PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 18550772-4 2008 E2 treatment stimulated STAT5b tyrosine phosphorylation at the activating tyrosine Y699, resulting in increased STAT5-mediated transcriptional activity, which was inhibited by a Y669F STAT5b mutant. Tyrosine 31-39 signal transducer and activator of transcription 5B Homo sapiens 24-30 18550772-4 2008 E2 treatment stimulated STAT5b tyrosine phosphorylation at the activating tyrosine Y699, resulting in increased STAT5-mediated transcriptional activity, which was inhibited by a Y669F STAT5b mutant. Tyrosine 31-39 signal transducer and activator of transcription 5B Homo sapiens 184-190 18550772-4 2008 E2 treatment stimulated STAT5b tyrosine phosphorylation at the activating tyrosine Y699, resulting in increased STAT5-mediated transcriptional activity, which was inhibited by a Y669F STAT5b mutant. Tyrosine 74-82 signal transducer and activator of transcription 5B Homo sapiens 24-30 18550772-4 2008 E2 treatment stimulated STAT5b tyrosine phosphorylation at the activating tyrosine Y699, resulting in increased STAT5-mediated transcriptional activity, which was inhibited by a Y669F STAT5b mutant. Tyrosine 74-82 signal transducer and activator of transcription 5B Homo sapiens 184-190 18550772-6 2008 Both E2-induced STAT5b tyrosine phosphorylation and STAT5-mediated transcription were also inhibited by the ER antagonist ICI 182,780 and the c-Src inhibitor PP2, indicating additional requirements for the ER and c-Src kinase activity. Tyrosine 23-31 signal transducer and activator of transcription 5B Homo sapiens 16-22 17822672-7 2007 As STAT5b is implicated in several cancers, understanding how its activity is regulated through tyrosine and serine phosphorylation is vital for the development of potential novel cancer therapeutics. Tyrosine 96-104 signal transducer and activator of transcription 5B Homo sapiens 3-9 18430728-5 2008 The human receptor contains a STAT5B recruiting tyrosine motif (Tyr837/Tyr839) C-terminal to the box 1/2 region, which is absent in the mouse receptor. Tyrosine 48-56 signal transducer and activator of transcription 5B Homo sapiens 30-36 18430728-9 2008 Exchange by the murine counterpart (Cys815) allows recruitment of JAK2 by the human receptor and consequently activation of STAT5B independently of receptor tyrosine motifs. Tyrosine 157-165 signal transducer and activator of transcription 5B Homo sapiens 124-130 17823285-3 2007 PDGF-BB activated STAT-5B as measured by its tyrosine phosphorylation, DNA binding, and reporter gene activity. Tyrosine 45-53 signal transducer and activator of transcription 5B Homo sapiens 18-25 16584384-4 2006 The inhibitory effect of HNF4alpha on STAT5b transcription was associated with the inhibition of GH-stimulated STAT5b tyrosine phosphorylation and nuclear translocation. Tyrosine 118-126 signal transducer and activator of transcription 5B Homo sapiens 38-44 16862179-5 2007 NT also induced a time-dependent increase in EGFR Tyr(845) phosphorylation and phosphorylation of c-Src and signal transducer and activator of transcription 5b (Stat5b) (a downstream effector of Tyr(845)), events that were blocked by specific inhibition of c-Src (which mediates Tyr(845) phosphorylation of EGFR) or of EGFR. Tyrosine 195-198 signal transducer and activator of transcription 5B Homo sapiens 108-159 16862179-5 2007 NT also induced a time-dependent increase in EGFR Tyr(845) phosphorylation and phosphorylation of c-Src and signal transducer and activator of transcription 5b (Stat5b) (a downstream effector of Tyr(845)), events that were blocked by specific inhibition of c-Src (which mediates Tyr(845) phosphorylation of EGFR) or of EGFR. Tyrosine 195-198 signal transducer and activator of transcription 5B Homo sapiens 161-167 16862179-5 2007 NT also induced a time-dependent increase in EGFR Tyr(845) phosphorylation and phosphorylation of c-Src and signal transducer and activator of transcription 5b (Stat5b) (a downstream effector of Tyr(845)), events that were blocked by specific inhibition of c-Src (which mediates Tyr(845) phosphorylation of EGFR) or of EGFR. Tyrosine 195-198 signal transducer and activator of transcription 5B