PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 31963765-2 2020 STAT3 and STAT5 are common targets for different tyrosine kinase oncogenes (TKOs). Tyrosine 49-57 signal transducer and activator of transcription 5A Homo sapiens 10-15 33075386-6 2021 Western blotting confirmed that tyrosine phosphorylation in STAT5, a substrate of ABL1, was enhanced, and the novel mutation was proved to be a gain-of-function mutation. Tyrosine 32-40 signal transducer and activator of transcription 5A Homo sapiens 60-65 32041920-0 2019 The Role of STAT5 in Tyrosine Kinase Inhibitor (IMATINIB) Resistance in CML Patients. Tyrosine 21-29 signal transducer and activator of transcription 5A Homo sapiens 12-17 31861720-7 2019 Signal transducer and activator of transcription-3 (STAT3) and STAT5 are aberrantly activated through tyrosine phosphorylation in a wide variety of cancer types, including EOC. Tyrosine 102-110 signal transducer and activator of transcription 5A Homo sapiens 63-68 31861239-1 2019 Signal transducers and activators of transcription 5A and 5B (STAT5A and STAT5B) are crucial downstream effectors of tyrosine kinase oncogenes (TKO) such as BCR-ABL in chronic myeloid leukemia (CML) and FLT3-ITD in acute myeloid leukemia (AML). Tyrosine 117-125 signal transducer and activator of transcription 5A Homo sapiens 62-68 32041920-7 2019 This review summarizes the role of STAT5 in tyrosine kinase inhibitor resistance in CML patients. Tyrosine 44-52 signal transducer and activator of transcription 5A Homo sapiens 35-40 30835430-6 2019 Amino acid 566 also determines the affinity for a tyrosine-phosphorylated peptide derived from the EPO receptor for STAT5a and STAT5b, demonstrating the functional relevance of the STAT5 linker domain for the adjacent SH2 domain. Tyrosine 50-58 signal transducer and activator of transcription 5A Homo sapiens 116-122 31172340-8 2019 Our data revealed that TMPRSS6 and alpha 1-antitrypsin levels decreased due to fractalkine treatment, as well as the activity of NFkappaB pathway and the tyrosine phosphorylation of STAT5 factor. Tyrosine 154-162 signal transducer and activator of transcription 5A Homo sapiens 182-187 31085588-4 2019 IL-2-induced tyrosine phosphorylation of STAT5 (pSTAT5) was proportional to CD25 levels on human CD4+ T cells and YT human NK cell line, directly demonstrating that CD25 promotes IL-2R signaling. Tyrosine 13-21 signal transducer and activator of transcription 5A Homo sapiens 41-46 29939941-4 2019 He presents with preserved STAT5 tyrosine phosphorylation in response to IL-15 stimulation but not in response to IL-2 and IL-7, resulting in the NK phenotype. Tyrosine 33-41 signal transducer and activator of transcription 5A Homo sapiens 27-32 30835430-6 2019 Amino acid 566 also determines the affinity for a tyrosine-phosphorylated peptide derived from the EPO receptor for STAT5a and STAT5b, demonstrating the functional relevance of the STAT5 linker domain for the adjacent SH2 domain. Tyrosine 50-58 signal transducer and activator of transcription 5A Homo sapiens 116-121 29200404-6 2018 Persistent and enhanced levels of STAT5BN642H tyrosine phosphorylation in transformed CD8+ T cells led to profound changes in gene expression that were accompanied by alterations in DNA methylation at potential histone methyltransferase EZH2-binding sites. Tyrosine 46-54 signal transducer and activator of transcription 5A Homo sapiens 34-39 30818760-9 2019 We emphasize that a single O-GlcNAc modification is essential to promote development of neoplastic cell growth through enhancing STAT5A tyrosine phosphorylation. Tyrosine 136-144 signal transducer and activator of transcription 5A Homo sapiens 129-135 30818760-10 2019 Inhibition of O-GlcNAcylation of STAT5A on threonine 92 lowers tyrosine phosphorylation of oncogenic STAT5A and ablates malignant transformation. Tyrosine 63-71 signal transducer and activator of transcription 5A Homo sapiens 33-39 30818760-10 2019 Inhibition of O-GlcNAcylation of STAT5A on threonine 92 lowers tyrosine phosphorylation of oncogenic STAT5A and ablates malignant transformation. Tyrosine 63-71 signal transducer and activator of transcription 5A Homo sapiens 101-107 30157040-6 2018 RITA decreased STAT5 tyrosine phosphorylation, although it did not inhibit phosphorylation of the direct BCR-ABL substrate CrkL. Tyrosine 21-29 signal transducer and activator of transcription 5A Homo sapiens 15-20 29857127-5 2018 Interestingly, FT also suppressed constitutive STAT3 (tyrosine residue 705 and serine residue 727) and STAT5 (tyrosine residue 694/699) activation, which correlated with the suppression of the upstream kinases (JAK1, JAK2, and c-Src) in MM cells, and this effect was found to be mediated via an increased production of reactive oxygen species (ROS) due to GSH/GSSG imbalance. Tyrosine 110-118 signal transducer and activator of transcription 5A Homo sapiens 103-108 29249817-10 2018 In fact, tyrosine phosphorylation of STAT5 was inhibited by R763 at 10 nM. Tyrosine 9-17 signal transducer and activator of transcription 5A Homo sapiens 37-42 28982698-6 2017 We found that multiple PDC subunits interact with hormone-activated STAT5A in a dose- and time-dependent manner that coincides with tyrosine phosphorylation of STAT5. Tyrosine 132-140 signal transducer and activator of transcription 5A Homo sapiens 68-74 28982698-6 2017 We found that multiple PDC subunits interact with hormone-activated STAT5A in a dose- and time-dependent manner that coincides with tyrosine phosphorylation of STAT5. Tyrosine 132-140 signal transducer and activator of transcription 5A Homo sapiens 68-73 28074064-0 2017 O-GlcNAcylation of STAT5 controls tyrosine phosphorylation and oncogenic transcription in STAT5-dependent malignancies. Tyrosine 34-42 signal transducer and activator of transcription 5A Homo sapiens 19-24 28074064-0 2017 O-GlcNAcylation of STAT5 controls tyrosine phosphorylation and oncogenic transcription in STAT5-dependent malignancies. Tyrosine 34-42 signal transducer and activator of transcription 5A Homo sapiens 90-95 28074064-2 2017 Upon cytokine stimulation, STAT5 tyrosine phosphorylation (pYSTAT5) is transient, while in diverse neoplastic cells persistent overexpression and enhanced pYSTAT5 are frequently found. Tyrosine 33-41 signal transducer and activator of transcription 5A Homo sapiens 27-32 28074064-5 2017 The expression of a mutated hyperactive gain-of-function (GOF) STAT5 without O-GlcNAcylation resulted in decreased tyrosine phosphorylation, oligomerization and transactivation potential and complete loss of oncogenic transformation capacity. Tyrosine 115-123 signal transducer and activator of transcription 5A Homo sapiens 63-68 28074064-7 2017 Our data show that O-GlcNAcylation of STAT5 is an important process that contributes to oncogenic transcription through enhanced STAT5 tyrosine phosphorylation and oligomerization driving myeloid transformation. Tyrosine 135-143 signal transducer and activator of transcription 5A Homo sapiens 38-43 28074064-7 2017 Our data show that O-GlcNAcylation of STAT5 is an important process that contributes to oncogenic transcription through enhanced STAT5 tyrosine phosphorylation and oligomerization driving myeloid transformation. Tyrosine 135-143 signal transducer and activator of transcription 5A Homo sapiens 129-134 28400581-5 2017 The prodrug Pomstafib-2 selectively inhibits tyrosine phosphorylation of STAT5b in human leukaemia cells and induces apoptosis in a STAT5-dependent manner. Tyrosine 45-53 signal transducer and activator of transcription 5A Homo sapiens 73-78 28468777-9 2017 STAT3/STAT5 activation depends on tyrosine phosphorylation, mediated by several upstream TKs. Tyrosine 34-42 signal transducer and activator of transcription 5A Homo sapiens 6-11 28629523-3 2017 In the study of the cancer cell lines in vitro, rhEpo accelerated the cancer cell growth, whereas the EMP9 inhibited the cell growth along with the inhibition of STAT5 tyrosine phosphorylation. Tyrosine 168-176 signal transducer and activator of transcription 5A Homo sapiens 162-167 28287479-5 2017 In addition, a few STAT5 interacting proteins have been identified as positive regulators of STAT5 that alter serine and tyrosine phosphorylation of STAT5 while other proteins have been identified as negative regulators of STAT5 via dephosphorylation. Tyrosine 121-129 signal transducer and activator of transcription 5A Homo sapiens 19-24 28287479-5 2017 In addition, a few STAT5 interacting proteins have been identified as positive regulators of STAT5 that alter serine and tyrosine phosphorylation of STAT5 while other proteins have been identified as negative regulators of STAT5 via dephosphorylation. Tyrosine 121-129 signal transducer and activator of transcription 5A Homo sapiens 93-98 28287479-5 2017 In addition, a few STAT5 interacting proteins have been identified as positive regulators of STAT5 that alter serine and tyrosine phosphorylation of STAT5 while other proteins have been identified as negative regulators of STAT5 via dephosphorylation. Tyrosine 121-129 signal transducer and activator of transcription 5A Homo sapiens 93-98 28287479-5 2017 In addition, a few STAT5 interacting proteins have been identified as positive regulators of STAT5 that alter serine and tyrosine phosphorylation of STAT5 while other proteins have been identified as negative regulators of STAT5 via dephosphorylation. Tyrosine 121-129 signal transducer and activator of transcription 5A Homo sapiens 93-98 27752093-1 2016 STAT5 is an essential transcription factor in hematopoiesis, which is activated through tyrosine phosphorylation in response to cytokine stimulation. Tyrosine 88-96 signal transducer and activator of transcription 5A Homo sapiens 0-5 27936140-8 2016 Based on the inhibitory effect of WHI-P131, a selective drug of JAK3 kinase activity, we concluded that in quiescent primary T lymphocytes, the constitutive STAT3 and the IL-2-induced prolonged STAT5 activity (assayed by tyrosine phosphorylation) is mostly JAK3-independent. Tyrosine 221-229 signal transducer and activator of transcription 5A Homo sapiens 194-199 27312066-9 2016 Fluorescence immunohistochemistry values for nuclear-localized and tyrosine-phosphorylated Stat5a/b computed by each platform on a cohort of 323 breast cancer cases revealed high concordance after linear calibration, a finding confirmed on an independent 382 case cohort, with concordance correlation coefficients >0.98. Tyrosine 67-75 signal transducer and activator of transcription 5A Homo sapiens 91-97 24836440-5 2014 Although BCR-ABL induces STAT5-tyrosine phosphorylation independent of JAK2-kinase activity, BCR-ABL is less efficient in inducing active STAT5A:STAT5B-heterodimerization than IL-3, leaving constitutive STAT5A and STAT5B-homodimerization unaffected. Tyrosine 31-39 signal transducer and activator of transcription 5A Homo sapiens 25-30 26059750-9 2016 This brings the two kinase domains into position for trans-activation and initiates tyrosine phosphorylation of the receptor cytoplasmic domain and other substrates such as STAT5, the key transcription factor mediating most genomic actions of GH. Tyrosine 84-92 signal transducer and activator of transcription 5A Homo sapiens 173-178 26702099-4 2016 We demonstrate that in the absence of thrombopoietin (TPO), tyrosine-unphosphorylated STAT5 (uSTAT5) is present in the nucleus where it colocalizes with CTCF and represses a megakaryocytic transcriptional program. Tyrosine 60-68 signal transducer and activator of transcription 5A Homo sapiens 86-91 30695496-3 2016 This leads to activation of tyrosine kinase JAK2, which phosphory- lates tyrosine-containing sites located in the cytoplasmic domain of the receptor, resulting in stimulation of activity of phosphatidylinositol-3-kinase, the transcription factors STAT3 and STAT5, phosphatase SHP2, and mitogen-activated protein kinase. Tyrosine 28-36 signal transducer and activator of transcription 5A Homo sapiens 257-262 25821217-6 2015 Finally, we found that STAT5 is recruited to the IL-25R in a ligand-dependent manner through unique tyrosine residues on IL-17RB. Tyrosine 100-108 signal transducer and activator of transcription 5A Homo sapiens 23-28 25153147-4 2014 Using reporter assays and site-directed mutagenesis we show that the frog LepR signals via signal transducer and activator of transcription (STAT) 3 and STAT5 through evolutionarily conserved tyrosine residues in the LepR cytoplasmic domain. Tyrosine 192-200 signal transducer and activator of transcription 5A Homo sapiens 153-158 24952131-4 2014 Our results show that PDTC downregulates prolactin receptor levels, and inhibits prolactin-induced Stat5 tyrosine phosphorylation and beta-casein expression. Tyrosine 105-113 signal transducer and activator of transcription 5A Homo sapiens 99-104 27076853-5 2016 Through data mining a published transcriptomic database of UBUCs (GSE32894), it identified Colony Stimulating Factor 2 (CSF2) as the stepwise upregulated gene of much significance among those related to the positive regulation of tyrosine phosphorylation of STAT5 (GO:0042523). Tyrosine 230-238 signal transducer and activator of transcription 5A Homo sapiens 258-263 26911335-3 2016 RESULTS: We found that RES suppressed both constitutive STAT3 (tyrosine residue 705 and serine residue 727) and STAT5 (tyrosine residue 694 and 699) activation, which correlated with the suppression of the upstream kinases (JAK1, JAK2, and c-Src) in RCC. Tyrosine 119-127 signal transducer and activator of transcription 5A Homo sapiens 112-117 25885255-2 2015 STAT5 is a BCR-ABL substrate and persistently activated by tyrosine phosphorylation in CML cells. Tyrosine 59-67 signal transducer and activator of transcription 5A Homo sapiens 0-5 25885255-8 2015 As a consequence of the complex formation between tyrosine-phosphorylated SFK and the SH2 domain of STAT5A, the dimerization of STAT5A is impaired. Tyrosine 50-58 signal transducer and activator of transcription 5A Homo sapiens 100-106 25885255-8 2015 As a consequence of the complex formation between tyrosine-phosphorylated SFK and the SH2 domain of STAT5A, the dimerization of STAT5A is impaired. Tyrosine 50-58 signal transducer and activator of transcription 5A Homo sapiens 128-134 25987027-9 2015 Western blotting analysis further identified that ASP markedly sensitized K562 cells to exogenous erythropoietin (EPO) by activating EPO-induced JAK2/ STAT5 tyrosine phosphorylation, thus augmenting the EPO-mediated JAK2/STAT5 signaling pathway. Tyrosine 157-165 signal transducer and activator of transcription 5A Homo sapiens 151-156 24821382-1 2014 Rapid increases in the tyrosine phosphorylation of signal transducers and activators of transcription 5 (STAT5) proteins have been extensively documented in cells stimulated with cytokines and growth factors. Tyrosine 23-31 signal transducer and activator of transcription 5A Homo sapiens 51-103 24821382-1 2014 Rapid increases in the tyrosine phosphorylation of signal transducers and activators of transcription 5 (STAT5) proteins have been extensively documented in cells stimulated with cytokines and growth factors. Tyrosine 23-31 signal transducer and activator of transcription 5A Homo sapiens 105-110 24821382-3 2014 To the best of our knowledge, the present study demonstrated for first time that stimulation of a glioblastoma multiforme (GBM) cell line (U87-MG) with hepatocyte growth factor (HGF) resulted in the phosphorylation of STAT5 at Tyr-694/699 and nuclear translocation of STAT5. Tyrosine 227-230 signal transducer and activator of transcription 5A Homo sapiens 218-223 23980167-5 2013 In vitro analysis using hepatocytes demonstrated that SIRT1 suppresses GH-dependent IGF-I expression, accompanied by decreased