PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 2468666-0 1989 Thrombin and collagen induce rapid phosphorylation of a common set of cellular proteins on tyrosine in human platelets. Tyrosine 91-99 coagulation factor II, thrombin Homo sapiens 0-8 2468666-1 1989 The ability of thrombin and collagen to induce protein-tyrosine phosphorylation in intact human platelets was assessed by using antibodies to phosphotyrosine in conjugation with immunoblots. Tyrosine 55-63 coagulation factor II, thrombin Homo sapiens 15-23 2468666-2 1989 Upon stimulation by thrombin there was an increase in the amount of protein-tyrosine phosphorylation of three bands with molecular masses of 135, 124, and 76 kDa in a time-dependent manner. Tyrosine 76-84 coagulation factor II, thrombin Homo sapiens 20-28 2468666-6 1989 The time courses for thrombin- or collagen-induced protein-tyrosine phosphorylation were similar to that for [14C] serotonin release. Tyrosine 59-67 coagulation factor II, thrombin Homo sapiens 21-29 2464741-0 1988 Platelet tyrosine-specific protein phosphorylation is regulated by thrombin. Tyrosine 9-17 coagulation factor II, thrombin Homo sapiens 67-75 2464830-0 1989 Thrombin treatment induces rapid changes in tyrosine phosphorylation in platelets. Tyrosine 44-52 coagulation factor II, thrombin Homo sapiens 0-8 2464741-3 1988 Immunoblotting with antiphosphotyrosine antiserum showed that thrombin caused dramatic changes in the tyrosine phosphorylation of a number of individual protein bands and that these changes occurred in three distinct temporal waves. Tyrosine 31-39 coagulation factor II, thrombin Homo sapiens 62-70 2464741-4 1988 Most but not all of the protein bands phosphorylated at tyrosine in response to thrombin were also tyrosine phosphorylated in response to chilling or the combination of ionophore A23187 and tetradecanoylphorbol acetate. Tyrosine 56-64 coagulation factor II, thrombin Homo sapiens 80-88 2464741-4 1988 Most but not all of the protein bands phosphorylated at tyrosine in response to thrombin were also tyrosine phosphorylated in response to chilling or the combination of ionophore A23187 and tetradecanoylphorbol acetate. Tyrosine 99-107 coagulation factor II, thrombin Homo sapiens 80-88 2464741-5 1988 Thrombin stimulated the phosphorylation of the tyrosine kinase pp60c-src, primarily at Ser-12 and Tyr-527, although the effects of these phosphorylations on platelet pp60c-src function were not apparent. Tyrosine 98-101 coagulation factor II, thrombin Homo sapiens 0-8 3016716-10 1986 The peptide Tyr-His-His-Lys-Arg-Lys-Arg-Lys-Gln-Arg-Gly-Asp-Val was labeled with 125I to quantitate its binding to thrombin-stimulated platelets; at saturation, 59,990 molecules were bound per cell (Kd = 3.8 X 10(-7) M). Tyrosine 12-15 coagulation factor II, thrombin Homo sapiens 115-123 2847349-5 1988 Oxidation of tryptophan residues with N-bromosuccinimide or iodination of tyrosine residues of thrombin led to reduced clotting and amidolytic activity as well as a reduced ability to interact with thrombomodulin. Tyrosine 74-82 coagulation factor II, thrombin Homo sapiens 95-103 2847349-9 1988 These results indicate that one or more tryptophan, arginine and tyrosine residues are essential for the recognition of thrombin by thrombomodulin whilst the carbohydrate side chain and the active site residues of the thrombin molecule are not involved in thrombomodulin binding. Tyrosine 65-73 coagulation factor II, thrombin Homo sapiens 120-128 2847349-9 1988 These results indicate that one or more tryptophan, arginine and tyrosine residues are essential for the recognition of thrombin by thrombomodulin whilst the carbohydrate side chain and the active site residues of the thrombin molecule are not involved in thrombomodulin binding. Tyrosine 65-73 coagulation factor II, thrombin Homo sapiens 218-226 3771562-9 1986 The amino acid sequence of this peptide was Ala-Ile-Glu-Gly-Cys-Thr-Ala-Thr-Ser-Glu-Tyr-Gln-Thr-Phe-Phe-Asn-Pro-Arg, corresponding to residues 269-287 in prothrombin except for the substitution of cysteine for arginine at residue 273. Tyrosine 84-87 coagulation factor II, thrombin Homo sapiens 154-165 7126590-8 1982 The modification of tyrosine and tryptophan residues in thrombin influenced the clotting activity, while these were not involved for esterolysis. Tyrosine 20-28 coagulation factor II, thrombin Homo sapiens 56-64 32446352-5 2020 Interestingly, inhibition of CD73 in thrombin-treated platelets led to enhanced tyrosine phosphorylation of proteins and rise in intracellular free calcium, [Ca2+]i, thus signifying the inhibitory role of the ectonucleotidase on agonist-mediated platelet signaling. Tyrosine 80-88 coagulation factor II, thrombin Homo sapiens 37-45 7306521-6 1981 Solvent perturbation studies of alpha-thrombin and gamma=thrombin inhibited by phenyl-methanesulfonyl fluoride showed that 0.3 +/- tryptophan and 0.9 +/- 0.3 tyrosine residues in alpha-thrombin and 0.6 +/- 0.3 tryptophan and 1.3 +/- 0.4 tyrosine residues in gamma-thrombin were blocked by the inhibitor. Tyrosine 158-166 coagulation factor II, thrombin Homo sapiens 57-65 7306521-6 1981 Solvent perturbation studies of alpha-thrombin and gamma=thrombin inhibited by phenyl-methanesulfonyl fluoride showed that 0.3 +/- tryptophan and 0.9 +/- 0.3 tyrosine residues in alpha-thrombin and 0.6 +/- 0.3 tryptophan and 1.3 +/- 0.4 tyrosine residues in gamma-thrombin were blocked by the inhibitor. Tyrosine 158-166 coagulation factor II, thrombin Homo sapiens 57-65 7306521-6 1981 Solvent perturbation studies of alpha-thrombin and gamma=thrombin inhibited by phenyl-methanesulfonyl fluoride showed that 0.3 +/- tryptophan and 0.9 +/- 0.3 tyrosine residues in alpha-thrombin and 0.6 +/- 0.3 tryptophan and 1.3 +/- 0.4 tyrosine residues in gamma-thrombin were blocked by the inhibitor. Tyrosine 158-166 coagulation factor II, thrombin Homo sapiens 57-65 7306521-6 1981 Solvent perturbation studies of alpha-thrombin and gamma=thrombin inhibited by phenyl-methanesulfonyl fluoride showed that 0.3 +/- tryptophan and 0.9 +/- 0.3 tyrosine residues in alpha-thrombin and 0.6 +/- 0.3 tryptophan and 1.3 +/- 0.4 tyrosine residues in gamma-thrombin were blocked by the inhibitor. Tyrosine 237-245 coagulation factor II, thrombin Homo sapiens 57-65 7306521-6 1981 Solvent perturbation studies of alpha-thrombin and gamma=thrombin inhibited by phenyl-methanesulfonyl fluoride showed that 0.3 +/- tryptophan and 0.9 +/- 0.3 tyrosine residues in alpha-thrombin and 0.6 +/- 0.3 tryptophan and 1.3 +/- 0.4 tyrosine residues in gamma-thrombin were blocked by the inhibitor. Tyrosine 237-245 coagulation factor II, thrombin Homo sapiens 57-65 7306521-6 1981 Solvent perturbation studies of alpha-thrombin and gamma=thrombin inhibited by phenyl-methanesulfonyl fluoride showed that 0.3 +/- tryptophan and 0.9 +/- 0.3 tyrosine residues in alpha-thrombin and 0.6 +/- 0.3 tryptophan and 1.3 +/- 0.4 tyrosine residues in gamma-thrombin were blocked by the inhibitor. Tyrosine 237-245 coagulation factor II, thrombin Homo sapiens 57-65 7306521-7 1981 These subtle differences in the extent of blocking of tyrosine and tryptophan suggest a tighter conformation in the catalytic site of gamma-thrombin compared to that of alpha-thrombin. Tyrosine 54-62 coagulation factor II, thrombin Homo sapiens 140-148 7306521-7 1981 These subtle differences in the extent of blocking of tyrosine and tryptophan suggest a tighter conformation in the catalytic site of gamma-thrombin compared to that of alpha-thrombin. Tyrosine 54-62 coagulation factor II, thrombin Homo sapiens 175-183 33369157-11 2021 CONCLUSION: Residues 346-349 with sulfated Tyr provided a thrombin-interactive site responsible for activation and cleavage at Arg372 . Tyrosine 43-46 coagulation factor II, thrombin Homo sapiens 58-66 5534304-0 1970 Tyrosine and tryptophan residues and amino groups in thrombin related to enzymic activities. Tyrosine 0-8 coagulation factor II, thrombin Homo sapiens 53-61 22956765-7 2012 Moreover, on thrombin challenge, we find prolonged elevation in tyrosine phosphorylation levels of VE-cadherin-associated beta-catenin in SHP2-depleted cells. Tyrosine 64-72 coagulation factor II, thrombin Homo sapiens 13-21 27435675-4 2016 The closed conformation predominates (70%) and features an unanticipated intramolecular collapse of Tyr(93) in kringle-1 onto Trp(547) in the protease domain that obliterates access to the active site and protects the zymogen from autoproteolytic conversion to thrombin. Tyrosine 100-103 coagulation factor II, thrombin Homo sapiens 261-269 26090670-6 2015 We also found that Ang-1 inhibits thrombin induced tyrosine phosphorylation of Occludin and promote Occludin interaction with Zona Occludens-1 (ZO-1) to stabilize TJs. Tyrosine 51-59 coagulation factor II, thrombin Homo sapiens 34-42 25201728-1 2014 Sulfated beta-O4 lignin (SbO4L), a non-saccharide glycosaminoglycan mimetic, was recently disclosed as a novel exosite 2-directed thrombin inhibitor with the capability of mimicking sulfated tyrosine sequences of glycoprotein Ibalpha resulting in dual anticoagulant and antiplatelet activities. Tyrosine 191-199 coagulation factor II, thrombin Homo sapiens 130-138 23488775-0 2013 Therapeutic implications of endothelin and thrombin G-protein-coupled receptor transactivation of tyrosine and serine/threonine kinase cell surface receptors. Tyrosine 98-106 coagulation factor II, thrombin Homo sapiens 43-51 32327488-7 2020 Inhibition of PLD2 activity resulted in prolonged phosphorylation of Tyr-658 in VE-cadherin during the recovery phase 3h post-thrombin challenge. Tyrosine 69-72 coagulation factor II, thrombin Homo sapiens 126-134 31221752-3 2019 Here a bioinformatics approach is used to identify seven tick proteins with putative thrombin inhibitory activity that we predict to be posttranslationally sulfated at two conserved tyrosine residues. Tyrosine 182-190 coagulation factor II, thrombin Homo sapiens 85-93 31221752-5 2019 Downstream functional characterization validated the thrombin-directed activity of all target molecules and revealed that posttranslational sulfation of specific tyrosine residues crucially modulates potency. Tyrosine 162-170 coagulation factor II, thrombin Homo sapiens 53-61 28445805-9 2017 These findings demonstrate, for the first time, that thrombin stimulation of RPE cell transformation and migration are regulated by FAK tyrosine phosphorylation. Tyrosine 136-144 coagulation factor II, thrombin Homo sapiens 53-61 25967238-2 2015 In the present study, we show that the time-dependent protein-tyrosine phosphorylation triggered by thrombin in human or murine platelets was totally suppressed only upon concomitant chelation of intracellular Ca(2+) and inhibition of SFKs. Tyrosine 62-70 coagulation factor II, thrombin Homo sapiens 100-108 24790104-5 2014 In addition, thrombin treatment led to increases in phospho-focal adhesion kinase (tyrosine 397), ERK1/2 phosphorylation and cell proliferation, which were significantly inhibited in cells treated with beta1 integrin antibody or Rap1A siRNA. Tyrosine 83-91 coagulation factor II, thrombin Homo sapiens 13-21 24025335-3 2013 Thrombin induced tyrosine phosphorylation of Pyk2, Gab1, and p115 RhoGEF, leading to Rac1- and RhoA-dependent Pak2 activation. Tyrosine 17-25 coagulation factor II, thrombin Homo sapiens 0-8 22236469-5 2012 The compound also inhibited thrombin-evoked protein-Tyr-phosphorylation. Tyrosine 52-55 coagulation factor II, thrombin Homo sapiens 28-36 18768213-4 2009 We showed that thrombin transiently induced tyrosine- phosphorylation of EphA2 in a Src-kinase dependent manner. Tyrosine 44-52 coagulation factor II, thrombin Homo sapiens 15-23 21388249-8 2011 Protein tyrosine phosphorylation by both thrombin and collagen was reduced by adrenaline, and inhibitors of autocrine stimulation counteracted this reduction. Tyrosine 8-16 coagulation factor II, thrombin Homo sapiens 41-49 20600849-5 2010 In PAR1- and PAR4-desensitized platelets, thrombin also induced tyrosine phosphorylation of some substrates, but, surprisingly, this event was largely independent of GPIbalpha binding, as it persisted upon platelet treatment with mocarhagin. Tyrosine 64-72 coagulation factor II, thrombin Homo sapiens 42-50 19718473-3 2009 In this study, we demonstrate that the scaffolding and F-actin binding protein LASP-1 undergoes tyrosine phosphorylation in thrombin-stimulated human platelets. Tyrosine 96-104 coagulation factor II, thrombin Homo sapiens 124-132 19359598-10 2009 Inhibition of thrombin signaling by recombinant hirudin also blocked balloon injury-induced EGFR tyrosine phosphorylation and PAK1 activity. Tyrosine 97-105 coagulation factor II, thrombin