PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 33217668-4 2021 We found that Euc effectively suppressed STAT3 activation at tyrosine 705, inhibited its translocation to nucleus, and decreased its DNA binding capacity. Tyrosine 61-69 signal transducer and activator of transcription 3 Homo sapiens 41-46 32868906-5 2021 In the two breast cancer cell lines, DCV selectively inhibited the phosphorylation of STAT3 Tyr-705, but did not affect the upstream components JAK1 and JAK2, as well as dephosphorylation of STAT3. Tyrosine 92-95 signal transducer and activator of transcription 3 Homo sapiens 86-91 33909625-6 2021 In this study, LLL12B inhibited STAT3 phosphorylation (tyrosine 705) and the expression of its downstream targets, which are associated with cancer cell proliferation and survival. Tyrosine 55-63 signal transducer and activator of transcription 3 Homo sapiens 32-37 33382511-4 2021 STAT3 is rapidly and transiently activated by tyrosine phosphorylation by a range of signalling pathways, including cytokines from the IL-6 family and growth factors, such as EGF and PDGF. Tyrosine 46-54 signal transducer and activator of transcription 3 Homo sapiens 0-5 33901593-9 2021 Our results demonstrate the proviral role of STAT3 for DV-2 propagation which is correlated to activation by tyrosine phosphorylation. Tyrosine 109-117 signal transducer and activator of transcription 3 Homo sapiens 45-50 33689932-6 2021 Further biochemical study using Western blot assay indicated that B19 blocked the phosphorylation of STAT3 at Tyr 705 and Ser 727 and thus suppressed STAT3-mediated gene expression of c-MYC and MCL-1. Tyrosine 110-113 signal transducer and activator of transcription 3 Homo sapiens 101-106 33053804-3 2020 STAT3 undergoes activation through phosphorylation on tyrosine 705, which facilitates its dimerization. Tyrosine 54-62 signal transducer and activator of transcription 3 Homo sapiens 0-5 33262462-5 2021 In summary, STAT3 phosphorylation at tyrosine 705 and serine 727 differentially regulates the EMT-MET switch within the distinct molecular subtypes of CSCs to complete the metastatic process. Tyrosine 37-45 signal transducer and activator of transcription 3 Homo sapiens 12-17 33066004-9 2020 Results showed that ILL decreased phosphorylation of serine 727 and tyrosine 705 of STAT3 and expression of survivin, a STAT3-regulated gene. Tyrosine 68-76 signal transducer and activator of transcription 3 Homo sapiens 84-89 32201262-2 2020 Elevated tyrosine phosphorylation (pY) of the latent transcription factor STAT3 is a feature of gastric cancer, including H. pylori-infected tissues, and is aligned to nuclear transcriptional activity. Tyrosine 9-17 signal transducer and activator of transcription 3 Homo sapiens 74-79 33042115-7 2020 Moreover, we showed that tyrosine residues 297 and 315 of TLT2 cytoplasmic domain were involved in STAT3 activation. Tyrosine 25-33 signal transducer and activator of transcription 3 Homo sapiens 99-104 33123602-7 2020 Focusing on the mechanism responsible for reduced phosphorylation levels of ERK and STAT3, we reveal that the activation of protein tyrosine phosphatase SHP-1 (Src homology region 2 domain-containing phosphatase-1) following hypoxia might play an essential role in this process. Tyrosine 132-140 signal transducer and activator of transcription 3 Homo sapiens 84-89 32598086-9 2020 A77 1726 inhibited JAK1, JAK2, and STAT3 tyrosine phosphorylation. Tyrosine 41-49 signal transducer and activator of transcription 3 Homo sapiens 35-40 32298693-5 2020 Mechanistic study revealed that pyrvinium dose-dependently suppressed STAT3 phosphorylation at tyrosine 705 and serine 727. Tyrosine 95-103 signal transducer and activator of transcription 3 Homo sapiens 70-75 31900385-3 2020 We showed that DDIAS promotes tyrosine phosphorylation of signal transducer and activator of transcription 3 (STAT3), which is constitutively activated in malignant cancers. Tyrosine 30-38 signal transducer and activator of transcription 3 Homo sapiens 58-108 32032581-6 2020 We show that ITE reduces the expression of JAG1 the amount of Notch 1 intracellular domain (NICD1) and the phosphorylation of STAT3 (at tyrosine 705) in TNBC MDA-MB-231 cells. Tyrosine 136-144 signal transducer and activator of transcription 3 Homo sapiens 126-131 31982491-4 2020 Western blot analysis and electrophoretic mobility shift assays (EMSA) showed that LYW-6 inhibited the phosphorylation of STAT3 tyrosine 705 (Tyr-705) and had slight effects on STAT1 and STAT5 phosphorylation. Tyrosine 128-136 signal transducer and activator of transcription 3 Homo sapiens 122-127 32207811-5 2020 The mutant alleles encode GP130 receptors bearing the transmembrane domain but lacking both the recycling motif and all four STAT3-recruiting tyrosine residues. Tyrosine 142-150 signal transducer and activator of transcription 3 Homo sapiens 125-130 32439998-5 2020 As such, the signalling properties of haematological transmembrane receptors rely on the membrane-proximal phosphotyrosine based sequence motifs (TBSMs) such as ITAM (immunoreceptor tyrosine-based activation motif), ITIM (immunoreceptor tyrosine-based inhibition motif) and signal transducer and activator of transcription 3 (STAT3)-recruiting YxxQ motifs. Tyrosine 114-122 signal transducer and activator of transcription 3 Homo sapiens 274-324 32439998-5 2020 As such, the signalling properties of haematological transmembrane receptors rely on the membrane-proximal phosphotyrosine based sequence motifs (TBSMs) such as ITAM (immunoreceptor tyrosine-based activation motif), ITIM (immunoreceptor tyrosine-based inhibition motif) and signal transducer and activator of transcription 3 (STAT3)-recruiting YxxQ motifs. Tyrosine 114-122 signal transducer and activator of transcription 3 Homo sapiens 326-331 32231398-2 2020 STAT3 has been found to be persistently activated in many types of cancers, primarily through its tyrosine phosphorylation (Y705). Tyrosine 98-106 signal transducer and activator of transcription 3 Homo sapiens 0-5 32030510-7 2020 RESULTS: We found that CRISPR-Cas9-mediated FOXO3 disruption in U87MG cells caused decreased OCT4 and SOX2 gene expression, STAT3 phosphorylation on tyrosine 705 and clonogenicity. Tyrosine 149-157 signal transducer and activator of transcription 3 Homo sapiens 124-129 31963765-2 2020 STAT3 and STAT5 are common targets for different tyrosine kinase oncogenes (TKOs). Tyrosine 49-57 signal transducer and activator of transcription 3 Homo sapiens 0-5 31900385-3 2020 We showed that DDIAS promotes tyrosine phosphorylation of signal transducer and activator of transcription 3 (STAT3), which is constitutively activated in malignant cancers. Tyrosine 30-38 signal transducer and activator of transcription 3 Homo sapiens 110-115 31900385-4 2020 Interestingly, siRNA protein tyrosine phosphatase (PTP) library screening revealed protein tyrosine phosphatase receptor mu (PTPRM) as a novel STAT3 PTP. Tyrosine 29-37 signal transducer and activator of transcription 3 Homo sapiens 143-148 31900385-4 2020 Interestingly, siRNA protein tyrosine phosphatase (PTP) library screening revealed protein tyrosine phosphatase receptor mu (PTPRM) as a novel STAT3 PTP. Tyrosine 91-99 signal transducer and activator of transcription 3 Homo sapiens 143-148 31499589-5 2020 KEY RESULTS: LL1 is highly selective among STATs family members and specifically inhibits phosphorylation of STAT3 Tyr-705 site, blocking the whole transmission process of STAT3 signaling. Tyrosine 115-118 signal transducer and activator of transcription 3 Homo sapiens 43-48 31499589-5 2020 KEY RESULTS: LL1 is highly selective among STATs family members and specifically inhibits phosphorylation of STAT3 Tyr-705 site, blocking the whole transmission process of STAT3 signaling. Tyrosine 115-118 signal transducer and activator of transcription 3 Homo sapiens 109-114 31499589-5 2020 KEY RESULTS: LL1 is highly selective among STATs family members and specifically inhibits phosphorylation of STAT3 Tyr-705 site, blocking the whole transmission process of STAT3 signaling. Tyrosine 115-118 signal transducer and activator of transcription 3 Homo sapiens 172-177 31956373-11 2020 In addition, deactivated mutagenesis of STAT3 the tyrosine 705 site had no effect on the aggregation of STAT3 into nucleus induced by nicotine. Tyrosine 50-58 signal transducer and activator of transcription 3 Homo sapiens 40-45 31678681-6 2020 We hypothesized that TCDD-mediated increases in SHP-1 could result in decreased STAT3 tyrosine phosphorylation. Tyrosine 86-94 signal transducer and activator of transcription 3 Homo sapiens 80-85 31878046-6 2019 Pervanadate (tyrosine phosphatase blocker) exposure altered the DDZ-induced inhibition of STAT3 activation, thus affecting the action of this phytoestrogen on apoptosis. Tyrosine 13-21 signal transducer and activator of transcription 3 Homo sapiens 90-95 31752959-6 2019 Promising clinical applications of an inhibitor of STAT3 tyrosine phosphorylation, C188-9, and specific blockade with CXCR4 antibody were explored. Tyrosine 57-65 signal transducer and activator of transcription 3 Homo sapiens 51-56 31861720-7 2019 Signal transducer and activator of transcription-3 (STAT3) and STAT5 are aberrantly activated through tyrosine phosphorylation in a wide variety of cancer types, including EOC. Tyrosine 102-110 signal transducer and activator of transcription 3 Homo sapiens 0-50 31861720-7 2019 Signal transducer and activator of transcription-3 (STAT3) and STAT5 are aberrantly activated through tyrosine phosphorylation in a wide variety of cancer types, including EOC. Tyrosine 102-110 signal transducer and activator of transcription 3 Homo sapiens 52-57 31557897-7 2019 On the other hand, several tumor suppressor pathways act on or are activated by STAT3/5 signaling, including tyrosine phosphatases, the sumo ligase Protein Inhibitor of Activated STAT3 (PIAS3), the E3 ubiquitin ligase TATA Element Modulatory Factor/Androgen Receptor-Coactivator of 160 kDa (TMF/ARA160), the miRNAs miR-124 and miR-1181, the Protein of alternative reading frame 19 (p19ARF)/p53 pathway and the Suppressor of Cytokine Signaling 1 and 3 (SOCS1/3) proteins. Tyrosine 109-117 signal transducer and activator of transcription 3 Homo sapiens 80-87 31481496-1 2019 Deregulated activation of the latent oncogenic transcription factor signal transducer and activator of transcription (STAT)3 in many human epithelial malignancies, including gastric cancer (GC), has invariably been associated with its canonical tyrosine phosphorylation and enhanced transcriptional activity. Tyrosine 245-253 signal transducer and activator of transcription 3 Homo sapiens 68-124 31537377-6 2019 Although mutation of Ser 782 to Ala or Cys in gp130 upregulates global epirubicin-induced autophagy, reduced degradation of gp130 accompanied with enhanced Stat3 phosphorylation at tyrosine 705 is observed. Tyrosine 181-189 signal transducer and activator of transcription 3 Homo sapiens 156-161 31684051-3 2019 ODZ10117 targeted the SH2 domain of STAT3 regardless of other STAT family proteins and upstream regulators of STAT3, leading to inhibition of the tyrosine phosphorylation, dimerization, nuclear translocation, and transcriptional activity of STAT3. Tyrosine 146-154 signal transducer and activator of transcription 3 Homo sapiens 36-41 31684051-3 2019 ODZ10117 targeted the SH2 domain of STAT3 regardless of other STAT family proteins and upstream regulators of STAT3, leading to inhibition of the tyrosine phosphorylation, dimerization, nuclear translocation, and transcriptional activity of STAT3. Tyrosine 146-154 signal transducer and activator of transcription 3 Homo sapiens 36-40 31427441-4 2019 Phosphorylated STAT3 (p-STAT3, Tyr 705) could bind to the promotor of ERR-alpha, and activate its transcription, which was suggested by luciferase assay and chromatin immunoprecipitation assay. Tyrosine 31-34 signal transducer and activator of transcription 3 Homo sapiens 15-20 31427441-4 2019 Phosphorylated STAT3 (p-STAT3, Tyr 705) could bind to the promotor of ERR-alpha, and activate its transcription, which was suggested by luciferase assay and chromatin immunoprecipitation assay. Tyrosine 31-34 signal transducer and activator of transcription 3 Homo sapiens 24-29 31427441-8 2019 Our finding demonstrated that ERR-alpha was a direct regulatory gene target of p-STAT3, which was enriched for processes involving invasion and metastasis in TNBC and provided insight into TNBC pathogenesis, as well as a potential therapeutic option against TNBC