tyrosine phosphorylation on signal transducer and activator of transcription (STAT) 5. Tyrosine 127-135 signal transducer and activator of transcription 5A Homo sapiens 155-212 24263804-2 2014 STAT5 can be phosphorylated at three positions, on a tyrosine and on the two serines S725 and S779. Tyrosine 53-61 signal transducer and activator of transcription 5A Homo sapiens 0-5 23980167-8 2013 Knockdown of SIRT1 enhanced the acetylation and GH-induced tyrosine phosphorylation of STAT5, as well as the GH-induced interaction of the GH receptor with STAT5. Tyrosine 59-67 signal transducer and activator of transcription 5A Homo sapiens 87-92 23600834-4 2013 Basal and GM-CSF-primed neutrophil bacterial killing and signal transducer and activator of transcription 5 (STAT5) tyrosine phosphorylation (pSTAT5) were measured by flow cytometry. Tyrosine 116-124 signal transducer and activator of transcription 5A Homo sapiens 57-107 24040307-9 2013 Pim-1 inhibition also decreased phosphorylation of FLT3 at tyrosine 591 and of STAT5, and expression of Pim-1 itself, consistent with inhibition of the FLT3-ITD-STAT5 signaling pathway. Tyrosine 59-67 signal transducer and activator of transcription 5A Homo sapiens 161-166 21868263-13 2013 In p.Arg925Ser and p.Ala677Thr/p.Val722Ile, functional analyses showed abnormal JAK3 and STAT5 tyrosine phosphorylation and a reduction of JAK3/STAT5 interaction. Tyrosine 95-103 signal transducer and activator of transcription 5A Homo sapiens 89-94 23600834-4 2013 Basal and GM-CSF-primed neutrophil bacterial killing and signal transducer and activator of transcription 5 (STAT5) tyrosine phosphorylation (pSTAT5) were measured by flow cytometry. Tyrosine 116-124 signal transducer and activator of transcription 5A Homo sapiens 109-114 23596048-5 2013 The Y665F and N642H mutant constructs increased the transcriptional activity of STAT5 and tyrosine (Y694) phosphorylation, which was also observed in patient samples. Tyrosine 90-98 signal transducer and activator of transcription 5A Homo sapiens 80-85 25509109-4 2013 We observed phosphorylation of STAT5 no earlier than 5 h after PHA stimulation and the maximum phosphorylation was detected following 24 h. Exogenous IL-2 induced high level of STAT5 phosphorylation in the competent HBL as early as at 30 min and this level of STAT5 phosphorylation kept in the next 24-48 h. The correlation between alterations in tyrosine phosphorylation level of STAT5 and the expression of CD25 has been established. Tyrosine 347-355 signal transducer and activator of transcription 5A Homo sapiens 31-36 24751667-11 2013 Activating phosphorylation of ERK and STAT5 due to erythropoietin was practically prevented by ortho- or meta-tyrosine treatment. Tyrosine 110-118 signal transducer and activator of transcription 5A Homo sapiens 38-43 23332799-4 2013 We demonstrate here that stimulation of MDA-MB-231 breast cancer cells with 100 muM OLA induces Stat5 phosphorylation at Tyr-694 and an increase of Stat5-DNA complex formation. Tyrosine 121-124 signal transducer and activator of transcription 5A Homo sapiens 96-101 25509109-4 2013 We observed phosphorylation of STAT5 no earlier than 5 h after PHA stimulation and the maximum phosphorylation was detected following 24 h. Exogenous IL-2 induced high level of STAT5 phosphorylation in the competent HBL as early as at 30 min and this level of STAT5 phosphorylation kept in the next 24-48 h. The correlation between alterations in tyrosine phosphorylation level of STAT5 and the expression of CD25 has been established. Tyrosine 347-355 signal transducer and activator of transcription 5A Homo sapiens 177-182 25509109-4 2013 We observed phosphorylation of STAT5 no earlier than 5 h after PHA stimulation and the maximum phosphorylation was detected following 24 h. Exogenous IL-2 induced high level of STAT5 phosphorylation in the competent HBL as early as at 30 min and this level of STAT5 phosphorylation kept in the next 24-48 h. The correlation between alterations in tyrosine phosphorylation level of STAT5 and the expression of CD25 has been established. Tyrosine 347-355 signal transducer and activator of transcription 5A Homo sapiens 177-182 25509109-4 2013 We observed phosphorylation of STAT5 no earlier than 5 h after PHA stimulation and the maximum phosphorylation was detected following 24 h. Exogenous IL-2 induced high level of STAT5 phosphorylation in the competent HBL as early as at 30 min and this level of STAT5 phosphorylation kept in the next 24-48 h. The correlation between alterations in tyrosine phosphorylation level of STAT5 and the expression of CD25 has been established. Tyrosine 347-355 signal transducer and activator of transcription 5A Homo sapiens 177-182 22297723-6 2012 Importantly, the extent to which AKT, extracellular signal-regulated kinase and Stat5 signaling pathways are activated via the seven intracellular tyrosines in KITD814V impacts the latency of MPD and severity of the disease. Tyrosine 147-156 signal transducer and activator of transcription 5A Homo sapiens 80-85 23036105-2 2012 In breast cancer, loss of nuclear localized and tyrosine phosphorylated Stat5a/b is associated with poor prognosis and increased risk of antiestrogen therapy failure. Tyrosine 48-56 signal transducer and activator of transcription 5A Homo sapiens 72-78 21716071-4 2011 Phosphorylation of the transcription factor STAT5 on the regulatory serine S726 and the key tyrosine Y694 was monitored by intracellular flow cytometry. Tyrosine 92-100 signal transducer and activator of transcription 5A Homo sapiens 44-49 22528658-3 2012 IL-5 binding to IL-5R on target cells induces rapid tyrosine phosphorylation and activation of various cellular proteins, including JAK1/JAK2 and STAT1/STAT5. Tyrosine 52-60 signal transducer and activator of transcription 5A Homo sapiens 152-157 22411868-8 2012 We identified tyrosines 589 and 591 of FLT3-ITD to be essential for SRC binding and subsequent STAT5 activation. Tyrosine 14-23 signal transducer and activator of transcription 5A Homo sapiens 95-100 21678418-5 2012 Enforced activation of RhoA in MECs cultured on BM suppresses prolactin receptor levels, and prevents prolactin-induced Stat5 tyrosine phosphorylation and beta-casein expression. Tyrosine 126-134 signal transducer and activator of transcription 5A Homo sapiens 120-125 21622220-1 2011 Persistent tyrosine phosphorylation of Stat3 and Stat5 is associated with oncogenic activity. Tyrosine 11-19 signal transducer and activator of transcription 5A Homo sapiens 49-54 21617857-9 2011 The overexpression of DN-STAT5 significantly inhibited EGF-induced COX-2 expression, and we found that EGF induced the tyrosine phosphorylation of STAT5 and up- regulated COX-2 expression. Tyrosine 119-127 signal transducer and activator of transcription 5A Homo sapiens 25-30 21617857-9 2011 The overexpression of DN-STAT5 significantly inhibited EGF-induced COX-2 expression, and we found that EGF induced the tyrosine phosphorylation of STAT5 and up- regulated COX-2 expression. Tyrosine 119-127 signal transducer and activator of transcription 5A Homo sapiens 147-152 21576635-0 2011 Loss of nuclear localized and tyrosine phosphorylated Stat5 in breast cancer predicts poor clinical outcome and increased risk of antiestrogen therapy failure. Tyrosine 30-38 signal transducer and activator of transcription 5A Homo sapiens 54-59 20952588-6 2010 Enhanced and sustained prolactin-induced Stat5 tyrosine phosphorylation was observed in T47D and MCF7 breast cancer cells selectively in response to PTP1B depletion. Tyrosine 47-55 signal transducer and activator of transcription 5A Homo sapiens 41-46 21220747-4 2011 The STAT5-mediated protection requires tyrosine phosphorylation of STAT5 independent of JAK2 and transcriptional activity. Tyrosine 39-47 signal transducer and activator of transcription 5A Homo sapiens 4-9 21220747-4 2011 The STAT5-mediated protection requires tyrosine phosphorylation of STAT5 independent of JAK2 and transcriptional activity. Tyrosine 39-47 signal transducer and activator of transcription 5A Homo sapiens 67-72 21233313-6 2011 Pimozide decreases STAT5 tyrosine phosphorylation, although it does not inhibit BCR/ABL or other tyrosine kinases. Tyrosine 25-33 signal transducer and activator of transcription 5A Homo sapiens 19-24 21368206-4 2011 Association of RBP-retinol with STRA6 triggers tyrosine phosphorylation, resulting in recruitment and activation of JAK2 and the transcription factor STAT5. Tyrosine 47-55 signal transducer and activator of transcription 5A Homo sapiens 150-155 20952588-7 2010 Conversely, PTP1B overexpression suppressed prolactin-induced Stat5 tyrosine phosphorylation. Tyrosine 68-76 signal transducer and activator of transcription 5A Homo sapiens 62-67 20870009-11 2010 Significant phosphorylation of STAT-5 (tyr(694)) was observed at 12h. Tyrosine 39-42 signal transducer and activator of transcription 5A Homo sapiens 31-37 19923221-3 2010 Using phosphoamino acid analysis, JAK3 and STAT5 were determined to be serine and tyrosine-phosphorylated in response to IL-2 stimulation of the human natural killer-like cell line, YT. Tyrosine 82-90 signal transducer and activator of transcription 5A Homo sapiens 43-48 20372794-10 2010 Downstream STAT5 activity is also inhibited as shown by reduced STAT5 tyrosine phosphorylation and in vitro DNA binding. Tyrosine 70-78 signal transducer and activator of transcription 5A Homo sapiens 11-16 20372794-10 2010 Downstream STAT5 activity is also inhibited as shown by reduced STAT5 tyrosine phosphorylation and in vitro DNA binding. Tyrosine 70-78 signal transducer and activator of transcription 5A Homo sapiens 64-69 20495002-7 2010 37Beta ic is tyrosine-phosphorylated and associates with STAT-5, a canonic signal transducer of IL-2R. Tyrosine 13-21 signal transducer and activator of transcription 5A Homo sapiens 57-63 20127673-5 2010 TSLP induced the tyrosine-phosphorylation of STAT5 and the proliferation of multilineage-commited progenitor cells, pro-B cells and pre-B cells. Tyrosine 17-25 signal transducer and activator of transcription 5A Homo sapiens 45-50 19923221-7 2010 Moreover, inhibition of PP2A, but not PP1, diminished IL-2-induced tyrosine phosphorylation of IL-2Rbeta, JAK3, and STAT5, and abolished STAT5 DNA binding activity. Tyrosine 67-75 signal transducer and activator of transcription 5A Homo sapiens 116-121 19100521-7 2009 Tyrosines 579/581 played a critical role in mediating signals via the Ras/ERK and STAT5 pathways, with dual targeting of the tyrosine kinase activity of Tel/PDGFRbeta and the MEK/ERK pathway completely preventing Tel/PDGFRbeta-induced differentiation. Tyrosine 0-9 signal transducer and activator of transcription 5A Homo sapiens 82-87 19273609-6 2009 Together, our results define a novel negative cross-regulation between PRL and EGF involving the Jak2/Stat5a and Ras/MAPK pathways through tyrosine phosphorylation of Grb2. Tyrosine 139-147 signal transducer and activator of transcription 5A Homo sapiens 102-108 20196786-3 2009 SHP1 expression correlates with down-regulation of Janus kinase/signal transducers and activators of transcription (JAK2/STAT3/STAT5) signalling, which is mediated in part by tyrosine dephosphorylation events and modulation of the proteasome pathway. Tyrosine 175-183 signal transducer and activator of transcription 5A Homo sapiens 127-132 18996136-10 2009 STAT5 is phosphorylated in the cytoplasm at an activating tyrosine in response to E2 or EGF, and then is translocated to the nucleus to stimulate target gene transcription. Tyrosine 58-66 signal transducer and activator of transcription 5A Homo sapiens 0-5 19347282-4 2009 The activation of STATs depends on phosphorylation on a single tyrosine residue (e.g., Tyr705 in STAT3 and Tyr694 in STAT5) in the C-terminal domain. Tyrosine 63-71 signal transducer and activator of transcription 5A Homo sapiens 117-122 19136996-6 2008 The role for Tyr(1077), the major regulator of signal transducer and activator of transcription-5 (STAT5) during leptin signaling, in the physiologic response to leptin remains unclear, although the obese phenotype of animals deleted for STAT5 in the brain suggests the potential importance of this signaling pathway. Tyrosine 13-16 signal transducer and activator of transcription 5A Homo sapiens 99-104 18628457-6 2008 RESULT: The substitution of the tyrosine residues by phenylalanine in the juxtamembrane, interkinase, and COOH-terminal domains resulted in a complete loss of the transforming potential of FLT3-D835Y-expressing cells which can be attributed to a significant reduction of signal tranducer and activator of transcription 5 (STAT5) phosphorylation at the molecular level. Tyrosine 32-40 signal transducer and activator of transcription 5A Homo sapiens 271-320 18550772-4 2008 E2 treatment stimulated STAT5b tyrosine phosphorylation at the activating tyrosine Y699, resulting in increased STAT5-mediated transcriptional activity, which was inhibited by a Y669F STAT5b mutant. Tyrosine 31-39 signal transducer and activator of transcription 5A Homo sapiens 24-29 18550772-4 2008 E2 treatment stimulated STAT5b tyrosine phosphorylation at the activating tyrosine Y699, resulting in increased STAT5-mediated transcriptional activity, which was inhibited by a Y669F STAT5b mutant. Tyrosine 74-82 signal transducer and activator of transcription 5A Homo sapiens 24-29 18550772-6 2008 Both E2-induced STAT5b tyrosine phosphorylation and STAT5-mediated transcription were also inhibited by the ER antagonist ICI 182,780 and the c-Src inhibitor PP2, indicating additional requirements for the ER and c-Src kinase activity. Tyrosine 23-31 signal transducer and activator of transcription 5A Homo sapiens 16-21 18628457-6 2008 RESULT: The substitution of the tyrosine residues by phenylalanine in the juxtamembrane, interkinase, and COOH-terminal domains resulted in a complete loss of the transforming potential of FLT3-D835Y-expressing cells which can be attributed to a significant reduction of signal tranducer and activator of transcription 5 (STAT5) phosphorylation at the molecular level. Tyrosine 32-40 signal transducer and activator of transcription 5A Homo sapiens 322-327 18197699-4 2008 Here we characterize STAT5 tyrosine phosphorylation and its interaction with LMW-PTP during early phorbol-12-myristate-13-acetate-induced megakaryocyte differentiation; these processes show clear dependence on STAT5 threonine phosphorylation. Tyrosine 27-35 signal transducer and activator of transcription 5A Homo sapiens 21-26 18063684-8 2008 During decidualization, STAT5 was phosphorylated on tyrosine 694, a well-known SRC phosphorylation site. Tyrosine 52-60 signal transducer and activator of transcription 5A Homo sapiens 24-29 18197699-4 2008 Here we characterize STAT5 tyrosine phosphorylation and its interaction with LMW-PTP during early phorbol-12-myristate-13-acetate-induced megakaryocyte differentiation; these processes show clear dependence on STAT5 threonine phosphorylation. Tyrosine 27-35 signal transducer and activator of transcription 5A Homo sapiens 210-215 17846080-2 2008 Cytokines such as growth hormone, prolactin, erythropoietin, and interleukin-2 stimulate the activation of STAT5a by tyrosine phosphorylation. Tyrosine 117-125 signal transducer and activator of transcription 5A Homo sapiens 107-113 17846080-5 2008 This study explores the nuclear trafficking of STAT5a both before phosphorylation and after tyrosine phosphorylation. Tyrosine 92-100 signal transducer and activator of transcription 5A Homo sapiens 47-53 17846080-7 2008 Evaluation of a series of mutations and deletions identifies a region within the coiled coil domain of STAT5a that is critical for nuclear import of both unphosphorylated and