Homo sapiens 14-22 22950787-9 2012 Sitagliptin decreased intracellular free calcium (2.5-fold) and tyrosine phosphorylation of multiple proteins in thrombin-induced platelet activation. Tyrosine 64-72 coagulation factor II, thrombin Homo sapiens 113-121 20184328-10 2010 The results further suggest that thrombin is less tolerant than fXa in accommodating the nonoptimal P1 Tyr of the AT mutant in its active-site pocket. Tyrosine 103-106 coagulation factor II, thrombin Homo sapiens 33-41 19660790-9 2009 In resting or in thrombin stimulated platelets of patients total protein tyrosine phosphorylation, p38MAPK and cytosolic phospholipase A(2) phosphorylation were increased. Tyrosine 73-81 coagulation factor II, thrombin Homo sapiens 17-25 17854313-5 2007 Thrombin and stirring also induced WASP tyrosine phosphorylation, a rapid activating reaction, and proteolytic inactivation by cysteine protease calpain. Tyrosine 40-48 coagulation factor II, thrombin Homo sapiens 0-8 18362147-10 2008 Thus, these data unveil a novel pathway in which Syk signals downstream of PKC-delta to mediate thrombin induced ICAM-1 expression in endothelial cells by increasing transcriptional capacity of NF-kappaB via a mechanism that relies on tyrosine phosphorylation of RelA/p65. Tyrosine 235-243 coagulation factor II, thrombin Homo sapiens 96-104 19030108-3 2008 PKCdelta is robustly expressed in human platelets and undergoes transient tyrosine phosphorylation upon stimulation by thrombin or the collagen receptor, GPVI, which becomes sustained in the presence of the pan-PKC inhibitor, Ro 31-8220. Tyrosine 74-82 coagulation factor II, thrombin Homo sapiens 119-127 18362147-0 2008 Activation of Syk by protein kinase C-delta regulates thrombin-induced intercellular adhesion molecule-1 expression in endothelial cells via tyrosine phosphorylation of RelA/p65. Tyrosine 141-149 coagulation factor II, thrombin Homo sapiens 54-62 18362147-4 2008 Stimulation of human vascular endothelial cells with thrombin resulted in a time-dependent phosphorylation of Syk on tyrosine 525 and 526, an indication of Syk activation. Tyrosine 117-125 coagulation factor II, thrombin Homo sapiens 53-61 17904203-5 2008 RESULTS: Inhibition of tyrosine phosphorylation blocked shape change and cytoskeletal assembly induced by collagen, and inhibited, though partially, those effects due to thrombin. Tyrosine 23-31 coagulation factor II, thrombin Homo sapiens 170-178 17570831-2 2007 It is phosphorylated on tyrosine residues in response to thrombin and collagen, and it has recently been shown that Tyr311 is phosphorylated in response to PAR (protease-activated receptor) 1 and PAR4 receptor activation. Tyrosine 24-32 coagulation factor II, thrombin Homo sapiens 57-65 17721620-0 2007 Tyrosine phosphorylation / dephosphorylation balance is involved in thrombin-evoked microtubular reorganisation in human platelets. Tyrosine 0-8 coagulation factor II, thrombin Homo sapiens 68-76 17601492-4 2007 The beta-nitrostyrene derivatives inhibited thrombin- or collagen-induced human platelet aggregation, ATP secretion, GPIIb/IIIa activation and protein tyrosine phosphorylation. Tyrosine 151-159 coagulation factor II, thrombin Homo sapiens 44-52 17721620-10 2007 These findings indicate that thrombin induces microtubular remodelling by modifying the balance between protein tyrosine phosphorylation and dephosphorylation. Tyrosine 112-120 coagulation factor II, thrombin Homo sapiens 29-37 17012367-2 2007 We now provide evidence that activation of c-Src is crucial in signaling thrombin-induced ICAM-1 expression via tyrosine phosphorylation of RelA/p65. Tyrosine 112-120 coagulation factor II, thrombin Homo sapiens 73-81 17461933-2 2007 Earlier studies demonstrated that platelet activation by thrombin results in tyrosine phosphorylation of PMCA, which inhibits pump activity. Tyrosine 77-85 coagulation factor II, thrombin Homo sapiens 57-65 17461933-6 2007 RESULTS: Western blot analysis of immunoprecipitates showed that introduction of the inhibitory peptide decreased tyrosine phosphorylation of PMCA by nearly 60% in saponin-permeabilized, thrombin-treated platelets as compared with the scrambled control peptide. Tyrosine 114-122 coagulation factor II, thrombin Homo sapiens 187-195 17461933-7 2007 Concomitant with inhibition of PMCA tyrosine phosphorylation was a significant decrease in [Ca(2+)](i) during thrombin-mediated platelet activation. Tyrosine 36-44 coagulation factor II, thrombin Homo sapiens 110-118 17461933-8 2007 The functional consequence of reduced PMCA tyrosine phosphorylation and decreased [Ca(2+)](i) was a significant delay in the onset of thrombin-mediated platelet aggregation. Tyrosine 43-51 coagulation factor II, thrombin Homo sapiens 134-142 17012367-8 2007 We also observed that thrombin induced tyrosine phosphorylation of RelA/p65, and this phosphorylation was lost upon inhibition of c-Src, consistent with the requirement of activated c-Src for interaction with RelA/p65. Tyrosine 39-47 coagulation factor II, thrombin Homo sapiens 22-30 16169188-6 2006 In addition, thrombin- or U46619-induced tyrosine phosphorylation of several substrates typically involved in FcgammaRIIA-mediated signalling, such as Syk and PLCgamma2, was clearly reduced by incubation with anti-FcgammaRIIA antibody IV.3. Tyrosine 41-49 coagulation factor II, thrombin Homo sapiens 13-21 16920985-6 2006 Thrombin caused time-dependent increases in phosphorylation of c-Src at tyrosine 416 and c-Src activity. Tyrosine 72-80 coagulation factor II, thrombin Homo sapiens 0-8 17076509-6 2006 A molecule containing two tyrosine residues separated by a linker that matched the spacing on the beta-sheet scaffold inhibited thrombin, whereas a similar dityrosine molecule separated by a shorter 6 A linker could not. Tyrosine 26-34 coagulation factor II, thrombin Homo sapiens 128-136 17001711-1 2006 In the completion of our fluorine scan of tricyclic inhibitors to map the fluorophilicity/fluorophobicity of the thrombin active site, a series of 11 new ligands featuring alkyl, alkenyl, and fluoroalkyl groups was prepared to explore fluorine effects on binding into the hydrophobic proximal (P) pocket, lined by Tyr 60A and Trp 60D, His 57, and Leu 99. Tyrosine 314-317 coagulation factor II, thrombin Homo sapiens 113-121 16898954-8 2006 In vitro tyrosine dephosphorylation of platelet eNOS using a recombinant protein tyrosine phosphatase enhanced thrombin-induced activity compared to thrombin alone, but had no effect on endothelial eNOS activity either at basal or after stimulation with bradykinin. Tyrosine 9-17 coagulation factor II, thrombin Homo sapiens 111-119 16898954-8 2006 In vitro tyrosine dephosphorylation of platelet eNOS using a recombinant protein tyrosine phosphatase enhanced thrombin-induced activity compared to thrombin alone, but had no effect on endothelial eNOS activity either at basal or after stimulation with bradykinin. Tyrosine 9-17 coagulation factor II, thrombin Homo sapiens 149-157 16169188-7 2006 Upon stimulation with thrombin, FcgammaRIIA relocated in lipid rafts, and thrombin-induced tyrosine phosphorylation of FcgammaRIIA occurred within these membrane domains. Tyrosine 91-99 coagulation factor II, thrombin Homo sapiens 22-30 16169188-7 2006 Upon stimulation with thrombin, FcgammaRIIA relocated in lipid rafts, and thrombin-induced tyrosine phosphorylation of FcgammaRIIA occurred within these membrane domains. Tyrosine 91-99 coagulation factor II, thrombin Homo sapiens 74-82 16527988-3 2006 Thrombin activated STAT-5 as measured by its tyrosine phosphorylation, DNA binding, and reporter gene activity. Tyrosine 45-53 coagulation factor II, thrombin Homo sapiens 0-8 16581796-3 2006 Here, we show that AP2, a clathrin adaptor, binds directly to a tyrosine-based motif in the cytoplasmic tail of PAR1 and is essential for constitutive receptor internalization and cellular recovery of thrombin signaling. Tyrosine 64-72 coagulation factor II, thrombin Homo sapiens 201-209 16126727-8 2005 The mutant thrombin evoked a Ca2+ spark and progressive phosphorylation of Src family kinases at tyrosine 416 and NOS3 only at threonine 495. Tyrosine 97-105 coagulation factor II, thrombin Homo sapiens 11-19 16148030-8 2006 In addition, thrombin stimulated the tyrosine phosphorylation of EGF receptor (EGFR), and inhibition of its kinase activity significantly blocked Akt and S6K1 phosphorylation, Fra-1 and FGF-2 expression, DNA synthesis, and motility induced by thrombin in VSMC. Tyrosine 37-45 coagulation factor II, thrombin Homo sapiens 13-21 16287612-0 2005 Efficient tyrosine phosphorylation of proteins after activation of platelets with thrombin depends on intact glycoprotein Ib. Tyrosine 10-18 coagulation factor II, thrombin Homo sapiens 82-90 16287612-3 2005 Tyrosine phosphorylation in whole platelet lysates and in cytoskeletal extracts was evaluated after activation with thrombin and with the thrombin receptor-activating peptide (TRAP). Tyrosine 0-8 coagulation factor II, thrombin Homo sapiens 116-124 16287612-5 2005 After activation of control platelets with thrombin or TRAP, multiple proteins became tyrosine phosphorylated in platelet lysates and some of them associated with the cytoskeletal fraction. Tyrosine 86-94 coagulation factor II, thrombin Homo sapiens 43-51 16287612-10 2005 The signaling mechanisms occurring through tyrosine phosphorylation of proteins triggered by thrombin seem to be dependent on intact GPIb. Tyrosine 43-51 coagulation factor II, thrombin Homo sapiens 93-101 16001397-0 2005 Detection of the centriole tyr- or acet-tubulin changes in endothelial cells treated with thrombin using microscopic immunocytochemistry. Tyrosine 27-30 coagulation factor II, thrombin Homo sapiens 90-98 15016733-7 2004 Thrombin stimulated GIT1 tyrosine phosphorylation with a time course similar to FAK phosphorylation in a Rho kinase- and Src-dependent manner. Tyrosine 25-33 coagulation factor II, thrombin Homo sapiens 0-8 15811957-1 2005 Thrombin has been known to cause tyrosine phosphorylation of protein kinase C delta (PKCdelta) in platelets, but the molecular mechanisms and function of this tyrosine phosphorylation is not known. Tyrosine 33-41 coagulation factor II, thrombin Homo sapiens 0-8 15811957-1 2005 Thrombin has been known to cause tyrosine phosphorylation of protein kinase C delta (PKCdelta) in platelets, but the molecular mechanisms and function of this tyrosine phosphorylation is not known. Tyrosine 159-167 coagulation factor II, thrombin Homo sapiens 0-8 15811957-8 2005 We conclude that thrombin causes tyrosine phosphorylation of PKCdelta in a calcium- and Src-family kinase-dependent manner in platelets, with functional implications in thromboxane A2 generation. Tyrosine 33-41 coagulation factor II, thrombin Homo sapiens 17-25 15795233-9 2005 All together these results indicate that HS1 Tyr phosphorylation catalyzed by Syk and Lyn plays a crucial role in the translocation of the protein to the membrane and is involved in the cytoskeleton rearrangement triggered by thrombin in human platelets. Tyrosine 45-48 coagulation factor II, thrombin Homo sapiens 226-234 15886804-0 2005 P2Y12 ADP receptor-dependent tyrosine phosphorylation of proteins of 27 and 31 kDa in thrombin-stimulated human platelets. Tyrosine 29-37 coagulation factor II, thrombin Homo sapiens 86-94 15886804-1 2005 In thrombin-stimulated human platelets several proteins undergo rapid and transient changes in tyrosine phosphorylation. Tyrosine 95-103 coagulation factor II, thrombin Homo sapiens 3-11 15886804-8 2005 Furthermore, epinephrine, acting via inhibitory Gz-coupled alpha(2A)-adrenoceptors, bypasses the inhibitory effect of AR-C69931MX on thrombin-induced p27 and p31 tyrosine phosphorylation. Tyrosine 162-170 coagulation factor II, thrombin Homo sapiens 133-141 15886804-10 2005 Elevating cAMP levels with PGI(2) or forskolin precludes thrombin-induced p27 and p31 tyrosine phosphorylation. Tyrosine 86-94 coagulation factor II, thrombin Homo sapiens 57-65 15582990-4 2005 The unfavorable contributions to binding disappear and the number of residues promoting the thrombin-protein C interaction is reduced to Tyr-60a and Asp-189. Tyrosine 137-140 coagulation factor II, thrombin Homo sapiens 92-100 15667216-9 2005 Backbone dynamics of the thrombin-binding residues Tyr 413 and Ile 414 are inversely correlated with thrombin binding. Tyrosine 51-54 coagulation factor II, thrombin Homo sapiens 25-33 15667216-9 2005 Backbone dynamics of the thrombin-binding residues Tyr 413 and Ile 414 are inversely correlated with thrombin binding. Tyrosine 51-54 coagulation factor II, thrombin Homo sapiens 101-109 15667216-10 2005 The preordering of the backbone