PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 24850725-8 2014 Differences in tyrosine phosphorylation were associated with differences in function in that cross-linking of type III chimeras with TCR resulted in significantly greater IL-2 production. Tyrosine 15-23 interleukin 2 Homo sapiens 171-175 27936140-8 2016 Based on the inhibitory effect of WHI-P131, a selective drug of JAK3 kinase activity, we concluded that in quiescent primary T lymphocytes, the constitutive STAT3 and the IL-2-induced prolonged STAT5 activity (assayed by tyrosine phosphorylation) is mostly JAK3-independent. Tyrosine 221-229 interleukin 2 Homo sapiens 171-175 27153543-2 2016 IL-2 promotes the proliferation and effector function of T and NK cells through the tyrosine phosphorylation and activation of signal transducer and activator of transcription factors (STAT), especially STAT5. Tyrosine 84-92 interleukin 2 Homo sapiens 0-4 26425665-0 2015 SILAC-based quantification of changes in protein tyrosine phosphorylation induced by Interleukin-2 (IL-2) and IL-15 in T-lymphocytes. Tyrosine 49-57 interleukin 2 Homo sapiens 85-98 26425665-0 2015 SILAC-based quantification of changes in protein tyrosine phosphorylation induced by Interleukin-2 (IL-2) and IL-15 in T-lymphocytes. Tyrosine 49-57 interleukin 2 Homo sapiens 100-104 23341462-6 2013 Fsk treatment of MT-2 and Kit 225 T-cells inhibited IL-2-induced Stat5a/b tyrosine and serine phosphorylation, nuclear translocation, and DNA binding activity. Tyrosine 74-82 interleukin 2 Homo sapiens 52-56 25509109-4 2013 We observed phosphorylation of STAT5 no earlier than 5 h after PHA stimulation and the maximum phosphorylation was detected following 24 h. Exogenous IL-2 induced high level of STAT5 phosphorylation in the competent HBL as early as at 30 min and this level of STAT5 phosphorylation kept in the next 24-48 h. The correlation between alterations in tyrosine phosphorylation level of STAT5 and the expression of CD25 has been established. Tyrosine 347-355 interleukin 2 Homo sapiens 150-154 22402124-5 2012 IL-2 challenge enhanced tyrosine phosphorylation of Janus-activated kinase (JAK)1-3 and STAT5, and induced JAK1 and JAK2 to associate with STAT5 in IL-2-dependent ATL cells. Tyrosine 24-32 interleukin 2 Homo sapiens 0-4 22646506-6 2012 The differential phosphorylation profiles of protein tyrosine residues in these cells upon IL-2 stimulation were examined by two-dimensional gel electrophoresis. Tyrosine 53-61 interleukin 2 Homo sapiens 91-95 22402124-5 2012 IL-2 challenge enhanced tyrosine phosphorylation of Janus-activated kinase (JAK)1-3 and STAT5, and induced JAK1 and JAK2 to associate with STAT5 in IL-2-dependent ATL cells. Tyrosine 24-32 interleukin 2 Homo sapiens 148-152 22116305-5 2012 Activation by IL-2 led to tyrosine phosphorylation-dependent interactions between Jak3 and p52ShcA only at lower concentrations. Tyrosine 26-34 interleukin 2 Homo sapiens 14-18 21722762-4 2011 To gain insights into the downstream machinery activated by IL-2, we aimed to define the global tyrosine-phosphoproteome of IL-2 pathway in human T cell line Kit225 using high resolution mass spectrometry combined with phosphotyrosine immunoprecipitation and SILAC. Tyrosine 96-104 interleukin 2 Homo sapiens 124-128 21722762-2 2011 Association between IL-2 and its receptor (IL-2R), triggers activation of complex signaling cascade governed by tyrosine phosphorylation that culminates in transcription of genes involved in modulation of the immune response. Tyrosine 112-120 interleukin 2 Homo sapiens 20-24 21722762-4 2011 To gain insights into the downstream machinery activated by IL-2, we aimed to define the global tyrosine-phosphoproteome of IL-2 pathway in human T cell line Kit225 using high resolution mass spectrometry combined with phosphotyrosine immunoprecipitation and SILAC. Tyrosine 96-104 interleukin 2 Homo sapiens 60-64 21722762-5 2011 The molecular snapshot at 5min of IL-2 stimulation resulted in identification of 172 proteins among which 79 were found with increased abundance in the tyrosine-phosphorylated complexes, including several previously not reported IL-2 downstream effectors. Tyrosine 152-160 interleukin 2 Homo sapiens 34-38 21722762-5 2011 The molecular snapshot at 5min of IL-2 stimulation resulted in identification of 172 proteins among which 79 were found with increased abundance in the tyrosine-phosphorylated complexes, including several previously not reported IL-2 downstream effectors. Tyrosine 152-160 interleukin 2 Homo sapiens 229-233 18463202-8 2008 Furthermore, we demonstrated that the induction of interleukin-2 mRNA and protein in activated T cells was inhibited by morphine in cells, in which mu-opioid receptors had been induced by CD3/28 monoclonal antibodies (mAbs), and that this effect was blocked by the mu-opioid receptor-specific antagonist D-Phe-Cys-Tyr-D-Trp-Arg-Thr-Pen-Thr-NH(2). Tyrosine 314-317 interleukin 2 Homo sapiens 51-64 19923221-2 2010 Although the mechanisms governing IL-2-induced tyrosine phosphorylation and activation of JAK3/STAT5 have been extensively studied, the role of serine/threonine phosphorylation in controlling these effectors remains to be elucidated. Tyrosine 47-55 interleukin 2 Homo sapiens 34-38 19923221-3 2010 Using phosphoamino acid analysis, JAK3 and STAT5 were determined to be serine and tyrosine-phosphorylated in response to IL-2 stimulation of the human natural killer-like cell line, YT. Tyrosine 82-90 interleukin 2 Homo sapiens 121-125 19923221-7 2010 Moreover, inhibition of PP2A, but not PP1, diminished IL-2-induced tyrosine phosphorylation of IL-2Rbeta, JAK3, and STAT5, and abolished STAT5 DNA binding activity. Tyrosine 67-75 interleukin 2 Homo sapiens 54-58 17846080-2 2008 Cytokines such as growth hormone, prolactin, erythropoietin, and interleukin-2 stimulate the activation of STAT5a by tyrosine phosphorylation. Tyrosine 117-125 interleukin 2 Homo sapiens 65-78 16762027-7 2006 Investigating signal transduction in lymphoblasts induced by these cytokines, we found that not only IL-2 but also IL-4 enhances signal transducer and activator of transcription 3 (STAT3) tyrosine phosphorylation by IL-12. Tyrosine 188-196 interleukin 2 Homo sapiens 101-105 17640867-6 2007 We further show that LPXN suppressed the secretion of interleukin-2 by BCR-activated A20 B cells and that this inhibition was abrogated in the Y72F LPXN mutant, indicating that the phosphorylation of Tyr(72) is critical for the biological function of LPXN. Tyrosine 200-203 interleukin 2 Homo sapiens 54-67 17178161-5 2007 This perturbation of tyrosine phosphorylation was correlated with inhibition of activation-dependent signaling pathways regulating Erk-1/2 phosphorylation, NF-AT transcriptional activation and IL-2 production. Tyrosine 21-29 interleukin 2 Homo sapiens 193-197 17537734-0 2007 Janus kinase 3 regulates interleukin 2-induced mucosal wound repair through tyrosine phosphorylation of villin. Tyrosine 76-84 interleukin 2 Homo sapiens 25-38 17537734-6 2007 Activation by IL-2 led to rapid tyrosine phosphorylation and redistribution of Jak3. Tyrosine 32-40 interleukin 2 Homo sapiens 14-18 15170389-2 2004 Here, we report that, following IL-2 (interleukin-2) stimulation of human T lymphocytes, SHP-2 binds tyrosine residues 614 and 643 of human Gab2 through its N- and C-terminal SH2 domains respectively. Tyrosine 101-109 interleukin 2 Homo sapiens 32-36 16782030-4 2006 Importantly, HS1 tyrosine phosphorylation is required for its targeting to the IS and for its function in regulating actin dynamics and IL-2 promoter activity. Tyrosine 17-25 interleukin 2 Homo sapiens 136-140 16517754-3 2006 IL-2 with S28690 caused CLL cells to proliferate and increased their expression of B7-family members, production of TNF-alpha and IL-10, and levels of tyrosine-phosphorylated STAT-1 and STAT-3 proteins. Tyrosine 151-159 interleukin 2 Homo sapiens 0-4 15814281-4 2005 Moreover, exogenous IL-2 induced characteristic tyrosine phosphorylation events detectable by immunoblotting in these cells. Tyrosine 48-56 interleukin 2 Homo sapiens 20-24 15356145-1 2004 IL-2 stimulation of T lymphocytes induces the tyrosine phosphorylation and adaptor function of the insulin receptor substrate/Grb2-associated binder (Gab) family member, Gab2. Tyrosine 46-54 interleukin 2 Homo sapiens 0-4 15170389-4 2004 Expression of the Gab2 Tyr-614-->Phe (Y614F) mutant, defective in SHP-2 association, prevents ERK (extracellular-signal-regulated kinase) activation and expression of a luciferase reporter plasmid driven by the c-fos SRE (serum response element), indicating that interaction of SHP-2 with Gab2 is required for ERK activation in response to IL-2. Tyrosine 23-26 interleukin 2 Homo sapiens 343-347 15170389-2 2004 Here, we report that, following IL-2 (interleukin-2) stimulation of human T lymphocytes, SHP-2 binds tyrosine residues 614 and 643 of human Gab2 through its N- and C-terminal SH2 domains respectively. Tyrosine 101-109 interleukin 2 Homo sapiens 38-51 12101281-6 2002 Finally, raft disruption with methyl-beta-cyclodextrin (MBCD) attenuated IL-2-induced tyrosine phosphorylation events and selectively decreased the surface expression of the IL-2/15R beta-chain detected by flow cytometry. Tyrosine 86-94 interleukin 2 Homo sapiens 73-77 15302845-5 2004 The mutation of the first tyrosine residue in C-terminal ITAM did not show any impairment in induction of surface antigens and cell death, but rather increased IL-2 secretion and MAPK phosphorylation. Tyrosine 26-34 interleukin 2 Homo sapiens 160-164 15121872-8 2004 The homologous tyrosine in JAK3, tyrosine 785, is autophosphorylated in response to interleukin-2 stimulation and is required for SH2-B beta to bind JAK3. Tyrosine 15-23 interleukin 2 Homo sapiens 84-97 15121872-8 2004 The homologous tyrosine in JAK3, tyrosine 785, is autophosphorylated in response to interleukin-2 stimulation and is required for SH2-B beta to bind JAK3. Tyrosine 33-41 interleukin 2 Homo sapiens 84-97 12525482-4 2003 We have shown previously that a tyrosine-deficient IL-2Rbeta chain paired with wild type gammac stimulated enhancement of bcl-2 mRNA in IL-2-dependent T cells, but it was not determined which region of the IL-2R or which pathway was activated to direct this signaling response. Tyrosine 32-40 interleukin 2 Homo sapiens 51-55 12543077-5 2002 However, the constitutive tyrosine phosphorylation of Stat3 as well as IL-2 induced Stat5 tyrosine phosphorylation and DNA binding were unaffected by the stably transfected wild-type SHP2 as well as the inactive SHP2. Tyrosine 90-98 interleukin 2 Homo sapiens 71-75 11792427-10 2002 Thus, tyrosine phosphorylation of different adapter proteins and consequent interactions between signaling molecules described here may explain the molecular mechanisms of the additive effects of IL-2 stimulation and CD2 crosslinking on NK cell activation. Tyrosine 6-14 interleukin 2 Homo sapiens 196-200 11856346-7 2002 Depletion of membrane cholesterol using methyl-beta-cyclodextrin substantially reduced co-clustering of CD25 with CD48 and HLA-DR, as well as the IL-2 stimulated tyrosine-phosphorylation of STATs (signal transducer and activator of transcription). Tyrosine 162-170 interleukin 2 Homo sapiens 146-150 11781254-2 2002 Using human T cells, it was found that a recently discovered variant of the undecylprodigiosin family of antibiotics, PNU156804, previously shown to inhibit IL-2-induced cell proliferation, also blocks IL-2-mediated Jak3 auto-tyrosine phosphorylation, activation of Jak3 substrates signal transducers and activators of transcription (Stat) 5a and Stat5b, and extracellular regulated kinase 1 (Erk1) and Erk2 (p44/p42). Tyrosine 226-234 interleukin 2 Homo sapiens 157-161 11781254-2 2002 Using human T cells, it was found that a recently discovered variant of the undecylprodigiosin family of antibiotics, PNU156804, previously shown to inhibit IL-2-induced cell proliferation, also blocks IL-2-mediated Jak3 auto-tyrosine phosphorylation, activation of Jak3 substrates signal transducers and activators of transcription (Stat) 5a and Stat5b, and extracellular regulated kinase 1 (Erk1) and Erk2 (p44/p42). Tyrosine 226-234 interleukin 2 Homo sapiens 202-206 11535532-1 2001 Cytokines, such as interleukin-2 (IL-2), activate intracellular signaling pathways via rapid tyrosine phosphorylation of their receptors, resulting in the activation of many genes involved in cell growth and survival. Tyrosine 93-101 interleukin 2 Homo sapiens 19-32 11535532-1 2001 Cytokines, such as interleukin-2 (IL-2), activate intracellular signaling pathways via rapid tyrosine phosphorylation of their receptors, resulting in the activation of many genes involved in cell growth and survival. Tyrosine 93-101 interleukin 2 Homo sapiens 34-38 11513540-6 2001 Using different polyclonal antibodies that recognize either total STAT-4 protein or tyrosine-phosphorylated STAT-4, we can easily detect IL-12 and IFNalpha