Homo sapiens 108-159 16862179-5 2007 NT also induced a time-dependent increase in EGFR Tyr(845) phosphorylation and phosphorylation of c-Src and signal transducer and activator of transcription 5b (Stat5b) (a downstream effector of Tyr(845)), events that were blocked by specific inhibition of c-Src (which mediates Tyr(845) phosphorylation of EGFR) or of EGFR. Tyrosine 195-198 signal transducer and activator of transcription 5B Homo sapiens 161-167 17997837-10 2007 RESULTS: Phosphospecific antibodies, mutational analysis, and in vitro kinase assays demonstrated that Brk specifically mediated STAT5b phosphorylation at the activating tyrosine, Y699. Tyrosine 170-178 signal transducer and activator of transcription 5B Homo sapiens 129-135 16772534-0 2006 Modulation of signal transducer and activator of transcription 5b activity in breast cancer cells by mutation of tyrosines within the transactivation domain. Tyrosine 113-122 signal transducer and activator of transcription 5B Homo sapiens 14-65 16772534-3 2006 Although phosphorylation of STAT5b on Y699 is required for activation, it was previously shown that in epidermal growth factor receptor (EGFR)-overexpressing cell lines, three tyrosines (Y725, Y740, and Y743) in the STAT5b transactivation domain are also phosphorylated upon epidermal growth factor stimulation. Tyrosine 176-185 signal transducer and activator of transcription 5B Homo sapiens 28-34 16584384-4 2006 The inhibitory effect of HNF4alpha on STAT5b transcription was associated with the inhibition of GH-stimulated STAT5b tyrosine phosphorylation and nuclear translocation. Tyrosine 118-126 signal transducer and activator of transcription 5B Homo sapiens 111-117 16584384-6 2006 HNF4alpha inhibition of STAT5b tyrosine phosphorylation was not reversed by pervanadate or by dominant-negative phosphotyrosine phosphatase 1B, suggesting that it does not result from an increase in STAT5b dephosphorylation. Tyrosine 31-39 signal transducer and activator of transcription 5B Homo sapiens 24-30 16584384-7 2006 Rather, HNF4alpha blocked GH-stimulated tyrosine phosphorylation of JAK2 (Janus kinase 2), a STAT5b tyrosine kinase. Tyrosine 40-48 signal transducer and activator of transcription 5B Homo sapiens 93-99 16464942-5 2006 However, the Src family of nonreceptor kinases [constitutively active v-src and epithelial growth factor-induced c-src] tyrosine-phosphorylated STAT5b(A630P). Tyrosine 120-128 signal transducer and activator of transcription 5B Homo sapiens 144-150 16464942-7 2006 CONCLUSIONS: The A630P mutation disrupts the src-homology-2 architecture such that: 1) mutant STAT5b most likely cannot dock to phospho-tyrosines on ligand-activated receptors; and 2) stable interactions with DNA are prevented. Tyrosine 136-145 signal transducer and activator of transcription 5B Homo sapiens 94-100 12621061-5 2003 These conformational differences may in part be due to differential effects of Prl and Src on Stat5b tyrosine phosphorylation, since Src induced several additional sites of tyrosine phosphorylation of Stat5b at residues other than Tyr-699, including Tyr-724 and Tyr-679. Tyrosine 173-181 signal transducer and activator of transcription 5B Homo sapiens 201-207 16303763-3 2006 In a cellular reconstitution model growth hormone robustly stimulated tyrosine phosphorylation and transcriptional activity of wild-type Stat5b while Stat5bA630P was minimally modified and did not promote reporter gene expression. Tyrosine 70-78 signal transducer and activator of transcription 5B Homo sapiens 137-143 15976008-6 2005 Introduction of ERalpha and treatment with E2 decreased EGF-induced tyrosine phosphorylation of STAT5b, basal and EGF-induced STAT5-mediated transcription, and EGF-stimulated DNA synthesis in these cells. Tyrosine 68-76 signal transducer and activator of transcription 5B Homo sapiens 96-102 15831712-5 2005 After GH stimulation, tyrosine phosphorylation of GHR, JAK2, STAT5b, and ERK1/2 increased 2- to 10-fold. Tyrosine 22-30 signal transducer and activator of transcription 5B Homo sapiens 61-67 15795318-6 2005 IL-3 treatment increases JAK2 activation and STAT5A and STAT5B tyrosine and serine phosphorylation and leads to