 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 85-88 8647120-1 1996 Activation of resting T lymphocytes by ligands to the complex of T cell antigen receptor (TCR) and CD3 is initiated by a series of critical tyrosine phosphorylation and dephosphorylation events. Tyrosine 140-148 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 65-88 8647120-1 1996 Activation of resting T lymphocytes by ligands to the complex of T cell antigen receptor (TCR) and CD3 is initiated by a series of critical tyrosine phosphorylation and dephosphorylation events. Tyrosine 140-148 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 90-93 8626561-5 1996 We demonstrate that the SH2,3 region interacts specifically and directly with numerous tyrosine-phosphorylated molecules following TcR cross-linking, including constitutively with mitogen-activated protein kinase (MAPK)/extracellular-regulated kinase and inducibly with the zeta chain of the TcR. Tyrosine 87-95 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 292-295 8626561-8 1996 These interactions were specific as molecules known to be tyrosine-phosphorylated following TcR cross-linking, phospholipase C-gamma1 and Fyn, were not bound. Tyrosine 58-66 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 92-95 8626561-11 1996 In addition, the binding of Lck to the tyrosine-phosphorylated zeta chain of the TcR would serve to strengthen the interaction of the associated CD4 and the TcR complex, leading to increased avidity for the antigen-major histocompatibility protein complex. Tyrosine 39-47 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 81-84 8626561-11 1996 In addition, the binding of Lck to the tyrosine-phosphorylated zeta chain of the TcR would serve to strengthen the interaction of the associated CD4 and the TcR complex, leading to increased avidity for the antigen-major histocompatibility protein complex. Tyrosine 39-47 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 157-160 8700169-4 1996 We have previously shown that proliferating but not naive T cells were susceptible to apoptosis after TCR stimulation and that the tyrosine phosphorylation of TCR zeta, CD3 gamma, and CD3 epsilon in proliferating T cells was decreased after TCR stimulation. Tyrosine 131-139 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 159-162 8700169-4 1996 We have previously shown that proliferating but not naive T cells were susceptible to apoptosis after TCR stimulation and that the tyrosine phosphorylation of TCR zeta, CD3 gamma, and CD3 epsilon in proliferating T cells was decreased after TCR stimulation. Tyrosine 131-139 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 159-162 7499840-8 1995 Finally, in addition to the inhibition of IL-2 production, tyrosine phosphorylation of CD3 zeta-chains observed following TCR stimulation, one of the important early activation events, was markedly reduced by anti-TSA-1. Tyrosine 59-67 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 122-125 8627172-1 1996 Zeta-associated protein (ZAP)-70 is a cytoplasmic protein tyrosine required for T cell antigen receptor (TCR) signaling and development. Tyrosine 58-66 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 80-103 8627172-1 1996 Zeta-associated protein (ZAP)-70 is a cytoplasmic protein tyrosine required for T cell antigen receptor (TCR) signaling and development. Tyrosine 58-66 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 105-108 8627172-3 1996 ZAP-70 interacts with the TCR by binding to tyrosine-phosphorylated immunoreceptor tyrosine-based activation motifs (ITAMs) present in the invariant subunits of the TCR complex. Tyrosine 44-52 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 26-29 8627172-3 1996 ZAP-70 interacts with the TCR by binding to tyrosine-phosphorylated immunoreceptor tyrosine-based activation motifs (ITAMs) present in the invariant subunits of the TCR complex. Tyrosine 44-52 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 165-168 8627172-3 1996 ZAP-70 interacts with the TCR by binding to tyrosine-phosphorylated immunoreceptor tyrosine-based activation motifs (ITAMs) present in the invariant subunits of the TCR complex. Tyrosine 83-91 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 26-29 8627172-3 1996 ZAP-70 interacts with the TCR by binding to tyrosine-phosphorylated immunoreceptor tyrosine-based activation motifs (ITAMs) present in the invariant subunits of the TCR complex. Tyrosine 83-91 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 165-168 8627172-5 1996 Biochemical studies with the SH2(N+C) mutant showed that it also blocked early TCR signaling events, such as p95vav tyrosine phosphorylation, extracellular signal-regulated kinase 2 activation, and the association of a number of tyrosine-phosphorylated proteins with growth factor receptor-binding protein 2 (GRB2). Tyrosine 116-124 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 79-82 8627172-5 1996 Biochemical studies with the SH2(N+C) mutant showed that it also blocked early TCR signaling events, such as p95vav tyrosine phosphorylation, extracellular signal-regulated kinase 2 activation, and the association of a number of tyrosine-phosphorylated proteins with growth factor receptor-binding protein 2 (GRB2). Tyrosine 229-237 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 79-82 8627172-8 1996 These results indicate that tyrosine-phosphorylated ITAM is the target for the action of this dominant-negative mutant, suggesting that the assembly of a functional receptor signaling complex on ITAMs is a critical proximal TCR signaling event leading to downstream activation. Tyrosine 28-36 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 224-227 7761456-1 1995 Tyrosine phosphorylation of a 17-amino acid immunoreceptor tyrosine-based activation motif (ITAM), conserved in each of the signaling subunits of the T-cell antigen receptor (TCR), mediates the recruitment of ZAP-70 and syk protein-tyrosine kinases (PTKs) to the activated receptor. Tyrosine 0-8 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 150-173 8580068-0 1995 Tyrosine-phosphorylated forms of Ig beta, CD22, TCR zeta and HOSS are major ligands for tandem SH2 domains of Syk. Tyrosine 0-8 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 48-51 8580068-5 1995 In Jurkat T cells one of the SH2-binding proteins represents the tyrosine-phosphorylated TCR zeta chain. Tyrosine 65-73 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 89-92 7561679-4 1995 Moreover, the TCR on a thymocyte line derived from this patient can signal, and the homologous kinase Syk is present at high levels and is tyrosine phosphorylated after TCR stimulation. Tyrosine 139-147 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 14-17 7561679-4 1995 Moreover, the TCR on a thymocyte line derived from this patient can signal, and the homologous kinase Syk is present at high levels and is tyrosine phosphorylated after TCR stimulation. Tyrosine 139-147 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 169-172 7791764-1 1995 T-cell receptor (TCR) cross-linking increases tyrosine phosphorylation of multiple proteins, only a few of which have been identified. Tyrosine 46-54 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 0-15 7791764-1 1995 T-cell receptor (TCR) cross-linking increases tyrosine phosphorylation of multiple proteins, only a few of which have been identified. Tyrosine 46-54 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 17-20 