tyrosine-phosphorylated forms. Tyrosine 175-183 signal transducer and activator of transcription 5A Homo sapiens 103-109 17846080-9 2008 However, after tyrosine phosphorylation, STAT5a accumulates in the nucleus because of its retention by DNA binding. Tyrosine 15-23 signal transducer and activator of transcription 5A Homo sapiens 41-47 17890450-3 2008 Stat5 has been implicated in malignant transformation, and moreover, the activated tyrosine phosphorylated form of Stat5 is frequently observed in human lymphomas. Tyrosine 83-91 signal transducer and activator of transcription 5A Homo sapiens 0-5 17890450-3 2008 Stat5 has been implicated in malignant transformation, and moreover, the activated tyrosine phosphorylated form of Stat5 is frequently observed in human lymphomas. Tyrosine 83-91 signal transducer and activator of transcription 5A Homo sapiens 115-120 17609438-4 2007 As expected, GH treatment increased JAK-dependent STAT5 tyrosine phosphorylation, which bound to the proximal STAT response element (pSRE) on the SOCS3 promoter. Tyrosine 56-64 signal transducer and activator of transcription 5A Homo sapiens 50-55 17503465-0 2007 Tyrosine-phosphorylated STAT5 accumulates on podosomes in Hck-transformed fibroblasts and chronic myeloid leukemia cells. Tyrosine 0-8 signal transducer and activator of transcription 5A Homo sapiens 24-29 17503465-4 2007 We demonstrated that in these cells, the tyrosine-phosphorylated form of STAT5 (PY-STAT5) increased and preferentially localized on podosomes together with Hck, instead of translocating to the nucleus as observed with conventional stimuli such as IFNgamma. Tyrosine 41-49 signal transducer and activator of transcription 5A Homo sapiens 73-78 17503465-4 2007 We demonstrated that in these cells, the tyrosine-phosphorylated form of STAT5 (PY-STAT5) increased and preferentially localized on podosomes together with Hck, instead of translocating to the nucleus as observed with conventional stimuli such as IFNgamma. Tyrosine 41-49 signal transducer and activator of transcription 5A Homo sapiens 80-88 17764157-2 2007 While Crk L is a bona fide nuclear signaling protein because of its ability to bind tyrosine-phosphorylated STAT5 and act as a transcriptional coactivator, the function of nuclear Crk II is less well understood. Tyrosine 84-92 signal transducer and activator of transcription 5A Homo sapiens 108-113 16757551-7 2006 Tyrosine-phosphorylated STAT5 was detected in muscle and fat of all subjects after GH. Tyrosine 0-8 signal transducer and activator of transcription 5A Homo sapiens 24-29 17505063-6 2007 s80HER4 was constitutively tyrosine phosphorylated, and treatment of cells with a specific HER family tyrosine kinase inhibitor 1) blocked tyrosine phosphorylation; 2) markedly diminished GFP-s80HER4 nuclear localization; and 3) reduced signal transducer and activator of transcription (STAT)5A tyrosine phosphorylation and nuclear localization as well as GFP-s80HER4:STAT5A interaction. Tyrosine 102-110 signal transducer and activator of transcription 5A Homo sapiens 237-294 17505063-6 2007 s80HER4 was constitutively tyrosine phosphorylated, and treatment of cells with a specific HER family tyrosine kinase inhibitor 1) blocked tyrosine phosphorylation; 2) markedly diminished GFP-s80HER4 nuclear localization; and 3) reduced signal transducer and activator of transcription (STAT)5A tyrosine phosphorylation and nuclear localization as well as GFP-s80HER4:STAT5A interaction. Tyrosine 102-110 signal transducer and activator of transcription 5A Homo sapiens 368-374 17505063-6 2007 s80HER4 was constitutively tyrosine phosphorylated, and treatment of cells with a specific HER family tyrosine kinase inhibitor 1) blocked tyrosine phosphorylation; 2) markedly diminished GFP-s80HER4 nuclear localization; and 3) reduced signal transducer and activator of transcription (STAT)5A tyrosine phosphorylation and nuclear localization as well as GFP-s80HER4:STAT5A interaction. Tyrosine 102-110 signal transducer and activator of transcription 5A Homo sapiens 237-294 17505063-6 2007 s80HER4 was constitutively tyrosine phosphorylated, and treatment of cells with a specific HER family tyrosine kinase inhibitor 1) blocked tyrosine phosphorylation; 2) markedly diminished GFP-s80HER4 nuclear localization; and 3) reduced signal transducer and activator of transcription (STAT)5A tyrosine phosphorylation and nuclear localization as well as GFP-s80HER4:STAT5A interaction. Tyrosine 102-110 signal transducer and activator of transcription 5A Homo sapiens 368-374 17161871-3 2007 Results showed that CB-SC could significantly inhibit lymphocyte proliferation and reduce tyrosine phosphorylation of STAT5 in both PHA- and IL-2-stimulated lymphocytes, along with the regulation on the phenotypes of CD4+ and CD8+ T cells. Tyrosine 90-98 signal transducer and activator of transcription 5A Homo sapiens 118-123 17785772-0 2007 Cutting edge: selective tyrosine dephosphorylation of interferon-activated nuclear STAT5 by the VHR phosphatase. Tyrosine 24-32 signal transducer and activator of transcription 5A Homo sapiens 83-88 17785772-1 2007 Cytokine-induced tyrosine phosphorylation of the transcription factor STAT5 is required for its transcriptional activity. Tyrosine 17-25 signal transducer and activator of transcription 5A Homo sapiens 70-75 17785772-2 2007 In this article we show that the small dual-specificity phosphatase VHR selectively dephosphorylates IFN-alpha- and beta-activated, tyrosine-phosphorylated STAT5, leading to the subsequent inhibition of STAT5 function. Tyrosine 132-140 signal transducer and activator of transcription 5A Homo sapiens 156-161 17785772-2 2007 In this article we show that the small dual-specificity phosphatase VHR selectively dephosphorylates IFN-alpha- and beta-activated, tyrosine-phosphorylated STAT5, leading to the subsequent inhibition of STAT5 function. Tyrosine 132-140 signal transducer and activator of transcription 5A Homo sapiens 203-208 17785772-3 2007 Phosphorylation of VHR at Tyr(138) was required for its phosphatase activity toward STAT5. Tyrosine 26-29 signal transducer and activator of transcription 5A Homo sapiens 84-89 17785772-5 2007 The tyrosine kinase Tyk2, which mediates the phosphorylation of STAT5, was also responsible for the phosphorylation of VHR at Tyr(138). Tyrosine 126-129 signal transducer and activator of transcription 5A Homo sapiens 64-69 16650892-0 2006 Src transduces signaling via growth hormone (GH)-activated GH receptor (GHR) tyrosine-phosphorylating GHR and STAT5 in human leukemia cells. Tyrosine 77-85 signal transducer and activator of transcription 5A Homo sapiens 110-115 16650892-3 2006 The tyrosine phosphorylation levels of GHR and STAT5 induced by GH decreased in the presence of PP2 Src kinase inhibitor. Tyrosine 4-12 signal transducer and activator of transcription 5A Homo sapiens 47-52 16650892-5 2006 The treatment of F-36P cells with the antisense src oligonucleotides, which selectively decreased the Src expression, reduced the rhGH-induced tyrosine phosphorylation of the STAT5 activation sites. Tyrosine 143-151 signal transducer and activator of transcription 5A Homo sapiens 175-180 16950496-1 2006 The growth hormone (GH) receptor (R)-mediated JAK2 (Janus kinase-2)-STAT5 (signaling transducer and activator of transcription-5) pathway involves a cascade of protein-protein interactions and tyrosine phosphorylations that occur in a spatially and temporally sensitive manner in cells. Tyrosine 193-201 signal transducer and activator of transcription 5A Homo sapiens 68-73 16532027-1 2006 Signal transducers and activator of transcription 5 (STAT5) A and B are transcriptional regulators that play a central role in cytokine signaling in the hematopoietic lineage and which are frequently activated in a persistent manner in human leukemia/lymphoma, as assessed by their constitutive tyrosine phosphorylation and DNA-binding activity. Tyrosine 295-303 signal transducer and activator of transcription 5A Homo sapiens 0-51 16532027-1 2006 Signal transducers and activator of transcription 5 (STAT5) A and B are transcriptional regulators that play a central role in cytokine signaling in the hematopoietic lineage and which are frequently activated in a persistent manner in human leukemia/lymphoma, as assessed by their constitutive tyrosine phosphorylation and DNA-binding activity. Tyrosine 295-303 signal transducer and activator of transcription 5A Homo sapiens 53-58 16568084-9 2006 B cell receptor engagement in SLP65-deficient B cell lymphoma cells also resulted in tyrosine-phosphorylation of the proliferation- and survival-related MAPK1 and STAT5 molecules, which was sensitive to silencing of the SRC kinase LYN. Tyrosine 85-93 signal transducer and activator of transcription 5A Homo sapiens 163-168 16407271-5 2006 Selective mutation of the eight tyrosine residues of the EpoR cytoplasmic domain results in impaired or absent activation of either STAT5 (mutation of Tyr(343)) or AKT (mutation of Tyr(479)) or both (mutation of all eight tyrosine residues). Tyrosine 32-40 signal transducer and activator of transcription 5A Homo sapiens 132-137 16527988-3 2006 Thrombin activated STAT-5 as measured by its tyrosine phosphorylation, DNA binding, and reporter gene activity. Tyrosine 45-53 signal transducer and activator of transcription 5A Homo sapiens 19-25 16569768-1 2006 A broad spectrum of cytokines can activate the signal transducer and activator of transcription 5 (Stat5) by inducing a single tyrosine phosphorylation of the molecule. Tyrosine 127-135 signal transducer and activator of transcription 5A Homo sapiens 47-97 16569768-1 2006 A broad spectrum of cytokines can activate the signal transducer and activator of transcription 5 (Stat5) by inducing a single tyrosine phosphorylation of the molecule. Tyrosine 127-135 signal transducer and activator of transcription 5A Homo sapiens 99-104 16529541-3 2006 STAT3 and STAT5 acquire oncogenic potential through constitutive phosphorylation on tyrosine, and their activity has been shown to be required to sustain a transformed phenotype. Tyrosine 84-92 signal transducer and activator of transcription 5A Homo sapiens 10-15 15976008-6 2005 Introduction of ERalpha and treatment with E2 decreased EGF-induced tyrosine phosphorylation of STAT5b, basal and EGF-induced STAT5-mediated transcription, and EGF-stimulated DNA synthesis in these cells. Tyrosine 68-76 signal transducer and activator of transcription 5A Homo sapiens 96-101 16765629-3 2006 Our results demonstrate that stimulation of MCF7 cells with epidermal growth factor (EGF) promoted an increase in the phosphorylation of STAT5 at Tyr-694, as revealed by site-specific antibodies that recognized the phosphorylated state of this residue. Tyrosine 146-149 signal transducer and activator of transcription 5A Homo sapiens 137-142 16765629-6 2006 However, STAT5 phosphorylation at Tyr-694 was dependent on the integrity of microtubule network and it was independent of the integrity of actin cytoskeleton. Tyrosine 34-37 signal transducer and activator of transcription 5A Homo sapiens 9-14 16568834-10 2006 A correlation between alteration in tyrosine phosphorylation level of STAT5 and the expression of the high affinity IL-5 receptor was established. Tyrosine 36-44 signal transducer and activator of transcription 5A Homo sapiens 70-75 16033816-9 2005 These data indicate that Tyr 729 of the G-CSF-R is required for SOCS1- and SOCS3-mediated negative regulation of G-CSF-R signaling and that the duration and intensity of G-CSF-induced Stat5 activation are regulated by two distinct mechanisms. Tyrosine 25-28 signal transducer and activator of transcription 5A Homo sapiens 184-189 16139998-2 2005 Stimulation of MCF7 cells with type IV collagen (Col-IV) promoted a striking increase in the phosphorylation of STAT5 at Tyr-694, as revealed by site-specific antibodies that recognized the phosphorylated state of this residue. Tyrosine 121-124 signal transducer and activator of transcription 5A Homo sapiens 112-117 16139998-4 2005 Treatment with the selective Src family inhibitor pyrazolopyrimidine PP-2 prevented STAT5 phosphorylation at Tyr-694, nuclear translocation of STAT5 and the STAT5-DNA complex formation. Tyrosine 109-112 signal transducer and activator of transcription 5A Homo sapiens 84-89 16139998-5 2005 Our results demonstrate, for the first time, that stimulation with Col-IV induces STAT5 phosphorylation of endogenous STAT5 at Tyr-694, nuclear translocation of STAT5 and increases in STAT5 DNA binding activity via a Src-dependent pathway in MCF7 cells. Tyrosine 127-130 signal transducer and activator of transcription 5A Homo sapiens 82-87 16139998-5 2005 Our results demonstrate, for the first time, that stimulation with Col-IV induces STAT5 phosphorylation of endogenous STAT5 at Tyr-694, nuclear translocation of STAT5 and increases in STAT5 DNA binding activity via a Src-dependent pathway in MCF7 cells. Tyrosine 127-130 signal transducer and activator of transcription 5A Homo sapiens 118-123 16139998-5 2005 Our results demonstrate, for the first time, that stimulation with Col-IV induces STAT5 phosphorylation of endogenous STAT5 at Tyr-694, nuclear translocation of STAT5 and increases in STAT5 DNA binding activity via a Src-dependent pathway in MCF7 cells. Tyrosine 127-130 signal transducer and activator of transcription 5A Homo sapiens 118-123 16139998-5 2005 Our results demonstrate, for the first time, that stimulation with Col-IV induces STAT5 phosphorylation of endogenous STAT5 at Tyr-694, nuclear translocation of STAT5 and increases in STAT5 DNA binding activity via a Src-dependent pathway in MCF7 cells. Tyrosine 127-130 signal transducer and activator of transcription 5A Homo sapiens 118-123 15727811-3 2005 Cytokine receptor-associated Janus kinases (JAKs) induce dimerization, nuclear translocation, and DNA binding through tyrosine phosphorylation of STAT5. Tyrosine 118-126 signal transducer and activator of transcription 5A Homo sapiens 146-151 15863494-2 2005 ERBB4 activities in the breast are mediated through the signal transducer and activator of transcription (STAT) family member STAT5A, and ERBB4 directly activates STAT5A, in part, through phosphorylation of STAT5A at the regulatory Tyr-694. Tyrosine 232-235 signal transducer and activator of transcription 5A Homo sapiens 126-132 15863494-2 2005 ERBB4 activities in the breast are mediated through the signal transducer and activator of transcription (STAT) family member STAT5A, and ERBB4 directly activates STAT5A, in part, through phosphorylation of STAT5A at the regulatory Tyr-694. Tyrosine 232-235 signal transducer and activator of transcription 5A Homo sapiens 163-169 15863494-2 2005 ERBB4 activities in the breast are mediated through the signal transducer and activator of transcription (STAT) family member STAT5A, and ERBB4 directly activates STAT5A, in part, through phosphorylation of STAT5A at the regulatory Tyr-694. Tyrosine 232-235 signal transducer and activator of transcription 5A Homo sapiens 163-169 15878737-8 2005 In the context of EPOR-ME, Y344 was required for Epo-induced Stat5 tyrosine phosphorylation. Tyrosine 67-75 signal transducer and activator of transcription 5A Homo sapiens 61-66 15671147-4 2005 This costimulatory effect of SCF was reflected in an increase in TPO-induced STAT5 DNA binding and increased and prolonged STAT5 tyrosine phosphorylation in both MO7e cells and primary human megakaryocyte progenitors. Tyrosine 129-137 signal transducer and activator of transcription 5A Homo sapiens 123-128 15671147-8 2005 In addition, the Src kinase inhibitor SU6656 partially downregulated the additional effect of SCF costimulation on STAT5 tyrosine phosphorylation. Tyrosine 121-129 signal transducer