signaling in PHA/IL-2 blasts derived from peripheral blood lymphocytes. Tyrosine 84-92 interleukin 2 Homo sapiens 173-177 11468191-5 2001 Furthermore, IL-2 stimulation of PBMCs showed only minimal tyrosine phosphorylation of JAK-3. Tyrosine 59-67 interleukin 2 Homo sapiens 13-17 11260071-8 2001 Nine had no detectable gammac, four had abnormal gammac expression and no IL-2-induced JAK3 tyrosine phosphorylation, and one had normal gammac expression but no IL-2-induced JAK3 tyrosine phosphorylation, although JAK3 was present. Tyrosine 180-188 interleukin 2 Homo sapiens 162-166 11331873-5 2001 A tyrosine mutant of SOCS-3 still blocks STAT phosphorylation, but also strongly inhibits IL-2-dependent activation of ERK and cell proliferation. Tyrosine 2-10 interleukin 2 Homo sapiens 90-94 11238664-9 2001 Increased tyrosine phosphorylation was observed in FLS lysates following 30-min incubation with IL-2. Tyrosine 10-18 interleukin 2 Homo sapiens 96-100 11331873-2 2001 Here we show that SOCS-3 is strongly tyrosine-phosphorylated in response to many growth factors, including interleukin-2 (IL-2), erythropoietin (EPO), epidermal growth factor (EGF) and platelet-derived growth factor (PDGF). Tyrosine 37-45 interleukin 2 Homo sapiens 107-120 11331873-2 2001 Here we show that SOCS-3 is strongly tyrosine-phosphorylated in response to many growth factors, including interleukin-2 (IL-2), erythropoietin (EPO), epidermal growth factor (EGF) and platelet-derived growth factor (PDGF). Tyrosine 37-45 interleukin 2 Homo sapiens 122-126 11340297-0 2001 Gab2 is phosphorylated on tyrosine upon interleukin-2/interleukin-15 stimulation in mycosis-fungoides-derived tumor T cells and associates inducibly with SHP-2 and Stat5a. Tyrosine 26-34 interleukin 2 Homo sapiens 40-53 11145913-6 2001 Our study shows that Tip and StpC cooperate to upregulate IL-2 gene expression, that their effect is mediated primarily by NF-kappaB and NF-AT, which is partially dependent on tyrosine phosphorylation. Tyrosine 176-184 interleukin 2 Homo sapiens 58-62 11340297-5 2001 Preincubation of cells with the Src family kinase inhibitor, PP1, surprisingly increased the IL-2- and IL-15-induced tyrosine phosphorylation of Gab2, indicating that an Src family kinase member negatively regulates IL-2 receptor signaling in MF T cells. Tyrosine 117-125 interleukin 2 Homo sapiens 93-97 11340297-3 2001 We show that Gab2 was transiently phosphorylated by tyrosine in human mycosis fungoides (MF) tumor T cells upon IL-2 stimulation and that SHP2 as well as Stat5a associated inducibly with Gab2. Tyrosine 52-60 interleukin 2 Homo sapiens 112-116 11340297-4 2001 IL-15, but not IL-4, also induced tyrosine phosphorylation of Gab2, suggesting that the IL-2 receptor beta-chain is important for IL-2-induced Gab2 phosphorylation. Tyrosine 34-42 interleukin 2 Homo sapiens 88-92 11340297-5 2001 Preincubation of cells with the Src family kinase inhibitor, PP1, surprisingly increased the IL-2- and IL-15-induced tyrosine phosphorylation of Gab2, indicating that an Src family kinase member negatively regulates IL-2 receptor signaling in MF T cells. Tyrosine 117-125 interleukin 2 Homo sapiens 93-108 11097834-3 2000 By coprecipitation and far Western blotting analysis, we demonstrate that Crk (I/II) binds to tyrosine phosphorylated STAT5 in cells stimulated by cytokines such as thrombopoietin (TPO) and interleukin-2 (IL-2). Tyrosine 94-102 interleukin 2 Homo sapiens 190-203 11097834-3 2000 By coprecipitation and far Western blotting analysis, we demonstrate that Crk (I/II) binds to tyrosine phosphorylated STAT5 in cells stimulated by cytokines such as thrombopoietin (TPO) and interleukin-2 (IL-2). Tyrosine 94-102 interleukin 2 Homo sapiens 205-209 11034377-3 2000 We demonstrate that galectin-1 antagonizes TCR responses known to require costimulation and processive protein tyrosine phosphorylation, such as IL-2 production, but is permissive for TCR responses that only require partial TCR signals, such as IFN-gamma production, CD69 up-regulation, and apoptosis. Tyrosine 111-119 interleukin 2 Homo sapiens 145-149 11147695-4 2000 However, after IL-2 stimulation (200 U/ml, 20 min), immunoblots of cell lysates demonstrated attenuation of the IL-2-induced tyrosine phosphorylation of 65-75 kDa proteins by Daclizumab, but not by isotype controls. Tyrosine 125-133 interleukin 2 Homo sapiens 15-19 11147695-4 2000 However, after IL-2 stimulation (200 U/ml, 20 min), immunoblots of cell lysates demonstrated attenuation of the IL-2-induced tyrosine phosphorylation of 65-75 kDa proteins by Daclizumab, but not by isotype controls. Tyrosine 125-133 interleukin 2 Homo sapiens 112-116 11147695-5 2000 Since this is the molecular weight of the IL-2R beta- and gamma-chains, which are both tyrosine-phosphorylated by IL-2, we next examined the effect of Daclizumab on their IL-2-induced tyrosine phosphorylation. Tyrosine 87-95 interleukin 2 Homo sapiens 42-46 11147695-6 2000 In immunoblots of IL-2R beta- and gamma-chain-immunoprecipitates the tyrosine phosphorylation of both chains by IL-2, but not by IL-15, was attenuated in the presence of Daclizumab. Tyrosine 69-77 interleukin 2 Homo sapiens 18-22 11147695-7 2000 Furthermore, co-immunoprecipitation experiments showed that Daclizumab inhibited the IL-2-induced association of these chains, a prerequisite for their mutual tyrosine phosphorylation. Tyrosine 159-167 interleukin 2 Homo sapiens 85-89 10867021-0 2000 Protein-tyrosine kinase Pyk2 is involved in interleukin-2 production by Jurkat T cells via its tyrosine 402. Tyrosine 8-16 interleukin 2 Homo sapiens 44-57 10920190-3 2000 Dexamethasone inhibited IL-2-induced DNA binding, tyrosine phosphorylation, and nuclear translocation of Stat5 in primary T cells. Tyrosine 50-58 interleukin 2 Homo sapiens 24-28 10602027-1 2000 Upon IL-2 stimulation of T lymphocytes, the IL-2 receptor (IL-2R) becomes phosphorylated on specific tyrosine residues which serve as docking sites for proteins containing SH2 or phosphotyrosine binding domains. Tyrosine 101-109 interleukin 2 Homo sapiens 5-9 10617656-6 2000 Using CTLL-20 as a model system, we provide evidence that tyrosine dephosphorylation of STAT5 subsequent to IL-2-induced phosphorylation occurs in the absence of STAT5 nuclear translocation and new protein synthesis. Tyrosine 58-66 interleukin 2 Homo sapiens 108-112 10849438-0 2000 Tyrosine-phosphorylated Vav1 as a point of integration for T-cell receptor- and CD28-mediated activation of JNK, p38, and interleukin-2 transcription. Tyrosine 0-8 interleukin 2 Homo sapiens 122-135 10898517-1 2000 Immunoreceptor tyrosine-based activation motifs (ITAM), consisting of two YxxL segments, transmit signals leading to IL-2 gene activation in T cells. Tyrosine 15-23 interleukin 2 Homo sapiens 117-121 10779760-7 2000 Anti-phosphotyrosine immunoblotting demonstrated that IL-2 induced a rapid and time-dependent increase in tyrosine phosphorylation of several cellular proteins of molecular masses ranging from 35 to 180 kDa. Tyrosine 12-20 interleukin 2 Homo sapiens 54-58 10779760-8 2000 Interestingly, IL-2 caused a significant up-regulation of the constitutive levels of hck PTK mRNA and protein relative to medium-treated cells as well as an increase in p59hck tyrosine phosphorylation. Tyrosine 176-184 interleukin 2 Homo sapiens 15-19 10604984-3 2000 We find that signals for the inhibition of IL-2 secretion are delivered efficiently by a CTLA-4 mutant in which both cytoplasmic tyrosines have been replaced by phenylalanines. Tyrosine 129-138 interleukin 2 Homo sapiens 43-47 10400642-1 1999 T lymphocyte activation through stimulation of the T cell receptor complex and co-stimulatory receptors is associated with acute tyrosine phosphorylation of intracellular proteins, which in turn mediate downstream signaling events that regulate interleukin-2 expression and cell proliferation. Tyrosine 129-137 interleukin 2 Homo sapiens 245-258 10438903-3 1999 In contrast, IL-2 induces a rapid and transient tyrosine and serine phosphorylation of Stat3. Tyrosine 48-56 interleukin 2 Homo sapiens 13-17 10409671-0 1999 FYN-T-FYB-SLP-76 interactions define a T-cell receptor zeta/CD3-mediated tyrosine phosphorylation pathway that up-regulates interleukin 2 transcription in T-cells. Tyrosine 73-81 interleukin 2 Homo sapiens 124-137 10604992-6 2000 Specifically, inhibition of extracellular signal-regulated kinase (ERK) activation and of IL-2 production by CTLA-4-mediated signaling occurs in T cells expressing mutant CTLA-4 molecules lacking the cytoplasmic tyrosine residues, and in lck-deficient or ZAP-70-deficient T cells. Tyrosine 212-220 interleukin 2 Homo sapiens 90-94 10859484-3 2000 The analgesic domain is located around the 45th Tyr residue of human IL-2 in tertiary structure. Tyrosine 48-51 interleukin 2 Homo sapiens 69-73 12110904-3 2000 Therefore, in order to clarify the role of Jak3 in IL-2 signal transduction, the tyrosine-phosphorylation-involved yeast two-hybrid system was constructed and the N-terminal region JH3-JH7 of Jak3 was used as a bait to screen a peripheral blood cDNA library. Tyrosine 81-89 interleukin 2 Homo sapiens 51-55 10373416-7 1999 Overexpression of two dominant-negative types of CAST, a minimum CD3epsilon-binding domain and a tyrosine-mutant, strongly suppressed NFAT activation and interleukin-2 production. Tyrosine 97-105 interleukin 2 Homo sapiens 154-167 10373548-0 1999 SOCS-3 is tyrosine phosphorylated in response to interleukin-2 and suppresses STAT5 phosphorylation and lymphocyte proliferation. Tyrosine 10-18 interleukin 2 Homo sapiens 49-62 10373548-7 1999 Moreover, following IL-2 stimulation of T cells, SOCS-3 was able to interact with the IL-2 receptor complex, and in particular tyrosine phosphorylated Jak1 and IL-2Rbeta. Tyrosine 127-135 interleukin 2 Homo sapiens 20-24 10373548-8 1999 Additionally, in lymphocytes expressing SOCS-3 but not CIS, IL-2-induced tyrosine phosphorylation of STAT5b was markedly reduced, while there was only a weak effect on IL-3-mediated STAT5b tyrosine phosphorylation. Tyrosine 73-81 interleukin 2 Homo sapiens 60-64 10373548-8 1999 Additionally, in lymphocytes expressing SOCS-3 but not CIS, IL-2-induced tyrosine phosphorylation of STAT5b was markedly reduced, while there was only a weak effect on IL-3-mediated STAT5b tyrosine phosphorylation. Tyrosine 189-197 interleukin 2 Homo sapiens 60-64 9886399-5 1999 In this study, we show that in primary NK cells and NK cell lines, in addition to the activation of STAT1 and STAT5, IL-2 induces tyrosine phosphorylation of STAT4, a STAT previously reported to be activated only in response to IL-12 and IFN-alpha. Tyrosine 130-138 interleukin 2 Homo sapiens 117-121 10421845-7 1999 This membrane-proximal region can also mediate the IL-2-induced tyrosine phosphorylation of the p85 subunit of phosphatidylinositol-3-kinase (PI3K). Tyrosine 64-72 interleukin 2 Homo sapiens 51-55 10090941-8 1999 The alteration in IL-2 signaling included decreased tyrosine phosphorylation and DNA binding activity of STAT5 and poor induction of the c-Myc and c-Jun pathways. Tyrosine 52-60 interleukin 2 Homo sapiens 18-22 9973378-4 1999 IL-4 suppressed activation of DNA binding and tyrosine phosphorylation of the transcription factor Stat5 by IL-2, and suppressed the expression of the IL-2-inducible genes CD25, CIS, the PGE2 receptor, and cytokine responsive (CR) genes CR1 and CR8. Tyrosine 46-54 interleukin 2 Homo sapiens 108-112 10369681-3 1999 Indirect immunofluorescence shows that IL-2 controls the cellular distribution of Aiolos and induces its tyrosine phosphorylation, required for dissociation from Ras. Tyrosine 105-113 interleukin 2 Homo sapiens 39-43 10201984-7 1999 A specific inhibitor of p38 MAP kinase completely inhibits the serine phosphorylation of STAT1 and STAT3 induced by IL-12 and IL-2 and abrogates the functional synergy between IL-12 and IL-2 without affecting STAT tyrosine phosphorylation. Tyrosine 214-222 interleukin 2 Homo sapiens 126-130 10088663-0 1999 Defective IL2 gene expression in newborn is accompanied with impaired tyrosine-phosphorylation in T cells. Tyrosine 70-78 interleukin 2 Homo sapiens 10-13 10088663-7 1999 From these results, it is tempting to speculate that suboptimal capacities of cord T cells to up-regulate tyrosine phosphorylation might contribute to defective IL2 production in neonates. Tyrosine 106-114 interleukin 2 Homo sapiens 161-164 9915850-9 1999 Thus, ligation of CD28 with either CD80 or CD86 leads to shared early signal transduction events such as the tyrosine phosphorylation of CBL and VAV, to NFAT-mediated transcriptional activation, and to the costimulation of interleukin-2 and granulocyte-macrophage colony-stimulating factor production. Tyrosine 109-117 interleukin 2 Homo sapiens 223-289 9660791-0 1998 A new tyrosine-phosphorylated 97-kDa adaptor protein mediates interleukin-2-induced association of SHP-2 with p85-phosphatidylinositol 3-kinase in human T lymphocytes. Tyrosine 