7791764-5 1995 Grb2 antisera also precipitated p85 from serum-starved cells, while TCR activation increased p85 and tyrosine-phosphorylated Cbl but not Cbl protein in Grb2 immunocomplexes. Tyrosine 101-109 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 68-71 7539035-9 1995 We propose that the interaction of the p56lck SH2 domain with zeta-associated tyrosine-phosphorylated ZAP-70 and/or Syk enables CD4 to associate with antigen-stimulated TCR/CD3/zeta complexes. Tyrosine 78-86 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 169-172 7584152-3 1995 We show here that TCR ligation induces association of tyrosine-phosphorylated TCR zeta with the detergent-insoluble cell fraction. Tyrosine 54-62 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 18-21 7584152-3 1995 We show here that TCR ligation induces association of tyrosine-phosphorylated TCR zeta with the detergent-insoluble cell fraction. Tyrosine 54-62 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 78-81 7584152-5 1995 This microfilament association is specific to TCR-associated polypeptides containing at least one intact immunoreceptor tyrosine-based activation motif (ITAM). Tyrosine 120-128 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 46-49 7584153-0 1995 The same tyrosine-based inhibition motif, in the intracytoplasmic domain of Fc gamma RIIB, regulates negatively BCR-, TCR-, and FcR-dependent cell activation. Tyrosine 9-17 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 118-121 7584153-1 1995 The cell-triggering properties of BCR, TCR and FcR depend on structurally related immunoreceptor tyrosine-based activation motifs (ITAMs). Tyrosine 97-105 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 39-42 7584153-6 1995 The same tyrosine-based inhibitory motif (ITIM), which is highly conserved in murine and human Fc gamma RIIB and that was previously shown to inhibit BCR-dependent B cell activation, was required to regulate TCR- and FcR-dependent cell activation. Tyrosine 9-17 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 208-211 7761456-1 1995 Tyrosine phosphorylation of a 17-amino acid immunoreceptor tyrosine-based activation motif (ITAM), conserved in each of the signaling subunits of the T-cell antigen receptor (TCR), mediates the recruitment of ZAP-70 and syk protein-tyrosine kinases (PTKs) to the activated receptor. Tyrosine 0-8 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 175-178 7761456-1 1995 Tyrosine phosphorylation of a 17-amino acid immunoreceptor tyrosine-based activation motif (ITAM), conserved in each of the signaling subunits of the T-cell antigen receptor (TCR), mediates the recruitment of ZAP-70 and syk protein-tyrosine kinases (PTKs) to the activated receptor. Tyrosine 59-67 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 150-173 7761456-1 1995 Tyrosine phosphorylation of a 17-amino acid immunoreceptor tyrosine-based activation motif (ITAM), conserved in each of the signaling subunits of the T-cell antigen receptor (TCR), mediates the recruitment of ZAP-70 and syk protein-tyrosine kinases (PTKs) to the activated receptor. Tyrosine 59-67 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 175-178 7822334-1 1995 Signaling by the T cell antigen receptor (TCR) is mediated by 17-residue tyrosine-based activation motifs (TAM) present in the cytoplasmic tails of the TCR zeta and CD3 chains. Tyrosine 73-81 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 17-40 7852351-2 1995 By analyzing derivatives containing an activated version of either p56lck or p59fynT, it was observed that the two Src-related enzymes augmented T-cell receptor (TCR)-mediated tyrosine phosphorylation of Zap-70, as well as its association with components of the antigen receptor complex. Tyrosine 176-184 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 145-160 7852351-2 1995 By analyzing derivatives containing an activated version of either p56lck or p59fynT, it was observed that the two Src-related enzymes augmented T-cell receptor (TCR)-mediated tyrosine phosphorylation of Zap-70, as well as its association with components of the antigen receptor complex. Tyrosine 176-184 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 162-165 7852351-3 1995 Importantly, the accumulation of TCR.Zap-70 complexes quantitatively and temporally correlated with the induction of tyrosine phosphorylation of the CD3 and zeta chains of TCR. Tyrosine 117-125 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 33-36 7852351-3 1995 Importantly, the accumulation of TCR.Zap-70 complexes quantitatively and temporally correlated with the induction of tyrosine phosphorylation of the CD3 and zeta chains of TCR. Tyrosine 117-125 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 172-175 7852351-4 1995 Using a CD4-positive variant of BI-141, we also found that the ability of Zap-70 to undergo tyrosine phosphorylation and associate with TCR was enhanced by aggregation of TCR with the CD4 co-receptor. Tyrosine 92-100 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 171-174 7824949-4 1995 These results identify a specific step in the early tyrosine phosphorylation cascade that is altered after TCR engagement with modified peptide-MHC molecule complexes. Tyrosine 52-60 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 107-110 7737297-2 1995 In addition, the CD45 phosphotyrosine phosphatase (PTPase) is required for the induction of tyrosine phosphorylation by the TcR/CD3, presumably by positively regulating Src-family PTK. Tyrosine 29-37 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 124-127 7822789-1 1995 The earliest biochemical event after cross-linking of TCR is the tyrosine phosphorylation of a variety of substrates. Tyrosine 65-73 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 54-57 7822334-1 1995 Signaling by the T cell antigen receptor (TCR) is mediated by 17-residue tyrosine-based activation motifs (TAM) present in the cytoplasmic tails of the TCR zeta and CD3 chains. Tyrosine 73-81 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 42-45 7822334-1 1995 Signaling by the T cell antigen receptor (TCR) is mediated by 17-residue tyrosine-based activation motifs (TAM) present in the cytoplasmic tails of the TCR zeta and CD3 chains. Tyrosine 73-81 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 152-155 7822334-2 1995 TAMs become tyrosine-phosphorylated upon TCR stimulation, creating a high affinity binding site for the tandem SH2 domains of ZAP-70. Tyrosine 12-20 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 41-44 7822334-4 1995 Inhibition of this association prevented TCR-stimulated tyrosine phosphorylation of ZAP-70 and reduced ZAP-70 kinase activity to basal levels. Tyrosine 56-64 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 41-44 7515496-0 1994 p56lck-independent activation and tyrosine phosphorylation of p72syk by T-cell antigen receptor/CD3 stimulation. Tyrosine 34-42 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 72-95 7843239-7 1995 Both FN plus TcR-induced tyrosine phosphorylation of pp120 and degranulation are inhibited by RGD-containing peptides, implying that an RGD-dependent FN receptor is mediating phosphorylation of pp120 and enhancing TcR-mediated degranulation. Tyrosine 25-33 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 214-217 7803509-1 1994 Engagement of T-cell receptor (TcR)/CD3 complexes on T-cells rapidly provokes tyrosine phosphorylation of cellular proteins, which is thought