PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 33080786-7 2020 The Cx43CT residues involved in the CaM interaction overlap with tyrosines phosphorylated by Pyk2 and Src. Tyrosine 65-74 protein tyrosine kinase 2 beta Homo sapiens 93-97 33277387-5 2021 We showed that MCP-1 induces Src phosphorylation in a similar time frame and that the MCP-1-induced Pyk2 tyrosine phosphorylation is controlled by the Src family kinase. Tyrosine 105-113 protein tyrosine kinase 2 beta Homo sapiens 100-104 2736256-2 1989 The two PTKs phosphorylated poly(Glu: Tyr; 4:1) very effectively with apparent Km values of 0.3 mg/ml for insulin receptor PTK and 0.8 mg/ml for lung PTK. Tyrosine 38-41 protein tyrosine kinase 2 beta Homo sapiens 8-11 32727877-0 2020 Pyk2 Regulates Human Papillomavirus Replication by Tyrosine Phosphorylation of the E2 Protein. Tyrosine 51-59 protein tyrosine kinase 2 beta Homo sapiens 0-4 32727877-2 2020 In this study, we demonstrate that the non-receptor tyrosine kinase Pyk2 phosphorylates tyrosine 131 in the E2 transactivation domain. Tyrosine 52-60 protein tyrosine kinase 2 beta Homo sapiens 68-72 32727877-7 2020 The present study demonstrates that the Pyk2 tyrosine kinase phosphorylates E2 at tyrosine 131, interfering with genome replication. Tyrosine 45-53 protein tyrosine kinase 2 beta Homo sapiens 40-44 31755225-1 2020 A new class of pyrimidine derivatives were identified as potent protein tyrosine kinase (PTK) inhibitors for the treatment of idiopathic pulmonary fibrosis (IPF). Tyrosine 72-80 protein tyrosine kinase 2 beta Homo sapiens 89-92 32510326-6 2020 Phosphorylation of the FAK family member Pyk2 at tyrosine 402 is decreased in NK92 CD56-KO cells, demonstrating a functional link between CD56 and signaling in human NK cells. Tyrosine 49-57 protein tyrosine kinase 2 beta Homo sapiens 41-45 29133485-3 2018 Pyk2 colocalizes with cortactin to invadopodia of invasive breast cancer cells, where it mediates epidermal growth factor-induced cortactin tyrosine phosphorylation both directly and indirectly via Src-mediated Abl-related gene (Arg) activation, leading to actin polymerization in invadopodia, extracellular matrix degradation, and tumor cell invasion. Tyrosine 140-148 protein tyrosine kinase 2 beta Homo sapiens 0-4 30250159-6 2018 We further show that PYK2 enhances the tyrosine phosphorylation of both TAZ and LATS1/2 and concomitantly TAZ stability, and that PYK2 protein level correlates with the level of TAZ protein in primary breast tumors. Tyrosine 39-47 protein tyrosine kinase 2 beta Homo sapiens 21-25 29438996-6 2018 Cell-based analyses of tyrosine kinase, PYK2, revealed that SUMOylation at four lysine residues promoted PYK2 autophosphorylation at tyrosine 402, which in turn enhanced its interaction with SRC and full activation of the SRC-PYK2 complex. Tyrosine 23-31 protein tyrosine kinase 2 beta Homo sapiens 105-109 29438996-6 2018 Cell-based analyses of tyrosine kinase, PYK2, revealed that SUMOylation at four lysine residues promoted PYK2 autophosphorylation at tyrosine 402, which in turn enhanced its interaction with SRC and full activation of the SRC-PYK2 complex. Tyrosine 23-31 protein tyrosine kinase 2 beta Homo sapiens 105-109 31671564-4 2019 We searched for additional mutations in the putative secondary driver gene protein tyrosine kinase 2 beta (PTK2B) and we developed new mutational signatures that explain the mutational pattern observed in UM. Tyrosine 83-91 protein tyrosine kinase 2 beta Homo sapiens 107-112 27271350-2 2016 The supramolecular complexation of the Tyr residues efficiently precludes their subsequent PTK-catalysed phosphorylation. Tyrosine 39-42 protein tyrosine kinase 2 beta Homo sapiens 91-94 28818394-4 2017 Tyrosine phosphorylation of focal adhesion kinase (FAK) family members FAK and PYK2 was significantly reduced by dasatinib, and select inhibitors for these kinases PF431396, which inhibits both FAK and PYK2, and PF573228, which only inhibits FAK and not PYK2, significantly reduced matrix contraction by transdifferentiated Muller cells. Tyrosine 0-8 protein tyrosine kinase 2 beta Homo sapiens 79-83 28818394-4 2017 Tyrosine phosphorylation of focal adhesion kinase (FAK) family members FAK and PYK2 was significantly reduced by dasatinib, and select inhibitors for these kinases PF431396, which inhibits both FAK and PYK2, and PF573228, which only inhibits FAK and not PYK2, significantly reduced matrix contraction by transdifferentiated Muller cells. Tyrosine 0-8 protein tyrosine kinase 2 beta Homo sapiens 202-206 28818394-4 2017 Tyrosine phosphorylation of focal adhesion kinase (FAK) family members FAK and PYK2 was significantly reduced by dasatinib, and select inhibitors for these kinases PF431396, which inhibits both FAK and PYK2, and PF573228, which only inhibits FAK and not PYK2, significantly reduced matrix contraction by transdifferentiated Muller cells. Tyrosine 0-8 protein tyrosine kinase 2 beta Homo sapiens 202-206 28385807-4 2017 Pyk2-induced tyrosine phosphorylation of VE-PTP promoted Src binding to VE-PTP, Src activation, and subsequent VE-cadherin phosphorylation and thereby increased the endothelial permeability response. Tyrosine 13-21 protein tyrosine kinase 2 beta Homo sapiens 0-4 28385807-8 2017 Thus Pyk2 activation secondary to STIM1-induced SOCE causes tyrosine phosphorylation of VE-PTP, and VE-PTP, in turn, binds to and activates Src, thereby phosphorylating VE-cadherin to increase endothelial permeability through disassembly of AJs. Tyrosine 60-68 protein tyrosine kinase 2 beta Homo sapiens 5-9 25713079-4 2015 Pyk2 was identified as the major tyrosine-phosphorylated protein in response to membrane depolarization. Tyrosine 33-41 protein tyrosine kinase 2 beta Homo sapiens 0-4 26743491-5 2016 Combined imaging and Western blot studies showed that osteoprotegerin induced dephosphorylation of Tyr 402 in Pyk2 and decreased its labeling in peripheral adhesion regions. Tyrosine 99-102 protein tyrosine kinase 2 beta Homo sapiens 110-114 26743491-6 2016 osteoprotegerin induced increased intracellular labeling of Tyr 402 in Pyk2, Tyr 416 in Src, increased dephosphorylation of Tyr 527 in Src, and increased Pyk2/Src association in the central region of osteoclasts. Tyrosine 60-63 protein tyrosine kinase 2 beta Homo sapiens 71-75 26702053-8 2016 Treatment with FSH and increasing concentrations of exogenous IGF-1 triggers synergistic IRS1 tyrosine phosphorylation at PI3K-activating residues that persists downstream through protein kinase B (AKT) and FOXO1 (forkhead box protein O1) to drive synergistic expression of genes that underlies follicle maturation. Tyrosine 94-102 protein tyrosine kinase 2 beta Homo sapiens 180-196 26645934-4 2016 Mutating tyrosines at positions 625 and 627 dramatically reduced responses to Src or Pyk2 without altering the activation by PKA, suggesting they may contribute to CFTR regulation. Tyrosine 9-18 protein tyrosine kinase 2 beta Homo sapiens 85-89 25708150-2 2015 The functions of FAK and PYK2 are linked to autophosphorylation of their specific tyrosine residues, Tyr-397 in FAK and Tyr-402 in PYK2, and then association with different signalling proteins which mediate activation of downstream targets such as ERK and JNK mitogen-activated kinase cascades. Tyrosine 82-90 protein tyrosine kinase 2 beta Homo sapiens 25-29 25708150-2 2015 The functions of FAK and PYK2 are linked to autophosphorylation of their specific tyrosine residues, Tyr-397 in FAK and Tyr-402 in PYK2, and then association with different signalling proteins which mediate activation of downstream targets such as ERK and JNK mitogen-activated kinase cascades. Tyrosine 101-104 protein tyrosine kinase 2 beta Homo sapiens 25-29 25708150-2 2015 The functions of FAK and PYK2 are linked to autophosphorylation of their specific tyrosine residues, Tyr-397 in FAK and Tyr-402 in PYK2, and then association with different signalling proteins which mediate activation of downstream targets such as ERK and JNK mitogen-activated kinase cascades. Tyrosine 101-104 protein tyrosine kinase 2 beta Homo sapiens 131-135 25708150-2 2015 The functions of FAK and PYK2 are linked to autophosphorylation of their specific tyrosine residues, Tyr-397 in FAK and Tyr-402 in PYK2, and then association with different signalling proteins which mediate activation of downstream targets such as ERK and JNK mitogen-activated kinase cascades. Tyrosine 120-123 protein tyrosine kinase 2 beta Homo sapiens 25-29 25708150-2 2015 The functions of FAK and PYK2 are linked to autophosphorylation of their specific tyrosine residues, Tyr-397 in FAK and Tyr-402 in PYK2, and then association with different signalling proteins which mediate activation of downstream targets such as ERK and JNK mitogen-activated kinase cascades. Tyrosine 120-123 protein tyrosine kinase 2 beta Homo sapiens 131-135 25708150-5 2015 OGD induced primary down-regulation of FAK and PYK2 autophosphorylation (at Tyr 397 and Tyr 402, respectively) at 24-48 h of reoxygenation was accompanied by the diminution of phosphorylation/activation of Src and JNK. Tyrosine 76-79 protein tyrosine kinase 2 beta Homo sapiens 47-51 25708150-5 2015 OGD induced primary down-regulation of FAK and PYK2 autophosphorylation (at Tyr 397 and Tyr 402, respectively) at 24-48 h of reoxygenation was accompanied by the diminution of phosphorylation/activation of Src and JNK. Tyrosine 88-91 protein tyrosine kinase 2 beta Homo sapiens 47-51 25708150-8 2015 The inhibition of the early step of FAK and PYK2 activation demonstrated by the decrease of tyrosine autophosphorylation may comprise an important portion of the response expressed by modulation of some coupled signal transduction pathways. Tyrosine 92-100 protein tyrosine kinase 2 beta Homo sapiens 44-48 24965120-10 2014 Redox-sensitive kinase; PYK2 activation led to increased tyrosine phosphorylation of VE-cadherin and beta-catenin, leading to disruption of junctional assembly. Tyrosine 57-65 protein tyrosine kinase 2 beta Homo sapiens 24-28 24981431-0 2015 The upregulation of NR2A-containing N-methyl-D-aspartate receptor function by tyrosine phosphorylation of postsynaptic density 95 via facilitating Src/proline-rich tyrosine kinase 2 activation. Tyrosine 78-86 protein tyrosine kinase 2 beta Homo sapiens 151-181 24981431-10 2015 These results indicate that PSD-95 phosphorylation by Src facilitates the integration of Pyk2 to PSD-95 signal complex, the activation of Pyk2/Src, as well as the subsequent tyrosine phosphorylation of NR2A, which ultimately results in the upregulation of NMDA receptor function and synaptic transmission. Tyrosine 174-182 protein tyrosine kinase 2 beta Homo sapiens 89-93 24981431-10 2015 These results indicate that PSD-95 phosphorylation by Src facilitates the integration of Pyk2 to PSD-95 signal complex, the activation of Pyk2/Src, as well as the subsequent tyrosine phosphorylation of NR2A, which ultimately results in the upregulation of NMDA receptor function and synaptic transmission. Tyrosine 174-182 protein tyrosine kinase 2 beta Homo sapiens 138-142 25180269-7 2014 Experiments aimed to identify the kinase responsible for these observations revealed the presence of proline-rich tyrosine kinase 2 (PYK2), a focal adhesion kinase (FAK) family member, in human sperm, and the use of PF431396, an FAK inhibitor, supported the involvement of PYK2 in Tyr phosphorylation downstream of PKA activation. Tyrosine 281-284 protein tyrosine kinase 2 beta Homo sapiens 101-131 25180269-9 2014 Together, our observations support PYK2 as an intermediary component of Ca(2+) signaling between PKA-mediated and Tyr phosphorylations that is required for achieving functional human sperm capacitation. Tyrosine 114-117 protein tyrosine kinase 2 beta Homo sapiens 35-39 25143406-4 2014 When cells are stressed, Pyk2 and Pyk3 undergo increased autocatalytic tyrosine phosphorylation. Tyrosine 71-79 protein tyrosine kinase 2 beta Homo sapiens 25-29 24800979-0 2014 Adrenergic signaling regulates mitochondrial Ca2+ uptake through Pyk2-dependent tyrosine phosphorylation of the mitochondrial Ca2+ uniporter. Tyrosine 80-88 protein tyrosine kinase 2 beta Homo sapiens 65-69 24800979-8 2014 CONCLUSION: The alpha1-AR-Pyk2-dependent tyrosine phosphorylation of the MCU regulates mitochondrial Ca2+ entry and apoptosis in cardiac cells. Tyrosine 41-49 protein tyrosine kinase 2 beta Homo sapiens 26-30 23454381-4 2013 Here we show alpha1-adrenergic signaling inhibits beta-adrenergically activated Ca(2+) current, Ca(2+) transients and contractile force through phosphorylation of tyrosine residues in beta1-adrenoceptor by PTK. Tyrosine 163-171 protein tyrosine kinase 2 beta Homo sapiens 206-209 24025335-3 2013 Thrombin induced tyrosine phosphorylation of Pyk2, Gab1, and p115 RhoGEF, leading to Rac1- and RhoA-dependent Pak2 activation. Tyrosine 17-25 protein tyrosine kinase 2 beta Homo sapiens 45-49 22387535-10 2012 Biochanin inhibits NF-kappaB activation not only by blocking the upstream IKK, but also PTK that phosphorylate tyrosine residues of IkappaBalpha. Tyrosine 111-119 protein tyrosine kinase 2 beta Homo sapiens 88-91 22581436-0 2012 PYK-2 is tyrosine phosphorylated after activation of pituitary adenylate cyclase activating polypeptide receptors in lung cancer cells. Tyrosine 9-17 protein tyrosine kinase 2 beta Homo sapiens 0-5 22581436-4 2012 In this communication, the effects of PACAP on cytosolic Ca(2+) and PYK-2 tyrosine phosphorylation were investigated. Tyrosine 74-82 protein tyrosine kinase 2 beta Homo sapiens 68-73 22581436-7 2012 PACAP-38, but not vasoactive intestinal peptide (VIP), addition to NCI-H838 or H1299 cells significantly increased the tyrosine phosphorylation of PYK-2 after 2 min. Tyrosine 119-127 protein tyrosine kinase 2 beta Homo sapiens 147-152 22581436-8 2012 The increase in PYK-2 tyrosine phosphorylation caused by PACAP was inhibited by PACAP(6-38), U73122, or BAPTA, but not H89. Tyrosine 22-30 protein tyrosine kinase 2 beta Homo sapiens 16-21 22581436-9 2012 The results suggest that PAC1 regulates PYK-2 tyrosine phosphorylation in a calcium-dependent manner. Tyrosine 46-54 protein tyrosine kinase 2 beta Homo sapiens 40-45 23131831-6 2012 Mutation of the Y374 residue to phenylalanine (Y374F) specifically abolished SF-PTK-mediated tyrosine phosphorylation, leading to increased tumor necrosis factor alpha (TNF-alpha)-induced NF-kappaB activity. Tyrosine 93-101 protein tyrosine kinase 2 beta Homo sapiens 80-83 22923218-6 2012 Inhibition of Pyk2 activity using a pharmacological inhibitor, Tyrphostin A9, significantly attenuated CCR7-induced Pyk2 tyrosine phosphorylation, activation of cofilin and sequentially abolished F-actin rearrangment, diminished the chemotaxis and migration ability of SCCHN cells. Tyrosine 145-153 protein tyrosine kinase 2 beta Homo sapiens 14-18 22923218-6 2012 Inhibition of Pyk2 activity using a pharmacological inhibitor, Tyrphostin A9, significantly attenuated CCR7-induced Pyk2 tyrosine phosphorylation, activation