6-14 interleukin 2 Homo sapiens 62-75 9811525-4 1998 As determined by tyrosine phosphorylation, nuclear translocation and binding to an hSIE-oligonucleotide probe, IL-2 and IL-15 activated the slowly migrating isoform, Stat3alpha. Tyrosine 17-25 interleukin 2 Homo sapiens 111-115 9785040-3 1998 The analgesic domain of IL-2 may be composed of the 44th Phe, 45th Tyr, 107th Tyr, and 117th Phe residues that are located closely in the tertiary structure of IL-2. Tyrosine 67-70 interleukin 2 Homo sapiens 24-28 9785040-3 1998 The analgesic domain of IL-2 may be composed of the 44th Phe, 45th Tyr, 107th Tyr, and 117th Phe residues that are located closely in the tertiary structure of IL-2. Tyrosine 67-70 interleukin 2 Homo sapiens 160-164 9785040-3 1998 The analgesic domain of IL-2 may be composed of the 44th Phe, 45th Tyr, 107th Tyr, and 117th Phe residues that are located closely in the tertiary structure of IL-2. Tyrosine 78-81 interleukin 2 Homo sapiens 24-28 9785040-3 1998 The analgesic domain of IL-2 may be composed of the 44th Phe, 45th Tyr, 107th Tyr, and 117th Phe residues that are located closely in the tertiary structure of IL-2. Tyrosine 78-81 interleukin 2 Homo sapiens 160-164 9749527-6 1998 These results suggested that some of signaling chains containing ITAM may utilize a signal pathway without substrate tyrosine phosphorylation during T-cell activation leading to the IL-2 secretion. Tyrosine 117-125 interleukin 2 Homo sapiens 182-186 9407053-5 1997 Tyrosine phosphorylation of Hrs is induced rapidly after stimulation with IL-2 and granulocyte-macrophage colony-stimulating factor as well as hepatocyte growth factor. Tyrosine 0-8 interleukin 2 Homo sapiens 74-78 9613985-6 1998 Furthermore, both CT compounds inhibited constitutive tyrosine phosphorylation of two proteins: Jak3 and STAT5 which are key downstream elements in the signal transduction pathway activated by IL-2 and the other cytokines. Tyrosine 54-62 interleukin 2 Homo sapiens 193-197 9618521-3 1998 We show that IL-2 induces tyrosine phosphorylation of a 125-kDa protein and homotypic adhesion in beta2 integrin (CD18)-positive but not in beta2-integrin-negative T cells. Tyrosine 26-34 interleukin 2 Homo sapiens 13-17 9618521-4 1998 EDTA, an inhibitor of integrin adhesion, blocks IL-2-induced tyrosine phosphorylation of the 125-kDa protein but not other proteins in beta2-integrin-positive T cells. Tyrosine 61-69 interleukin 2 Homo sapiens 48-52 9618521-7 1998 Thus, IL-2 induces strong tyrosine phosphorylation of fakB in beta2-integrin-positive but not in beta2-integrin-negative T cells, and CD18 mAb selectively blocks IL-2-induced fakB-tyrosine phosphorylation in beta2-integrin-positive T cells. Tyrosine 26-34 interleukin 2 Homo sapiens 6-10 9618521-7 1998 Thus, IL-2 induces strong tyrosine phosphorylation of fakB in beta2-integrin-positive but not in beta2-integrin-negative T cells, and CD18 mAb selectively blocks IL-2-induced fakB-tyrosine phosphorylation in beta2-integrin-positive T cells. Tyrosine 180-188 interleukin 2 Homo sapiens 6-10 9618521-7 1998 Thus, IL-2 induces strong tyrosine phosphorylation of fakB in beta2-integrin-positive but not in beta2-integrin-negative T cells, and CD18 mAb selectively blocks IL-2-induced fakB-tyrosine phosphorylation in beta2-integrin-positive T cells. Tyrosine 180-188 interleukin 2 Homo sapiens 162-166 9520455-1 1998 Interleukin 2 (IL-2) rapidly induces tyrosine phosphorylation of intracellular substrates, including the IL-2 receptor beta chain (IL-2Rbeta), Janus kinase 1 (Jak1), Jak3, signal transducer/activator of transcription proteins, and Shc, but the mechanism underlying dephosphorylation of these proteins is not known. Tyrosine 37-45 interleukin 2 Homo sapiens 0-13 9520455-1 1998 Interleukin 2 (IL-2) rapidly induces tyrosine phosphorylation of intracellular substrates, including the IL-2 receptor beta chain (IL-2Rbeta), Janus kinase 1 (Jak1), Jak3, signal transducer/activator of transcription proteins, and Shc, but the mechanism underlying dephosphorylation of these proteins is not known. Tyrosine 37-45 interleukin 2 Homo sapiens 15-19 9520455-3 1998 We have found that IL-2 induces association of SHP-1 with the IL-2 receptor complex, and that once SHP-1 is recruited to the activated receptor it is able to decrease tyrosine phosphorylation of IL-2Rbeta and the associated tyrosine kinases Jak1 and Jak3. Tyrosine 167-175 interleukin 2 Homo sapiens 19-23 9494076-8 1998 In contrast, the distal three tyrosines in CD28 are required for optimal IL2 production as well as for optimal activation of the LCK and EMT/ITK tyrosine kinases. Tyrosine 30-39 interleukin 2 Homo sapiens 73-76 9494076-12 1998 Taken together the data indicate that the distal three tyrosines in CD28 are integral to the activation of LCK and EMT/ITK and for subsequent IL2 production. Tyrosine 55-64 interleukin 2 Homo sapiens 142-145 9461587-0 1998 Interleukin-2 stimulation induces tyrosine phosphorylation of p120-Cbl and CrkL and formation of multimolecular signaling complexes in T lymphocytes and natural killer cells. Tyrosine 34-42 interleukin 2 Homo sapiens 0-13 9461587-1 1998 Interleukin (IL)-2, a major growth and differentiation factor for T lymphocytes, was found to induce tyrosine phosphorylation of the proto-oncogene products p120-Cbl and CrkL in IL-2-dependent cell lines. Tyrosine 101-109 interleukin 2 Homo sapiens 0-18 9461587-1 1998 Interleukin (IL)-2, a major growth and differentiation factor for T lymphocytes, was found to induce tyrosine phosphorylation of the proto-oncogene products p120-Cbl and CrkL in IL-2-dependent cell lines. Tyrosine 101-109 interleukin 2 Homo sapiens 178-182 9461587-4 1998 In addition, we demonstrate that the SH2-SH3-SH3 adaptor protein CrkL is tyrosine-phosphorylated in an IL-2-dependent manner and, via its SH2 domain, associates with a large proportion of phosphorylated Cbl. Tyrosine 73-81 interleukin 2 Homo sapiens 103-107 9461587-6 1998 Furthermore, the association of CrkL and p85 is increased after IL-2 treatment by a mechanism involving intermediary tyrosine-phosphorylated proteins that remain to be identified. Tyrosine 117-125 interleukin 2 Homo sapiens 64-68 9382798-0 1997 Janus kinases in interleukin-2-mediated signaling: JAK1 and JAK3 are differentially regulated by tyrosine phosphorylation. Tyrosine 97-105 interleukin 2 Homo sapiens 17-30 9398404-5 1997 IL-2 and IL-7 were equivalent in their ability to induce tyrosine phosphorylation of STAT5A and STAT5B and to facilitate binding of these STATs to an immobilized GAS element. Tyrosine 57-65 interleukin 2 Homo sapiens 0-4 9357823-7 1997 Cytokines interleukin-1beta (IL-1beta), basic fibroblast growth factor, IL-2, epidermal growth factor, and transforming growth factor-beta modulated steady-state levels of Eck and B61 mRNA and regulated Eck activation as assessed by tyrosine phosphorylation. Tyrosine 233-241 interleukin 2 Homo sapiens 72-76 9200453-0 1997 TGF-beta inhibits IL-2-induced tyrosine phosphorylation and activation of Jak-1 and Stat 5 in T lymphocytes. Tyrosine 31-39 interleukin 2 Homo sapiens 18-22 9278318-0 1997 The Lck SH2 phosphotyrosine binding site is critical for efficient TCR-induced processive tyrosine phosphorylation of the zeta-chain and IL-2 production. Tyrosine 19-27 interleukin 2 Homo sapiens 137-141 9278318-7 1997 Here we demonstrate that mutation of amino acids important for SH2 phosphotyrosine binding significantly compromises the ability of F505 to enhance TCR-mediated protein tyrosine phosphorylation and Ag-induced IL-2 production in BI-141. Tyrosine 74-82 interleukin 2 Homo sapiens 209-213 9295038-2 1997 In contrast, mutations at the tyrosine or leucine residues in the C-terminal YxxL segment abrogate signals for interleukin (IL)-2 production but do not prevent tyrosine phosphorylation of the N-terminal tyrosine of the ITAM, lck association with the ITAM, activation of phospholipase C-gamma1 or calcium mobilization. Tyrosine 30-38 interleukin 2 Homo sapiens 111-129 9305919-2 1997 Interleukin 2 (IL-2) induces tyrosine phosphorylation of STATs 3 and 5 (signal transducer and activator of transcription). Tyrosine 29-37 interleukin 2 Homo sapiens 0-13 9305919-2 1997 Interleukin 2 (IL-2) induces tyrosine phosphorylation of STATs 3 and 5 (signal transducer and activator of transcription). Tyrosine 29-37 interleukin 2 Homo sapiens 15-19 9371209-7 1997 In contrast, alveolar macrophages inhibited the tyrosine phosphorylation of proteins which may be involved in IL-2 receptor-associated signal transduction. Tyrosine 48-56 interleukin 2 Homo sapiens 110-114 9200453-1 1997 Signaling through IL-2R, IL-2 induces tyrosine phosphorylation and activation of Jak-1 and Jak-3 kinases and Stat 3 and Stat 5 transcription factors leading to cell cycle progression of activated T cells from G1 to S phase. Tyrosine 38-46 interleukin 2 Homo sapiens 18-22 9200453-4 1997 We show here that treatment of activated T cells with TGF-beta inhibited IL-2-induced tyrosine phosphorylation and activation of Jak-1 and Stat 5 but not Jak-3 and Stat 3. Tyrosine 86-94 interleukin 2 Homo sapiens 73-77 8978277-4 1997 EPO, IL-2, and IL-15 activated the tyrosine phosphorylation of the adaptor protein, Shp2, and the association of Shp2/Grb2/cytokine receptor complexes. Tyrosine 35-43 interleukin 2 Homo sapiens 5-9 9182578-5 1997 We now report that IL2 stimulated marked phosphorylation of serine and tyrosine residues of both Stat5a and Stat5b in human T lymphocytes and in several IL2-responsive lymphocytic cell lines. Tyrosine 71-79 interleukin 2 Homo sapiens 19-22 9182578-5 1997 We now report that IL2 stimulated marked phosphorylation of serine and tyrosine residues of both Stat5a and Stat5b in human T lymphocytes and in several IL2-responsive lymphocytic cell lines. Tyrosine 71-79 interleukin 2 Homo sapiens 153-156 9162019-3 1997 We report here that IL-2-induced DNA-binding activity and tyrosine phosphorylation of STAT5 are stabilized by a proteasome inhibitor MG132; however, no detectable ubiquitination of the STAT proteins is observed. Tyrosine 58-66 interleukin 2 Homo sapiens 20-24 9162019-4 1997 This sustained STAT5 activation can be blocked by protein kinase inhibitors, which is consistent with the ability of the proteasome inhibitor to stabilize IL-2-induced tyrosine phosphorylation of Jak1 and Jak3. Tyrosine 168-176 interleukin 2 Homo sapiens 155-159 9129156-8 1997 Furthermore, the IL-2 stimulation also induced tyrosine phosphorylation of SHP-2 in the human IL-2-dependent T-cell line ILT-Mat. Tyrosine 47-55 interleukin 2 Homo sapiens 17-21 9129156-8 1997 Furthermore, the IL-2 stimulation also induced tyrosine phosphorylation of SHP-2 in the human IL-2-dependent T-cell line ILT-Mat. Tyrosine 47-55 interleukin 2 Homo sapiens 94-98 9115214-1 1997 Previous work has demonstrated that SLP-76, a Grb2-associated tyrosine-phosphorylated protein, augments Interleukin-2 promoter activity when overexpressed in the Jurkat T cell line. Tyrosine 62-70 interleukin 2 Homo sapiens 104-117 9129156-0 1997 Interleukin-2 induces tyrosine phosphorylation of SHP-2 through IL-2 receptor beta chain. Tyrosine 22-30 interleukin 2 Homo sapiens 0-13 9129156-1 1997 Coupling of interleukin-2 (IL-2) to the IL-2 receptor (IL-2R) induces rapid increase in tyrosine phosphorylation of cellular substrates through activation of non-receptor protein tyrosine kinases. Tyrosine 88-96 interleukin 2 Homo sapiens 12-25 9129156-1 1997 Coupling of interleukin-2 (IL-2) to the IL-2 receptor (IL-2R) induces rapid increase in tyrosine phosphorylation of cellular substrates through activation of non-receptor protein tyrosine kinases. Tyrosine 88-96 interleukin 2 Homo sapiens 27-31 9129156-4 1997 The IL-2-induced tyrosine phosphorylation of SHP-2 required the acidic region within the IL-2Rbeta chain where Src-family PTKs interact. Tyrosine 17-25 interleukin 2 Homo sapiens 4-8 9098929-1 1997 Stimulation of human CD4+ T cell lines with interleukin 2 (IL-2) induces tyrosine, serine and threonine phosphorylation of a series of proteins involved in the IL-2 receptor (IL-2R) signaling pathway. Tyrosine 73-81 interleukin 2 Homo sapiens 44-57 9098929-1 1997 Stimulation of human CD4+ T cell lines with interleukin 2 (IL-2) induces tyrosine, serine and threonine phosphorylation of a series of proteins involved in the IL-2 receptor (IL-2R) signaling pathway. Tyrosine 73-81 interleukin 2 Homo sapiens 59-63 8978277-3 1997 Although stimulation with IL-2 or IL-15 resulted in the rapid, dose-dependent tyrosine phosphorylation of Shc, stimulation with EPO failed to activate Shc. Tyrosine 78-86 interleukin 2 Homo sapiens 26-30 8946678-0 1996 Phosphorylation of each of the distal three tyrosines of the CD28 cytoplasmic tail is required for CD28-induced T cell IL-2 secretion. Tyrosine 44-53 interleukin 2 Homo sapiens 119-123 9326745-0 1997 A selective impairment of the IL-2 system in lymphocytes of patients with glioblastomas: increased level of soluble IL-2R and reduced