PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 7515712-3 1994 In this study, we showed that hematopoietic cytokines, such as IL-3, IL-6, and G-CSF, all induced tyrosine-phosphorylation of Stat family proteins and Stat-associated 150-kD and 72-kD molecules in hematopoietic lineage cell lines. Tyrosine 98-106 interleukin 3 Homo sapiens 63-67 8255101-2 1993 Since interleukin 3 (IL-3) and granulocyte-macrophage colony stimulating factor (GM-CSF) induce tyrosine phosphorylation of a common set of proteins in the factor-dependent cell line M07e, we have investigated whether growth-factor-induced tyrosine phosphorylation is involved in the promotion of cell survival and suppression of apoptosis. Tyrosine 96-104 interleukin 3 Homo sapiens 6-25 8306341-9 1994 Thus, IL-3 induced tyrosine phosphorylation may result in activation of a phosphatidylcholine phospholipase C and protein kinase C. Tyrosine 19-27 interleukin 3 Homo sapiens 6-10 8255101-11 1993 These results suggest that tyrosine phosphorylation is an essential step in IL-3 and GM-CSF signal transduction. Tyrosine 27-35 interleukin 3 Homo sapiens 76-80 7691810-2 1993 In this report we demonstrate that interleukin-3, erythropoietin, and steel factor all induce the tyrosine phosphorylation of the SH2 containing protein, p52shc. Tyrosine 98-106 interleukin 3 Homo sapiens 35-48 1425916-12 1992 To the contrary, IL-3-dependent LTC4 formation appears to be regulated by serine/threonine and tyrosine phosphorylation in an antagonistic manner. Tyrosine 95-103 interleukin 3 Homo sapiens 17-21 8378773-5 1993 Instead, complexes activated by IL-3 or GM-CSF contained a tyrosine-phosphorylated protein of 80 kilodaltons. Tyrosine 59-67 interleukin 3 Homo sapiens 32-36 7682176-3 1993 GM-CSF and IL-3 induce a nearly identical pattern of protein-tyrosine phosphorylation in certain cell lines, although their receptors have no kinase domains. Tyrosine 61-69 interleukin 3 Homo sapiens 11-15 7682176-6 1993 Here we show that both GM-CSF and IL-3 induce tyrosine phosphorylation and kinase activity of the c-fps/fes proto-oncogene product (p92c-fes), a non-receptor protein-tyrosine kinase, in a human erythro-leukemia cell line, TF-1, which requires GM-CSF or IL-3 for growth. Tyrosine 46-54 interleukin 3 Homo sapiens 34-38 7682176-6 1993 Here we show that both GM-CSF and IL-3 induce tyrosine phosphorylation and kinase activity of the c-fps/fes proto-oncogene product (p92c-fes), a non-receptor protein-tyrosine kinase, in a human erythro-leukemia cell line, TF-1, which requires GM-CSF or IL-3 for growth. Tyrosine 46-54 interleukin 3 Homo sapiens 253-257 8482914-4 1993 GM-CSF, IL-3, and IL-5 induced phosphorylation of two proteins (102 and 122 kd) on tyrosine residues, as deduced from Western blot analysis with an antiphosphotyrosine monoclonal antibody (4G10). Tyrosine 83-91 interleukin 3 Homo sapiens 8-12 1382709-2 1992 GM-CSF or IL-3 stimulation of HL-60 cells pretreated with either dimethyl sulfoxide (DMSO) or retinoic acid results in increases in proliferative response as well as both tyrosine and serine phosphorylation. Tyrosine 171-179 interleukin 3 Homo sapiens 10-14 1400495-0 1992 Tyrosine phosphorylation of receptor beta subunits and common substrates in response to interleukin-3 and granulocyte-macrophage colony-stimulating factor. Tyrosine 0-8 interleukin 3 Homo sapiens 88-101 1400495-3 1992 Despite the lack of structural evidence for any enzymatic activity of the receptor polypeptides, both IL-3 and GM-CSF stimulate tyrosine phosphorylation of multiple intracellular substrates. Tyrosine 128-136 interleukin 3 Homo sapiens 102-106 1400495-6 1992 Both IL-3 and GM-CSF stimulated the phosphorylation of a similar pattern of polypeptides on tyrosine. Tyrosine 92-100 interleukin 3 Homo sapiens 5-9 1400495-7 1992 One tyrosine phosphorylated polypeptide migrated with M(r) = 135,000 and increased to 150,000 over a period of 10 min following stimulation of cells with IL-3 or GM-CSF. Tyrosine 4-12 interleukin 3 Homo sapiens 154-158 1400495-10 1992 The closely related receptor, Aic-2B, was also tyrosine phosphorylated in response to IL-3, although to a lesser extent than Aic-2A. Tyrosine 47-55 interleukin 3 Homo sapiens 86-90 1722396-1 