cell cycle progression in adherent cells. Tyrosine 63-71 signal transducer and activator of transcription 5B Homo sapiens 56-62 15749807-3 2005 Previous studies in our laboratory have shown that GH induction of signal transducers and activators of transcription (STAT)5B tyrosine phosphorylation is inhibited by prolonged insulin treatment, probably via downregulation of GHR. Tyrosine 127-135 signal transducer and activator of transcription 5B Homo sapiens 119-126 14730712-6 2004 Stable transfection of EOC-20 cells with a dominant negative JAK2 mutant also blocked IL-3-induced tyrosine phosphorylation of JAK2, STAT5A, and STAT5B in microglia. Tyrosine 99-107 signal transducer and activator of transcription 5B Homo sapiens 145-151 16155412-5 2005 Imunoprecipitation and subsequent Western analysis showed that Jak2 leads to the tyrosine phosphorylation and activation of STAT5a or STAT5b under hypoxic conditions. Tyrosine 81-89 signal transducer and activator of transcription 5B Homo sapiens 134-140 15121872-7 2004 We show that phosphorylation of tyrosine 813 is required for the SH2 domain-containing adapter protein SH2-B beta to bind JAK2 and to enhance the activity of JAK2 and STAT5B. Tyrosine 32-40 signal transducer and activator of transcription 5B Homo sapiens 167-173 12621061-5 2003 These conformational differences may in part be due to differential effects of Prl and Src on Stat5b tyrosine phosphorylation, since Src induced several additional sites of tyrosine phosphorylation of Stat5b at residues other than Tyr-699, including Tyr-724 and Tyr-679. Tyrosine 101-109 signal transducer and activator of transcription 5B Homo sapiens 94-100 12621061-5 2003 These conformational differences may in part be due to differential effects of Prl and Src on Stat5b tyrosine phosphorylation, since Src induced several additional sites of tyrosine phosphorylation of Stat5b at residues other than Tyr-699, including Tyr-724 and Tyr-679. Tyrosine 250-253 signal transducer and activator of transcription 5B Homo sapiens 201-207 12621061-5 2003 These conformational differences may in part be due to differential effects of Prl and Src on Stat5b tyrosine phosphorylation, since Src induced several additional sites of tyrosine phosphorylation of Stat5b at residues other than Tyr-699, including Tyr-724 and Tyr-679. Tyrosine 250-253 signal transducer and activator of transcription 5B Homo sapiens 201-207 12429742-4 2003 We demonstrate that 1) activation of STAT5b by EGF requires overexpression of the EGFR, 2) co-overexpression of c-Src alone does not result in EGF-induced activation of STAT5b but enhances that seen in EGFR-overexpressing cells, and 3) EGF-induced tyrosine phosphorylation of STAT5b requires Tyr(845) of the EGFR. Tyrosine 248-256 signal transducer and activator of transcription 5B Homo sapiens 37-43 12429742-4 2003 We demonstrate that 1) activation of STAT5b by EGF requires overexpression of the EGFR, 2) co-overexpression of c-Src alone does not result in EGF-induced activation of STAT5b but enhances that seen in EGFR-overexpressing cells, and 3) EGF-induced tyrosine phosphorylation of STAT5b requires Tyr(845) of the EGFR. Tyrosine 292-295 signal transducer and activator of transcription 5B Homo sapiens 37-43 12429742-5 2003 Furthermore, the stable overexpression of a kinase-defective c-Src in the context of EGFR overexpression results in a decrease in the tyrosine phosphorylation of STAT5b in response to EGF and a more dramatic decrease in EGF-induced transcriptional activation of STAT5b, suggesting an integral role for c-Src in the physiological actions of STAT5b. Tyrosine 134-142 signal transducer and activator of transcription 5B Homo sapiens 162-168 12429742-7 2003 Finally, the use of site-specific tyrosine mutants demonstrates that EGF-induced phosphorylation of STAT5b involves not only tyrosine 699 of STAT5b, which is required for its transcriptional activation, but also three previously identified tyrosines in the C terminus of STAT5b (Tyr(725)/Tyr(740)/Tyr(743)). Tyrosine 34-42 signal transducer and activator of transcription 5B Homo sapiens 100-106 12429742-7 2003 Finally, the use of site-specific tyrosine mutants demonstrates