protein tyrosine phosphorylation. Tyrosine 142-150 interleukin 2 Homo sapiens 30-34 9326745-5 1997 In addition, we found that competent T cells of glioblastoma patients exhibit lower levels of tyrosine phosphorylation in response to IL-2 as compared with cells of healthy donors. Tyrosine 94-102 interleukin 2 Homo sapiens 134-138 8870717-2 1996 On 5 min incubation of these cells with IL-2, we observed tyrosine phosphorylation of 105-kD and 110-kD proteins in NK cells and of 95-kD and 110-kD proteins in T cells. Tyrosine 58-66 interleukin 2 Homo sapiens 40-44 8870717-4 1996 With this phosphorylation, p52rhc was also tyrosine-phosphorylated and p21ras was activated by the short term (10 min) treatment of NK and T cells with IL-2. Tyrosine 43-51 interleukin 2 Homo sapiens 152-156 8870717-7 1996 In addition, the tyrosine phosphorylation of JAK3 by IL-2 was more prominent in NK cells than in T cells, but JAK1, JAK2, STAT1 alpha, STAT2 and STAT3 were not phosphorylated. Tyrosine 17-25 interleukin 2 Homo sapiens 53-57 8870717-8 1996 These results indicate that the IL-2 signal flows downstream via both ras-dependent and ras-independent pathways and that the superior killing activity of NK cells depends on their high susceptibility to protein tyrosine phosphorylation by IL-2. Tyrosine 212-220 interleukin 2 Homo sapiens 32-36 8870717-8 1996 These results indicate that the IL-2 signal flows downstream via both ras-dependent and ras-independent pathways and that the superior killing activity of NK cells depends on their high susceptibility to protein tyrosine phosphorylation by IL-2. Tyrosine 212-220 interleukin 2 Homo sapiens 240-244 8946678-4 1996 A receptor in which tyrosine 173 of the CD28 tail was mutated to phenylalanine was able to induce IL-2. Tyrosine 20-28 interleukin 2 Homo sapiens 98-102 8780729-1 1996 We molecularly cloned a cDNA coding for a novel phosphotyrosine molecule with a 70 kDa molecular mass, named STAM (signal transducing adaptor molecule), which is tyrosine-phosphorylated rapidly after stimulation with various cytokines such as IL-2, IL-3, IL-4, IL-7, GM-CSF, EGF and PDGF. Tyrosine 55-63 interleukin 2 Homo sapiens 243-247 8781427-6 1996 This mutation does not affect high-affinity IL-2 binding, but it partly decreases IL-2 endocytosis and prevents the downmodulation of the IL-2-receptor beta chain and the tyrosine phosphorylation of Jak 3 protein in response to IL-2. Tyrosine 171-179 interleukin 2 Homo sapiens 82-86 8781427-6 1996 This mutation does not affect high-affinity IL-2 binding, but it partly decreases IL-2 endocytosis and prevents the downmodulation of the IL-2-receptor beta chain and the tyrosine phosphorylation of Jak 3 protein in response to IL-2. Tyrosine 171-179 interleukin 2 Homo sapiens 82-86 8683106-2 1996 Gel mobility shift and immunoprecipitation analyses revealed that in addition to activating STAT3 (signal transducer and activator of transcription-3) and STAT5, IL-2 induced tyrosine and serine phosphorylation of STAT1 alpha, which formed IFN-gamma-activated sequence-binding complexes by itself and with STAT3. Tyrosine 175-183 interleukin 2 Homo sapiens 162-166 8764555-0 1996 Activation of p42erk2 MAPK and p90rsk by IL-2 occurs independently of protein kinase C. Stimulation of the human interleukin-2 (IL-2)-dependent cell line, Kit225, with IL-2 resulted in the rapid activation of microtubule-associated protein-2 kinase (MAPK), as demonstrated by the tyrosine phosphorylation of p42erk2, the reduced mobility of MAPK in SDS-PAGE gels, and the increased ability of lysates from these cells to phosphorylate myelin basic protein. Tyrosine 280-288 interleukin 2 Homo sapiens 41-45 8764555-0 1996 Activation of p42erk2 MAPK and p90rsk by IL-2 occurs independently of protein kinase C. Stimulation of the human interleukin-2 (IL-2)-dependent cell line, Kit225, with IL-2 resulted in the rapid activation of microtubule-associated protein-2 kinase (MAPK), as demonstrated by the tyrosine phosphorylation of p42erk2, the reduced mobility of MAPK in SDS-PAGE gels, and the increased ability of lysates from these cells to phosphorylate myelin basic protein. Tyrosine 280-288 interleukin 2 Homo sapiens 113-126 8764555-0 1996 Activation of p42erk2 MAPK and p90rsk by IL-2 occurs independently of protein kinase C. Stimulation of the human interleukin-2 (IL-2)-dependent cell line, Kit225, with IL-2 resulted in the rapid activation of microtubule-associated protein-2 kinase (MAPK), as demonstrated by the tyrosine phosphorylation of p42erk2, the reduced mobility of MAPK in SDS-PAGE gels, and the increased ability of lysates from these cells to phosphorylate myelin basic protein. Tyrosine 280-288 interleukin 2 Homo sapiens 128-132 8764555-0 1996 Activation of p42erk2 MAPK and p90rsk by IL-2 occurs independently of protein kinase C. Stimulation of the human interleukin-2 (IL-2)-dependent cell line, Kit225, with IL-2 resulted in the rapid activation of microtubule-associated protein-2 kinase (MAPK), as demonstrated by the tyrosine phosphorylation of p42erk2, the reduced mobility of MAPK in SDS-PAGE gels, and the increased ability of lysates from these cells to phosphorylate myelin basic protein. Tyrosine 280-288 interleukin 2 Homo sapiens 128-132 8683106-10 1996 Finally, IL-2 induced tyrosine phosphorylation of JAK1 and JAK3, while IL-12 induced phosphorylation of JAK2 and TYK2 in both preactivated primary NK and NK3.3 cells. Tyrosine 22-30 interleukin 2 Homo sapiens 9-13 8631883-6 1996 This IL-2-induced Stat5 activation was dependent on the presence of either of two tyrosines (Tyr-392 or Tyr-510) in the IL-2 receptor beta chain, indicating that either of these two tyrosines can serve as a docking site. Tyrosine 82-91 interleukin 2 Homo sapiens 5-9 8631883-6 1996 This IL-2-induced Stat5 activation was dependent on the presence of either of two tyrosines (Tyr-392 or Tyr-510) in the IL-2 receptor beta chain, indicating that either of these two tyrosines can serve as a docking site. Tyrosine 93-96 interleukin 2 Homo sapiens 5-9 8631883-6 1996 This IL-2-induced Stat5 activation was dependent on the presence of either of two tyrosines (Tyr-392 or Tyr-510) in the IL-2 receptor beta chain, indicating that either of these two tyrosines can serve as a docking site. Tyrosine 104-107 interleukin 2 Homo sapiens 5-9 8631883-6 1996 This IL-2-induced Stat5 activation was dependent on the presence of either of two tyrosines (Tyr-392 or Tyr-510) in the IL-2 receptor beta chain, indicating that either of these two tyrosines can serve as a docking site. Tyrosine 182-191 interleukin 2 Homo sapiens 5-9 8617237-3 1996 Our studies have shown that IL-2 rapidly induces both tyrosine phosphorylation and serine phosphorylation of STAT5 and that the two STAT5 tyrosine phosphoproteins detected in IL-2-activated cells differ in their levels of phosphorylation on serine residues. Tyrosine 54-62 interleukin 2 Homo sapiens 28-32 8648094-5 1996 Thus, a signal based upon Tyr 188 can deliver a costimulus for the enhancement of IL-2 production by Jurkat cells. Tyrosine 26-29 interleukin 2 Homo sapiens 82-86 8605323-7 1996 IL-2 stimulation of the transduced cell line resulted in appropriate tyrosine phosphorylation of both Jak1 and Jak3. Tyrosine 69-77 interleukin 2 Homo sapiens 0-4 8648094-3 1996 Mutation to Phe of all four cytoplasmic Tyr residues together (ALL F mutant) greatly reduced the ability of mCD28 to augment IL-2 production. Tyrosine 40-43 interleukin 2 Homo sapiens 125-129 8617237-3 1996 Our studies have shown that IL-2 rapidly induces both tyrosine phosphorylation and serine phosphorylation of STAT5 and that the two STAT5 tyrosine phosphoproteins detected in IL-2-activated cells differ in their levels of phosphorylation on serine residues. Tyrosine 54-62 interleukin 2 Homo sapiens 175-179 9592238-3 1996 It is inferred that the analgesic effect of IL-2 in CNS is mainly mediated through the analgesic domain which is composed of the 45th Tyr-residue, the 44th, 117th Phe-residues and other residues around the 45th-Tyr in tertiary structure, directly binding to the opioid receptors. Tyrosine 134-137 interleukin 2 Homo sapiens 44-48 9592238-3 1996 It is inferred that the analgesic effect of IL-2 in CNS is mainly mediated through the analgesic domain which is composed of the 45th Tyr-residue, the 44th, 117th Phe-residues and other residues around the 45th-Tyr in tertiary structure, directly binding to the opioid receptors. Tyrosine 211-214 interleukin 2 Homo sapiens 44-48 8630406-3 1996 Although the high-affinity IL-2R form (ie, the alpha+, beta+, gamma+ IL-2R form) was detectable in a small minority of primary AML samples as well as the KG-1 cell line and IL-2 binding to these cells was sufficient to initiate signal transduction as evidenced by an increase in overall protein tyrosine phosphorylation and more specifically in tyrosine phosphorylation of the Janus kinase (JAK) 3, in none of these cell types did exposure to IL-2 affect cell growth kinetics. Tyrosine 295-303 interleukin 2 Homo sapiens 27-31 8630406-3 1996 Although the high-affinity IL-2R form (ie, the alpha+, beta+, gamma+ IL-2R form) was detectable in a small minority of primary AML samples as well as the KG-1 cell line and IL-2 binding to these cells was sufficient to initiate signal transduction as evidenced by an increase in overall protein tyrosine phosphorylation and more specifically in tyrosine phosphorylation of the Janus kinase (JAK) 3, in none of these cell types did exposure to IL-2 affect cell growth kinetics. Tyrosine 345-353 interleukin 2 Homo sapiens 27-31 7479881-4 1995 Activation of STAT5 is measured both by IL-2-induced tyrosine phosphorylation and by IL-2-induced DNA binding. Tyrosine 53-61 interleukin 2 Homo sapiens 40-44 8696979-6 1996 Studies from the author"s laboratory have shown decreased Ca2+ mobilization and tyrosine phosphorylation correlated with suppressed T-cell IL-2 production and proliferation. Tyrosine 80-88 interleukin 2 Homo sapiens 139-143 8650263-6 1996 Data from the IL2, IL6 and IFN systems indicate a major role for the tyrosine phosphorylated receptor/JAK complexes (rather than substrate specificity of the JAKs per se) in STAT selection. Tyrosine 69-77 interleukin 2 Homo sapiens 14-17 8551221-3 1996 Both p46 and p52 forms of Shc were rapidly and transiently tyrosine phosphorylated upon CD16 or IL-2 stimulation with different kinetics. Tyrosine 59-67 interleukin 2 Homo sapiens 96-100 7479924-6 1995 The changes in the composition of IL-2Rs were accompanied by inhibition of IL-2-induced tyrosine phosphorylation of the Janus protein-tyrosine kinase 3 (Jak3) and signal transducers and activators of transcription called Stat3 and Stat5. Tyrosine 88-96 interleukin 2 Homo sapiens 34-38 7479924-6 1995 The changes in the composition of IL-2Rs were accompanied by inhibition of IL-2-induced tyrosine phosphorylation of the Janus protein-tyrosine kinase 3 (Jak3) and signal transducers and activators of transcription called Stat3 and Stat5. Tyrosine 88-96 interleukin 2 Homo sapiens 75-79 7665592-0 1995 Growth signal transduction by the human interleukin-2 receptor requires cytoplasmic tyrosines of the beta chain and non-tyrosine residues of the gamma c chain. Tyrosine 84-93 interleukin 2 Homo sapiens 40-53 8580378-4 1995 The present study verified the ability of IL-2 to cause tyrosine phosphorylation and activation of JAK1 and JAK3, but demonstrated that IL-2 stimulated JAK3 to a significantly larger extent than JAK1 in human T lymphocytes and the YT cell line. Tyrosine 56-64 interleukin 2 Homo sapiens 42-46 7568001-4 1995 IL-2 and IL-15 rapidly induced the tyrosine phosphorylation of STAT3 and STAT5, and DNA-binding complexes containing STAT3 and STAT5 were rapidly activated by these cytokines in T cells. Tyrosine 35-43 interleukin 2 Homo sapiens 0-4 7568001-6 1995 JAK1 and JAK3 were tyrosine-phosphorylated in response to IL-2 and IL-15. Tyrosine 19-27 interleukin 2 Homo sapiens 58-62 7665592-0 1995 Growth signal transduction by the human interleukin-2 receptor requires cytoplasmic tyrosines of the beta chain and non-tyrosine residues of the gamma c chain. Tyrosine 84-92 interleukin 2 Homo sapiens 40-53 7665592-1 1995 To evaluate the possible role for receptor-based tyrosine phosphorylation in growth signaling induced by interleukin-2 (IL-2), a series of substitution tyrosine mutants of the IL-2 receptor beta and gamma c chains was prepared and analyzed. Tyrosine 49-57 interleukin 2 Homo sapiens 105-118 7665592-1 1995 To evaluate the possible role for receptor-based tyrosine phosphorylation in growth signaling induced by interleukin-2 (IL-2), a series of substitution tyrosine mutants of the IL-2 receptor beta and gamma c chains was prepared and analyzed. Tyrosine 49-57 interleukin 2 Homo sapiens 120-124 7665592-2 1995 Concurrent mutation of all six of the cytoplasmic tyrosines present in the beta chain markedly inhibited IL-2-induced growth signaling in both pro-B and T cell lines. Tyrosine 