of Tyr 413 and Ile 414 only occurs in the two-domain fragments, revealing a role for the fourth domain in thrombin binding as well as in anticoagulant cofactor activity. Tyrosine 35-38 coagulation factor II, thrombin Homo sapiens 138-146 16001397-3 2005 A decrease in the intensity of centriolar staining for tyr-tubulin was detected in HUVEC within 1 min after thrombin addition, while in HAEC centriolar staining for tyr-tubulin became less intense only 5 min later. Tyrosine 55-58 coagulation factor II, thrombin Homo sapiens 108-116 15811957-0 2005 Molecular mechanism and functional implications of thrombin-mediated tyrosine phosphorylation of PKCdelta in platelets. Tyrosine 69-77 coagulation factor II, thrombin Homo sapiens 51-59 15795233-0 2005 Thrombin-induced tyrosine phosphorylation of HS1 in human platelets is sequentially catalyzed by Syk and Lyn tyrosine kinases and associated with the cellular migration of the protein. Tyrosine 17-25 coagulation factor II, thrombin Homo sapiens 0-8 15795233-1 2005 Thrombin stimulation of platelets triggers Tyr phosphorylation of several signaling proteins, most of which remain unidentified. Tyrosine 43-46 coagulation factor II, thrombin Homo sapiens 0-8 15795233-2 2005 In this study, we demonstrate for the first time that hematopoietic lineage cell-specific protein 1 (HS1) undergoes a transient Tyr phosphorylation in human platelets stimulated with thrombin. Tyrosine 128-131 coagulation factor II, thrombin Homo sapiens 183-191 15968387-4 2005 To further investigate the role of this loop, a mutant of thrombin was expressed in mammalian cells in which all 8 residues (Tyr-Pro-Pro-Trp-Asp-Lys-Asn-Phe) of the 60-loop were deleted (des-60-loop). Tyrosine 125-128 coagulation factor II, thrombin Homo sapiens 58-66 15548519-8 2005 Thrombin activated STAT-3 as measured by its tyrosine phosphorylation and translocation from the cytoplasm to the nucleus. Tyrosine 45-53 coagulation factor II, thrombin Homo sapiens 0-8 15548519-9 2005 Forced expression of a dominant negative mutant of STAT-3 reduced thrombin-induced STAT-3 tyrosine phosphorylation and its translocation from the cytoplasm to the nucleus. Tyrosine 90-98 coagulation factor II, thrombin Homo sapiens 66-74 15313016-0 2004 Sustained stimulation of platelet thrombin receptor is associated with tyrosine dephosphorylation of a novel p67 peptide in a manner regulated by extracellular calcium. Tyrosine 71-79 coagulation factor II, thrombin Homo sapiens 34-42 15313016-7 2004 Two proteins of relative mobilities 67 and 75 kD were found to be significantly dephosphorylated on tyrosine in calcium-pretreated platelets as compared to the EGTA-treated platelets following continued stimulation with either TRAP or thrombin for 15 min. Tyrosine 100-108 coagulation factor II, thrombin Homo sapiens 235-243 14978285-6 2004 Among residues constituting the interface, Phe-34, Ser-36A, Leu-65, Tyr-76, Arg-77A, Ile-82, and Lys-110 of thrombin and the A alpha chain Trp-33, Phe-35, Asp-38, Glu-39, the B beta chain Ala-68 and Asp-69, and the gamma chain Asp-27 and Ser-30 of E(ht) form a net of polar contacts surrounding a well defined hydrophobic interior. Tyrosine 68-71 coagulation factor II, thrombin Homo sapiens 108-116 14531686-2 2003 We followed the dissociation kinetics of the transient and specific complex of an antithrombotic peptide N-acetyl-Asp(55)-Phe-Glu-Glu-Ile-Pro(60)-Glu-Glu-Tyr-Leu-Gln(65) with human prothrombin by use of (15)N NMR relaxation dispersion spectroscopy of the peptide. Tyrosine 154-157 coagulation factor II, thrombin Homo sapiens 181-192 14598382-12 2003 Thrombin elicited tyrosine phosphorylation of lens epithelium membrane proteins, including a 100 kDa protein band thought to be the Na,K-ATPase alpha 1 subunit. Tyrosine 18-26 coagulation factor II, thrombin Homo sapiens 0-8 12794066-6 2003 Surface residues of thrombin further involved in high specificity fibrin-enhanced factor XIII activation were identified as His-66, Tyr-71, and Asn-74. Tyrosine 132-135 coagulation factor II, thrombin Homo sapiens 20-28 12847109-6 2003 We also demonstrated that platelet activation with thrombin induces tyrosine phosphorylation of CD226 as well as CD226-mediated platelet adhesion. Tyrosine 68-76 coagulation factor II, thrombin Homo sapiens 51-59 12846829-12 2003 Inhibition of platelet-platelet interactions and protein tyrosine phosphorylations by inhibitors of phosphatidylinositol 3-kinases and protein kinase C implies that activation of the latter are important steps of the GPIb-coupled signalling pathway triggered by thrombin. Tyrosine 57-65 coagulation factor II, thrombin Homo sapiens 262-270 12031920-9 2002 Tyrosine phosphorylation in platelets from patients with partial forms of SPD was equivalent to that observed in control platelets, absent in response to collagen and thrombin activation in delta-SPD, and deficient only to thrombin activation in alpha-SPD. Tyrosine 0-8 coagulation factor II, thrombin Homo sapiens 167-175 12603843-2 2003 Moreover, selective damage to dopaminergic neurones was produced after thrombin injection, evidenced by loss of tyrosine hydroxylase immunostaining and tyrosine hydroxylase mRNA-expressing cell bodies, and the unaltered transcription of glutamic acid decarboxylase mRNA in the SN and striatum. Tyrosine 112-120 coagulation factor II, thrombin Homo sapiens 71-79 12427016-8 2002 Strikingly, shaving at position 3, by Tyr --> Ala exchange, abolishes the differences in the affinity for thrombin allosteric forms, whereas a bulkier side chain (e.g., beta-naphthylalanine) improves binding preferentially to the fast form. Tyrosine 38-41 coagulation factor II, thrombin Homo sapiens 109-117 12411320-1 2002 The glycoprotein VI (GPVI)-specific agonist collagen-related peptide (CRP) and thrombin stimulated tyrosine phosphorylation of Vav1 but not Vav2 in human platelets. Tyrosine 99-107 coagulation factor II, thrombin Homo sapiens 79-87 12057926-1 2002 We have analyzed modifications on platelet ultrastructural morphology, cytoskeletal assembly, and tyrosine phosphorylation developing in platelets activated by both thrombin and the thrombin receptor-activating peptide (TRAP). Tyrosine 98-106 coagulation factor II, thrombin Homo sapiens 165-173 12031920-9 2002 Tyrosine phosphorylation in platelets from patients with partial forms of SPD was equivalent to that observed in control platelets, absent in response to collagen and thrombin activation in delta-SPD, and deficient only to thrombin activation in alpha-SPD. Tyrosine 0-8 coagulation factor II, thrombin Homo sapiens 223-231 11360180-8 2001 Besides its effect on tyrosine phosphorylation events, thrombin also triggered a rapid and robust redistribution of PKCepsilon and delta from the cytosol to the membrane. Tyrosine 22-30 coagulation factor II, thrombin Homo sapiens 55-63 11901150-3 2002 Thrombin recognizes fibrinogen with an extended binding surface, key elements of which are Tyr(76) in exosite I, located about 20 A away from the active site, and the aryl binding site located in close proximity to the catalytic triad. Tyrosine 91-94 coagulation factor II, thrombin Homo sapiens 0-8 11799111-8 2002 We obtained evidence that FAK can be activated by thrombin, Galpha(12), Galpha(13), and Galpha(q) through both Rho-dependent and Rho-independent mechanisms and that PDZ-RhoGEF and LARG can in turn be tyrosine-phosphorylated through FAK in response to thrombin, thereby enhancing the activation of Rho in vivo. Tyrosine 200-208 coagulation factor II, thrombin Homo sapiens 50-58 11594781-1 2001 We previously demonstrated that exposure of CCL39 lung fibroblasts to alpha-thrombin inhibits interleukin-6 (IL-6)-induced tyrosine phosphorylation of Stat3 (signal transducers and activators of transcription 3) via activation of mitogen-activated protein (MAP) kinase kinase 1 [Bhat et al. Tyrosine 123-131 coagulation factor II, thrombin Homo sapiens 76-84 11594781-8 2001 We demonstrate that, although both IL-6 and OSM belong to the same cytokine family, alpha-thrombin inhibited only the IL-6-induced Stat3 tyrosine phosphorylation. Tyrosine 137-145 coagulation factor II, thrombin Homo sapiens 90-98 11389697-2 2001 During aggregation by thrombin, cortactin associates with Src, is tyrosine phosphorylated, and then translocates to the cytoskeleton. Tyrosine 66-74 coagulation factor II, thrombin Homo sapiens 22-30 11278899-4 2001 In accordance with these data, pretreatment of human platelets with the JAK3 inhibitor WHI-P131 markedly decreased the base-line enzymatic activity of constitutively active JAK3 and abolished the thrombin-induced tyrosine phosphorylation of STAT1 and STAT3. Tyrosine 213-221 coagulation factor II, thrombin Homo sapiens 196-204 12112843-4 2002 In thrombin activated human platelets, however, there is a large increase in the number of tyrosine phosphorylated signalling proteins detected. Tyrosine 91-99 coagulation factor II, thrombin Homo sapiens 3-11 11756163-9 2002 PECAM-1 activation also inhibited platelet protein tyrosine phosphorylation stimulated by convulxin and thrombin; this was accompanied by inhibition of the mobilization of calcium from intracellular stores. Tyrosine 51-59 coagulation factor II, thrombin Homo sapiens 104-112 11311131-3 2001 When platelets were stimulated with thrombin, collagen or the stable thromboxane A(2) analogue U46619, Hic-5 was markedly tyrosine-phosphorylated, in a manner dependent on integrin alphaIIbbeta3-mediated aggregation. Tyrosine 122-130 coagulation factor II, thrombin Homo sapiens 36-44 11307817-3 2001 Tyrosine sulfation in these sequences enhances thrombin exosite binding, but this has not been uniformly investigated. Tyrosine 0-8 coagulation factor II, thrombin Homo sapiens 47-55 11042121-4 2000 During thrombin-induced platelet activation, Cbl was tyrosine phosphorylated, and phosphoinositide 3-kinase (PI 3-kinase) activity measured in Cbl immunoprecipitates was increased. Tyrosine 53-61 coagulation factor II, thrombin Homo sapiens 7-15 11110790-4 2001 Tyrosine phosphorylation of FAK caused by CRP-XL or thrombin, but not that caused by collagen fibers, was partially inhibited by GR144053F, an antagonist of integrin alpha(IIb)beta(3). Tyrosine 0-8 coagulation factor II, thrombin Homo sapiens 52-60 11020444-5 2000 SCH 79797 and its N-methyl analog (SCH 203099) inhibited binding of a high-affinity thrombin receptor-activating peptide ([(3)H]haTRAP, Ala-Phe(p-F)-Arg-ChA-HArg-[(3)H]Tyr-NH(2)) to PAR-1 with IC(50) values of 70 and 45 nM, respectively. Tyrosine 168-171 coagulation factor II, thrombin Homo sapiens 84-92 11092463-8 2000 Similar intracellular proteins were tyrosine phosphorylated after stimulation with thrombin, PAF and PMA. Tyrosine 36-44 coagulation factor II, thrombin Homo sapiens 83-91 10748008-1 2000 Regulatory exosite I of thrombin is present on prothrombin in a precursor state (proexosite I) that specifically binds the Tyr(63)-sulfated peptide, hirudin(54-65) (Hir(54-65)(SO(3)(-))) and the nonsulfated analog. Tyrosine 123-126 coagulation factor II, thrombin Homo sapiens 24-32 11034940-9 2000 CONCLUSIONS: Tyrosine protein phosphorylation is a regulatory control system of the inside-out mechanism of alpha(IIb)beta(3) activation and cytoskeletal assembly in thrombin-stimulated human platelets. Tyrosine 13-21 coagulation factor II, thrombin Homo sapiens 166-174 11034940-11 2000 Therefore, tyrosine phosphorylation is a major component of early signaling events and of COX-1-independent mechanisms of thrombin-induced platelet reactivity. Tyrosine 11-19 coagulation factor II, thrombin Homo sapiens 122-130 10748008-1 2000 Regulatory exosite I of thrombin is present on prothrombin in a precursor state (proexosite I) that specifically binds the Tyr(63)-sulfated peptide, hirudin(54-65) (Hir(54-65)(SO(3)(-))) and the nonsulfated analog. Tyrosine 123-126 coagulation factor II, thrombin Homo sapiens 47-58 10820165-10 2000 An increased level of tyrosine-phosphorylated c-src suggested the activation of this cytoplasmic tyrosine kinase in response to thrombin and its potential role in thrombin-induced protein-tyrosine phosphorylation. Tyrosine 22-30 coagulation factor II, thrombin Homo sapiens 128-136 10820165-10 2000 An increased level of tyrosine-phosphorylated c-src suggested the activation of this cytoplasmic tyrosine kinase in response to thrombin and its potential role in thrombin-induced protein-tyrosine phosphorylation. Tyrosine 22-30 coagulation factor II, thrombin Homo sapiens 163-171 10820165-10 2000 An increased level of tyrosine-phosphorylated c-src suggested the activation of this cytoplasmic tyrosine kinase in response to thrombin and its potential role in thrombin-induced protein-tyrosine