that EGF-induced phosphorylation of STAT5b involves not only tyrosine 699 of STAT5b, which is required for its transcriptional activation, but also three previously identified tyrosines in the C terminus of STAT5b (Tyr(725)/Tyr(740)/Tyr(743)). Tyrosine 34-42 signal transducer and activator of transcription 5B Homo sapiens 141-147 12429742-7 2003 Finally, the use of site-specific tyrosine mutants demonstrates that EGF-induced phosphorylation of STAT5b involves not only tyrosine 699 of STAT5b, which is required for its transcriptional activation, but also three previously identified tyrosines in the C terminus of STAT5b (Tyr(725)/Tyr(740)/Tyr(743)). Tyrosine 34-42 signal transducer and activator of transcription 5B Homo sapiens 141-147 12429742-7 2003 Finally, the use of site-specific tyrosine mutants demonstrates that EGF-induced phosphorylation of STAT5b involves not only tyrosine 699 of STAT5b, which is required for its transcriptional activation, but also three previously identified tyrosines in the C terminus of STAT5b (Tyr(725)/Tyr(740)/Tyr(743)). Tyrosine 125-133 signal transducer and activator of transcription 5B Homo sapiens 100-106 12429742-7 2003 Finally, the use of site-specific tyrosine mutants demonstrates that EGF-induced phosphorylation of STAT5b involves not only tyrosine 699 of STAT5b, which is required for its transcriptional activation, but also three previously identified tyrosines in the C terminus of STAT5b (Tyr(725)/Tyr(740)/Tyr(743)). Tyrosine 125-133 signal transducer and activator of transcription 5B Homo sapiens 141-147 12429742-7 2003 Finally, the use of site-specific tyrosine mutants demonstrates that EGF-induced phosphorylation of STAT5b involves not only tyrosine 699 of STAT5b, which is required for its transcriptional activation, but also three previously identified tyrosines in the C terminus of STAT5b (Tyr(725)/Tyr(740)/Tyr(743)). Tyrosine 125-133 signal transducer and activator of transcription 5B Homo sapiens 141-147 12429742-7 2003 Finally, the use of site-specific tyrosine mutants demonstrates that EGF-induced phosphorylation of STAT5b involves not only tyrosine 699 of STAT5b, which is required for its transcriptional activation, but also three previously identified tyrosines in the C terminus of STAT5b (Tyr(725)/Tyr(740)/Tyr(743)). Tyrosine 240-249 signal transducer and activator of transcription 5B Homo sapiens 100-106 12429742-7 2003 Finally, the use of site-specific tyrosine mutants demonstrates that EGF-induced phosphorylation of STAT5b involves not only tyrosine 699 of STAT5b, which is required for its transcriptional activation, but also three previously identified tyrosines in the C terminus of STAT5b (Tyr(725)/Tyr(740)/Tyr(743)). Tyrosine 279-282 signal transducer and activator of transcription 5B Homo sapiens 100-106 12429742-7 2003 Finally, the use of site-specific tyrosine mutants demonstrates that EGF-induced phosphorylation of STAT5b involves not only tyrosine 699 of STAT5b, which is required for its transcriptional activation, but also three previously identified tyrosines in the C terminus of STAT5b (Tyr(725)/Tyr(740)/Tyr(743)). Tyrosine 288-291 signal transducer and activator of transcription 5B Homo sapiens 100-106 12429742-7 2003 Finally, the use of site-specific tyrosine mutants demonstrates that EGF-induced phosphorylation of STAT5b involves not only tyrosine 699 of STAT5b, which is required for its transcriptional activation, but also three previously identified tyrosines in the C terminus of STAT5b (Tyr(725)/Tyr(740)/Tyr(743)). Tyrosine 288-291 signal transducer and activator of transcription 5B Homo sapiens 100-106 11562369-8 2001 HNF3beta, in turn, inhibited STAT5b by a novel mechanism that leads to suppression of GH-inducible STAT5b tyrosine phosphorylation, DNA binding activity, and transcriptional activity. Tyrosine 106-114 signal transducer and activator of transcription 5B Homo sapiens 29-35 12089361-9 2002 The cooperation depends on the PRL-induced phosphorylation on Tyr(694) in Stat5A and Tyr(699) in Stat5B, as demonstrated using the Stat5AY694F and Stat5BY699F proteins. Tyrosine 62-65 signal transducer and activator of transcription 5B Homo sapiens 97-103 