to be an essential step to the following events of T-cell activation. Tyrosine 78-86 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 14-29 7803509-1 1994 Engagement of T-cell receptor (TcR)/CD3 complexes on T-cells rapidly provokes tyrosine phosphorylation of cellular proteins, which is thought to be an essential step to the following events of T-cell activation. Tyrosine 78-86 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 31-34 7803509-3 1994 Accumulating data suggest that p56lck is one of the kinases responsible for TcR-mediated protein tyrosine phosphorylation. Tyrosine 97-105 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 76-79 7803509-7 1994 Furthermore, injection of anti-Lck mAb inhibited TcR-mediated protein tyrosine phosphorylation of 100 kDa protein and phospholipase C gamma 1. Tyrosine 70-78 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 49-52 7526919-2 1994 In this study we examine the ability of tyrosine phosphorylated proteins from Jurkat T cells stimulated by CD2 or T cell receptor (TcR)-CD3 to interact with the src homology 2 (SH2) domains from p56lck (Lck). Tyrosine 40-48 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 114-129 7526919-2 1994 In this study we examine the ability of tyrosine phosphorylated proteins from Jurkat T cells stimulated by CD2 or T cell receptor (TcR)-CD3 to interact with the src homology 2 (SH2) domains from p56lck (Lck). Tyrosine 40-48 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 131-134 7843239-3 1995 The density of immobilized anti-CD3 or anti-TcR required to induce degranulation and tyrosine phosphorylation of cellular proteins by several cytotoxic T lymphocyte clones is quantitatively about tenfold lower in the presence of FN. Tyrosine 85-93 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 44-47 7843239-7 1995 Both FN plus TcR-induced tyrosine phosphorylation of pp120 and degranulation are inhibited by RGD-containing peptides, implying that an RGD-dependent FN receptor is mediating phosphorylation of pp120 and enhancing TcR-mediated degranulation. Tyrosine 25-33 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 13-16 7528772-1 1995 Engagement of the T cell antigen receptor (TCR) results in activation of several tyrosine kinases leading to tyrosine phosphorylation of protein substrates and activation of multiple biochemical pathways. Tyrosine 81-89 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 18-41 7528772-1 1995 Engagement of the T cell antigen receptor (TCR) results in activation of several tyrosine kinases leading to tyrosine phosphorylation of protein substrates and activation of multiple biochemical pathways. Tyrosine 81-89 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 43-46 7528772-2 1995 TCR-mediated activation of the src-family kinases, Lck and Fyn, results in tyrosine phosphorylation of the TCR zeta and CD3 chains. Tyrosine 75-83 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 0-3 7528772-2 1995 TCR-mediated activation of the src-family kinases, Lck and Fyn, results in tyrosine phosphorylation of the TCR zeta and CD3 chains. Tyrosine 75-83 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 107-110 7528772-10 1995 Similar studies demonstrated that the ZAP-70 tandem SH2 domain can bind a TCR zeta 3 TAM peptide in which both tyrosine residues are phosphorylated: Little or no binding was observed with peptides phosphorylated at only one tyrosine residue, or a nonphosphorylated peptide. Tyrosine 111-119 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 74-77 7528772-10 1995 Similar studies demonstrated that the ZAP-70 tandem SH2 domain can bind a TCR zeta 3 TAM peptide in which both tyrosine residues are phosphorylated: Little or no binding was observed with peptides phosphorylated at only one tyrosine residue, or a nonphosphorylated peptide. Tyrosine 224-232 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 74-77 7528772-14 1995 The results provide further evidence that the ZAP-70 interaction with the TCR requires prior phosphorylation of both tyrosine residues within a TAM motif. Tyrosine 117-125 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 74-77 7798261-1 1994 Stimulation of the T cell antigen receptor (TCR) leads to tyrosine phosphorylation of a number of cellular proteins, including the vav proto-oncogene product. Tyrosine 58-66 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 19-42 7798261-1 1994 Stimulation of the T cell antigen receptor (TCR) leads to tyrosine phosphorylation of a number of cellular proteins, including the vav proto-oncogene product. Tyrosine 58-66 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 44-47 7798261-5 1994 The association of the phosphoproteins with Vav-SH2 requires induction of tyrosine phosphorylation of cellular proteins since proteins from lysates of herbimycin A-treated TCR-activated T cells fail to associate with Vav-SH2. Tyrosine 74-82 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 172-175 7522245-4 1994 To approach the possibility that p56lck-mediated tyrosine phosphorylation of specific sites within TCR zeta-chain might be involved in the gp120-induced TCR signaling defect, we tested the kinase"s ability to phosphorylate various zeta peptides. Tyrosine 49-57 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 99-102 7522245-4 1994 To approach the possibility that p56lck-mediated tyrosine phosphorylation of specific sites within TCR zeta-chain might be involved in the gp120-induced TCR signaling defect, we tested the kinase"s ability to phosphorylate various zeta peptides. Tyrosine 49-57 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 153-156 7522245-6 1994 Although these data are consistent with the possibility that gp120-mediated signal disruption of TCR could be due to p56lck phosphorylation of Y84 and Y152 residues within zeta, further experiments revealed that gp120 does not induce detectable zeta tyrosine phosphorylation under conditions in which it disrupts TCR signaling. Tyrosine 250-258 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 97-100 8083187-1 1994 Tyrosine phosphorylation of multiple cellular proteins is a critical event in T cell receptor (TCR)-mediated activation. Tyrosine 0-8 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 78-93 8083187-1 1994 Tyrosine phosphorylation of multiple cellular proteins is a critical event in T cell receptor (TCR)-mediated activation. Tyrosine 0-8 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 95-98 8083187-5 1994 Here we show that the 120-kDa protein tyrosine phosphorylated in Jurkat T cells upon TCR engagement is p120cbl. Tyrosine 38-46 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 85-88 8083187-6 1994 Following stimulation through the TCR, this tyrosine phosphorylation is rapid and reversible. Tyrosine 44-52 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 34-37 7819147-0 1994 Specificity of signal transduction through CD16, TCR-CD3 and BCR receptor chains containing the tyrosine-associated activation motif. Tyrosine 96-104 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 49-52 7515496-1 1994 Activation of resting T lymphocytes by ligands to the T-cell antigen receptor (TCR)/CD3 complex is initiated by rapid tyrosine phosphorylation of cellular proteins. Tyrosine 118-126 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 54-77 7515496-1 1994 Activation of resting T lymphocytes by ligands to the T-cell antigen receptor (TCR)/CD3 complex is