metastasis.Implications: Our research first showed that p-STAT3 (Tyr 705) could bind to the promotor region of ERR-alpha and promote EMT in TNBC by ZEB1 pathways, thus providing a potential clinical target for TNBC. Tyrosine 328-331 signal transducer and activator of transcription 3 Homo sapiens 81-86 31427441-8 2019 Our finding demonstrated that ERR-alpha was a direct regulatory gene target of p-STAT3, which was enriched for processes involving invasion and metastasis in TNBC and provided insight into TNBC pathogenesis, as well as a potential therapeutic option against TNBC metastasis.Implications: Our research first showed that p-STAT3 (Tyr 705) could bind to the promotor region of ERR-alpha and promote EMT in TNBC by ZEB1 pathways, thus providing a potential clinical target for TNBC. Tyrosine 328-331 signal transducer and activator of transcription 3 Homo sapiens 321-326 31619200-0 2019 STAT3 induces G9a to exacerbate HER3 expression for the survival of epidermal growth factor receptor-tyrosine kinase inhibitors in lung cancers. Tyrosine 101-109 signal transducer and activator of transcription 3 Homo sapiens 0-5 31557897-7 2019 On the other hand, several tumor suppressor pathways act on or are activated by STAT3/5 signaling, including tyrosine phosphatases, the sumo ligase Protein Inhibitor of Activated STAT3 (PIAS3), the E3 ubiquitin ligase TATA Element Modulatory Factor/Androgen Receptor-Coactivator of 160 kDa (TMF/ARA160), the miRNAs miR-124 and miR-1181, the Protein of alternative reading frame 19 (p19ARF)/p53 pathway and the Suppressor of Cytokine Signaling 1 and 3 (SOCS1/3) proteins. Tyrosine 109-117 signal transducer and activator of transcription 3 Homo sapiens 80-85 31346092-6 2019 This mutation creates a new exon in the STAT3 complementary DNA, which, when overexpressed, generates a mutant STAT3 protein (D427ins17) that is loss-of-function and dominant-negative in terms of tyrosine phosphorylation, DNA binding, and transcriptional activity. Tyrosine 196-204 signal transducer and activator of transcription 3 Homo sapiens 40-45 31170499-1 2019 The signal transducer and activator of transcription 3 (STAT3) protein is activated by phosphorylation of a specific tyrosine residue (Tyr705) in response to various extracellular signals. Tyrosine 117-125 signal transducer and activator of transcription 3 Homo sapiens 4-54 31170499-1 2019 The signal transducer and activator of transcription 3 (STAT3) protein is activated by phosphorylation of a specific tyrosine residue (Tyr705) in response to various extracellular signals. Tyrosine 117-125 signal transducer and activator of transcription 3 Homo sapiens 56-61 30910759-2 2019 This study investigates RAD23B and STAT3 gene perturbations in a large cohort of primary Sezary cells and the effect of FK228 treatment on tyrosine phosphorylation of STAT3 (pYSTAT3) and RAD23B expression. Tyrosine 139-147 signal transducer and activator of transcription 3 Homo sapiens 167-172 31462771-2 2019 Cytokines and growth factors activate STAT3 through kinase-mediated tyrosine phosphorylation and dimerization3,4. Tyrosine 68-76 signal transducer and activator of transcription 3 Homo sapiens 38-43 31491838-0 2019 Inhibition of Tyrosine-Phosphorylated STAT3 in Human Breast and Lung Cancer Cells by Manuka Honey is Mediated by Selective Antagonism of the IL-6 Receptor. Tyrosine 14-22 signal transducer and activator of transcription 3 Homo sapiens 38-43 31491838-1 2019 Aberrantly high levels of tyrosine-phosphorylated signal transducer and activator of transcription 3 (p-STAT3) are found constitutively in ~50% of human lung and breast cancers, acting as an oncogenic transcription factor. Tyrosine 26-34 signal transducer and activator of transcription 3 Homo sapiens 104-109 31409371-10 2019 In addition, STAT3 is tyrosine-phosphorylated in a biphasic manner, and the late phase requires ERalpha-36-mediated p300-dependent acetylation. Tyrosine 22-30 signal transducer and activator of transcription 3 Homo sapiens 13-18 31346092-6 2019 This mutation creates a new exon in the STAT3 complementary DNA, which, when overexpressed, generates a mutant STAT3 protein (D427ins17) that is loss-of-function and dominant-negative in terms of tyrosine phosphorylation, DNA binding, and transcriptional activity. Tyrosine 196-204 signal transducer and activator of transcription 3 Homo sapiens 111-116 31323961-5 2019 Mechanistic analyses further revealed that 5-AcTMF lowered the levels of Tyrosine 705-phosphorylated STAT3 (p-STAT3), a canonical marker of STAT3 activation, but also dampened p-STAT3 upregulation elicited by Interleukin-6. Tyrosine 73-81 signal transducer and activator of transcription 3 Homo sapiens 101-106 30981183-5 2019 CAG predominantly caused negative regulation of STAT3 phosphorylation at tyrosine 705 through the abrogation of Src and Janus-activated kinases (JAK1/2) activation. Tyrosine 73-81 signal transducer and activator of transcription 3 Homo sapiens 48-53 31277507-4 2019 This was accompanied by the tyrosine phosphorylation of STAT3, whereas treatment with pharmacological inhibitors of STAT3 suppressed the phosphorylation of STAT3 and the expression of intestinal maturation markers. Tyrosine 28-36 signal transducer and activator of transcription 3 Homo sapiens 56-61 31321245-0 2019 STAT3 Genotypic Variant rs744166 and Increased Tyrosine Phosphorylation of STAT3 in IL-23 Responsive Innate Lymphoid Cells during Pathogenesis of Crohn"s Disease. Tyrosine 47-55 signal transducer and activator of transcription 3 Homo sapiens 75-80 31321245-9 2019 Moreover, carriage of STAT3 "A" risk allele exhibited a higher basal level of STAT3 tyrosine phosphorylation, and an increased IL-23 triggered the pSTAT3 level. Tyrosine 84-92 signal transducer and activator of transcription 3 Homo sapiens 22-27 31321245-9 2019 Moreover, carriage of STAT3 "A" risk allele exhibited a higher basal level of STAT3 tyrosine phosphorylation, and an increased IL-23 triggered the pSTAT3 level. Tyrosine 84-92 signal transducer and activator of transcription 3 Homo sapiens 78-83 31058868-4 2019 The two analogues, SG-1709 and SG-1721, inhibited both constitutive as well as inducible STAT3 phosphorylation at tyrosine 705 more effectively than (-)-galiellalactone, which indicates that the analogues are more potent STAT3 blockers. Tyrosine 114-122 signal transducer and activator of transcription 3 Homo sapiens 89-94 31080186-11 2019 Mechanistically, FXT suppressed the IL-6-induced phosphorylation of tyrosine residue (Tyr705) of signal transducer and activator of transcription 3 (STAT3) probably by binding to STAT3. Tyrosine 68-76 signal transducer and activator of transcription 3 Homo sapiens 97-147 31080186-11 2019 Mechanistically, FXT suppressed the IL-6-induced phosphorylation of tyrosine residue (Tyr705) of signal transducer and activator of transcription 3 (STAT3) probably by binding to STAT3. Tyrosine 68-76 signal transducer and activator of transcription 3 Homo sapiens 149-154 31080186-11 2019 Mechanistically, FXT suppressed the IL-6-induced phosphorylation of tyrosine residue (Tyr705) of signal transducer and activator of transcription 3 (STAT3) probably by binding to STAT3. Tyrosine 68-76 signal transducer and activator of transcription 3 Homo sapiens 179-184 30668440-0 2019 Arctiin is a pharmacological inhibitor of STAT3 phosphorylation at tyrosine 705 residue and potentiates bortezomib-induced apoptotic and anti-angiogenic effects in human multiple myeloma cells. Tyrosine 67-75 signal transducer and activator of transcription 3 Homo sapiens 42-47 31068940-5 2019 In particular, we found that CD28-activated RelA/NF-kappaB induces the expression of IL-6 that, in a positive feedback loop, mediates the activation and nuclear translocation of tyrosine phosphorylated STAT3 (pSTAT3). Tyrosine 178-186 signal transducer and activator of transcription 3 Homo sapiens 202-207 30659097-7 2019 Furthermore, HBx stimulated STAT3 phosphorylation at Tyr-705 and promoted the activity and downstream signaling pathway of STAT3 via the SH2D5-TKT interaction. Tyrosine 53-56 signal transducer and activator of transcription 3 Homo sapiens 28-33 30872773-9 2019 JAK2 inhibition led to significantly reduced tyrosine phosphorylation of STAT3 and markedly reduced cystic growth of human and mouse ADPKD-derived cells in cystogenesis assays. Tyrosine 45-53 signal transducer and activator of transcription 3 Homo sapiens 73-78 30668440-4 2019 RESULTS: Arctiin effectively blocked the constitutive activation of STAT3 phosphorylation in the residue of tyrosine 705. Tyrosine 108-116 signal transducer and activator of transcription 3 Homo sapiens 68-73 30251272-5 2018 HFIH selectively suppressed STAT3 phosphorylation at tyrosine 705, not affecting its upstream kinases. Tyrosine 53-61 signal transducer and activator of transcription 3 Homo sapiens 28-33 30631031-7 2019 The phosphorylation status of STAT3 was tested after Ato treatment, showing that Ato mainly stimulated the tyr-705 phosphorylation of STAT3. Tyrosine 107-110 signal transducer and activator of transcription 3 Homo sapiens 30-35 30631031-7 2019 The phosphorylation status of STAT3 was tested after Ato treatment, showing that Ato mainly stimulated the tyr-705 phosphorylation of STAT3. Tyrosine 107-110 signal transducer and activator of transcription 3 Homo sapiens 134-139 30709346-8 2019 The tyrosine phosphorylation of STAT3 was examined by Western Blot analysis. Tyrosine 4-12 signal transducer and activator of transcription 3 Homo sapiens 32-37 30675525-10 2019 Increasing STAT3 amount increases Channel Capacity and robustness, whereas increasing STAT3 tyrosine phosphorylation reduces robustness but increases Channel Capacity. Tyrosine 92-100 signal transducer and activator of transcription 3 Homo sapiens 86-91 30375708-5 2019 HCA inhibited STAT3 phosphorylation at the tyrosine 705 residue, dimer formation, and nuclear translocation in DU145 cells, which led to a downregulation of STAT3 target genes. Tyrosine 43-51 signal transducer and activator of transcription 3 Homo sapiens 14-19 30261753-2 2018 Stimulation by cytokines, growth factors, and hormones triggers this activation by phosphorylating STAT3 at tyrosine 705. Tyrosine 108-116 signal transducer and activator of transcription 3 Homo sapiens 99-104 30261753-5 2018 Further assays showed that the reduction effects of compound 16 on tyrosine 705 of STAT3 were attributed to up-regulation of protein tyrosine phosphatase SHP-1. Tyrosine 67-75 signal transducer and activator of transcription 3 Homo sapiens 83-88 30206377-4 2018 Differentiated HepaRG cells treated with the fatty acid sodium oleate (fatty dHepaRG) recapitulated features of liver vesicular steatosis and activated a cell-autonomous inflammatory response, inducing STAT3-Tyrosine-phosphorylation. Tyrosine 208-216 signal transducer and activator of transcription 3 Homo sapiens 202-207 30455079-8 2018 We previously demonstrated the ability of calcitriol to decrease STAT1 tyrosine 701 (p-STAT1) and STAT3 tyrosine 705 (p-STAT3) phosphorylation as well as inflammatory cytokine output of T cell large granular lymphocyte leukemia cells, but did not determine the effects of calcitriol on NK-LGLL. Tyrosine 104-112 signal transducer and activator of transcription 3 Homo sapiens 98-103 30208623-5 2018 STAT3 activation can be regulated, either positively or negatively, by different posttranslational mechanisms including serine or tyrosine phosphorylation/dephosphorylation, acetylation, or demethylation. Tyrosine 130-138 signal transducer and activator of transcription 3 Homo sapiens 0-5 30208623-6 2018 One of the major mechanisms that negatively regulates STAT3 activation is dephosphorylation of the tyrosine residue essential for its activation by protein tyrosine phosphatases (PTPs). Tyrosine 99-107 signal transducer and activator of transcription 3 Homo sapiens 54-59 29857127-5 2018 Interestingly, FT also suppressed constitutive STAT3 (tyrosine residue 705 and serine residue 727) and STAT5 (tyrosine residue 694/699) activation, which correlated with the suppression of the upstream kinases (JAK1, JAK2, and c-Src) in MM cells, and this effect was found to be mediated via an increased production of reactive oxygen species (ROS) due to GSH/GSSG imbalance. Tyrosine 54-62 signal transducer and activator of transcription 3 Homo sapiens 47-52 29488000-0 2018 Bilateral activation of STAT3 by phosphorylation at the tyrosine-705 (Y705) and serine-727 (S727) positions and its nuclear translocation in primary sensory neurons following unilateral sciatic nerve