12089361-9 2002 The cooperation depends on the PRL-induced phosphorylation on Tyr(694) in Stat5A and Tyr(699) in Stat5B, as demonstrated using the Stat5AY694F and Stat5BY699F proteins. Tyrosine 85-88 signal transducer and activator of transcription 5B Homo sapiens 97-103 11751923-4 2002 Furthermore, EGF (but not GH) is able to activate tyrosine phosphorylation of a Tyr-699 mutant of STAT5b. Tyrosine 50-58 signal transducer and activator of transcription 5B Homo sapiens 98-104 11751923-4 2002 Furthermore, EGF (but not GH) is able to activate tyrosine phosphorylation of a Tyr-699 mutant of STAT5b. Tyrosine 80-83 signal transducer and activator of transcription 5B Homo sapiens 98-104 11781254-2 2002 Using human T cells, it was found that a recently discovered variant of the undecylprodigiosin family of antibiotics, PNU156804, previously shown to inhibit IL-2-induced cell proliferation, also blocks IL-2-mediated Jak3 auto-tyrosine phosphorylation, activation of Jak3 substrates signal transducers and activators of transcription (Stat) 5a and Stat5b, and extracellular regulated kinase 1 (Erk1) and Erk2 (p44/p42). Tyrosine 226-234 signal transducer and activator of transcription 5B Homo sapiens 347-353 11577084-4 2001 In transfection assays both Stat5 isoforms, Stat5a and Stat5b, were phosphorylated on tyrosine in response to IFN-gamma. Tyrosine 86-94 signal transducer and activator of transcription 5B Homo sapiens 55-61 11731617-1 2001 Signal transducer and activator of transcription 5b (STAT5b), the major liver-expressed STAT5 form, is phosphorylated on both tyrosine and serine in GH-stimulated cells. Tyrosine 126-134 signal transducer and activator of transcription 5B Homo sapiens 0-51 11731617-1 2001 Signal transducer and activator of transcription 5b (STAT5b), the major liver-expressed STAT5 form, is phosphorylated on both tyrosine and serine in GH-stimulated cells. Tyrosine 126-134 signal transducer and activator of transcription 5B Homo sapiens 53-59 11731617-2 2001 Although tyrosine phosphorylation is known to be critical for the dimerization, nuclear translocation, and activation of STAT5b DNA-binding and transcriptional activities, the effect of STAT5b serine phosphorylation is uncertain. Tyrosine 9-17 signal transducer and activator of transcription 5B Homo sapiens 121-127 11731617-4 2001 We additionally show that the serine kinase inhibitor H7 partially blocks the GH-stimulated formation of (Ser,Tyr)-diphosphorylated STAT5b without inhibiting STAT5b nuclear translocation. Tyrosine 110-113 signal transducer and activator of transcription 5B Homo sapiens 132-138 11562369-8 2001 HNF3beta, in turn, inhibited STAT5b by a novel mechanism that leads to suppression of GH-inducible STAT5b tyrosine phosphorylation, DNA binding activity, and transcriptional activity. Tyrosine 106-114 signal transducer and activator of transcription 5B Homo sapiens 99-105 10618497-4 1999 We here describe the selective tyrosine phosphorylation of STAT5b in Kym-1 cells in response to insulin. Tyrosine 31-39 signal transducer and activator of transcription 5B Homo sapiens 59-65 10847629-7 2000 Tyrosine phosphorylation of both Stat5a and Stat5b was induced by IL-6. Tyrosine 0-8 signal transducer and activator of transcription 5B Homo sapiens 44-50 11448122-2 2001 Leptin (50-200 nM) significantly increased tyrosine phosphorylation of STAT cytoplasmic transcription factors STAT3 and STAT5b in a dose-dependent manner and produced a gel-shift with STAT3- and STAT5-specific oligonucleotides. Tyrosine 43-51 signal transducer and activator of transcription 5B Homo sapiens 120-126 10830280-4 2000 Firstly, we show that both insulin and insulin-like growth factor I lead to tyrosine phosphorylation of Stat 5B, and this promotes binding of the transcription factor to the beta-casein promoter containing a Stat 5 binding site. Tyrosine 76-84 signal transducer and activator of transcription 5B Homo sapiens 104-111 10618497-5 1999 Blocking insulin signalling by HNMPA-(AM)(3), an insulin receptor kinase inhibitor, resulted in the loss of insulin-induced STAT5b tyrosine phosphorylation, whereas the inhibition of JAK2 by the JAK selective inhibitor tyrphostin AG490 