and activator of transcription 5A Homo sapiens 115-120 15671147-9 2005 SCF-induced enhancement of JAK2 phosphorylation was not affected by inhibition of Src kinase, suggesting that both JAK2 and Src kinase mediate STAT5 tyrosine phosphorylation. Tyrosine 149-157 signal transducer and activator of transcription 5A Homo sapiens 143-148 15345593-6 2005 Primary AML blasts bearing FLT3-Y842C mutations showed constitutive FLT3 and signal transducer and activator of transcription 5 (STAT-5) tyrosine phosphorylation. Tyrosine 137-145 signal transducer and activator of transcription 5A Homo sapiens 77-127 15345593-6 2005 Primary AML blasts bearing FLT3-Y842C mutations showed constitutive FLT3 and signal transducer and activator of transcription 5 (STAT-5) tyrosine phosphorylation. Tyrosine 137-145 signal transducer and activator of transcription 5A Homo sapiens 129-135 15536163-8 2005 This effect might result from interference of C-terminal nonsense sequence in mutated GHR with STAT5 docking to upstream tyrosine residues. Tyrosine 121-129 signal transducer and activator of transcription 5A Homo sapiens 95-100 16155412-5 2005 Imunoprecipitation and subsequent Western analysis showed that Jak2 leads to the tyrosine phosphorylation and activation of STAT5a or STAT5b under hypoxic conditions. Tyrosine 81-89 signal transducer and activator of transcription 5A Homo sapiens 124-130 15863494-7 2005 STAT5A Ser-127/Ser-128, on the other hand, was required for ERBB4-induced phosphorylation of Tyr-694, whereas Ser-779 and as yet unidentified tyrosine residues were phosphorylated in the absence of Ser-127/Ser-128. Tyrosine 93-96 signal transducer and activator of transcription 5A Homo sapiens 0-6 15863494-7 2005 STAT5A Ser-127/Ser-128, on the other hand, was required for ERBB4-induced phosphorylation of Tyr-694, whereas Ser-779 and as yet unidentified tyrosine residues were phosphorylated in the absence of Ser-127/Ser-128. Tyrosine 142-150 signal transducer and activator of transcription 5A Homo sapiens 0-6 15927792-4 2005 After GM-CSF stimulation, repressor STAT5A and B isoforms (80-77kDa) in autoimmune human and NOD monocytes and activator STAT5A (96-94kDa) and B (94-92kDa) isoforms in NOD macrophages stay persistently tyrosine phosphorylated. Tyrosine 202-210 signal transducer and activator of transcription 5A Homo sapiens 36-48 15702476-5 2005 In most cytokine responsive cell systems, STAT5 function is modulated by JAK2-dependent tyrosine phosphorylation. Tyrosine 88-96 signal transducer and activator of transcription 5A Homo sapiens 42-47 15711548-2 2005 Here we describe a previously unknown subpopulation of B cells in the human germinal center that is characterized by tyrosine phosphorylated transcriptional activator STAT5. Tyrosine 117-125 signal transducer and activator of transcription 5A Homo sapiens 167-172 15609129-3 2004 Latent cytoplasmic Stat5a is activated by tyrosine phosphorylation and following dimerization undergoes nuclear import. Tyrosine 42-50 signal transducer and activator of transcription 5A Homo sapiens 19-25 15356145-8 2004 In contrast, STAT5 activation was strongly enhanced by Gab2 tyrosine 614F, slightly reduced by Gab2 WT and strongly inhibited by Gab2 serine 623A. Tyrosine 60-68 signal transducer and activator of transcription 5A Homo sapiens 13-18 15194700-7 2004 We identify tyrosine residues 652 and 721 in the cytoplasmic region of the longest isoform of GPL (GPL(745)) as the major STAT5- and STAT3-activating sites, respectively. Tyrosine 12-20 signal transducer and activator of transcription 5A Homo sapiens 122-127 15242752-7 2004 Moreover, different EDCs significantly decreased the tyrosine phosphorylation state of STAT3 and STAT5, showing a distinct effect with respect to E(2). Tyrosine 53-61 signal transducer and activator of transcription 5A Homo sapiens 97-102 15001470-3 2004 Signal transduction studies revealed that JTZ-132 induced tyrosine phosphorylation of c-Mpl, Janus kinase-2 (JAK2), and signal transducers and activators of transcription 5 (STAT5) in UT-7/TPO cells as well as TPO. Tyrosine 58-66 signal transducer and activator of transcription 5A Homo sapiens 174-179 15166220-2 2004 Their receptor is composed of two chains, interleukin-28R/likely interleukin or cytokine or receptor 2 (IL-28R/LICR2) and IL-10R beta, and mediates the tyrosine phosphorylation of STAT1, STAT2, STAT3, and STAT5. Tyrosine 152-160 signal transducer and activator of transcription 5A Homo sapiens 205-210 15169792-3 2004 MATERIALS AND METHODS: Immunohistochemistry was used to detect active, tyrosine-phosphorylated Stat5 in paraffin-embedded breast cancer specimens from three archival tissue microarray materials A, B, and C. Material A included 19 healthy human breast tissues and a progression series of primary lymph node-negative, primary lymph node-positive, and metastatic breast cancer (n = 400). Tyrosine 71-79 signal transducer and activator of transcription 5A Homo sapiens 95-100 15010456-8 2004 Correspondingly, GH-induced tyrosine-phosphorylated GHR, JAK2, and ERK1/2 were highly represented in the CM fraction, whereas tyrosine-phosphorylated STAT5 was enriched in the non-membranous fraction of the post-nuclear supernatant. Tyrosine 126-134 signal transducer and activator of transcription 5A Homo sapiens 150-155 14696092-0 2004 Stat5a is tyrosine phosphorylated and nuclear localized in a high proportion of human breast cancers. Tyrosine 10-18 signal transducer and activator of transcription 5A Homo sapiens 0-6 14696092-5 2004 Immunoprecipitation/Western blotting and immunohistochemistry assays employing different phospho-specific antibodies verified Stat5a tyrosine phosphorylation status. Tyrosine 133-141 signal transducer and activator of transcription 5A Homo sapiens 126-132 14741732-6 2004 Western blot analysis showed that tumor supernatants suppressed expression of JAK3 and tyrosine phosphorylation of STAT5. Tyrosine 87-95 signal transducer and activator of transcription 5A Homo sapiens 115-120 14730712-6 2004 Stable transfection of EOC-20 cells with a dominant negative JAK2 mutant also blocked IL-3-induced tyrosine phosphorylation of JAK2, STAT5A, and STAT5B in microglia. Tyrosine 99-107 signal transducer and activator of transcription 5A Homo sapiens 133-139 15052767-5 2004 RESULTS: Tyrosine phosphorylation of STAT-3 and STAT-5 was undetectable in unstimulated CD34+ cells, but was evident at 15 min in response to VEGF stimulation. Tyrosine 9-17 signal transducer and activator of transcription 5A Homo sapiens 48-54 12764361-0 2003 In BCR-ABL-positive cells, STAT-5 tyrosine-phosphorylation integrates signals induced by imatinib mesylate and Ara-C. Tyrosine 34-42 signal transducer and activator of transcription 5A Homo sapiens 27-33 12909618-0 2003 Roles for an Epo receptor Tyr-343 Stat5 pathway in proliferative co-signaling with kit. Tyrosine 26-29 signal transducer and activator of transcription 5A Homo sapiens 34-39 12879454-0 2003 Immunoreactivity of Stat5 phosphorylated on tyrosine as a cell-based measure of Bcr/Abl kinase activity. Tyrosine 44-52 signal transducer and activator of transcription 5A Homo sapiens 20-25 12719422-3 2003 Specifically, Stat5 was activated in 65% of human prostate cancer specimens examined based on nuclear location of tyrosine phosphorylated Stat5. Tyrosine 114-122 signal transducer and activator of transcription 5A Homo sapiens 14-19 14551225-7 2004 The D351 form normally has the GH-induced JAK/STAT5 tyrosine phosphorylation. Tyrosine 52-60 signal transducer and activator of transcription 5A Homo sapiens 46-51 12796379-10 2003 Moreover, the constitutive tyrosine phosphorylation of STAT5 was efficiently down-regulated by a FLT3 protein tyrosine kinase inhibitor in AML cells expressing an active FLT3 mutant. Tyrosine 27-35 signal transducer and activator of transcription 5A Homo sapiens 55-60 12829027-2 2003 METHODS: STAT5 activation was examined in erythroid cell lines by analyzing the effects of EPO and SCF on STAT5 tyrosine phosphorylation, serine phosphorylation, DNA binding, and STAT5-mediated gene transactivation. Tyrosine 112-120 signal transducer and activator of transcription 5A Homo sapiens 9-14 12764361-1 2003 In BCR-ABL-positive cells, the transcription factor STAT-5 is constitutively activated by tyrosine phosphorylation. Tyrosine 90-98 signal transducer and activator of transcription 5A Homo sapiens 52-58 12764361-4 2003 Imatinib mesylate treatment strongly suppressed STAT-5 tyrosine-phosphorylation in K562 and primary CML blasts. Tyrosine 55-63 signal transducer and activator of transcription 5A Homo sapiens 48-54 12764361-8 2003 Exposure to Ara-C resulted in significant downregulation of STAT-5 tyrosine-phosphorylation and inhibition of DNA binding. Tyrosine 67-75 signal transducer and activator of transcription 5A Homo sapiens 60-66 12764361-10 2003 Overall, in this report we demonstrate that STAT-5 tyrosine-phosphorylation is a specific target of imatinib mesylate and Ara-C. Tyrosine 51-59 signal transducer and activator of transcription 5A Homo sapiens 44-50 12764361-11 2003 Our results suggest that, in combination therapy, inhibition of STAT-5 tyrosine-phosphorylation may be responsible for synergistic or additive effects on BCR-ABL-positive cells. Tyrosine 71-79 signal transducer and activator of transcription 5A Homo sapiens 64-70 12204892-4 2002 NRG-1 induced a rapid and transient increase in tyrosine phosphorylation of TYK2 and JAK3, but not JAK1 or JAK2, and induced STAT3 and STAT5 tyrosine phosphorylation. Tyrosine 141-149 signal transducer and activator of transcription 5A Homo sapiens 135-140 12621061-8 2003 Furthermore, this mutation inhibited v-Src-induced cyclin D1-luciferase reporter activity in transient transfection assays performed in Stat5a/b-deficient MEFs, suggesting that Tyr-679 phosphorylation may play a role in v-Src induced proliferation. Tyrosine 177-180 signal transducer and activator of transcription 5A Homo sapiens 136-142 15687690-7 2003 Both tyrosine and serine phosphorylation regulate Stat5 activity, as does the interaction of this transcription factor with co-activators and -repressors within the nucleus. Tyrosine 5-13 signal transducer and activator of transcription 5A Homo sapiens 50-55 12529425-4 2003 RhoA triggers tyrosine phosphorylation (Y696) of Stat5a via a JAK2-dependent mechanism and promotes DNA-binding activity of Stat5a. Tyrosine 14-22 signal transducer and activator of transcription 5A Homo sapiens 49-55 12529425-4 2003 RhoA triggers tyrosine phosphorylation (Y696) of Stat5a via a JAK2-dependent mechanism and promotes DNA-binding activity of Stat5a. Tyrosine 14-22 signal transducer and activator of transcription 5A Homo sapiens 124-130 12529425-5 2003 Tyrosine phosphorylation of Stat5a is also stimulated physiologically by lysophosphatidic acid (LPA) in a Rho-dependent manner. Tyrosine 0-8 signal transducer and activator of transcription 5A Homo sapiens 28-34 12529425-8 2003 Thus, RhoA regulates Stat5a via tyrosine phosphorylation and via a yet to be determined novel down-modulating pathway that involves serine dephosphorylation. Tyrosine 32-40 signal transducer and activator of transcription 5A Homo sapiens 21-27 12384143-0 2002 Transforming growth factor-beta(1) augments granulocyte-macrophage colony-stimulating factor-induced proliferation of umbilical cord blood CD34(+) cells with an associated tyrosine phosphorylation of STAT5. Tyrosine 172-180 signal transducer and activator of transcription 5A Homo sapiens 200-205 12384143-7 2002 Tyrosine phosphorylation of STAT5 induced by GM-CSF was enhanced by stimulation with the combination of TGF-beta(1) and GM-CSF (TGF-beta(1)/GM-CSF) compared with that induced by GM-CSF alone in CD34(+) cells and FKH-1 cells. Tyrosine 0-8 signal transducer and activator of transcription 5A Homo sapiens 28-33 12384143-10 2002 CONCLUSIONS: Results suggest that TGF-beta(1) may augment GM-CSF-induced proliferation of CD34(+) cells in association with enhanced tyrosine phosphorylation of STAT5. Tyrosine 133-141 signal transducer and activator of transcription 5A Homo sapiens 161-166 12615921-1 2003 Stat5A, a member of the signal transducers and activators of transcription (Stat) family, is activated upon a single tyrosine phosphorylation. Tyrosine 117-125 signal transducer and activator of transcription 5A Homo sapiens 0-6 12615921-2 2003 Although much is known about the activation process, the mechanism by which the tyrosine-phosphorylated Stat5A proteins are inactivated is largely unknown. Tyrosine 80-88 signal transducer and activator of transcription 5A Homo sapiens 104-110 12615921-3 2003 In this report, we demonstrate that down-regulation of the tyrosine-phosphorylated Stat5A was via dephosphorylation. Tyrosine 59-67 signal transducer and activator of transcription 5A Homo sapiens 83-89 12615921-4 2003 Using tyrosine-phosphorylated peptides derived from Stat5A, we were able to purify protein-tyrosine phosphatase Shp-2 from cell lysates. Tyrosine 6-14 signal transducer and activator of transcription 5A Homo sapiens 52-58 12615921-5 2003 Shp-2, but not Shp-1, specifically interacted with Stat5A in vivo, and the interaction was tyrosine phosphorylation-dependent. Tyrosine 91-99 signal transducer and activator of transcription 5A Homo sapiens 51-57 12642867-8 2003 Stat5aDelta740 was selected for adenoviral delivery, and high-efficiency expression of tyrosine phosphorylated Stat5aDelta740 was achieved in infected cells. Tyrosine 87-95 signal transducer and activator of transcription 5A Homo sapiens 0-14 12642867-8 2003 Stat5aDelta740 was selected for adenoviral delivery, and high-efficiency expression of tyrosine phosphorylated Stat5aDelta740 was achieved in infected cells. Tyrosine 87-95 signal transducer and activator of transcription 5A Homo sapiens 111-125 12543077-5 2002 However, the constitutive tyrosine phosphorylation of Stat3 as well as IL-2 induced Stat5 tyrosine phosphorylation and DNA binding were unaffected by the stably transfected wild-type SHP2 as well as the inactive SHP2. Tyrosine 90-98 signal transducer and activator of transcription 5A Homo sapiens 84-89 12419827-10 2002 Moreover, reduced tyrosine phosphorylation of STAT5 in the experimental blocks was confirmed by western blotting analysis. Tyrosine 18-26 signal transducer and activator of transcription 5A Homo sapiens 46-51 12377952-2 2002 Progression of T cells through G(1)-S phase of cell cycle requires T cell receptor (TCR)- and/or cytokine-inducible tyrosine phosphorylation of Stat5a/b. Tyrosine 116-124 signal transducer and activator of transcription 5A Homo sapiens 144-150 12351686-3 2002 In endothelial cells, Stat5 and Stat3 are rapidly phosphorylated on both tyrosine and serine residues in response to 17beta-estradiol, and nuclear translocation is subsequently induced. Tyrosine 73-81 signal transducer and activator of transcription 5A Homo sapiens 22-27 12204892-6 2002 Inhibition of HER2"s ability to dimerize using the HER2-specific antibody 2C4 completely blocked NRG-1-induced JAK3, TYK2, STAT3, and STAT5 tyrosine phosphorylation. Tyrosine 140-148 signal transducer and activator of transcription 5A Homo sapiens 134-139 12198240-7 2002 Both the tyrosine phosphorylated and unphosphorylated forms of Stat5, as well as Stat5a/Stat5b heterodimers, could associate with CPAP. Tyrosine 9-17 signal transducer and activator of transcription 5A Homo sapiens 63-68 11751923-6 2002 Luciferase assays using a STAT5-specific DNA sequence demonstrate that, although Tyr-699 is absolutely required for transcriptional activation, tyrosines 725, 740, and 743 may be involved in a negative regulation of transcription. Tyrosine 81-84 signal transducer and activator of transcription 5A Homo sapiens 26-31 12060651-3 