initiated by rapid tyrosine phosphorylation of cellular proteins. Tyrosine 118-126 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 79-82 7515496-3 1994 We show that a member of another family of PTKs, the p72syk kinase, is constitutively bound to the TCR/CD3 complex and becomes tyrosine phosphorylated and activated within 1 min after TCR/CD3 stimulation. Tyrosine 127-135 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 99-102 7515496-3 1994 We show that a member of another family of PTKs, the p72syk kinase, is constitutively bound to the TCR/CD3 complex and becomes tyrosine phosphorylated and activated within 1 min after TCR/CD3 stimulation. Tyrosine 127-135 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 184-187 8206099-2 1994 To analyze the underlying mechanism of the differential responses we compared CD3/TcR-triggered tyrosine phosphorylation in the two subsets and studied the role of co-stimulatory signals provided either by accessory cells or pharmacologic activation of protein kinase C by phorbol ester. Tyrosine 96-104 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 82-85 8206099-3 1994 Stimulation of purified CD45RA+ and CD45R0+ T cells with CD3/TcR antibodies induced similar patterns and intensities of tyrosine phosphorylation in the two subsets, but no proliferation. Tyrosine 120-128 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 61-64 7514295-8 1994 These results provide evidence for a role of the SH3 domains of Fyn and Lck in the recruitment of early tyrosine-phosphorylation substrates to the TCR-associated tyrosine kinases. Tyrosine 104-112 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 147-150 8168490-3 1994 T-cell receptor (TCR) cross-linking or coligation of the TCR with its CD4/CD8 co-receptors induced tyrosine phosphorylation and calcium signals in CD45+ but not in CD45- cells. Tyrosine 99-107 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 0-15 7514295-1 1994 Tyrosine phosphorylation of cellular proteins is the earliest identifiable event following T-cell antigen receptor (TCR) stimulation and is essential for activating downstream signaling machinery. Tyrosine 0-8 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 91-114 7514295-1 1994 Tyrosine phosphorylation of cellular proteins is the earliest identifiable event following T-cell antigen receptor (TCR) stimulation and is essential for activating downstream signaling machinery. Tyrosine 0-8 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 116-119 7514295-2 1994 Two Src-family protein-tyrosine kinases, the TCR-associated p59fyn (Fyn) and the CD4/8-associated p56lck (Lck), have emerged as the likely mediators of early tyrosine phosphorylation in T cells. Tyrosine 23-31 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 45-48 7513425-2 1994 In a T-cell receptor (TCR) signaling model, short-term pretreatment with buthionine sulfoximine, which specifically decreases intracellular glutathione, essentially abrogates the stimulation of calcium influx by anti-CD3 antibodies without significantly impairing other aspects of TCR-initiated signal transduction, such as overall levels of TCR-stimulated tyrosine phosphorylation. Tyrosine 357-365 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 22-25 8168490-3 1994 T-cell receptor (TCR) cross-linking or coligation of the TCR with its CD4/CD8 co-receptors induced tyrosine phosphorylation and calcium signals in CD45+ but not in CD45- cells. Tyrosine 99-107 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 17-20 8168490-3 1994 T-cell receptor (TCR) cross-linking or coligation of the TCR with its CD4/CD8 co-receptors induced tyrosine phosphorylation and calcium signals in CD45+ but not in CD45- cells. Tyrosine 99-107 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 57-60 8126006-2 1994 Here, we report our finding that the activation of p60fyn following TcR cross-linking results in the tyrosine phosphorylation of two Fyn-associated proteins of 82 and 116 kDa. Tyrosine 101-109 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 68-71 8020561-1 1994 Activation of resting T lymphocytes by ligands to the T cell receptor (TcR)/CD3 complex is initiated by phosphorylation of a number of key regulatory proteins on specific tyrosine residues. Tyrosine 171-179 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 54-69 7509083-2 1994 TCR stimulation induces the tyrosine phosphorylation of several cellular substrates, including the ARAMs. Tyrosine 28-36 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 0-3 8020561-1 1994 Activation of resting T lymphocytes by ligands to the T cell receptor (TcR)/CD3 complex is initiated by phosphorylation of a number of key regulatory proteins on specific tyrosine residues. Tyrosine 171-179 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 71-74 8020561-3 1994 We recently found that this enzyme is also rapidly activated following TcR/CD3 triggering and that immunoprecipitated PI3K was activated in vitro by direct tyrosine phosphorylation. Tyrosine 156-164 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 71-74 8020561-4 1994 Here we show that TcR/CD3-induced tyrosine phosphorylation and activation of PI3K in Jurkat T leukemia cells depend on the presence of the p56lck tyrosine kinase: in a variant of the Jurkat T cell line lacking p56lck, JCaM1, these responses were absent. Tyrosine 34-42 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 18-21 7693848-0 1993 The CD3 chains of the T cell antigen receptor associate with the ZAP-70 tyrosine kinase and are tyrosine phosphorylated after receptor stimulation. Tyrosine 72-80 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 22-45 8265641-6 1993 Moreover, stimulation of the gamma delta TCR results in rapid tyrosine phosphorylation of Fc epsilon RI gamma. Tyrosine 62-70 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 41-44 7693848-4 1993 A likely target of this activity is the CD3 complex itself since we observed that TCR stimulation resulted in rapid tyrosine phosphorylation of the CD3 epsilon and delta chains. Tyrosine 116-124 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 82-85 7689151-0 1993 Constitutive tyrosine phosphorylation of the T-cell receptor (TCR) zeta subunit: regulation of TCR-associated protein tyrosine kinase activity by TCR zeta. Tyrosine 13-21 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 45-60 8228621-7 1993 The observed suppression of proliferation after a long preincubation with anti-CD4 was apparently due to impairment of TcR signaling, as assessed by measurement of Ca2+ mobilization and tyrosine phosphorylation in T cells. Tyrosine 186-194 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 119-122 7689151-0 1993 Constitutive tyrosine phosphorylation of the T-cell receptor (TCR) zeta subunit: regulation of TCR-associated protein tyrosine kinase activity by TCR zeta. Tyrosine 13-21 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 62-65 7689151-0 1993 Constitutive tyrosine phosphorylation of the T-cell receptor (TCR) zeta subunit: regulation of TCR-associated protein tyrosine kinase activity by TCR zeta. Tyrosine 13-21 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 95-98 7689151-0 1993 Constitutive tyrosine phosphorylation of the T-cell receptor (TCR) zeta subunit: regulation of TCR-associated protein tyrosine kinase activity by TCR zeta. Tyrosine 13-21 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 95-98 7689151-3 1993 Approximately 