had no effect. Tyrosine 131-139 signal transducer and activator of transcription 5B Homo sapiens 124-130 10205146-3 1999 Moreover, kinetic analysis of TPO-induced tyrosine phosphorylation of STAT demonstrated that STAT5B activation temporally correlated with the synthesis of PAF. Tyrosine 42-50 signal transducer and activator of transcription 5B Homo sapiens 93-99 10585430-7 1999 Mutation of GHR"s membrane-proximal tyrosine residues 333 and 338 to phenylalanine suppressed the inhibition by SOCS-3, but not by CIS, of GH signaling to STAT5b. Tyrosine 36-44 signal transducer and activator of transcription 5B Homo sapiens 155-161 10350046-2 1999 In addition to the previously demonstrated tyrosine phosphorylation, we show that serine and threonine phosphorylation of Stat5b is increased in response to PDGF stimulation. Tyrosine 43-51 signal transducer and activator of transcription 5B Homo sapiens 122-128 10585430-8 1999 SOCS/CIS proteins can thus inhibit GH signaling to STAT5b by three distinct mechanisms, distinguished by their molecular targets within the GHR-JAK2 signaling complex, as exemplified by SOCS-1 (direct JAK2 kinase inhibition), SOCS-3 (inhibition of JAK2 signaling via membrane-proximal GHR tyrosines 333 and 338), and CIS and SOCS-2 (inhibition via membrane-distal tyrosine(s)). Tyrosine 289-298 signal transducer and activator of transcription 5B Homo sapiens 51-57 10585430-8 1999 SOCS/CIS proteins can thus inhibit GH signaling to STAT5b by three distinct mechanisms, distinguished by their molecular targets within the GHR-JAK2 signaling complex, as exemplified by SOCS-1 (direct JAK2 kinase inhibition), SOCS-3 (inhibition of JAK2 signaling via membrane-proximal GHR tyrosines 333 and 338), and CIS and SOCS-2 (inhibition via membrane-distal tyrosine(s)). Tyrosine 289-297 signal transducer and activator of transcription 5B Homo sapiens 51-57 10373548-8 1999 Additionally, in lymphocytes expressing SOCS-3 but not CIS, IL-2-induced tyrosine phosphorylation of STAT5b was markedly reduced, while there was only a weak effect on IL-3-mediated STAT5b tyrosine phosphorylation. Tyrosine 73-81 signal transducer and activator of transcription 5B Homo sapiens 101-107 10205146-4 1999 PAF, in turn, induced a rapid tyrosine phosphorylation of STAT5B and PAF receptor blockade, by WEB 2170, preventing both TPO- and PAF-mediated STAT5B activation. Tyrosine 30-38 signal transducer and activator of transcription 5B Homo sapiens 58-64 10205146-4 1999 PAF, in turn, induced a rapid tyrosine phosphorylation of STAT5B and PAF receptor blockade, by WEB 2170, preventing both TPO- and PAF-mediated STAT5B activation. Tyrosine 30-38 signal transducer and activator of transcription 5B Homo sapiens 143-149 9657743-0 1998 Erythropoietin induces tyrosine phosphorylation of Jak2, STAT5A, and STAT5B in primary cultured human erythroid precursors. Tyrosine 23-31 signal transducer and activator of transcription 5B Homo sapiens 69-75 10068671-9 1999 IFN-alpha enhanced tyrosine phosphorylation of STAT1, STAT3, STAT4, STAT5a, and STAT5b. Tyrosine 19-27 signal transducer and activator of transcription 5B Homo sapiens 80-86 9657743-2 1998 We found that erythropoietin induces tyrosine phosphorylation of Jak2, STAT5A, and STAT5B. Tyrosine 37-45 signal transducer and activator of transcription 5B Homo sapiens 83-89 9544991-8 1998 The dominant negative mutant of SHP-2 was found to inhibit the induction of tyrosine phosphorylation and DNA-binding activity of m-Stat5a, m-Stat5b, and the carboxyl-terminal deletion variant m-Stat5adelta749, as well as the transactivation potential of m-Stat5a and m-Stat5b. Tyrosine 76-84 signal transducer and activator of transcription 5B Homo sapiens 141-147 9122188-2 1997 Stat5b-Ct is tyrosine phosphorylated by purified IR kinase domain in vitro. Tyrosine 13-21 signal transducer and activator of transcription 5B Homo sapiens 0-6 9422769-12 1998 By the use of specific antibodies, it was determined that only STAT5B, and not STAT5A, was tyrosine phosphorylated in GM-CSF-treated neutrophils. Tyrosine 91-99 signal transducer and activator of transcription 5B Homo sapiens 63-69 9428692-4 1997 Further, we found that insulin promotes rapid