50-59 interleukin 2 Homo sapiens 105-109 7665592-4 1995 Furthermore, at least one of the two cytoplasmic tyrosines that is required for beta chain function was found to serve as a phosphate acceptor site upon induction with IL-2. Tyrosine 49-58 interleukin 2 Homo sapiens 168-172 7760829-7 1995 In contrast, IL-2, IL-3, and erythropoietin induce the tyrosine phosphorylation of Stat5 while IL-12 uniquely induces the tyrosine phosphorylation of Stat4. Tyrosine 55-63 interleukin 2 Homo sapiens 13-17 7667294-9 1995 Receptors for IL-2, IL-4, IL-7, and IL-9 on intestinal epithelial cells lines appeared to be functional; stimulation with these cytokines caused rapid tyrosine phosphorylation of proteins. Tyrosine 151-159 interleukin 2 Homo sapiens 14-18 8948432-1 1995 We have previously shown that, during interleukin-2-driven G1/S transition in activated human T-lymphoblasts, a restricted group of cellular proteins become tyrosine phosphorylated de novo, and that p56lck is the major active tyrosine kinase at this stage of the cell cycle. Tyrosine 157-165 interleukin 2 Homo sapiens 38-51 7760829-7 1995 In contrast, IL-2, IL-3, and erythropoietin induce the tyrosine phosphorylation of Stat5 while IL-12 uniquely induces the tyrosine phosphorylation of Stat4. Tyrosine 122-130 interleukin 2 Homo sapiens 13-17 7852312-3 1995 In peripheral blood lymphocytes, we have observed an interleukin-2-dependent tyrosine phosphorylation of a 70-kDa protein and binding of tyrosine phosphorylated p70 to the SH2 domain of p56lck. Tyrosine 77-85 interleukin 2 Homo sapiens 53-66 7721895-1 1995 The tyrosine kinases JAK1 and JAK3 have been shown to undergo tyrosine phosphorylation in response to interleukin-2 (IL), IL4, IL7, and IL9, cytokines which share the common IL2 receptor gamma-chain (IL2R gamma), and evidence has been found for a preferential coupling of JAK3 to IL2R gamma and JAK1 to IL2R beta. Tyrosine 4-12 interleukin 2 Homo sapiens 102-115 7721895-1 1995 The tyrosine kinases JAK1 and JAK3 have been shown to undergo tyrosine phosphorylation in response to interleukin-2 (IL), IL4, IL7, and IL9, cytokines which share the common IL2 receptor gamma-chain (IL2R gamma), and evidence has been found for a preferential coupling of JAK3 to IL2R gamma and JAK1 to IL2R beta. Tyrosine 4-12 interleukin 2 Homo sapiens 117-119 7719938-3 1995 IL-7 and IL-15 induced the same complexes as IL-2, a feature explained by the existence of similar tyrosine-phosphorylated motifs in the cytoplasmic domains of IL-2R beta and IL-7R that can serve as docking sites for Stat proteins. Tyrosine 99-107 interleukin 2 Homo sapiens 45-49 7535338-5 1995 Moreover, JAK3 became tyrosine phosphorylated in response to IL-2, IL-4, and IL-7 but not response to IFN-gamma or granulocyte/macrophage colony-stimulating factor. Tyrosine 22-30 interleukin 2 Homo sapiens 61-65 7852312-3 1995 In peripheral blood lymphocytes, we have observed an interleukin-2-dependent tyrosine phosphorylation of a 70-kDa protein and binding of tyrosine phosphorylated p70 to the SH2 domain of p56lck. Tyrosine 137-145 interleukin 2 Homo sapiens 53-66 7528668-0 1994 Interleukin-2 induces tyrosine phosphorylation and nuclear translocation of stat3 in human T lymphocytes. Tyrosine 22-30 interleukin 2 Homo sapiens 0-13 7529282-7 1995 PBLs cocultured with an irradiated B cell line, Daudi, and IL-2 for 5 days attained a CD2-dominant cytokine-secretion pattern with identical tyrosine phosphorylation profiles as freshly isolated LPL or LPL T cell lines. Tyrosine 141-149 interleukin 2 Homo sapiens 59-63 7528775-0 1995 Interleukin 12 (IL-12) induces tyrosine phosphorylation of JAK2 and TYK2: differential use of Janus family tyrosine kinases by IL-2 and IL-12. Tyrosine 31-39 interleukin 2 Homo sapiens 127-131 7528775-4 1995 In this report we show that IL-12 and IL-2 induce tyrosine phosphorylation of distinct members of the Janus (JAK) family of protein tyrosine kinases in human T lymphocytes. Tyrosine 50-58 interleukin 2 Homo sapiens 38-42 7988557-2 1994 An IL-2-induced increase in JAK1 and JAK3, but not JAK2 or Tyk2, tyrosine phosphorylation was observed. Tyrosine 65-73 interleukin 2 Homo sapiens 3-7 7528668-5 1994 Apart from stat3, another cytoplasmic protein was tyrosine phosphorylated and subsequently translocated to the nucleus in response to IL-2. Tyrosine 50-58 interleukin 2 Homo sapiens 134-138 7988557-6 1994 However, IL-2 regulates the DNA binding and tyrosine phosphorylation of two STAT-like protein complexes which do not include STAT1, STAT2 or STAT3. Tyrosine 44-52 interleukin 2 Homo sapiens 9-13 7528668-8 1994 Taken together, we report that IL-2 induces tyrosine phosphorylation and subsequent nuclear translocation of stat3 and an as yet undefined 84-kDa protein in antigen-specific human T cell lines. Tyrosine 44-52 interleukin 2 Homo sapiens 31-35 7973657-3 1994 After culture with IL-2, IL-4, or IL-7, Jak3 kinase was tyrosine-phosphorylated, which correlated with the prevention of anergy. Tyrosine 56-64 interleukin 2 Homo sapiens 19-23 7750984-0 1994 NK-LAK induction with IL-2 is regulated by tyrosine phosphorylation of a 105-110 kDa protein. Tyrosine 43-51 interleukin 2 Homo sapiens 22-26 7750984-1 1994 Tyrosine phosphorylation of the cellular proteins of IL-2-stimulated NK cells was determined by anti-phosphotyrosine immunoblotting. Tyrosine 0-8 interleukin 2 Homo sapiens 53-57 7750984-2 1994 IL-2 induced tyrosine phosphorylation of a 105-110 kDa protein in a dose-dependent manner. Tyrosine 13-21 interleukin 2 Homo sapiens 0-4 7750984-3 1994 The tyrosine phosphorylation took place within 5 min after the addition of IL-2 to NK cells, and reached a maximal level in 15 min. Tyrosine 4-12 interleukin 2 Homo sapiens 75-79 7750984-4 1994 The degree of the tyrosine phosphorylation correlated with IL-2-induced LAK activity. Tyrosine 18-26 interleukin 2 Homo sapiens 59-63 7750984-10 1994 These results suggest that both tyrosine phosphorylation of the 105-110 kDa protein and translocation of [Ca++]i are essential for NK-LAK induction, and the tyrosine phosphorylation plays a critical role in the early stage of IL-2 signalling from the IL-2R beta chain. Tyrosine 32-40 interleukin 2 Homo sapiens 226-230 7750984-10 1994 These results suggest that both tyrosine phosphorylation of the 105-110 kDa protein and translocation of [Ca++]i are essential for NK-LAK induction, and the tyrosine phosphorylation plays a critical role in the early stage of IL-2 signalling from the IL-2R beta chain. Tyrosine 157-165 interleukin 2 Homo sapiens 226-230 7963551-10 1994 In summary, our data suggest that chronic low-level oxidative stress, via suppression of mitogen-induced transmembrane signaling (protein-tyrosine phosphorylation and calcium mobilization), causes a decrease in the DNA binding activity of transcription factors that regulate the IL-2 gene. Tyrosine 138-146 interleukin 2 Homo sapiens 279-283 7973658-4 1994 IL-2, IL-4, IL-7 (whose receptors are known to contain gamma c), and IL-9 (whose receptor is shown here to contain gamma c) induced the tyrosine phosphorylation and activation of the Janus family tyrosine kinases Jak1 and Jak3. Tyrosine 136-144 interleukin 2 Homo sapiens 0-4 7973659-2 1994 Stimulation with IL-2 induces the tyrosine phosphorylation and activation of the Janus kinases Jak1 and Jak3. Tyrosine 34-42 interleukin 2 Homo sapiens 17-21 7938054-2 1994 In order to clarify the effect of tyrosine phosphorylation of IL-2 receptors on cell-mediated cytotoxicity by natural killer (NK) cells, we studied the effect of a tyrosine kinase inhibitor, genistein, on the lethal effects of NK-rich cells for K562 cells. Tyrosine 34-42 interleukin 2 Homo sapiens 62-66 7938054-7 1994 The amount of phosphorylated tyrosine residues of 56-kDa protein, which was predominantly detected in NK-rich cells, was remarkably increased by IL-2 treatment. Tyrosine 29-37 interleukin 2 Homo sapiens 145-149 7938054-9 1994 These results suggested that IL-2 increased tyrosine phosphorylation and the affinity to IL-2R beta of the 56-kDa proteins in NK-rich cells. Tyrosine 44-52 interleukin 2 Homo sapiens 29-33 7938054-13 1994 The affinity of p56lck to IL-2R beta was considered to be regulated by IL-2-induced tyrosine phosphorylation. Tyrosine 84-92 interleukin 2 Homo sapiens 26-30 7935373-6 1994 In contrast, stimulation with IL-2 or the related cytokine IL-4 resulted in the rapid, dose-dependent tyrosine phosphorylation of JAK1 and an additional 116-kDa protein. Tyrosine 102-110 interleukin 2 Homo sapiens 30-34 7518451-8 1994 This putative JAK kinase underwent marked tyrosine phosphorylation in response to IL-2, IL-4, and IL-7, lymphoid growth factors that use the common IL-2 receptor gamma-chain, but not in response to prolactin. Tyrosine 42-50 interleukin 2 Homo sapiens 82-86 8088325-0 1994 Gene transfer of the interleukin (IL)-2 receptor beta chain into an IL-7-dependent pre-B cell line permits IL-2-driven proliferation: tyrosine phosphorylation of Shc is induced by IL-2 but not IL-7. Tyrosine 134-142 interleukin 2 Homo sapiens 107-111 8088325-0 1994 Gene transfer of the interleukin (IL)-2 receptor beta chain into an IL-7-dependent pre-B cell line permits IL-2-driven proliferation: tyrosine phosphorylation of Shc is induced by IL-2 but not IL-7. Tyrosine 134-142 interleukin 2 Homo sapiens 180-184 8088325-2 1994 Protein tyrosine phosphorylation was essential for IL-2 and IL-7-induced signal transduction since the tyrosine kinase inhibitor herbimycin A blocked proliferation in response to both factors. Tyrosine 8-16 interleukin 2 Homo sapiens 51-55 8088325-3 1994 Western blot analysis of tyrosine-phosphorylated proteins revealed that both IL-2 and IL-7 stimulation led to enhanced phosphorylation of proteins of 170-, 145-, 115- and 99-kDa, as well as induction of phosphorylation of a 96-kDa protein. Tyrosine 25-33 interleukin 2 Homo sapiens 77-81 8088325-7 1994 Taken together our results indicate that the IL-2- and IL-7-stimulated intracellular pathways are partially different and that Shc is a target of IL-2-, but not IL-7-, stimulated tyrosine phosphorylation. Tyrosine 179-187 interleukin 2 Homo sapiens 45-49 8088325-7 1994 Taken together our results indicate that the IL-2- and IL-7-stimulated intracellular pathways are partially different and that Shc is a target of IL-2-, but not IL-7-, stimulated tyrosine phosphorylation. Tyrosine 179-187 interleukin 2 Homo sapiens 146-150 8047842-3 1994 Our results demonstrate that tyrosine phosphorylation of pp100 stimulated by anti-CD3 is inhibited by cAMP both in the presence and absence of the phorbol ester PMA, and reflects the changes seen in IL2 mRNA expression and T-cell replication. Tyrosine 29-37 interleukin 2 Homo sapiens 199-202 7518451-8 1994 This putative JAK kinase underwent marked tyrosine phosphorylation in response to IL-2, IL-4, and IL-7, lymphoid growth factors that use the common IL-2 receptor gamma-chain, but not in response to prolactin. Tyrosine 42-50 interleukin 2 Homo sapiens 148-152 7910947-5 1994 Western blot analyses, and immunoprecipitations of cell lysates from 32P-preloaded NK cells demonstrated that seven major proteins are tyrosine phosphorylated in response to IL-2. Tyrosine 135-143 interleukin 2 Homo sapiens 174-178 7981618-1 1994 p56lck, a src family protein tyrosine kinase interacts with several T cell receptors, like: CD4, CD8, CD2 and the beta-chain of the IL2, thereby receptors devoid of kinase activity may transduce signals via tyr phosphorylation. Tyrosine 29-32 interleukin 2 Homo sapiens 132-135 8041779-3 1994 Tyrosine phosphorylation of JAK1 and JAK2 was induced upon IL-2 stimulation, and IL-2 activated the JAK2 kinase. Tyrosine 0-8 interleukin 2 Homo sapiens 59-63 8022485-2 1994 The subunits of the interleukin-2 receptor (IL-2R) lack intrinsic enzymatic activity, but protein tyrosine phosphorylation is a critical event following ligand binding and src family kinases, such as Lck, are known to be activated by IL-2 (refs 5-9). Tyrosine 98-106 interleukin 2 Homo sapiens 44-48 8022486-4 1994 Here we describe a new Jak family kinase, Jak-3, and demonstrate that Jak-3, and to a lesser extent Jak-1, are tyrosine phosphorylated and Jak-3 is activated in the responses to interleukin-2 and interleukin-4 in T cells and myeloid cells. Tyrosine 111-119 interleukin 2 Homo sapiens 178-191 8186198-6 1994 We found that tyrosine phosphorylation in response to IL-2 in HUT78 cells was strongly inhibited by a short preincubation with TGF-beta 1. Tyrosine 14-22 interleukin 2 Homo sapiens 54-58 7509794-1 1994 Interleukin-2 (IL-2) signaling results in tyrosine phosphorylation of the 75-kDa IL-2 receptor (IL-2R) beta chain and the activation of phosphatidylinositol 3"-kinase (PI3-K). Tyrosine 42-50 interleukin 2 Homo sapiens 0-13 7509794-1 1994 Interleukin-2 (IL-2) signaling results in tyrosine phosphorylation of the 75-kDa IL-2 receptor (IL-2R) beta chain and the activation of phosphatidylinositol 3"-kinase (PI3-K). Tyrosine 