of cofilin and sequentially abolished F-actin rearrangment, diminished the chemotaxis and migration ability of SCCHN cells. Tyrosine 145-153 protein tyrosine kinase 2 beta Homo sapiens 140-144 22699506-7 2012 We show that in a null mutant for Pyk2, a tyrosine-specific TKL, exposure to DIF-1 does not activate STATc. Tyrosine 42-50 protein tyrosine kinase 2 beta Homo sapiens 34-38 22382333-1 2012 Phosphorylation of tyrosine residues by protein tyrosine kinases (PTK) and phosphotyrosine/Src homology 2 (SH2) domain interactions are crucial not only for signal transduction but also for regulation of PTK activity. Tyrosine 19-27 protein tyrosine kinase 2 beta Homo sapiens 66-69 22581261-6 2012 After ligation of TLR9, DOCK8 became tyrosine-phosphorylated by Pyk2, bound the Src-family kinase Lyn and linked TLR9 to a Src-kinase Syk-transcription factor STAT3 cascade essential for TLR9-driven B cell proliferation and differentiation. Tyrosine 37-45 protein tyrosine kinase 2 beta Homo sapiens 64-68 22382333-1 2012 Phosphorylation of tyrosine residues by protein tyrosine kinases (PTK) and phosphotyrosine/Src homology 2 (SH2) domain interactions are crucial not only for signal transduction but also for regulation of PTK activity. Tyrosine 19-27 protein tyrosine kinase 2 beta Homo sapiens 204-207 22382333-3 2012 It has been reported that nitration of tyrosine residue caused peptides to be a poor substrate for PTK and that nitrotyrosine residues could bind to SH2 domains as a phosphotyrosine mimic to activate Src family kinase. Tyrosine 39-47 protein tyrosine kinase 2 beta Homo sapiens 99-102 20849950-5 2011 Utilizing differentially epitope-tagged Pyk2 constructs, we observed that Pyk2 forms oligomeric complexes in cells and that complex formation correlates positively with tyrosine phosphorylation. Tyrosine 169-177 protein tyrosine kinase 2 beta Homo sapiens 74-78 21820892-6 2011 Phosphorylation of the Tyr led to a loss of its electrochemical current, thus providing a sensing mechanism for PTK activity. Tyrosine 23-26 protein tyrosine kinase 2 beta Homo sapiens 112-115 21531776-5 2011 Prior EGFR-selective PTK inhibition with AG1478 or ErbB2-selective PTK inhibition with AG825 protected against TSP1-induced tyrosine phosphorylation of ZA proteins and barrier disruption. Tyrosine 124-132 protein tyrosine kinase 2 beta Homo sapiens 67-70 21238585-1 2011 The proline-rich tyrosine kinase 2, Pyk2, is a focal adhesion related kinase expressed in T cells that is tyrosine phosphorylated and activated by integrin, chemokine or T cell receptor stimulation. Tyrosine 17-25 protein tyrosine kinase 2 beta Homo sapiens 36-40 21357692-0 2011 Nephrocystin-4 regulates Pyk2-induced tyrosine phosphorylation of nephrocystin-1 to control targeting to monocilia. Tyrosine 38-46 protein tyrosine kinase 2 beta Homo sapiens 25-29 21357692-5 2011 NPHP4 counteracts Pyk2-mediated phosphorylation of three defined tyrosine residues of NPHP1 thereby controlling binding of NPHP1 to the trans-Golgi sorting protein PACS-1. Tyrosine 65-73 protein tyrosine kinase 2 beta Homo sapiens 18-22 21429240-11 2010 RESULTS: Expression of a mutant form of Pyk2 lacking the autophosphorylation site (Tyr-402) blocks pUS28-mediated SMC migration in response to CCL5, while the kinase-inactive Pyk2 mutant failed to elicit the same negative effect on migration. Tyrosine 83-86 protein tyrosine kinase 2 beta Homo sapiens 40-44 21429240-12 2010 pUS28 stimulation with CCL5 results in ligand-dependent and calcium-dependent phosphorylation of Pyk2 Tyr-402 and induced the formation of an active Pyk2 kinase complex containing several novel Pyk2 binding proteins. Tyrosine 102-105 protein tyrosine kinase 2 beta Homo sapiens 97-101 22137642-2 2010 Several factors are involved in this regulatory mechanism, such as serine/arginine-rich (SR) proteins, the Cdc2-like kinase (Clk) family, the dual-specificity tyrosine phosphorylation regulated kinases, the SR protein kinases (SRPK) 1 and 2, the protein kinase B (PKB,Akt), and the DNA topoisomerase I (DNA topo I). Tyrosine 159-167 protein tyrosine kinase 2 beta Homo sapiens 246-262 20688918-10 2010 These findings are consistent with the possibility that Pyk2 acts as an early sensor of numerous extracellular signals that trigger a Ca(2+) flux and/or reactive oxygen species to amplify tyrosine phosphorylation signaling events. Tyrosine 188-196 protein tyrosine kinase 2 beta Homo sapiens 56-60 20507587-9 2010 Pyk2 was tyrosine phosphorylated upon adhesion of dHL60 cells to plated fibrinogen in the presence of fMLP. Tyrosine 9-17 protein tyrosine kinase 2 beta Homo sapiens 0-4 20543098-6 2010 During IL-15-mediated NK cell priming, SOCS2 interacted with phosphorylated proline-rich tyrosine kinase 2 (Pyk2) at tyrosine 402 (p-Pyk2(Tyr402)) and induced the proteasome-mediated degradation of p-Pyk2(Tyr402) via ubiquitination. Tyrosine 89-97 protein tyrosine kinase 2 beta Homo sapiens 108-112 20543098-6 2010 During IL-15-mediated NK cell priming, SOCS2 interacted with phosphorylated proline-rich tyrosine kinase 2 (Pyk2) at tyrosine 402 (p-Pyk2(Tyr402)) and induced the proteasome-mediated degradation of p-Pyk2(Tyr402) via ubiquitination. Tyrosine 89-97 protein tyrosine kinase 2 beta Homo sapiens 133-137 20543098-6 2010 During IL-15-mediated NK cell priming, SOCS2 interacted with phosphorylated proline-rich tyrosine kinase 2 (Pyk2) at tyrosine 402 (p-Pyk2(Tyr402)) and induced the proteasome-mediated degradation of p-Pyk2(Tyr402) via ubiquitination. Tyrosine 89-97 protein tyrosine kinase 2 beta Homo sapiens 133-137 20303997-1 2010 The protein tyrosine kinase (PTK) inhibitor genistein has been widely used to examine potential effects of tyrosine phosphorylation on neurotransmitter function. Tyrosine 12-20 protein tyrosine kinase 2 beta Homo sapiens 29-32 20507587-10 2010 By contrast, tyrosine phosphorylation of Pyk2 was insignificant in dHL60 cells treated in suspension with fMLP. Tyrosine 13-21 protein tyrosine kinase 2 beta Homo sapiens 41-45 20381867-3 2010 In this study, the TCR-induced phosphorylation kinetics of Pyk2 tyrosines 402 and 580 were characterized in human T cells. Tyrosine 64-73 protein tyrosine kinase 2 beta Homo sapiens 59-63 19861182-11 2010 Pyk2 phosphorylated on Tyr-402 colocalized with paxillin in focal contacts of untreated cells, while it was barely detected upon exposure to particles. Tyrosine 23-26 protein tyrosine kinase 2 beta Homo sapiens 0-4 18954908-6 2009 Inhibition of Pyk2 activity using a pharmacological inhibitor, Tyrphostin A9 significantly attenuated LPS-induced Pyk2 tyrosine phosphorylation, p38 MAP kinase (MAPK) activation, NF-kappaB activation, and MCP-1 expression. Tyrosine 119-127 protein tyrosine kinase 2 beta Homo sapiens 14-18 20028775-0 2010 The T cell receptor-mediated phosphorylation of Pyk2 tyrosines 402 and 580 occurs via a distinct mechanism than other receptor systems. Tyrosine 53-62 protein tyrosine kinase 2 beta Homo sapiens 48-52 20028775-4 2010 To address this issue, the role of individual signaling pathways in the phosphorylation of Pyk2 tyrosines 402 and 580 upon TCR activation was assessed in human T cells. Tyrosine 96-105 protein tyrosine kinase 2 beta Homo sapiens 91-95 20028775-5 2010 In contrast to other receptor systems, the TCR-induced phosphorylation of Pyk2 tyrosines 402 and 580 was dependent on the Src family kinases, Fyn or Lck. Tyrosine 79-88 protein tyrosine kinase 2 beta Homo sapiens 74-78 20028775-6 2010 Interestingly, the TCR-mediated phosphorylation of Pyk2 tyrosines 402 and 580 did not require Ca(2+) influx, ZAP-70 activation, actin cytoskeleton rearrangement, or PI3K function. Tyrosine 56-65 protein tyrosine kinase 2 beta Homo sapiens 51-55 19857473-6 2010 By western blotting, proline-rich tyrosine kinase 2 (PYK2) was phosphorylated at Tyr-402 in response to CKLF1 and this phosphorylation was apparently suppressed by phospholipase C-gamma inhibitor U73122, but not extracellular Ca(2+) chelator EGTA. Tyrosine 81-84 protein tyrosine kinase 2 beta Homo sapiens 53-57 19561089-4 2009 In this report we show that BCR signaling and integrin signaling collaborate to induce the phosphorylation of Pyk2 and FAK on key tyrosine residues, a modification that increases the kinase activity of Pyk2 and FAK. Tyrosine 130-138 protein tyrosine kinase 2 beta Homo sapiens 110-114 19561089-4 2009 In this report we show that BCR signaling and integrin signaling collaborate to induce the phosphorylation of Pyk2 and FAK on key tyrosine residues, a modification that increases the kinase activity of Pyk2 and FAK. Tyrosine 130-138 protein tyrosine kinase 2 beta Homo sapiens 202-206 19380485-5 2009 Furthermore, catalytically active Src promotes dynamin-Pyk2 association, and mutating specific Src-phosphorylated tyrosine residues in dynamin blunts the dynamin-induced decrease in Pyk2 phosphorylation. Tyrosine 114-122 protein tyrosine kinase 2 beta Homo sapiens 55-59 19380485-5 2009 Furthermore, catalytically active Src promotes dynamin-Pyk2 association, and mutating specific Src-phosphorylated tyrosine residues in dynamin blunts the dynamin-induced decrease in Pyk2 phosphorylation. Tyrosine 114-122 protein tyrosine kinase 2 beta Homo sapiens 182-186 20074345-6 2010 Analysis of tyrosine kinase-mediated cell survival and growth signaling pathways revealed increased levels of tyrosine-phosphorylation of PECAM-1 and Pyk2 in cell aggregates. Tyrosine 12-20 protein tyrosine kinase 2 beta Homo sapiens 150-154 19207108-10 2009 These results indicate that Pyk2 is a critical signalling molecule downstream of beta3 integrin tyrosine phosphorylation and mediates Vav1 recruitment to accomplish actin reorganization necessary for adhesion. Tyrosine 96-104 protein tyrosine kinase 2 beta Homo sapiens 28-32 17581824-8 2007 Consistent with the requirement of tyrosine 402, dexamethasone induces rapid Pyk2 phosphorylation in this residue. Tyrosine 35-43 protein tyrosine kinase 2 beta Homo sapiens 77-81 18687633-8 2008 These data suggest that a higher proportion of the EGFR mutant carcinoma cells may exhibit activation of the phosphatidylinositol 3-kinase/protein kinase B (AKT)/mammalian target of rapamycin (MTOR) pathway through Tyr-1148 and Tyr-1068 and suppression of IRS-1 Ser-612, altered heterodimerization with ERBB2, reduced response to transforming growth factor beta suppression, and reduced ubiquitination/degradation of the EGFR through EGFR Tyr-1045, thus providing a survival advantage. Tyrosine 215-218 protein tyrosine kinase 2 beta Homo sapiens 139-155 18687633-8 2008 These data suggest that a higher proportion of the EGFR mutant carcinoma cells may exhibit activation of the phosphatidylinositol 3-kinase/protein kinase B (AKT)/mammalian target of rapamycin (MTOR) pathway through Tyr-1148 and Tyr-1068 and suppression of IRS-1 Ser-612, altered heterodimerization with ERBB2, reduced response to transforming growth factor beta suppression, and reduced ubiquitination/degradation of the EGFR through EGFR Tyr-1045, thus providing a survival advantage. Tyrosine 228-231 protein tyrosine kinase 2 beta Homo sapiens 139-155 18687633-8 2008 These data suggest that a higher proportion of the EGFR mutant carcinoma cells may exhibit activation of the phosphatidylinositol 3-kinase/protein kinase B (AKT)/mammalian target of rapamycin (MTOR) pathway through Tyr-1148 and Tyr-1068 and suppression of IRS-1 Ser-612, altered heterodimerization with ERBB2, reduced response to transforming growth factor beta suppression, and reduced ubiquitination/degradation of the EGFR through EGFR Tyr-1045, thus providing a survival advantage. Tyrosine 228-231 protein tyrosine kinase 2 beta Homo sapiens 139-155 18483407-2 2008 We found that fluid shear stress induces the association of eNOS with the proline-rich tyrosine kinase 2 (PYK2) in endothelial cells and that the eNOS immunoprecipitated from eNOS- and PYK2-overexpressing HEK293 cells was tyrosine-phosphorylated on Tyr657. Tyrosine 87-95 protein tyrosine kinase 2 beta Homo sapiens 106-110 18483407-2 2008 We found that fluid shear stress induces the association of eNOS with the proline-rich tyrosine kinase 2 (PYK2) in endothelial cells and that the eNOS immunoprecipitated from eNOS- and PYK2-overexpressing HEK293 cells was tyrosine-phosphorylated on Tyr657. Tyrosine 87-95 protein tyrosine kinase 2 beta Homo sapiens 185-189 18031286-6 2008 CAKbeta/PYK2 is known to be prominently tyrosine phosphorylated when overexpressed from cDNA. Tyrosine 40-48 protein tyrosine kinase 2 beta Homo sapiens 0-7 18031286-6 2008 CAKbeta/PYK2 is known to be prominently tyrosine phosphorylated when overexpressed from cDNA. Tyrosine 40-48 protein tyrosine kinase 2 beta Homo sapiens 8-12 18031286-7 2008 The enhanced tyrosine phosphorylation was inhibited by W7, an inhibitor of calmodulin, and by a cell-permeable Ca2+ chelator and was almost defective in the LQ/AA-mutant CAKbeta/PYK2. Tyrosine 13-21 protein tyrosine kinase 2 beta Homo sapiens 170-177 18031286-7 2008 The enhanced tyrosine phosphorylation was inhibited by W7, an inhibitor of calmodulin, and by a cell-permeable Ca2+ chelator and was almost defective in the LQ/AA-mutant CAKbeta/PYK2. Tyrosine 13-21 protein tyrosine kinase 2 beta Homo sapiens 178-182 18198130-2 2008 Pyk2 expression has been known to be restricted to neuronal and hematopoietic cells and Pyk2 tyrosine phosphorylation and its kinase activity is important for the function of monocytes/macrophages. Tyrosine 93-101 protein tyrosine kinase 2 beta Homo sapiens 88-92 17643958-2 2007 In this study, we demonstrated that mAChR stimulation also induced a time-dependent increase in the tyrosine phosphorylation of proline-rich tyrosine kinase 2 (Pyk2), which was prevented by pretreatment of thyroid epithelial cells with the specific Src-family tyrosine kinase inhibitor PP2. Tyrosine 100-108 protein tyrosine kinase 2 beta Homo sapiens 128-158 17643958-2 2007 In this study, we demonstrated that mAChR stimulation also induced a time-dependent increase in the tyrosine phosphorylation of proline-rich tyrosine kinase 2 (Pyk2), which was prevented by pretreatment of thyroid epithelial cells with the specific Src-family tyrosine kinase inhibitor PP2. Tyrosine 100-108 protein tyrosine kinase 2 beta Homo sapiens 160-164 17875741-10 2007 EphA4 activation decreases tyrosine phosphorylation of the scaffolding protein Crk-associated substrate (Cas) and the tyrosine kinases focal adhesion kinase (FAK) and proline-rich tyrosine kinase 2 (Pyk2) and also reduces the association of Cas with the Src family kinase Fyn and the adaptor Crk. Tyrosine 27-35 protein tyrosine kinase 2 beta Homo sapiens 199-203 17785844-0 2007 ICAM-1-mediated, Src- and Pyk2-dependent vascular endothelial cadherin tyrosine phosphorylation is required for leukocyte transendothelial migration. Tyrosine 71-79 protein tyrosine kinase 2 beta Homo sapiens 26-30 18655171-12 2008 Inhibiting protein tyrosine kinase Pyk2, an upstream regulator of GSK-3beta, caused inhibition on