2002 In addition, overexpression studies indicate that the carboxyl-terminal SH2 domain of SHP-2 is required to maintain tyrosine phosphorylation of Stat5 and its interaction with SHP-2. Tyrosine 116-124 signal transducer and activator of transcription 5A Homo sapiens 144-149 12087074-6 2002 Within the vas deferens, PRL induced rapid tyrosine phosphorylation of JAK 2 and STAT 5 (after 10 and 20 min respectively), and tyrosine and threonine phosphorylation of ERK 1 and 2 (after 5 min). Tyrosine 43-51 signal transducer and activator of transcription 5A Homo sapiens 81-87 12089361-9 2002 The cooperation depends on the PRL-induced phosphorylation on Tyr(694) in Stat5A and Tyr(699) in Stat5B, as demonstrated using the Stat5AY694F and Stat5BY699F proteins. Tyrosine 62-65 signal transducer and activator of transcription 5A Homo sapiens 74-80 11875080-9 2002 STAT5A/B phosphorylation is downstream of EPO-R Tyr(343), however, STAT5A/B serine phosphorylation is unaffected by either ERK or p38 inhibition. Tyrosine 48-51 signal transducer and activator of transcription 5A Homo sapiens 0-6 11751923-6 2002 Luciferase assays using a STAT5-specific DNA sequence demonstrate that, although Tyr-699 is absolutely required for transcriptional activation, tyrosines 725, 740, and 743 may be involved in a negative regulation of transcription. Tyrosine 144-153 signal transducer and activator of transcription 5A Homo sapiens 26-31 11731617-1 2001 Signal transducer and activator of transcription 5b (STAT5b), the major liver-expressed STAT5 form, is phosphorylated on both tyrosine and serine in GH-stimulated cells. Tyrosine 126-134 signal transducer and activator of transcription 5A Homo sapiens 53-58 11577084-4 2001 In transfection assays both Stat5 isoforms, Stat5a and Stat5b, were phosphorylated on tyrosine in response to IFN-gamma. Tyrosine 86-94 signal transducer and activator of transcription 5A Homo sapiens 28-33 11577084-4 2001 In transfection assays both Stat5 isoforms, Stat5a and Stat5b, were phosphorylated on tyrosine in response to IFN-gamma. Tyrosine 86-94 signal transducer and activator of transcription 5A Homo sapiens 44-50 11577084-6 2001 Moreover, a peptide including Tyr-440, the Stat1 binding site of the human IFNGR1 chain, conferred the ability upon a synthetic receptor to activate Stat5. Tyrosine 30-33 signal transducer and activator of transcription 5A Homo sapiens 149-154 11753614-1 2001 In the present study, we examined the underlying mechanism, which causes the constitutive tyrosine phosphorylation of signal transducer and activator of transcription 5 (STAT5) in acute myeloid leukemia (AML) blasts. Tyrosine 90-98 signal transducer and activator of transcription 5A Homo sapiens 118-168 11753614-1 2001 In the present study, we examined the underlying mechanism, which causes the constitutive tyrosine phosphorylation of signal transducer and activator of transcription 5 (STAT5) in acute myeloid leukemia (AML) blasts. Tyrosine 90-98 signal transducer and activator of transcription 5A Homo sapiens 170-175 11641791-7 2001 Reduction of Src diminished tyrosine phosphorylation of STAT5 in K562 cells regardless of EPO treatment. Tyrosine 28-36 signal transducer and activator of transcription 5A Homo sapiens 56-61 11641791-8 2001 The tyrosine phosphorylation level of STAT5 induced by EPO in F-36P cells was reduced in the presence of PP1 or PP2 selective Src inhibitor. Tyrosine 4-12 signal transducer and activator of transcription 5A Homo sapiens 38-43 11641791-0 2001 Src directly tyrosine-phosphorylates STAT5 on its activation site and is involved in erythropoietin-induced signaling pathway. Tyrosine 13-21 signal transducer and activator of transcription 5A Homo sapiens 37-42 11641791-9 2001 In addition, the expression of dominant negative Src in F-36P cells reduced the tyrosine phosphorylation of STAT5. Tyrosine 80-88 signal transducer and activator of transcription 5A Homo sapiens 108-113 11641791-2 2001 In erythropoietin receptor (EPOR)-mediated signaling, STAT5 is tyrosine-phosphorylated by EPO stimulation. Tyrosine 63-71 signal transducer and activator of transcription 5A Homo sapiens 54-59 11641791-10 2001 When Src and STAT5 were co-expressed in COS7 cells, tyrosine phosphorylation of STAT5 was observed, and tyrosine residue 694 (Tyr 694) of STAT5A was identified as the major phosphorylation site by Src. Tyrosine 52-60 signal transducer and activator of transcription 5A Homo sapiens 13-18 11641791-10 2001 When Src and STAT5 were co-expressed in COS7 cells, tyrosine phosphorylation of STAT5 was observed, and tyrosine residue 694 (Tyr 694) of STAT5A was identified as the major phosphorylation site by Src. Tyrosine 52-60 signal transducer and activator of transcription 5A Homo sapiens 80-85 11641791-10 2001 When Src and STAT5 were co-expressed in COS7 cells, tyrosine phosphorylation of STAT5 was observed, and tyrosine residue 694 (Tyr 694) of STAT5A was identified as the major phosphorylation site by Src. Tyrosine 104-112 signal transducer and activator of transcription 5A Homo sapiens 13-18 11641791-10 2001 When Src and STAT5 were co-expressed in COS7 cells, tyrosine phosphorylation of STAT5 was observed, and tyrosine residue 694 (Tyr 694) of STAT5A was identified as the major phosphorylation site by Src. Tyrosine 104-112 signal transducer and activator of transcription 5A Homo sapiens 138-144 11641791-10 2001 When Src and STAT5 were co-expressed in COS7 cells, tyrosine phosphorylation of STAT5 was observed, and tyrosine residue 694 (Tyr 694) of STAT5A was identified as the major phosphorylation site by Src. Tyrosine 126-129 signal transducer and activator of transcription 5A Homo sapiens 13-18 11641791-10 2001 When Src and STAT5 were co-expressed in COS7 cells, tyrosine phosphorylation of STAT5 was observed, and tyrosine residue 694 (Tyr 694) of STAT5A was identified as the major phosphorylation site by Src. Tyrosine 126-129 signal transducer and activator of transcription 5A Homo sapiens 138-144 11641791-11 2001 In vitro kinase assay revealed that GST-STAT5 fusion protein with the conserved C-terminal, but not the C-terminal-truncated mutant which lacks Tyr 694, was tyrosine-phosphorylated by Src. Tyrosine 144-147 signal transducer and activator of transcription 5A Homo sapiens 40-45 11641791-11 2001 In vitro kinase assay revealed that GST-STAT5 fusion protein with the conserved C-terminal, but not the C-terminal-truncated mutant which lacks Tyr 694, was tyrosine-phosphorylated by Src. Tyrosine 157-165 signal transducer and activator of transcription 5A Homo sapiens 40-45 11641791-12 2001 Src can thus directly tyrosine-phosphorylate the activation site of STAT5 (Tyr 694 in STAT5A), and Src may contribute to EPO-induced signal transduction via STAT5. Tyrosine 22-30 signal transducer and activator of transcription 5A Homo sapiens 68-73 11641791-12 2001 Src can thus directly tyrosine-phosphorylate the activation site of STAT5 (Tyr 694 in STAT5A), and Src may contribute to EPO-induced signal transduction via STAT5. Tyrosine 22-30 signal transducer and activator of transcription 5A Homo sapiens 86-92 11641791-12 2001 Src can thus directly tyrosine-phosphorylate the activation site of STAT5 (Tyr 694 in STAT5A), and Src may contribute to EPO-induced signal transduction via STAT5. Tyrosine 22-30 signal transducer and activator of transcription 5A Homo sapiens 86-91 11641791-12 2001 Src can thus directly tyrosine-phosphorylate the activation site of STAT5 (Tyr 694 in STAT5A), and Src may contribute to EPO-induced signal transduction via STAT5. Tyrosine 75-78 signal transducer and activator of transcription 5A Homo sapiens 68-73 11641791-12 2001 Src can thus directly tyrosine-phosphorylate the activation site of STAT5 (Tyr 694 in STAT5A), and Src may contribute to EPO-induced signal transduction via STAT5. Tyrosine 75-78 signal transducer and activator of transcription 5A Homo sapiens 86-92 11641791-12 2001 Src can thus directly tyrosine-phosphorylate the activation site of STAT5 (Tyr 694 in STAT5A), and Src may contribute to EPO-induced signal transduction via STAT5. Tyrosine 75-78 signal transducer and activator of transcription 5A Homo sapiens 86-91 11418005-7 2001 Angiotensin II also induced STAT1 and STAT5 tyrosine phosphorylation and nuclear translocation of activated STATs in a receptor subtype-specific manner. Tyrosine 44-52 signal transducer and activator of transcription 5A Homo sapiens 38-43 11470130-3 2001 We report age-related changes in the JAK-STAT signalling pathway that results in decreased tyrosine phosphorylation of STAT5. Tyrosine 91-99 signal transducer and activator of transcription 5A Homo sapiens 119-124 11564774-6 2001 IL-10 did not change the expression of mRNA of both GM-CSFR alpha-chain and beta-chain, but inhibited tyrosine phosphorylation of STAT5 and extracellular signal-regulated kinases 1 and 2 in the monocytes. Tyrosine 102-110 signal transducer and activator of transcription 5A Homo sapiens 130-135 11495699-8 2001 RESULTS: STAT-5 tyrosine phosphorylation was similarly induced by PRL and IL-3, with an additive effect detected in the presence of both stimuli. Tyrosine 16-24 signal transducer and activator of transcription 5A Homo sapiens 9-15 11395848-5 2001 Specific tyrosines in the intracellular portion of the receptor are phosphorylated and serve as a docking site for intracellular proteins, including one of the signal transducers and activators of transcription (STAT5). Tyrosine 9-18 signal transducer and activator of transcription 5A Homo sapiens 212-217 11448122-2 2001 Leptin (50-200 nM) significantly increased tyrosine phosphorylation of STAT cytoplasmic transcription factors STAT3 and STAT5b in a dose-dependent manner and produced a gel-shift with STAT3- and STAT5-specific oligonucleotides. Tyrosine 43-51 signal transducer and activator of transcription 5A Homo sapiens 120-125 11330544-6 2001 RESULTS: In human T lymphocytes, 16H5 attenuated both the tyrosine phosphorylation of STAT5 by IL-2 and the IL-2-induced DNA-binding of this transcription factor. Tyrosine 58-66 signal transducer and activator of transcription 5A Homo sapiens 86-91 11097834-3 2000 By coprecipitation and far Western blotting analysis, we demonstrate that Crk (I/II) binds to tyrosine phosphorylated STAT5 in cells stimulated by cytokines such as thrombopoietin (TPO) and interleukin-2 (IL-2). Tyrosine 94-102 signal transducer and activator of transcription 5A Homo sapiens 118-123 11124255-2 2001 Epo bioresponses are relayed efficiently by minimal receptor forms that retain a single Tyr-343 site for STAT5 binding, while forms that lack all cytoplasmic Tyr(P) sites activate JAK2 and the transcription of c-Myc plus presumed additional target genes. Tyrosine 88-91 signal transducer and activator of transcription 5A Homo sapiens 105-110 11245630-2 2001 In response to either cytokine, STAT5 was rapidly tyrosine phosphorylated and acquired interferon gamma activation site (GAS) DNA binding activity. Tyrosine 50-58 signal transducer and activator of transcription 5A Homo sapiens 32-37 11245630-3 2001 Tyrosine-phosphorylated STAT5 was associated with both cytosolic and nuclear cell fractions. Tyrosine 0-8 signal transducer and activator of transcription 5A Homo sapiens 24-29 10847629-7 2000 Tyrosine phosphorylation of both Stat5a and Stat5b was induced by IL-6. Tyrosine 0-8 signal transducer and activator of transcription 5A Homo sapiens 33-39 10976919-8 2000 In these cells, addition of T3 with ovine (o)PRL decreased the amounts of total and tyrosine-phosphorylated Stat5 in the cytoplasm compared with oPRL-treated cells. Tyrosine 84-92 signal transducer and activator of transcription 5A Homo sapiens 108-113 11046040-6 2000 Disruption of constitutive Jak3/Stat5 activation by AG-490 was demonstrated by inhibition of 1) tyrosine phosphorylation of Jak3, Stat5a (Tyr(694)), and Stat5b (Tyr(699)); 2) serine phosphorylation of Stat5a (Ser(726)) as determined by a novel phosphospecific Ab; and 3) Stat5a/b DNA binding to the Stat5-responsive beta-casein promoter. Tyrosine 96-104 signal transducer and activator of transcription 5A Homo sapiens 32-37 11046040-6 2000 Disruption of constitutive Jak3/Stat5 activation by AG-490 was demonstrated by inhibition of 1) tyrosine phosphorylation of Jak3, Stat5a (Tyr(694)), and Stat5b (Tyr(699)); 2) serine phosphorylation of Stat5a (Ser(726)) as determined by a novel phosphospecific Ab; and 3) Stat5a/b DNA binding to the Stat5-responsive beta-casein promoter. Tyrosine 138-141 signal transducer and activator of transcription 5A Homo sapiens 32-37 11046040-6 2000 Disruption of constitutive Jak3/Stat5 activation by AG-490 was demonstrated by inhibition of 1) tyrosine phosphorylation of Jak3, Stat5a (Tyr(694)), and Stat5b (Tyr(699)); 2) serine phosphorylation of Stat5a (Ser(726)) as determined by a novel phosphospecific Ab; and 3) Stat5a/b DNA binding to the Stat5-responsive beta-casein promoter. Tyrosine 161-164 signal transducer and activator of transcription 5A Homo sapiens 32-37 10834938-6 2000 In HC11 cells, NF-kappaB p50/p65 activity was inversely correlated with prolactin-induced STAT5 tyrosine phosphorylation, expression of endogenous beta-casein gene, and of a transfected beta-casein gene promoter reporter construct. Tyrosine 96-104 signal transducer and activator of transcription 5A Homo sapiens 90-95 10834938-7 2000 This indicates a negative cross talk between NF-kappaB and the prolactin receptor/JAK2/STAT5 activation pathway, which occurs at the level of STAT5 tyrosine phosphorylation. Tyrosine 148-156 signal transducer and activator of transcription 5A Homo sapiens 87-92 10834938-7 2000 This indicates a negative cross talk between NF-kappaB and the prolactin receptor/JAK2/STAT5 activation pathway, which occurs at the level of STAT5 tyrosine phosphorylation. Tyrosine 148-156 signal transducer and activator of transcription 5A Homo sapiens 142-147 10834938-8 2000 Our results provide evidence for a role of NF-kappaB in normal mammary gland development, and indicate its function as a negative regulator of beta-casein gene expression during pregnancy by interfering with STAT5 tyrosine phosphorylation. Tyrosine 214-222 signal transducer and activator of transcription 5A Homo sapiens 208-213 10642538-7 2000 The activation of both Src and Stat5 is dependent on the same tyrosine residues Tyr(579) and Tyr(581) in PDGF beta-R; thus the observed inhibition by Src might result from competition for binding of Stat5 to the receptor. Tyrosine 62-70 signal transducer and activator of transcription 5A Homo sapiens 31-36 11876995-8 2000 Dimerization and tyrosine phosphorylation of EpoR and tyrosine phosphorylation of JAK2 occurred in 1 min and tyrosine phosphorylation of STAT5 in 5 minutes after Epo stimulation. Tyrosine 17-25 signal transducer and activator of transcription 5A Homo sapiens 137-142 10812249-7 2000 However, TEL/PDGFbetaR can phosphorylate STAT5 in transiently transfected COS cells, suggesting that TEL/PDGFbetaR may itself be the kinase involved in tyrosine phosphorylation of STAT proteins. Tyrosine 152-160 signal transducer and activator of transcription 5A Homo sapiens 41-46 10777558-4 2000 Piceatannol, previously reported as a Syk/ZAP70-specific kinase inhibitor, selectively inhibits the tyrosine phosphorylation of STAT3 and STAT5, but not of STAT1 and STAT2. Tyrosine 100-108 signal transducer and activator of transcription 5A Homo sapiens 138-143 10744710-5 2000 We have previously shown that in HC11 mammary epithelial cells Stat5a is phosphorylated on Tyr(694) in a prolactin-sensitive manner, whereas serine phosphorylation is constitutive (Wartmann, M., Cella, N., Hofer, P., Groner, B., Xiuwen, L., Hennighausen, L., and Hynes, N. E. (1996) J. Biol. Tyrosine 91-94 signal transducer