1991 By Western blotting with anti-phosphotyrosine-specific antibody, we demonstrated that both erythropoietin (Epo) and interleukin 3 (IL3) induce rapid and transient tyrosine phosphorylation of a common set of proteins of 45, 55, 69, 87, 90, 95 and 160 KDa as a growth signal in Epo- and IL3-dependent FD-M6 cells. Tyrosine 37-45 interleukin 3 Homo sapiens 116-129 1381714-12 1992 These results indicate that SF and GM-CSF/IL-3 have partially overlapping effects on early signal transducing events, as well as striking differences, such as tyrosine phosphorylation of PLC-gamma. Tyrosine 159-167 interleukin 3 Homo sapiens 42-46 1380536-1 1992 Stimulation of hemopoietic cells with IL-3, IL-4, IL-5, granulocyte-macrophage-CSF and Steel factor-(SLF) induced tyrosine phosphorylation of a number of protein substrates. Tyrosine 114-122 interleukin 3 Homo sapiens 38-42 1380536-2 1992 Two of these proteins, designated p42 and p44, were tyrosine phosphorylated rapidly in response to treatment with IL-3, IL-5, granulocyte-macrophage-CSF and SLF, but not IL-4. Tyrosine 52-60 interleukin 3 Homo sapiens 114-118 1737930-7 1992 Immunoprecipitation of lysates from [32P]orthophosphate labeled cells with antiphosphotyrosine polyclonal antibody showed that IL-3-stimulated phosphorylation of a 70-kDa protein and a 110-kDa protein on tyrosine, and that these protein phosphorylations were completely inhibited by 30 microM genistein. Tyrosine 86-94 interleukin 3 Homo sapiens 127-131 1602836-1 1992 Unlike many other growth factor receptors, the known subunits of the receptors for the Interleukins IL-2 and IL-3 lack intrinsic tyrosine kinase activity, and yet increases in the phosphorylation of proteins on tyrosines is a rapid event in hematolymphoid cells following stimulation with these lymphokines. Tyrosine 211-220 interleukin 3 Homo sapiens 109-113 1396555-0 1992 Critical cytoplasmic domains of the common beta subunit of the human GM-CSF, IL-3 and IL-5 receptors for growth signal transduction and tyrosine phosphorylation. Tyrosine 136-144 interleukin 3 Homo sapiens 77-81 1381360-3 1992 In order to study its mechanism of action and to explore the molecular basis for its synergistic activity we have examined the proteins that become tyrosine phosphorylated in response to SF, IL-3, and GM-CSF. Tyrosine 148-156 interleukin 3 Homo sapiens 191-195 1377056-11 1992 We also showed that hIL-3 and hGM-CSF induced tyrosine phosphorylation of several proteins in CTLL transfectants, similar to those observed in human factor-dependent TF-1 cells stimulated with hIL-3 and hGM-CSF. Tyrosine 46-54 interleukin 3 Homo sapiens 20-25 1722396-1 1991 By Western blotting with anti-phosphotyrosine-specific antibody, we demonstrated that both erythropoietin (Epo) and interleukin 3 (IL3) induce rapid and transient tyrosine phosphorylation of a common set of proteins of 45, 55, 69, 87, 90, 95 and 160 KDa as a growth signal in Epo- and IL3-dependent FD-M6 cells. Tyrosine 37-45 interleukin 3 Homo sapiens 131-134 1722396-1 1991 By Western blotting with anti-phosphotyrosine-specific antibody, we demonstrated that both erythropoietin (Epo) and interleukin 3 (IL3) induce rapid and transient tyrosine phosphorylation of a common set of proteins of 45, 55, 69, 87, 90, 95 and 160 KDa as a growth signal in Epo- and IL3-dependent FD-M6 cells. Tyrosine 37-45 interleukin 3 Homo sapiens 285-288 2166606-0 1990 Signal transduction of the human granulocyte-macrophage colony-stimulating factor and interleukin-3 receptors involves tyrosine phosphorylation of a common set of cytoplasmic proteins. Tyrosine 119-127 interleukin 3 Homo sapiens 86-99 1760408-3 1991 Analysis of protein tyrosine phosphorylation showed that two common protein bands, pp95 and pp90, were phosphorylated by stimulation of either IL-2 or IL-3, suggesting the possible sharing of part of a signal transduction pathway between IL-2R and IL-3R. Tyrosine 20-28 interleukin 3 Homo sapiens 151-155 1760408-4 1991 Comparison of protein tyrosine phosphorylation profiles induced by IL-2 and IL-3 among a variety of cell lines revealed that the pp90 band is the common tyrosine phosphorylation substrate in the cell lines examined, although the general tyrosine phosphorylation