35% of the tyrosine-phosphorylated TCR zeta (phospho zeta) precipitated from total cell lysates appeared to be surface associated. Tyrosine 25-33 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 49-52 7689151-5 1993 In lymph node T cells that constitutively express tyrosine-phosphorylated TCR zeta, there was a direct correlation between surface TCR-associated protein tyrosine kinase (PTK) activity and expression of phospho zeta. Tyrosine 50-58 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 74-77 7689151-5 1993 In lymph node T cells that constitutively express tyrosine-phosphorylated TCR zeta, there was a direct correlation between surface TCR-associated protein tyrosine kinase (PTK) activity and expression of phospho zeta. Tyrosine 50-58 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 131-134 7689151-7 1993 TCR ligations also contributed to the detection of several additional phosphoproteins that coprecipitated with surface TCR complexes, including a 72-kDa tyrosine-phosphorylated protein. Tyrosine 153-161 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 0-3 7689151-7 1993 TCR ligations also contributed to the detection of several additional phosphoproteins that coprecipitated with surface TCR complexes, including a 72-kDa tyrosine-phosphorylated protein. Tyrosine 153-161 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 119-122 7689151-8 1993 The presence of TCR-associated PTK activity also correlated with the binding of a 72-kDa protein, which became tyrosine phosphorylated in vitro kinase assays, to tyrosine phosphorylated TCR zeta. Tyrosine 111-119 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 16-19 7689151-8 1993 The presence of TCR-associated PTK activity also correlated with the binding of a 72-kDa protein, which became tyrosine phosphorylated in vitro kinase assays, to tyrosine phosphorylated TCR zeta. Tyrosine 111-119 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 186-189 7689151-8 1993 The presence of TCR-associated PTK activity also correlated with the binding of a 72-kDa protein, which became tyrosine phosphorylated in vitro kinase assays, to tyrosine phosphorylated TCR zeta. Tyrosine 162-170 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 16-19 7689151-8 1993 The presence of TCR-associated PTK activity also correlated with the binding of a 72-kDa protein, which became tyrosine phosphorylated in vitro kinase assays, to tyrosine phosphorylated TCR zeta. Tyrosine 162-170 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 186-189 7689151-9 1993 The cytoplasmic region of the TCR zeta chain was synthesized, tyrosine phosphorylated, and conjugated to Sepharose beads. Tyrosine 62-70 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 30-33 7689151-10 1993 Only tyrosine-phosphorylated, not nonphosphorylated, TCR zeta beads were capable of immunoprecipitating the 72-kDa protein from total cell lysates. Tyrosine 5-13 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 53-56 7689151-12 1993 These results suggest that TCR-associated PTK activity is regulated, at least in part, by the tyrosine phosphorylation status of TCR zeta. Tyrosine 94-102 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 27-30 7689151-12 1993 These results suggest that TCR-associated PTK activity is regulated, at least in part, by the tyrosine phosphorylation status of TCR zeta. Tyrosine 94-102 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 129-132 8484124-3 1993 Vav-associated GRF stimulation after TCR-CD3 ligation paralleled its tyrosine phosphorylation; both were blocked by a protein tyrosine kinase (PTK) inhibitor. Tyrosine 69-77 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 37-40 8096851-3 1993 Anti-TCR/CD3 stimulation of the TCR/CD3+ J32-3.2 cells resulted in a weak stimulation of both the phosphatidyl inositol and tyrosine kinase signal transduction pathways, as measured by changes in the level of free intracellular calcium, tyrosine phosphorylation of TCR-zeta, CD3-epsilon and ZAP-70, p56lck, or p59fyn tyrosine kinase activity and IL-2 gene activation. Tyrosine 124-132 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 5-8 8473339-3 1993 One model to explain the role of CD45 in regulating TCR signaling is that the phosphatase dephosphorylates the regulatory C-terminal tyrosine of lck. Tyrosine 133-141 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 52-55 8096851-3 1993 Anti-TCR/CD3 stimulation of the TCR/CD3+ J32-3.2 cells resulted in a weak stimulation of both the phosphatidyl inositol and tyrosine kinase signal transduction pathways, as measured by changes in the level of free intracellular calcium, tyrosine phosphorylation of TCR-zeta, CD3-epsilon and ZAP-70, p56lck, or p59fyn tyrosine kinase activity and IL-2 gene activation. Tyrosine 124-132 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 32-35 8096851-3 1993 Anti-TCR/CD3 stimulation of the TCR/CD3+ J32-3.2 cells resulted in a weak stimulation of both the phosphatidyl inositol and tyrosine kinase signal transduction pathways, as measured by changes in the level of free intracellular calcium, tyrosine phosphorylation of TCR-zeta, CD3-epsilon and ZAP-70, p56lck, or p59fyn tyrosine kinase activity and IL-2 gene activation. Tyrosine 124-132 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 32-35 1358625-2 1992 T cell activation by triggering the T cell receptor (TcR)-CD3 complex leads to a dramatic increase in tyrosine phosphorylation of multiple cellular proteins. Tyrosine 102-110 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 36-51 7681596-8 1993 Although we could not directly determine the representation of P-zeta chains among surface TCR complexes, we found that 60-70% of surface TCR complexes on immature CD4+CD8+ thymocytes were associated with tyrosine-phosphorylated protein(s) and that this percentage was inversely correlated with their signaling competence. Tyrosine 205-213 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 138-141 7681596-9 1993 These results support the concept that tyrosine phosphorylation serves as a negative regulatory modification of certain TCR-associated proteins. Tyrosine 39-47 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 120-123 1454062-1 1992 Engagement of the T cell antigen-receptor complex (TcR/CD3) induces the rapid tyrosine phosphorylation of a spectrum of substrates whose modification is crucial to the activation process. Tyrosine 78-86 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 51-54 1454062-8 1992 Stimulation of fyn activity implicates this kinase as a mediator of the tyrosine phosphorylation events originating from the TcR/CD3 complex. Tyrosine 72-80 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 125-128 1472473-1 1992 Several lines of evidence implicate a regulatory tyrosine phosphorylation in the activation of phospholipase C (PLC) by the T cell antigen receptor (TCR). Tyrosine 49-57 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 124-147 1472473-1 1992 Several lines of evidence implicate a regulatory tyrosine phosphorylation in the activation of phospholipase C (PLC) by the T cell antigen receptor (TCR). Tyrosine 49-57 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 149-152 1472473-13 1992 These findings confirm the role of a regulatory tyrosine phosphorylation in activation of PLC by the TCR. Tyrosine 48-56 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 101-104 1358625-2 1992 T cell activation by triggering the T cell