42-50 interleukin 2 Homo sapiens 15-19 7509794-3 1994 A fusion protein containing both the amino- and the carboxyl-terminal src homology 2 domains of p85 precipitates an 80-kDa tyrosine-phosphorylated protein (pp80) from the lysates of IL-2-stimulated, but not unstimulated, human T lymphoblasts. Tyrosine 123-131 interleukin 2 Homo sapiens 182-186 8307155-2 1994 Tyrosine phosphorylation has been implicated in interleukin 2 (IL-2)-induced growth signaling and the phosphorylation levels are regulated by the balance of tyrosine kinase and tyrosine phosphatase activities. Tyrosine 0-8 interleukin 2 Homo sapiens 48-61 8119884-0 1994 Interleukin-2-induced tyrosine phosphorylation of Shc proteins correlates with factor-dependent T cell proliferation. Tyrosine 22-30 interleukin 2 Homo sapiens 0-13 8119884-2 1994 Interaction of IL-2 with its receptor triggers tyrosine phosphorylation of a series of proteins and results in the activation of p21ras. Tyrosine 47-55 interleukin 2 Homo sapiens 15-19 8119884-3 1994 We report here that Shc, an SH2-containing adaptor protein, is tyrosine-phosphorylated following IL-2 stimulation. Tyrosine 63-71 interleukin 2 Homo sapiens 97-101 8119884-4 1994 IL-2-induced tyrosine phosphorylation of Shc was detectable within seconds following IL-2 addition, reaching its highest level by 15 min. Tyrosine 13-21 interleukin 2 Homo sapiens 0-4 8119884-4 1994 IL-2-induced tyrosine phosphorylation of Shc was detectable within seconds following IL-2 addition, reaching its highest level by 15 min. Tyrosine 13-21 interleukin 2 Homo sapiens 85-89 8119884-5 1994 Tyrosine phosphorylation of Shc was induced in multiple IL-2-dependent T cell lines and was found to correlate with IL-2-dependent cell proliferation. Tyrosine 0-8 interleukin 2 Homo sapiens 56-60 8119884-5 1994 Tyrosine phosphorylation of Shc was induced in multiple IL-2-dependent T cell lines and was found to correlate with IL-2-dependent cell proliferation. Tyrosine 0-8 interleukin 2 Homo sapiens 116-120 8119884-6 1994 Tyrosine-phosphorylated Shc was found to be capable of associating with the SH2 domain of Grb2 following IL-2 stimulation. Tyrosine 0-8 interleukin 2 Homo sapiens 105-109 8119884-7 1994 These results indicate that tyrosine phosphorylation of Shc and its association with Grb2 may be important events in IL-2-initiated signal transduction events in T cells. Tyrosine 28-36 interleukin 2 Homo sapiens 117-121 8307155-2 1994 Tyrosine phosphorylation has been implicated in interleukin 2 (IL-2)-induced growth signaling and the phosphorylation levels are regulated by the balance of tyrosine kinase and tyrosine phosphatase activities. Tyrosine 0-8 interleukin 2 Homo sapiens 63-67 8253732-1 1993 We report that in activated human T-lymphoblasts synchronized early in the G1-phase of the cell cycle, addition of interleukin 2 (IL-2) stimulates transition into S-phase in conjunction with specific and reproducible protein tyrosine phosphorylation events. Tyrosine 225-233 interleukin 2 Homo sapiens 115-128 8262983-8 1993 Csk, which phosphorylates tyrosine residues in the negative regulatory sites of Src family kinases, down-regulated Fyn- and Lck-mediated stimulation of the serum response element and Fyn-mediated enhancement of IL-2 promoter activity. Tyrosine 26-34 interleukin 2 Homo sapiens 211-215 8253732-1 1993 We report that in activated human T-lymphoblasts synchronized early in the G1-phase of the cell cycle, addition of interleukin 2 (IL-2) stimulates transition into S-phase in conjunction with specific and reproducible protein tyrosine phosphorylation events. Tyrosine 225-233 interleukin 2 Homo sapiens 130-134 7693677-0 1993 Characterization of an interleukin-2 (IL-2)-induced tyrosine phosphorylated 116-kDa protein associated with the IL-2 receptor beta-subunit. Tyrosine 52-60 interleukin 2 Homo sapiens 23-36 7693677-0 1993 Characterization of an interleukin-2 (IL-2)-induced tyrosine phosphorylated 116-kDa protein associated with the IL-2 receptor beta-subunit. Tyrosine 52-60 interleukin 2 Homo sapiens 38-42 7693677-0 1993 Characterization of an interleukin-2 (IL-2)-induced tyrosine phosphorylated 116-kDa protein associated with the IL-2 receptor beta-subunit. Tyrosine 52-60 interleukin 2 Homo sapiens 112-116 7693677-1 1993 In this paper we have extended previous results on interleukin-2 receptor (IL2-R) signal transduction and focused on the interleukin-2 (IL-2)-stimulated tyrosine phosphorylation of a 116-kDa protein (p116) observed in IL-2 responsive cells. Tyrosine 153-161 interleukin 2 Homo sapiens 136-140 7693677-1 1993 In this paper we have extended previous results on interleukin-2 receptor (IL2-R) signal transduction and focused on the interleukin-2 (IL-2)-stimulated tyrosine phosphorylation of a 116-kDa protein (p116) observed in IL-2 responsive cells. Tyrosine 153-161 interleukin 2 Homo sapiens 218-222 7693677-2 1993 This protein exhibited rapid and transient phosphorylation kinetics in both human T-lymphocytes and the YT cell line, attaining maximum tyrosine phosphorylation within 5 min of stimulation with IL-2. Tyrosine 136-144 interleukin 2 Homo sapiens 194-198 7693677-3 1993 Tyrosine phosphorylated p116 co-purified with activated IL-2 receptor beta-chain (IL2-R beta) when IL2-R complexes were covalently stabilized with the membrane-permeable cleavable cross-linking agent dithiobis(succimidyl propionate) prior to detergent cell lysis and immunoprecipitation with monoclonal anti-IL2-R beta antibodies. Tyrosine 0-8 interleukin 2 Homo sapiens 56-60 8409387-9 1993 Inhibition of T cell proliferation by Dex was associated with decreased IL-2-dependent tyrosine phosphorylation of several intracellular proteins and decreased phosphorylation of the retinoblastoma gene product Rb, a protein essential for controlling the progression of cells through the cell cycle. Tyrosine 87-95 interleukin 2 Homo sapiens 72-76 8260649-3 1993 In this review we summarize our findings to date regarding Raf-1, a serine/threonine-specific kinase that becomes phosphorylated on tyrosine residues and enzymatically activated in response to IL-2 stimulation. Tyrosine 132-140 interleukin 2 Homo sapiens 193-197 7903276-0 1993 Concurrent strong tyrosine phosphorylation of a 42,000 MW ERK and a 100,000 MW protein is associated with IL-2 production in human Jurkat T cells. Tyrosine 18-26 interleukin 2 Homo sapiens 106-110 7903276-1 1993 The tyrosine phosphorylated protein(s) responsible for the signalling for interleukin-2 (IL-2) production has not been clearly defined. Tyrosine 4-12 interleukin 2 Homo sapiens 74-87 7903276-1 1993 The tyrosine phosphorylated protein(s) responsible for the signalling for interleukin-2 (IL-2) production has not been clearly defined. Tyrosine 4-12 interleukin 2 Homo sapiens 89-93 7903276-2 1993 In this study, the relationship between IL-2 production and the protein tyrosine phosphorylation pattern of human Jurkat T cells was investigated using phosphotyrosine immunoblotting analysis. Tyrosine 72-80 interleukin 2 Homo sapiens 40-44 7903276-3 1993 With anti-CD3 or anti-CD2 activation the cells showed only a low (anti-CD3) or a moderate (anti-CD2) level of tyrosine phosphorylation of a 42,000 MW external signal-regulated kinase (ERK), which was accompanied by undetectable (anti-CD3) or low level (anti-CD2) IL-2 production. Tyrosine 110-118 interleukin 2 Homo sapiens 263-267 7903276-4 1993 In the presence of phorbol myristate acetate (PMA), large amounts of IL-2 were induced by both anti-CD3 and anti-CD2 stimulation, which was accompanied by strong concurrent tyrosine phosphorylation of the 42,000 MW ERK and a 100,000 MW protein. Tyrosine 173-181 interleukin 2 Homo sapiens 69-73 7903276-6 1993 These observations suggest that concurrent tyrosine phosphorylation of the 42,000 MW ERK and a 100,000 MW protein may be required for IL-2 production. Tyrosine 43-51 interleukin 2 Homo sapiens 134-138 8260651-6 1993 However, we have demonstrated that the serine rich (SD) region of the cytosolic domain of IL-2R beta is also required for association of a tyrosine kinase with the IL-2R complex and that IL-2 can induce proliferation and tyrosine phosphorylation in cell lines which lack the known SRC family kinases expressed by T lymphocytes. Tyrosine 139-147 interleukin 2 Homo sapiens 90-94 7679903-2 1993 The pattern of tyrosine phosphorylation induced by hIL-4 in transfectants expressing the chimeric receptor was very similar to that in transfectants expressing hIL-2R beta, but different from that in transfectants expressing hIL-4R, indicating that the chimeric receptor transduces the interleukin-2 like growth signal by hIL-4. Tyrosine 15-23 interleukin 2 Homo sapiens 286-299 7690544-0 1993 Interleukin-2 induces tyrosine phosphorylation of the vav proto-oncogene product in human T cells: lack of requirement for the tyrosine kinase lck. Tyrosine 22-30 interleukin 2 Homo sapiens 0-13 7690544-4 1993 We found rapid tyrosine phosphorylation of vav in response to IL-2 within 1 min, with maximum increase of phosphorylation of 5-fold occurring by 5 min after treatment in normal human T cells. Tyrosine 15-23 interleukin 2 Homo sapiens 62-66 7690544-5 1993 IL-2 stimulation of the human T-cell line YT and a subclone of the YT cell line (YTlck-) that does not express message for the src-family kinase p56lck also results in a rapid rate of tyrosine phosphorylation of vav of more than 5-fold by 5 min. Tyrosine 184-192 interleukin 2 Homo sapiens 0-4 7688728-0 1993 Interleukin-2-induced tyrosine phosphorylation of p52shc in T lymphocytes. Tyrosine 22-30 interleukin 2 Homo sapiens 0-13 7688728-1 1993 Stimulation of activated T cells with interleukin-2 (IL-2) results in the tyrosine phosphorylation of several intracellular proteins. Tyrosine 74-82 interleukin 2 Homo sapiens 38-51 7688728-1 1993 Stimulation of activated T cells with interleukin-2 (IL-2) results in the tyrosine phosphorylation of several intracellular proteins. Tyrosine 74-82 interleukin 2 Homo sapiens 53-57 7688728-2 1993 The present studies demonstrate that IL-2 stimulation induces phosphorylation of the src homology 2 domain-containing protein, p52shc, on both tyrosine and serine residues. Tyrosine 143-151 interleukin 2 Homo sapiens 37-41 7688728-4 1993 In addition, anti-Shc immunoprecipitates from IL-2-stimulated T cells contained a co-precipitating protein tyrosine kinase (PTK) activity that phosphorylated p52shc on tyrosine residues in immune complex kinase assays. Tyrosine 107-115 interleukin 2 Homo sapiens 46-50 8515060-6 1993 As expected, activation of IL-2R by IL-2 induced tyrosine phosphorylation of several proteins including p56lck, and class II cross-linking by mAb induced tyrosine phosphorylation of specific substrates including PLC-gamma 1. Tyrosine 49-57 interleukin 2 Homo sapiens 27-31 8515060-6 1993 As expected, activation of IL-2R by IL-2 induced tyrosine phosphorylation of several proteins including p56lck, and class II cross-linking by mAb induced tyrosine phosphorylation of specific substrates including PLC-gamma 1. Tyrosine 154-162 interleukin 2 Homo sapiens 27-31 8389729-8 1993 Recombinant IL-2 induced tyrosine phosphorylation of several intracellular proteins, including a 40,000 MW substrate which co-electrophoresed with ERK-2 on SDS-PAGE. Tyrosine 25-33 interleukin 2 Homo sapiens 12-16 8389729-12 1993 Together these data suggest that tyrosine phosphorylation is critical to IL-2-mediated signal transduction and that MAP kinase is one of the cellular intermediates involved in this pathway. Tyrosine 33-41 interleukin 2 Homo sapiens 73-77 8468460-8 1993 This inhibition was associated with the down-regulation of IL-2-mediated tyrosine phosphorylation of proteins of 120, 100, 85, 75, and 50 kDa. Tyrosine 73-81 interleukin 2 Homo sapiens 59-63 1472476-11 1992 Despite the absence of LCK or FYN, IL-2 induced similar patterns of rapid tyrosine phosphorylation. Tyrosine 74-82 interleukin 2 Homo sapiens 35-39 1385430-5 1992 Indeed, a 97-kDa phosphotyrosylprotein purified from IL-2-stimulated lymphoid cells as well as granulocyte-macrophage-CSF-stimulated myeloid cells bound to a polymer of glutamic acid and tyrosine which is a tyrosine kinase substrate. Tyrosine 187-195 interleukin 2 Homo sapiens 53-57 1402672-8 1992 Furthermore, tyrosine phosphorylation of the cellular proteins (p120, p98, p96, p54, and p38) was induced or enhanced in response to the addition of IL-2 in ED515-D and Kit225 cells, but not in the cell lines expressing pseudo-high affinity IL-2R. Tyrosine 13-21 interleukin 2 Homo sapiens 149-153 1385559-3 1992 Previous studies demonstrated that IL-2 stimulation induced the tyrosine phosphorylation of multiple proteins in NK cells, together with a prominent reduction in the electrophoretic mobility of p56lck. Tyrosine 64-72 interleukin 2 Homo sapiens 35-39 1384486-3 1992 In this transformant, we revealed that IL-2 induced erythroid differentiation and the same pattern of tyrosine phosphorylation as Epo. Tyrosine 