phosphorylation. Tyrosine 97-105 coagulation factor II, thrombin Homo sapiens 128-136 10820165-10 2000 An increased level of tyrosine-phosphorylated c-src suggested the activation of this cytoplasmic tyrosine kinase in response to thrombin and its potential role in thrombin-induced protein-tyrosine phosphorylation. Tyrosine 97-105 coagulation factor II, thrombin Homo sapiens 163-171 10749687-3 2000 When platelets were stimulated with thrombin, Pyk2, as well as FAK, was markedly tyrosine-phosphorylated, in a manner mostly dependent on alphaIIbbeta3 integrin-mediated aggregation. Tyrosine 81-89 coagulation factor II, thrombin Homo sapiens 36-44 10794720-4 2000 Here we report biochemical and pharmacological evidence that the tyrosine kinase pp60(c-src) is constitutively associated with SHIP1 and is involved in its tyrosine phosphorylation downstream of integrin engagement in thrombin-activated human platelets. Tyrosine 65-73 coagulation factor II, thrombin Homo sapiens 218-226 10677232-3 2000 Factor Xa (FXa), the serine protease of the prothrombinase complex, contains a Tyr at this position. Tyrosine 79-82 coagulation factor II, thrombin Homo sapiens 21-36 10704823-3 2000 Here, we show that p72(Syk) and FcgammaRII tyrosine phosphorylation and association occured following platelet stimulation by: (1) two monoclonal antibodies, which form a bridge between a target antigen and FcgammaRII, and (2) the G-protein-coupled receptor agonist thrombin. Tyrosine 43-51 coagulation factor II, thrombin Homo sapiens 266-274 10681592-4 2000 Thrombin treatment of human umbilical vein endothelial cells promotes SHP2 tyrosine phosphorylation and dissociation from VE-cadherin complexes. Tyrosine 75-83 coagulation factor II, thrombin Homo sapiens 0-8 10681592-6 2000 We propose that thrombin regulates the tyrosine phosphorylation of VE-cadherin-associated beta-catenin, gamma-catenin, and p120-catenin by modulating the quantity of SHP2 associated with VE-cadherin complexes. Tyrosine 39-47 coagulation factor II, thrombin Homo sapiens 16-24 10739383-3 2000 This system, in the presence of 100 microM L-tyrosine, caused in the thrombin molecule loss of tryptophan and lysine residues and formation of dityrosine, chloramine and carbonyl groups. Tyrosine 43-53 coagulation factor II, thrombin Homo sapiens 69-77 10508911-0 1999 Thrombin-induced association of SHP-2 with multiple tyrosine-phosphorylated proteins in human platelets. Tyrosine 52-60 coagulation factor II, thrombin Homo sapiens 0-8 10642282-3 2000 More recently, we discovered that PMCA is phosphorylated on tyrosine residues during thrombin-stimulated platelet aggregation and that this phosphorylation causes inhibition of PMCA activity. Tyrosine 60-68 coagulation factor II, thrombin Homo sapiens 85-93 10739244-7 2000 Together, the aromatic rings of the C-2 and C-3 side chains sandwich the indole ring of Trp60D contained in the thrombin S2 insertion loop defined by the sequence "Tyr-Pro-Pro-Trp." Tyrosine 164-167 coagulation factor II, thrombin Homo sapiens 112-120 10598576-4 1999 By mutating the tyrosines in Shc, we show that the three sites of tyrosine phosphorylation, Y239, Y240, and Y317, are necessary for thrombin signaling in both systems. Tyrosine 16-25 coagulation factor II, thrombin Homo sapiens 132-140 10598576-4 1999 By mutating the tyrosines in Shc, we show that the three sites of tyrosine phosphorylation, Y239, Y240, and Y317, are necessary for thrombin signaling in both systems. Tyrosine 16-24 coagulation factor II, thrombin Homo sapiens 132-140 10080949-3 1999 Interestingly, a short pretreatment of cells with alpha-thrombin significantly inhibited IL-6-induced tyrosine phosphorylation of Stat3. Tyrosine 102-110 coagulation factor II, thrombin Homo sapiens 56-64 10464282-2 1999 Comparison of protein C activation in the presence and absence of TM identified 11 residues mediating the thrombin-TM interaction (Lys(21), Gln(24), Arg(62), Lys(65), His(66), Arg(68), Thr(69), Tyr(71), Arg(73), Lys(77), Lys(106)). Tyrosine 194-197 coagulation factor II, thrombin Homo sapiens 106-114 10093970-3 1999 While PYK2 tyrosine phosphorylation induced by thrombin was inhibited by preincubation of platelets with PKC inhibitors, staurosporine and Ro31-8220, PYK2 association with Src was markedly enhanced under the same conditions. Tyrosine 11-19 coagulation factor II, thrombin Homo sapiens 47-55 10051558-6 1999 The activity profiles obtained for thrombin also suggest that the conversion of Pro to Tyr or Phe documented in the vertebrates occurred through Ser and was driven by a significant gain (up to 50-fold) in catalytic activity. Tyrosine 87-90 coagulation factor II, thrombin Homo sapiens 35-43 12774450-7 1999 Using two-dimensional immunoblotting we demonstrate that thrombin stimulation leads to increases in the tyrosine phosphorylation of 4 proteins, three different isoforms of P44 mitogen activated protein kinase (MAPK) and one isoform of P38 stress activated protein kinase (SAPK). Tyrosine 104-112 coagulation factor II, thrombin Homo sapiens 57-65 10026222-2 1999 Here we report that CRP and the platelet low affinity immune receptor FcgammaRIIA stimulate tyrosine phosphorylation of the T cell adapter SLP-76, whereas the G protein-coupled receptor agonist thrombin induces only minor tyrosine phosphorylation. Tyrosine 222-230 coagulation factor II, thrombin Homo sapiens 194-202 9928954-2 1999 Here we show that Btk is activated and undergoes tyrosine phosphorylation upon challenge of platelet thrombin receptor, these responses requiring engagement of alphaIIb/beta3 integrin and phosphoinositide 3-kinase activity. Tyrosine 49-57 coagulation factor II, thrombin Homo sapiens 101-109 10091587-4 1999 First, practically all protein-tyrosine phosphorylation induced by thrombin was inhibited by IAS, while a partial inhibition was observed for collagen; the phosphorylation due to collagen alone was apparently stimulated by elevation of cAMP. Tyrosine 31-39 coagulation factor II, thrombin Homo sapiens 67-75 16801121-1 1999 Thrombin activation of human platelets is accompanied by tyrosine phosphorylation of many cellular proteins, as well as changes in the cytosolic Ca2+ concentration. Tyrosine 57-65 coagulation factor II, thrombin Homo sapiens 0-8 16801121-3 1999 Stimulation of platelets with thrombin resulted in tyrosine phosphorylation of the type 1 IP(3)R, which peaked at 3 min followed by dephosphorylation to the basal level by 10 min. Tyrosine 51-59 coagulation factor II, thrombin Homo sapiens 30-38 9731041-9 1998 Although thrombin- and thrombopoietin-induced increase in tyrosine phosphorylation of WASP is negligible or marginal, WASP from thrombin-activated platelets became incorporated into the Triton X-100-insoluble 10, 000g sedimentable residue in an aggregation-dependent manner, suggesting that it may have a regulatory role in platelet cytoskeletal processes during aggregation. Tyrosine 58-66 coagulation factor II, thrombin Homo sapiens 9-17 9891970-4 1998 In this study, we demonstrated that LAT is present in platelets and is tyrosine phosphorylated in response to ADP- and thrombin-stimulated aggregation. Tyrosine 71-79 coagulation factor II, thrombin Homo sapiens 119-127 9831706-13 1998 These data indicate that in endothelial cell monolayers that respond to histamine in a physiological way, thrombin induces a prolonged increase in permeability by "calcium sensitization," which involves protein tyrosine phosphorylation and RhoA activation. Tyrosine 211-219 coagulation factor II, thrombin Homo sapiens 106-114 9792114-2 1998 In preliminary studies we observed that high concentrations (30 microM) of a protein kinase C inhibitor, Ro 31-8220, selectively suppressed p130 tyrosine phosphorylation induced by thrombin, the thromboxane synthetic analogue (U46619) and ADP. Tyrosine 145-153 coagulation factor II, thrombin Homo sapiens 181-189 9813145-1 1998 The platelet phosphotyrosine phosphatase (PTP) SHP-1 is tyrosine phosphorylated during thrombin-induced activation. Tyrosine 20-28 coagulation factor II, thrombin Homo sapiens 87-95 9813145-4 1998 When SHP-1 was tyrosine phosphorylated by thrombin, further addition of A23187 failed to induce its cleavage. Tyrosine 15-23 coagulation factor II, thrombin Homo sapiens 42-50 9813145-5 1998 In the presence of tyrphostin to inhibit thrombin-induced SHP-1 tyrosine phosphorylation, SHP-1 was cleaved. Tyrosine 64-72 coagulation factor II, thrombin Homo sapiens 41-49 9753458-0 1998 Substitution of tyrosine for phenylalanine in fibrinopeptide A results in preferential thrombin cleavage of fibrinopeptide B from fibrinogen. Tyrosine 16-24 coagulation factor II, thrombin Homo sapiens 87-95 9582299-2 1998 In thrombin-induced protection of astrocytes from hypoglycemia, pretreatment of astrocytes with tyrosine or serine/threonine kinase inhibitors, cytochalasin D, or exoenzyme C3, a potent inhibitor of the small GTPase RhoA, attenuated thrombin-induced protection. Tyrosine 96-104 coagulation factor II, thrombin Homo sapiens 3-11 9652744-4 1998 Tec was rapidly tyrosine phosphorylated in response to platelet agonists which activate G protein-coupled receptors such as thromboxane A2 analog (U46619), thrombin, and thrombin receptor activating peptide (TRAP). Tyrosine 16-24 coagulation factor II, thrombin Homo sapiens 156-164 9677317-0 1998 Microvesicle release is associated with extensive protein tyrosine dephosphorylation in platelets stimulated by A23187 or a mixture of thrombin and collagen. Tyrosine 58-66 coagulation factor II, thrombin Homo sapiens 135-143 9596671-2 1998 We have previously shown that thrombin stimulation of the T-leukemic cell line Jurkat induced a transient increase in [Ca2+]i and tyrosine phosphorylation of several cellular proteins. Tyrosine 130-138 coagulation factor II, thrombin Homo sapiens 30-38 9596671-6 1998 Tyrosine phosphorylation of p38 MAPK was drastically increased after thrombin stimulation of JCaM1 or J45.01 cells, as compared with parental cells (JE6.1). Tyrosine 0-8 coagulation factor II, thrombin Homo sapiens 69-77 9596671-11 1998 Accordingly, we found that thrombin enhanced tyrosine phosphorylation of p56Lck and decreased p56Lck kinase activity in J45.01 cells. Tyrosine 45-53 coagulation factor II, thrombin Homo sapiens 27-35 9593734-1 1998 Tyrosine phosphorylation of the beta3 subunit of the major platelet integrin alphaIIb beta3 has been shown to occur during thrombin-induced platelet aggregation (1). Tyrosine 0-8 coagulation factor II, thrombin Homo sapiens 123-131 9475764-8 1998 Thrombin-mediated tyrosine phosphorylation of proteins was intact in platelets from patients with polycythemia vera, and the tyrosine kinases and substrates involved in the process were present in normal amounts. Tyrosine 18-26 coagulation factor II, thrombin Homo sapiens 0-8 9588188-1 1998 Thrombin elicits responses in platelets such as shape change, aggregation, arachidonate liberation and secretion of the contents of three storage granules, processes that coincide with serine/threonine and tyrosine phosphorylation of numerous proteins, hydrolysis of polyphosphoinositides and mobilisation of Ca2+ within the cell. Tyrosine 206-214 coagulation factor II, thrombin Homo sapiens 0-8 9588188-6 1998 When the thrombin-induced tyrosine phosphorylations was corrected for RGDS-induced phosphorylation, the presence of inhibitors of autocrine stimulation reduced the thrombin-induced phosphorylation by 97%. Tyrosine 26-34 coagulation factor II, thrombin Homo sapiens 9-17 9588188-6 1998 When the thrombin-induced tyrosine phosphorylations was corrected for RGDS-induced phosphorylation, the presence of inhibitors of autocrine stimulation reduced the thrombin-induced phosphorylation by 97%. Tyrosine 26-34 coagulation factor II, thrombin Homo sapiens 164-172 9341117-0 1997 Tyrosine phosphorylation and relocation of SHIP are integrin-mediated in thrombin-stimulated human blood platelets. Tyrosine 0-8 coagulation factor II, thrombin Homo sapiens 73-81 9459347-5 1998 rHuTPO stimulated and potentiated the thrombin-induced tyrosine phosphorylation of a 80 kDa protein identified as the cortical actin-associated protein, p80/85 cortactin. Tyrosine 55-63 coagulation factor II, thrombin Homo sapiens 38-46 11012190-6 1998 Thrombin-mediated platelet protein tyrosine phosphorylation was intact in PV platelets as was expression of the appropriate tyrosine kinases and their cognate substrates. Tyrosine 35-43 coagulation factor II, thrombin Homo sapiens 0-8 9341117-6 1997 Thrombin stimulation induced a tyrosine phosphorylation of SHIP, this effect being prevented if platelets were not shaken or if RGD-containing peptides were present, indicating an aggregation-dependent, integrin-mediated