IFN-gamma/LPS-induced GSK-3beta phosphorylation at tyrosine 216 and iNOS/NO biosynthesis. Tyrosine 19-27 protein tyrosine kinase 2 beta Homo sapiens 35-39 18367725-4 2008 TAT-Pyk2-CT blocked tyrosine phosphorylation of Pyk2 caused by beta(2)-integrin adhesion, but did not block adhesion of eosinophils to plated BSA. Tyrosine 20-28 protein tyrosine kinase 2 beta Homo sapiens 0-11 18367725-4 2008 TAT-Pyk2-CT blocked tyrosine phosphorylation of Pyk2 caused by beta(2)-integrin adhesion, but did not block adhesion of eosinophils to plated BSA. Tyrosine 20-28 protein tyrosine kinase 2 beta Homo sapiens 4-8 16452634-6 2006 Inactivation or knockdown of Pyk2 inhibits LPA-induced (but not basal) tyrosine phosphorylation of GSK-3 and partially inhibits LPA-induced neurite retraction, similar to what is observed following GSK-3 inhibition. Tyrosine 71-79 protein tyrosine kinase 2 beta Homo sapiens 29-33 17317777-7 2007 Consistently, fat tissue fragments stimulated with insulin demonstrated that tyrosine phosphorylation and signal transduction to Akt/protein kinase B in omental fat was not inferior to that observable in sc fat. Tyrosine 77-85 protein tyrosine kinase 2 beta Homo sapiens 133-149 17279354-8 2007 Exposure of L6 myotubes to hrCRP reduced insulin-stimulated phosphorylation of IRS-1 at Tyr(632), a site essential for engaging p85 subunit of phosphatidylinositol-3 kinase (PI-3K), protein kinase B (Akt) activation and glycogen synthase kinase-3 (GSK-3) phosphorylation. Tyrosine 88-91 protein tyrosine kinase 2 beta Homo sapiens 182-198 17055484-4 2007 Challenge of HBMECs with HGF activated the c-jun amino-terminal kinase (JNK), increased phosphorylation of the proline-rich tyrosine kinase 2 (Pyk-2) at Tyr(402) and activated c-Src. Tyrosine 153-156 protein tyrosine kinase 2 beta Homo sapiens 111-141 17055484-4 2007 Challenge of HBMECs with HGF activated the c-jun amino-terminal kinase (JNK), increased phosphorylation of the proline-rich tyrosine kinase 2 (Pyk-2) at Tyr(402) and activated c-Src. Tyrosine 153-156 protein tyrosine kinase 2 beta Homo sapiens 143-148 17055484-7 2007 Moreover, expression of a mutant Pyk-2 construct prevented HGF-induced Pyk-2 phosphorylation at Tyr(402) and stimulation of HBMEC migration. Tyrosine 96-99 protein tyrosine kinase 2 beta Homo sapiens 33-38 17055484-7 2007 Moreover, expression of a mutant Pyk-2 construct prevented HGF-induced Pyk-2 phosphorylation at Tyr(402) and stimulation of HBMEC migration. Tyrosine 96-99 protein tyrosine kinase 2 beta Homo sapiens 71-76 16574377-0 2006 Differential regulation of Pyk2 phosphorylation at Tyr-402 and Tyr-580 in intestinal epithelial cells: roles of calcium, Src, Rho kinase, and the cytoskeleton. Tyrosine 51-54 protein tyrosine kinase 2 beta Homo sapiens 27-31 16574377-0 2006 Differential regulation of Pyk2 phosphorylation at Tyr-402 and Tyr-580 in intestinal epithelial cells: roles of calcium, Src, Rho kinase, and the cytoskeleton. Tyrosine 63-66 protein tyrosine kinase 2 beta Homo sapiens 27-31 16821235-7 2006 We also show that Pyk2, a tyrosine kinase involved in cytoskeleton rearrangements and B cell migration, is a downstream target of both CXCL13 and S1P signaling and that Rap activation is important for CXCL13 and S1P to stimulate tyrosine phosphorylation of Pyk2, a modification that increases Pyk2 kinase activity. Tyrosine 26-34 protein tyrosine kinase 2 beta Homo sapiens 18-22 16998541-10 2006 Moreover, H2O2-induced PKB phosphorylation was associated with increased tyrosine phosphorylation of c-Src and Pyk2 in an AG1024- and PP2-inhibitable manner. Tyrosine 73-81 protein tyrosine kinase 2 beta Homo sapiens 23-26 16546139-1 2006 We showed that cyclic strain (CS) of osteoblastic cells induced tyrosine phosphorylation of two homologous tyrosine kinases FAK and PYK2, and of two homologous adaptor proteins paxillin and Hic5, with similar kinetics. Tyrosine 64-72 protein tyrosine kinase 2 beta Homo sapiens 132-136 16639034-5 2006 Therefore, tyrosine phosphorylation-dependent signaling involving receptor tyrosine kinases, mitogen-activated protein kinases, Abl, Src, and Pyk2 is known to be initiated or amplified by reactive oxidants. Tyrosine 11-19 protein tyrosine kinase 2 beta Homo sapiens 142-146 17327418-7 2007 Moreover, Lad was required for the chemokine-dependent tyrosine phosphorylation of focal adhesion molecules that included Pyk2 and paxillin. Tyrosine 55-63 protein tyrosine kinase 2 beta Homo sapiens 122-126 17321230-8 2007 CT induced dephosphorylation at Tyr(402) of Pyk2 and decreased its labeling at peripheral adhesion region, which would prevent formation of the Pyk2/Src complex in this region. Tyrosine 32-35 protein tyrosine kinase 2 beta Homo sapiens 44-48 16574377-1 2006 The calcium-dependent proline-rich tyrosine kinase Pyk2 is activated by tyrosine phosphorylation, associates with focal adhesion proteins, and has been linked to proliferative and migratory responses in a variety of mesenchymal and epithelial cell types. Tyrosine 35-43 protein tyrosine kinase 2 beta Homo sapiens 51-55 16574377-2 2006 Full Pyk2 activation requires phosphorylation at functionally distinct sites, including autophosphorylation site Tyr-402 and catalytic domain site Tyr-580, though the mechanisms involved are unclear. Tyrosine 113-116 protein tyrosine kinase 2 beta Homo sapiens 5-9 16574377-2 2006 Full Pyk2 activation requires phosphorylation at functionally distinct sites, including autophosphorylation site Tyr-402 and catalytic domain site Tyr-580, though the mechanisms involved are unclear. Tyrosine 147-150 protein tyrosine kinase 2 beta Homo sapiens 5-9 16574377-3 2006 The pathways mediating Pyk2 phosphorylation at Tyr-402 and Tyr-580 were therefore investigated. Tyrosine 47-50 protein tyrosine kinase 2 beta Homo sapiens 23-27 16821235-0 2006 The Rap GTPases mediate CXCL13- and sphingosine1-phosphate-induced chemotaxis, adhesion, and Pyk2 tyrosine phosphorylation in B lymphocytes. Tyrosine 98-106 protein tyrosine kinase 2 beta Homo sapiens 93-97 15994200-0 2005 Regulation of protein kinase B tyrosine phosphorylation by thyroid-specific oncogenic RET/PTC kinases. Tyrosine 31-39 protein tyrosine kinase 2 beta Homo sapiens 14-30 15994200-4 2005 RET/PTC-transfected cells showed tyrosine phosphorylation of endogenous and exogenous PKB, which was independent of phosphorylation of T308 and S473 regulated by the upstream kinases phosphoinositide-dependent kinase-1 and -2, respectively. Tyrosine 33-41 protein tyrosine kinase 2 beta Homo sapiens 86-89 15994200-6 2005 RET/PTC-mediated tyrosine phosphorylation results in the activation of PKB kinase activity. Tyrosine 17-25 protein tyrosine kinase 2 beta Homo sapiens 71-74 16055703-7 2005 This results in the tyrosine phosphorylation of Plexin-B1, the rapid recruitment of a multimeric signaling complex that includes PYK2, Src, and PI3K to Plexin-B1 and the activation of Akt. Tyrosine 20-28 protein tyrosine kinase 2 beta Homo sapiens 129-133 15944312-7 2005 Both dominant negative pyk2 and a pyk2-selective inhibitor prevented restoration of ROS production induced by TNF-alpha, GM-CSF, and platelet-activating factor, and this loss of pyk2 activity resulted in decreased Vav1 tyrosine phosphorylation and subsequent Rac2 activation. Tyrosine 219-227 protein tyrosine kinase 2 beta Homo sapiens 23-27 15944312-7 2005 Both dominant negative pyk2 and a pyk2-selective inhibitor prevented restoration of ROS production induced by TNF-alpha, GM-CSF, and platelet-activating factor, and this loss of pyk2 activity resulted in decreased Vav1 tyrosine phosphorylation and subsequent Rac2 activation. Tyrosine 219-227 protein tyrosine kinase 2 beta Homo sapiens 34-38 15944312-7 2005 Both dominant negative pyk2 and a pyk2-selective inhibitor prevented restoration of ROS production induced by TNF-alpha, GM-CSF, and platelet-activating factor, and this loss of pyk2 activity resulted in decreased Vav1 tyrosine phosphorylation and subsequent Rac2 activation. Tyrosine 219-227 protein tyrosine kinase 2 beta Homo sapiens 34-38 15585656-0 2005 Ephrin-A1 binding to CD4+ T lymphocytes stimulates migration and induces tyrosine phosphorylation of PYK2. Tyrosine 73-81 protein tyrosine kinase 2 beta Homo sapiens 101-105 15601262-0 2005 Interferon-gamma-dependent tyrosine phosphorylation of MEKK4 via Pyk2 is regulated by annexin II and SHP2 in keratinocytes. Tyrosine 27-35 protein tyrosine kinase 2 beta Homo sapiens 65-69 15695828-0 2005 Tyrosine phosphorylation of beta3 integrin provides a binding site for Pyk2. Tyrosine 0-8 protein tyrosine kinase 2 beta Homo sapiens 71-75 15695828-6 2005 Here we show that alpha(V)beta(3) ligation induces Pyk2-Tyr-402 phosphorylation and its association with the beta(3) cytoplasmic tail in a beta(3)-Tyr-747 phosphorylation-dependent manner. Tyrosine 56-59 protein tyrosine kinase 2 beta Homo sapiens 51-55 15695828-7 2005 Pyk2 binding to the beta(3) cytoplasmic tail is direct and dependent upon Pyk2-Tyr-402 and beta(3) -Tyr-747 phosphorylations. Tyrosine 79-82 protein tyrosine kinase 2 beta Homo sapiens 0-4 15695828-7 2005 Pyk2 binding to the beta(3) cytoplasmic tail is direct and dependent upon Pyk2-Tyr-402 and beta(3) -Tyr-747 phosphorylations. Tyrosine 79-82 protein tyrosine kinase 2 beta Homo sapiens 74-78 15778498-0 2005 Proline-rich tyrosine kinase 2 (Pyk2) mediates vascular endothelial-cadherin-based cell-cell adhesion by regulating beta-catenin tyrosine phosphorylation. Tyrosine 13-21 protein tyrosine kinase 2 beta Homo sapiens 32-36 15778498-7 2005 Conversely, the inhibition of Pyk2 activity in endothelial cells by the expression of CRNK (CADTK/CAKbeta-related non-kinase), an N-terminal deletion mutant that acts in a dominant negative fashion, not only abolishes the increase in beta-catenin tyrosine phosphorylation but also prevents the loss of endothelial cell-cell contact. Tyrosine 247-255 protein tyrosine kinase 2 beta Homo sapiens 30-34 15778498-7 2005 Conversely, the inhibition of Pyk2 activity in endothelial cells by the expression of CRNK (CADTK/CAKbeta-related non-kinase), an N-terminal deletion mutant that acts in a dominant negative fashion, not only abolishes the increase in beta-catenin tyrosine phosphorylation but also prevents the loss of endothelial cell-cell contact. Tyrosine 247-255 protein tyrosine kinase 2 beta Homo sapiens 92-97 15778498-8 2005 These results implicate Pyk2 in the reduced cell-cell adhesion induced by the Rac-mediated production of ROS through the tyrosine phosphorylation of beta-catenin. Tyrosine 121-129 protein tyrosine kinase 2 beta Homo sapiens 24-28 15585656-7 2005 In particular, proline-rich tyrosine kinase 2 (PYK2) is phosphorylated on tyrosine residues 402 and 580. Tyrosine 28-36 protein tyrosine kinase 2 beta Homo sapiens 47-51 16413287-2 2005 Following binding, Pyk2 directly phosphorylates ASAP1 on tyrosine residues 308 and 782 in vitro and in cells. Tyrosine 57-65 protein tyrosine kinase 2 beta Homo sapiens 19-23 15634339-2 2005 Directly after surgery and cell isolation, adipocytes were insulin resistant, but this was reversed after overnight incubation in 10% CO(2) at 37 degrees C. Tyrosine phosphorylation of the insulin receptor and insulin receptor substrate (IRS)1 was insulin sensitive, but protein kinase B (PKB) and downstream metabolic effects exhibited insulin resistance that was reversed by overnight incubation. Tyrosine 157-165 protein tyrosine kinase 2 beta Homo sapiens 271-287 15634339-2 2005 Directly after surgery and cell isolation, adipocytes were insulin resistant, but this was reversed after overnight incubation in 10% CO(2) at 37 degrees C. Tyrosine phosphorylation of the insulin receptor and insulin receptor substrate (IRS)1 was insulin sensitive, but protein kinase B (PKB) and downstream metabolic effects exhibited insulin resistance that was reversed by overnight incubation. Tyrosine 157-165 protein tyrosine kinase 2 beta Homo sapiens 289-292 15499613-0 2005 Tyrosine phosphorylation of PYK2 mediates heregulin-induced glioma invasion: novel heregulin/HER3-stimulated signaling pathway in glioma. Tyrosine 0-8 protein tyrosine kinase 2 beta Homo sapiens 28-32 15499613-5 2005 Our data show that in a glioma-derived cell line the cytoplasmic tyrosine kinase PYK2 is constitutively associated with HER3 and that stimulation with Heregulin results in PYK2 tyrosine phosphorylation. Tyrosine 65-73 protein tyrosine kinase 2 beta Homo sapiens 81-85 15499613-5 2005 Our data show that in a glioma-derived cell line the cytoplasmic tyrosine kinase PYK2 is constitutively associated with HER3 and that stimulation with Heregulin results in PYK2 tyrosine phosphorylation. Tyrosine 65-73 protein tyrosine kinase 2 beta Homo sapiens 172-176 15345598-7 2005 SDF-1alpha/CXCL12 induced the tyrosine phosphorylation of Pyk2 and the association of Pyk2 with zeta chain-associated protein-70 kilodaltons (Zap-70) and Vav. Tyrosine 30-38 protein tyrosine kinase 2 beta Homo sapiens 58-62 16413287-4 2005 This material can be used for lipid-protein overlay assays and fluorimetric Arf-GTPase tests to show that the Pyk2-mediated tyrosine phosphorylation of ASAP1 modulates its GAP activity towards Arf1 in vitro. Tyrosine 124-132 protein tyrosine kinase 2 beta Homo sapiens 110-114 15158121-4 2004 ET-1, PE or the Ca(2+) inophore, ionomycin, stimulated a rapid increase in tyrosine phosphorylation of Pyk2. Tyrosine 75-83 protein tyrosine kinase 2 beta Homo sapiens 103-107 15493507-4 2004 We observe tyrosine phosphorylation of Pyk2, p38, and ERK1/2 but no measurable increase in free AA, 12-hydroxyeicosatetraenoic acid, or thromboxane A2. Tyrosine 11-19 protein tyrosine kinase 2 beta Homo sapiens 39-43 15105428-7 2004 Overexpression of Pyk2 alone led to its spontaneous activation and tyrosine phosphorylation, resulting in activation of Stat5B, indicated by the reporter GFP-Stat5B. Tyrosine 67-75 protein tyrosine kinase 2 beta Homo sapiens 18-22 15105428-8 2004 These effects were completely dependent upon Tyr(402), the autophosphorylation site of Pyk2, which allows recruitment of Src family members for further activating phosphorylations at other sites on Pyk2. Tyrosine 45-48 protein tyrosine kinase 2 beta Homo sapiens 87-91 15105428-8 2004 These effects were completely dependent upon Tyr(402), the autophosphorylation site of Pyk2, which allows recruitment of Src family members for further activating phosphorylations at other sites on Pyk2. Tyrosine 45-48 protein tyrosine kinase 2 beta Homo sapiens 198-202 15105428-9 2004 In the presence of activated FGFR3, the activation of Pyk2 itself became independent of Tyr(402), indicating that FGFR3 activation circumvents the requirement for c-Src recruitment at Tyr(402) of Pyk2. Tyrosine 184-187 protein tyrosine kinase 2 beta Homo sapiens 54-58 15573143-4 2004 As a result, they promote an increase in protein tyrosine phosphorylation of several key components of the insulin signaling pathway, leading to the upregulation of phosphatidylinositol 3-kinase and protein kinase B, two