receptor (TcR)-CD3 complex leads to a dramatic increase in tyrosine phosphorylation of multiple cellular proteins. Tyrosine 102-110 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 53-56 1526965-2 1992 Tyrosine phosphorylation is considered to be one of the earliest steps in TcR-mediated signal transduction. Tyrosine 0-8 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 74-77 1382975-0 1992 VCP, the mammalian homolog of cdc48, is tyrosine phosphorylated in response to T cell antigen receptor activation. Tyrosine 40-48 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 79-102 1382975-1 1992 Activation of T cells through the T cell antigen receptor (TCR) results in the rapid tyrosine phosphorylation of a number of cellular proteins, one of the earliest being a 100 kDa protein. Tyrosine 85-93 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 34-57 1382975-1 1992 Activation of T cells through the T cell antigen receptor (TCR) results in the rapid tyrosine phosphorylation of a number of cellular proteins, one of the earliest being a 100 kDa protein. Tyrosine 85-93 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 59-62 1384049-9 1992 Consistent with the formation of a multimeric CD5-TCR zeta/CD3 complex, anti-CD5 crosslinking induced tyrosine phosphorylation of numerous T-cell substrates, similar to those phosphorylated by TCR zeta/CD3 ligation. Tyrosine 102-110 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 50-53 1505025-1 1992 Signaling through the T cell antigen receptor (TCR) results both in rapid increases in tyrosine phosphorylation on a number of proteins and in the activation of the phosphatidylinositol pathway. Tyrosine 87-95 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 47-50 1380977-2 1992 In T cell variants expressing progressively lower levels of CD45 (from normal to undetectable), CD45 expression was inversely related to spontaneous tyrosine phosphorylation of multiple proteins, including the TCR zeta chain, and was directly correlated with TCR-driven phosphoinositide hydrolysis. Tyrosine 149-157 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 210-213 1505025-1 1992 Signaling through the T cell antigen receptor (TCR) results both in rapid increases in tyrosine phosphorylation on a number of proteins and in the activation of the phosphatidylinositol pathway. Tyrosine 87-95 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 22-45 1380033-2 1992 Although none of the molecules that make up the TCR possess intrinsic protein tyrosine kinase (PTK) activity, stimulation of T cells via the TCR results in the rapid appearance of newly tyrosine phosphorylated proteins in cell lysates. Tyrosine 78-86 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 141-144 1386076-5 1992 The effect induced by the GTP analogues required TCR occupancy and was independent of protein kinase C. Taken together these observations implicate a GTP-binding protein in the modulation of TCR-induced tyrosine phosphorylation. Tyrosine 203-211 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 49-52 1500851-1 1992 Stimulation of the T cell antigen receptor (TCR) activates a protein tyrosine kinase and leads to the tyrosine phosphorylation of phosphoinositide-specific phospholipase C-gamma 1 (PLC gamma 1). Tyrosine 69-77 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 44-47 1500851-3 1992 After stimulation of the TCR on Jurkat T cells, tyrosine-phosphorylated proteins of 36, 38, 58, and 63 kD coprecipitate with PLC gamma 1. Tyrosine 48-56 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 25-28 1386076-5 1992 The effect induced by the GTP analogues required TCR occupancy and was independent of protein kinase C. Taken together these observations implicate a GTP-binding protein in the modulation of TCR-induced tyrosine phosphorylation. Tyrosine 203-211 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 191-194 1533589-1 1992 Triggering of the T cell antigen receptor/CD3 (TcR/CD3) complex leads to rapid tyrosine phosphorylation of regulatory proteins that participate in initiating T cell activation and proliferation. Tyrosine 79-87 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 47-50 1534800-0 1992 A tyrosine-phosphorylated 70-kDa protein binds a photoaffinity analogue of ATP and associates with both the zeta chain and CD3 components of the activated T cell antigen receptor. Tyrosine 2-10 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 155-178 1537871-2 1992 Although the T-cell receptor for antigen (TCR) lacks intrinsic kinase activity, stimulation of this receptor induces tyrosine phosphorylation of multiple substrates. Tyrosine 117-125 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 13-40 1372649-4 1992 CD28-induced protein tyrosine phosphorylation was greatly enhanced in cells that had been preactivated by ligation of the TCR, or by pretreatment with phorbol esters. Tyrosine 21-29 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 122-125 1537871-2 1992 Although the T-cell receptor for antigen (TCR) lacks intrinsic kinase activity, stimulation of this receptor induces tyrosine phosphorylation of multiple substrates. Tyrosine 117-125 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 42-45 1537871-5 1992 Tyrosine phosphorylation induced by the TCR and the EGFR occurred on substrates unique to each receptor as well as on several shared substrates, including the zeta chain of the TCR. Tyrosine 0-8 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 40-43 1537871-5 1992 Tyrosine phosphorylation induced by the TCR and the EGFR occurred on substrates unique to each receptor as well as on several shared substrates, including the zeta chain of the TCR. Tyrosine 0-8 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 177-180 1532148-7 1992 Anti-CD4-induced tyrosine phosphorylation and activation of CD4-associated lck was also enhanced in CD45- cells, suggesting that increased lck activation compensates for the defect in CD3/TcR signaling, such that interaction of the CD3 signaling pathway with the CD4-associated pathway activates PLC even in the absence of CD45. Tyrosine 17-25 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 188-191 1371471-9 1992 By immunoprecipitation we found that TcR/CD3 stimulation induced tyrosine phosphorylation of PLC gamma 1, an effect which was enhanced by co-cross-linking CD4 to TcR/CD3. Tyrosine 65-73 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 162-165 1386038-0 1992 CD45 regulates TCR-induced signalling through tyrosine phosphorylation of phospholipase C gamma 1. Tyrosine 46-54 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 15-18 1371471-9 1992 By immunoprecipitation we found that TcR/CD3 stimulation induced tyrosine phosphorylation of PLC gamma 1, an effect which was enhanced by co-cross-linking CD4 to TcR/CD3. Tyrosine 65-73 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 37-40 1371471-0 1992 CD45 modulates T cell receptor/CD3-induced activation of human thymocytes via regulation of tyrosine phosphorylation. Tyrosine 92-100 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 15-30 1371471-4 1992 The machinery which couples the TcR/CD3 complex, CD4, and CD45 to PI-PLC appears to involve regulation of tyrosine phosphorylation, as the TcR/CD3 and CD4 receptors are associated with the tyrosine kinases p59fyn and p56lck, respectively, and CD45 has intrinsic tyrosine phosphatase activity. Tyrosine 106-114 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 