event. Tyrosine 31-39 coagulation factor II, thrombin Homo sapiens 0-8 9342235-3 1997 In human platelets, thrombin induces the relocation of Syk to the cytoskeletal fraction presumably via Syk tyrosine phosphorylation. Tyrosine 107-115 coagulation factor II, thrombin Homo sapiens 20-28 9271811-5 1997 On the contrary, iodination at Tyr-3 caused dual effects on the interaction between CX-397 and alpha-thrombin; namely, mono-iodination at Tyr-3 caused a small decrease in Koff (1.29- to 1.63-fold) than non-iodinated ones, whilst di-iodination at Tyr-3 brought about 3.04- to 3.88-fold increase in Koff. Tyrosine 31-34 coagulation factor II, thrombin Homo sapiens 101-109 9299487-0 1997 Integrin-dependent tyrosine phoshorylation and cytoskeletal translocation of Tec in thrombin-activated platelets. Tyrosine 19-27 coagulation factor II, thrombin Homo sapiens 84-92 9299487-4 1997 The tetrapeptide RGDS inhibited more than 90% of thrombin-induced tyrosine phosphorylation of Tec and blocked its translocation to the cytoskeleton. Tyrosine 66-74 coagulation factor II, thrombin Homo sapiens 49-57 9271811-5 1997 On the contrary, iodination at Tyr-3 caused dual effects on the interaction between CX-397 and alpha-thrombin; namely, mono-iodination at Tyr-3 caused a small decrease in Koff (1.29- to 1.63-fold) than non-iodinated ones, whilst di-iodination at Tyr-3 brought about 3.04- to 3.88-fold increase in Koff. Tyrosine 138-141 coagulation factor II, thrombin Homo sapiens 101-109 9271811-5 1997 On the contrary, iodination at Tyr-3 caused dual effects on the interaction between CX-397 and alpha-thrombin; namely, mono-iodination at Tyr-3 caused a small decrease in Koff (1.29- to 1.63-fold) than non-iodinated ones, whilst di-iodination at Tyr-3 brought about 3.04- to 3.88-fold increase in Koff. Tyrosine 138-141 coagulation factor II, thrombin Homo sapiens 101-109 9192762-7 1997 These results suggest that tyrosine phosphorylation of the 77 to 80 kD doublet, identified by immunoprecipitation as the cytoskeletal protein cortactin, and the 64 kD band are dependent both on thrombin activation during early steps and on the late steps of alphaIIb beta3 engagement in aggregation. Tyrosine 27-35 coagulation factor II, thrombin Homo sapiens 194-202 9247313-4 1997 As has been reported in other systems, thrombin stimulation results in tyrosine phosphorylation of Shc in 1321N1 cells. Tyrosine 71-79 coagulation factor II, thrombin Homo sapiens 39-47 9099753-5 1997 Treatment of platelets with thrombin induced, in as early as 10 s, a rapid tyrosine phosphorylation of RAFTK in a time- and concentration-dependent manner. Tyrosine 75-83 coagulation factor II, thrombin Homo sapiens 28-36 9182531-5 1997 Treatment with thrombin evoked tyrosine phosphorylation of platelet PMCA, while PMCA from resting platelets exhibited little tyrosine phosphorylation. Tyrosine 31-39 coagulation factor II, thrombin Homo sapiens 15-23 9182576-6 1997 Thrombin and the phorbol ester, phorbol 12-myristate 13-acetate, also increased p125(FAK) tyrosine phosphorylation in HUVECs. Tyrosine 90-98 coagulation factor II, thrombin Homo sapiens 0-8 9108397-0 1997 Thrombopoietin and thrombin induce tyrosine phosphorylation of Vav in human blood platelets. Tyrosine 35-43 coagulation factor II, thrombin Homo sapiens 19-27 9081683-11 1997 Furthermore, stimulation with histamine, thrombin, or pervanadate all caused tyrosine phosphorylation of a mitogen-activated protein kinase (ERK1/p44). Tyrosine 77-85 coagulation factor II, thrombin Homo sapiens 41-49 9081683-8 1997 While the tyrosine phosphorylation of PLC gamma 1 was enhanced after cotreatment with thrombin and pervanadate compared with pervanadate alone, costimulation with pervanadate and histamine resulted in no more tyrosine phosphorylation of PLC gamma 1 than after pervanadate alone. Tyrosine 10-18 coagulation factor II, thrombin Homo sapiens 86-94 9081683-13 1997 Moreover, thrombin- but not histamine-induced generation of IPs appears to be partly caused by tyrosine phosphorylation of PLC gamma 1. Tyrosine 95-103 coagulation factor II, thrombin Homo sapiens 10-18 9089407-0 1997 Tyrosine phosphorylation and Syk activation are involved in thrombin-induced aggregation of epinephrine-potentiated platelets. Tyrosine 0-8 coagulation factor II, thrombin Homo sapiens 60-68 9089407-4 1997 Although epinephrine alone (4 microM) slightly induced protein-tyrosine phosphorylation and Syk activation, the presence of epinephrine caused a shift to the left in the dose-dependence of thrombin (0.01-0.5 U/ml)-induced tyrosine phosphorylation and Syk activation, as well as platelet aggregation. Tyrosine 222-230 coagulation factor II, thrombin Homo sapiens 189-197 9089407-2 1997 On the other hand, tyrosine phosphorylation and activation of tyrosine kinases including Syk have been shown to play a critical role in the induction of platelet responses to thrombin stimulation. Tyrosine 19-27 coagulation factor II, thrombin Homo sapiens 175-183 9089407-3 1997 This study investigated the role of tyrosine phosphorylation and Syk activation in the synergistic mechanisms between thrombin and epinephrine. Tyrosine 36-44 coagulation factor II, thrombin Homo sapiens 118-126 9034245-5 1997 The same pattern of tyrosine phosphorylation, but with higher intensity, was induced by thrombin in control platelets. Tyrosine 20-28 coagulation factor II, thrombin Homo sapiens 88-96 9067640-4 1997 Since thrombin-induced tyrosine phosphorylations were completely inhibited in the presence of okadaic acid, as a consequence, phosphatidylinositol 3-kinase was no longer detected in antiphosphotyrosine immunoprecipitates, thus explaining the absence of phosphatidylinositol, 3,4-bisphosphate synthesis. Tyrosine 23-31 coagulation factor II, thrombin Homo sapiens 6-14 9034245-6 1997 Pretreatment of platelets with peroxynitrite decreased thrombin-induced tyrosine phosphorylation at 0.05 and 1 U/ml thrombin but not at 2 U/ml thrombin. Tyrosine 72-80 coagulation factor II, thrombin Homo sapiens 116-124 9034245-6 1997 Pretreatment of platelets with peroxynitrite decreased thrombin-induced tyrosine phosphorylation at 0.05 and 1 U/ml thrombin but not at 2 U/ml thrombin. Tyrosine 72-80 coagulation factor II, thrombin Homo sapiens 55-63 9034245-8 1997 Peroxynitrite exposure and tyrosine nitration decreased platelet sensitivity to thrombin but did not absolutely prevent tyrosine phosphorylation and other platelet responses. Tyrosine 27-35 coagulation factor II, thrombin Homo sapiens 80-88 9034245-6 1997 Pretreatment of platelets with peroxynitrite decreased thrombin-induced tyrosine phosphorylation at 0.05 and 1 U/ml thrombin but not at 2 U/ml thrombin. Tyrosine 72-80 coagulation factor II, thrombin Homo sapiens 116-124 8798504-5 1996 The tyrosine-sulfated, thrombin exosite-binding hirudin peptide, S-Hir53-64 (hirugen), inhibited both low and high affinity thrombin binding to fibrin (IC50 1.4 and 3.0 microM respectively). Tyrosine 4-12 coagulation factor II, thrombin Homo sapiens 23-31 8943867-4 1996 Before and after stimulation with thrombin, the only consistent difference between normal and CML platelets was the presence of a tyrosine phosphorylated protein with a relative molecular weight of 39 kD. Tyrosine 130-138 coagulation factor II, thrombin Homo sapiens 34-42 8972712-0 1996 Endogenous ADP prevents PGE1-induced tyrosine dephosphorylation of focal adhesion kinase in thrombin-activated platelets. Tyrosine 37-45 coagulation factor II, thrombin Homo sapiens 92-100 8972712-1 1996 Prostaglandin E1(PGE1) inhibits tyrosine phosphorylation induced by low thrombin concentration (0.05 U/ml), but this is overcome by a high thrombin (2.0 U/ml) concentration. Tyrosine 32-40 coagulation factor II, thrombin Homo sapiens 72-80 8972712-3 1996 We investigated how these endogenous agonists influenced the effects of PGE1 on thrombin (2.0 U/ml)-induced tyrosine phosphorylation by removing released ADP with apyrase (2.0 U/ml) and by inhibiting thromboxane A2 synthesis with indomethacin (1 microM). Tyrosine 108-116 coagulation factor II, thrombin Homo sapiens 80-88 8972712-4 1996 Adding PGE1 (1 microM) before thrombin in apyrase/indomethacin(A/I)-treated platelets selectively prevented thrombin-induced tyrosine phosphorylation of a 117 kDa protein while other substrates were not affected. Tyrosine 125-133 coagulation factor II, thrombin Homo sapiens 108-116 8972712-7 1996 Conditions which prevented thrombin-induced 117 kDa protein tyrosine phosphorylation also decreased fibrinogen binding to platelets. Tyrosine 60-68 coagulation factor II, thrombin Homo sapiens 27-35 9076944-9 1997 An anionic/sulfated tyrosine sequence of the GP Ib alpha-chain that is critical for binding of the GP Ib-IX-V complex to both vWF and alpha-thrombin is analogous to sulfated anionic amino acid sequences mediating interactions of other adhesive proteins, including P-selectin binding to PSGL-1 and Factor VIII binding to vWF. Tyrosine 20-28 coagulation factor II, thrombin Homo sapiens 140-148 9031466-2 1997 Upon thrombin stimulation of human platelets, SHP-1 is rapidly phosphorylated on both serine and tyrosine residues, and becomes associated with the cytoskeleton, where it could participate in the formation of multiprotein signalling complexes. Tyrosine 97-105 coagulation factor II, thrombin Homo sapiens 5-13 8798504-5 1996 The tyrosine-sulfated, thrombin exosite-binding hirudin peptide, S-Hir53-64 (hirugen), inhibited both low and high affinity thrombin binding to fibrin (IC50 1.4 and 3.0 microM respectively). Tyrosine 4-12 coagulation factor II, thrombin Homo sapiens 124-132 8732754-15 1996 The higher degree of specificity for trypsin is most likely the result of these substitutions and the absence of the fairly rigid Tyr 60A-Thr 60I insertion loop of thrombin, which narrows access to the active site and forces less favorable orientations for the D-Phe and v-Tyr residues. Tyrosine 130-133 coagulation factor II, thrombin Homo sapiens 164-172 8695800-7 1996 Tyrosine phosphorylation of p38 and p63 is also observed in platelets stimulated by the tyrosine kinase-linked receptor agonist collagen and by the G protein-coupled receptor agonist thrombin, although phosphorylation of SLP-76 is only observed in collagen-stimulated platelets. Tyrosine 0-8 coagulation factor II, thrombin Homo sapiens 183-191 8662746-4 1996 Tyrosine phosphorylation of Raf-1 is induced by FcgammaRI activation and not by PMA (1 microg/ml), N-formyl-Met-Leu-Phe (1 microM), calcium ionophore (1 microM), thrombin (0.05 unit/ml), FcgammaRII, or FcgammaRIII stimulation. Tyrosine 0-8 coagulation factor II, thrombin Homo sapiens 162-170 8690082-2 1996 PD98059 dose-dependently attenuated the tyrosine phosphorylation and activation of p42 mapk in response to thrombin or inflammatory cytokines. Tyrosine 40-48 coagulation factor II, thrombin Homo sapiens 107-115 8631894-5 1996 In this paper we demonstrate that the beta 3 subunit of alpha IIb beta 3 was phosphorylated on tyrosine residues in response to thrombin-induced platelet aggregation. Tyrosine 95-103 coagulation factor II, thrombin Homo sapiens 128-136 8732754-15 1996 The higher degree of specificity for trypsin is most likely the result of these substitutions and the absence of the fairly rigid Tyr 60A-Thr 60I insertion loop of thrombin, which narrows access to the active site and forces less favorable orientations for the D-Phe and v-Tyr residues. Tyrosine 273-276 coagulation factor II, thrombin Homo sapiens 164-172 8615807-8 1996 At concentrations that abolished PGI2 release, genistein blocked thrombin- or histamine-evoked tyrosine phosphorylation of a 42 kDa protein. Tyrosine 95-103 coagulation factor II, thrombin Homo sapiens 65-73 8664285-1 1996 Identification of the sulfated tyrosine/anionic sequence Tyr-276-Glu-282 of glycoprotein Ibalpha as a binding site for von Willebrand factor and alpha-thrombin. Tyrosine 31-39 coagulation factor II, thrombin Homo sapiens 151-159 8664285-1 1996 Identification of the sulfated tyrosine/anionic sequence Tyr-276-Glu-282 of glycoprotein Ibalpha as a binding site for von Willebrand factor and alpha-thrombin. Tyrosine 57-60 coagulation factor II, thrombin Homo sapiens 151-159 8664285-16 1996 These findings indicate that the sulfated tyrosine/anionic GP Ibalpha residues Tyr-276-Glu-282 are important for the binding of thrombin and botrocetin-dependent binding of thrombin and the botrocetin-dependent binding of vWF, but that vWF also interacts with residues within His-1-Leu-275. Tyrosine 42-50 coagulation factor II, thrombin Homo sapiens 128-136 8664285-16 1996 These findings indicate that the sulfated tyrosine/anionic GP Ibalpha residues Tyr-276-Glu-282 are important