pattern differed in each cell line. Tyrosine 22-30 interleukin 3 Homo sapiens 76-80 1845978-2 1991 We have previously shown that both GM-CSF and IL-3 transiently induce tyrosine phosphorylation of a number of proteins, including two cytosolic proteins, p93 and p70, which are maximally phosphorylated 5-15 min after addition of growth factor to factor-deprived cells. Tyrosine 70-78 interleukin 3 Homo sapiens 46-50 1845978-4 1991 PMA did not affect surface expression or affinity of the GM-CSF receptor but significantly inhibited GM-CSF- or IL-3-induced tyrosine phosphorylation of p93 and p70. Tyrosine 125-133 interleukin 3 Homo sapiens 112-116 1845978-7 1991 These results suggest that one aspect of GM-CSF and IL-3 signal transduction, protein tyrosine phosphorylation, can be inhibited by a mechanism which does not involve receptor down-regulation, and may involve either receptor down-regulation, and may involve either inhibition of a receptor-activated tyrosine kinase, activation of a protein tyrosine phosphatase, or both. Tyrosine 86-94 interleukin 3 Homo sapiens 52-56 1700699-0 1990 Signal transduction of human interleukin 3 and granulocyte-macrophage colony-stimulating factor through serine and tyrosine phosphorylation. Tyrosine 115-123 interleukin 3 Homo sapiens 29-95 1700699-2 1990 We found increases in tyrosine phosphorylation within 30 s of stimulation with GM-CSF or IL 3, with peak responses occurring within 2 min. Tyrosine 22-30 interleukin 3 Homo sapiens 89-93 1700699-8 1990 These data demonstrate the co-ordinate yet independent serine and tyrosine phosphorylation in IL 3- and GM-CSF-treated human myeloid cells, and thus suggest a common set of protein kinases stimulated by each separate ligand. Tyrosine 66-74 interleukin 3 Homo sapiens 94-98 2166606-5 1990 GM-CSF and IL-3 were found to induce a nearly identical pattern of protein tyrosine phosphorylation using both one- and two-dimensional gel electrophoresis. Tyrosine 75-83 interleukin 3 Homo sapiens 11-15 2166606-8 1990 Other proteins of 150, 125, 63, 55, 42, and 36 Kd were also phosphorylated on tyrosine in response to both GM-CSF and IL-3, although to a lesser degree. Tyrosine 78-86 interleukin 3 Homo sapiens 118-122 2166606-13 1990 These results suggest that tyrosine phosphorylation may be important for GM-CSF and IL-3 receptor-mediated signal transduction and that cell proliferation may be, at least partially, regulated by a balance between CSF-induced protein-tyrosine kinase activity and protein-tyrosine phosphatase activity. Tyrosine 27-35 interleukin 3 Homo sapiens 84-88 1691932-5 1990 Although both CSF-1 and IL-3 induced tyrosine phosphorylation of heterologous substrates in the dually responsive cells, differences in the patterns of substrate phosphorylation were observed in response to the two hematopoietins. Tyrosine 37-45 interleukin 3 Homo sapiens 24-28 2157296-6 1990 Interestingly, in these cells both the tyrosine phosphorylation and c-myc transcriptional pathways are still activatable following IL-3 stimulation. Tyrosine 39-47 interleukin 3 Homo sapiens 131-135 2461935-0 1988 Stimulation of factor-dependent myeloid cell lines with interleukin 3 induces tyrosine phosphorylation of several cellular substrates. Tyrosine 78-86 interleukin 3 Homo sapiens 56-69 2404337-1 1990 Interleukin-3 (IL-3) binds to its receptor with high and low affinities, induces tyrosine phosphorylation, and promotes the proliferation and differentiation of hematopoietic cells. Tyrosine 81-89 interleukin 3 Homo sapiens 0-19 2109024-3 1990 A variety of studies have implicated tyrosine phosphorylation in IL-3 signal transduction. Tyrosine 37-45 interleukin 3 Homo sapiens 65-69 2109024-5 1990 IL-3, through tyrosine phosphorylation, supports viability and growth through the regulation of transcription of a series of genes including c-myc and c-pim-1. Tyrosine 14-22 interleukin 3 Homo sapiens 0-4 2531081-2 1989 Tyrosine phosphorylation has been suggested to play an important role in IL-3-dependent cell proliferation. Tyrosine 0-8 interleukin 3 Homo sapiens 73-77 2090258-10 1990 Instead, the IL-3, GM-CSF receptors, and perhaps G-CSF appear to be coupling to kinases of both tyrosine and serine specificities. Tyrosine 