and activator of transcription 5A Homo sapiens 63-69 10642538-7 2000 The activation of both Src and Stat5 is dependent on the same tyrosine residues Tyr(579) and Tyr(581) in PDGF beta-R; thus the observed inhibition by Src might result from competition for binding of Stat5 to the receptor. Tyrosine 80-83 signal transducer and activator of transcription 5A Homo sapiens 31-36 10642538-7 2000 The activation of both Src and Stat5 is dependent on the same tyrosine residues Tyr(579) and Tyr(581) in PDGF beta-R; thus the observed inhibition by Src might result from competition for binding of Stat5 to the receptor. Tyrosine 80-83 signal transducer and activator of transcription 5A Homo sapiens 199-204 10642538-7 2000 The activation of both Src and Stat5 is dependent on the same tyrosine residues Tyr(579) and Tyr(581) in PDGF beta-R; thus the observed inhibition by Src might result from competition for binding of Stat5 to the receptor. Tyrosine 93-96 signal transducer and activator of transcription 5A Homo sapiens 31-36 10959420-9 2000 They might account for the observed enhancement of prolactin induced tyrosine phosphorylation of STAT5 by glucocorticoids. Tyrosine 69-77 signal transducer and activator of transcription 5A Homo sapiens 97-102 10654938-0 2000 A small amphipathic alpha-helical region is required for transcriptional activities and proteasome-dependent turnover of the tyrosine-phosphorylated Stat5. Tyrosine 125-133 signal transducer and activator of transcription 5A Homo sapiens 149-154 10654938-5 2000 With Stat5 isoforms, we have observed that tyrosine-phosphorylated carboxyl-truncated forms of Stat5 proteins were considerably more stable than phosphorylated wild-type forms of the protein. Tyrosine 43-51 signal transducer and activator of transcription 5A Homo sapiens 5-10 10654938-5 2000 With Stat5 isoforms, we have observed that tyrosine-phosphorylated carboxyl-truncated forms of Stat5 proteins were considerably more stable than phosphorylated wild-type forms of the protein. Tyrosine 43-51 signal transducer and activator of transcription 5A Homo sapiens 95-100 10617656-0 2000 Cytosolic tyrosine dephosphorylation of STAT5. Tyrosine 10-18 signal transducer and activator of transcription 5A Homo sapiens 40-45 10959420-10 2000 For NF-kappa B and STAT5, one component of the antagonism is the inhibition of STAT5 tyrosine phosphorylation by activation of NF-kappa B. Tyrosine 85-93 signal transducer and activator of transcription 5A Homo sapiens 19-24 10959420-10 2000 For NF-kappa B and STAT5, one component of the antagonism is the inhibition of STAT5 tyrosine phosphorylation by activation of NF-kappa B. Tyrosine 85-93 signal transducer and activator of transcription 5A Homo sapiens 79-84 10428824-1 1999 In this study, DNA binding and tyrosine phosphorylation of STAT5A and STAT5B were compared with their subcellular localization determined using indirect immunofluorescence microscopy. Tyrosine 31-39 signal transducer and activator of transcription 5A Homo sapiens 59-65 10617656-4 2000 Tyrosine phosphorylation and dephosphorylation are critical in regulating STAT5 activity. Tyrosine 0-8 signal transducer and activator of transcription 5A Homo sapiens 74-79 10617656-6 2000 Using CTLL-20 as a model system, we provide evidence that tyrosine dephosphorylation of STAT5 subsequent to IL-2-induced phosphorylation occurs in the absence of STAT5 nuclear translocation and new protein synthesis. Tyrosine 58-66 signal transducer and activator of transcription 5A Homo sapiens 88-93 10617656-10 2000 Furthermore, tyrosine-phosphorylated STAT5 associates with the substrate-trapping mutant (Cys --> Ser) of SHP-2 but not SHP-1. Tyrosine 13-21 signal transducer and activator of transcription 5A Homo sapiens 37-42 10602027-5 2000 Both the A and B isoforms of STAT5 were found to associate with Tyr-510 of the IL-2Rbeta C-terminal region, depending on its phosphorylation. Tyrosine 64-67 signal transducer and activator of transcription 5A Homo sapiens 29-34 10729993-8 2000 We also cloned full-length cDNAs for STAT5s from three patients whose leukemic cells exhibited constitutive tyrosine phosphorylation of the STAT5 protein and expressed the derived STAT5 proteins in Ba/F3 cells. Tyrosine 108-116 signal transducer and activator of transcription 5A Homo sapiens 37-42 10729993-8 2000 We also cloned full-length cDNAs for STAT5s from three patients whose leukemic cells exhibited constitutive tyrosine phosphorylation of the STAT5 protein and expressed the derived STAT5 proteins in Ba/F3 cells. Tyrosine 108-116 signal transducer and activator of transcription 5A Homo sapiens 140-145 10729993-8 2000 We also cloned full-length cDNAs for STAT5s from three patients whose leukemic cells exhibited constitutive tyrosine phosphorylation of the STAT5 protein and expressed the derived STAT5 proteins in Ba/F3 cells. Tyrosine 108-116 signal transducer and activator of transcription 5A Homo sapiens 140-145 18726484-2 1999 It was found by using EMSA that during this period the signal transducer and activator of transcription 5 (STAT5) were tyrosine-phosphorylated and activated STAT5 may bind to v-interferon activated sequence (GAS). Tyrosine 119-127 signal transducer and activator of transcription 5A Homo sapiens 55-105 18726484-2 1999 It was found by using EMSA that during this period the signal transducer and activator of transcription 5 (STAT5) were tyrosine-phosphorylated and activated STAT5 may bind to v-interferon activated sequence (GAS). Tyrosine 119-127 signal transducer and activator of transcription 5A Homo sapiens 107-112 18726484-2 1999 It was found by using EMSA that during this period the signal transducer and activator of transcription 5 (STAT5) were tyrosine-phosphorylated and activated STAT5 may bind to v-interferon activated sequence (GAS). Tyrosine 119-127 signal transducer and activator of transcription 5A Homo sapiens 157-162 10512880-3 1999 Plating primary human endothelial cells from umbilical cord and the human endothelial cell line ECV304 on matrix proteins or on antibody to beta1- or alphav-integrin subunits induces transient tyrosine phosphorylation of JAK2 and STAT5A. Tyrosine 193-201 signal transducer and activator of transcription 5A Homo sapiens 230-236 10570284-4 1999 Although TSLP and IL-7 both induce tyrosine phosphorylation of the transcription factor Stat5, only IL-7-mediated signal transduction could be associated with activation of Janus family kinases (Jaks). Tyrosine 35-43 signal transducer and activator of transcription 5A Homo sapiens 88-93 10570284-5 1999 Because Stat5 phosphorylation following cytokine stimulation is generally mediated by Jaks, the lack of Jak activation after TSLP treatment suggested the possibility that tyrosine-phosphorylated Stat5 may be nonfunctional. Tyrosine 171-179 signal transducer and activator of transcription 5A Homo sapiens 195-200 10465259-8 1999 In addition, in human breast cancer cell T-47D, 20K-hGH was proved to stimulate Stat5 tyrosine phosphorylation to much lower degree than 22K-hGH via not hGHR but hPRLR. Tyrosine 86-94 signal transducer and activator of transcription 5A Homo sapiens 80-85 10373548-0 1999 SOCS-3 is tyrosine phosphorylated in response to interleukin-2 and suppresses STAT5 phosphorylation and lymphocyte proliferation. Tyrosine 10-18 signal transducer and activator of transcription 5A Homo sapiens 78-83 10419904-4 1999 In BCR-Abl transformed K562 cells, STAT5A and 5B are constitutively phosphorylated on tyrosine and are transcriptionally active. Tyrosine 86-94 signal transducer and activator of transcription 5A Homo sapiens 35-48 10358079-5 1999 In A431 cells, Stat1, Stat3, and Stat5, were constitutively complexed with ErbB1 and rapidly phosphorylated on tyrosine in response to EGF. Tyrosine 111-119 signal transducer and activator of transcription 5A Homo sapiens 33-38 10329850-8 1999 Adding back each tyrosine to Fall revealed that (1) Tyr577 was necessary and sufficient for Shc phosphorylation; (2) Tyr577, Tyr612, and Tyr695 were involved in activation of SHP-2, the Raf/ERK cascade, and c-fos transcription; and (3) all tyrosines, but particularly Tyr612, Tyr695, Tyr750, and Tyr806, facilitated STAT5 activation. Tyrosine 17-25 signal transducer and activator of transcription 5A Homo sapiens 316-321 10216087-5 1999 H2O2 induced a dose-dependent increase in tyrosine phosphorylation, including increased tyrosine phosphorylation of the GM-CSF receptor beta chain (betac), STAT5, and other signaling proteins. Tyrosine 42-50 signal transducer and activator of transcription 5A Homo sapiens 156-161 10084588-7 1999 Moreover, in GH-induced tyrosine phosphorylation of signal transducer and activator of transcription-5 (STAT5), GHR-277 exerted a dominant negative effect when GHR-277 and GHR-fl were cotransfected. Tyrosine 24-32 signal transducer and activator of transcription 5A Homo sapiens 52-102 10372132-4 1999 Mutation of each of the 8 tyrosine residues in the cytoplasmic domain of the human GMR beta to phenylalanine (GMR beta-F8) reduced tyrosine phosphorylation of GMR beta, SHP2 and SHC, but not JAK2 or STAT5. Tyrosine 26-34 signal transducer and activator of transcription 5A Homo sapiens 199-204 10068671-9 1999 IFN-alpha enhanced tyrosine phosphorylation of STAT1, STAT3, STAT4, STAT5a, and STAT5b. Tyrosine 19-27 signal transducer and activator of transcription 5A Homo sapiens 68-74 10085104-3 1999 Cellular depletion of Janus kinase-2 (JAK-2) using antisense oligodeoxynucleotides (ODNs) prevented GH-stimulated JAK-2 and signal transducer and activator of transcription (STAT)-5 tyrosine phosphorylation and severely attenuated the ability of GH to promote differentiation. Tyrosine 182-190 signal transducer and activator of transcription 5A Homo sapiens 124-181 10084588-7 1999 Moreover, in GH-induced tyrosine phosphorylation of signal transducer and activator of transcription-5 (STAT5), GHR-277 exerted a dominant negative effect when GHR-277 and GHR-fl were cotransfected. Tyrosine 24-32 signal transducer and activator of transcription 5A Homo sapiens 104-109 9973378-4 1999 IL-4 suppressed activation of DNA binding and tyrosine phosphorylation of the transcription factor Stat5 by IL-2, and suppressed the expression of the IL-2-inducible genes CD25, CIS, the PGE2 receptor, and cytokine responsive (CR) genes CR1 and CR8. Tyrosine 46-54 signal transducer and activator of transcription 5A Homo sapiens 99-104 10195568-7 1999 EPO-dependent tyrosine phosphorylation of Stat 5 also increased, but the EPO receptor (EPO-R) expression remained unchanged in the CD34+/CD45+ cells treated with SCF + HGF. Tyrosine 14-22 signal transducer and activator of transcription 5A Homo sapiens 42-48 9872990-2 1999 We report that Stat5 interacts constitutively with the IFN receptor-associated Tyk-2 kinase, and during IFNalpha stimulation its tyrosine-phosphorylated form acts as a docking site for the SH2 domain of CrkL. Tyrosine 129-137 signal transducer and activator of transcription 5A Homo sapiens 15-20 10037026-8 1999 STAT1, STAT3, and STAT5 underwent tyrosine phosphorylation when co-expressed with Jakl and therefore are substrates for the respective Jak kinases. Tyrosine 34-42 signal transducer and activator of transcription 5A Homo sapiens 18-23 10037026-9 1999 In contrast, Jak3 co-expression resulted in tyrosine phosphorylation of STAT3 and STAT5 but not STAT1. Tyrosine 44-52 signal transducer and activator of transcription 5A Homo sapiens 82-87 9973262-0 1999 Glucocorticoid receptor/signal transducer and activator of transcription 5 (STAT5) interactions enhance STAT5 activation by prolonging STAT5 DNA binding and tyrosine phosphorylation. Tyrosine 157-165 signal transducer and activator of transcription 5A Homo sapiens 24-74 9973262-0 1999 Glucocorticoid receptor/signal transducer and activator of transcription 5 (STAT5) interactions enhance STAT5 activation by prolonging STAT5 DNA binding and tyrosine phosphorylation. Tyrosine 157-165 signal transducer and activator of transcription 5A Homo sapiens 76-81 9973262-0 1999 Glucocorticoid receptor/signal transducer and activator of transcription 5 (STAT5) interactions enhance STAT5 activation by prolonging STAT5 DNA binding and tyrosine phosphorylation. Tyrosine 157-165 signal transducer and activator of transcription 5A Homo sapiens 104-109 9973262-0 1999 Glucocorticoid receptor/signal transducer and activator of transcription 5 (STAT5) interactions enhance STAT5 activation by prolonging STAT5 DNA binding and tyrosine phosphorylation. Tyrosine 157-165 signal transducer and activator of transcription 5A Homo sapiens 104-109 9973262-8 1999 This was correlated with increased STAT5 tyrosine phosphorylation, suggesting that GR enhances STAT5 DNA binding by modulating the rate of STAT5 dephosphorylation. Tyrosine 41-49 signal transducer and activator of transcription 5A Homo sapiens 35-40 9973262-8 1999 This was correlated with increased STAT5 tyrosine phosphorylation, suggesting that GR enhances STAT5 DNA binding by modulating the rate of STAT5 dephosphorylation. Tyrosine 41-49 signal transducer and activator of transcription 5A Homo sapiens 95-100 9973262-8 1999 This was correlated with increased STAT5 tyrosine phosphorylation, suggesting that GR enhances STAT5 DNA binding by modulating the rate of STAT5 dephosphorylation. Tyrosine 41-49 signal transducer and activator of transcription 5A Homo sapiens 95-100 9973262-9 1999 In contrast, cotransfection of the estrogen receptor resulted in an overall decrease in STAT5 tyrosine phosphorylation, without changing the kinetics of dephosphorylation. Tyrosine 94-102 signal transducer and activator of transcription 5A Homo sapiens 88-93 9880255-2 1999 STAT5 became immediately and transiently phosphorylated on tyrosine 694 in response to TCR stimulation. Tyrosine 59-67 signal transducer and activator of transcription 5A Homo sapiens 0-5 9813040-7 1998 Last, progesterone pretreatment enhances the phosphorylation of Stat5 on tyrosine 694 induced by epidermal growth factor. Tyrosine 73-81 signal transducer and activator of transcription 5A Homo sapiens 64-69 9845531-9 1998 Coincident with STAT5 tyrosine phosphorylation, thrombopoietin induces activation of STAT5 DNA-binding activity as demonstrated by electrophoretic mobility shift assays (EMSA). Tyrosine 22-30 signal transducer and activator of transcription 5A Homo sapiens 16-21 9845531-9 1998 Coincident with STAT5 tyrosine phosphorylation, thrombopoietin induces activation of STAT5 DNA-binding activity as demonstrated by electrophoretic mobility shift assays (EMSA). Tyrosine 22-30 signal transducer and activator of transcription 5A Homo sapiens 85-90 9885438-10 1998 These results show cytokine-specific and amplified tyrosine phosphorylation of proteins in AML cells and suggest that amplified response might, at least in part, result from the increased amount of signaling molecules such as Stat5. Tyrosine 51-59 signal transducer and activator of transcription 5A Homo sapiens 226-231 9862674-2 1998 Activation of Stat1 and Stat5 by G-CSF requires the membrane-proximal cytoplasmic domain of the receptor, including box1 and box2, while G-CSF-stimulated tyrosine phosphorylation of Stat3 also requires a region distal to box 2. Tyrosine 154-162 signal transducer and activator of transcription 5A Homo sapiens 24-29 9849880-0 1998 Constitutive association of JAK1 and STAT5 in pro-B cells is dissolved by interleukin-4-induced tyrosine phosphorylation of both proteins. Tyrosine 96-104 signal transducer and activator of transcription 5A Homo sapiens 37-42 9722506-8 1998 In addition, the inducible expression of dn-Ras abolished the IGF-I-enhanced tyrosine phosphorylation of STAT5. Tyrosine 77-85 signal transducer and activator of transcription 5A Homo sapiens 