enzymes involved in mediating GLUT-4 trans location and glucose transport. Tyrosine 49-57 protein tyrosine kinase 2 beta Homo sapiens 199-215 15213840-8 2004 Using specific antibodies, increased phosphorylation of PYK2 tyrosine residues 402 and 580 was observed. Tyrosine 61-69 protein tyrosine kinase 2 beta Homo sapiens 56-60 15158121-5 2004 The tyrosine phosphorylation of Pyk2 was suppressed by the Ca(2+) chelator, BAPTA. Tyrosine 4-12 protein tyrosine kinase 2 beta Homo sapiens 32-36 15126359-7 2004 Moreover, FAK expression was correlated with the phosphorylation of PYK2 on Tyr-881, a critical site for the PYK2 function in cell migration. Tyrosine 76-79 protein tyrosine kinase 2 beta Homo sapiens 68-72 15126359-7 2004 Moreover, FAK expression was correlated with the phosphorylation of PYK2 on Tyr-881, a critical site for the PYK2 function in cell migration. Tyrosine 76-79 protein tyrosine kinase 2 beta Homo sapiens 109-113 15056377-3 2004 Induction of Pyk2 tyrosine phosphorylation by CXCL8 is regulated by Src PTK activation, whereas it is unaffected by phosphatidylinositol 3-kinase activation. Tyrosine 18-26 protein tyrosine kinase 2 beta Homo sapiens 13-17 14963038-7 2004 Pyk2 expression in HeLa cells induces Stat3 reporter gene activation and Stat3 phosphorylation on amino acid residues Tyr-705 and Ser-727. Tyrosine 118-121 protein tyrosine kinase 2 beta Homo sapiens 0-4 14585963-0 2003 Pyk2- and Src-dependent tyrosine phosphorylation of PDK1 regulates focal adhesions. Tyrosine 24-32 protein tyrosine kinase 2 beta Homo sapiens 0-4 14645233-8 2004 In addition, characterization of signaling events revealed that Slit inhibits CXCL12-induced tyrosine phosphorylation of focal adhesion components such as RAFTK/Pyk2 at residues 580 and 881, focal adhesion kinase at residue 576, and paxillin. Tyrosine 93-101 protein tyrosine kinase 2 beta Homo sapiens 155-160 14645233-8 2004 In addition, characterization of signaling events revealed that Slit inhibits CXCL12-induced tyrosine phosphorylation of focal adhesion components such as RAFTK/Pyk2 at residues 580 and 881, focal adhesion kinase at residue 576, and paxillin. Tyrosine 93-101 protein tyrosine kinase 2 beta Homo sapiens 161-165 14654952-3 2004 Stimulation of T47D cells with heregulin (HRG) induced the tyrosine phosphorylation of RAFTK and the formation of a multiprotein complex. Tyrosine 59-67 protein tyrosine kinase 2 beta Homo sapiens 87-92 14654952-7 2004 Site-directed mutagenesis of Y881 aa within the RAFTK sequence abolished the binding of RAFTK to c-Abl, indicating that the tyrosine residue 881 of RAFTK is the c-Abl binding site within the RAFTK molecule. Tyrosine 124-132 protein tyrosine kinase 2 beta Homo sapiens 48-53 14654952-7 2004 Site-directed mutagenesis of Y881 aa within the RAFTK sequence abolished the binding of RAFTK to c-Abl, indicating that the tyrosine residue 881 of RAFTK is the c-Abl binding site within the RAFTK molecule. Tyrosine 124-132 protein tyrosine kinase 2 beta Homo sapiens 88-93 14654952-7 2004 Site-directed mutagenesis of Y881 aa within the RAFTK sequence abolished the binding of RAFTK to c-Abl, indicating that the tyrosine residue 881 of RAFTK is the c-Abl binding site within the RAFTK molecule. Tyrosine 124-132 protein tyrosine kinase 2 beta Homo sapiens 88-93 14654952-7 2004 Site-directed mutagenesis of Y881 aa within the RAFTK sequence abolished the binding of RAFTK to c-Abl, indicating that the tyrosine residue 881 of RAFTK is the c-Abl binding site within the RAFTK molecule. Tyrosine 124-132 protein tyrosine kinase 2 beta Homo sapiens 88-93 14657361-2 2003 For the fidelity of signal transduction, each PTK phosphorylates only one or a few proteins on specific Tyr residues. Tyrosine 104-107 protein tyrosine kinase 2 beta Homo sapiens 46-49 14657361-5 2003 C-terminal Src kinase (Csk) is a PTK that specifically phosphorylates Src family kinases on a C-terminal Tyr. Tyrosine 105-108 protein tyrosine kinase 2 beta Homo sapiens 33-36 14585963-5 2003 This critical function of Pyk2 is further supported by the observation that Pyk2 and tyrosine-phosphorylated PDK1 colocalize in focal adhesions after angiotensin II stimulation. Tyrosine 85-93 protein tyrosine kinase 2 beta Homo sapiens 26-30 14996908-7 2004 Time-dependent increases in colocalization of beta(3) with tyrosine 402 phosphorylated Pyk2 in similar adhesive structures was observed, providing evidence for downstream signaling complex formation. Tyrosine 59-67 protein tyrosine kinase 2 beta Homo sapiens 87-91 14730623-6 2004 MSU induced rapid tyrosine phosphorylation of Pyk-2 and FAK, their adaptor protein paxillin, and interacting kinase c-Src. Tyrosine 18-26 protein tyrosine kinase 2 beta Homo sapiens 46-51 12730223-2 2003 In the present study, treatment of chondrocytes with the 110-kDa FN-f or an activating antibody to the alpha5beta1 integrin was found to increase tyrosine autophosphorylation (Tyr-402) of the proline-rich tyrosine kinase-2 (PYK2) without significant change in autophosphorylation (Tyr-397) of focal adhesion kinase (FAK). Tyrosine 176-179 protein tyrosine kinase 2 beta Homo sapiens 192-222 12893833-3 2003 The present study reports the cloning and characterization of a novel adaptor protein, Pyk2/RAFTK-associated protein (PRAP), that specifically binds to Pyk2/RAFTK and inhibits alpha-synuclein tyrosine phosphorylation. Tyrosine 192-200 protein tyrosine kinase 2 beta Homo sapiens 87-91 12893833-3 2003 The present study reports the cloning and characterization of a novel adaptor protein, Pyk2/RAFTK-associated protein (PRAP), that specifically binds to Pyk2/RAFTK and inhibits alpha-synuclein tyrosine phosphorylation. Tyrosine 192-200 protein tyrosine kinase 2 beta Homo sapiens 92-97 12893833-7 2003 Hyperosmotic stress induced phosphorylation of tyrosine 125 of alpha-synuclein via Pyk2/RAFTK, which acted through Src family kinases. Tyrosine 47-55 protein tyrosine kinase 2 beta Homo sapiens 83-87 12893833-7 2003 Hyperosmotic stress induced phosphorylation of tyrosine 125 of alpha-synuclein via Pyk2/RAFTK, which acted through Src family kinases. Tyrosine 47-55 protein tyrosine kinase 2 beta Homo sapiens 88-93 12844492-7 2003 Additionally, FAK and related adhesion focal tyrosine kinase (RAFTK)/Pyk2 kinases were tyrosine-phosphorylated by VEGF and found to be important for focal adhesion sites. Tyrosine 45-53 protein tyrosine kinase 2 beta Homo sapiens 62-67 12844492-7 2003 Additionally, FAK and related adhesion focal tyrosine kinase (RAFTK)/Pyk2 kinases were tyrosine-phosphorylated by VEGF and found to be important for focal adhesion sites. Tyrosine 45-53 protein tyrosine kinase 2 beta Homo sapiens 69-73 12900387-0 2003 Effects of nonselective cation channels and PI3K on endothelin-1-induced PYK2 tyrosine phosphorylation in C6 glioma cells. Tyrosine 78-86 protein tyrosine kinase 2 beta Homo sapiens 73-77 12771146-5 2003 Pyk2 directly phosphorylates ASAP1 on tyrosine residues in vitro and increases ASAP1 tyrosine phosphorylation when co-expressed in HEK293T cells. Tyrosine 38-46 protein tyrosine kinase 2 beta Homo sapiens 0-4 12771146-5 2003 Pyk2 directly phosphorylates ASAP1 on tyrosine residues in vitro and increases ASAP1 tyrosine phosphorylation when co-expressed in HEK293T cells. Tyrosine 85-93 protein tyrosine kinase 2 beta Homo sapiens 0-4 12771146-6 2003 Phosphorylation of tyrosine 308 and 782 affects the phosphoinositide binding profile of ASAP1, and fluorimetric Arf-GTPase assays with purified proteins revealed an inhibition of ASAP1 GTPase-activating protein activity by Pyk2-mediated tyrosine phosphorylation. Tyrosine 19-27 protein tyrosine kinase 2 beta Homo sapiens 223-227 12771146-7 2003 We therefore provide evidence for a functional interaction between Pyk2 and ASAP1 and a regulation of ASAP1 and hence Arf1 activity by Pyk2-mediated tyrosine phosphorylation. Tyrosine 149-157 protein tyrosine kinase 2 beta Homo sapiens 67-71 12771146-7 2003 We therefore provide evidence for a functional interaction between Pyk2 and ASAP1 and a regulation of ASAP1 and hence Arf1 activity by Pyk2-mediated tyrosine phosphorylation. Tyrosine 149-157 protein tyrosine kinase 2 beta Homo sapiens 135-139 12730223-2 2003 In the present study, treatment of chondrocytes with the 110-kDa FN-f or an activating antibody to the alpha5beta1 integrin was found to increase tyrosine autophosphorylation (Tyr-402) of the proline-rich tyrosine kinase-2 (PYK2) without significant change in autophosphorylation (Tyr-397) of focal adhesion kinase (FAK). Tyrosine 176-179 protein tyrosine kinase 2 beta Homo sapiens 224-228 12682013-8 2003 Therefore, developmental regulation of Src-PTK-dependent tyrosine phosphorylation of QKI suggests a novel mechanism for accelerating CNS myelinogenesis via regulating mRNA metabolism. Tyrosine 57-65 protein tyrosine kinase 2 beta Homo sapiens 43-46 12895435-3 2003 Insulin applied to native porcine aortic endothelial cells elicited the tyrosine phosphorylation of the insulin receptor and receptor substrate, the subsequent activation of phosphatidylinositol 3-kinase (PI 3-K), Akt (protein kinase B), and ERK1/2. Tyrosine 72-80 protein tyrosine kinase 2 beta Homo sapiens 219-235 12674328-6 2003 Treatment of osteoclasts with TNF-alpha and soluble osteopontin were found to stimulate tyrosine phosphorylation of both leupaxin and leupaxin-associated PYK2. Tyrosine 88-96 protein tyrosine kinase 2 beta Homo sapiens 154-158 12514172-4 2003 By infecting pre-fusion osteoclasts using recombinant adenovirus expressing PYK2 and its mutants, we demonstrated that mutation at the autophosphorylation site (Y402F) abolishes PYK2 association with c-Src and reduces significantly phosphorylation at tyrosines 579/580 and 881 resulting in inhibition of osteoclast spreading and bone resorption. Tyrosine 251-260 protein tyrosine kinase 2 beta Homo sapiens 76-80 12514172-8 2003 Whereas PYK2(Y402F) dominant negatively blocks osteoclast spreading and bone resorption, PYK2(K457A) may function in part as an adaptor by initially recruiting c-Src to the adhesion complex, which appears to activate PYK2 by phosphorylating additional tyrosines in its regulatory and C-terminal domains. Tyrosine 252-261 protein tyrosine kinase 2 beta Homo sapiens 89-93 12514172-8 2003 Whereas PYK2(Y402F) dominant negatively blocks osteoclast spreading and bone resorption, PYK2(K457A) may function in part as an adaptor by initially recruiting c-Src to the adhesion complex, which appears to activate PYK2 by phosphorylating additional tyrosines in its regulatory and C-terminal domains. Tyrosine 252-261 protein tyrosine kinase 2 beta Homo sapiens 89-93 12514172-9 2003 We thus concluded that phosphorylation at Tyr-402 in PYK2 is essential in the regulation of adhesion-dependent cytoskeletal organization in osteoclasts. Tyrosine 42-45 protein tyrosine kinase 2 beta Homo sapiens 53-57 12626562-5 2003 Moreover, we found that Pyk-2 is associated with the Rac guanine nucleotide exchange factor Vav, which undergoes tyrosine phosphorylation upon integrin triggering. Tyrosine 113-121 protein tyrosine kinase 2 beta Homo sapiens 24-29 12408997-2 2002 Based upon the fact that calmodulin shows its action on other enzymes through PTK caused phosphorylation of tyrosine residues of calmodulin itself, we studied whether a calmodulin-dependent pathway is involved in the action of hypotonicity on ENaC. Tyrosine 108-116 protein tyrosine kinase 2 beta Homo sapiens 78-81 12519755-11 2003 Comparisons of signaling pathways induced by CXCL12 in JE6.1 and J45.01 cells revealed that CD45 might moderately regulate the tyrosine phosphorylation of the focal adhesion components the related adhesion focal tyrosine kinase/Pyk2, focal adhesion kinase, p130Cas, and paxillin. Tyrosine 127-135 protein tyrosine kinase 2 beta Homo sapiens 189-227 12519755-11 2003 Comparisons of signaling pathways induced by CXCL12 in JE6.1 and J45.01 cells revealed that CD45 might moderately regulate the tyrosine phosphorylation of the focal adhesion components the related adhesion focal tyrosine kinase/Pyk2, focal adhesion kinase, p130Cas, and paxillin. Tyrosine 127-135 protein tyrosine kinase 2 beta Homo sapiens 228-232 12578912-7 2003 PYK2 phosphorylates gelsolin at tyrosine residues and regulates gelsolin bioactivity, including decreasing gelsolin binding to actin monomer and increasing gelsolin binding to phosphatidylinositol lipids. Tyrosine 32-40 protein tyrosine kinase 2 beta Homo sapiens 0-4 12175337-3 2002 Band 3 tyrosine phosphorylation can be induced by inhibition of PTP (vanadate, thiol oxidation), activation of PTK (hypertonic NaCl) or intracellular increased Ca(2+) (mechanism unknown). Tyrosine 7-15 protein tyrosine kinase 2 beta Homo sapiens 111-114 12231407-7 2002 In calcitonin-treated osteoclast-like cells, the tyrosine phosphorylation of paxillin and FAK increased, whereas the tyrosine phosphorylation of Pyk2 decreased. Tyrosine 117-125 protein tyrosine kinase 2 beta Homo sapiens 145-149 12231407-9 2002 The unexpected dephosphorylation of Pyk2 correlated with decreased phosphorylation of Tyr(402), the autophosphorylation site of Pyk2. Tyrosine 86-89 protein tyrosine kinase 2 beta Homo sapiens 36-40 12231407-9 2002 The unexpected dephosphorylation of Pyk2 correlated with decreased phosphorylation of Tyr(402), the autophosphorylation site of Pyk2. Tyrosine 86-89 protein tyrosine kinase 2 beta Homo sapiens 128-132 12231407-11 2002 Treatment of osteoclast-like cells with 12-phorbol 13-myristate acetate increased the tyrosine phosphorylation of both Pyk2 and FAK, and calphostin C, an inhibitor of protein kinase C, blocked calcitonin-stimulated FAK phosphorylation. Tyrosine 86-94 protein tyrosine kinase 2 beta Homo sapiens 119-123 12231407-12 2002 Increasing intracellular calcium with ionomycin caused a decrease in the tyrosine phosphorylation of Pyk2 and the loss of the actin ring in a manner similar to the effect of calcitonin. Tyrosine 73-81 protein tyrosine kinase 2 beta Homo sapiens 101-105 12097497-3 2002 Activation of Pyk2 and Src/Fyn kinases led to increased tyrosine phosphorylation of NMDA receptor subunits 2A and B (NR2A/B) and was blocked by a selective mGluR1 antagonist, 7-(hydroxyamino)cyclopropa[b]chromen-1a-carboxylate ethyl ester, but not an mGluR5 antagonist, 2-methyl-6-(phenylethynyl)pyridine. Tyrosine 56-64 protein tyrosine kinase 2 beta Homo sapiens 14-18 12193071-3 2002 We have observed that Pyk2, a calcium-dependent cytoplasmic tyrosine kinase, was expressed in human renal mesangial cells and was tyrosine phosphorylated after ET-1 treatment. Tyrosine 60-68 protein tyrosine kinase 2 beta Homo sapiens 22-26 12063569-4 2002 Stimulation of human T47D breast cancer cells with HRG induced the tyrosine phosphorylation of RAFTK and its association with CHK in vitro and in vivo. Tyrosine 67-75 protein tyrosine kinase 2 beta Homo sapiens 95-100 12077257-0 2002 TCR engagement induces proline-rich tyrosine kinase-2 (Pyk2) translocation to the T cell-APC interface