96-104 interleukin 3 Homo sapiens 13-17 2789316-0 1989 Comparative analysis of IL-2 and IL-3 induced tyrosine phosphorylation. Tyrosine 46-54 interleukin 3 Homo sapiens 33-37 2789316-2 1989 We have examined the involvement of tyrosine phosphorylation in IL2 and IL3 mediated signal transduction. Tyrosine 36-44 interleukin 3 Homo sapiens 72-75 2789316-7 1989 These results suggest that signal transduction pathways for IL2 and IL3 involve tyrosine phosphorylation of protein substrates common to both lymphoid and myeloid linages. Tyrosine 80-88 interleukin 3 Homo sapiens 68-71 2656885-3 1989 Using growth factor-dependent cells, it has been shown that growth regulation by IL-3 involves binding to a high-affinity receptor of 140 Kd and activation of tyrosine phosphorylation. Tyrosine 159-167 interleukin 3 Homo sapiens 81-85 2461935-2 1988 To determine the possible signal transduction mechanisms involved in IL-3 growth regulation, we have examined the effects of IL-3 on tyrosine phosphorylation. Tyrosine 133-141 interleukin 3 Homo sapiens 125-129 2461935-3 1988 Using a monoclonal antibody against phosphotyrosine, IL-3 was found to specifically and rapidly induce tyrosine phosphorylation of cytoplasmic proteins of 70, 56, and 38 kDa and a membrane-associated glycoprotein of 140 kDa. Tyrosine 43-51 interleukin 3 Homo sapiens 53-57 2461935-8 1988 Taken together, these results support a role for tyrosine phosphorylation in the IL-3 signal transduction pathway and suggest that growth factor abrogation by oncogenes encoding tyrosine protein kinases may be due to the phosphorylation of substrates which are normally phosphorylated in response to IL-3. Tyrosine 49-57 interleukin 3 Homo sapiens 81-85 3270454-3 1988 Both early progenitor cell growth factors, such as IL-3, and late acting factors such as CSF-1, stimulate tyrosine and serine-threonine substrate phosphorylations. Tyrosine 106-114 interleukin 3 Homo sapiens 51-55 18628457-7 2008 Reintroduction of single tyrosine residues revealed the critical role of Y589 and Y591 in reconstituting interleukin-3-independent growth of FLT3-TKD-expressing cells. Tyrosine 25-33 interleukin 3 Homo sapiens 105-118 3502088-0 1987 Interleukin 3-specific tyrosine phosphorylation of a membrane glycoprotein of Mr 150,000 in multi-factor-dependent myeloid cell lines. Tyrosine 23-31 interleukin 3 Homo sapiens 0-13 3502088-1 1987 Tyrosine phosphorylation of cellular proteins induced by various hematopoietic growth factors such as interleukin 3 (IL3), granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin 4 (IL4) was studied in several multi-factor-dependent myeloid cell lines. Tyrosine 0-8 interleukin 3 Homo sapiens 102-115 3502088-1 1987 Tyrosine phosphorylation of cellular proteins induced by various hematopoietic growth factors such as interleukin 3 (IL3), granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin 4 (IL4) was studied in several multi-factor-dependent myeloid cell lines. Tyrosine 0-8 interleukin 3 Homo sapiens 117-120 3502088-2 1987 Among the growth factors, IL3 specifically induced rapid tyrosine phosphorylation of a membrane glycoprotein of mol. Tyrosine 57-65 interleukin 3 Homo sapiens 26-29 3502088-4 1987 The IL3-induced tyrosine phosphorylation of gpp150 was detected within 30 s, reached a maximum at 3 min and decreased thereafter. Tyrosine 16-24 interleukin 3 Homo sapiens 4-7 3502088-5 1987 The concentration of IL3 required for half-maximum stimulation of gpp150 tyrosine phosphorylation with 2.5 x 10(6)/ml cells was approximately 200 pM, which is the same as the dissociation constant for 125I-labeled IL3 binding. Tyrosine 73-81 interleukin 3 Homo sapiens 21-24 3502088-8 1987 The present findings suggest that tyrosine phosphorylation of gpp150 is a critical event involved in both IL3-dependent and -independent growth. Tyrosine 34-42 interleukin 3 Homo sapiens 106-109 23991218-5 2013 Additionally, we introduced a non-native tyrosine residue into our hIL-3 analog, which allowed radio-iodination to high specific activities for receptor binding studies whilst not compromising bioactivity. Tyrosine 41-49 interleukin 3 Homo sapiens 67-72 17638849-1 2007 Tyrosine and serine phosphorylation of the common beta