105-110 9669026-4 1998 Both STAT1 and STAT5 in this cell line are tyrosine-phosphorylated and translocated to nucleus following the binding of EPO to HEL cells. Tyrosine 43-51 signal transducer and activator of transcription 5A Homo sapiens 15-20 9722506-4 1998 The treatment of F-36P cells with a combination of EPO and IGF-I (EPO/IGF-I) was found to enhance EPO-induced tyrosine phosphorylation of STAT5, whereas IGF-I alone did not. Tyrosine 110-118 signal transducer and activator of transcription 5A Homo sapiens 138-143 9722506-9 1998 These results suggest that IGF-I may augment EPO-induced proliferation by enhancing tyrosine phosphorylation of STAT5 and raise the possibility that Ras may be involved in the augmentation of STAT5 tyrosyl phosphorylation. Tyrosine 84-92 signal transducer and activator of transcription 5A Homo sapiens 112-117 9633897-6 1998 We examined the phosphorylation of STAT 3 and STAT 5 at the tyrosine residues in AML samples in which MAP kinase activity had already been found. Tyrosine 60-68 signal transducer and activator of transcription 5A Homo sapiens 46-52 9633897-7 1998 40/50 primary AML cases (80%) exhibited constitutive tyrosine phosphorylation of STAT5. Tyrosine 53-61 signal transducer and activator of transcription 5A Homo sapiens 81-86 9633897-8 1998 Electrophoretic mobility shift assay showed DNA binding activity of STAT5 correlated with tyrosine phosphorylation of STAT5. Tyrosine 90-98 signal transducer and activator of transcription 5A Homo sapiens 68-73 9633897-8 1998 Electrophoretic mobility shift assay showed DNA binding activity of STAT5 correlated with tyrosine phosphorylation of STAT5. Tyrosine 90-98 signal transducer and activator of transcription 5A Homo sapiens 118-123 9722506-0 1998 Insulin-like growth factor-I augments erythropoietin-induced proliferation through enhanced tyrosine phosphorylation of STAT5. Tyrosine 92-100 signal transducer and activator of transcription 5A Homo sapiens 120-125 9657743-0 1998 Erythropoietin induces tyrosine phosphorylation of Jak2, STAT5A, and STAT5B in primary cultured human erythroid precursors. Tyrosine 23-31 signal transducer and activator of transcription 5A Homo sapiens 57-63 9657743-2 1998 We found that erythropoietin induces tyrosine phosphorylation of Jak2, STAT5A, and STAT5B. Tyrosine 37-45 signal transducer and activator of transcription 5A Homo sapiens 71-77 9657743-4 1998 Tyrosine phosphorylation of STAT5 is accompanied by the translocation of activated STAT5 to the nucleus as shown by electrophoretic mobility shift assay (EMSA) using 32Pi-labeled STAT5 binding site in the beta-casein promoter. Tyrosine 0-8 signal transducer and activator of transcription 5A Homo sapiens 28-33 9657743-4 1998 Tyrosine phosphorylation of STAT5 is accompanied by the translocation of activated STAT5 to the nucleus as shown by electrophoretic mobility shift assay (EMSA) using 32Pi-labeled STAT5 binding site in the beta-casein promoter. Tyrosine 0-8 signal transducer and activator of transcription 5A Homo sapiens 83-88 9657743-4 1998 Tyrosine phosphorylation of STAT5 is accompanied by the translocation of activated STAT5 to the nucleus as shown by electrophoretic mobility shift assay (EMSA) using 32Pi-labeled STAT5 binding site in the beta-casein promoter. Tyrosine 0-8 signal transducer and activator of transcription 5A Homo sapiens 83-88 9632771-6 1998 The mutant STAT5 was constitutively phosphorylated on tyrosine residues, localized in the nucleus, and was transcriptionally active. Tyrosine 54-62 signal transducer and activator of transcription 5A Homo sapiens 11-16 9637481-5 1998 Analysis of T cell lysates by immunoprecipitation with specific Abs and subsequent immunoblotting indicated marked reduction of tyrosine phosphorylation of Jak3 and STAT5 mediated by NO. Tyrosine 128-136 signal transducer and activator of transcription 5A Homo sapiens 165-170 9516478-7 1998 Furthermore, using different forms natural forms of the PRLR as well as receptor tyrosine to phenylalanine mutant forms, we determined that tyrosine phosphorylation of Stat5 is independent of PRLR phosphotyrosines. Tyrosine 140-148 signal transducer and activator of transcription 5A Homo sapiens 168-173 9544991-11 1998 We propose that SHP-2 relieves an inhibitory tyrosine phosphorylation event in Jak2 required for Jak2 activity, Stat5 phosphorylation, and transcriptional induction. Tyrosine 45-53 signal transducer and activator of transcription 5A Homo sapiens 112-117 9664155-4 1998 EPO activates the JAK2-STAT5 pathway, and two tyrosine residues (Y343, Y401) in the cytoplasmic domain of EPOR are important for STAT5 activation. Tyrosine 46-54 signal transducer and activator of transcription 5A Homo sapiens 129-134 9528750-6 1998 Lactogenic hormone treatment of HC11 mammary cells resulted in tyrosine phosphorylation of Stat5a and Stat5b, dimerization, and rapid nuclear translocation of both Stat5 proteins. Tyrosine 63-71 signal transducer and activator of transcription 5A Homo sapiens 91-97 9528750-6 1998 Lactogenic hormone treatment of HC11 mammary cells resulted in tyrosine phosphorylation of Stat5a and Stat5b, dimerization, and rapid nuclear translocation of both Stat5 proteins. Tyrosine 63-71 signal transducer and activator of transcription 5A Homo sapiens 91-96 9516478-0 1998 Prolactin receptor regulates Stat5 tyrosine phosphorylation and nuclear translocation by two separate pathways. Tyrosine 35-43 signal transducer and activator of transcription 5A Homo sapiens 29-34 9516478-7 1998 Furthermore, using different forms natural forms of the PRLR as well as receptor tyrosine to phenylalanine mutant forms, we determined that tyrosine phosphorylation of Stat5 is independent of PRLR phosphotyrosines. Tyrosine 81-89 signal transducer and activator of transcription 5A Homo sapiens 168-173 9516478-8 1998 We established, however, that the C-terminal tyrosine of the PRLR Nb2 form, Tyr382, plays an essential positive role in PRLR-dependent Stat5 nuclear translocation and subsequently DNA binding. Tyrosine 45-53 signal transducer and activator of transcription 5A Homo sapiens 135-140 9516478-9 1998 All together, our data propose a new model for activation of Stat5 through the PRLR, suggesting that Stat5 tyrosine phosphorylation and nuclear translocation are two separately regulated events. Tyrosine 107-115 signal transducer and activator of transcription 5A Homo sapiens 61-66 9516478-9 1998 All together, our data propose a new model for activation of Stat5 through the PRLR, suggesting that Stat5 tyrosine phosphorylation and nuclear translocation are two separately regulated events. Tyrosine 107-115 signal transducer and activator of transcription 5A Homo sapiens 101-106 9454765-3 1998 In this report, we show that STAT5, STAT1alpha, and STAT1beta, in addition to STAT4, are tyrosine phosphorylated in response to IL-12 in phytohemagglutinin (PHA)-activated human T cells. Tyrosine 89-97 signal transducer and activator of transcription 5A Homo sapiens 29-34 9454765-5 1998 The IL-12-induced tyrosine phosphorylation of STAT5 and STAT1, but not STAT4, is augmented in T cells activated into Th1 cells with PHA + interferon-gamma (IFN-gamma) compared with T cells activated with PHA alone. Tyrosine 18-26 signal transducer and activator of transcription 5A Homo sapiens 46-51 9613985-1 1998 IL-2R signal transduction involves tyrosine phosphorylation of several proteins including Jak3 and STAT5. Tyrosine 35-43 signal transducer and activator of transcription 5A Homo sapiens 99-104 9613985-6 1998 Furthermore, both CT compounds inhibited constitutive tyrosine phosphorylation of two proteins: Jak3 and STAT5 which are key downstream elements in the signal transduction pathway activated by IL-2 and the other cytokines. Tyrosine 54-62 signal transducer and activator of transcription 5A Homo sapiens 105-110 9484840-3 1998 Activation of the PDGF beta-receptor led to tyrosine phosphorylation of Stat1, Stat3 and Stat5, which was accompanied by specific DNA-binding activities. Tyrosine 44-52 signal transducer and activator of transcription 5A Homo sapiens 89-94 9484840-6 1998 Immobilized peptides containing phosphorylated Tyr579, Tyr581 or Tyr775 bound Stat5, suggesting direct binding of Stat5 to these tyrosine residues of the PDGF beta-receptor. Tyrosine 129-137 signal transducer and activator of transcription 5A Homo sapiens 114-119 9422769-6 1998 Furthermore, GM-CSF induced the tyrosine phosphorylation of STAT3 and STAT5 but not of STAT1, STAT2, STAT4, or STAT6. Tyrosine 32-40 signal transducer and activator of transcription 5A Homo sapiens 70-75 9422769-8 1998 STAT5 presented a different pattern of tyrosine phosphorylation. Tyrosine 39-47 signal transducer and activator of transcription 5A Homo sapiens 0-5 9422769-10 1998 The 94-kDa STAT5 was constitutively tyrosine phosphorylated and showed no change upon GM-CSF stimulation. Tyrosine 36-44 signal transducer and activator of transcription 5A Homo sapiens 11-16 9422769-11 1998 On the other hand, the 92-kDa STAT5 was tyrosine phosphorylated within 1 min of GM-CSF treatment and this was maintained for at least 30 min. Tyrosine 40-48 signal transducer and activator of transcription 5A Homo sapiens 30-35 9374508-5 1997 The STAT5-JAK2 proteins exhibit tyrosine kinase activity and are phosphorylated on tyrosine. Tyrosine 32-40 signal transducer and activator of transcription 5A Homo sapiens 4-9 9398404-5 1997 IL-2 and IL-7 were equivalent in their ability to induce tyrosine phosphorylation of STAT5A and STAT5B and to facilitate binding of these STATs to an immobilized GAS element. Tyrosine 57-65 signal transducer and activator of transcription 5A Homo sapiens 85-91 9343435-11 1997 Activation of Stat5 through phosphorylation of tyrosine 694 is an absolute prerequisite for transcription. Tyrosine 47-55 signal transducer and activator of transcription 5A Homo sapiens 14-19 9325013-8 1997 The relative level of detection of tyrosine phosphorylated JAK-2, STAT-5, SHC, and other substrates corresponded to the overall tyrosine phosphorylation signal. Tyrosine 35-43 signal transducer and activator of transcription 5A Homo sapiens 66-72 9310468-5 1997 However, the tyrosine phosphorylation of JAK2, the EPO receptor, and Stat5 was efficiently activated, and HCP was observed to associate constitutively with the EPO receptor in this differentiation-specific system. Tyrosine 13-21 signal transducer and activator of transcription 5A Homo sapiens 69-74 9325013-8 1997 The relative level of detection of tyrosine phosphorylated JAK-2, STAT-5, SHC, and other substrates corresponded to the overall tyrosine phosphorylation signal. Tyrosine 128-136 signal transducer and activator of transcription 5A Homo sapiens 66-72 9162019-3 1997 We report here that IL-2-induced DNA-binding activity and tyrosine phosphorylation of STAT5 are stabilized by a proteasome inhibitor MG132; however, no detectable ubiquitination of the STAT proteins is observed. Tyrosine 58-66 signal transducer and activator of transcription 5A Homo sapiens 86-91 9257712-0 1997 Multiple tyrosine residues in the intracellular domain of the common beta subunit of the interleukin 5 receptor are involved in activation of STAT5. Tyrosine 9-17 signal transducer and activator of transcription 5A Homo sapiens 142-147 9257712-3 1997 Our results indicate that: (a) tyrosine phosphorylation of the IL-5R beta chain is required for endogenous STAT5 activation, (b) multiple tyrosine residues are phosphorylated upon IL-5 stimulation, including Tyr577, Tyr612, Tyr695, and Tyr750, and (c) Tyr612, Tyr695, and Tyr750 are all capable of inducing activation of STAT5, demonstrating a high level of functional redundancy within the IL-5R beta chain. Tyrosine 31-39 signal transducer and activator of transcription 5A Homo sapiens 107-112 9182578-5 1997 We now report that IL2 stimulated marked phosphorylation of serine and tyrosine residues of both Stat5a and Stat5b in human T lymphocytes and in several IL2-responsive lymphocytic cell lines. Tyrosine 71-79 signal transducer and activator of transcription 5A Homo sapiens 97-103 9162019-4 1997 This sustained STAT5 activation can be blocked by protein kinase inhibitors, which is consistent with the ability of the proteasome inhibitor to stabilize IL-2-induced tyrosine phosphorylation of Jak1 and Jak3. Tyrosine 168-176 signal transducer and activator of transcription 5A Homo sapiens 15-20 9129017-8 1997 In HEK293 cells expressing STAT5 and the EPO receptor, EPO-dependent tyrosine phosphorylation of STAT5, as well as EPO-dependent CIS-promoter activation, was suppressed when CIS was coexpressed. Tyrosine 69-77 signal transducer and activator of transcription 5A Homo sapiens 27-32 9164861-3 1997 Furthermore it stimulated tyrosine phosphorylation of two isoforms of the transcriptional activator STAT5, STAT5a and STAT5b. Tyrosine 26-34 signal transducer and activator of transcription 5A Homo sapiens 100-105 9164861-3 1997 Furthermore it stimulated tyrosine phosphorylation of two isoforms of the transcriptional activator STAT5, STAT5a and STAT5b. Tyrosine 26-34 signal transducer and activator of transcription 5A Homo sapiens 107-113 9168989-2 1997 Recently both the tyrosine residues in the EPOR responsible for the activation of Stat5 and the role of Stat5 for EPO-dependent cell proliferation have been shown. Tyrosine 18-26 signal transducer and activator of transcription 5A Homo sapiens 82-87 9129017-8 1997 In HEK293 cells expressing STAT5 and the EPO receptor, EPO-dependent tyrosine phosphorylation of STAT5, as well as EPO-dependent CIS-promoter activation, was suppressed when CIS was coexpressed. Tyrosine 69-77 signal transducer and activator of transcription 5A Homo sapiens 97-102 9111009-1 1997 It has been previously demonstrated that growth hormone (GH)-stimulated tyrosine phosphorylation of Jak2 and Stat5a and Stat5b occurs in FDP-C1 cells expressing either the entire GH receptor or truncations of the cytoplasmic domain expressing only the membrane-proximal 80 amino acids. Tyrosine 72-80 signal transducer and activator of transcription 5A Homo sapiens 109-115 8977235-5 1996 Mutants lacking any tyrosine residues in the cytoplasmic domain maintain their ability to activate STAT5 and STAT1 but cannot activate STAT3, implying that STAT5 and STAT1 activation does not require receptor tyrosine phosphorylation. Tyrosine 20-28 signal transducer and activator of transcription 5A Homo sapiens 99-104 9108397-2 1997 To investigate the signaling processes induced by thrombopoietin, we have employed human platelets and recently demonstrated that thrombopoietin induces rapid tyrosine phosphorylation of Jak-2, Tyk2, Shc, Stat3, Stat5, p120(c-cbl) and other proteins in human platelets. Tyrosine 159-167 signal transducer and activator of transcription 5A Homo sapiens 212-217 9209327-7 1997 JAK1 and STAT5 were constitutively tyrosine-phosphorylated but the DNA binding activity of STAT5 was not induced. Tyrosine 35-43 signal transducer and activator of transcription 5A Homo sapiens 9-14 9079629-5 1997 Mutant chimeric receptors retaining either Tyr343 or Tyr401 could activate STAT5, judged by tyrosine-phosphorylation of STAT5 and induction of CIS, a target gene of STAT5. Tyrosine 92-100 signal transducer and activator of transcription 5A Homo sapiens 75-80 9079629-5 1997 Mutant chimeric receptors retaining either Tyr343 or Tyr401 could activate STAT5, judged by tyrosine-phosphorylation of STAT5 and induction of CIS, a target gene of STAT5. Tyrosine 92-100 signal transducer and activator of transcription 5A Homo sapiens 120-125 9079629-5 1997 Mutant chimeric receptors retaining either Tyr343 or Tyr401 could activate STAT5, judged by tyrosine-phosphorylation of STAT5 and induction of CIS, a target gene of STAT5. Tyrosine 