32-35 1371471-4 1992 The machinery which couples the TcR/CD3 complex, CD4, and CD45 to PI-PLC appears to involve regulation of tyrosine phosphorylation, as the TcR/CD3 and CD4 receptors are associated with the tyrosine kinases p59fyn and p56lck, respectively, and CD45 has intrinsic tyrosine phosphatase activity. Tyrosine 106-114 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 139-142 2061301-0 1991 T-cell antigen receptor ligation induces tyrosine phosphorylation of phospholipase C-gamma 1. Tyrosine 41-49 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 0-23 1371471-6 1992 Co-cross-linking CD45 to the TcR/CD3 complex strongly suppressed the tyrosine phosphorylation of several intracellular substrates normally seen following TcR/CD3 stimulation. Tyrosine 69-77 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 29-32 1371471-6 1992 Co-cross-linking CD45 to the TcR/CD3 complex strongly suppressed the tyrosine phosphorylation of several intracellular substrates normally seen following TcR/CD3 stimulation. Tyrosine 69-77 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 154-157 1371471-8 1992 Since TcR/CD3 stimulation of mature T cells induces tyrosine phosphorylation of PLC gamma 1, we investigated this phenomenon in thymocytes, and asked whether ligation of CD45 might regulate this process. Tyrosine 52-60 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 6-9 2061301-8 1991 These results strongly suggest that the TCR complex expressed by Jurkat cells is functionally coupled to the phosphoinositide-dependent signaling pathway through the tyrosine phosphorylation of PLC-gamma 1. Tyrosine 166-174 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 40-43 1530920-2 1992 One of the earliest events that follows the triggering of the antigen-specific TcR-CD3 complex is a rapid tyrosine phosphorylation of several intracellular substrates, suggesting stimulation of at least one protein tyrosine kinase (PTK). Tyrosine 106-114 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 79-82 1530920-7 1992 They suggest that p59fyn is a crucial component of the TcR signal transduction machinery and that one of the earliest consequences of antigen recognition by the TcR is p59fyn-mediated phosphorylation of intracellular substrates on tyrosine residues. Tyrosine 231-239 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 161-164 1717999-1 1991 Stimulation of the T-cell antigen receptor (TCR) leads to tyrosine phosphorylation of a number of cellular proteins, including phospholipase C (PLC) gamma 1 and the TCR zeta chain. Tyrosine 58-66 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 19-42 1717999-1 1991 Stimulation of the T-cell antigen receptor (TCR) leads to tyrosine phosphorylation of a number of cellular proteins, including phospholipase C (PLC) gamma 1 and the TCR zeta chain. Tyrosine 58-66 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 44-47 1717999-4 1991 In a Jurkat cell expressing the TCR and the CD8/zeta chimeric protein, tyrosine phosphorylation and association of ZAP-70 occurs exclusively with the stimulated receptor complex. Tyrosine 71-79 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 32-35 1839225-3 1991 Recent studies suggest that the TCR-activated PTK regulates PLC activation by the phosphorylation of tyrosine residues of PLC gamma 1. Tyrosine 101-109 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 32-35 2061301-2 1991 Although the mechanism by which TCR ligation regulates PLC activity is unknown, recent studies suggest that coupling of this receptor complex to PLC activity is dependent on an intermediate protein tyrosine phosphorylation event(s). Tyrosine 198-206 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 32-35 2061301-3 1991 In the present study, we demonstrate that antibody-mediated TCR cross-linkage results in the tyrosine phosphorylation of PLC-gamma 1. Tyrosine 93-101 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 60-63 2061301-4 1991 Stimulation of the TCR for 30 s induced a 4-5-fold increase in the level of PLC activity recovered in anti-phosphotyrosine (Tyr(P)) antibody immunoprecipitates from stimulated Jurkat cells. Tyrosine 124-127 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 19-22 2061301-6 1991 Furthermore, the TCR-mediated increase in anti-Tyr(P) antibody-bound PLC activity was inhibited by staurosporine at drug concentrations identical with those required for in vivo inhibition of TCR-dependent phosphoinositide breakdown. Tyrosine 47-50 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 17-20 2061301-6 1991 Furthermore, the TCR-mediated increase in anti-Tyr(P) antibody-bound PLC activity was inhibited by staurosporine at drug concentrations identical with those required for in vivo inhibition of TCR-dependent phosphoinositide breakdown. Tyrosine 47-50 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 192-195 2061301-7 1991 Immunoblot analyses demonstrated that TCR ligation dramatically increased the level of tyrosine-phosphorylated PLC-gamma 1 present in anti-Tyr(P) antibody immunoprecipitates from stimulated Jurkat cells. Tyrosine 87-95 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 38-41 2061301-7 1991 Immunoblot analyses demonstrated that TCR ligation dramatically increased the level of tyrosine-phosphorylated PLC-gamma 1 present in anti-Tyr(P) antibody immunoprecipitates from stimulated Jurkat cells. Tyrosine 139-142 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 38-41 1829410-11 1991 Cross-linking of the TcR via plastic-coated anti-TcR mAb led to phosphorylation of CD3 gamma and epsilon and also of zeta, known to be phosphorylated on tyrosines. Tyrosine 153-162 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 21-24 1829410-11 1991 Cross-linking of the TcR via plastic-coated anti-TcR mAb led to phosphorylation of CD3 gamma and epsilon and also of zeta, known to be phosphorylated on tyrosines. Tyrosine 153-162 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 49-52 1712101-0 1991 Functional activation of the T-cell antigen receptor induces tyrosine phosphorylation of phospholipase C-gamma 1. Tyrosine 61-69 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 29-52 1712101-7 1991 Thus, the TCR functions like PTK growth factor receptors, but through an indirect interaction, to induce tyrosine phosphorylation of PLC-gamma 1. Tyrosine 105-113 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 10-13 1712101-4 1991 Western blot analysis reveals that PLC-gamma 1 is tyrosine-phosphorylated in response to TCR stimulation. Tyrosine 50-58 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 89-92 1712101-8 1991 Since other studies have implicated two members of the src family of PTKs in TCR-mediated signal transduction, our findings suggest that the induction of tyrosine phosphorylation of PLC-gamma 1 by a mechanism involving a src-like kinase may be the means by which the TCR regulates PLC activity in T cells. Tyrosine 154-162 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 77-80 1712101-6 1991 Stimulation of the TCR on mutants derived from Jurkat that are defective in TCR-induced PLC activation results in markedly reduced, if any, PLC activity recovered in phosphotyrosine immunoprecipitates and in no detectable PLC-gamma 1 tyrosine phosphorylation. Tyrosine 173-181 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 19-22 1712101-8 1991 Since other studies have implicated two members of the src family