injury. Tyrosine 56-64 signal transducer and activator of transcription 3 Homo sapiens 24-29 30197757-7 2018 Recombinant IL-11-induced phosphorylation of signal transducer and activator of transcription 3 at tyrosine 705 was reduced in a dose-dependent manner in HeLa cells. Tyrosine 99-107 signal transducer and activator of transcription 3 Homo sapiens 45-95 30135654-3 2018 Lycorine selectively inactivates phospho-STAT3 (Tyr-705), and subsequent molecular docking uncovers that lycorine directly binds to the SH2 domain of STAT3. Tyrosine 48-51 signal transducer and activator of transcription 3 Homo sapiens 41-46 30135654-3 2018 Lycorine selectively inactivates phospho-STAT3 (Tyr-705), and subsequent molecular docking uncovers that lycorine directly binds to the SH2 domain of STAT3. Tyrosine 48-51 signal transducer and activator of transcription 3 Homo sapiens 150-155 30043217-8 2018 Cantharidin inhibited STAT3 tyrosine phosphorylation in MDA-MB-231 cells. Tyrosine 28-36 signal transducer and activator of transcription 3 Homo sapiens 22-27 30127519-0 2018 The tyrosine phosphorylated pro-survival form of Fas intensifies the EGF-induced signal in colorectal cancer cells through the nuclear EGFR/STAT3-mediated pathway. Tyrosine 4-12 signal transducer and activator of transcription 3 Homo sapiens 140-145 29807795-4 2018 CG-06 inhibited STAT3 phosphorylation at tyrosine 705 in a dose- and time dependent manner in DU-145 cells and suppressed IL-6-induced STAT3 phosphorylation at Tyr-705 in DU-145 and LNCaP cell lines. Tyrosine 41-49 signal transducer and activator of transcription 3 Homo sapiens 16-21 29807795-4 2018 CG-06 inhibited STAT3 phosphorylation at tyrosine 705 in a dose- and time dependent manner in DU-145 cells and suppressed IL-6-induced STAT3 phosphorylation at Tyr-705 in DU-145 and LNCaP cell lines. Tyrosine 160-163 signal transducer and activator of transcription 3 Homo sapiens 135-140 29488000-2 2018 STAT3 activation was by phosphorylation at the tyrosine-705 (Y705) and serine-727 (S727) positions and was followed by their nuclear translocation. Tyrosine 47-55 signal transducer and activator of transcription 3 Homo sapiens 0-5 29861864-8 2018 On the human phospho-kinase array analysis of 46 specific Ser/Thr or Tyr phosphorylation sites on 39 selected proteins, eNOS, AKT1/2/3, RSK1/2/3 and STAT3 phosphorylation were decreased by FTY-720 treatment. Tyrosine 69-72 signal transducer and activator of transcription 3 Homo sapiens 149-154 29555504-8 2018 ALA inhibited tyrosine phosphorylation (Tyr705pho) of STAT3 to down-regulate APOC3 expression. Tyrosine 14-22 signal transducer and activator of transcription 3 Homo sapiens 54-59 29660364-2 2018 Silibinin reduced IL-6 inducible, constitutive, and acquired feedback activation of STAT3 at tyrosine 705 (Y705). Tyrosine 93-101 signal transducer and activator of transcription 3 Homo sapiens 84-89 29765448-7 2018 However, the STAT3 phosphorylation (Tyr 705) was attenuated by sustained hypoxia (p < 0.01). Tyrosine 36-39 signal transducer and activator of transcription 3 Homo sapiens 13-18 29774125-6 2018 Moreover, we found that STAT3 was constitutively activated in GICs by phosphorylation on both tyrosine (Y705) and serine (S727) residues. Tyrosine 94-102 signal transducer and activator of transcription 3 Homo sapiens 24-29 29765448-8 2018 Moreover, it was demonstrated that HO-1 overexpression significantly inhibited the hypoxia-promoted STAT3 phosphorylation (Tyr 705). Tyrosine 123-126 signal transducer and activator of transcription 3 Homo sapiens 100-105 29765448-9 2018 Conclusions: HO-1 was overexpressed in PE placenta, in association with reduced STAT3 phosphorylation (Tyr 705). Tyrosine 103-106 signal transducer and activator of transcription 3 Homo sapiens 80-85 29765448-11 2018 Thus, we speculate that overexpressed HO-1 might contribute to the reduced STAT3 phosphorylation (Tyr 705) and the pathogenesis of preeclampsia. Tyrosine 98-101 signal transducer and activator of transcription 3 Homo sapiens 75-80 28558479-3 2018 Recent Advances: Extracellular IL-6, which stimulates the release of cortisol from the zona fasciculata of the adrenal cortex, mediates its intracellular effects by tyrosine phosphorylation of the transcription factor signal transducer and activator of transcription 3 (STAT3). Tyrosine 165-173 signal transducer and activator of transcription 3 Homo sapiens 218-268 29873274-4 2018 The dogma of STAT3 activity posits that it is a tyrosine phosphorylated transcription factor which modulates the expression of hundreds of genes. Tyrosine 48-56 signal transducer and activator of transcription 3 Homo sapiens 13-18 29873274-6 2018 In addition to tyrosine phosphorylation, STAT3 is decorated with a plethora of post-translational modifications which regulate STAT3"s nuclear function in addition to its non-genomic activities. Tyrosine 15-23 signal transducer and activator of transcription 3 Homo sapiens 41-46 28558479-3 2018 Recent Advances: Extracellular IL-6, which stimulates the release of cortisol from the zona fasciculata of the adrenal cortex, mediates its intracellular effects by tyrosine phosphorylation of the transcription factor signal transducer and activator of transcription 3 (STAT3). Tyrosine 165-173 signal transducer and activator of transcription 3 Homo sapiens 270-275 29431732-5 2018 Specifically, blocking AhR redirected IFN-beta signaling to STAT3 phosphorylation through both tyrosine and serine sites, which subsequently facilitated STAT3 nuclear translocation and subsequent binding to the p53 promoter in the nucleus. Tyrosine 95-103 signal transducer and activator of transcription 3 Homo sapiens 60-65 29456111-2 2018 Methyllucidone inhibited STAT3 phosphorylation at tyrosine 705 in a dose- and time dependent manner in DU145 prostate cancer cells and suppressed IL-6-induced STAT3 phosphorylation at Tyr-705 in LNCaP cells. Tyrosine 50-58 signal transducer and activator of transcription 3 Homo sapiens 25-30 29456111-2 2018 Methyllucidone inhibited STAT3 phosphorylation at tyrosine 705 in a dose- and time dependent manner in DU145 prostate cancer cells and suppressed IL-6-induced STAT3 phosphorylation at Tyr-705 in LNCaP cells. Tyrosine 184-187 signal transducer and activator of transcription 3 Homo sapiens 159-164 29431732-5 2018 Specifically, blocking AhR redirected IFN-beta signaling to STAT3 phosphorylation through both tyrosine and serine sites, which subsequently facilitated STAT3 nuclear translocation and subsequent binding to the p53 promoter in the nucleus. Tyrosine 95-103 signal transducer and activator of transcription 3 Homo sapiens 153-158 29207010-8 2018 Finally, the present study indicated that miR-19b may suppress signal transducer and activator of transcription 3 (STAT3) tyrosine phosphorylation and transcriptional activity in ECs, as determined by western blot analysis and luciferase reporter assay. Tyrosine 122-130 signal transducer and activator of transcription 3 Homo sapiens 63-113 29402895-2 2018 Signal transducer and activator of transcription 3 (STAT3) dimerizes after tyrosine phosphorylation upon cytokine stimulation. Tyrosine 75-83 signal transducer and activator of transcription 3 Homo sapiens 0-50 29402895-2 2018 Signal transducer and activator of transcription 3 (STAT3) dimerizes after tyrosine phosphorylation upon cytokine stimulation. Tyrosine 75-83 signal transducer and activator of transcription 3 Homo sapiens 52-57 29207010-8 2018 Finally, the present study indicated that miR-19b may suppress signal transducer and activator of transcription 3 (STAT3) tyrosine phosphorylation and transcriptional activity in ECs, as determined by western blot analysis and luciferase reporter assay. Tyrosine 122-130 signal transducer and activator of transcription 3 Homo sapiens 115-120 28881473-6 2018 In cultured podocyte, high glucose (HG) up-regulates the expression of mIL-6R and gp130, as well as STAT3 tyrosine 705 phosphorylation, in a time-dependent manner. Tyrosine 106-114 signal transducer and activator of transcription 3 Homo sapiens 100-105 28497593-11 2018 Biochemical analyses indicated that PTPRD rs35929428 promoted dephosphorylation of tyrosine 705 signal transducer and activator of transcription 3 (Tyr 705) in hepatocytes. Tyrosine 83-91 signal transducer and activator of transcription 3 Homo sapiens 96-146 28497593-11 2018 Biochemical analyses indicated that PTPRD rs35929428 promoted dephosphorylation of tyrosine 705 signal transducer and activator of transcription 3 (Tyr 705) in hepatocytes. Tyrosine 148-151 signal transducer and activator of transcription 3 Homo sapiens 96-146 28497593-13 2018 Through exacerbation of the dephosphorylation of signal transducer and activator of transcription 3 (Tyr 705) in hepatocytes, PTPRD rs35929428 might play a role in hepatic lipid accumulation and fibrosis, followed by the development of NAFLD. Tyrosine 101-104 signal transducer and activator of transcription 3 Homo sapiens 49-99 29234062-5 2017 Erasin inhibits tyrosine phosphorylation of STAT3 with selectivity over STAT5 and STAT1 in cell-based assays, and increases the apoptotic rate of cultured NSCLC cells in a STAT3-dependent manner. Tyrosine 16-24 signal transducer and activator of transcription 3 Homo sapiens 44-49 29162862-5 2017 We here show that a second tyrosine phosphorylation within the SH2 domain at position Y640, induced by Tyk2, negatively controls STAT3 activity. Tyrosine 27-35 signal transducer and activator of transcription 3 Homo sapiens 129-134 28783167-3 2017 TSN selectively inactivates phospho-STAT3 (Tyr-705); subsequent molecular docking and in vitro SPR analysis uncover TSN directly binds to the SH2 domain of STAT3. Tyrosine 43-46 signal transducer and activator of transcription 3 Homo sapiens 36-41 28783167-3 2017 TSN selectively inactivates phospho-STAT3 (Tyr-705); subsequent molecular docking and in vitro SPR analysis uncover TSN directly binds to the SH2 domain of STAT3. Tyrosine 43-46 signal transducer and activator of transcription 3 Homo sapiens 156-161 29126425-10 2017 Mechanistic study revealed that Hdac7 mutation in mouse lung tumors or HDAC7 depletion in human tumor cell lines resulted in significantly enhanced acetylation and tyrosine-phosphorylation of Stat3 and HDAC7 protein directly interacted with and deacetylateed STAT3. Tyrosine 164-172 signal transducer and activator of transcription 3 Homo sapiens 192-197 28722170-1 2017 In chronic lymphocytic leukemia (CLL) cells, both interleukin-6 (IL-6) and the B-cell receptor (BCR) activate Janus kinase 2 (JAK2) and induce the phosphorylation of signal transduction and activator of transcription 3 (STAT3) on tyrosine 705 residues. Tyrosine 230-238 signal transducer and activator of transcription 3 Homo sapiens 166-218 28722170-8 2017 The IgM-induced production of IL-6 prompted the phosphorylation of STAT3 on tyrosine residues. Tyrosine 76-84 signal transducer and activator of transcription 3 Homo sapiens 67-72 29126425-10 2017 Mechanistic study revealed that Hdac7 mutation in mouse lung tumors or HDAC7 depletion in human tumor cell lines resulted in significantly enhanced acetylation and tyrosine-phosphorylation of Stat3 and HDAC7 protein directly interacted with and deacetylateed STAT3. Tyrosine 164-172 signal transducer and activator of transcription 3 Homo sapiens 259-264 28090628-3 2017 Immunohistochemical assessment of the phosphorylated levels of STAT3(tyrosine/ serine), ERK1/2 and JNK was performed in 60 OSCC, including well, moderately and poorly differentiated tumors. Tyrosine 69-77 signal transducer and activator of transcription 3 Homo sapiens 63-68 28739485-6 2017 We further show that ACK1 promotes the phosphorylation and nuclear accumulation of STAT3 in cultured cells and that the levels of ACK1 correlate positively with the levels of tyrosine phosphorylated STAT3 in primary lung adenocarcinoma (ADC) cells. Tyrosine 175-183 signal transducer and activator of transcription 3 Homo sapiens 83-88 28739485-6 2017 We further show that ACK1 promotes the phosphorylation and nuclear accumulation of STAT3 in cultured cells and that the levels of ACK1 correlate positively with the levels of tyrosine phosphorylated STAT3 in primary lung adenocarcinoma (ADC) cells. Tyrosine 175-183 signal transducer and activator of transcription 3 Homo sapiens 199-204 28634059-4 2017 Such inhibition is found to be achieved through down-regulation of constitutive phosphorylation of JAK2, but not JAk1, c-Src, ERK1/2, and Akt, which means STAT3 tyrosine phosphorylation in HepG2 cells following PPPF treatment is