tyrosine phosphorylation of endogenous Stat 5B in 293 EBNA cells overexpressing insulin receptor and in NHIR cells. Tyrosine 46-54 signal transducer and activator of transcription 5B Homo sapiens 85-92 9398404-5 1997 IL-2 and IL-7 were equivalent in their ability to induce tyrosine phosphorylation of STAT5A and STAT5B and to facilitate binding of these STATs to an immobilized GAS element. Tyrosine 57-65 signal transducer and activator of transcription 5B Homo sapiens 96-102 9182578-5 1997 We now report that IL2 stimulated marked phosphorylation of serine and tyrosine residues of both Stat5a and Stat5b in human T lymphocytes and in several IL2-responsive lymphocytic cell lines. Tyrosine 71-79 signal transducer and activator of transcription 5B Homo sapiens 108-114 9164861-3 1997 Furthermore it stimulated tyrosine phosphorylation of two isoforms of the transcriptional activator STAT5, STAT5a and STAT5b. Tyrosine 26-34 signal transducer and activator of transcription 5B Homo sapiens 118-124 9122188-3 1997 Insulin stimulates tyrosine phosphorylation of overexpressed Stat5b-Ct and endogenous Stat5 in cells overexpressing IR. Tyrosine 19-27 signal transducer and activator of transcription 5B Homo sapiens 61-67 8790376-3 1996 Ligand activation of c-ErbB induced the tyrosine phosphorylation, DNA-binding, and reporter gene transcription of Stat 5b in erythroblasts. Tyrosine 40-48 signal transducer and activator of transcription 5B Homo sapiens 114-121 33248444-11 2021 In addition, these cells showed enhanced tyrosine phosphorylation and transcriptional activity of signal transducer and activator of transcription 5b (STAT5b) and increased IGF-1 expression induced by GH. Tyrosine 41-49 signal transducer and activator of transcription 5B Homo sapiens 98-149 8631883-7 1996 Moreover, we demonstrated that human Stat5 activation is also dependent on Tyr-694 in Stat5A and Tyr-699 in Stat5B, indicating that these tyrosines are required for dimerization. Tyrosine 97-100 signal transducer and activator of transcription 5B Homo sapiens 108-114 8631883-7 1996 Moreover, we demonstrated that human Stat5 activation is also dependent on Tyr-694 in Stat5A and Tyr-699 in Stat5B, indicating that these tyrosines are required for dimerization. Tyrosine 138-147 signal transducer and activator of transcription 5B Homo sapiens 108-114 33248444-11 2021 In addition, these cells showed enhanced tyrosine phosphorylation and transcriptional activity of signal transducer and activator of transcription 5b (STAT5b) and increased IGF-1 expression induced by GH. Tyrosine 41-49 signal transducer and activator of transcription 5B Homo sapiens 151-157 29844444-4 2018 These STAT5B missense mutants are robustly tyrosine phosphorylated upon stimulation, but are unable to nuclear localize, or fail to bind canonical STAT5B DNA response elements. Tyrosine 43-51 signal transducer and activator of transcription 5B Homo sapiens 6-12 30835430-6 2019 Amino acid 566 also determines the affinity for a tyrosine-phosphorylated peptide derived from the EPO receptor for STAT5a and STAT5b, demonstrating the functional relevance of the STAT5 linker domain for the adjacent SH2 domain. Tyrosine 50-58 signal transducer and activator of transcription 5B Homo sapiens 127-133 31861239-1 2019 Signal transducers and activators of transcription 5A and 5B (STAT5A and STAT5B) are crucial downstream effectors of tyrosine kinase oncogenes (TKO) such as BCR-ABL in chronic myeloid leukemia (CML) and FLT3-ITD in acute myeloid leukemia (AML). Tyrosine 117-125 signal transducer and activator of transcription 5B Homo sapiens 73-79 20962506-0 2011 A novel Y332C missense mutation in the intracellular domain of the human growth hormone receptor does not alter STAT5b signaling: redundancy of GHR intracellular tyrosines involved in STAT5b signaling. Tyrosine 162-171 signal transducer and activator of transcription 5B Homo sapiens 184-190 27035235-6 2016 Immunoprecipitation analysis revealed tyrosine phosphorylation of STAT5b in pancreatic cancer cells. Tyrosine 38-46 signal transducer and activator of transcription 5B Homo sapiens 66-72 28400581-5 2017 The prodrug Pomstafib-2 selectively inhibits tyrosine