chain (beta(c)) of the granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin-3 (IL-3), and IL-5 receptors is widely viewed as a general mechanism that provides positive inputs by coupling the receptor to signaling pathways that stimulate several cellular functions. Tyrosine 0-8 interleukin 3 Homo sapiens 152-156 17027514-3 2006 Binding of IL-3 to its cognate receptors initiates rapid tyrosine phosphorylation of Janus kinases (JAKs) and of signal transducer and activator of transcription (STAT) proteins, as well as activation of the phosphatidylinositol-3 kinase (PI-3K)/Akt and Ras/Raf/MAPK kinase (MEK)/mitogen-activated protein kinase (MAPK) pathways. Tyrosine 57-65 interleukin 3 Homo sapiens 11-15 15795318-6 2005 IL-3 treatment increases JAK2 activation and STAT5A and STAT5B tyrosine and serine phosphorylation and leads to cell cycle progression in adherent cells. Tyrosine 63-71 interleukin 3 Homo sapiens 0-4 10891441-4 2000 The Shc PTB domain, rather than the SH2 domain, associated both in vitro and in vivo with the Tyr-phosphorylated beta subunit of the IL-3 receptor and with the SH2-containing 5" inositol phosphatase (SHIP), and it recognized specific NXXpY phosphopeptides from these binding partners. Tyrosine 94-97 interleukin 3 Homo sapiens 133-137 14500382-4 2003 We found, when introduced into an interleukin 3-dependent cell line, TPR-MET induces factor independence and constitutive tyrosine phosphorylation of several cellular proteins. Tyrosine 122-130 interleukin 3 Homo sapiens 34-47 11495699-8 2001 RESULTS: STAT-5 tyrosine phosphorylation was similarly induced by PRL and IL-3, with an additive effect detected in the presence of both stimuli. Tyrosine 16-24 interleukin 3 Homo sapiens 74-78 14730712-5 2004 In this study, we found that in vitro treatment of EOC-20 microglial cells with tyrphostin AG490 blocked IL-3-induced tyrosine phosphorylation of JAK2, STAT5A, and STAT5B signaling proteins. Tyrosine 118-126 interleukin 3 Homo sapiens 105-109 14730712-6 2004 Stable transfection of EOC-20 cells with a dominant negative JAK2 mutant also blocked IL-3-induced tyrosine phosphorylation of JAK2, STAT5A, and STAT5B in microglia. Tyrosine 99-107 interleukin 3 Homo sapiens 86-90 11593427-1 2001 We have previously reported that the Jak2 tyrosine kinase but not Jak1 is tyrosine phosphorylated in the absence of IL-3 in Bcr-Abl positive M3.16 cells, which are rendered IL-3 independent by BCR-ABL gene expression. Tyrosine 42-50 interleukin 3 Homo sapiens 173-177 10993906-4 2000 Tyrosine phosphorylation of STAM2 is induced by growth factors such as epidermal growth factor and platelet-derived growth factor as well as by cytokines like IL-3. Tyrosine 0-8 interleukin 3 Homo sapiens 159-163 10704825-2 2000 p46(Shc) and p52(Shc) become heavily tyrosine phosphorylated in response to interleukin 3 (IL-3) treatment. Tyrosine 37-45 interleukin 3 Homo sapiens 76-95 10373548-8 1999 Additionally, in lymphocytes expressing SOCS-3 but not CIS, IL-2-induced tyrosine phosphorylation of STAT5b was markedly reduced, while there was only a weak effect on IL-3-mediated STAT5b tyrosine phosphorylation. Tyrosine 189-197 interleukin 3 Homo sapiens 168-172 10671297-2 2000 Tyr(462)of murine Mpl corresponds to Tyr(421)of the common beta chain of the human IL-3, IL-5 and GM-CSF receptors. Tyrosine 0-3 interleukin 3 Homo sapiens 83-87 10671297-2 2000 Tyr(462)of murine Mpl corresponds to Tyr(421)of the common beta chain of the human IL-3, IL-5 and GM-CSF receptors. Tyrosine 37-40 interleukin 3 Homo sapiens 83-87 9596995-11 1998 These data fit with the hypothesis that there is a bifurcation in the signaling pathways downstream of IL-3 receptor-induced tyrosine phosphorylation. Tyrosine 125-133 interleukin 3 Homo sapiens 103-107 10194451-5 1999 Stimulation of human hematopoietic growth factor responsive cell lines with stem cell factor (SCF), interleukin-3 (IL-3), and granulocyte-macrophage colony-stimulating factor (GM-CSF) demonstrate the rapid tyrosine phosphorylation of SHIP2 and its resulting association with SHC. Tyrosine 206-214 interleukin 3 Homo sapiens 21-48 9885438-7 1998 The results of the immunoprecipitation studies showed tyrosine phosphorylation of signal transducers and