102-110 interleukin 2 Homo sapiens 39-43 1385559-5 1992 Furthermore, in response to IL-2, p56lck itself undergoes complex alterations in serine and tyrosine phosphorylation. Tyrosine 92-100 interleukin 2 Homo sapiens 28-32 1385559-7 1992 In addition, IL-2 induced a marked increase in the phosphorylation of a COOH-terminal peptide containing the regulatory Tyr-505 residue of p56lck. Tyrosine 120-123 interleukin 2 Homo sapiens 13-17 1457964-1 1992 Interleukin 2 (IL-2)-induced tyrosine phosphorylation appears to play a major role in IL-2-induced cellular proliferation. Tyrosine 29-37 interleukin 2 Homo sapiens 0-13 1526279-0 1992 Cell type-specific tyrosine phosphorylation of IL-2 receptor beta chain in response to IL-2. Tyrosine 19-27 interleukin 2 Homo sapiens 47-51 1526279-2 1992 Lymphoid MOLT-4 and its transfectants expressing IL-2R beta either alone or with IL-2R alpha chain were found to be rapidly phosphorylated predominantly at tyrosine residues of IL-2R beta and to be affected in their growth in an IL-2-dependent manner. Tyrosine 156-164 interleukin 2 Homo sapiens 49-53 1457964-1 1992 Interleukin 2 (IL-2)-induced tyrosine phosphorylation appears to play a major role in IL-2-induced cellular proliferation. Tyrosine 29-37 interleukin 2 Homo sapiens 15-19 1457964-1 1992 Interleukin 2 (IL-2)-induced tyrosine phosphorylation appears to play a major role in IL-2-induced cellular proliferation. Tyrosine 29-37 interleukin 2 Homo sapiens 86-90 1751780-1 1991 Interleukin 2 (IL-2) has been shown to stimulate tyrosine phosphorylation of a number of proteins requiring only the p75 beta chain of the IL-2 receptor. Tyrosine 49-57 interleukin 2 Homo sapiens 0-13 1618313-0 1992 IL-2-dependent in vivo and in vitro tyrosine phosphorylation of IL-2 receptor gamma chain. Tyrosine 36-44 interleukin 2 Homo sapiens 0-4 1618313-0 1992 IL-2-dependent in vivo and in vitro tyrosine phosphorylation of IL-2 receptor gamma chain. Tyrosine 36-44 interleukin 2 Homo sapiens 64-68 1618313-2 1992 The present study demonstrated that the gamma chain, as well as the beta chain expressed on IL-2-responsive cells, is phosphorylated on tyrosine residues in an IL-2-dependent manner in vivo and in vitro. Tyrosine 136-144 interleukin 2 Homo sapiens 92-96 1618313-2 1992 The present study demonstrated that the gamma chain, as well as the beta chain expressed on IL-2-responsive cells, is phosphorylated on tyrosine residues in an IL-2-dependent manner in vivo and in vitro. Tyrosine 136-144 interleukin 2 Homo sapiens 160-164 1618313-3 1992 The in vivo tyrosine phosphorylation of both chains was similarly induced within 1 min after IL-2 stimulation, and their in vitro tyrosine phosphorylation with the anti-IL-2R beta antibody-directed immunocomplex was also increased by treatment of cells with IL-2. Tyrosine 12-20 interleukin 2 Homo sapiens 93-97 1618313-3 1992 The in vivo tyrosine phosphorylation of both chains was similarly induced within 1 min after IL-2 stimulation, and their in vitro tyrosine phosphorylation with the anti-IL-2R beta antibody-directed immunocomplex was also increased by treatment of cells with IL-2. Tyrosine 12-20 interleukin 2 Homo sapiens 169-173 1618313-3 1992 The in vivo tyrosine phosphorylation of both chains was similarly induced within 1 min after IL-2 stimulation, and their in vitro tyrosine phosphorylation with the anti-IL-2R beta antibody-directed immunocomplex was also increased by treatment of cells with IL-2. Tyrosine 130-138 interleukin 2 Homo sapiens 169-173 1760408-3 1991 Analysis of protein tyrosine phosphorylation showed that two common protein bands, pp95 and pp90, were phosphorylated by stimulation of either IL-2 or IL-3, suggesting the possible sharing of part of a signal transduction pathway between IL-2R and IL-3R. Tyrosine 20-28 interleukin 2 Homo sapiens 143-147 1760408-4 1991 Comparison of protein tyrosine phosphorylation profiles induced by IL-2 and IL-3 among a variety of cell lines revealed that the pp90 band is the common tyrosine phosphorylation substrate in the cell lines examined, although the general tyrosine phosphorylation pattern differed in each cell line. Tyrosine 22-30 interleukin 2 Homo sapiens 67-71 1315323-0 1992 Signal transduction by interleukin 2 in human T cells: activation of tyrosine and ribosomal S6 kinases and cell-cycle regulatory genes. Tyrosine 69-77 interleukin 2 Homo sapiens 23-36 1315323-4 1992 Within 5 min after addition of IL-2, rapid activation of tyrosine and ribosomal S6 kinases was detected. Tyrosine 57-65 interleukin 2 Homo sapiens 31-35 1315323-7 1992 Furthermore, the data indicate that tyrosine and ribosomal S6 kinases may be important for IL-2-induced cell growth. Tyrosine 36-44 interleukin 2 Homo sapiens 91-95 1602836-1 1992 Unlike many other growth factor receptors, the known subunits of the receptors for the Interleukins IL-2 and IL-3 lack intrinsic tyrosine kinase activity, and yet increases in the phosphorylation of proteins on tyrosines is a rapid event in hematolymphoid cells following stimulation with these lymphokines. Tyrosine 211-220 interleukin 2 Homo sapiens 100-104 1751780-1 1991 Interleukin 2 (IL-2) has been shown to stimulate tyrosine phosphorylation of a number of proteins requiring only the p75 beta chain of the IL-2 receptor. Tyrosine 49-57 interleukin 2 Homo sapiens 15-19 1751780-4 1991 IL-2 stimulated the in situ tyrosine phosphorylation of 97 kDa and 58 kDa proteins which bound to poly(Glu,Tyr)4:1, a substrate for tyrosine protein kinases, suggesting these proteins had characteristics found in almost all tyrosine kinases. Tyrosine 28-36 interleukin 2 Homo sapiens 0-4 1751780-8 1991 These proteins were phosphorylated on tyrosine residues by IL-2 stimulation of intact cells and ligand treatment of in vitro receptor extracts. Tyrosine 38-46 interleukin 2 Homo sapiens 59-63 2007186-1 1991 Interleukin-2 (IL-2) stimulates the rapid phosphorylation on tyrosine of several specific cellular proteins. Tyrosine 61-69 interleukin 2 Homo sapiens 0-13 1851793-10 1991 When IL-2-induced tyrosine phosphorylation events were examined, significant inhibition was seen of selected phosphoproteins in TGF-beta-pretreated cells. Tyrosine 18-26 interleukin 2 Homo sapiens 5-9 1706402-5 1991 The IL-2 portion of the molecule was shown to support the growth of the IL-2-dependent T cell line CTLL2, and the qualitative nature of the IL-2 signal was found to be the same as rIL-2 with respect to induction of tyrosine-phosphorylation of intracellular protein substrates. Tyrosine 215-223 interleukin 2 Homo sapiens 4-8 1911550-3 1991 However, binding of the lymphokine induces protein tyrosine phosphorylation and enhanced glucose transport in some myeloid progenitor cells. Tyrosine 51-59 interleukin 2 Homo sapiens 24-34 2007186-1 1991 Interleukin-2 (IL-2) stimulates the rapid phosphorylation on tyrosine of several specific cellular proteins. Tyrosine 61-69 interleukin 2 Homo sapiens 15-19 2007186-3 1991 In this study, we investigated the identities of the proteins phosphorylated on tyrosine in response to IL-2 stimulation to examine possible pathways of signal transduction. Tyrosine 80-88 interleukin 2 Homo sapiens 104-108 2007186-4 1991 By the use of immunoblotting with anti-phosphotyrosine antibodies, we demonstrate that IL-2 augments tyrosine phosphorylation of the IL-2 receptor beta chain in human cell lines expressing either high-affinity (alpha/beta) receptors or only the beta chain. Tyrosine 46-54 interleukin 2 Homo sapiens 87-91 2351654-10 1990 In contrast, the IL-2-induced tyrosine phosphorylation did not achieve maximal levels until 15 min after the addition of IL-2 and the proteins remained phosphorylated even after 60 min of incubation. Tyrosine 30-38 interleukin 2 Homo sapiens 17-21 1696599-0 1990 Protein tyrosine phosphorylation and p56lck modification in IL-2 or phorbol ester-activated human natural killer cells. Tyrosine 8-16 interleukin 2 Homo sapiens 60-64 1696599-4 1990 Immunoblot analyses with antiphosphotyrosine antibodies demonstrated that IL-2, a potent stimulus for NK cell proliferation and an agent that enhances NK cytotoxic function, induced the tyrosine phosphorylation of at least eight proteins in clonal CD16+/CD3-human NK cells. Tyrosine 36-44 interleukin 2 Homo sapiens 74-78 2351654-10 1990 In contrast, the IL-2-induced tyrosine phosphorylation did not achieve maximal levels until 15 min after the addition of IL-2 and the proteins remained phosphorylated even after 60 min of incubation. Tyrosine 30-38 interleukin 2 Homo sapiens 121-125 2351654-11 1990 In addition the tyrosine phosphorylations induced by OKT3 and IL-2 were not affected by prior stimulation with the other agent. Tyrosine 16-24 interleukin 2 Homo sapiens 62-66 2142837-3 1990 Both the interleukin-2 (IL2) growth factor receptor and the CD3/Ti T-cell antigen receptor contain a subunit which is phosphorylated on tyrosine by an activated protein kinase (PTK) during T-cell activation. Tyrosine 136-144 interleukin 2 Homo sapiens 9-22 1692253-6 1990 Similar concentrations of suramin were required to inhibit binding of 125I-labeled IL2, IL2-induced tyrosine phosphorylation, and IL2-induced proliferation, suggesting that these processes may be linked. Tyrosine 100-108 interleukin 2 Homo sapiens 88-91 1692253-6 1990 Similar concentrations of suramin were required to inhibit binding of 125I-labeled IL2, IL2-induced tyrosine phosphorylation, and IL2-induced proliferation, suggesting that these processes may be linked. Tyrosine 100-108 interleukin 2 Homo sapiens 88-91 2106566-0 1990 Interleukin 2 (IL-2)-induced tyrosine phosphorylation of IL-2 receptor p75. Tyrosine 29-37 interleukin 2 Homo sapiens 0-13 2106566-0 1990 Interleukin 2 (IL-2)-induced tyrosine phosphorylation of IL-2 receptor p75. Tyrosine 29-37 interleukin 2 Homo sapiens 15-19 2106566-2 1990 The present study using TU11 mAb demonstrates the IL-2-induced phosphorylation of IL-2Rp75 on tyrosine residues in IL-2-dependent T cells. Tyrosine 94-102 interleukin 2 Homo sapiens 50-54 2106566-2 1990 The present study using TU11 mAb demonstrates the IL-2-induced phosphorylation of IL-2Rp75 on tyrosine residues in IL-2-dependent T cells. Tyrosine 94-102 interleukin 2 Homo sapiens 82-86 2106566-3 1990 The tyrosine phosphorylation is mediated by the high affinity IL-2R, correlates with the IL-2-induced cell growth, and rapidly increases during the first 5 min of IL-2 stimulation. Tyrosine 4-12 interleukin 2 Homo sapiens 62-66 2106566-3 1990 The tyrosine phosphorylation is mediated by the high affinity IL-2R, correlates with the IL-2-induced cell growth, and rapidly increases during the first 5 min of IL-2 stimulation. Tyrosine 4-12 interleukin 2 Homo sapiens 89-93 2142837-3 1990 Both the interleukin-2 (IL2) growth factor receptor and the CD3/Ti T-cell antigen receptor contain a subunit which is phosphorylated on tyrosine by an activated protein kinase (PTK) during T-cell activation. Tyrosine 136-144 interleukin 2 Homo sapiens 24-27 2090880-6 1990 Using TU11 mAb, we secondly demonstrated that IL-2 rapidly induces tyrosine phosphorylation of both the beta and gamma chains in an IL-2-dose-dependent manner. Tyrosine 67-75 interleukin 2 Homo sapiens 46-50 2303462-0 1990 Interleukin 2-induced tyrosine phosphorylation. Tyrosine 22-30 interleukin 2 Homo sapiens 0-13 2303462-5 1990 As indicated by Western blotting with anti-phosphotyrosine-specific antibodies and confirmed by phosphoamino acid analysis, we now demonstrate that interaction of the T cell growth factor interleukin 2 (IL2) with its high affinity receptor on IL2-sensitive human peripheral blood lymphoblasts induces tyrosine phosphorylation of proteins of 92, 80, 78, 70-75, and 57 kDa. Tyrosine 50-58 interleukin 2 Homo sapiens 188-201 2303462-5 1990 As indicated by Western blotting with anti-phosphotyrosine-specific antibodies and confirmed by phosphoamino acid analysis, we now demonstrate that interaction of the T cell growth factor interleukin 2 (IL2) with its high affinity receptor on IL2-sensitive human peripheral blood lymphoblasts induces tyrosine phosphorylation of proteins of 92, 80, 78, 70-75, and 57 kDa. Tyrosine 50-58 interleukin 2 Homo sapiens 203-206 2303462-5 1990 As indicated by Western blotting with anti-phosphotyrosine-specific antibodies and confirmed by phosphoamino acid analysis, we now demonstrate that interaction of the T cell growth factor interleukin 2 (IL2) with its high affinity receptor on IL2-sensitive human peripheral blood lymphoblasts induces tyrosine phosphorylation of proteins of 92, 80, 78, 70-75, and 57 kDa. Tyrosine 50-58 interleukin 2 Homo sapiens 243-246 2303462-6 1990 IL2 induced tyrosine phosphorylation in YT 2C2 cells which express only the 75-kDa intermediate affinity IL2 binding molecule (IL2R beta) but not in cells which either express only the 55-kDa low affinity IL2 receptor molecule (IL2R alpha) or no IL2-binding sites. Tyrosine 12-20 interleukin 2 Homo sapiens 0-3 2303462-6 1990 IL2 induced