associated with a reduction in JAK2 activity. Tyrosine 161-169 signal transducer and activator of transcription 3 Homo sapiens 155-160 28666623-3 2017 CG902 specifically inhibited STAT3 phosphorylation at Tyr-705 in DU145 prostate cancer cells and decreased the expression levels of STAT3 target genes, such as cyclin D1, cyclin A, and survivin. Tyrosine 54-57 signal transducer and activator of transcription 3 Homo sapiens 29-34 28090628-10 2017 ERK1/2 and STAT3 activation (as assessed by tyrosine but not serine phosphorylation) could contribute to a less differentiated phenotype in OSCC, while JNK activation may have an opposite, although possibly less pronounced, effect. Tyrosine 44-52 signal transducer and activator of transcription 3 Homo sapiens 11-16 28856117-11 2017 Low concentrations of MH (0.03-1.25% w/v) induced a rapid reduction in tyrosine-phosphorylated STAT3 (pY-STAT3) in MDA-MB-231 and MCF-7 cells. Tyrosine 71-79 signal transducer and activator of transcription 3 Homo sapiens 95-100 27660268-5 2017 This review focuses on the role of serine 727 and tyrosine 705 phosphorylation of STAT3 in glioma. Tyrosine 50-58 signal transducer and activator of transcription 3 Homo sapiens 82-87 28729053-5 2017 Furthermore, treatment of U251MG with 6 and 7 inhibited intracellular STAT3 tyrosine phosphorylation and glioma cell migration in vitro, respectively. Tyrosine 76-84 signal transducer and activator of transcription 3 Homo sapiens 70-75 28856117-11 2017 Low concentrations of MH (0.03-1.25% w/v) induced a rapid reduction in tyrosine-phosphorylated STAT3 (pY-STAT3) in MDA-MB-231 and MCF-7 cells. Tyrosine 71-79 signal transducer and activator of transcription 3 Homo sapiens 105-110 28520923-6 2017 Finally, we demonstrate that the supernatants derived from HOXA10-depleted decidual cell-phosphorylated STAT3 (Tyr 705) and knocking down STAT3 in ACH-3P and JEG3 cells restrained the invasion mediated by supernatants derived from HOXA10-depleted decidual cells. Tyrosine 111-114 signal transducer and activator of transcription 3 Homo sapiens 104-109 28750624-9 2017 In addition, leptin stimulated the phosphorylation of signal transducer and activator of transcription-3 (STAT3) Tyr-705 as well as estrogen receptor (ER)alpha, and the expression of ERalpha-responsive genes. Tyrosine 113-116 signal transducer and activator of transcription 3 Homo sapiens 54-104 28468777-9 2017 STAT3/STAT5 activation depends on tyrosine phosphorylation, mediated by several upstream TKs. Tyrosine 34-42 signal transducer and activator of transcription 3 Homo sapiens 0-5 28486761-8 2017 Panitumumab and the tyrosine kinase inhibitor erlotinib reduced the basal level of EGFR tyrosine phosphorylation and reversed FTD-induced ERK/AKT/STAT3 and EGFR serine/threonine phosphorylation. Tyrosine 20-28 signal transducer and activator of transcription 3 Homo sapiens 146-151 28750624-9 2017 In addition, leptin stimulated the phosphorylation of signal transducer and activator of transcription-3 (STAT3) Tyr-705 as well as estrogen receptor (ER)alpha, and the expression of ERalpha-responsive genes. Tyrosine 113-116 signal transducer and activator of transcription 3 Homo sapiens 106-111 28542650-6 2017 In addition, significant phosphorylation of STAT1 (ser 727) and STAT3 (both tyr 705 and ser 727 residues) was also observed, accompanied by a decrease in total STAT1. Tyrosine 76-79 signal transducer and activator of transcription 3 Homo sapiens 64-69 28521301-5 2017 However, afatinib therapy causes phosphorylation of STAT3 tyrosine 705 (pSTAT3Tyr705) and elevation of STAT3 and RANTES mRNA levels. Tyrosine 58-66 signal transducer and activator of transcription 3 Homo sapiens 52-57 28521301-5 2017 However, afatinib therapy causes phosphorylation of STAT3 tyrosine 705 (pSTAT3Tyr705) and elevation of STAT3 and RANTES mRNA levels. Tyrosine 58-66 signal transducer and activator of transcription 3 Homo sapiens 73-78 28228214-6 2017 Phosphorylation levels of signal transducer and activator of transcription 3 (STAT3) at Tyr-705, a crucial site for STAT3 activation and target site of PTPN2, decreased upon exposure to the inhibitor. Tyrosine 88-91 signal transducer and activator of transcription 3 Homo sapiens 26-76 28228214-6 2017 Phosphorylation levels of signal transducer and activator of transcription 3 (STAT3) at Tyr-705, a crucial site for STAT3 activation and target site of PTPN2, decreased upon exposure to the inhibitor. Tyrosine 88-91 signal transducer and activator of transcription 3 Homo sapiens 78-83 28228214-6 2017 Phosphorylation levels of signal transducer and activator of transcription 3 (STAT3) at Tyr-705, a crucial site for STAT3 activation and target site of PTPN2, decreased upon exposure to the inhibitor. Tyrosine 88-91 signal transducer and activator of transcription 3 Homo sapiens 116-121 27502766-3 2016 Previous studies have shown that knocking down SOCS3 in spinal cord neurons with Lentiviral delivery of SOCS3-targeting shRNA (shSOCS3) increased spinal cord injury (SCI)-induced tyrosine phosphorylation of STAT3 (P-STAT3 Tyr), which in part contributed to decreased neuronal death and demyelination as well as enhanced dendritic regeneration and protection of neuronal morphology after SCI. Tyrosine 179-187 signal transducer and activator of transcription 3 Homo sapiens 207-212 28108315-6 2017 Tyrosine-phosphorylated STAT3 protein is known as a crucial signaling molecule in the regulation of tumorigenesis and metastasis. Tyrosine 0-8 signal transducer and activator of transcription 3 Homo sapiens 24-29 28351946-1 2017 Signal transducer and activator of transcription 3 (STAT3) is associated with various physiological and pathological functions, mainly as a transcription factor that translocates to the nucleus upon tyrosine phosphorylation induced by cytokine stimulation. Tyrosine 199-207 signal transducer and activator of transcription 3 Homo sapiens 0-50 28351946-1 2017 Signal transducer and activator of transcription 3 (STAT3) is associated with various physiological and pathological functions, mainly as a transcription factor that translocates to the nucleus upon tyrosine phosphorylation induced by cytokine stimulation. Tyrosine 199-207 signal transducer and activator of transcription 3 Homo sapiens 52-57 28351319-5 2017 In addition, splicing factor 3b subunit 4 was found to inhibit the activity of signal transducer and activator of transcription 3 signaling via downregulating the phosphorylation of signal transducer and activator of transcription 3 on a tyrosine residue at position 705. Tyrosine 238-246 signal transducer and activator of transcription 3 Homo sapiens 79-129 28351319-5 2017 In addition, splicing factor 3b subunit 4 was found to inhibit the activity of signal transducer and activator of transcription 3 signaling via downregulating the phosphorylation of signal transducer and activator of transcription 3 on a tyrosine residue at position 705. Tyrosine 238-246 signal transducer and activator of transcription 3 Homo sapiens 182-232 27709605-2 2017 STAT3 also down-regulates the production of pro-inflammatory cytokines induced by immunoreceptor tyrosine-based activation motif (ITAM)-coupled receptors, a mechanism termed cross-inhibition. Tyrosine 97-105 signal transducer and activator of transcription 3 Homo sapiens 0-5 26705936-8 2017 However, only tyrosine phosphorylated STAT3 was detected in the nucleus, which induced upregulation of Bcl-2 and downregulation of Bax and Bak. Tyrosine 14-22 signal transducer and activator of transcription 3 Homo sapiens 38-43 26705936-10 2017 Thus, we present evidence for the role of M2 phenotype TAM in IL-6 receptor-mediated signals, particularly tyrosine phosphorylation of STAT3, responsible for the prosurvival adaptation of tumor cells to hypoxia. Tyrosine 107-115 signal transducer and activator of transcription 3 Homo sapiens 135-140 27938379-1 2016 BACKGROUND: STAT3 signaling plays the pivotal role in tumorigenesis through EZH2 epigenetic modification, which enhanced STAT3 activity by increased tyrosine phosphorylation of STAT3. Tyrosine 149-157 signal transducer and activator of transcription 3 Homo sapiens 12-17 27938379-1 2016 BACKGROUND: STAT3 signaling plays the pivotal role in tumorigenesis through EZH2 epigenetic modification, which enhanced STAT3 activity by increased tyrosine phosphorylation of STAT3. Tyrosine 149-157 signal transducer and activator of transcription 3 Homo sapiens 121-126 27938379-1 2016 BACKGROUND: STAT3 signaling plays the pivotal role in tumorigenesis through EZH2 epigenetic modification, which enhanced STAT3 activity by increased tyrosine phosphorylation of STAT3. Tyrosine 149-157 signal transducer and activator of transcription 3 Homo sapiens 121-126 27855189-1 2016 BACKGROUND: Hyperactivation of STAT3 via constitutive phosphorylation of tyrosine 705 (Y705) is common in most human cancers, including head and neck squamous carcinoma (HNSCC). Tyrosine 73-81 signal transducer and activator of transcription 3 Homo sapiens 31-36 27739031-4 2016 Expression of vascular endothelial growth factor (VEGF) and tyrosine-phosphorylated signal transducer and activator of transcription 3 (p-STAT3) was monitored in the experimental groups using a qPCR assay and western blot analysis. Tyrosine 60-68 signal transducer and activator of transcription 3 Homo sapiens 138-143 27486258-1 2016 Signal transducer and activator of transcription 3 (STAT3) is a transcription factor activated by the phosphorylation of tyrosine 705 in response to many cytokines and growth factors. Tyrosine 121-129 signal transducer and activator of transcription 3 Homo sapiens 0-50 27486258-1 2016 Signal transducer and activator of transcription 3 (STAT3) is a transcription factor activated by the phosphorylation of tyrosine 705 in response to many cytokines and growth factors. Tyrosine 121-129 signal transducer and activator of transcription 3 Homo sapiens 52-57 27862996-8 2017 However, TA binds to EGF-R and inhibits the tyrosine phosphorylation of both STAT1 and STAT3. Tyrosine 44-52 signal transducer and activator of transcription 3 Homo sapiens 87-92 26649644-2 2016 Canonical Stat3-mediated signaling involves tyrosine phosphorylation on specific residues that leads to homodimerization and translocation to the nucleus. Tyrosine 44-52 signal transducer and activator of transcription 3 Homo sapiens 10-15 27502766-3 2016 Previous studies have shown that knocking down SOCS3 in spinal cord neurons with Lentiviral delivery of SOCS3-targeting shRNA (shSOCS3) increased spinal cord injury (SCI)-induced tyrosine phosphorylation of STAT3 (P-STAT3 Tyr), which in part contributed to decreased neuronal death and demyelination as well as enhanced dendritic regeneration and protection of neuronal morphology after SCI. Tyrosine 222-225 signal transducer and activator of transcription 3 Homo sapiens 207-212 27318793-2 2016 In this study, we demonstrate that signal transducer and activator of transcription 3 (STAT3) is phosphorylated on its conserved tyrosine residue (Y705) following RVFV infection. Tyrosine 129-137 signal transducer and activator of transcription 3 Homo sapiens 87-92 27373978-9 2016 Importantly, we show that GV treatment decreases STAT3 phosphorylation at residue tyrosine 705, thus preventing the translocation of STAT3 into the nucleus and its binding to SOX2 promoter. Tyrosine 82-90 signal transducer and activator of transcription 3 Homo sapiens 49-54 27486189-5 2016 STAT3 is activated by tyrosine phosphorylation in response to many cytokines and can orchestrate effector responses that include proliferation, inflammation, metastasis, and developmental programming. Tyrosine 22-30 signal transducer and activator of transcription 3 Homo sapiens 0-5 27648129-7 2016 Then the Pearson correlation analysis using serial tissue sections showed a close correlation of PAK2 with Cyclin D1, Phospho-STAT3 at Tyrosine 705 (p-STAT3) and Ki-67. Tyrosine 135-143 signal transducer and activator of transcription 3 Homo sapiens 126-131 27040695-2 2016 Cytokine-induced gene transcription greatly depends on tyrosine phosphorylation of STAT3 on a single tyrosine residue with subsequent nuclear accumulation and specific DNA sequence (GAS) recognition. Tyrosine 55-63 signal transducer and activator of transcription 3 Homo sapiens 83-88 27040695-2 2016 Cytokine-induced gene transcription greatly depends on tyrosine phosphorylation of STAT3 on a single tyrosine residue with subsequent nuclear accumulation and specific DNA sequence (GAS) recognition. Tyrosine 101-109 signal transducer and activator