independently of Pyk2 activity and in an immunoreceptor tyrosine-based activation motif-mediated fashion. Tyrosine 36-44 protein tyrosine kinase 2 beta Homo sapiens 55-59 12400610-4 2002 beta1 integrin ligation induced activation of Akt, and tyrosine phosphorylation of RAFTK and FAK, but not SYK in REH cells. Tyrosine 55-63 protein tyrosine kinase 2 beta Homo sapiens 83-88 12400610-10 2002 Further evidence for a link between RAFTK tyrosine phosphorylation and Akt activation was the observation that the p85 subunit of P13 kinase associated with RAFTK following integrin ligation in REH cells. Tyrosine 42-50 protein tyrosine kinase 2 beta Homo sapiens 36-41 12097497-4 2002 Functional linkage between mGluR1 activation and NR2A tyrosine phosphorylation through Pyk2 and Src was also demonstrated after expression of these elements in human embryonic kidney 293 cells. Tyrosine 54-62 protein tyrosine kinase 2 beta Homo sapiens 87-91 12097497-6 2002 Furthermore, antagonizing calmodulin or mGluR1, but not PKC, reduced the basal tyrosine phosphorylation levels of Pyk2 and Src, suggesting that mGluR1 may control the basal activity of these kinases and thus the tyrosine phosphorylation levels of NMDA receptors. Tyrosine 79-87 protein tyrosine kinase 2 beta Homo sapiens 114-118 12097497-6 2002 Furthermore, antagonizing calmodulin or mGluR1, but not PKC, reduced the basal tyrosine phosphorylation levels of Pyk2 and Src, suggesting that mGluR1 may control the basal activity of these kinases and thus the tyrosine phosphorylation levels of NMDA receptors. Tyrosine 212-220 protein tyrosine kinase 2 beta Homo sapiens 114-118 12096713-0 2002 Activation of Pyk2/RAFTK induces tyrosine phosphorylation of alpha-synuclein via Src-family kinases. Tyrosine 33-41 protein tyrosine kinase 2 beta Homo sapiens 14-18 12096713-0 2002 Activation of Pyk2/RAFTK induces tyrosine phosphorylation of alpha-synuclein via Src-family kinases. Tyrosine 33-41 protein tyrosine kinase 2 beta Homo sapiens 19-24 12096713-2 2002 The present report demonstrates that the protein tyrosine kinase Pyk2/RAFTK is involved in cell stress-induced tyrosine phosphorylation of alpha S. Hyperosmotic stress induced tyrosine phosphorylation of alpha S via Pyk2/RAFTK at tyrosine residue 125. Tyrosine 49-57 protein tyrosine kinase 2 beta Homo sapiens 65-69 12096713-2 2002 The present report demonstrates that the protein tyrosine kinase Pyk2/RAFTK is involved in cell stress-induced tyrosine phosphorylation of alpha S. Hyperosmotic stress induced tyrosine phosphorylation of alpha S via Pyk2/RAFTK at tyrosine residue 125. Tyrosine 49-57 protein tyrosine kinase 2 beta Homo sapiens 70-75 12096713-2 2002 The present report demonstrates that the protein tyrosine kinase Pyk2/RAFTK is involved in cell stress-induced tyrosine phosphorylation of alpha S. Hyperosmotic stress induced tyrosine phosphorylation of alpha S via Pyk2/RAFTK at tyrosine residue 125. Tyrosine 49-57 protein tyrosine kinase 2 beta Homo sapiens 216-220 12096713-2 2002 The present report demonstrates that the protein tyrosine kinase Pyk2/RAFTK is involved in cell stress-induced tyrosine phosphorylation of alpha S. Hyperosmotic stress induced tyrosine phosphorylation of alpha S via Pyk2/RAFTK at tyrosine residue 125. Tyrosine 49-57 protein tyrosine kinase 2 beta Homo sapiens 221-226 12096713-2 2002 The present report demonstrates that the protein tyrosine kinase Pyk2/RAFTK is involved in cell stress-induced tyrosine phosphorylation of alpha S. Hyperosmotic stress induced tyrosine phosphorylation of alpha S via Pyk2/RAFTK at tyrosine residue 125. Tyrosine 111-119 protein tyrosine kinase 2 beta Homo sapiens 65-69 12096713-2 2002 The present report demonstrates that the protein tyrosine kinase Pyk2/RAFTK is involved in cell stress-induced tyrosine phosphorylation of alpha S. Hyperosmotic stress induced tyrosine phosphorylation of alpha S via Pyk2/RAFTK at tyrosine residue 125. Tyrosine 111-119 protein tyrosine kinase 2 beta Homo sapiens 70-75 12096713-2 2002 The present report demonstrates that the protein tyrosine kinase Pyk2/RAFTK is involved in cell stress-induced tyrosine phosphorylation of alpha S. Hyperosmotic stress induced tyrosine phosphorylation of alpha S via Pyk2/RAFTK at tyrosine residue 125. Tyrosine 111-119 protein tyrosine kinase 2 beta Homo sapiens 216-220 12096713-2 2002 The present report demonstrates that the protein tyrosine kinase Pyk2/RAFTK is involved in cell stress-induced tyrosine phosphorylation of alpha S. Hyperosmotic stress induced tyrosine phosphorylation of alpha S via Pyk2/RAFTK at tyrosine residue 125. Tyrosine 111-119 protein tyrosine kinase 2 beta Homo sapiens 221-226 12096713-2 2002 The present report demonstrates that the protein tyrosine kinase Pyk2/RAFTK is involved in cell stress-induced tyrosine phosphorylation of alpha S. Hyperosmotic stress induced tyrosine phosphorylation of alpha S via Pyk2/RAFTK at tyrosine residue 125. Tyrosine 111-119 protein tyrosine kinase 2 beta Homo sapiens 65-69 12096713-2 2002 The present report demonstrates that the protein tyrosine kinase Pyk2/RAFTK is involved in cell stress-induced tyrosine phosphorylation of alpha S. Hyperosmotic stress induced tyrosine phosphorylation of alpha S via Pyk2/RAFTK at tyrosine residue 125. Tyrosine 111-119 protein tyrosine kinase 2 beta Homo sapiens 70-75 12096713-2 2002 The present report demonstrates that the protein tyrosine kinase Pyk2/RAFTK is involved in cell stress-induced tyrosine phosphorylation of alpha S. Hyperosmotic stress induced tyrosine phosphorylation of alpha S via Pyk2/RAFTK at tyrosine residue 125. Tyrosine 111-119 protein tyrosine kinase 2 beta Homo sapiens 216-220 12096713-2 2002 The present report demonstrates that the protein tyrosine kinase Pyk2/RAFTK is involved in cell stress-induced tyrosine phosphorylation of alpha S. Hyperosmotic stress induced tyrosine phosphorylation of alpha S via Pyk2/RAFTK at tyrosine residue 125. Tyrosine 111-119 protein tyrosine kinase 2 beta Homo sapiens 221-226 12096713-5 2002 Accordingly, such results indicate that Pyk2/RAFTK lies upstream of Src-family kinases in the signaling cascade by which osmotic stress induces tyrosine phosphorylation of alpha S. Tyrosine 144-152 protein tyrosine kinase 2 beta Homo sapiens 40-44 12096713-5 2002 Accordingly, such results indicate that Pyk2/RAFTK lies upstream of Src-family kinases in the signaling cascade by which osmotic stress induces tyrosine phosphorylation of alpha S. Tyrosine 144-152 protein tyrosine kinase 2 beta Homo sapiens 45-50 11856738-4 2002 This inactivation may result from the direct phosphorylation of ARA55 by Pyk2 at tyrosine 43, impairing the coactivator activity of ARA55 and/or sequestering ARA55 to reduce its interaction with AR. Tyrosine 81-89 protein tyrosine kinase 2 beta Homo sapiens 73-77 11916084-6 2002 By contrast, tyrosine phosphorylation of Pyk2 was only partially reduced by aspirin and RGDS, and was not affected by either calcium chelation or PKC inhibition, suggesting that activation of this kinase does not require phospholipase-mediated signalling. Tyrosine 13-21 protein tyrosine kinase 2 beta Homo sapiens 41-45 11916084-8 2002 Prevention of cytoskeletal relocation of Pyk2 and FAK by cytochalasin D totally blocked vWF-induced tyrosine phosphorylation of both kinases. Tyrosine 100-108 protein tyrosine kinase 2 beta Homo sapiens 41-45 11581189-8 2001 Furthermore, tyrosine phosphorylation of FAK and phosphotyrosine kinase (Pyk) 2; serine phosphorylation of c-Raf, MEK1, and Elk 1; and tyrosine-threonine phosphorylation of Erk-1 and -2 were time-dependently activated in the presence of GDNF. Tyrosine 13-21 protein tyrosine kinase 2 beta Homo sapiens 49-79 11839685-9 2002 Some of these tyrosine-phosphorylated bands were identified as the focal adhesion proteins paxillin, FAK, PYK2, and the c-Met receptor itself. Tyrosine 14-22 protein tyrosine kinase 2 beta Homo sapiens 106-110 11782427-2 2002 This represents phosphorylation by the serine/threonine kinase protein kinase B (PKB), since PKB constitutively associates with Gab2, phosphorylates Gab2 on a consensus phosphorylation site, Ser159, in vitro and inhibits Gab2 tyrosine phosphorylation. Tyrosine 226-234 protein tyrosine kinase 2 beta Homo sapiens 63-79 11782427-2 2002 This represents phosphorylation by the serine/threonine kinase protein kinase B (PKB), since PKB constitutively associates with Gab2, phosphorylates Gab2 on a consensus phosphorylation site, Ser159, in vitro and inhibits Gab2 tyrosine phosphorylation. Tyrosine 226-234 protein tyrosine kinase 2 beta Homo sapiens 81-84 11782427-2 2002 This represents phosphorylation by the serine/threonine kinase protein kinase B (PKB), since PKB constitutively associates with Gab2, phosphorylates Gab2 on a consensus phosphorylation site, Ser159, in vitro and inhibits Gab2 tyrosine phosphorylation. Tyrosine 226-234 protein tyrosine kinase 2 beta Homo sapiens 93-96 11602570-3 2001 Here we report the identification of two proteins selectively tyrosine-phosphorylated in response to ET-1 but not insulin: the Ca(2+)-activated tyrosine kinase PYK2 and its physiological substrate, the adhesion scaffold protein paxillin. Tyrosine 62-70 protein tyrosine kinase 2 beta Homo sapiens 160-164 11602570-5 2001 CADTK-related non-kinase (CRNK) is a dominant negative form of PYK2 containing the C-terminal portion of the protein, which binds paxillin but lacks the PYK2 autophosphorylation site (Tyr(402)). Tyrosine 184-187 protein tyrosine kinase 2 beta Homo sapiens 63-67 11520052-6 2001 In this regard, K457A suppressed PYK2 kinase activation by AngII, whereas it enhanced AngII-induced PYK2 Tyr(402) phosphorylation. Tyrosine 105-108 protein tyrosine kinase 2 beta Homo sapiens 100-104 11520052-7 2001 By contrast, Y402F inhibited PYK2 Tyr(402) phosphorylation, whereas it markedly enhanced AngII-induced PYK2 kinase activation. Tyrosine 34-37 protein tyrosine kinase 2 beta Homo sapiens 29-33 11478917-2 2001 Here we show that acrylamide induces morphological changes and tyrosine phosphorylation of focal adhesion kinase (FAK) and proline-rich tyrosine kinase 2 (Pyk2), a member of the FAK subfamily, in human differentiating neuroblastoma SH-SY5Y cells. Tyrosine 63-71 protein tyrosine kinase 2 beta Homo sapiens 155-159 11493697-5 2001 Expression of nephrocystin resulted in phosphorylation of Pyk2 on tyrosine 402 as well as activation of downstream mitogen-activated protein kinases, such as ERK1 and ERK2. Tyrosine 66-74 protein tyrosine kinase 2 beta Homo sapiens 58-62 11478917-5 2001 The compound also effectively inhibited the biological and biochemical responses caused by another axonopathy inducer, colchicine, including tyrosine phosphorylation of Pyk2, formation of an 85-kDa poly(ADP-ribose)polymerase (PARP) fragment and apoptosis-associated induction of the NAPOR gene as well as neuronal cell death. Tyrosine 141-149 protein tyrosine kinase 2 beta Homo sapiens 169-173 11467832-5 2001 Further, forskolin increased phosphorylation of protein tyrosine, suggesting that cAMP activates PTK. Tyrosine 56-64 protein tyrosine kinase 2 beta Homo sapiens 97-100 11461120-6 2001 In addition stimulation with MIP-1alpha induces tyrosine phosphorylation of the proline-rich tyrosine kinase Pyk-2. Tyrosine 48-56 protein tyrosine kinase 2 beta Homo sapiens 109-114 11337490-2 2001 Focal adhesion kinase (FAK) and cell adhesion kinase beta (CAKbeta)/PYK2/CADTK/RAFTK are protein-tyrosine kinases that can colocalize with, bind to, and induce tyrosine phosphorylation of p130(CAS) and paxillin. Tyrosine 97-105 protein tyrosine kinase 2 beta Homo sapiens 32-57 11337490-2 2001 Focal adhesion kinase (FAK) and cell adhesion kinase beta (CAKbeta)/PYK2/CADTK/RAFTK are protein-tyrosine kinases that can colocalize with, bind to, and induce tyrosine phosphorylation of p130(CAS) and paxillin. Tyrosine 97-105 protein tyrosine kinase 2 beta Homo sapiens 59-66 11337490-2 2001 Focal adhesion kinase (FAK) and cell adhesion kinase beta (CAKbeta)/PYK2/CADTK/RAFTK are protein-tyrosine kinases that can colocalize with, bind to, and induce tyrosine phosphorylation of p130(CAS) and paxillin. Tyrosine 97-105 protein tyrosine kinase 2 beta Homo sapiens 68-72 11337490-2 2001 Focal adhesion kinase (FAK) and cell adhesion kinase beta (CAKbeta)/PYK2/CADTK/RAFTK are protein-tyrosine kinases that can colocalize with, bind to, and induce tyrosine phosphorylation of p130(CAS) and paxillin. Tyrosine 97-105 protein tyrosine kinase 2 beta Homo sapiens 73-78 11337490-2 2001 Focal adhesion kinase (FAK) and cell adhesion kinase beta (CAKbeta)/PYK2/CADTK/RAFTK are protein-tyrosine kinases that can colocalize with, bind to, and induce tyrosine phosphorylation of p130(CAS) and paxillin. Tyrosine 97-105 protein tyrosine kinase 2 beta Homo sapiens 79-84 11337490-5 2001 Both the major autophosphorylation site of CAKbeta (Tyr(402)) and activation loop tyrosine residues, Tyr(579) and Tyr(580), were targeted for dephosphorylation by PTP-PEST. Tyrosine 52-55 protein tyrosine kinase 2 beta Homo sapiens 43-50 11337490-8 2001 Tyrosine phosphorylation of paxillin, which is greatly enhanced by CAKbeta overexpression, was dramatically reduced upon coexpression of PTP-PEST. Tyrosine 0-8 protein tyrosine kinase 2 beta Homo sapiens 67-74 11352836-9 2001 AVP treatment appeared to transactivate EGF-R by inducing tyrosine phosphorylation of the Ca(2+)/protein kinase C (PKC)-dependent nonreceptor tyrosine kinase, Pyk2, leading to Pyk2/c-Src association and c-Src activation. Tyrosine 58-66 protein tyrosine kinase 2 beta Homo sapiens 159-163 11278444-4 2001 We have observed that Pyk2 was expressed in HMC and was tyrosine-phosphorylated within 5 min of ET-1 treatment. Tyrosine 56-64 protein tyrosine kinase 2 beta Homo sapiens 22-26 11352836-9 2001 AVP treatment appeared to transactivate EGF-R by inducing tyrosine phosphorylation of the Ca(2+)/protein kinase C (PKC)-dependent nonreceptor tyrosine kinase, Pyk2, leading to Pyk2/c-Src association and c-Src activation. Tyrosine 58-66 protein tyrosine kinase 2 beta Homo sapiens 176-180 11352836-11 2001 These data suggested that AVP-stimulated Pyk2 tyrosine phosphorylation to activate c-Src, thereby leading to EGF-R transactivation. Tyrosine 46-54 protein tyrosine kinase 2 beta Homo sapiens 41-45 11311138-4 2001 Pyk2 was tyrosine phosphorylated very quickly after stimulation with MCP-1. Tyrosine 9-17 protein tyrosine kinase 2 beta Homo sapiens 0-4 11311138-7 2001 The association of Pyk2 with Lyn was dependent on stimulation with MCP-1 and on tyrosine phosphorylation of Pyk2. Tyrosine 80-88 protein tyrosine kinase 2 beta Homo sapiens 19-23 11311138-7 2001 The association of Pyk2 with Lyn was dependent on stimulation with MCP-1 and on tyrosine phosphorylation of Pyk2. Tyrosine 80-88 protein tyrosine kinase 2 beta Homo sapiens 108-112 11311138-8 2001 Phosphorylation of p38 was also dependent on tyrosine phosphorylation of Pyk2. Tyrosine 45-53 protein tyrosine kinase 2 beta Homo sapiens 73-77 21965067-6 