tyrosine phosphorylation in YT 2C2 cells which express only the 75-kDa intermediate affinity IL2 binding molecule (IL2R beta) but not in cells which either express only the 55-kDa low affinity IL2 receptor molecule (IL2R alpha) or no IL2-binding sites. Tyrosine 12-20 interleukin 2 Homo sapiens 105-108 2303462-6 1990 IL2 induced tyrosine phosphorylation in YT 2C2 cells which express only the 75-kDa intermediate affinity IL2 binding molecule (IL2R beta) but not in cells which either express only the 55-kDa low affinity IL2 receptor molecule (IL2R alpha) or no IL2-binding sites. Tyrosine 12-20 interleukin 2 Homo sapiens 105-108 2303462-6 1990 IL2 induced tyrosine phosphorylation in YT 2C2 cells which express only the 75-kDa intermediate affinity IL2 binding molecule (IL2R beta) but not in cells which either express only the 55-kDa low affinity IL2 receptor molecule (IL2R alpha) or no IL2-binding sites. Tyrosine 12-20 interleukin 2 Homo sapiens 105-108 2090880-6 1990 Using TU11 mAb, we secondly demonstrated that IL-2 rapidly induces tyrosine phosphorylation of both the beta and gamma chains in an IL-2-dose-dependent manner. Tyrosine 67-75 interleukin 2 Homo sapiens 132-136 2090880-7 1990 The tyrosine phosphorylation of beta and gamma chains were also detected in the lymphoid transfectant cells but not in the fibroblastoid transfectant cells, indicating the correlation between tyrosine kinase activation and IL-2-mediated growth signaling. Tyrosine 4-12 interleukin 2 Homo sapiens 223-227 9354668-5 1997 Abnormalities in tyrosine phosphorylation of JAK3 in response to interleukin-2 (IL-2) and IL-4 were present in all patients. Tyrosine 17-25 interleukin 2 Homo sapiens 65-78 9354668-5 1997 Abnormalities in tyrosine phosphorylation of JAK3 in response to interleukin-2 (IL-2) and IL-4 were present in all patients. Tyrosine 17-25 interleukin 2 Homo sapiens 80-84 2511197-9 1989 These results demonstrate that the signaling capacity for the IL-2-induced tyrosine phosphorylation resides in the p75 receptor. Tyrosine 75-83 interleukin 2 Homo sapiens 62-66 35192696-4 2022 IL-2-induced tyrosine phosphorylation of Jak3 Y824 in human T cell line HuT78 cells was detected by using a phosphospecific, pY824, antibody. Tyrosine 13-21 interleukin 2 Homo sapiens 0-4 2511197-7 1989 IL-2-dependent tyrosine phosphorylation was observed in both cell lines and the concentrations of IL-2 needed to stimulate this phosphorylation were similar to that required for binding to the p75 receptor. Tyrosine 15-23 interleukin 2 Homo sapiens 0-4 2511197-7 1989 IL-2-dependent tyrosine phosphorylation was observed in both cell lines and the concentrations of IL-2 needed to stimulate this phosphorylation were similar to that required for binding to the p75 receptor. Tyrosine 15-23 interleukin 2 Homo sapiens 98-102 2583191-0 1989 Interleukin 2 stimulates tyrosine phosphorylation in T cell membrane fractions. Tyrosine 25-33 interleukin 2 Homo sapiens 0-13 2583191-3 1989 In an attempt to elucidate the biochemical pathway of signal transduction, we investigated the capacity of interleukin 2 to modulate tyrosine phosphorylation in T cell membranes. Tyrosine 133-141 interleukin 2 Homo sapiens 107-120 2583191-5 1989 Using as substrate a synthetic polymer of glutamic acid and tyrosine, we demonstrated a 3-4-fold stimulation of tyrosine phosphorylation in the presence of interleukin 2; this stimulating effect appeared to be well correlated with interleukin 2 function since (a) it was not observed in insensitive cells, (b) it required the presence of the high-affinity form of the receptor and (c) it was dose-dependent. Tyrosine 60-68 interleukin 2 Homo sapiens 156-169 2583191-5 1989 Using as substrate a synthetic polymer of glutamic acid and tyrosine, we demonstrated a 3-4-fold stimulation of tyrosine phosphorylation in the presence of interleukin 2; this stimulating effect appeared to be well correlated with interleukin 2 function since (a) it was not observed in insensitive cells, (b) it required the presence of the high-affinity form of the receptor and (c) it was dose-dependent. Tyrosine 60-68 interleukin 2 Homo sapiens 231-244 2583191-5 1989 Using as substrate a synthetic polymer of glutamic acid and tyrosine, we demonstrated a 3-4-fold stimulation of tyrosine phosphorylation in the presence of interleukin 2; this stimulating effect appeared to be well correlated with interleukin 2 function since (a) it was not observed in insensitive cells, (b) it required the presence of the high-affinity form of the receptor and (c) it was dose-dependent. Tyrosine 112-120 interleukin 2 Homo sapiens 156-169 2583191-5 1989 Using as substrate a synthetic polymer of glutamic acid and tyrosine, we demonstrated a 3-4-fold stimulation of tyrosine phosphorylation in the presence of interleukin 2; this stimulating effect appeared to be well correlated with interleukin 2 function since (a) it was not observed in insensitive cells, (b) it required the presence of the high-affinity form of the receptor and (c) it was dose-dependent. Tyrosine 112-120 interleukin 2 Homo sapiens 231-244 2583191-7 1989 At least two proteins of 115 and 58 kDa were consistently hyperphosphorylated on tyrosine in an interleukin-2-dependent manner. Tyrosine 81-89 interleukin 2 Homo sapiens 96-109 2583191-9 1989 Thus, we propose that interleukin 2 enhances tyrosine phosphorylation by stimulating a tyrosine kinase activity. Tyrosine 45-53 interleukin 2 Homo sapiens 22-35 2787319-3 1989 Here, using anti-phosphotyrosine antibodies to purify phosphotyrosyl proteins and two-dimensional gel analysis, we show that IL 2 stimulates rapid tyrosine phosphorylation of a variety of cellular proteins, including pp180, pp92, and pp42 in activated human T lymphocytes. Tyrosine 24-32 interleukin 2 Homo sapiens 125-129 2789316-0 1989 Comparative analysis of IL-2 and IL-3 induced tyrosine phosphorylation. Tyrosine 46-54 interleukin 2 Homo sapiens 24-28 2789316-2 1989 We have examined the involvement of tyrosine phosphorylation in IL2 and IL3 mediated signal transduction. Tyrosine 36-44 interleukin 2 Homo sapiens 64-67 2789316-7 1989 These results suggest that signal transduction pathways for IL2 and IL3 involve tyrosine phosphorylation of protein substrates common to both lymphoid and myeloid linages. Tyrosine 80-88 interleukin 2 Homo sapiens 60-63 2787350-5 1989 In fact, the tyrosine phosphorylated proteins did not coprecipitate with alpha Tac antibody and similar phosphoproteins were stimulated by IL-2 in IL-2R alpha- human large granular lymphocytes which express only the 70 to 75 kDa IL-2R beta subunit of the high affinity IL-2R. Tyrosine 13-21 interleukin 2 Homo sapiens 139-143 2787350-6 1989 Anti-Tac mAb could inhibit IL-2-stimulated tyrosine phosphorylation in activated T cells, which express both IL-2R subunits that together form the high affinity receptor complex, but not in large granular lymphocytes expressing only the IL-2R beta subunit. Tyrosine 43-51 interleukin 2 Homo sapiens 27-31 2787319-4 1989 In addition, we have examined IL 2-induced tyrosine phosphorylation in the human cell line YT2C2 which expresses mostly the beta subunit of the IL 2 receptor and the gibbon cell line MLA-144 which expresses only the beta subunit. Tyrosine 43-51 interleukin 2 Homo sapiens 30-34 2787319-5 1989 In both of these cell lines, IL 2 induced tyrosine phosphorylation of the same proteins phosphorylated in normal human T lymphocytes in response to IL 2. Tyrosine 42-50 interleukin 2 Homo sapiens 29-33 2787319-5 1989 In both of these cell lines, IL 2 induced tyrosine phosphorylation of the same proteins phosphorylated in normal human T lymphocytes in response to IL 2. Tyrosine 42-50 interleukin 2 Homo sapiens 148-152 31085588-4 2019 IL-2-induced tyrosine phosphorylation of STAT5 (pSTAT5) was proportional to CD25 levels on human CD4+ T cells and YT human NK cell line, directly demonstrating that CD25 promotes IL-2R signaling. Tyrosine 13-21 interleukin 2 Homo sapiens 0-4 32469452-5 2020 In addition, PTPN22 dephosphorylates EB1 at tyrosine-247 (Y247), which decreases the expression of the T-cell activation markers CD25 and CD69 and the phosphorylation levels of the TCR molecules ZAP-70, LAT, and Erk, leading to the eventual downregulation of the transcription factor NFAT and reduced the levels of secreted IL-2. Tyrosine 44-52 interleukin 2 Homo sapiens 324-328 32072341-8 2020 This led to impaired STAT tyrosine phosphorylation in response to IL-2 and IL-21, reduced expression of IL-2 target genes in patient CD4+ T cells, and reduced cell proliferation in response to IL-2 stimulation. Tyrosine 26-34 interleukin 2 Homo sapiens 66-70 32072341-8 2020 This led to impaired STAT tyrosine phosphorylation in response to IL-2 and IL-21, reduced expression of IL-2 target genes in patient CD4+ T cells, and reduced cell proliferation in response to IL-2 stimulation. Tyrosine 26-34 interleukin 2 Homo sapiens 75-79 32072341-8 2020 This led to impaired STAT tyrosine phosphorylation in response to IL-2 and IL-21, reduced expression of IL-2 target genes in patient CD4+ T cells, and reduced cell proliferation in response to IL-2 stimulation. Tyrosine 26-34 interleukin 2 Homo sapiens 75-79 31935623-2 2020 High-dose interleukin-2 (HD IL-2), which led to durable complete responses in a small fraction of patients by activating the interleukin-2 (IL-2) pathway, faded in popularity with the advent of oral tyrosine kinase inhibitors directed against the VEGF pathway (VEGFR-TKI) showing better tolerability, wider applicability, higher objective response rates, and longer progression-free survival than HD IL-2. Tyrosine 199-207 interleukin 2 Homo sapiens 10-23 31935623-2 2020 High-dose interleukin-2 (HD IL-2), which led to durable complete responses in a small fraction of patients by activating the interleukin-2 (IL-2) pathway, faded in popularity with the advent of oral tyrosine kinase inhibitors directed against the VEGF pathway (VEGFR-TKI) showing better tolerability, wider applicability, higher objective response rates, and longer progression-free survival than HD IL-2. Tyrosine 199-207 interleukin 2 Homo sapiens 28-32 31935623-2 2020 High-dose interleukin-2 (HD IL-2), which led to durable complete responses in a small fraction of patients by activating the interleukin-2 (IL-2) pathway, faded in popularity with the advent of oral tyrosine kinase inhibitors directed against the VEGF pathway (VEGFR-TKI) showing better tolerability, wider applicability, higher objective response rates, and longer progression-free survival than HD IL-2. Tyrosine 199-207 interleukin 2 Homo sapiens 125-138 31935623-2 2020 High-dose interleukin-2 (HD IL-2), which led to durable complete responses in a small fraction of patients by activating the interleukin-2 (IL-2) pathway, faded in popularity with the advent of oral tyrosine kinase inhibitors directed against the VEGF pathway (VEGFR-TKI) showing better tolerability, wider applicability, higher objective response rates, and longer progression-free survival than HD IL-2. Tyrosine 199-207 interleukin 2 Homo sapiens 140-144 31935623-2 2020 High-dose interleukin-2 (HD IL-2), which led to durable complete responses in a small fraction of patients by activating the interleukin-2 (IL-2) pathway, faded in popularity with the advent of oral tyrosine kinase inhibitors directed against the VEGF pathway (VEGFR-TKI) showing better tolerability, wider applicability, higher objective response rates, and longer progression-free survival than HD IL-2. Tyrosine 199-207 interleukin 2 Homo sapiens 140-144 3259228-5 1988 IL-2 was also found to induce the phosphorylation of proteins on tyrosine residues in short term cultures of human T lymphocytes. Tyrosine 65-73 interleukin 2 Homo sapiens 0-4 2435836-0 1987 Tyrosine phosphorylation of an interleukin 2 receptor-like protein in cells transformed by human T cell leukemia virus type I. Tyrosine 0-8 interleukin 2 Homo sapiens 31-44 32038633-1 2019 Interleukin-2 (IL-2) inducible T-cell kinase (ITK) is a non-receptor tyrosine kinase highly expressed in T-cell lineages and regulates multiple aspects of T-cell development and function, mainly through its function downstream of the T-cell receptor. Tyrosine 69-77 interleukin 2 Homo sapiens 0-13 30460991-8 2019 In contrast, in a more downstream readout, interleukin-2 expression, in response to immobilized CD3 and CD6 monoclonal antibodies, the C-terminal tyrosines were dominant. Tyrosine 146-155 interleukin 2 Homo sapiens 43-56 30455079-10 2018 NKL cells are dependent on interleukin (IL)-2 for survival and we show here for the first time that treatment with IL-2 induced tyrosine phosphorylation of STATs 1 through 6. Tyrosine 128-136 interleukin 2 Homo sapiens 27-45 30455079-10 2018 NKL cells are dependent on interleukin (IL)-2 for survival and we show here for the first time that treatment with IL-2 induced tyrosine phosphorylation of STATs 1 through 6. Tyrosine 128-136 interleukin 2 Homo sapiens 115-119