of transcription 3 Homo sapiens 83-88 27347203-2 2016 Persistent activation of signal transducer and activator of transcription 3 (STAT3), which is accompanied by increases in STAT3 tyrosine phosphorylation, is associated with cell proliferation, differentiation and apoptosis in oral squamous cell carcinoma (OSCC). Tyrosine 128-136 signal transducer and activator of transcription 3 Homo sapiens 25-75 27259262-1 2016 Constitutive STAT3 activation by tyrosine phosphorylation of mutated or amplified tyrosine kinases (pYSTAT3) is critical for cancer initiation, progression, invasion, and motility of carcinoma cells. Tyrosine 33-41 signal transducer and activator of transcription 3 Homo sapiens 13-18 27347203-2 2016 Persistent activation of signal transducer and activator of transcription 3 (STAT3), which is accompanied by increases in STAT3 tyrosine phosphorylation, is associated with cell proliferation, differentiation and apoptosis in oral squamous cell carcinoma (OSCC). Tyrosine 128-136 signal transducer and activator of transcription 3 Homo sapiens 77-82 27347203-2 2016 Persistent activation of signal transducer and activator of transcription 3 (STAT3), which is accompanied by increases in STAT3 tyrosine phosphorylation, is associated with cell proliferation, differentiation and apoptosis in oral squamous cell carcinoma (OSCC). Tyrosine 128-136 signal transducer and activator of transcription 3 Homo sapiens 122-127 26927669-3 2016 Specifically, we determined that UT2 interacts directly with GP130 and inhibits phosphorylation of STAT3 on tyrosine 705 (STAT3Y705). Tyrosine 108-116 signal transducer and activator of transcription 3 Homo sapiens 99-104 26976343-8 2016 Taxol exposure induced high levels of phospho-STAT3 (Tyr 705) and TXNDC17; and increase of basal autophagy in colorectal cancer cells. Tyrosine 53-56 signal transducer and activator of transcription 3 Homo sapiens 46-51 26976343-11 2016 And it has been proved that phospho-STAT3 (Tyr 705) possesses transcriptional regulation activity through forming dimmers. Tyrosine 43-46 signal transducer and activator of transcription 3 Homo sapiens 36-41 27064016-6 2016 RESULTS: We found that HN3 and FaDu cells expressed strongly phosphorylated STAT3 on both tyrosine 705 and serine 727 residues as compared to other SCCHN cells. Tyrosine 90-98 signal transducer and activator of transcription 3 Homo sapiens 76-81 26669927-7 2016 Systemic pre-insult STAT3-blockade at tyrosine 705 (Y705) with JAK2-inhibitor WP1066 reduced microglial and astroglial activation to a more moderate degree, but in a pattern similar to the one produced by the cell-specific deletions. Tyrosine 38-46 signal transducer and activator of transcription 3 Homo sapiens 20-25 26904942-7 2016 Loss of Ezh2 also resulted in increased activation of STAT3 by tyrosine 705 phosphorylation. Tyrosine 63-71 signal transducer and activator of transcription 3 Homo sapiens 54-59 26898989-6 2016 The phosphorylation of tyrosine-705 of STAT3 is the first step in OLA1P2 binding, and the formation of phosphorylated STAT3 homodimers is subsequently blocked. Tyrosine 23-31 signal transducer and activator of transcription 3 Homo sapiens 39-44 26646273-4 2016 RESULTS: Morusin specifically inhibited constitutive STAT3 activation both at tyrosine residue 705 and serine residue 727 in 4 pancreatic tumor cells. Tyrosine 78-86 signal transducer and activator of transcription 3 Homo sapiens 53-58 30695496-3 2016 This leads to activation of tyrosine kinase JAK2, which phosphory- lates tyrosine-containing sites located in the cytoplasmic domain of the receptor, resulting in stimulation of activity of phosphatidylinositol-3-kinase, the transcription factors STAT3 and STAT5, phosphatase SHP2, and mitogen-activated protein kinase. Tyrosine 28-36 signal transducer and activator of transcription 3 Homo sapiens 247-252 26898989-6 2016 The phosphorylation of tyrosine-705 of STAT3 is the first step in OLA1P2 binding, and the formation of phosphorylated STAT3 homodimers is subsequently blocked. Tyrosine 23-31 signal transducer and activator of transcription 3 Homo sapiens 118-123 25736961-5 2016 Here, we studied the role of STAT3 activation on tyrosine 705 (Y705) and serine 727 (S727) in glioma radioresistance. Tyrosine 49-57 signal transducer and activator of transcription 3 Homo sapiens 29-34 26893580-7 2016 RESULTS: In eight of nine ERalpha-positive breast cancer cell lines, recombinant IL-6 increased phosphorylation of tyrosine 705 of STAT3. Tyrosine 115-123 signal transducer and activator of transcription 3 Homo sapiens 131-136 27274723-2 2016 Results demonstrated that both Anxa 2 and STAT3 were highly expressed in CRC specimens in both mRNA and protein levels, with or without phosphorylation (Tyrosine 23 in Anxa 2 and Tyrosine 705 in STAT3). Tyrosine 153-161 signal transducer and activator of transcription 3 Homo sapiens 42-47 27274723-3 2016 And the upregulated Anxa 2 promoted the phosphorylation of STAT3 (Tyrosine 705) in CRC Caco-2 cells. Tyrosine 66-74 signal transducer and activator of transcription 3 Homo sapiens 59-64 26553978-1 2015 Crystallography of the cores of phosphotyrosine-activated dimers of STAT1 (132-713) and STAT3 (127-722) bound to a similar double-stranded deoxyoligonucleotide established the domain structure of the STATs and the structural basis for activation through tyrosine phosphorylation and dimerization. Tyrosine 39-47 signal transducer and activator of transcription 3 Homo sapiens 88-93 26549437-5 2016 Surprisingly, pimozide reduced the basal expression of phosphorylation STAT3 at tyrosine 705 and reversed the expression of phosphorylation of STAT3 induced by IL-6 addition, suggesting that pimozide can suppress cellular STAT3 activation. Tyrosine 80-88 signal transducer and activator of transcription 3 Homo sapiens 71-76 26045618-2 2015 When phosphorylated at tyrosine 705 (Tyr705), STAT3 functions as a nuclear transcription factor that can contribute to oncogenesis. Tyrosine 23-31 signal transducer and activator of transcription 3 Homo sapiens 46-51 25619838-6 2015 Depletion of SHP-1 could induce a more increase in TGF-beta1-induced p-STAT3(Tyr-705) and EMT characteristics, further supporting the mechanism that suppression of TGF-beta1-induced EMT is dependent on SHP-1-mediated STAT3 inactivation. Tyrosine 77-80 signal transducer and activator of transcription 3 Homo sapiens 71-76 26212545-4 2015 Sugiol specifically inhibited STAT3 phosphorylation at Tyr-705 in DU145 prostate cells, and this inhibition was independent of the STAT3 upstream kinase. Tyrosine 55-58 signal transducer and activator of transcription 3 Homo sapiens 30-35 26245900-9 2015 Furthermore, mutant KRAS activated p-STAT3 (Tyr(705)) in the absence of IL-6 secretion, and STAT3 knockdown reduced BCL-XL mRNA and protein expression. Tyrosine 44-47 signal transducer and activator of transcription 3 Homo sapiens 37-42 26384335-5 2015 In parallel to SOCS3 expression, IL-6 induced tyrosine phosphorylation of signal transducer and activator of transcription 3 (STAT3) in NS-1 cells. Tyrosine 46-54 signal transducer and activator of transcription 3 Homo sapiens 74-124 26384335-5 2015 In parallel to SOCS3 expression, IL-6 induced tyrosine phosphorylation of signal transducer and activator of transcription 3 (STAT3) in NS-1 cells. Tyrosine 46-54 signal transducer and activator of transcription 3 Homo sapiens 126-131 26384335-6 2015 Lentiviral delivery of short hairpin RNA (shSOCS3) (Lenti-shSOCS3) to decrease SOCS3 expression into NS-1 cells enhanced IL-6-induced tyrosine phosphorylation of STAT3 (P-STAT3 Tyr705) and promoted neurite outgrowth. Tyrosine 134-142 signal transducer and activator of transcription 3 Homo sapiens 162-167 26231159-3 2015 Stimulation with IFNalpha/beta leads to the tyrosine phosphorylation of the JAK1 and Tyk2 receptor-associated kinases with subsequent STATs activation, transmitting signals from the cell surface receptor to the nucleus. Tyrosine 44-52 signal transducer and activator of transcription 3 Homo sapiens 134-139 26234940-2 2015 Signal transducer and activator of transcription 3 protein (STAT3), a transcription factor that is persistently tyrosine-705 phosphorylated (pSTAT3) in response to numerous oncogenic signaling pathways, activates downstream proliferative and anti-apoptotic pathways. Tyrosine 112-120 signal transducer and activator of transcription 3 Homo sapiens 0-50 26234940-2 2015 Signal transducer and activator of transcription 3 protein (STAT3), a transcription factor that is persistently tyrosine-705 phosphorylated (pSTAT3) in response to numerous oncogenic signaling pathways, activates downstream proliferative and anti-apoptotic pathways. Tyrosine 112-120 signal transducer and activator of transcription 3 Homo sapiens 60-65 26045618-7 2015 Introduction of a STAT3CA construct with a mutated tyrosine phosphorylation site into H-Ras(G12V)-expressing Barrett"s cells resulted in high levels of mitochondrial phospho-STAT3 (Ser727) with little or no nuclear phospho-STAT3 (Tyr705), and the cells still formed tumors in immunodeficient mice. Tyrosine 51-59 signal transducer and activator of transcription 3 Homo sapiens 18-25 26045618-7 2015 Introduction of a STAT3CA construct with a mutated tyrosine phosphorylation site into H-Ras(G12V)-expressing Barrett"s cells resulted in high levels of mitochondrial phospho-STAT3 (Ser727) with little or no nuclear phospho-STAT3 (Tyr705), and the cells still formed tumors in immunodeficient mice. Tyrosine 51-59 signal transducer and activator of transcription 3 Homo sapiens 18-23 26045618-7 2015 Introduction of a STAT3CA construct with a mutated tyrosine phosphorylation site into H-Ras(G12V)-expressing Barrett"s cells resulted in high levels of mitochondrial phospho-STAT3 (Ser727) with little or no nuclear phospho-STAT3 (Tyr705), and the cells still formed tumors in immunodeficient mice. Tyrosine 51-59 signal transducer and activator of transcription 3 Homo sapiens 174-179 25962106-8 2015 Wogonin directly inhibited the activation of STAT3 on tyrosine 705 in tumor cells. Tyrosine 54-62 signal transducer and activator of transcription 3 Homo sapiens 45-50 26017975-3 2015 Exposure of podocytes to TNF increased phosphorylation of NF-kappaB p65-RelA followed by increased tyrosine phosphorylation of STAT3. Tyrosine 99-107 signal transducer and activator of transcription 3 Homo sapiens 127-132 26166037-9 2015 HHT reversiblely inhibited IL-6-induced STAT3 Tyrosine 705 phosphorylation and reduced anti-apoptotic proteins expression. Tyrosine 46-54 signal transducer and activator of transcription 3 Homo sapiens 40-45 24793791-3 2015 In the present study, we first demonstrate that STAT3 phosphorylation at tyrosine 705 is accompanied by a concomitant increase in the expression level of NPM. Tyrosine 73-81 signal transducer and activator of transcription 3 Homo sapiens 48-53 25883212-6 2015 In this study, we hypothesized LLL12 inhibits STAT3 phosphorylation at tyrosine 705 (Y705) in HCC and show antitumor activity in HCC in vitro and in vivo. Tyrosine 71-79 signal transducer and activator of transcription 3 Homo sapiens 46-51 25926075-5 2015 MMP-1 and tyrosine-phosphorylated activated STAT3 were found co-localized in cancer tissues, most pronounced in tumor fronts, and in particular in adenocarcinomas. Tyrosine 10-18 signal transducer and activator of transcription 3 Homo sapiens 44-49 25609240-10 2015 MAIN RESULTS AND THE ROLE OF CHANCE: Placental P-STAT3 (Tyr-705) was increased in women with PCOS (P < 0.05) versus controls. Tyrosine 56-59 signal transducer and activator of transcription 3 Homo sapiens 49-54 25704089-6 2015 Furthermore, AZD1480 inhibited the expression of phosphorylated-(Tyr-705)-signal transducer and activator of transcription 3 (STAT3) and La in Huh7 cells. Tyrosine 65-68 signal transducer and activator of transcription 3 Homo sapiens 74-124 25704089-6 2015 Furthermore, AZD1480 inhibited the expression of phosphorylated-(Tyr-705)-signal transducer and activator of transcription 3 (STAT3) and La in Huh7 cells. Tyrosine 65-68 signal transducer and activator of transcription 3 Homo sapiens 126-131 25434800-3 2015 Alantolactone effectively suppressed both constitutive and inducible STAT3 activation at tyrosine 705. Tyrosine 89-97 signal transducer and activator of transcription 3 Homo sapiens 69-74 25661370-5 2015 We proposed that PKM2 activates transcription of hypoxia inducible factor-1alpha (HIF-1alpha) by phosphorylating STAT3 (signal transducer and activator of transcription 3) at Y705 (tyrosine 705) as a plausible