activators of transcription (Stat) 5 induced by G-CSF, GM-CSF, IL-3 and/or TPO in six cases, that of extracellular signal-regulated kinase (ERK) by GM-CSF in two cases and that of p38 by TNF in three cases. Tyrosine 54-62 interleukin 3 Homo sapiens 168-172 10378896-1 1999 Granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin (IL) -3 induced tyrosine phosphorylation of 92-kDa protein in normal human monocytes. Tyrosine 90-98 interleukin 3 Homo sapiens 62-81 8995358-1 1997 We have demonstrated that a 120-kDa protein, identified as Cbl, becomes rapidly phosphorylated on tyrosine residues following stimulation of factor-dependent cells with interleukin-3 (IL-3). Tyrosine 98-106 interleukin 3 Homo sapiens 169-182 9325013-0 1997 Lack of correlation between growth of TF-1 cells and tyrosine phosphorylation signals in response to IL-3, IL-5 and GM-CSF. Tyrosine 53-61 interleukin 3 Homo sapiens 101-105 8995358-1 1997 We have demonstrated that a 120-kDa protein, identified as Cbl, becomes rapidly phosphorylated on tyrosine residues following stimulation of factor-dependent cells with interleukin-3 (IL-3). Tyrosine 98-106 interleukin 3 Homo sapiens 184-188 8671609-3 1996 In the studies described here, we demonstrate that IL-5, IL-3 or GM-CSF stimulation induces the tyrosine phosphorylation of JAK2, and to a lesser extent JAK1, and of STAT5. Tyrosine 96-104 interleukin 3 Homo sapiens 57-61 8806691-6 1996 Our results demonstrated that in M-07e cells and in six AML cases (five IL-3 responsive and one unresponsive) p91 tyrosine phosphorylation was ligand dependent. Tyrosine 114-122 interleukin 3 Homo sapiens 72-76 8780729-1 1996 We molecularly cloned a cDNA coding for a novel phosphotyrosine molecule with a 70 kDa molecular mass, named STAM (signal transducing adaptor molecule), which is tyrosine-phosphorylated rapidly after stimulation with various cytokines such as IL-2, IL-3, IL-4, IL-7, GM-CSF, EGF and PDGF. Tyrosine 55-63 interleukin 3 Homo sapiens 249-253 8824238-7 1996 Significantly, reintroducing Tyr421 into the polyalanine-substituted mutant restored high affinity ligand binding of GM-CSF, IL-3, and IL-5, indicating that within this region the tyrosine residue alone is sufficient for high affinity ligand interaction. Tyrosine 180-188 interleukin 3 Homo sapiens 125-129 8677749-4 1996 Here we report that granulocyte/macrophage-colony- stimulating factor (GM-CSF), interleukin-3, and erythropoietin (Epo) induce rapid and transient tyrosine phosphorylation of Vav and that Vav is constitutively associated with the SH3 domain of Grb2/Ash in a human leukemia cell line UT-7. Tyrosine 147-155 interleukin 3 Homo sapiens 80-93 7545466-3 1995 Using an immunoblotting system designed to detect phosphotyrosine containing proteins and a permeabilized cell system to detect rapid changes in phosphate turnover on proteins, we show that while GM-CSF and IL-3 use tyrosine phosphorylation to mediate mitogenic signal transduction, IL-5 uses tyrosine dephosphorylation in its signaling pathway. Tyrosine 57-65 interleukin 3 Homo sapiens 207-211 7499252-5 1995 Tyrosine phosphorylation of p80 was only detected in hGM-CSF or human interleukin-3-stimulated cells, suggesting that activation of p80 might be specific for signaling via the common beta subunit. Tyrosine 0-8 interleukin 3 Homo sapiens 70-83 7545466-3 1995 Using an immunoblotting system designed to detect phosphotyrosine containing proteins and a permeabilized cell system to detect rapid changes in phosphate turnover on proteins, we show that while GM-CSF and IL-3 use tyrosine phosphorylation to mediate mitogenic signal transduction, IL-5 uses tyrosine dephosphorylation in its signaling pathway. Tyrosine 216-224 interleukin 3 Homo sapiens 207-211 7556524-3 1995 In interleukin-3 (IL-3)-dependent NFS/N1.H7 cells, tyrosine phosphorylation of FAK was not detected after stimulation with either IL-3 or Steel factor (SLF), both of which involve Ras-mediated signaling pathways. Tyrosine 51-59 interleukin 3 Homo sapiens 3-16 7556524-3 1995 In interleukin-3 (IL-3)-dependent NFS/N1.H7 cells, tyrosine phosphorylation of FAK was not detected after stimulation with either IL-3 or Steel factor (SLF), both of which involve Ras-mediated