PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 15502848-0 2004 Novel roles of TLR3 tyrosine phosphorylation and PI3 kinase in double-stranded RNA signaling. Tyrosine 20-28 toll like receptor 3 Homo sapiens 15-19 15733829-6 2005 Specifically, we discuss the roles of receptor tyrosine phosphorylation, PI3 kinase and two-step activation of the transcription factors, IRF-3 and NF-kappaB, in mediating TLR3-signaling. Tyrosine 47-55 toll like receptor 3 Homo sapiens 172-176 15502848-4 2004 Here we report that dsRNA-activated phosphorylation of two specific tyrosine residues of TLR3 is essential for initiating two distinct signaling pathways. Tyrosine 68-76 toll like receptor 3 Homo sapiens 89-93 27226571-8 2016 The phosphorylation of TLR3 on tyrosine 759 was shown to increase in parallel to IFN-beta production in an iNOS- and Src-dependent manner, and Src was found to directly interact with TLR3 in the endosomal compartment of poly(I:C)-treated cells. Tyrosine 31-39 toll like receptor 3 Homo sapiens 23-27 14729660-7 2004 Treatment with in vitro transcribed mRNA activated nuclear factor-kappaB via TLR3 through a process that was dose-dependent and involved tyrosine phosphorylation. Tyrosine 137-145 toll like receptor 3 Homo sapiens 77-81 12509442-0 2003 Double-stranded RNA signaling by Toll-like receptor 3 requires specific tyrosine residues in its cytoplasmic domain. Tyrosine 72-80 toll like receptor 3 Homo sapiens 33-53 12509442-4 2003 Transfection assays, using a reporter gene driven by the 561 promoter, identified specific tyrosine residues to be essential for TLR3 signaling. Tyrosine 91-99 toll like receptor 3 Homo sapiens 129-133 12509442-5 2003 This conclusion was further validated in permanent cell lines expressing tyrosine-mutant TLR3 proteins; in some of these cell lines dsRNA failed to induce the 561 mRNA. Tyrosine 73-81 toll like receptor 3 Homo sapiens 89-93 12509442-6 2003 Our results provide the first demonstration of the importance of TLR3 cytoplasmic tyrosine residues in dsRNA signaling. Tyrosine 82-90 toll like receptor 3 Homo sapiens 65-69 33154351-5 2020 Subsequently, our data demonstrate that phosphorylation of TLR3 tyrosine residue and its interaction with PI3K is enhanced, and protein levels of phospho-PI3K and phospho-Akt are both increased after poly(I:C) pretreatment, while knock out of TLR3 suppresses the cardioprotection of poly(I:C) preconditioning through a decreased activation of PI3K/Akt signaling. Tyrosine 64-72 toll like receptor 3 Homo sapiens 59-63 29383749-7 2018 Our results reveal that phosphorylation of the Tyr 759 residue in the TIR domain of TLR3 introduces rigidity to its BB loop. Tyrosine 47-50 toll like receptor 3 Homo sapiens 84-88 17178723-0 2007 Two tyrosine residues of Toll-like receptor 3 trigger different steps of NF-kappa B activation. Tyrosine 4-12 toll like receptor 3 Homo sapiens 25-45 25022196-4 2014 Nevertheless, it is clear that many TLRs require, for signaling, ligand-dependent phosphorylation of specific Tyr residues in their cytoplasmic domains; the list includes TLR2, TLR3, TLR4, TLR5, TLR8 and TLR9. Tyrosine 110-113 toll like receptor 3 Homo sapiens 177-181 24526685-0 2014 The inducible nitric-oxide synthase (iNOS)/Src axis mediates Toll-like receptor 3 tyrosine 759 phosphorylation and enhances its signal transduction, leading to interferon-beta synthesis in macrophages. Tyrosine 82-90 toll like receptor 3 Homo sapiens 61-81 24526685-1 2014 Double-stranded RNA (dsRNA) induces phosphorylation of Toll-like receptor 3 (TLR3) at tyrosine 759 and subsequently triggers signaling pathways to promote interferon-beta (IFN-beta) production. Tyrosine 86-94 toll like receptor 3 Homo sapiens 55-75 24526685-1 2014 Double-stranded RNA (dsRNA) induces phosphorylation of Toll-like receptor 3 (TLR3) at tyrosine 759 and subsequently triggers signaling pathways to promote interferon-beta (IFN-beta) production. Tyrosine 86-94 toll like receptor 3 Homo sapiens 77-81 24526685-2 2014 In this study, we found that dsRNA stimulation induces biphasic TLR3 Tyr-759 phosphorylation in macrophages. Tyrosine 69-72 toll like receptor 3 Homo sapiens 64-68 24526685-4 2014 Interestingly, Src phosphorylated TLR3 Tyr-759 in vitro and in vivo. Tyrosine 39-42 toll like receptor 3 Homo sapiens 34-38 24526685-5 2014 However, knockdown of Src abolished the late phase of TLR3 Tyr-759 phosphorylation and decreased the nuclear accumulation of interferon regulatory factors 3 and 7 (IRF3 and -7) and IFN-beta production. Tyrosine 59-62 toll like receptor 3 Homo sapiens 54-58 24526685-7 2014 Notably, via down-regulation of Src, dsRNA-elicited TLR3 Tyr-759 phosphorylation, the nuclear accumulation of IRF3/IRF7, and IFN-beta generation were inhibited in inducible nitric-oxide synthase (iNOS)-null macrophages. Tyrosine 57-60 toll like receptor 3 Homo sapiens 52-56 22479513-4 2012 In this study we demonstrate that TRIM21 is tyrosine phosphorylated following TLR3 and TLR4 stimulation, suggesting that its activity is potentially regulated by tyrosine phosphorylation. Tyrosine 44-52 toll like receptor 3 Homo sapiens 78-82 22479513-4 2012 In this study we demonstrate that TRIM21 is tyrosine phosphorylated following TLR3 and TLR4 stimulation, suggesting that its activity is potentially regulated by tyrosine phosphorylation. Tyrosine 162-170 toll like receptor 3 Homo sapiens 78-82 22479513-8 2012 Our data demonstrates a novel role for tyrosine phosphorylation in regulating the activity of TRIM21 downstream of TLR3 and TLR4. Tyrosine 39-47 toll like receptor 3 Homo sapiens 115-119 22810896-4 2012 Unlike other TLRs, TLR3 requires phosphorylation of two specific tyrosine residues in its cytoplasmic domain to recruit the adaptor protein TRIF (Toll-interleukin-1 receptor domain-containing adaptor protein inducing interferon-beta) and initiate the antiviral response. Tyrosine 65-73 toll like receptor 3 Homo sapiens 19-23 22810896-5 2012 We showed that two protein tyrosine kinases, the epidermal growth factor receptor (EGFR) ErbB1 and Src, bound sequentially to dsRNA-activated TLR3 and phosphorylated the two tyrosine residues. Tyrosine 27-35 toll like receptor 3 Homo sapiens 142-146 17178723-2 2007 We demonstrated that dsRNA-induced phosphorylation of two specific tyrosine residues, 759 and 858, of TLR3 was necessary and sufficient for complete activation of the NF-kappaB pathway. Tyrosine 67-75 toll like receptor 3 Homo sapiens 102-106 17178723-3 2007 When Tyr-759 of TLR3 was mutated, gene induction was inhibited, although NF-kappaB was partially activated. Tyrosine 5-8 toll like receptor 3 Homo sapiens 16-20