mechanism for liver cancer cell proliferation. Tyrosine 181-189 signal transducer and activator of transcription 3 Homo sapiens 113-118 25661370-5 2015 We proposed that PKM2 activates transcription of hypoxia inducible factor-1alpha (HIF-1alpha) by phosphorylating STAT3 (signal transducer and activator of transcription 3) at Y705 (tyrosine 705) as a plausible mechanism for liver cancer cell proliferation. Tyrosine 181-189 signal transducer and activator of transcription 3 Homo sapiens 120-170 25584415-2 2015 Activation of hypothalamic STAT-3 has been attributed to cytokine-promoted phosphorylation at tyrosine-705 (Tyr-705). Tyrosine 94-102 signal transducer and activator of transcription 3 Homo sapiens 27-33 25584415-2 2015 Activation of hypothalamic STAT-3 has been attributed to cytokine-promoted phosphorylation at tyrosine-705 (Tyr-705). Tyrosine 108-111 signal transducer and activator of transcription 3 Homo sapiens 27-33 25584415-5 2015 EGF and BK solely increased Ser-727 and IFN-gamma increased Tyr-705 phosphorylation of STAT-3. Tyrosine 60-63 signal transducer and activator of transcription 3 Homo sapiens 87-93 24671465-5 2015 The BM stroma-derived soluble factors could enhance the resistance of K562 cells to IM by increasing Stat3 phosphorylation on tyrosine 705 and subsequently increasing the expression of anti-apoptotic proteins and P-glycoprotein (P-gp) in K562 cells. Tyrosine 126-134 signal transducer and activator of transcription 3 Homo sapiens 101-106 25512602-8 2015 IL-10 induced phosphorylation of both Tyr(705) and Ser(727) residues of STAT3 in an AMPKalpha1-dependent manner, and these phosphorylation events were blocked by inhibition of Ca(2+)/calmodulin-dependent protein kinase kinase beta, an upstream activator of AMPK, and by the mTORC1 inhibitor rapamycin, respectively. Tyrosine 38-41 signal transducer and activator of transcription 3 Homo sapiens 72-77 25442996-12 2015 The results suggest that Stat3 activation (tyrosine phosphorylation) may be protective and that TLR4 inhibition could be targeted pharmacologically to treat calcific aortic valve disease. Tyrosine 43-51 signal transducer and activator of transcription 3 Homo sapiens 25-30 25393367-7 2015 SAHA downregulated the miR-17-92 cluster by abolishing tyrosine phosphorylation of STAT3 and decreased MCM7 transcription through localised histone deacetylation. Tyrosine 55-63 signal transducer and activator of transcription 3 Homo sapiens 83-88 25452693-5 2014 We further provide computational models that show that WFA binds STAT3 near the Y705 phospho-tyrosine residue of the STAT3 Src homology 2 (SH2) domain, suggesting that WFA prevents STAT3 dimer formation similar to BP-1-102, a well-established STAT3 inhibitor. Tyrosine 93-101 signal transducer and activator of transcription 3 Homo sapiens 65-70 25192910-7 2014 The results further show that tyrosine-phosphorylated STAT3 binds and activates the ALDH1 promoters in glioma cells. Tyrosine 30-38 signal transducer and activator of transcription 3 Homo sapiens 54-59 25622587-10 2014 CONCLUSIONS: bFGF mainly interacts with STAT3 tyrosine site-pSTAT3(Tyr705) to influence the level of STAT3 phosphorylation;blocking bFGF/STAT3 signaling pathway can induce glioma cell apoptosis through mitochondrial pathway. Tyrosine 46-54 signal transducer and activator of transcription 3 Homo sapiens 40-45 25622587-10 2014 CONCLUSIONS: bFGF mainly interacts with STAT3 tyrosine site-pSTAT3(Tyr705) to influence the level of STAT3 phosphorylation;blocking bFGF/STAT3 signaling pathway can induce glioma cell apoptosis through mitochondrial pathway. Tyrosine 46-54 signal transducer and activator of transcription 3 Homo sapiens 61-66 25622587-10 2014 CONCLUSIONS: bFGF mainly interacts with STAT3 tyrosine site-pSTAT3(Tyr705) to influence the level of STAT3 phosphorylation;blocking bFGF/STAT3 signaling pathway can induce glioma cell apoptosis through mitochondrial pathway. Tyrosine 46-54 signal transducer and activator of transcription 3 Homo sapiens 61-66 25383546-5 2014 The mechanism of TBr mediated inhibition of p-STAT3 was found to be due to the activation of ubiquitin dependent degradation of tyrosine 705 and serine 727 p-STAT3. Tyrosine 128-136 signal transducer and activator of transcription 3 Homo sapiens 46-51 26279438-9 2015 Phosphorylation of STAT3 was identified mainly at the tyrosine in position 705 (Y705), and the Y705F mutants attenuated IL-17-mediated STAT3 activation. Tyrosine 54-62 signal transducer and activator of transcription 3 Homo sapiens 19-24 25687700-6 2015 This interferon- gamma-induced astrocytic neurotoxicity is mediated by the activation of the Janus kinase-signal transducer and activator of transcription (STAT) 3 pathway in the astrocytes, and involves intracellular phosphorylation of STAT3 at tyrosine-705 residue. Tyrosine 246-254 signal transducer and activator of transcription 3 Homo sapiens 156-160 25452693-5 2014 We further provide computational models that show that WFA binds STAT3 near the Y705 phospho-tyrosine residue of the STAT3 Src homology 2 (SH2) domain, suggesting that WFA prevents STAT3 dimer formation similar to BP-1-102, a well-established STAT3 inhibitor. Tyrosine 93-101 signal transducer and activator of transcription 3 Homo sapiens 117-122 25452693-5 2014 We further provide computational models that show that WFA binds STAT3 near the Y705 phospho-tyrosine residue of the STAT3 Src homology 2 (SH2) domain, suggesting that WFA prevents STAT3 dimer formation similar to BP-1-102, a well-established STAT3 inhibitor. Tyrosine 93-101 signal transducer and activator of transcription 3 Homo sapiens 117-122 25452693-5 2014 We further provide computational models that show that WFA binds STAT3 near the Y705 phospho-tyrosine residue of the STAT3 Src homology 2 (SH2) domain, suggesting that WFA prevents STAT3 dimer formation similar to BP-1-102, a well-established STAT3 inhibitor. Tyrosine 93-101 signal transducer and activator of transcription 3 Homo sapiens 117-122 25016575-6 2014 In this study, we show that in activated human memory B cells, NAC inhibited STAT3 phosphorylation on tyrosine 705 but not on Serine 727. Tyrosine 102-110 signal transducer and activator of transcription 3 Homo sapiens 77-82 25072659-3 2014 We observed that the diabetic environment 1) enhanced tyrosine phosphorylation of JAK2 and STAT3; 2) induced nuclear localization of tyrosine phosphorylated STAT3 through a reactive oxygen species-mediated mechanism, with angiotensin II stimulation further enhancing STAT3 nuclear accumulation; and 3) stimulated collagen I production. Tyrosine 54-62 signal transducer and activator of transcription 3 Homo sapiens 91-96 25072659-3 2014 We observed that the diabetic environment 1) enhanced tyrosine phosphorylation of JAK2 and STAT3; 2) induced nuclear localization of tyrosine phosphorylated STAT3 through a reactive oxygen species-mediated mechanism, with angiotensin II stimulation further enhancing STAT3 nuclear accumulation; and 3) stimulated collagen I production. Tyrosine 54-62 signal transducer and activator of transcription 3 Homo sapiens 157-162 25072659-3 2014 We observed that the diabetic environment 1) enhanced tyrosine phosphorylation of JAK2 and STAT3; 2) induced nuclear localization of tyrosine phosphorylated STAT3 through a reactive oxygen species-mediated mechanism, with angiotensin II stimulation further enhancing STAT3 nuclear accumulation; and 3) stimulated collagen I production. Tyrosine 54-62 signal transducer and activator of transcription 3 Homo sapiens 157-162 25072659-3 2014 We observed that the diabetic environment 1) enhanced tyrosine phosphorylation of JAK2 and STAT3; 2) induced nuclear localization of tyrosine phosphorylated STAT3 through a reactive oxygen species-mediated mechanism, with angiotensin II stimulation further enhancing STAT3 nuclear accumulation; and 3) stimulated collagen I production. Tyrosine 133-141 signal transducer and activator of transcription 3 Homo sapiens 91-96 25072659-3 2014 We observed that the diabetic environment 1) enhanced tyrosine phosphorylation of JAK2 and STAT3; 2) induced nuclear localization of tyrosine phosphorylated STAT3 through a reactive oxygen species-mediated mechanism, with angiotensin II stimulation further enhancing STAT3 nuclear accumulation; and 3) stimulated collagen I production. Tyrosine 133-141 signal transducer and activator of transcription 3 Homo sapiens 157-162 25072659-3 2014 We observed that the diabetic environment 1) enhanced tyrosine phosphorylation of JAK2 and STAT3; 2) induced nuclear localization of tyrosine phosphorylated STAT3 through a reactive oxygen species-mediated mechanism, with angiotensin II stimulation further enhancing STAT3 nuclear accumulation; and 3) stimulated collagen I production. Tyrosine 133-141 signal transducer and activator of transcription 3 Homo sapiens 157-162 25153147-4 2014 Using reporter assays and site-directed mutagenesis we show that the frog LepR signals via signal transducer and activator of transcription (STAT) 3 and STAT5 through evolutionarily conserved tyrosine residues in the LepR cytoplasmic domain. Tyrosine 192-200 signal transducer and activator of transcription 3 Homo sapiens 91-148 25398805-5 2014 RESULTS: After treatment with H2O2, DHT decreased tyrosine phosphorylation/transcriptional activity of STAT3 and promoter activity of MnSOD while E2 increased them. Tyrosine 50-58 signal transducer and activator of transcription 3 Homo sapiens 103-108 25217633-1 2014 STAT3 is a pleiotropic transcription factor that is activated by the phosphorylation of tyrosine 705 in response to many cytokines and growth factors. Tyrosine 88-96 signal transducer and activator of transcription 3 Homo sapiens 0-5 25217633-2 2014 STAT3 without Tyr-705 phosphorylation (U-STAT3) is also a potent transcription factor, and its concentration in cells increases greatly in response to STAT3 activation because the STAT3 gene can be driven by phosphorylated STAT3 dimers. Tyrosine 14-17 signal transducer and activator of transcription 3 Homo sapiens 41-46 25217633-2 2014 STAT3 without Tyr-705 phosphorylation (U-STAT3) is also a potent transcription factor, and its concentration in cells increases greatly in response to STAT3 activation because the STAT3 gene can be driven by phosphorylated STAT3 dimers. Tyrosine 14-17 signal transducer and activator of transcription 3 Homo sapiens 0-5 25217633-2 2014 STAT3 without Tyr-705 phosphorylation (U-STAT3) is also a potent transcription factor, and its concentration in cells increases greatly in response to STAT3 activation because the STAT3 gene can be driven by phosphorylated STAT3 dimers. Tyrosine 14-17 signal transducer and activator of transcription 3 Homo sapiens 41-46 25217633-2 2014 STAT3 without Tyr-705 phosphorylation (U-STAT3) is also a potent transcription factor, and its concentration in cells increases greatly in response to STAT3 activation because the STAT3 gene can be driven by phosphorylated STAT3 dimers. Tyrosine 14-17 signal transducer and activator of transcription 3 Homo sapiens 41-46 25217633-2 2014 STAT3 without Tyr-705 phosphorylation (U-STAT3) is also a potent transcription factor, and its concentration in cells increases greatly in response to STAT3 activation because the STAT3 gene can be driven by phosphorylated STAT3 dimers. Tyrosine 14-17 signal transducer and activator of transcription 3 Homo sapiens 41-46 25217633-5 2014 In contrast, we found only a very minor role for Lys-685 in gene expression induced in response to tyrosine-phosphorylated STAT3. Tyrosine 99-107 signal transducer and activator of transcription 3 Homo sapiens 123-128 25299776-5 2014 We observed that leukemic but not normal B cells from CLL patients exhibit constitutive activation of an atypical form of the STAT3 signaling factor, phosphorylated on serine 727 (Ser(727)) in the absence of detectable canonical tyrosine 705 (Tyr705)-dependent activation in vivo. Tyrosine 229-237 signal transducer and activator of transcription 3 Homo sapiens 126-131 24890449-10 2014 Pharmacological inhibition of JAK2 and Src blunted tyrosine phosphorylation of EGFR and STAT3, while treatment with an EGFR tyrosine kinase inhibitor gefitinib inhibited phosphorylation of STAT3 without affecting that of JAK2 and Src in HCT116 cells. Tyrosine 51-59 signal transducer and activator of transcription 3 Homo sapiens 88-93 25101566-3 2014 RESULTS: Lupeol suppressed constitutive activation of STAT3 phosphorylation at tyrosine 705 residue effectively in a dose- and time-dependent manner. Tyrosine 79-87 signal