2001 Alternatively, antibodies recognizing phosphorylated tyrosine residues can be used to quantify PTK activity. Tyrosine 53-61 protein tyrosine kinase 2 beta Homo sapiens 95-98 11036077-5 2001 In addition, biochemical and pharmacological analysis revealed that CD146 engagement initiates the tyrosine phosphorylation of phospholipase C-gamma, Pyk2, and p130(Cas). Tyrosine 99-107 protein tyrosine kinase 2 beta Homo sapiens 150-154 11238916-11 2001 The results suggest that paxillin is dephosphorylated by PTP phi in dorsal ruffles, using Pyk2 as a bridging molecule, resulting in a reduced pool of tyrosine-phosphorylated paxillin available for incorporation into focal complexes, thereby mediating CSF-1 regulation of macrophage morphology, adhesion, and motility. Tyrosine 150-158 protein tyrosine kinase 2 beta Homo sapiens 90-94 11239437-5 2001 Tyrosine phosphorylation of CAKbeta/Pyk2 and its association with Src was increased by stimulation that produced LTP. Tyrosine 0-8 protein tyrosine kinase 2 beta Homo sapiens 28-35 11239437-5 2001 Tyrosine phosphorylation of CAKbeta/Pyk2 and its association with Src was increased by stimulation that produced LTP. Tyrosine 0-8 protein tyrosine kinase 2 beta Homo sapiens 36-40 11036077-8 2001 These results indicate that CD146 is coupled to a FYN-dependent pathway that triggers Ca(2+) flux via phospholipase C-gamma activation leading subsequently to the tyrosine phosphorylation of downstream targets such as Pyk2, p130(Cas), FAK, and paxillin. Tyrosine 163-171 protein tyrosine kinase 2 beta Homo sapiens 218-222 10915788-5 2000 Translocation of PYK2 to focal adhesions, as well as its tyrosine phosphorylation in response to histamine treatment, was abolished in the presence of protein kinase C inhibitors or cytochalasin D treatment, whereas activation of protein kinase C by phorbol ester resulted in focal adhesion targeting of PYK2 and its tyrosine phosphorylation in an integrin-clustering dependent manner. Tyrosine 317-325 protein tyrosine kinase 2 beta Homo sapiens 17-21 11204274-3 2001 Pyk2 is rapidly tyrosine phosphorylated in response to diverse extracellular signals acting via different post receptor pathways. Tyrosine 16-24 protein tyrosine kinase 2 beta Homo sapiens 0-4 11007796-6 2000 Glucose stimulated the phosphorylation of tyrosine residues 402 and 881 in PYK2 and binding of PYK2 to Myc-Glut1. Tyrosine 42-50 protein tyrosine kinase 2 beta Homo sapiens 75-79 10915788-4 2000 Translocation of PYK2 to focal adhesions was attributed to enhanced tyrosine phosphorylation of PYK2 and its association with the focal adhesion proteins paxillin and p130(Cas). Tyrosine 68-76 protein tyrosine kinase 2 beta Homo sapiens 17-21 10961871-7 2000 In vitro kinase assays revealed that Pyk2 and Syk represented kinase activities to induce tyrosine phosphorylation of several molecules in the anti-beta2 integrin immunoprecipitates of the attached cells. Tyrosine 90-98 protein tyrosine kinase 2 beta Homo sapiens 37-41 10915788-4 2000 Translocation of PYK2 to focal adhesions was attributed to enhanced tyrosine phosphorylation of PYK2 and its association with the focal adhesion proteins paxillin and p130(Cas). Tyrosine 68-76 protein tyrosine kinase 2 beta Homo sapiens 96-100 10915788-5 2000 Translocation of PYK2 to focal adhesions, as well as its tyrosine phosphorylation in response to histamine treatment, was abolished in the presence of protein kinase C inhibitors or cytochalasin D treatment, whereas activation of protein kinase C by phorbol ester resulted in focal adhesion targeting of PYK2 and its tyrosine phosphorylation in an integrin-clustering dependent manner. Tyrosine 57-65 protein tyrosine kinase 2 beta Homo sapiens 17-21 10880513-6 2000 We demonstrate that RAFTK is a direct substrate of SHP2 both in vitro and in vivo, and that Tyr(906) in the C-terminal domain of RAFTK mediates its interaction with SHP2. Tyrosine 92-95 protein tyrosine kinase 2 beta Homo sapiens 129-134 10961871-3 2000 When the granulocytic cells were stimulated with N-formyl-L-methionyl-L-leucyl-L-phenylalanine (fMLP), tyrosine phosphorylation of Pyk2 occurred promptly and subsequent association of Pyk2 with beta2 integrin was detected. Tyrosine 103-111 protein tyrosine kinase 2 beta Homo sapiens 131-135 10934044-10 2000 Interestingly, both Pyk2 and FPhy2 (to a greater extent) were tyrosine phosphorylated and associated with Src and Fyn. Tyrosine 62-70 protein tyrosine kinase 2 beta Homo sapiens 20-24 10961871-6 2000 Cell attachment to fibrinogen caused the enhanced tyrosine phosphorylation of Pyk2 and the initial tyrosine phosphorylation of Syk, which was also inhibited by pretreatment with anti-beta2 integrin antibody. Tyrosine 50-58 protein tyrosine kinase 2 beta Homo sapiens 78-82 10867021-0 2000 Protein-tyrosine kinase Pyk2 is involved in interleukin-2 production by Jurkat T cells via its tyrosine 402. Tyrosine 8-16 protein tyrosine kinase 2 beta Homo sapiens 24-28 10867021-2 2000 Pyk2 as well as kinase-inactive Pyk2-K457A, was phosphorylated at tyrosine residues 402, 580, and 881 upon T cell antigen receptor cross-linking, indicating that these residues are phosphorylated by other tyrosine kinase(s). Tyrosine 66-74 protein tyrosine kinase 2 beta Homo sapiens 0-4 10867021-2 2000 Pyk2 as well as kinase-inactive Pyk2-K457A, was phosphorylated at tyrosine residues 402, 580, and 881 upon T cell antigen receptor cross-linking, indicating that these residues are phosphorylated by other tyrosine kinase(s). Tyrosine 66-74 protein tyrosine kinase 2 beta Homo sapiens 32-36 10867021-3 2000 However, no tyrosine phosphorylation of Pyk2-Y402F was detected while more than 60% of the tyrosine phosphorylation was observed in Pyk2-Y881F. Tyrosine 91-99 protein tyrosine kinase 2 beta Homo sapiens 132-136 10867021-8 2000 Taken together, these findings suggest that Pyk2 is involved in the activation of T cells through its tyrosine 402. Tyrosine 102-110 protein tyrosine kinase 2 beta Homo sapiens 44-48 10749687-4 2000 The residual Pyk2 tyrosine phosphorylation observed in the absence of platelet aggregation was completely abolished by pretreatment with BAPTA/AM [bis-(o-aminophenoxy)ethane-N,N,N",N"-tetra-acetic acid acetoxymethyl ester]. Tyrosine 18-26 protein tyrosine kinase 2 beta Homo sapiens 13-17 10797305-2 2000 We show that PYK2 is tyrosine phosphorylated and its activity is increased during early stages of platelet aggregation. Tyrosine 21-29 protein tyrosine kinase 2 beta Homo sapiens 13-17 10797305-8 2000 These results support a model in which PYK2 is associated with PI 3-kinase in unstimulated platelets and following activation of platelets, there is an increase in tyrosine phosphorylation of PYK2, increased PYK2 activity, and increased association of PYK2 with PI 3-kinase, which may contribute to the increase in PI 3-kinase activity. Tyrosine 164-172 protein tyrosine kinase 2 beta Homo sapiens 39-43 10797305-8 2000 These results support a model in which PYK2 is associated with PI 3-kinase in unstimulated platelets and following activation of platelets, there is an increase in tyrosine phosphorylation of PYK2, increased PYK2 activity, and increased association of PYK2 with PI 3-kinase, which may contribute to the increase in PI 3-kinase activity. Tyrosine 164-172 protein tyrosine kinase 2 beta Homo sapiens 192-196 10797305-8 2000 These results support a model in which PYK2 is associated with PI 3-kinase in unstimulated platelets and following activation of platelets, there is an increase in tyrosine phosphorylation of PYK2, increased PYK2 activity, and increased association of PYK2 with PI 3-kinase, which may contribute to the increase in PI 3-kinase activity. Tyrosine 164-172 protein tyrosine kinase 2 beta Homo sapiens 192-196 10797305-8 2000 These results support a model in which PYK2 is associated with PI 3-kinase in unstimulated platelets and following activation of platelets, there is an increase in tyrosine phosphorylation of PYK2, increased PYK2 activity, and increased association of PYK2 with PI 3-kinase, which may contribute to the increase in PI 3-kinase activity. Tyrosine 164-172 protein tyrosine kinase 2 beta Homo sapiens 192-196 10749687-3 2000 When platelets were stimulated with thrombin, Pyk2, as well as FAK, was markedly tyrosine-phosphorylated, in a manner mostly dependent on alphaIIbbeta3 integrin-mediated aggregation. Tyrosine 81-89 protein tyrosine kinase 2 beta Homo sapiens 46-50 10800936-4 2000 We found that the tyrosine kinase related adhesion focal tyrosine kinase (RAFTK) is tyrosine phosphorylated in response to glutamate in a time- and dose-dependent manner. Tyrosine 18-26 protein tyrosine kinase 2 beta Homo sapiens 34-72 10800936-4 2000 We found that the tyrosine kinase related adhesion focal tyrosine kinase (RAFTK) is tyrosine phosphorylated in response to glutamate in a time- and dose-dependent manner. Tyrosine 18-26 protein tyrosine kinase 2 beta Homo sapiens 74-79 10800936-6 2000 RAFTK tyrosine phosphorylation was mediated primarily by class I/II metabotropic glutamate receptors and depends on protein kinase C (PKC) activation. Tyrosine 6-14 protein tyrosine kinase 2 beta Homo sapiens 0-5 10753828-3 2000 We previously characterized signal transduction pathways induced by SDF-1alpha in human hematopoietic progenitors and found tyrosine phosphorylation of focal adhesion components, including the related adhesion focal tyrosine kinase (RAFTK), the adaptor molecule p130 Cas, and the cytoskeletal protein paxillin. Tyrosine 124-132 protein tyrosine kinase 2 beta Homo sapiens 193-231 10749687-6 2000 In contrast, direct activation of PKC with the active phorbol ester PMA induced the tyrosine phosphorylation of Pyk2 and FAK but only when platelets were fully aggregated with the exogenous addition of fibrinogen (the ligand for alphaIIbbeta3 integrin). Tyrosine 84-92 protein tyrosine kinase 2 beta Homo sapiens 112-116 10749687-7 2000 Furthermore, PMA-induced Pyk2 (and FAK) tyrosine phosphorylation was also observed when platelets adhered to immobilized fibrinogen. Tyrosine 40-48 protein tyrosine kinase 2 beta Homo sapiens 25-29 10749687-13 2000 Although Pyk2 and FAK have been reported to be differentially regulated in many cell types, our results suggest that, in human platelets, the mode of Pyk2 activation is mostly similar to that of FAK, in terms of alphaIIbbeta3 integrin-dependent and PKC-dependent tyrosine phosphorylation. Tyrosine 263-271 protein tyrosine kinase 2 beta Homo sapiens 150-154 10749687-14 2000 Furthermore, Pyk2, as well as FAK, might have one or more important roles in post-aggregation tyrosine phosphorylation events, in association with the cytoskeleton and through interaction with adapter proteins including Grb2 and Shc. Tyrosine 94-102 protein tyrosine kinase 2 beta Homo sapiens 13-17 10675474-2 2000 The novel protein tyrosine inhibitor PTK 787 was evaluated in two models of human ovarian cancer: Hey-A8 cells, which express low levels of VEGF/VPF and grow as solid tumor foci on the surface of peritoneal organs, and SKOV3 i.p.1 cells, which express high levels of VEGF/VPF and grow as solid peritoneal tumors and ascites. Tyrosine 18-26 protein tyrosine kinase 2 beta Homo sapiens 37-40 10722671-4 2000 We found that the Ang II-induced tyrosine phosphorylation of Pyk2, which requires the activity of Src family kinase, was specifically regulated by the Src family kinase member, Yes kinase. Tyrosine 33-41 protein tyrosine kinase 2 beta Homo sapiens 61-65 10722671-10 2000 Thus, the balance of Pyk2 tyrosine phosphorylation in response to Ang II is controlled by Yes kinase and by a tyrosine phosphatase SHP-2 in endothelial cells. Tyrosine 26-34 protein tyrosine kinase 2 beta Homo sapiens 21-25 10713673-4 2000 Stimulation of T47D cells with heregulin (HRG) induced the tyrosine phosphorylation of RAFTK and the formation of a multiprotein complex. Tyrosine 59-67 protein tyrosine kinase 2 beta Homo sapiens 87-92 10713673-5 2000 Analyses of the members of the HRG-stimulated complex revealed that RAFTK is associated with p190 RhoGAP (p190), RasGAP and ErbB-2, and plays an essential role in mediating the tyrosine phosphorylation of p190 by Src. Tyrosine 177-185 protein tyrosine kinase 2 beta Homo sapiens 68-73 10614772-11 1999 Treatment of human monocytes with the peptide stimulates tyrosine phosphorylation of several cellular proteins, including p125FAK and Pyk2 and translocation of RhoA from the cytosol to the membrane. Tyrosine 57-65 protein tyrosine kinase 2 beta Homo sapiens 134-138 10625683-0 2000 Regulation of T cell receptor- and CD28-induced tyrosine phosphorylation of the focal adhesion tyrosine kinases Pyk2 and Fak by protein kinase C. A role for protein tyrosine phosphatases. Tyrosine 48-56 protein tyrosine kinase 2 beta Homo sapiens 112-116 10709848-2 2000 Adhesion of tonsil B cells in our system induced tyrosine phosphorylation of Pyk-2. Tyrosine 49-57 protein tyrosine kinase 2 beta Homo sapiens 77-82 10569699-8 1999 Our results suggest that tyrosine phosphorylation of a protein with molecular mass of about 25 kD via Src-family PTK(s) is an early event in T-lymphocyte activation associated with the homotypic aggregation in response to RANTES. Tyrosine 25-33 protein tyrosine kinase 2 beta Homo sapiens 113-116 10542211-7 1999 KSHV-GPCR induced the tyrosine phosphorylation of RAFTK. Tyrosine 22-30 protein tyrosine kinase 2 beta Homo sapiens 50-55 10518561-6 1999 In 293 cells expressing recombinant BRDG1 and various PTKs, Tec and Pyk2, but not Btk, Bmx, Lyn, Syk, or c-Abl, induced marked phosphorylation of BRDG1 on tyrosine residues. Tyrosine 155-163 protein tyrosine kinase 2 beta Homo sapiens 68-72 10430614-2 1999 Here we identify pyk2 in primary human neutrophils; localize it to focal adhesions and podosomes; and demonstrate its tyrosine phosphorylation, activation, and association with paxillin during stimulation of adherent cells by TNF. Tyrosine 118-126 protein tyrosine kinase 2 beta Homo sapiens 17-21 10497255-5 1999 Overexpression of PKB did not affect the acute Tyr phosphorylation of IRS-1; however, it significantly attenuated its rate of Tyr dephosphorylation following 60 min of treatment with insulin. Tyrosine 126-129 protein tyrosine kinase 2 beta Homo sapiens 18-21 10497255-8 1999 In contrast, insulin-stimulated PKB-mediated phosphorylation of Ser residues within the phosphotyrosine binding/SAIN domain of IRS-1 protects IRS-1 from the rapid action of protein-tyrosine phosphatases and enables it to maintain its Tyr-phosphorylated active conformation. Tyrosine 234-237 protein tyrosine kinase 2 beta Homo sapiens 32-35 10430614-4 1999 Tyrphostin A9 inhibited TNF-induced tyrosine phosphorylation of pyk2 without blocking the cells" bactericidal activity. Tyrosine 36-44 protein tyrosine kinase 2 beta Homo sapiens 64-68 10430614-5 1999 Wortmannin, an inhibitor of phosphatidylinositol-3-kinase, potently blocked the TNF-induced respiratory burst and selectively inhibited tyrosine phosphorylation of