for the binding of thrombin and botrocetin-dependent binding of thrombin and the botrocetin-dependent binding of vWF, but that vWF also interacts with residues within His-1-Leu-275. Tyrosine 42-50 coagulation factor II, thrombin Homo sapiens 173-181 8704107-4 1996 This study reports the effects of cAMP and thrombin on mesangial cell shape, distribution of actin, formation of stress fibers, and tyrosine phosphorylation of p125FAK. Tyrosine 132-140 coagulation factor II, thrombin Homo sapiens 43-51 8605873-4 1996 Here we report that the adaptor protein Shc, is phosphorylated on tyrosine residues following stimulation of the thrombin receptor in growth-responsive CCL39 fibroblasts. Tyrosine 66-74 coagulation factor II, thrombin Homo sapiens 113-121 8704107-0 1996 Tyrosine phosphorylation of focal adhesion kinase (p125FAK): regulation by cAMP and thrombin in mesangial cells. Tyrosine 0-8 coagulation factor II, thrombin Homo sapiens 84-92 8631786-0 1996 Thrombin receptor activation and integrin engagement stimulate tyrosine phosphorylation of the proto-oncogene product, p95vav, in platelets. Tyrosine 63-71 coagulation factor II, thrombin Homo sapiens 0-8 8631786-5 1996 Two potent platelet agonists, thrombin (via its G protein-coupled receptor) and collagen (via its interaction with the alpha2beta1 integrin), caused Vav to become phosphorylated on tyrosine. Tyrosine 181-189 coagulation factor II, thrombin Homo sapiens 30-38 8704107-6 1996 Addition of thrombin to these cells restores actin filaments (stress fibers) and increases tyrosine phosphorylation of p125FAK, and the cells resume a flattened morphology, even in the continued presence of cAMP-elevating agents. Tyrosine 91-99 coagulation factor II, thrombin Homo sapiens 12-20 8779890-1 1996 Thrombin stimulates mitogenesis and tyrosine phosphorylation of several proteins in glomerular mesangial cells [T. Force, J. M. Kyriakis, J. Avruch, and J. V. Bonventre, J. Biol. Tyrosine 36-44 coagulation factor II, thrombin Homo sapiens 0-8 8779890-9 1996 Immunoblot analysis of antiphosphotyrosine immunoprecipitates from lysates of thrombin-treated cells with anti-Nck antibody revealed the presence of this src homology domain-containing adaptor molecule in the tyrosine-phosphorylated protein fraction. Tyrosine 34-42 coagulation factor II, thrombin Homo sapiens 78-86 8603016-0 1996 Presence of the seven transmembrane thrombin receptor on human tumour cells: effect of activation on tumour adhesion to platelets and tumor tyrosine phosphorylation. Tyrosine 140-148 coagulation factor II, thrombin Homo sapiens 36-44 8603016-6 1996 Four of the six cell lines undergo tyrosine phosphorylation within 30 s to 1 min after exposure to 6-mer TRAP or thrombin. Tyrosine 35-43 coagulation factor II, thrombin Homo sapiens 113-121 7499191-6 1995 Further, we demonstrate that activation of this kinase by thrombin is transient (with maximal stimulation at 1 min), is accompanied by tyrosine phosphorylation, and precedes the activation of the ERK kinases. Tyrosine 135-143 coagulation factor II, thrombin Homo sapiens 58-66 8944416-7 1996 Okadaic acid inhibits thrombin-induced tyrosine phosphorylation in a concentration-dependent manner. Tyrosine 39-47 coagulation factor II, thrombin Homo sapiens 22-30 8944416-10 1996 Furthermore, we also found that thrombin-induced tyrosine phosphorylation was markedly inhibited in the presence of low concentrations of both okadaic acid and PGI2, therefore explaining the synergistic inhibition of platelet aggregation and secretion. Tyrosine 49-57 coagulation factor II, thrombin Homo sapiens 32-40 8547151-12 1995 No correlation was noted between inhibition of fibrinogen binding, location of MoAb binding on GPIIIa, reactivity of MoAb binding with GPIIIa, inhibition of thrombin-induced tyrosine phosphorylation or inhibition of platelet aggregation induced by ADP, collagen or thrombin. Tyrosine 174-182 coagulation factor II, thrombin Homo sapiens 157-165 7758170-8 1995 Thrombin stimulated a rapid increase in phosphotyrosine-containing proteins, suggesting a role for tyrosine phosphorylation in thrombin signaling. Tyrosine 47-55 coagulation factor II, thrombin Homo sapiens 0-8 7499242-13 1995 Treatment of the megakaryocytic CMK cells with thrombin caused a rapid induction of tyrosine phosphorylation of RAFTK protein. Tyrosine 84-92 coagulation factor II, thrombin Homo sapiens 47-55 7758170-8 1995 Thrombin stimulated a rapid increase in phosphotyrosine-containing proteins, suggesting a role for tyrosine phosphorylation in thrombin signaling. Tyrosine 47-55 coagulation factor II, thrombin Homo sapiens 127-135 7482416-7 1995 At least 7 tyrosine-phosphorylated proteins are detected upon stimulation of platelets by thrombin. Tyrosine 11-19 coagulation factor II, thrombin Homo sapiens 90-98 7781604-0 1995 Tyrosine phosphorylation of an SH2-containing protein tyrosine phosphatase is coupled to platelet thrombin receptor via a pertussis toxin-sensitive heterotrimeric G-protein. Tyrosine 0-8 coagulation factor II, thrombin Homo sapiens 98-106 7781604-2 1995 Here we report that SH-PTP1 is phosphorylated on both serine and tyrosine residues in response to thrombin or phorbol myristate acetate (PMA), which increased by 60 and 40%, respectively, SH-PTP1 activity. Tyrosine 65-73 coagulation factor II, thrombin Homo sapiens 98-106 7781604-4 1995 Moreover, in contrast with PMA, the effect of thrombin on the tyrosine phosphorylation of SH-PTP1 was hardly affected by GF109203X, a specific protein kinase C (PKC) inhibitor. Tyrosine 62-70 coagulation factor II, thrombin Homo sapiens 46-54 7781604-8 1995 This might offer some clue to unravel the mechanism of thrombin not only in platelets but also in nucleated cells, where its mitogenic effect is known to involve pertussis toxin-sensitive G-proteins, tyrosine phosphorylation and the ras pathway. Tyrosine 200-208 coagulation factor II, thrombin Homo sapiens 55-63 7537275-8 1995 This association with F-actin appeared to be at least partly indirect, since we demonstrated a thrombin-dependent interaction of p85 alpha with a proline-rich sequence of the tyrosine-phosphorylated cytoskeletal focal adhesion kinase, p125FAK. Tyrosine 175-183 coagulation factor II, thrombin Homo sapiens 95-103 8587330-6 1995 Phosphotyrosine immunoblot assays show that calcium/calmodulin-dependent signaling pathways precede thrombin-induced tyrosine phosphorylation, and that the calcium/calmodulin-dependent phosphatase calcineurin also exerts its effects via activation of protein tyrosine kinases. Tyrosine 7-15 coagulation factor II, thrombin Homo sapiens 100-108 7721887-6 1995 Fragments containing a single Tyr-->Phe substitution either at position 276 or 278 or 279 exhibited normal interaction with vWF but markedly reduced or absent binding of alpha-thrombin. Tyrosine 30-33 coagulation factor II, thrombin Homo sapiens 179-187 7532010-3 1995 Here we show that almost identical patterns of phosphotyrosine-containing proteins are detectable in the cytoskeleton after platelet stimulation with compounds that directly (phorbol 12-myristate, 13-acetate) or indirectly (thrombin, vasopressin, collagen, ADP) activate protein kinase C. The apparent molecular masses of the proteins phosphorylated at tyrosine residues are 145, 130, 100, 85, 80, 60, 56, 54 and 38 kDa. Tyrosine 54-62 coagulation factor II, thrombin Homo sapiens 224-232 7532010-5 1995 Concentrations of thrombin as low as 0.01 units per ml are able to cause tyrosine phosphorylation of multiple proteins. Tyrosine 73-81 coagulation factor II, thrombin Homo sapiens 18-26 7532010-6 1995 The time course of protein tyrosine phosphorylation for thrombin- and vasopressin-stimulated platelets revealed a rapid increase in the cytoskeleton within 5 to 20 s following activation consistent with a role in early events of platelet function. Tyrosine 27-35 coagulation factor II, thrombin Homo sapiens 56-64 7721887-0 1995 Identification of three tyrosine residues of glycoprotein Ib alpha with distinct roles in von Willebrand factor and alpha-thrombin binding. Tyrosine 24-32 coagulation factor II, thrombin Homo sapiens 122-130 7537105-2 1995 It is of interest that 75-80, 97 and 125 kDa proteins which are strongly tyrosine-phosphorylated in platelet activation induced by thrombin and other agonists were not detected. Tyrosine 73-81 coagulation factor II, thrombin Homo sapiens 131-139 7749863-2 1995 In platelets stimulated by 1 U/mL thrombin, protein-tyrosine phosphorylation was maximal after 2 minutes and was followed by protein-tyrosine dephosphorylation. Tyrosine 52-60 coagulation factor II, thrombin Homo sapiens 34-42 7749863-2 1995 In platelets stimulated by 1 U/mL thrombin, protein-tyrosine phosphorylation was maximal after 2 minutes and was followed by protein-tyrosine dephosphorylation. Tyrosine 133-141 coagulation factor II, thrombin Homo sapiens 34-42 7721957-2 1995 These proteins were divided in two groups: the first group included the 65-, 85-, 95-, and 120-kDa bands, which were tyrosine phosphorylated also in thrombin-stimulated platelets; the second group (80-, 135-, and 150-kDa bands) included proteins whose tyrosine phosphorylation was exclusively promoted by Con A, but not by thrombin. Tyrosine 117-125 coagulation factor II, thrombin Homo sapiens 149-157 7990127-7 1994 Several of the peptides were synthesized chemically and one of the peptides was shown to antagonise platelet aggregation triggered by the agonist peptide, and to inhibit serotonin release and tyrosine phosphorylation triggered by either thrombin or the agonist peptide. Tyrosine 192-200 coagulation factor II, thrombin Homo sapiens 237-245 7806502-2 1994 We have found that activated p72syk, which is phosphorylated on tyrosine residue(s), translocates from the Triton X-100-soluble fraction to the Triton X-100-insoluble, cytoskeleton-rich fraction after thrombin stimulation. Tyrosine 64-72 coagulation factor II, thrombin Homo sapiens 201-209 7798249-4 1994 Incubation of washed platelets for 24 h with herbimycin A (5 microM) abolished the thrombin-stimulated cytoskeletal enzyme activity of pp60c-src in parallel with a reduction in the tyrosine phosphorylation of multiple platelet proteins, as assessed with anti-phosphotyrosine immunoblots. Tyrosine 181-189 coagulation factor II, thrombin Homo sapiens 83-91 7947765-9 1994 The tyrosine of the peptide is sandwiched between the thrombin aromatic rings and is most likely the prime source of the specificity of the thrombomodulin-thrombin interaction. Tyrosine 4-12 coagulation factor II, thrombin Homo sapiens 54-62 7947765-9 1994 The tyrosine of the peptide is sandwiched between the thrombin aromatic rings and is most likely the prime source of the specificity of the thrombomodulin-thrombin interaction. Tyrosine 4-12 coagulation factor II, thrombin Homo sapiens 155-163 7524356-4 1994 The thrombin receptor, which belongs to the guanine nucleotide binding protein (G protein)-coupled receptor family, has recently been shown to induce rapid tyrosine phosphorylation of cellular proteins. Tyrosine 156-164 coagulation factor II, thrombin Homo sapiens 4-12 7980464-8 1994 Using synthetic peptides that bind to anion-binding site I of thrombin (thrombin-receptor sequence 52-66 and hirudin sequence 54-65 SO4 Tyr), we found that the rate of thrombin-catalysed activation of wt-HPC in the presence of calcium could be increased severalfold in a dose-dependent manner. Tyrosine 136-139 coagulation factor II, thrombin Homo sapiens 62-70 7925997-1 1994 We show the presence of the tyrosine kinase JAK2 in human platelets and demonstrate that it undergoes phosphorylation on tyrosine residues on challenge with the G protein receptor stimulus, thrombin, or the tyrosine phosphatase inhibitor, peroxovanadate. Tyrosine 28-36 coagulation factor II, thrombin Homo sapiens 190-198 7925997-3 1994 The protein kinase C (PKC) inhibitor, Ro 31-8220, and intracellular Ca2+ chelator, BAPTA-AM, also inhibit thrombin-induced phosphorylation of JAK2, while the phorbol ester, phorbol dibutyrate (PDBu), and Ca2+ ionophore, A23187, induce tyrosine phosphorylation of JAK2. Tyrosine 235-243 coagulation factor II, thrombin Homo sapiens 106-114 7925997-4 1994 These results suggest that tyrosine phosphorylation of JAK2 stimulated by thrombin may be mediated downstream of phosphoinositide metabolism. Tyrosine 27-35 coagulation factor II, thrombin Homo sapiens 74-82 7961845-5 1994 Specifically, thrombin induced the tyrosine phosphorylation and activation of pp72syk independent of platelet aggregation. Tyrosine 35-43 coagulation factor II, thrombin Homo sapiens 14-22 7524356-5 1994 In the present study, we investigated the role of protein-tyrosine phosphorylation