transducer and activator of transcription 3 Homo sapiens 54-59 24721162-4 2014 Here, we show that an arginine-glutamine-exchange at the STAT3 moieties R414 and R417 (R414Q and R417Q) reduces cytokine-dependent tyrosine phosphorylation of STAT3. Tyrosine 131-139 signal transducer and activator of transcription 3 Homo sapiens 57-62 24721162-4 2014 Here, we show that an arginine-glutamine-exchange at the STAT3 moieties R414 and R417 (R414Q and R417Q) reduces cytokine-dependent tyrosine phosphorylation of STAT3. Tyrosine 131-139 signal transducer and activator of transcription 3 Homo sapiens 159-164 24721162-13 2014 Compared to wild-type STAT3, the STAT3 R414K/R417K mutant shows attenuated tyrosine phosphorylation and it is a less active transcription factor. Tyrosine 75-83 signal transducer and activator of transcription 3 Homo sapiens 22-27 24721162-13 2014 Compared to wild-type STAT3, the STAT3 R414K/R417K mutant shows attenuated tyrosine phosphorylation and it is a less active transcription factor. Tyrosine 75-83 signal transducer and activator of transcription 3 Homo sapiens 33-38 24726840-0 2014 Reduced phosphorylation of Stat3 at Ser-727 mediated by casein kinase 2 - protein phosphatase 2A enhances Stat3 Tyr-705 induced tumorigenic potential of glioma cells. Tyrosine 112-115 signal transducer and activator of transcription 3 Homo sapiens 27-32 24726840-0 2014 Reduced phosphorylation of Stat3 at Ser-727 mediated by casein kinase 2 - protein phosphatase 2A enhances Stat3 Tyr-705 induced tumorigenic potential of glioma cells. Tyrosine 112-115 signal transducer and activator of transcription 3 Homo sapiens 106-111 24726840-1 2014 Signal transducer and activator of transcription 3 (Stat3) is a transcription factor that is involved in cell survival and proliferation and has been found to be persistently activated in most human cancers mainly through its phosphorylation at Tyr-705. Tyrosine 245-248 signal transducer and activator of transcription 3 Homo sapiens 0-50 24726840-1 2014 Signal transducer and activator of transcription 3 (Stat3) is a transcription factor that is involved in cell survival and proliferation and has been found to be persistently activated in most human cancers mainly through its phosphorylation at Tyr-705. Tyrosine 245-248 signal transducer and activator of transcription 3 Homo sapiens 52-57 24726840-7 2014 Stat3 Tyr-705 residue was conversely phosphorylated in similar situations. Tyrosine 6-9 signal transducer and activator of transcription 3 Homo sapiens 0-5 24978112-2 2014 Phosphorylation of a tyrosine at amino acid Y705 is essential for the function of STAT3. Tyrosine 21-29 signal transducer and activator of transcription 3 Homo sapiens 82-87 25089666-1 2014 STAT3 mediates cytokine and growth factor receptor signalling, becoming transcriptionally active upon tyrosine 705 phosphorylation (Y-P). Tyrosine 102-110 signal transducer and activator of transcription 3 Homo sapiens 0-5 24671708-4 2014 CMV-induced diaphragm atrophy and contractile dysfunction coincided with marked increases in STAT3 phosphorylation on both tyrosine 705 (Tyr705) and serine 727 (Ser727). Tyrosine 123-131 signal transducer and activator of transcription 3 Homo sapiens 93-98 24903225-7 2014 SC-2001 inhibited cell growth and induced apoptosis in association with downregulation of p-STAT3 (Tyr 705) in breast cancer cells. Tyrosine 99-102 signal transducer and activator of transcription 3 Homo sapiens 92-97 24891320-0 2014 Transcription of the activating receptor NKG2D in natural killer cells is regulated by STAT3 tyrosine phosphorylation. Tyrosine 93-101 signal transducer and activator of transcription 3 Homo sapiens 87-92 24847057-3 2014 The up-regulation of HAS2 was associated with JAK2 and ERK1/2 activation, and specific Tyr(705) phosphorylation of transcription factor STAT3. Tyrosine 87-90 signal transducer and activator of transcription 3 Homo sapiens 136-141 24847057-5 2014 Chromatin immunoprecipitations demonstrated increased promoter binding of Tyr(P)(705)-STAT3 at the time of HAS2 induction. Tyrosine 74-77 signal transducer and activator of transcription 3 Homo sapiens 86-91 24164699-5 2014 Expression of tyrosine-705 phosphorylated STAT3 (pSTAT3) was determined immunohistochemically in 79 PDACs. Tyrosine 14-22 signal transducer and activator of transcription 3 Homo sapiens 42-47 24063605-5 2014 NO produced by iNOS or treatment of microglia with exogenous GSNO inhibited STAT3 activation via inhibiting STAT3 phosphorylation (Tyr(705)). Tyrosine 131-134 signal transducer and activator of transcription 3 Homo sapiens 76-81 24063605-5 2014 NO produced by iNOS or treatment of microglia with exogenous GSNO inhibited STAT3 activation via inhibiting STAT3 phosphorylation (Tyr(705)). Tyrosine 131-134 signal transducer and activator of transcription 3 Homo sapiens 108-113 24063605-10 2014 INNOVATION: Microglial proliferation is regulated by NO via S-nitrosylation of STAT3 (Cys(259)) and inhibition of STAT3 (Tyr(705)) phosphorylation. Tyrosine 121-124 signal transducer and activator of transcription 3 Homo sapiens 114-119 24686183-7 2014 Immunoreactivity against JAK1 and STAT3 was observed mainly in chondrocytes, whereas immunoreactivity against p-STAT3 at Tyr(705) was observed in the nuclei of chondrocytes. Tyrosine 121-124 signal transducer and activator of transcription 3 Homo sapiens 112-117 24686183-5 2014 The expression of JAK1, STAT3, and p-STAT3 at Tyr(705) was also examined by immunostaining. Tyrosine 46-49 signal transducer and activator of transcription 3 Homo sapiens 37-42 24930769-5 2014 In human cancer cells, these dual inhibitors block the auto-phosphorylation of Aurora A (Thr-288) and the phosphorylation of the Aurora B substrate histone H3 (Ser-10) and the JAK2 substrate STAT3 (Tyr-705). Tyrosine 198-201 signal transducer and activator of transcription 3 Homo sapiens 191-196 24561183-7 2014 Leptin stimulates acute activation of the transcription factor STAT3 via phosphorylation of tyrosine 705. Tyrosine 92-100 signal transducer and activator of transcription 3 Homo sapiens 63-68 24627483-5 2014 Suppression of STAT3 tyrosine phosphorylation coordinately decreased the mRNA and protein levels of the beta subunits of the 20 S core complex in DU145 cells. Tyrosine 21-29 signal transducer and activator of transcription 3 Homo sapiens 15-20 24615012-2 2014 STAT3 activity is regulated by numerous posttranslational modifications (PTMs), including Tyr(705) phosphorylation, which is widely used as an indicator of canonical STAT3 function. Tyrosine 90-93 signal transducer and activator of transcription 3 Homo sapiens 0-5 24615012-2 2014 STAT3 activity is regulated by numerous posttranslational modifications (PTMs), including Tyr(705) phosphorylation, which is widely used as an indicator of canonical STAT3 function. Tyrosine 90-93 signal transducer and activator of transcription 3 Homo sapiens 166-171 24615012-3 2014 Here, we report a noncanonical mechanism of STAT3 activation that occurs independently of Tyr(705) phosphorylation. Tyrosine 90-93 signal transducer and activator of transcription 3 Homo sapiens 44-49 24846175-6 2014 Increased dimerization subsequently reduces PTPRT"s catalytic activity on the dephosphorylation of signal transducer and activator of transcription 3 (STAT3) at tyrosine residue 705 (pY705 STAT3), then the accumulated pY705 STAT3 translocates into the nucleus. Tyrosine 161-169 signal transducer and activator of transcription 3 Homo sapiens 99-149 24846175-6 2014 Increased dimerization subsequently reduces PTPRT"s catalytic activity on the dephosphorylation of signal transducer and activator of transcription 3 (STAT3) at tyrosine residue 705 (pY705 STAT3), then the accumulated pY705 STAT3 translocates into the nucleus. Tyrosine 161-169 signal transducer and activator of transcription 3 Homo sapiens 151-156 24846175-6 2014 Increased dimerization subsequently reduces PTPRT"s catalytic activity on the dephosphorylation of signal transducer and activator of transcription 3 (STAT3) at tyrosine residue 705 (pY705 STAT3), then the accumulated pY705 STAT3 translocates into the nucleus. Tyrosine 161-169 signal transducer and activator of transcription 3 Homo sapiens 189-194 24846175-6 2014 Increased dimerization subsequently reduces PTPRT"s catalytic activity on the dephosphorylation of signal transducer and activator of transcription 3 (STAT3) at tyrosine residue 705 (pY705 STAT3), then the accumulated pY705 STAT3 translocates into the nucleus. Tyrosine 161-169 signal transducer and activator of transcription 3 Homo sapiens 189-194 24569879-2 2014 In response to cytokine stimulation, STAT3 is activated through phosphorylation of a single tyrosine residue. Tyrosine 92-100 signal transducer and activator of transcription 3 Homo sapiens 37-42 24446131-6 2014 In contrast, the inhibition of STAT3 activity in HoxB4-overexpressing hematopoietic cells significantly abrogated the enhancing effects of HoxB4, and the upregulation of HoxB4 caused a ligand-independent Tyr-phosphorylation of STAT3. Tyrosine 204-207 signal transducer and activator of transcription 3 Homo sapiens 31-36 24733900-0 2014 Selective inhibition of the function of tyrosine-phosphorylated STAT3 with a phosphorylation site-specific intrabody. Tyrosine 40-48 signal transducer and activator of transcription 3 Homo sapiens 64-69 24658061-4 2014 RKIP over expression inhibited c-Src auto-phosphorylation and activation, as well as IL-6-, JAK1 and 2-, and activated Raf-mediated STAT3 tyrosine and serine phosphorylation and subsequent activation. Tyrosine 138-146 signal transducer and activator of transcription 3 Homo sapiens 132-137 24736071-7 2014 SC-1 reduced STAT3 phosphorylation at tyrosine 705 in all tested EGFR wild-type NSCLC cells. Tyrosine 38-46 signal transducer and activator of transcription 3 Homo sapiens 13-18 24333736-3 2014 We found that CPS specifically inhibited both constitutive and inducible STAT3 activation at tyrosine residue 705 but not at serine residue 727 in human multiple myeloma cells. Tyrosine 93-101 signal transducer and activator of transcription 3 Homo sapiens 73-78 24670366-3 2014 High Src activity was found to correlate with high levels of the Src effector, Signal Transducer and Activator of Transcription-3 (Stat3) in its tyrosine-705 phosphorylated, i.e., transcriptionally activated form, in the majority of Non-Small Cell Lung Cancer lines examined. Tyrosine 145-153 signal transducer and activator of transcription 3 Homo sapiens 79-129 24670366-3 2014 High Src activity was found to correlate with high levels of the Src effector, Signal Transducer and Activator of Transcription-3 (Stat3) in its tyrosine-705 phosphorylated, i.e., transcriptionally activated form, in the majority of Non-Small Cell Lung Cancer lines examined. Tyrosine 145-153 signal transducer and activator of transcription 3 Homo sapiens 131-136 24658061-10 2014 Moreover, MTI treatment resulted in a decrease in Src-mediated STAT3 tyrosine phosphorylation and activation, an effect that was significantly enhanced by RKIP over expression. Tyrosine 69-77 signal transducer and activator of transcription 3 Homo sapiens 63-68 24095958-5 2013 Furthermore, glutathione ethylene ester, the cell permeable form of GSH, reverts the inhibitory action of cynaropicrin on STAT3 tyrosine phosphorylation. Tyrosine 128-136 signal transducer and activator of transcription 3 Homo sapiens 122-127 23913134-5 2014 Importantly, HDAC3 inhibition downregulates phosphorylation (tyrosine 705 and serine 727) of signal transducers and activators of transcription 3 (STAT3). Tyrosine 61-69 signal transducer and activator of transcription 3 Homo sapiens 93-145 23913134-5 2014 Importantly, HDAC3 inhibition downregulates phosphorylation (tyrosine 705 and serine 727) of signal transducers and activators of transcription 3 (STAT3). Tyrosine 61-69 signal transducer and activator of transcription 3 Homo sapiens 147-152 23689514-2 2014 Here, we demonstrate that STAT3 activation mostly results from constitutive Janus kinase (JAK)3 phosphorylation on tyrosine 980, as observed in three of the four tested NKCL cell lines and in 20 of the 23 NKCL tumor samples under study. Tyrosine 115-123 signal transducer and activator of transcription 3 Homo sapiens 26-31 24410832-4 2014 RESULTS: We found that FTO protein expression is regulated by both leptin and IL-6, concomitantly to an induction of STAT3 tyrosine phosphorylation, in leptin receptor (LepRb) expressing HuH7 cells. Tyrosine 123-131 signal transducer and activator of transcription 3 Homo sapiens 117-122 