pyk2. Tyrosine 136-144 protein tyrosine kinase 2 beta Homo sapiens 164-168 10362354-7 1999 Tyrosine 577 and 612 were found to be critical for the activation of PKB and ERK1, but not activation of STAT transcription factors. Tyrosine 0-8 protein tyrosine kinase 2 beta Homo sapiens 69-72 10358164-5 1999 The block of ERK activation is exerted at an early, PTK-dependent stage in the events leading to p21ras activation, as the CD16-induced tyrosine phosphorylation of Shc adaptor protein and the formation of Shc/Grb-2 complex are abrogated by CD94/NKG2-A simultaneous engagement. Tyrosine 136-144 protein tyrosine kinase 2 beta Homo sapiens 52-55 10356363-0 1999 Human glomerular epithelial cell express CD4 and interaction with gp120 protein promotes PYK2 tyrosine phosphorylation. Tyrosine 94-102 protein tyrosine kinase 2 beta Homo sapiens 89-93 10356979-4 1999 Tyrosine phosphorylation of Pyk2 was attenuated by beta2 integrin blocking with specific antibodies. Tyrosine 0-8 protein tyrosine kinase 2 beta Homo sapiens 28-32 10356979-5 1999 The tyrosine phosphorylation of Pyk2 was downstream of protein kinases Lyn, Syk and protein kinase C and cytoskeletal organization. Tyrosine 4-12 protein tyrosine kinase 2 beta Homo sapiens 32-36 10356363-12 1999 gp120 protein promoted vGEC tyrosine phosphorylation of pyk2 in a dose- and time-dependent manner. Tyrosine 28-36 protein tyrosine kinase 2 beta Homo sapiens 56-60 10085136-6 1999 Src family tyrosine kinases resulted in CADTK tyrosine phosphorylation even when co-expressed with the Tyr402/Tyr881 double mutant, suggesting that Src/Fyn etc. Tyrosine 11-19 protein tyrosine kinase 2 beta Homo sapiens 40-45 10085098-9 1999 These findings indicate that RAFTK-dependent induction of JNK in response to MMS is sensitive to Bcl-xL, but not to CrmA and p35, by a mechanism that inhibits tyrosine phosphorylation and thereby activation of RAFTK. Tyrosine 159-167 protein tyrosine kinase 2 beta Homo sapiens 29-34 10085098-9 1999 These findings indicate that RAFTK-dependent induction of JNK in response to MMS is sensitive to Bcl-xL, but not to CrmA and p35, by a mechanism that inhibits tyrosine phosphorylation and thereby activation of RAFTK. Tyrosine 159-167 protein tyrosine kinase 2 beta Homo sapiens 210-215 10085136-8 1999 Interestingly, CADTK tyrosine-phosphorylated FAK when both were transiently expressed, but FAK did not phosphorylate CADTK. Tyrosine 21-29 protein tyrosine kinase 2 beta Homo sapiens 15-20 10085136-10 1999 This phosphorylation utilized tyrosine residues other than Tyr397, Tyr925, or Tyr576/Tyr577, suggesting that new SH2-binding sites might be created by CADTK-dependent FAK phosphorylation. Tyrosine 30-38 protein tyrosine kinase 2 beta Homo sapiens 151-156 10093970-0 1999 Suppression of protein kinase C is associated with inhibition of PYK2 tyrosine phosphorylation and enhancement of PYK2 interaction with Src in thrombin-activated platelets. Tyrosine 70-78 protein tyrosine kinase 2 beta Homo sapiens 65-69 10087274-9 1999 However, both the tyrosine phosphorylation of insulin receptor substrate-1 and its association with phosphatidylinositol 3-kinase were enhanced in cells cultured on BM, as was the phosphorylation of the phosphatidylinositol 3-kinase effector, protein kinase B. Tyrosine 18-26 protein tyrosine kinase 2 beta Homo sapiens 243-259 10093970-3 1999 While PYK2 tyrosine phosphorylation induced by thrombin was inhibited by preincubation of platelets with PKC inhibitors, staurosporine and Ro31-8220, PYK2 association with Src was markedly enhanced under the same conditions. Tyrosine 11-19 protein tyrosine kinase 2 beta Homo sapiens 6-10 10093970-3 1999 While PYK2 tyrosine phosphorylation induced by thrombin was inhibited by preincubation of platelets with PKC inhibitors, staurosporine and Ro31-8220, PYK2 association with Src was markedly enhanced under the same conditions. Tyrosine 11-19 protein tyrosine kinase 2 beta Homo sapiens 150-154 10022914-7 1999 Activation of PYK2 by agents that elevate intracellular calcium or by phorbol ester induce tyrosine phosphorylation of Nirs. Tyrosine 91-99 protein tyrosine kinase 2 beta Homo sapiens 14-18 10037772-3 1999 We found that ligating CD28 in Jurkat T cells rapidly increases the tyrosine phosphorylation of Pyk2 but not of Fak. Tyrosine 68-76 protein tyrosine kinase 2 beta Homo sapiens 96-100 10037772-5 1999 CD28-induced tyrosine phosphorylation of Pyk2 was markedly reduced in the absence of external Ca2+. Tyrosine 13-21 protein tyrosine kinase 2 beta Homo sapiens 41-45 10037772-6 1999 Previous studies have shown that the T cell antigen receptor (TCR) induces tyrosine phosphorylation of Pyk2. Tyrosine 75-83 protein tyrosine kinase 2 beta Homo sapiens 103-107 10037772-7 1999 In this report, the concurrent ligation of CD28 and TCR increased tyrosine phosphorylation of Pyk2; however, the extent of phosphorylation by both receptors was equivalent to the sum of that induced by each receptor alone. Tyrosine 66-74 protein tyrosine kinase 2 beta Homo sapiens 94-98 10037772-8 1999 The Syk/Zap inhibitor piceatannol blocked CD28, and TCR induced tyrosine phosphorylation of Pyk2, suggesting that Syk/Zap is involved in Pyk2 phosphorylation. Tyrosine 64-72 protein tyrosine kinase 2 beta Homo sapiens 92-96 10037772-8 1999 The Syk/Zap inhibitor piceatannol blocked CD28, and TCR induced tyrosine phosphorylation of Pyk2, suggesting that Syk/Zap is involved in Pyk2 phosphorylation. Tyrosine 64-72 protein tyrosine kinase 2 beta Homo sapiens 137-141 10022920-4 1999 We demonstrate that Pap forms a stable complex with Pyk2 and that activation of Pyk2 leads to tyrosine phosphorylation of Pap in living cells. Tyrosine 94-102 protein tyrosine kinase 2 beta Homo sapiens 52-56 10022920-4 1999 We demonstrate that Pap forms a stable complex with Pyk2 and that activation of Pyk2 leads to tyrosine phosphorylation of Pap in living cells. Tyrosine 94-102 protein tyrosine kinase 2 beta Homo sapiens 80-84 10037772-0 1999 CD28 ligation induces tyrosine phosphorylation of Pyk2 but not Fak in Jurkat T cells. Tyrosine 22-30 protein tyrosine kinase 2 beta Homo sapiens 50-54 9545257-7 1998 In contrast, monocyte adherence to tissue culture plastic-stimulated CADTK tyrosine phosphorylation, a process that was enhanced by thapsigargin, phorbol 12-myristate 13-acetate, and RANTES but that was completely blocked by preincubation with cytochalasin D. Tyrosine 75-83 protein tyrosine kinase 2 beta Homo sapiens 69-74 10706243-4 1999 Our studies previously demonstrated that the rapid increase in intracellular Ca2+ produced by DMBA in HPB-ALL T cells was caused by protein tyrosine kinase (PTK) activation in human T cells, leading to tyrosine phosphorylation of phospholipase C (PLCgamma) and IP3-dependent Ca2+ mobilization. Tyrosine 140-148 protein tyrosine kinase 2 beta Homo sapiens 157-160 9774361-9 1998 Overexpression of the dominant negative mutant of Pyk2 significantly attenuated Ang II or A23187-induced ERK activities (36% and 38% inhibition compared with that in mock-transfected cells, respectively) and ERK tyrosine phosphorylation levels, as well as an increase in the binding of GTP to p21(Ras). Tyrosine 212-220 protein tyrosine kinase 2 beta Homo sapiens 50-54 9682033-9 1998 More recently, ligand-engagement or clustering of alpha v beta 3 integrins in osteoclasts induced tyrosine phosphorylation of PYK2, a member of the focal adhesion kinase family, and of p130cas, a substrate of v-Src and v-Crk. Tyrosine 98-106 protein tyrosine kinase 2 beta Homo sapiens 126-130 9619586-4 1998 When treated with genistein (a protein tyrosine kinase PTK inhibitor), the enhanced tyrosine phosphorylation/activation of the kinase in PC-3 cells can be blocked. Tyrosine 39-47 protein tyrosine kinase 2 beta Homo sapiens 55-58 9560226-3 1998 We find that PYK2 tyrosine kinase activity is critical for the m1 receptor-stimulated tyrosine phosphorylation of PYK2. Tyrosine 18-26 protein tyrosine kinase 2 beta Homo sapiens 13-17 9560226-3 1998 We find that PYK2 tyrosine kinase activity is critical for the m1 receptor-stimulated tyrosine phosphorylation of PYK2. Tyrosine 18-26 protein tyrosine kinase 2 beta Homo sapiens 114-118 9560226-4 1998 Furthermore, we identify two tyrosine residues that are subject to phosphorylation in response to muscarinic signaling and show that this phosphorylation induces two cytosolic proteins, c-Src and Grb2, to bind to PYK2. Tyrosine 29-37 protein tyrosine kinase 2 beta Homo sapiens 213-217 9560226-5 1998 This is the first demonstration of the significance played by distinct PYK2 tyrosine residues in G protein-coupled signaling to this kinase. Tyrosine 76-84 protein tyrosine kinase 2 beta Homo sapiens 71-75 9842930-3 1998 Stimulation of Jurkat T cells with the alpha4beta1 integrin ligand VCAM-1 results in Pyk2 tyrosine phosphorylation, and combined stimulation with VCAM-1 and anti-CD3 mAb induces rapid and sustained synergistic Pyk2 phosphorylation. Tyrosine 90-98 protein tyrosine kinase 2 beta Homo sapiens 85-89 9842930-4 1998 Studies with mAb suggest that in synergistic CD3- and alpha4beta1 integrin-mediated Pyk2 tyrosine phosphorylation, a major contribution of CD3-derived signals is independent of their effects on regulating integrin adhesion. Tyrosine 89-97 protein tyrosine kinase 2 beta Homo sapiens 84-88 9842930-5 1998 Analysis of resting human CD4+ T cells confirmed the ability of CD3-derived signals to synergize with beta1 integrin-dependent signals in the induction of Pyk2 tyrosine phosphorylation. Tyrosine 160-168 protein tyrosine kinase 2 beta Homo sapiens 155-159 9796924-6 1998 Triggering of sIg induced, within seconds, identical tyrosine phosphorylation of p53/56lyn protein tyrosine kinase (PTK) and p55blk PTK in both of the cell lines; however, a prominent tyrosine phosphorylation and activation of p72syk PTK only in HF-1.3.4 cells. Tyrosine 53-61 protein tyrosine kinase 2 beta Homo sapiens 116-119 9722546-4 1998 There was enhanced tyrosine phosphorylation and association of components of focal adhesion complexes such as the related adhesion focal tyrosine kinase, paxillin, and Crk. Tyrosine 19-27 protein tyrosine kinase 2 beta Homo sapiens 114-152 9603937-4 1998 Engagement of T-cell or B-cell antigen receptors leads to rapid tyrosine phosphorylation of Pyk2-H. Tyrosine 64-72 protein tyrosine kinase 2 beta Homo sapiens 92-98 9603937-6 1998 In addition, we show that glutathione S-transferase fusion proteins containing the carboxyl termini of Pyk2 and Pyk2-H bind to a different set of tyrosine-phosphorylated proteins in thymus lysates. Tyrosine 146-154 protein tyrosine kinase 2 beta Homo sapiens 103-107 9603937-6 1998 In addition, we show that glutathione S-transferase fusion proteins containing the carboxyl termini of Pyk2 and Pyk2-H bind to a different set of tyrosine-phosphorylated proteins in thymus lysates. Tyrosine 146-154 protein tyrosine kinase 2 beta Homo sapiens 112-118 9573036-0 1998 The related adhesion focal tyrosine kinase (RAFTK) is tyrosine phosphorylated and participates in colony-stimulating factor-1/macrophage colony-stimulating factor signaling in monocyte-macrophages. Tyrosine 27-35 protein tyrosine kinase 2 beta Homo sapiens 44-49 9573036-4 1998 Colony-stimulating factor-1 (CSF-1)/macrophage colony-stimulating factor (M-CSF) stimulation of THP1 cells increased the tyrosine phosphorylation of RAFTK; similar increases in phosphorylation were also detected after lipopolysaccharide stimulation. Tyrosine 121-129 protein tyrosine kinase 2 beta Homo sapiens 149-154 9704064-9 1997 The activation of protein tyrosine kinase (PTK) is an important mechanism underlying transmembrane signaling and, moreover, protein tyrosine phosphorylations, stimulated by zymosan receptor-mediated activation, might be caused by the activation of specific PTK, whereas activation by PMA is probably mediated through another PKC type. Tyrosine 26-34 protein tyrosine kinase 2 beta Homo sapiens 43-46 9704064-9 1997 The activation of protein tyrosine kinase (PTK) is an important mechanism underlying transmembrane signaling and, moreover, protein tyrosine phosphorylations, stimulated by zymosan receptor-mediated activation, might be caused by the activation of specific PTK, whereas activation by PMA is probably mediated through another PKC type. Tyrosine 26-34 protein tyrosine kinase 2 beta Homo sapiens 257-260 9366396-4 1997 PYK-2 tyrosine phosphorylation was maximal at 1 min and started to decline 20 min after stimulation. Tyrosine 6-14 protein tyrosine kinase 2 beta Homo sapiens 0-5 9366396-5 1997 Engagement of alpha 4 beta 1 and alpha 5 beta 1 either with specific mAbs or after cell adhesion to fibronectin or its 120- and 40-kDa fragments also triggered PYK-2 tyrosine phosphorylation. Tyrosine 166-174 protein tyrosine kinase 2 beta Homo sapiens 160-165 9366396-6 1997 Stimulation of PYK-2 tyrosine phosphorylation was inhibited by the tyrosine kinase inhibitor herbimycin A, but not by EGTA, indicating that PYK-2 tyrosine phosphorylation is PTK, but not calcium, dependent. Tyrosine 21-29 protein tyrosine kinase 2 beta Homo sapiens 15-20 9366396-6 1997 Stimulation of PYK-2 tyrosine phosphorylation was inhibited by the tyrosine kinase inhibitor herbimycin A, but not by EGTA, indicating that PYK-2 tyrosine phosphorylation is PTK, but not calcium, dependent. Tyrosine 21-29 protein tyrosine kinase 2 beta Homo sapiens 140-145 9366396-6 1997 Stimulation of PYK-2 tyrosine phosphorylation was inhibited by the tyrosine kinase inhibitor herbimycin A, but not by EGTA, indicating that PYK-2 tyrosine phosphorylation is PTK, but not calcium, dependent. Tyrosine 67-75 protein tyrosine kinase 2 beta Homo sapiens 15-20 9366396-6 1997 Stimulation of PYK-2 tyrosine phosphorylation was inhibited by the tyrosine kinase inhibitor herbimycin A, but not by EGTA, indicating that PYK-2 tyrosine phosphorylation is PTK, but not calcium, dependent. Tyrosine 67-75 protein tyrosine kinase 2 beta Homo sapiens 140-145 9366396-7 1997 We also demonstrate that PYK-2 is constitutively associated with paxillin, which undergoes tyrosine phosphorylation with the same kinetics of PYK-2 upon beta 1 integrin ligation. Tyrosine 91-99 protein tyrosine kinase 2 beta Homo sapiens 25-30 9341192-6 1997 In addition, osmotic shock induced the tyrosine phosphorylation of several discrete proteins including Cbl, p130(cas), and the recently identified soluble tyrosine kinase, calcium-dependent tyrosine kinase (CADTK). Tyrosine 39-47 protein tyrosine kinase 2 beta Homo sapiens 172-205 9341192-6 1997 In addition, osmotic shock induced the tyrosine phosphorylation of several discrete proteins including Cbl, p130(cas), and the recently identified soluble tyrosine kinase, calcium-dependent tyrosine kinase (CADTK). Tyrosine 39-47 protein tyrosine kinase 2 beta Homo sapiens 207-212 9257837-0 1997 T cell receptor engagement induces tyrosine phosphorylation of FAK and Pyk2 and their association with Lck. Tyrosine 35-43 protein tyrosine kinase 2 beta Homo