in mediating the cellular responses elicited by thrombin in human glomerular mesangial cells. Tyrosine 58-66 coagulation factor II, thrombin Homo sapiens 131-139 7524356-6 1994 Amino acid labeling followed by immunoprecipitation with phosphotyrosine antibodies demonstrate that thrombin stimulates tyrosine phosphorylation of a set of cellular proteins. Tyrosine 64-72 coagulation factor II, thrombin Homo sapiens 101-109 8060352-0 1994 Thrombin-induced Ca2+ influx and protein tyrosine phosphorylation in endothelial cells is inhibited by herbimycin A. Tyrosine 41-49 coagulation factor II, thrombin Homo sapiens 0-8 8060352-4 1994 alpha-Thrombin caused significant tyrosine phosphorylation of mainly two proteins and evoked typical biphasic changes of free cytosolic Ca2+ concentration. Tyrosine 34-42 coagulation factor II, thrombin Homo sapiens 6-14 8060352-5 1994 We show that 24 h pretreatment with herbimycin A inhibited alpha-thrombin-induced endothelial protein tyrosine phosphorylation. Tyrosine 102-110 coagulation factor II, thrombin Homo sapiens 65-73 8060352-7 1994 The data suggest that protein tyrosine phosphorylation by alpha-thrombin is involved in the regulation of alpha-thrombin-induced Ca2+ influx into endothelial cells. Tyrosine 30-38 coagulation factor II, thrombin Homo sapiens 64-72 8060352-7 1994 The data suggest that protein tyrosine phosphorylation by alpha-thrombin is involved in the regulation of alpha-thrombin-induced Ca2+ influx into endothelial cells. Tyrosine 30-38 coagulation factor II, thrombin Homo sapiens 112-120 8119999-6 1994 The crucial structural elements of this sequence necessary for thrombin binding appear to be a cluster of negatively charged residues as well as three tyrosine residues that, in the native protein, may be sulfated. Tyrosine 151-159 coagulation factor II, thrombin Homo sapiens 63-71 8199204-2 1994 Thrombin (1 U/ml) elicited marked protein-tyrosine phosphorylation, PKC activation, and PtdIns(3,4)P2 synthesis. Tyrosine 42-50 coagulation factor II, thrombin Homo sapiens 0-8 8199204-5 1994 Both staurosporine and tyrphostin inhibited thrombin-stimulated tyrosine phosphorylation and PtdIns(3,4)P2 synthesis. Tyrosine 64-72 coagulation factor II, thrombin Homo sapiens 44-52 8023898-7 1994 Lipopeptides also stimulated tyrosine phosphorylation of several proteins with molecular masses similar to those found to be tyrosine phosphorylated in response to thrombin, and Pam3CysSer(Lys)4 led to an increase in the cytosolic calcium concentration. Tyrosine 29-37 coagulation factor II, thrombin Homo sapiens 164-172 8023898-7 1994 Lipopeptides also stimulated tyrosine phosphorylation of several proteins with molecular masses similar to those found to be tyrosine phosphorylated in response to thrombin, and Pam3CysSer(Lys)4 led to an increase in the cytosolic calcium concentration. Tyrosine 125-133 coagulation factor II, thrombin Homo sapiens 164-172 7526916-3 1994 To further investigate whether tyrosine phosphorylation is a step in the pathway of activation of PLD in response to thrombin, we tested the effects of a series of PTK inhibitors on the activity of platelet PLD. Tyrosine 31-39 coagulation factor II, thrombin Homo sapiens 117-125 7515012-2 1994 We found that chelation of extracellular calcium completely prevented phosphorylation of tyrosine proteins induced by thapsigargin and phorbol 12-myristate 13-acetate (PMA), whereas, when induced by thrombin, it prevented a subset of tyrosine proteins. Tyrosine 234-242 coagulation factor II, thrombin Homo sapiens 199-207 8188664-4 1994 Stimulation of platelets with 0.1 unit/ml thrombin resulted in a significant increase (2-5-fold) of the tyrosine phosphorylation of the PDGFR-alpha, as determined by immunoprecipitation with phosphotyrosine antiserum as well as with PDGFR-alpha antiserum. Tyrosine 104-112 coagulation factor II, thrombin Homo sapiens 42-50 7510689-4 1994 Thrombin stimulated tyrosine phosphorylation of several proteins of molecular mass 40, 42, 70, 120, and 130 kDa. Tyrosine 20-28 coagulation factor II, thrombin Homo sapiens 0-8 7510689-5 1994 There was a good correlation between thrombin-induced tyrosine phosphorylation of the latter three proteins and Ca2+ mobilization. Tyrosine 54-62 coagulation factor II, thrombin Homo sapiens 37-45 7917785-2 1994 Preincubation of platelets with the tyrosine phosphatase inhibitor orthovanadate prevented the tyrosine dephosphorylation of P38 and P140, and reduced platelet aggregation induced by thrombin receptor activation. Tyrosine 36-44 coagulation factor II, thrombin Homo sapiens 183-191 7917785-0 1994 Integrin-dependent protein dephosphorylation on tyrosine induced by activation of the thrombin receptor in human platelets. Tyrosine 48-56 coagulation factor II, thrombin Homo sapiens 86-94 7917785-1 1994 Activation of human platelets by thrombin or a thrombin receptor-activating peptide (TRAP) resulted in a decrease in tyrosine phosphorylation of two proteins, P38 and P140. Tyrosine 117-125 coagulation factor II, thrombin Homo sapiens 33-41 8297360-0 1994 Tyrosine phosphorylation and activation of mitogen-activated protein kinases by thrombin in human platelets: possible involvement in late arachidonic acid release. Tyrosine 0-8 coagulation factor II, thrombin Homo sapiens 80-88 7917785-1 1994 Activation of human platelets by thrombin or a thrombin receptor-activating peptide (TRAP) resulted in a decrease in tyrosine phosphorylation of two proteins, P38 and P140. Tyrosine 117-125 coagulation factor II, thrombin Homo sapiens 47-55 8297360-1 1994 Thrombin induced tyrosine phosphorylation of 41-kDa and 43-kDa mitogen-activated protein (MAP) kinases in human platelets which was detectable at 1 min and peaked at 2 min after thrombin stimulation as assessed by immunoprecipitation and immunoblotting. Tyrosine 17-25 coagulation factor II, thrombin Homo sapiens 0-8 8297360-1 1994 Thrombin induced tyrosine phosphorylation of 41-kDa and 43-kDa mitogen-activated protein (MAP) kinases in human platelets which was detectable at 1 min and peaked at 2 min after thrombin stimulation as assessed by immunoprecipitation and immunoblotting. Tyrosine 17-25 coagulation factor II, thrombin Homo sapiens 178-186 7691054-1 1993 When intracellular Ca2+ pools are released during platelet stimulation by thrombin, elevation of platelet cytosolic Ca2+ concentration induces tyrosine phosphorylation of a 130 kDa protein, and refilling the pools mediates dephosphorylation of this protein [Vostal, Jackson and Shulman (1991) J. Biol. Tyrosine 143-151 coagulation factor II, thrombin Homo sapiens 74-82 8303657-5 1993 We find that replacement by Leu of Val and by Tyr of Phe adjacent to Gln is minimally tolerated and the resulting peptides are partially effective in selectively blocking thrombin-induced platelet aggregation. Tyrosine 46-49 coagulation factor II, thrombin Homo sapiens 171-179 7689859-0 1993 Thrombin activation of human platelets causes tyrosine phosphorylation of PLC-gamma 2. Tyrosine 46-54 coagulation factor II, thrombin Homo sapiens 0-8 7689859-3 1993 Stimulation of platelets with the potent agonist, thrombin, led to a rapid and transient phosphorylation of PLC-gamma 2 on tyrosine residues. Tyrosine 123-131 coagulation factor II, thrombin Homo sapiens 50-58 7689831-0 1993 Thrombin and thrombin receptor agonist peptide induce tyrosine phosphorylation and tyrosine kinases in the platelet cytoskeleton. Tyrosine 54-62 coagulation factor II, thrombin Homo sapiens 0-8 7689831-0 1993 Thrombin and thrombin receptor agonist peptide induce tyrosine phosphorylation and tyrosine kinases in the platelet cytoskeleton. Tyrosine 54-62 coagulation factor II, thrombin Homo sapiens 13-21 7686553-1 1993 Tyrosine phosphorylation of multiple platelet proteins is stimulated by thrombin and other agonists that cause platelet aggregation and secretion. Tyrosine 0-8 coagulation factor II, thrombin Homo sapiens 72-80 8394081-9 1993 Protein tyrosine phosphorylation induced by thrombin was not affected by genistein, suggesting that the inhibitory effect of genistein on polyphosphoinositides was unrelated to tyrosine kinase inhibition. Tyrosine 8-16 coagulation factor II, thrombin Homo sapiens 44-52 7687438-3 1993 While both 115 and 75 kDa tyrosine-phosphorylated protein bands were quickly dephosphorylated in normal platelets, thrombin-induced 115 kDa or STA2-induced 115 and 75 kDa protein bands were not dephosphorylated in thrombasthenic platelets. Tyrosine 26-34 coagulation factor II, thrombin Homo sapiens 115-123 7686553-7 1993 Tyrosine phosphorylation of the 50-68-kD and 140-kD proteins was also observed when (a) fibrinogen binding was stimulated by agonists such as epinephrine, ADP, or thrombin instead of by anti-LIBS6; (b) fragment X, a dimeric plasmin-derived fragment of fibrinogen was used instead of fibrinogen; or (c) alpha IIb beta 3 complexes were cross-linked by antibodies, even in the absence of fibrinogen. Tyrosine 0-8 coagulation factor II, thrombin Homo sapiens 163-171 1385445-3 1992 We show that thrombin and collagen activation of platelets causes an induction of tyrosine phosphorylation of pp125FAK and that pp125FAK molecules isolated from activated platelets display enhanced levels of phosphorylation in immune-complex kinase assays. Tyrosine 82-90 coagulation factor II, thrombin Homo sapiens 13-21 8391259-7 1993 Our results provide the first evidence for a direct or indirect regulation of PtdIns(3,4)P2 accumulation and PLC gamma 1 activity by tyrosine phosphorylation during thrombin stimulation of human platelets. Tyrosine 133-141 coagulation factor II, thrombin Homo sapiens 165-173 8428900-1 1993 Activation of platelets by thrombin results in a dramatic increase in tyrosine phosphorylation on multiple cellular proteins (Ferrell, J. E., and Martin, G. S. (1988) Mol. Tyrosine 70-78 coagulation factor II, thrombin Homo sapiens 27-35 8428900-21 1993 These results indicate that p72syk can be a responsible enzyme to the protein-tyrosine phosphorylation events following the platelet activation by thrombin and may be negatively regulated by Ca2+ in a calmodulin-dependent manner, inter alia myosin light-chain kinase, in thrombin-stimulated platelets. Tyrosine 78-86 coagulation factor II, thrombin Homo sapiens 147-155 8428900-21 1993 These results indicate that p72syk can be a responsible enzyme to the protein-tyrosine phosphorylation events following the platelet activation by thrombin and may be negatively regulated by Ca2+ in a calmodulin-dependent manner, inter alia myosin light-chain kinase, in thrombin-stimulated platelets. Tyrosine 78-86 coagulation factor II, thrombin Homo sapiens 271-279 8422913-0 1993 The tyrosine kinase inhibitors methyl 2,5-dihydroxycinnamate and genistein reduce thrombin-evoked tyrosine phosphorylation and Ca2+ entry in human platelets. Tyrosine 4-12 coagulation factor II, thrombin Homo sapiens 82-90 8422913-3 1993 Here we have investigated the effects of two tyrosine kinase inhibitors, methyl 2,5-dihydroxycinnamate and genistein, on thrombin-evoked protein tyrosine phosphorylation and Ca2+ signal generation in fura-2-loaded human platelets. Tyrosine 45-53 coagulation factor II, thrombin Homo sapiens 121-129 8422913-4 1993 Both compounds inhibited thrombin-evoked tyrosine phosphorylation and reduced the elevation of [Ca2+]i in the presence, but not the absence, of external Ca2+. Tyrosine 41-49 coagulation factor II, thrombin Homo sapiens 25-33 8422913-8 1993 These data are compatible with a role for protein tyrosine phosphorylation contributing to thrombin-evoked Ca2+ entry in human platelets. Tyrosine 50-58 coagulation factor II, thrombin Homo sapiens 91-99 1281303-2 1992 Numerous proteins were found to be phosphorylated on tyrosine in response to thrombin-induced platelet activation and aggregation. Tyrosine 53-61 coagulation factor II, thrombin Homo sapiens 77-85 7681669-1 1993 Both thrombin and collagen induced the phosphorylation of tyrosine in numerous proteins in platelets, with collagen causing the phosphorylation of an additional 40 kDa protein. Tyrosine 58-66 coagulation factor II, thrombin Homo sapiens 5-13 8381415-7 1993 In EGF4, which is essential for protein C activation by the thrombin-TM complex, critical residues were: Asp-349, Glu-357, Tyr-358, and Phe-376. Tyrosine 123-126 coagulation factor II, thrombin Homo sapiens 60-68 8381415-8 1993 In EGF5-EGF6, critical residues within a proposed acidic thrombin-binding region were: Glu-408, Tyr-413, Ile-414, Leu-415, Asp-416, Asp-417, Asp-423, Ile-424, Asp-425, and Glu-426. Tyrosine 96-99 coagulation factor II, thrombin Homo sapiens 57-65 1385445-7 1992 The induction of tyrosine phosphorylation of pp125FAK was inhibited in