92-100 signal transducer and activator of transcription 5A Homo sapiens 120-125 8940193-3 1996 STAT5 and, to a lesser extent, STATs 1 and 3 were constitutively activated by tyrosine phosphorylation and induction of DNA binding activity in both P210 and P190(BCR/ABL)-transformed cells, but P190 differed in that it also prominently activated STAT6. Tyrosine 78-86 signal transducer and activator of transcription 5A Homo sapiens 0-5 8943229-3 1996 Prolactin induces a signaling event which involves tyrosine phosphorylation of the mammary gland factor, Stat5, a member of the family of signal transducers and activators of transcription (Stat). Tyrosine 51-59 signal transducer and activator of transcription 5A Homo sapiens 83-110 8943229-6 1996 Lactogenic hormone treatment resulted in the appearance of a tyrosine-phosphorylated peptide in both Stat5 proteins. Tyrosine 61-69 signal transducer and activator of transcription 5A Homo sapiens 101-106 8943349-1 1996 Tyrosine phosphorylation and activation of the transcription factor Stat5 occur in response to stimuli like granulocyte-macrophage colony-stimulating factor, interleukin-3, or erythropoietin that stimulate both proliferation and differentiation of hematopoietic cells. Tyrosine 0-8 signal transducer and activator of transcription 5A Homo sapiens 68-73 8943349-7 1996 Tyrosine phosphorylation of Stat5 is not generally part of the IFN response. Tyrosine 0-8 signal transducer and activator of transcription 5A Homo sapiens 28-33 8704235-5 1996 Confocal microscopy revealed the nuclear localization of Stat5 and Western blot analyses showed tyrosine phosphorylation of Stat5 in nuclear extracts of acute leukemia cells. Tyrosine 96-104 signal transducer and activator of transcription 5A Homo sapiens 57-62 8808687-6 1996 We also showed a rapid and transient tyrosine phosphorylation of STAT5 in proliferating T47D cells which reached its peak after 30 min of Prl treatment. Tyrosine 37-45 signal transducer and activator of transcription 5A Homo sapiens 65-70 8756628-9 1996 Moreover, sequence homologies between human IL-9 receptor tyrosine 116 and tyrosines (of other receptors activating STAT3 and STAT5 were observed. Tyrosine 58-66 signal transducer and activator of transcription 5A Homo sapiens 126-131 8756628-9 1996 Moreover, sequence homologies between human IL-9 receptor tyrosine 116 and tyrosines (of other receptors activating STAT3 and STAT5 were observed. Tyrosine 75-84 signal transducer and activator of transcription 5A Homo sapiens 126-131 8710869-0 1996 Multiple tyrosine residues in the cytosolic domain of the erythropoietin receptor promote activation of STAT5. Tyrosine 9-17 signal transducer and activator of transcription 5A Homo sapiens 104-109 8710869-3 1996 By mutating the eight tyrosine residues in the cytosolic domain of the EPO-R, we show that either Y343 or Y401 is sufficient to mediate maximal activation of STAT5; tyrosine residues Y429 and Y431 can partially activate STAT5. Tyrosine 22-30 signal transducer and activator of transcription 5A Homo sapiens 158-163 8710869-3 1996 By mutating the eight tyrosine residues in the cytosolic domain of the EPO-R, we show that either Y343 or Y401 is sufficient to mediate maximal activation of STAT5; tyrosine residues Y429 and Y431 can partially activate STAT5. Tyrosine 165-173 signal transducer and activator of transcription 5A Homo sapiens 158-163 8874187-9 1996 STAT5 tyrosine phosphorylation was also observed in TF-1 cells. Tyrosine 6-14 signal transducer and activator of transcription 5A Homo sapiens 0-5 8695838-5 1996 Whereas 94-kD STAT5A was clearly tyrosine phosphorylated and bound to the enhancer element, the gamma response region (GRR), of the Fc gamma RI gene, substantially less tyrosine phosphorylated STAT5B bound to the immobilized GRR element. Tyrosine 33-41 signal transducer and activator of transcription 5A Homo sapiens 14-20 8702638-0 1996 An epidermal growth factor receptor/Jak2 tyrosine kinase domain chimera induces tyrosine phosphorylation of Stat5 and transduces a growth signal in hematopoietic cells. Tyrosine 41-49 signal transducer and activator of transcription 5A Homo sapiens 108-113 8704235-5 1996 Confocal microscopy revealed the nuclear localization of Stat5 and Western blot analyses showed tyrosine phosphorylation of Stat5 in nuclear extracts of acute leukemia cells. Tyrosine 96-104 signal transducer and activator of transcription 5A Homo sapiens 124-129 8647880-0 1996 Identification of tyrosine residues in the intracellular domain of the growth hormone receptor required for transcriptional signaling and Stat5 activation. Tyrosine 18-26 signal transducer and activator of transcription 5A Homo sapiens 138-143 8843416-2 1996 Using truncated and tyrosine mutants of the receptor, we show that different receptor domains are essential for the activation of Stat3 and Stat5. Tyrosine 20-28 signal transducer and activator of transcription 5A Homo sapiens 140-145 8843416-5 1996 In contrast, C-terminal tyrosine residues of GHR are absolutely required for Stat5 activation. Tyrosine 24-32 signal transducer and activator of transcription 5A Homo sapiens 77-82 8843416-7 1996 Using in vitro experiments with glutathione-S-transferase fusion proteins, we demonstrate that the SH2 domain of Stat5 binds to the carboxy-terminal tyrosine-phosphorylated residues of GHR. Tyrosine 149-157 signal transducer and activator of transcription 5A Homo sapiens 113-118 8647880-7 1996 This study demonstrates that specific tyrosines in the GH receptor are required for transcriptional signaling possibly by their role in the activation of transcription factor Stat5. Tyrosine 38-47 signal transducer and activator of transcription 5A Homo sapiens 175-180 8647880-6 1996 Activation of Stat5 by GH was, however, abolished in cells expressing the GH receptor lacking intracellular tyrosines. Tyrosine 108-117 signal transducer and activator of transcription 5A Homo sapiens 14-19 8631883-6 1996 This IL-2-induced Stat5 activation was dependent on the presence of either of two tyrosines (Tyr-392 or Tyr-510) in the IL-2 receptor beta chain, indicating that either of these two tyrosines can serve as a docking site. Tyrosine 82-91 signal transducer and activator of transcription 5A Homo sapiens 18-23 8631883-7 1996 Moreover, we demonstrated that human Stat5 activation is also dependent on Tyr-694 in Stat5A and Tyr-699 in Stat5B, indicating that these tyrosines are required for dimerization. Tyrosine 75-78 signal transducer and activator of transcription 5A Homo sapiens 37-42 8631883-7 1996 Moreover, we demonstrated that human Stat5 activation is also dependent on Tyr-694 in Stat5A and Tyr-699 in Stat5B, indicating that these tyrosines are required for dimerization. Tyrosine 75-78 signal transducer and activator of transcription 5A Homo sapiens 86-92 8631883-6 1996 This IL-2-induced Stat5 activation was dependent on the presence of either of two tyrosines (Tyr-392 or Tyr-510) in the IL-2 receptor beta chain, indicating that either of these two tyrosines can serve as a docking site. Tyrosine 93-96 signal transducer and activator of transcription 5A Homo sapiens 18-23 8631883-7 1996 Moreover, we demonstrated that human Stat5 activation is also dependent on Tyr-694 in Stat5A and Tyr-699 in Stat5B, indicating that these tyrosines are required for dimerization. Tyrosine 97-100 signal transducer and activator of transcription 5A Homo sapiens 37-42 8631883-6 1996 This IL-2-induced Stat5 activation was dependent on the presence of either of two tyrosines (Tyr-392 or Tyr-510) in the IL-2 receptor beta chain, indicating that either of these two tyrosines can serve as a docking site. Tyrosine 104-107 signal transducer and activator of transcription 5A Homo sapiens 18-23 8631883-7 1996 Moreover, we demonstrated that human Stat5 activation is also dependent on Tyr-694 in Stat5A and Tyr-699 in Stat5B, indicating that these tyrosines are required for dimerization. Tyrosine 138-147 signal transducer and activator of transcription 5A Homo sapiens 37-42 8631883-6 1996 This IL-2-induced Stat5 activation was dependent on the presence of either of two tyrosines (Tyr-392 or Tyr-510) in the IL-2 receptor beta chain, indicating that either of these two tyrosines can serve as a docking site. Tyrosine 182-191 signal transducer and activator of transcription 5A Homo sapiens 18-23 8657137-0 1996 Erythropoietin induces activation of Stat5 through association with specific tyrosines on the receptor that are not required for a mitogenic response. Tyrosine 77-86 signal transducer and activator of transcription 5A Homo sapiens 37-42 8732682-2 1996 We demonstrate here that GH activates the tyrosine phosphorylation of STAT5 in the human IM-9 lymphocyte cell line. Tyrosine 42-50 signal transducer and activator of transcription 5A Homo sapiens 70-75 8617237-4 1996 The two different phosphoforms of STAT5 have identical in vitro DNA binding specificity and reactivity with tyrosine phosphopeptides, but differ in their cellular localization. Tyrosine 108-116 signal transducer and activator of transcription 5A Homo sapiens 34-39 8657137-4 1996 The membrane-proximal domain contains a single tyrosine, Y-343, which when mutated eliminates the ability of the receptor to couple Epo binding to the activation of Stat5. Tyrosine 47-55 signal transducer and activator of transcription 5A Homo sapiens 165-170 8671609-3 1996 In the studies described here, we demonstrate that IL-5, IL-3 or GM-CSF stimulation induces the tyrosine phosphorylation of JAK2, and to a lesser extent JAK1, and of STAT5. Tyrosine 96-104 signal transducer and activator of transcription 5A Homo sapiens 166-171 8671609-6 1996 Intriguingly, electrophoretic mobility shift assay analysis revealed that STAT5 was activated in cells showing either JAK1 or JAK2 tyrosine phosphorylation. Tyrosine 131-139 signal transducer and activator of transcription 5A Homo sapiens 74-79 8671609-7 1996 These results indicate that activation of JAK1, JAK2 and STAT5 is critical to coupling IL-5-induced tyrosine phosphorylation and ultimately mitogenesis, and that Pro352 and Pro355 in the proline-rich sequence appear to play more essential roles in cell growth and in both JAK1/STAT5 and JAK2/STAT5 activation than Pro353 does. Tyrosine 100-108 signal transducer and activator of transcription 5A Homo sapiens 57-62 7543416-4 1995 The activation of both JAK2 and TYK2 was dose- and time-dependent and was associated with rapid tyrosine phosphorylation of a series of STAT proteins including STAT1, STAT3, and STAT5. Tyrosine 96-104 signal transducer and activator of transcription 5A Homo sapiens 178-183 8536642-2 1996 We have shown previously that in vivo GH administration rapidly modifies the tyrosine phosphorylation of multiple hepatic nuclear proteins, including the inducible transcription factors, Stat1, Stat3, and (in this report) Stat5, and have found that hormone treatment also rapidly alters gene transcription in the liver. Tyrosine 77-85 signal transducer and activator of transcription 5A Homo sapiens 222-227 7568001-4 1995 IL-2 and IL-15 rapidly induced the tyrosine phosphorylation of STAT3 and STAT5, and DNA-binding complexes containing STAT3 and STAT5 were rapidly activated by these cytokines in T cells. Tyrosine 35-43 signal transducer and activator of transcription 5A Homo sapiens 73-78 7544303-4 1995 STAT5 was a major component of this DNA-binding complex, and STAT5 was tyrosine phosphorylated in response to TPO. Tyrosine 71-79 signal transducer and activator of transcription 5A Homo sapiens 0-5 7544303-4 1995 STAT5 was a major component of this DNA-binding complex, and STAT5 was tyrosine phosphorylated in response to TPO. Tyrosine 71-79 signal transducer and activator of transcription 5A Homo sapiens 61-66 10388012-28 1996 At present, we know that the STAT whose activation is mediated by the EPO receptor is STAT5, and the target genes are CIS [6], which has an SH2 domain (a molecular structure that recognizes a phosphorylated tyrosine) and OSM [7], which is a pleiotropic cytokine. Tyrosine 207-215 signal transducer and activator of transcription 5A Homo sapiens 86-91 7479924-6 1995 The changes in the composition of IL-2Rs were accompanied by inhibition of IL-2-induced tyrosine phosphorylation of the Janus protein-tyrosine kinase 3 (Jak3) and signal transducers and activators of transcription called Stat3 and Stat5. Tyrosine 88-96 signal transducer and activator of transcription 5A Homo sapiens 231-236 8521813-0 1995 Tyrosine 343 in the erythropoietin receptor positively regulates erythropoietin-induced cell proliferation and Stat5 activation. Tyrosine 0-8 signal transducer and activator of transcription 5A Homo sapiens 111-116 8521813-2 1995 A comparison of Ep-induced proliferation with Ep-induced tyrosine phosphorylation patterns, using wild-type and Null EpR-expressing cells, revealed that Stat5 tyrosine phosphorylation and activation correlated directly with proliferation. Tyrosine 159-167 signal transducer and activator of transcription 5A Homo sapiens 153-158 7479881-4 1995 Activation of STAT5 is measured both by IL-2-induced tyrosine phosphorylation and by IL-2-induced DNA binding. Tyrosine 53-61 signal transducer and activator of transcription 5A Homo sapiens 14-19 7760829-7 1995 In contrast, IL-2, IL-3, and erythropoietin induce the tyrosine phosphorylation of Stat5 while IL-12 uniquely induces the tyrosine phosphorylation of Stat4. Tyrosine 55-63 signal transducer and activator of transcription 5A Homo sapiens 83-88 7608147-5 1995 The hematopoietic STAT5-L activated by IL-3, EPO, or PRL was identified as a 97-kDa tyrosine-phosphorylated protein. Tyrosine 84-92 signal transducer and activator of transcription 5A Homo sapiens 18-23 7796811-8 1995 The hematopoietic DNA binding activity related to STAT5 was identified as a p97 tyrosine-phosphorylated protein band which exhibited identical gel mobility to the mammary STAT5. Tyrosine 80-88 signal transducer and activator of transcription 5A Homo sapiens 171-176 7796811-8 1995 The hematopoietic DNA binding activity related to STAT5 was identified as a p97 tyrosine-phosphorylated protein band which exhibited identical gel mobility to the mammary STAT5. Tyrosine 80-88 signal transducer and activator of transcription 5A Homo sapiens 50-55 7744007-8 1995 The activation of DNA binding by prolactin, EPO and GH requires the phosphorylation of tyrosine residue 694 of MGF-Stat5. Tyrosine 87-95 signal transducer and activator of transcription 5A Homo sapiens 111-114 7744007-8 1995 The activation of DNA binding by prolactin, EPO and GH requires the phosphorylation of tyrosine residue 694 of MGF-Stat5. Tyrosine 87-95 signal transducer and activator of transcription 5A Homo sapiens 115-120 7925280-12 1994 Prolactin stimulation of transfected cells induces Stat5 phosphorylation on tyrosine. Tyrosine 76-84 signal transducer and activator of transcription 5A Homo sapiens 51-56 34665271-7 2022 The combinatorial treatments potentiated their suppressive effects on the tyrosine phosphorylation and stability of FLT3-ITD and its downstream signaling to the kinases ERK1/ERK2 and the inducible transcription factor STAT5. Tyrosine 74-82 signal transducer and activator of transcription 5A Homo sapiens 218-223 34188118-1 2021 In breast cancer, prolactin-induced activation of the transcription factor STAT5a results from the phosphorylation of STAT5a tyrosine residue 694. Tyrosine 125-133 signal transducer and activator of transcription 5A Homo sapiens 75-81 34188118-1 2021 In breast cancer, prolactin-induced activation of the transcription factor STAT5a results from the phosphorylation of STAT5a tyrosine residue 694. Tyrosine 125-133 signal transducer and activator of transcription 5A Homo sapiens 118-124