sapiens 71-75 9257837-6 1997 In this study we show that FAK and Pyk2 are two of the major 115-to-120-kDa proteins that become tyrosine phosphorylated in T cells following TCR complex stimulation. Tyrosine 97-105 protein tyrosine kinase 2 beta Homo sapiens 35-39 9257837-8 1997 The increase in tyrosine phosphorylation of both FAK and Pyk2, however, occurs in Lck-deficient cells suggesting that phosphorylation of both of these kinases does not require Lck. Tyrosine 16-24 protein tyrosine kinase 2 beta Homo sapiens 57-61 9242628-1 1997 The related adhesion focal tyrosine kinase (RAFTK) is tyrosine-phosphorylated following beta1 integrin or B cell antigen receptor stimulation in human B cells. Tyrosine 27-35 protein tyrosine kinase 2 beta Homo sapiens 44-49 9242628-2 1997 Two substrates that are tyrosine-phosphorylated following integrin ligation in B cells are p130(Cas) and the Cas family member human enhancer of filamentation 1 (HEF1), both of which can associate with RAFTK. Tyrosine 24-32 protein tyrosine kinase 2 beta Homo sapiens 202-207 9162070-4 1997 In contrast, while CADTK is not tyrosine-autophosphorylated in untreated cells, angiotensin II increases CADTK Tyr(P) by 5-10-fold. Tyrosine 111-114 protein tyrosine kinase 2 beta Homo sapiens 105-110 9162070-6 1997 In this report we assessed the contribution of CADTK and p125(FAK) to tyrosine phosphorylation of focal contact proteins. Tyrosine 70-78 protein tyrosine kinase 2 beta Homo sapiens 47-52 9162070-8 1997 A rapid increase in the tyrosine phosphorylation of each protein was detected after treatment with angiotensin II or other agonists that stimulate CADTK; the prolonged 3-4-fold increase in paxillin tyrosine phosphorylation was the most substantial change. Tyrosine 24-32 protein tyrosine kinase 2 beta Homo sapiens 147-152 9099734-2 1997 Stem cell factor, which potentiates the growth of megakaryocytes and their progenitors, and phorbol myristate acetate, which causes differentiation of megakaryocytic cell lines, induced the tyrosine phosphorylation of RAFTK but not of focal adhesion kinase. Tyrosine 190-198 protein tyrosine kinase 2 beta Homo sapiens 218-223 9099753-0 1997 Tyrosine phosphorylation of the novel protein-tyrosine kinase RAFTK during an early phase of platelet activation by an integrin glycoprotein IIb-IIIa-independent mechanism. Tyrosine 0-8 protein tyrosine kinase 2 beta Homo sapiens 62-67 9178638-2 1997 This study investigates whether protein tyrosine kinase (PTK) activity and tyrosine phosphorylation on p95(vav) and on the p85 regulatory subunit of phosphatidylinositol 3-kinase (PI3 kinase) play a role in the regulation of Ramos-BL B cell survival. Tyrosine 40-48 protein tyrosine kinase 2 beta Homo sapiens 57-60 9178638-3 1997 The PTK inhibitor herbimycin A (HA) triggers significant growth arrest prior to apoptosis from the G1-phase of the cell cycle, indicating that tyrosine phosphorylation of key proteins is critical for Ramos-BL cell cycle progression and survival. Tyrosine 143-151 protein tyrosine kinase 2 beta Homo sapiens 4-7 9099734-0 1997 Tyrosine phosphorylation of the related adhesion focal tyrosine kinase in megakaryocytes upon stem cell factor and phorbol myristate acetate stimulation and its association with paxillin. Tyrosine 0-8 protein tyrosine kinase 2 beta Homo sapiens 32-70 9099753-5 1997 Treatment of platelets with thrombin induced, in as early as 10 s, a rapid tyrosine phosphorylation of RAFTK in a time- and concentration-dependent manner. Tyrosine 75-83 protein tyrosine kinase 2 beta Homo sapiens 103-108 9099753-10 1997 Tyrosine phosphorylation of RAFTK in platelets is regulated by calcium and is mediated through the protein kinase C pathway. Tyrosine 0-8 protein tyrosine kinase 2 beta Homo sapiens 28-33 9099734-8 1997 Transient overexpression of a dominant-negative mutant of RAFTK inhibited significantly the tyrosine phosphorylation of paxillin upon phorbol myristate acetate stimulation. Tyrosine 92-100 protein tyrosine kinase 2 beta Homo sapiens 58-63 9099753-13 1997 These results demonstrate that RAFTK is tyrosine-phosphorylated during an early phase of platelet activation by an integrin- independent mechanism and is not dependent on platelet aggregation, suggesting different mechanisms of regulation for FAK and RAFTK phosphorylation during platelet activation. Tyrosine 40-48 protein tyrosine kinase 2 beta Homo sapiens 31-36 8995252-5 1997 Ligation of the B cell antigen receptor also induced tyrosine phosphorylation of RAFTK. Tyrosine 53-61 protein tyrosine kinase 2 beta Homo sapiens 81-86 9099753-13 1997 These results demonstrate that RAFTK is tyrosine-phosphorylated during an early phase of platelet activation by an integrin- independent mechanism and is not dependent on platelet aggregation, suggesting different mechanisms of regulation for FAK and RAFTK phosphorylation during platelet activation. Tyrosine 40-48 protein tyrosine kinase 2 beta Homo sapiens 251-256 8995252-4 1997 Here we report that RAFTK is a target for beta1-integrin-mediated tyrosine phosphorylation in both transformed and normal human B cells. Tyrosine 66-74 protein tyrosine kinase 2 beta Homo sapiens 20-25 9091579-7 1997 The tyrosine phosphorylation of RAFTK after T cell receptor-mediated stimulation was reduced by the pretreatment of cells with cytochalasin D, suggesting the role of the cytoskeleton in this process. Tyrosine 4-12 protein tyrosine kinase 2 beta Homo sapiens 32-37 9020138-6 1997 Both HEF1 and Cas were found to complex with the related adhesion focal tyrosine kinase (RAFTK), and when tyrosine phosphorylated, with the adapter molecule CrkL. Tyrosine 72-80 protein tyrosine kinase 2 beta Homo sapiens 89-94 7499242-13 1995 Treatment of the megakaryocytic CMK cells with thrombin caused a rapid induction of tyrosine phosphorylation of RAFTK protein. Tyrosine 84-92 protein tyrosine kinase 2 beta Homo sapiens 112-117 8939945-5 1996 Angiotensin II, which only slightly increases p125(FAK) tyrosine phosphorylation in GN4 cells, substantially increased CADTK tyrosine autophosphorylation and kinase activity. Tyrosine 125-133 protein tyrosine kinase 2 beta Homo sapiens 119-124 8695788-7 1996 It was phosphorylated on tyrosine in growing cultures of COS cells transfected with the pCDNAIII/flag-RAFTK expression vector containing the RAFTK cDNA ligated with the 8 amino acid flag peptide sequence. Tyrosine 25-33 protein tyrosine kinase 2 beta Homo sapiens 102-107 8695788-7 1996 It was phosphorylated on tyrosine in growing cultures of COS cells transfected with the pCDNAIII/flag-RAFTK expression vector containing the RAFTK cDNA ligated with the 8 amino acid flag peptide sequence. Tyrosine 25-33 protein tyrosine kinase 2 beta Homo sapiens 141-146 8695788-10 1996 Analysis of tyrosine-phosphorylated RAFTK from adherent transfected COS cells showed that the Src homology 2 (SH2) domains of the Src and Fyn protein kinases as well as the Grb2 adaptor protein were able to specifically associate with RAFTK. Tyrosine 12-20 protein tyrosine kinase 2 beta Homo sapiens 36-41 8695788-10 1996 Analysis of tyrosine-phosphorylated RAFTK from adherent transfected COS cells showed that the Src homology 2 (SH2) domains of the Src and Fyn protein kinases as well as the Grb2 adaptor protein were able to specifically associate with RAFTK. Tyrosine 12-20 protein tyrosine kinase 2 beta Homo sapiens 235-240 8695788-11 1996 Tyrosine phosphorylation of endogenous RAFTK was observed upon fibronectin-induced activation of human megakaryocytic cells. Tyrosine 0-8 protein tyrosine kinase 2 beta Homo sapiens 39-44 8819096-6 1996 This increased sensitivity of cells from senescent organisms to PTK inhibitors is most likely related to a lesser PTK activity since a significant decrease in the tyrosine phosphorylation of particular endogenous substrates was observed as a consequence of either CD3, CD4, CD8 or IL-2R activation. Tyrosine 163-171 protein tyrosine kinase 2 beta Homo sapiens 64-67 8666992-6 1996 Because later application of PTK inhibitors can reverse the seemingly stable insulin-mediated potentiation of NMDA current, it appears that tyrosine residues responsible for potentiation are continually rephosphorylated by some long-term PTK activity that was induced via insulin treatment. Tyrosine 140-148 protein tyrosine kinase 2 beta Homo sapiens 29-32 8666992-6 1996 Because later application of PTK inhibitors can reverse the seemingly stable insulin-mediated potentiation of NMDA current, it appears that tyrosine residues responsible for potentiation are continually rephosphorylated by some long-term PTK activity that was induced via insulin treatment. Tyrosine 140-148 protein tyrosine kinase 2 beta Homo sapiens 238-241 8778024-3 1996 Both anti-immunoglobulin M (anti-IgM) and H2O2 induced tyrosine phosphorylation through protein tyrosine kinase (PTK) activation. Tyrosine 55-63 protein tyrosine kinase 2 beta Homo sapiens 113-116 8778024-5 1996 Moreover, the tyrosine phosphorylation caused by the oxidant but not that induced by anti-IgM was markedly augmented by two inflammatory cytokines, tumour necrosis factor-alpha and interleukin-1 alpha, although these agents by themselves did not stimulate PTK activity nor induce tyrosine phosphorylation. Tyrosine 14-22 protein tyrosine kinase 2 beta Homo sapiens 256-259 7589123-4 1995 The implication of PTK in SEA-induced early B cell activation was then confirmed by demonstrating that cross-linked SEA induces a significant increase in the level of tyrosine phosphorylation in B cells. Tyrosine 167-175 protein tyrosine kinase 2 beta Homo sapiens 19-22 7589581-3 1995 Kinase activity of Src-PTK was essential for tyrosine phosphorylation of Syk and calcium mobilization upon receptor ligation, whereas these events were not affected by the mutation of SH2 domain. Tyrosine 45-53 protein tyrosine kinase 2 beta Homo sapiens 23-26 7535209-6 1995 As a result of the inhibited phosphatase activity, substrates for PTK become hyperphosphorylated on tyrosine residues, as shown by Western blot analysis of cell lysates from cultures of PBMC treated with vanadate. Tyrosine 100-108 protein tyrosine kinase 2 beta Homo sapiens 66-69 7544443-1 1995 The protein tyrosine kinase PYK2, which is highly expressed in the central nervous system, is rapidly phosphorylated on tyrosine residues in response to various stimuli that elevate the intracellular calcium concentration, as well as by protein kinase C activation. Tyrosine 12-20 protein tyrosine kinase 2 beta Homo sapiens 28-32 7840140-5 1995 Immunoblotting and immunoprecipitation experiments demonstrated that IFN-gamma induced tyrosine phosphorylation of the p91 component of ISGF3, which is blocked by preincubation of cells with PTK inhibitors. Tyrosine 87-95 protein tyrosine kinase 2 beta Homo sapiens 191-194 7528218-4 1994 PTK activation resulted in the tyrosine phosphorylation of multiple protein substrates and peaked at 0.5-1 min following CD19 cross-linking in all B-lineage cells examined. Tyrosine 31-39 protein tyrosine kinase 2 beta Homo sapiens 0-3 7513017-5 1994 The requirement of the Src-PTK to induce tyrosine phosphorylation and activation of Syk was also demonstrated by cotransfection of syk and src-PTK cDNAs into COS cells. Tyrosine 41-49 protein tyrosine kinase 2 beta Homo sapiens 27-30 7513017-5 1994 The requirement of the Src-PTK to induce tyrosine phosphorylation and activation of Syk was also demonstrated by cotransfection of syk and src-PTK cDNAs into COS cells. Tyrosine 41-49 protein tyrosine kinase 2 beta Homo sapiens 143-146 7513017-6 1994 These results suggest that the Src-PTK associated with BCR phosphorylates the tyrosine residue(s) of Syk upon receptor stimulation, enhancing the activity of Syk. Tyrosine 78-86 protein tyrosine kinase 2 beta Homo sapiens 35-38 8309282-3 1993 B cells from young subjects stimulated with the surface immunoglobulin (sIg) crosslinkers anti-IgM or Staphylococcus aureus Cowan I (SAC) demonstrated rapid increases in PTK mediated de novo tyrosine phosphorylation of endogenous proteins. Tyrosine 191-199 protein tyrosine kinase 2 beta Homo sapiens 170-173 7691610-3 1993 Cross-linking the surface CD40 of GC B cells with mAb consistently resulted in enhanced tyrosine phosphorylation on a number of distinct substrates: this process could be blocked, in a dose-dependent fashion, by pre-treating GC B cells with the selective protein tyrosine kinase(s) (PTK) inhibitor, herbimycin A. Tyrosine 88-96 protein tyrosine kinase 2 beta Homo sapiens 255-281 7691610-3 1993 Cross-linking the surface CD40 of GC B cells with mAb consistently resulted in enhanced tyrosine phosphorylation on a number of distinct substrates: this process could be blocked, in a dose-dependent fashion, by pre-treating GC B cells with the selective protein tyrosine kinase(s) (PTK) inhibitor, herbimycin A. Tyrosine 88-96 protein tyrosine kinase 2 beta Homo sapiens 283-286 1517559-5 1992 Ia-induced B cell aggregation and tyrosine phosphorylation were inhibited by genistein and by herbimycin A, inhibitors of tyrosine kinases (PTK). Tyrosine 34-42 protein tyrosine kinase 2 beta Homo sapiens 140-143 8484124-3 1993 Vav-associated GRF stimulation after TCR-CD3 ligation paralleled its tyrosine phosphorylation; both were blocked by a protein tyrosine kinase (PTK) inhibitor. Tyrosine 69-77 protein tyrosine kinase 2 beta Homo sapiens 143-146 1622386-2 1992 To this effect, samples were mixed with a PTK substrate, namely the polydispersed co-polymer of glutamic acid and tyrosine [poly(Glu, Tyr), M(r) from 30,000 to 94,000], and were simultaneously submitted to electrophoresis. Tyrosine 114-122 protein tyrosine kinase 2 beta Homo sapiens 42-45 1622386-2 1992 To this effect, samples were mixed with a PTK substrate, namely the polydispersed co-polymer of glutamic acid and tyrosine [poly(Glu, Tyr), M(r) from 30,000 to 94,000], and were simultaneously submitted to electrophoresis. Tyrosine 134-137 protein tyrosine kinase 2 beta Homo sapiens 42-45 1839225-3 1991 Recent studies suggest that the TCR-activated PTK regulates PLC activation by the phosphorylation of tyrosine residues of PLC gamma 1. Tyrosine 101-109 protein tyrosine kinase 2 beta Homo sapiens 46-49 2142837-3 1990 Both the interleukin-2 (IL2) growth factor receptor and the CD3/Ti T-cell antigen receptor contain a subunit which is phosphorylated on tyrosine by an activated protein kinase (PTK) during T-cell activation. Tyrosine 136-144 protein tyrosine kinase 2 beta Homo sapiens 161-175 2142837-3 1990 Both the interleukin-2 (IL2) growth factor receptor and the CD3/Ti T-cell antigen receptor contain a subunit which is phosphorylated on tyrosine by an activated protein kinase (PTK) during T-cell activation. Tyrosine 136-144 protein tyrosine kinase 2 beta Homo sapiens 177-180 34944965-10 2021 Moreover, PEAK3 activated PYK2, which promoted PEAK3 tyrosine phosphorylation, its association with GRB2 and ASAP1, and AKT signaling. Tyrosine 53-61 protein tyrosine kinase 2 beta Homo sapiens 26-30