signaling pathways. Tyrosine 51-59 interleukin 3 Homo sapiens 18-22 7651724-3 1995 We found that the c-vav proto-oncogene product, Vav, is rapidly and transiently tyrosine-phosphorylated in response to erythropoietin and IL3 stimulations and that Tec kinase is also transiently activated by these cytokines. Tyrosine 80-88 interleukin 3 Homo sapiens 138-141 7545904-0 1995 TPO and IL-3 induce overlapping but distinct protein tyrosine phosphorylation in a myeloid precursor cell line. Tyrosine 53-61 interleukin 3 Homo sapiens 8-12 7545904-4 1995 The overall pattern of tyrosine phosphorylation stimulated by TPO and IL-3 in myeloid precursor cells revealed an overlapping but not identical pattern reflecting their distinct but partially redundant biological effects. Tyrosine 23-31 interleukin 3 Homo sapiens 70-74 7566975-6 1995 Of the focal adhesion proteins examined, only paxillin exhibited tyrosine phosphorylation in response to IL-3; while in cells transformed by p210BCR/ABL, paxillin, vinculin, p125FAK, talin and tensin were constitutively tyrosine phosphorylated. Tyrosine 65-73 interleukin 3 Homo sapiens 105-109 7796808-6 1995 The CIS product stably associated with the tyrosine-phosphorylated beta chain of the IL3 receptor as well as the tyrosine-phosphorylated EPO receptor. Tyrosine 43-51 interleukin 3 Homo sapiens 85-88 7543102-1 1995 The addition of interleukin-3 (IL-3) and granulocyte-macrophage colony-stimulating factor (GM-CSF) to hormone-dependent cells induces tyrosine phosphorylation of Janus protein kinase 2 (Jak2) and activates its in vitro kinase activity. Tyrosine 134-142 interleukin 3 Homo sapiens 16-29 7543102-1 1995 The addition of interleukin-3 (IL-3) and granulocyte-macrophage colony-stimulating factor (GM-CSF) to hormone-dependent cells induces tyrosine phosphorylation of Janus protein kinase 2 (Jak2) and activates its in vitro kinase activity. Tyrosine 134-142 interleukin 3 Homo sapiens 31-35 7543102-4 1995 Cross-linking of CD16/Jak2 stimulates the tyrosine phosphorylation of a large group of proteins that are also phosphorylated after the addition of IL-3 or GM-CSF and include proteins of 145, 97, 67, 52, and 42 kDa. Tyrosine 42-50 interleukin 3 Homo sapiens 147-151 7608147-5 1995 The hematopoietic STAT5-L activated by IL-3, EPO, or PRL was identified as a 97-kDa tyrosine-phosphorylated protein. Tyrosine 84-92 interleukin 3 Homo sapiens 39-43 7760829-0 1995 Cloning of murine Stat6 and human Stat6, Stat proteins that are tyrosine phosphorylated in responses to IL-4 and IL-3 but are not required for mitogenesis. Tyrosine 64-72 interleukin 3 Homo sapiens 113-117 7760829-6 1995 Using Stat6-specific antiserum, we demonstrated that Stat6 is rapidly tyrosine phosphorylated following stimulation of appropriate cell lines with IL-4 or IL-3 but is not detectably phosphorylated following stimulation with IL-2, IL-12, or erythropoietin. Tyrosine 70-78 interleukin 3 Homo sapiens 155-159 7760829-7 1995 In contrast, IL-2, IL-3, and erythropoietin induce the tyrosine phosphorylation of Stat5 while IL-12 uniquely induces the tyrosine phosphorylation of Stat4. Tyrosine 55-63 interleukin 3 Homo sapiens 19-23 7760829-7 1995 In contrast, IL-2, IL-3, and erythropoietin induce the tyrosine phosphorylation of Stat5 while IL-12 uniquely induces the tyrosine phosphorylation of Stat4. Tyrosine 122-130 interleukin 3 Homo sapiens 19-23 7534303-3 1995 Both IL-3 and v-Abl stimulated the tyrosine phosphorylation of SHC and GTPase-activating protein. Tyrosine 35-43 interleukin 3 Homo sapiens 5-9 7539516-1 1995 Diversity and similarity in signaling pathways of granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin-3 (IL-3), and stem cell factor (SCF) in five human factor-responsive leukemia cell lines were investigated by immunoblotting to detect tyrosine phosphorylation of intracellular proteins. Tyrosine 303-311 interleukin 3 Homo sapiens 171-175 7534303-4 1995 However, while IL-3 stimulated similar levels of tyrosine phosphorylation in p46shc and p52shc, v-Abl preferentially phosphorylated p52shc, an event that occurred within 1 h of temperature switch. Tyrosine 49-57 interleukin 3 Homo sapiens 15-19 7530656-0 1995 Tyrosine phosphorylation of p95Vav in myeloid cells