of PTKs in TCR-mediated signal transduction, our findings suggest that the induction of tyrosine phosphorylation of PLC-gamma 1 by a mechanism involving a src-like kinase may be the means by which the TCR regulates PLC activity in T cells. Tyrosine 154-162 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 267-270 1712101-6 1991 Stimulation of the TCR on mutants derived from Jurkat that are defective in TCR-induced PLC activation results in markedly reduced, if any, PLC activity recovered in phosphotyrosine immunoprecipitates and in no detectable PLC-gamma 1 tyrosine phosphorylation. Tyrosine 173-181 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 76-79 1689750-1 1990 Antiphosphotyrosine immunoblots were used to characterize tyrosine phosphorylated proteins after stimulation of the human TCR. Tyrosine 11-19 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 122-125 1708889-7 1991 In contrast, tyrosine phosphorylation of the CD3 zeta subunit but not the CD3 eta subunit follows TCR stimulation. Tyrosine 13-21 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 98-101 1708889-8 1991 Given the general importance of tyrosine phosphorylation for receptor signaling, it is likely that this difference between TCR isoforms plays a regulatory role in T-lineage function by qualitatively or quantitatively altering signaling events. Tyrosine 32-40 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 123-126 2217205-0 1990 Inhibition of tyrosine phosphorylation prevents T-cell receptor-mediated signal transduction. Tyrosine 14-22 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 48-63 2217205-3 1990 TCR activation by antigen, lectins, or anti-TCR monoclonal antibody has also been shown to cause increases in tyrosine phosphorylation of TCR-zeta and other substrates, suggesting stimulation of protein tyrosine kinase (PTK) activity. Tyrosine 110-118 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 0-3 2217205-3 1990 TCR activation by antigen, lectins, or anti-TCR monoclonal antibody has also been shown to cause increases in tyrosine phosphorylation of TCR-zeta and other substrates, suggesting stimulation of protein tyrosine kinase (PTK) activity. Tyrosine 110-118 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 44-47 2217205-3 1990 TCR activation by antigen, lectins, or anti-TCR monoclonal antibody has also been shown to cause increases in tyrosine phosphorylation of TCR-zeta and other substrates, suggesting stimulation of protein tyrosine kinase (PTK) activity. Tyrosine 110-118 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 44-47 1689750-2 1990 Increased tyrosine phosphorylation was evident on at least 12 substrates within 2 min after ligation of the TCR with mAb. Tyrosine 10-18 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 108-111 1689750-6 1990 When the TCR complex was cross-linked together with the CD4 receptor by heteroconjugate anti-CD3/CD4 mAb, an increased magnitude of tyrosine phosphorylation occurred, although no new substrates could be detected. Tyrosine 132-140 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 9-12 1689750-13 1990 Thus, these findings show that increased tyrosine phosphorylation is the earliest yet detected signal observed after ligation of the TCR complex, and furthermore suggest that tyrosine phosphorylation might link the TCR to the phosphatidylinositolbisphosphate hydrolysis signaling pathway. Tyrosine 41-49 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 133-136 1689750-13 1990 Thus, these findings show that increased tyrosine phosphorylation is the earliest yet detected signal observed after ligation of the TCR complex, and furthermore suggest that tyrosine phosphorylation might link the TCR to the phosphatidylinositolbisphosphate hydrolysis signaling pathway. Tyrosine 41-49 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 215-218 1689750-13 1990 Thus, these findings show that increased tyrosine phosphorylation is the earliest yet detected signal observed after ligation of the TCR complex, and furthermore suggest that tyrosine phosphorylation might link the TCR to the phosphatidylinositolbisphosphate hydrolysis signaling pathway. Tyrosine 175-183 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 133-136 1689750-13 1990 Thus, these findings show that increased tyrosine phosphorylation is the earliest yet detected signal observed after ligation of the TCR complex, and furthermore suggest that tyrosine phosphorylation might link the TCR to the phosphatidylinositolbisphosphate hydrolysis signaling pathway. Tyrosine 175-183 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 215-218 25990959-4 2015 The data indicated that the kinase activity of ZAP-70 stimulates negative feedback pathways that target Lck and thereby modulate the phosphorylation patterns of the immunoreceptor tyrosine-based activation motifs (ITAMs) of the CD3 and zeta chain components of the TCR and of signaling molecules downstream of Lck, including ZAP-70. Tyrosine 180-188 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 265-268 35186948-0 2021 TCR-Induced Tyrosine Phosphorylation at Tyr270 of SUMO Protease SENP1 by Lck Modulates SENP1 Enzyme Activity and Specificity. Tyrosine 12-20 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 0-3 31674657-2 2020 The non-catalytic region of tyrosine kinase (Nck) molecule is an adapter protein implicated in TCR-induced actin polymerization. Tyrosine 28-36 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 95-98 30018141-7 2018 Upstream, TCR-activated tyrosine kinases caused inducible tyrosine phosphorylation of GRK2 and were required for the GRK2-dependent events of CXCR4-Ser-339 phosphorylation and TCR-CXCR4 complex formation. Tyrosine 24-32 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 10-13 30018141-7 2018 Upstream, TCR-activated tyrosine kinases caused inducible tyrosine phosphorylation of GRK2 and were required for the GRK2-dependent events of CXCR4-Ser-339 phosphorylation and TCR-CXCR4 complex formation. Tyrosine 24-32 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 176-179 28526413-0 2017 TCR crosslinking promotes Crk adaptor protein binding to tyrosine-phosphorylated CD3zeta chain. Tyrosine 57-65 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 0-3 28465009-3 2017 A key event in the activation of TCR-coupled signaling pathways is the phosphorylation of tyrosine residues within the cytoplasmic tails of the CD3 subunits, predominantly CD3zeta. Tyrosine 90-98 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 33-36 28515365-9 2017 At the molecular level, CerS6 was required for efficient TCR signal transduction, including tyrosine phosphorylation, ZAP-70 activation, and PKCtheta/TCR colocalization. Tyrosine 92-100 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 57-60 25990959-6 2015 This model incorporated negative feedback regulation of Lck activity by the kinase activity of ZAP-70 and predicted the order in which tyrosines in the ITAMs of TCR zeta chains must be phosphorylated to be consistent with the experimental data. Tyrosine 135-144 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 161-164 25420722-2 2015 What is less clear however, is the mechanism which translates TCR engagement to the phosphorylation of immunoreceptor tyrosine-based activation motifs on CD3 chains and how this event is coupled to the delivery of Lck function. Tyrosine 118-126 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 62-65