thrombin- and collagen-treated platelets preincubated with cytochalasin D, which prevents actin polymerization following activation. Tyrosine 17-25 coagulation factor II, thrombin Homo sapiens 71-79 1511739-3 1992 The increase of protein-tyrosine phosphorylation bands (135-, 124-, 76-, 64-, and 60-kDa) induced with thrombin was also inhibited by these inhibitors in a dose-dependent manner. Tyrosine 24-32 coagulation factor II, thrombin Homo sapiens 103-111 1382425-2 1992 The inhibition of thrombin-induced aggregation correlates with their potency in inhibiting phosphorylation of proteins on tyrosine residues. Tyrosine 122-130 coagulation factor II, thrombin Homo sapiens 18-26 1382425-8 1992 These results suggest that thrombin induces the phosphorylation of proteins on tyrosine residues which most probably results in the activation of phosphoinositide kinases. Tyrosine 79-87 coagulation factor II, thrombin Homo sapiens 27-35 1511739-4 1992 These inhibitors also blocked the platelet aggregation and protein-tyrosine phosphorylation induced with thrombin in aspirin-treated platelets. Tyrosine 67-75 coagulation factor II, thrombin Homo sapiens 105-113 1544934-0 1992 Inhibition of tyrosine phosphorylation prevents thrombin-induced mitogenesis, but not intracellular free calcium release, in vascular smooth muscle cells. Tyrosine 14-22 coagulation factor II, thrombin Homo sapiens 48-56 1323853-5 1992 Although thrombin stimulation had no effect on the level of phosphorylation of phospholipase C-gamma 1 and only slightly increased the tyrosine phosphorylation of rasGAP, the agonist induced the association of rasGAP with rap1B, as indicated by the appearance of rap1B on a Western blot of rasGAP immunoprecipitates. Tyrosine 135-143 coagulation factor II, thrombin Homo sapiens 9-17 1324667-5 1992 Protein-tyrosine phosphorylation, which is reportedly involved in generation of 3-phosphorylated phosphoinositides, was elicited in thrombin-activated platelets. Tyrosine 8-16 coagulation factor II, thrombin Homo sapiens 132-140 1554716-6 1992 Interestingly, all sites of tyrosine sulfation border thrombin cleavage sites. Tyrosine 28-36 coagulation factor II, thrombin Homo sapiens 54-62 1387329-0 1992 Alterations in thrombin-induced protein tyrosine phosphorylation of platelets from patients with chronic myelogenous leukemia. Tyrosine 40-48 coagulation factor II, thrombin Homo sapiens 15-23 1387329-2 1992 We studied thrombin-induced tyrosine-specific protein phosphorylation in normal platelets and those from patients with chronic myelogenous leukemia (CML) and other myeloproliferative disorders (MPD) using immunoblotting with antiphosphotyrosine (anti-P-Tyr) antibody. Tyrosine 28-36 coagulation factor II, thrombin Homo sapiens 11-19 1387329-5 1992 Thrombin enhanced the tyrosine phosphorylation of p120 and p60, not only in normal platelets, but also in CML platelets, although the response was more delayed and the duration was shorter in CML platelets than those in normal platelets. Tyrosine 22-30 coagulation factor II, thrombin Homo sapiens 0-8 1387329-7 1992 These results suggest that the tyrosine phosphorylation of p36, which was probably identical to lipocortin, inhibits thrombin-induced platelet aggregation through anti-phospholipase A2 (anti-PLA2) activity. Tyrosine 31-39 coagulation factor II, thrombin Homo sapiens 117-125 1547237-6 1992 The global folding of these conformations is similar to that in the thrombin-bound state, as indicated by NOE"s involving the side-chain protons of residues Phe(56), Ile(59), Pro(60), Tyr(63), and Leu(64). Tyrosine 184-187 coagulation factor II, thrombin Homo sapiens 68-76 1544885-1 1992 Activation of platelets by thrombin and other physiological agonists leads to a dramatic increase in tyrosine phosphorylation of multiple cellular proteins (Ferrell, J. E., and Martin, G. S. (1988) Mol. Tyrosine 101-109 coagulation factor II, thrombin Homo sapiens 27-35 1544885-14 1992 We show that GAP and two proteins of 59 and 68 kDa are phosphorylated on tyrosine after thrombin stimulation and that three Src-related protein tyrosine kinases, Fyn, Lyn and Yes, are associated with GAP in complexes, detectable only after agonist stimulation. Tyrosine 73-81 coagulation factor II, thrombin Homo sapiens 88-96 1544934-4 1992 Herbimycin A, a specific tyrosine kinase inhibitor, both decreases thrombin-induced mitogenesis by greater than 90% and abolishes tyrosine phosphorylation of phospholipase C (PLC)-gamma-1. Tyrosine 25-33 coagulation factor II, thrombin Homo sapiens 67-75 1544934-7 1992 Furthermore, tyrosine phosphorylation is a necessary step in thrombin"s mitogenic signal transduction cascade, but it is not essential for thrombin-induced release of calcium from intracellular stores. Tyrosine 13-21 coagulation factor II, thrombin Homo sapiens 61-69 1372480-0 1992 Inhibition by sodium nitroprusside or PGE1 of tyrosine phosphorylation induced in platelets by thrombin or ADP. Tyrosine 46-54 coagulation factor II, thrombin Homo sapiens 95-103 1372480-4 1992 Tyrosine phosphorylation in platelets activated by alpha-thrombin (1 nM) was inhibited by prostaglandin (PG) E1 (2 microM) or by sodium nitroprusside (100 microM). Tyrosine 0-8 coagulation factor II, thrombin Homo sapiens 57-65 1372480-6 1992 On the other hand, a higher concentration of alpha-thrombin (10 nM) induced tyrosine phosphorylation of the same proteins, elevation of [Ca2+]i, platelet aggregation, and serotonin secretion, irrespective of pretreatment of platelets by either PGE1 or sodium nitroprusside. Tyrosine 76-84 coagulation factor II, thrombin Homo sapiens 51-59 1372480-7 1992 Inhibition by sodium nitroprusside of tyrosine phosphorylation induced by alpha-thrombin (1 nM) was accompanied by an increased concentration of cGMP. Tyrosine 38-46 coagulation factor II, thrombin Homo sapiens 80-88 1372480-10 1992 Thus tyrosine phosphorylation in platelets can be inhibited by elevation of either cAMP or cGMP, an effect that is overcome by a high concentration of thrombin, resulting in granule secretion and aggregation. Tyrosine 5-13 coagulation factor II, thrombin Homo sapiens 151-159 1939071-5 1991 Both inhibitors bind to thrombin in a similar way as to trypsin, but thrombin has an extra loop, the "Tyr-Pro-Pro-Trp loop," not present in trypsin, which gives further binding interactions and is seen to move somewhat to accommodate binding of the different inhibitors. Tyrosine 102-105 coagulation factor II, thrombin Homo sapiens 69-77 2017148-3 1991 Although thrombin induced an increase in the platelet phosphotyrosine content, genistein at 100 micrograms/ml only slightly attenuated thrombin-induced protein tyrosine phosphorylation. Tyrosine 61-69 coagulation factor II, thrombin Homo sapiens 9-17 1832159-2 1991 We found that the Ca2+ ionophore, A23187, and thrombin elevate cytosolic calcium ([Ca2+]i) equally and cause tyrosine phosphorylation of a 130-kDa protein and to a lesser extent 80- and 60-kDa proteins. Tyrosine 109-117 coagulation factor II, thrombin Homo sapiens 46-54 1832159-3 1991 Chelation of [Ca2+]i by 1,2-bis(2-aminophenoxyethane)-N,N,N",N"-tetraacetic acid/acetomethoxy ester decreased thrombin-induced tyrosine phosphorylation responses. Tyrosine 127-135 coagulation factor II, thrombin Homo sapiens 110-118 1832159-5 1991 Tyrosine phosphorylation persisted in the presence or absence of extracellular calcium after thrombin stimulation but subsided rapidly after A23187 addition if extracellular calcium was present. Tyrosine 0-8 coagulation factor II, thrombin Homo sapiens 93-101 1715582-1 1991 Activation of platelets with thrombin and other agonists causes a rapid increase in the phosphorylation of multiple proteins on tyrosine. Tyrosine 128-136 coagulation factor II, thrombin Homo sapiens 29-37 1985956-4 1991 Kinetic analysis of these mutant proteins and synthetic peptide fragments and available structural information on thrombin and hirudin derived from protein crystallography and two-dimensional NMR studies indicate that the amino-terminal region of hirudin binds at the apolar binding/active site region of thrombin, with Tyr-3 occupying the S3 specificity site. Tyrosine 320-323 coagulation factor II, thrombin Homo sapiens 114-122 1996982-5 1991 Considering the previous report from our laboratory that thrombin and collagen induced tyrosine phosphorylation in 135-, 124- and 76-kDa proteins (Nakamura, S. and Yamamura, H. (1989) J. Biol. Tyrosine 87-95 coagulation factor II, thrombin Homo sapiens 57-65 1991108-2 1991 We demonstrate that nitration or iodination of Tyr-63 restores hirudin-thrombin affinity to levels similar to or exceeding that of the natural inhibitor. Tyrosine 47-50 coagulation factor II, thrombin Homo sapiens 71-79 1991108-3 1991 In contrast, nitration of Tyr-3 reduces the affinity of hirudin for thrombin. Tyrosine 26-29 coagulation factor II, thrombin Homo sapiens 68-76 1991108-5 1991 The mechanism of the observed changes in thrombin affinity may involve a reduction in the pK of the hydroxyl group of tyrosine due to substitution of the electrophilic iodo or nitro group on the phenyl ring, resulting in an increased negative charge at neutral pH. Tyrosine 118-126 coagulation factor II, thrombin Homo sapiens 41-49 1991108-6 1991 For Tyr-63, this effect mimics the sulfatotyrosine of natural hirudin, leading to an increased thrombin affinity at the anion-binding exosite. Tyrosine 4-7 coagulation factor II, thrombin Homo sapiens 95-103 1991108-7 1991 For Tyr-3, the increased polarity may destabilize its interaction within the apolar-binding site of thrombin. Tyrosine 4-7 coagulation factor II, thrombin Homo sapiens 100-108 1991108-8 1991 Substitution of the highly conserved Tyr-3 residue with Phe or Trp not only enables specific and quantitative chemical modification at Tyr-63 but also independently increases hirudin-thrombin affinity. Tyrosine 37-40 coagulation factor II, thrombin Homo sapiens 183-191 2133252-4 1990 Using immunoblots of platelet proteins revealed with an anti-phosphotyrosine antibody, tyrphostins were effective inhibitors of tyrosine phosphorylation elicited by thrombin. Tyrosine 68-76 coagulation factor II, thrombin Homo sapiens 165-173 1719348-3 1991 For example, a 95-kDa thrombin-induced P-Tyr protein in platelets is recognized by these polyclonal antibodies but reacts poorly with other types of anti-P-Tyr antibodies (A. Tyrosine 41-44 coagulation factor II, thrombin Homo sapiens 22-30 1988465-3 1991 The most potent adhesive thrombin analogue (NO2-alpha-thrombin) was obtained by nitration of tyrosine residues. Tyrosine 93-101 coagulation factor II, thrombin Homo sapiens 25-33 1988465-3 1991 The most potent adhesive thrombin analogue (NO2-alpha-thrombin) was obtained by nitration of tyrosine residues. Tyrosine 93-101 coagulation factor II, thrombin Homo sapiens 54-62 1702789-1 1990 Treatment of platelets with thrombin was shown previously to induce rapid changes in tyrosine phosphorylation of several platelet proteins. Tyrosine 85-93 coagulation factor II, thrombin Homo sapiens 28-36 2369893-9 1990 Furthermore hirudin binds with its N-terminal three residues Val, Val, Tyr to the thrombin active site cleft. Tyrosine 71-74 coagulation factor II, thrombin Homo sapiens 82-90 1698364-0 1990 Elevation of cAMP, but not cGMP, inhibits thrombin-stimulated tyrosine phosphorylation in human platelets. Tyrosine 62-70 coagulation factor II, thrombin Homo sapiens 42-50 1698364-2 1990 The stimulation of tyrosine phosphorylation elicited by thrombin can be completely inhibited by preincubation with 10nm prostacyclin (PGI2), 1 microM PGD2, or 1mM N2,2"-O-dibutyryl-cAMP. Tyrosine 19-27 coagulation factor II, thrombin Homo sapiens 56-64 11557600-9 2001 After TNF-alpha exposure, thrombin caused increased tyrosine phosphorylation of junctional proteins and actin stress fiber formation as well as augmented endothelial permeability. Tyrosine 52-60 coagulation factor II, thrombin Homo sapiens 26-34 2160076-7 1990 Binding of peptide 105Y (Ser-Ile-Pro-Pro-Glu-Val-Lys-Phe-Asn-Lys-Pro-Phe-Val-Tyr-Leu-Ile) is blocked by alpha 1-AT-elastase complexes, antithrombin III (AT III)-thrombin complexes, alpha 1-antichymotrypsin (alpha 1-ACT)-cathepsin G complexes, and, to a lesser extent, complement component C1 inhibitor-C1s complexes, but not by the corresponding native proteins. Tyrosine 77-80 coagulation factor II, thrombin Homo sapiens 139-147 2294999-1 1990 A synthetic, tyrosine-sulfated, dodecapeptide (BG8865) modeled on residues 53-64 of hirudin was found to elevate the activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT) of human plasma in a dose-dependent manner. Tyrosine 13-21 coagulation factor II, thrombin Homo sapiens 166-174