phosphorylation of STAT5b in human leukaemia cells and induces apoptosis in a STAT5-dependent manner. Tyrosine 45-53 signal transducer and activator of transcription 5B Homo sapiens 73-79 23160480-6 2013 Unlike STAT5b(A630P), which showed minimal GH-induced tyrosine phosphorylation and no transcriptional activity, STAT5b(F646S) became tyrosine phosphorylated after GH treatment and could function as a GH-activated transcription factor, although to a substantially lesser extent than STAT5b(WT). Tyrosine 133-141 signal transducer and activator of transcription 5B Homo sapiens 112-118 23160480-6 2013 Unlike STAT5b(A630P), which showed minimal GH-induced tyrosine phosphorylation and no transcriptional activity, STAT5b(F646S) became tyrosine phosphorylated after GH treatment and could function as a GH-activated transcription factor, although to a substantially lesser extent than STAT5b(WT). Tyrosine 133-141 signal transducer and activator of transcription 5B Homo sapiens 112-118 22442148-2 2012 Maximum transcriptional activation of Stat5b requires both tyrosine and serine phosphorylation. Tyrosine 59-67 signal transducer and activator of transcription 5B Homo sapiens 38-44 20962506-1 2011 BACKGROUND: The growth hormone receptor (GHR), upon binding with GH, induces JAK2-mediated phosphorylation of GHR intracellular tyrosines, which then recruit STAT5b. Tyrosine 128-137 signal transducer and activator of transcription 5B Homo sapiens 158-164 20962506-3 2011 OBJECTIVE: To evaluate and compare the role of a novel Y332C GHR variant identified in a patient with short stature to the other GHR intracellular tyrosines in the GHR-STAT5b signaling process. Tyrosine 147-156 signal transducer and activator of transcription 5B Homo sapiens 168-174 20962506-5 2011 However, GH-induced STAT5b activation was specifically abrogated in an hGHR variant in which all 7 tyrosines were inactivated (MYF). Tyrosine 99-108 signal transducer and activator of transcription 5B Homo sapiens 20-26 20962506-6 2011 When hGHR variants carrying single intracellular tyrosines were evaluated, STAT5b activation was comparable to that of wild-type hGHR only with variants carrying Y534, Y566 or Y627. Tyrosine 49-58 signal transducer and activator of transcription 5B Homo sapiens 75-81 20962506-7 2011 CONCLUSION: We provide evidence that in human GHR, 3 intracellular tyrosines are critical and redundant in the GH-induced STAT5b signaling process. Tyrosine 67-76 signal transducer and activator of transcription 5B Homo sapiens 122-128 19438593-8 2009 We demonstrate further that c-Src can phosphorylate signal transducer and activator of transcription 5b (STAT5b) on tyrosine 699 and that c-Src and STAT5b co-associate during B lymphocyte proliferation. Tyrosine 116-124 signal transducer and activator of transcription 5B Homo sapiens 52-103 21166888-6 2010 Human recombinant GH stimulated JAK2 and STAT5b tyrosine phosphorylation and IGF-I production in a concentration-dependent manner. Tyrosine 48-56 signal transducer and activator of transcription 5B Homo sapiens 41-47 20433855-4 2010 [D-Ser(2), Leu(5), Thr(6)]-enkephalin-exposure of HEK293 cells, expressing stably the delta-opioid receptor (delta-OR), leads to receptor-dependent STAT5B tyrosine phosphorylation and transcriptional activation. Tyrosine 155-163 signal transducer and activator of transcription 5B Homo sapiens 148-154 19349617-4 2009 Similarly, 15(S)-HETE activated tyrosine phosphorylation of STAT-5B in a time-dependent manner. Tyrosine 32-40 signal transducer and activator of transcription 5B Homo sapiens 60-67 19438593-8 2009 We demonstrate further that c-Src can phosphorylate signal transducer and activator of transcription 5b (STAT5b) on tyrosine 699 and that c-Src and STAT5b co-associate during B lymphocyte proliferation. Tyrosine 116-124 signal transducer and activator of transcription 5B Homo sapiens 105-111 19438593-8 2009 We demonstrate further that c-Src can phosphorylate signal transducer and activator of transcription 5b (STAT5b) on tyrosine 699 and that c-Src and STAT5b co-associate during B lymphocyte proliferation. Tyrosine 116-124 signal transducer and activator of transcription 5B Homo sapiens 148-154