is regulated by GM-CSF, IL-3 and steel factor and is constitutively increased by p210BCR/ABL. Tyrosine 0-8 interleukin 3 Homo sapiens 76-80 7534286-8 1995 In normal hematopoietic cells and myeloid cell lines, tyrosine phosphorylation of paxillin and coprecipitation with other cellular proteins was rapidly and transiently induced by interleukin-3 and several other hematopoietic growth factors. Tyrosine 54-62 interleukin 3 Homo sapiens 179-192 7836750-0 1995 Human IL-3 receptor signaling: rapid induction of phosphatidylcholine hydrolysis is independent of protein kinase C but dependent on tyrosine phosphorylation in transfected NIH 3T3 cells. Tyrosine 133-141 interleukin 3 Homo sapiens 6-10 7836750-12 1995 Both inhibitors effectively blocked human IL-3-induced tyrosine phosphorylation. Tyrosine 55-63 interleukin 3 Homo sapiens 42-46 7836750-14 1995 These data, combined with our previous report showing that c-jun induction by IL-3 is dependent on protein kinase C, suggest that, in hematopoietic and nonhematopoietic cells expressing the human IL-3R, phosphatidylcholine hydrolysis and protein kinase C are downstream effectors of tyrosine phosphorylation in the IL-3 signal transduction cascade resulting in immediate early response gene induction. Tyrosine 283-291 interleukin 3 Homo sapiens 78-82 7836750-14 1995 These data, combined with our previous report showing that c-jun induction by IL-3 is dependent on protein kinase C, suggest that, in hematopoietic and nonhematopoietic cells expressing the human IL-3R, phosphatidylcholine hydrolysis and protein kinase C are downstream effectors of tyrosine phosphorylation in the IL-3 signal transduction cascade resulting in immediate early response gene induction. Tyrosine 283-291 interleukin 3 Homo sapiens 196-200 7530656-4 1995 In the factor-dependent MO7e cell line, p95Vav was rapidly phosphorylated on tyrosine residues in a dose- and time-dependent manner by GM-CSF, IL-3 and Steel factor. Tyrosine 77-85 interleukin 3 Homo sapiens 143-164 7527392-2 1994 Although similar patterns of tyrosine-phosphorylated proteins characterize cells stimulated by MGF, IL-3, and GM-CSF, only the MGF receptor is a tyrosine kinase, and the heterodimeric receptors for IL-3 and GM-CSF share a common beta subunit that is devoid of enzymatic activity. Tyrosine 29-37 interleukin 3 Homo sapiens 100-104 7526158-5 1994 Tyrosine phosphorylation of Tec is not detected in the response to other cytokines controlling hematopoiesis, including colony-stimulating factor-1 (CSF-1), granulocyte-macrophage colony-stimulating factor (GM-CSF), and interleukin-3 (IL-3). Tyrosine 0-8 interleukin 3 Homo sapiens 220-233 7526158-5 1994 Tyrosine phosphorylation of Tec is not detected in the response to other cytokines controlling hematopoiesis, including colony-stimulating factor-1 (CSF-1), granulocyte-macrophage colony-stimulating factor (GM-CSF), and interleukin-3 (IL-3). Tyrosine 0-8 interleukin 3 Homo sapiens 235-239 7961995-5 1994 In an interleukin (IL)-3-dependent cell line expressing the transfected wild-type EpoR, Epo, or IL-3 induced tyrosine phosphorylation of Shc and its association with Grb2. Tyrosine 109-117 interleukin 3 Homo sapiens 6-24 7961995-5 1994 In an interleukin (IL)-3-dependent cell line expressing the transfected wild-type EpoR, Epo, or IL-3 induced tyrosine phosphorylation of Shc and its association with Grb2. Tyrosine 109-117 interleukin 3 Homo sapiens 96-100 7522233-0 1994 Interleukin (IL)-3 and granulocyte/macrophage colony-stimulating factor, but not IL-4, induce tyrosine phosphorylation, activation, and association of SHPTP2 with Grb2 and phosphatidylinositol 3"-kinase. Tyrosine 94-102 interleukin 3 Homo sapiens 0-18 7522233-1 1994 Binding of interleukin (IL)-3 and granulocyte/macrophage colony-stimulating factor (GM-CSF) to their high affinity cell surface receptors induces tyrosine phosphorylation of a similar set of protein substrates. Tyrosine 146-154 interleukin 3 Homo sapiens 11-29 7522233-3 1994 The Src homology 2 (SH2) domain of the adaptor protein Grb2 bound with high affinity to tyrosine-phosphorylated SHPTP2 following treatment of cells with IL-3 or GM-CSF, but not IL-4. Tyrosine 88-96 interleukin 3 Homo sapiens 153-157