24883303-3 2014 The tissue levels of VEGF, Th-2 cytokines IL-6 and IL-8, IL-8 receptor CXCR2, and tyrosine p-STAT-3/SOCS-3 axis modulating cytokine expression were evaluated immunohistochemically in 62 CLL/SLL cases. Tyrosine 82-90 signal transducer and activator of transcription 3 Homo sapiens 93-99 24154873-6 2014 Here, we demonstrate that DeltaNp63 overexpression, independent of p53, increases secretion of interleukin (IL)-6 and IL-8, leading to elevated phosphorylation of STAT3 (Tyr-705). Tyrosine 170-173 signal transducer and activator of transcription 3 Homo sapiens 163-168 24319264-10 2014 We conclude that the inhibition of canonical STAT1 and STAT3 target gene expression abrogates their intrinsic negative feedback loops, leading to accumulation of phospho-tyrosine-STAT3 and prolonged STAT1 phosphorylation. Tyrosine 170-178 signal transducer and activator of transcription 3 Homo sapiens 55-60 24319264-10 2014 We conclude that the inhibition of canonical STAT1 and STAT3 target gene expression abrogates their intrinsic negative feedback loops, leading to accumulation of phospho-tyrosine-STAT3 and prolonged STAT1 phosphorylation. Tyrosine 170-178 signal transducer and activator of transcription 3 Homo sapiens 179-184 24028796-3 2013 The levels of signal transduction and activation of transcription 3 (STAT3) tyrosine phosphorylation and caspase3 expression were determined using Western blot analysis, and the apoptosis of decidual gammadelta T cells was analyzed by flow cytometry. Tyrosine 76-84 signal transducer and activator of transcription 3 Homo sapiens 69-74 24423131-6 2013 Phosphorylation at tyrosine 705 of STAT3 was decreased by treatment with everolimus in a dose-dependent manner in HaCaT cells; in contrast, phosphorylation at serine 727 was not decreased by everolimus, but slightly increased. Tyrosine 19-27 signal transducer and activator of transcription 3 Homo sapiens 35-40 24192293-2 2013 In response to cytokine stimulation STAT3 is phosphorylated on a single tyrosine residue at position 705, dimerizes and accumulates in the nucleus to induce target gene expression. Tyrosine 72-80 signal transducer and activator of transcription 3 Homo sapiens 36-41 24192293-5 2013 RESULTS: Expression of STAT3-YF-YFP impairs tyrosine phosphorylation, nuclear translocation and the transcriptional activity of WT-STAT3 in IL-6-stimulated cells. Tyrosine 44-52 signal transducer and activator of transcription 3 Homo sapiens 23-28 23673666-3 2013 Using site-directed mutagenesis and deletion variants of the murine and human IL-23R, we showed that the predicted STAT binding sites (pYXXQ; including Tyr-504 and Tyr-626 in murine IL-23R and Tyr-484 and Tyr-611 in human IL-23R) mediated STAT3 activation. Tyrosine 152-155 signal transducer and activator of transcription 3 Homo sapiens 239-244 24093956-8 2013 Western blotting analysis indicated that LBH589 could decrease the expression of gankyrin and subsequently reduced serine-phosphorylated Akt and tyrosine-phosphorylated STAT3 expression although the total Akt and STAT3 were unaffected. Tyrosine 145-153 signal transducer and activator of transcription 3 Homo sapiens 169-174 23962443-4 2013 Sophoraflavanone G inhibited tyrosine phosphorylation of STAT proteins in Hodgkin"s lymphoma and tyrosine phosphorylation of STAT3 in solid cancer cells by inhibiting phosphorylation of the Janus kinase (JAK) proteins, Src family tyrosine kinases, such as Lyn and Src, Akt, and ERK1/2. Tyrosine 97-105 signal transducer and activator of transcription 3 Homo sapiens 125-130 24101906-5 2013 Most mature cells studied thus far rely on tyrosine phosphorylation (STAT3-Tyr) to promote survival and growth; in contrast, STAT3-Tyr induces the differentiation of neural stem cells (NSCs). Tyrosine 43-51 signal transducer and activator of transcription 3 Homo sapiens 69-74 24470978-11 2013 Distinctly, STAT3, STAT5, and STAT6 are continually imported to the nucleus independent of tyrosine phosphorylation. Tyrosine 91-99 signal transducer and activator of transcription 3 Homo sapiens 12-17 24082147-1 2013 The activation of STAT3 by tyrosine phosphorylation, essential for normal development and for a normal inflammatory response to invading pathogens, is kept in check by negative regulators. Tyrosine 27-35 signal transducer and activator of transcription 3 Homo sapiens 18-23 24082147-3 2013 SOCS3, the major negative regulator of STAT3, is induced by tyrosine-phosphorylated STAT3 and terminates STAT3 phosphorylation about 2 h after initial exposure of cells to members of the IL-6 family of cytokines by binding cooperatively to the common receptor subunit gp130 and JAKs 1 and 2. Tyrosine 60-68 signal transducer and activator of transcription 3 Homo sapiens 39-44 24082147-3 2013 SOCS3, the major negative regulator of STAT3, is induced by tyrosine-phosphorylated STAT3 and terminates STAT3 phosphorylation about 2 h after initial exposure of cells to members of the IL-6 family of cytokines by binding cooperatively to the common receptor subunit gp130 and JAKs 1 and 2. Tyrosine 60-68 signal transducer and activator of transcription 3 Homo sapiens 84-89 24082147-3 2013 SOCS3, the major negative regulator of STAT3, is induced by tyrosine-phosphorylated STAT3 and terminates STAT3 phosphorylation about 2 h after initial exposure of cells to members of the IL-6 family of cytokines by binding cooperatively to the common receptor subunit gp130 and JAKs 1 and 2. Tyrosine 60-68 signal transducer and activator of transcription 3 Homo sapiens 84-89 23903834-1 2013 In the canonical STAT3 signaling pathway, binding of agonist to receptors activates Janus kinases that phosphorylate cytoplasmic STAT3 at tyrosine 705 (Y705). Tyrosine 138-146 signal transducer and activator of transcription 3 Homo sapiens 17-22 23903834-1 2013 In the canonical STAT3 signaling pathway, binding of agonist to receptors activates Janus kinases that phosphorylate cytoplasmic STAT3 at tyrosine 705 (Y705). Tyrosine 138-146 signal transducer and activator of transcription 3 Homo sapiens 129-134 23874455-4 2013 Pharmacologic targeting of STAT3 expression in cervical cancer cell lines either by STAT3-specific siRNA or blocking its tyrosine phosphorylation by AG490 or curcumin led to dose-dependent accumulation of p53 and pRb in cervical cancer cells. Tyrosine 121-129 signal transducer and activator of transcription 3 Homo sapiens 27-32 23673666-3 2013 Using site-directed mutagenesis and deletion variants of the murine and human IL-23R, we showed that the predicted STAT binding sites (pYXXQ; including Tyr-504 and Tyr-626 in murine IL-23R and Tyr-484 and Tyr-611 in human IL-23R) mediated STAT3 activation. Tyrosine 164-167 signal transducer and activator of transcription 3 Homo sapiens 239-244 23673666-3 2013 Using site-directed mutagenesis and deletion variants of the murine and human IL-23R, we showed that the predicted STAT binding sites (pYXXQ; including Tyr-504 and Tyr-626 in murine IL-23R and Tyr-484 and Tyr-611 in human IL-23R) mediated STAT3 activation. Tyrosine 164-167 signal transducer and activator of transcription 3 Homo sapiens 239-244 23673666-3 2013 Using site-directed mutagenesis and deletion variants of the murine and human IL-23R, we showed that the predicted STAT binding sites (pYXXQ; including Tyr-504 and Tyr-626 in murine IL-23R and Tyr-484 and Tyr-611 in human IL-23R) mediated STAT3 activation. Tyrosine 164-167 signal transducer and activator of transcription 3 Homo sapiens 239-244 23673666-7 2013 A third predicted site, Tyr-416 in murine and Tyr-397 in human IL-23R, is involved in the activation of PI3K/Akt and the MAPK pathway leading to STAT3-independent proliferation of Ba/F3 cells upon stimulation with IL-23. Tyrosine 24-27 signal transducer and activator of transcription 3 Homo sapiens 145-150 23673666-7 2013 A third predicted site, Tyr-416 in murine and Tyr-397 in human IL-23R, is involved in the activation of PI3K/Akt and the MAPK pathway leading to STAT3-independent proliferation of Ba/F3 cells upon stimulation with IL-23. Tyrosine 46-49 signal transducer and activator of transcription 3 Homo sapiens 145-150 23686482-8 2013 Both Tyr(705) phosphorylation and binding of STAT3 on MICA promoter are reduced by GSK3 inhibitors; in addition, overexpression of a constitutively active form of STAT3 significantly inhibits MICA upregulation. Tyrosine 5-8 signal transducer and activator of transcription 3 Homo sapiens 163-168 24069557-4 2013 In the cytoplasm, in the absence of conventional phosphorylation on the tyrosine 705 residue, STAT3 interacts with the PKR kinase to inhibit eIF2A phosphorylation and so reduce autophagic pathways. Tyrosine 72-80 signal transducer and activator of transcription 3 Homo sapiens 94-99 24069560-4 2013 The canonical activation model of STAT3 relies on a constitutive phosphorylation on its 705 tyrosine site, resulting in its dimerization, nuclear translocation, and the consequent activation of cancer genes. Tyrosine 92-100 signal transducer and activator of transcription 3 Homo sapiens 34-39 24069560-6 2013 However, recent results have shown that STAT3 can function as an oncogene in the absence of this tyrosine phosphorylation. Tyrosine 97-105 signal transducer and activator of transcription 3 Homo sapiens 40-45 23303450-3 2013 HSF showed increased Tyr 705 STAT3 phosphorylation compared with normal fibroblast (NF) after IL-6 IL-6Ralpha stimulation by immunoassays. Tyrosine 21-24 signal transducer and activator of transcription 3 Homo sapiens 29-34 23684459-4 2013 Here, we show that EZH2 binds to and methylates STAT3, leading to enhanced STAT3 activity by increased tyrosine phosphorylation of STAT3. Tyrosine 103-111 signal transducer and activator of transcription 3 Homo sapiens 48-53 23684459-4 2013 Here, we show that EZH2 binds to and methylates STAT3, leading to enhanced STAT3 activity by increased tyrosine phosphorylation of STAT3. Tyrosine 103-111 signal transducer and activator of transcription 3 Homo sapiens 75-80 23684459-4 2013 Here, we show that EZH2 binds to and methylates STAT3, leading to enhanced STAT3 activity by increased tyrosine phosphorylation of STAT3. Tyrosine 103-111 signal transducer and activator of transcription 3 Homo sapiens 75-80 23340480-8 2013 Studying the signalling pathway proteins in the uNK cells, an activation of STAT3/Tyr by TPO, was detected. Tyrosine 82-85 signal transducer and activator of transcription 3 Homo sapiens 76-81 23279849-0 2013 Morin inhibits STAT3 tyrosine 705 phosphorylation in tumor cells through activation of protein tyrosine phosphatase SHP1. Tyrosine 21-29 signal transducer and activator of transcription 3 Homo sapiens 15-20 23322008-1 2013 STAT3-STAT3 dimerization, which involves reciprocal binding of the STAT3-SH2 domain to phosphorylated tyrosine-705 (Y-705), is required for STAT3 nuclear translocation, DNA binding, and transcriptional regulation of downstream target genes. Tyrosine 102-110 signal transducer and activator of transcription 3 Homo sapiens 0-5 23446809-0 2013 Tumor STAT3 tyrosine phosphorylation status, as a predictor of benefit from adjuvant chemotherapy for breast cancer. Tyrosine 12-20 signal transducer and activator of transcription 3 Homo sapiens 6-11 23322008-1 2013 STAT3-STAT3 dimerization, which involves reciprocal binding of the STAT3-SH2 domain to phosphorylated tyrosine-705 (Y-705), is required for STAT3 nuclear translocation, DNA binding, and transcriptional regulation of downstream target genes. Tyrosine 102-110 signal transducer and activator of transcription 3 Homo sapiens 6-11 23322008-1 2013 STAT3-STAT3 dimerization, which involves reciprocal binding of the STAT3-SH2 domain to phosphorylated tyrosine-705 (Y-705), is required for STAT3 nuclear translocation, DNA binding, and transcriptional regulation of downstream target genes. Tyrosine 102-110 signal transducer and activator of transcription 3 Homo sapiens 6-11 23322008-1 2013 STAT3-STAT3 dimerization, which involves reciprocal binding of the STAT3-SH2 domain to phosphorylated tyrosine-705 (Y-705), is required for STAT3 nuclear translocation, DNA binding, and transcriptional regulation of downstream target genes. Tyrosine 102-110 signal transducer and activator of transcription 3 Homo sapiens 6-11 23174178-10 2013 The localizations of STAT1 and STAT3 were revealed to be present in fibroblasts in human CSDH outer membranes, especially when p-STAT3 at Tyr(705) was in the nuclei of fibroblasts. Tyrosine 138-141 signal transducer and activator of transcription 3 Homo sapiens 31-36 23426146-9 2013 The transient expression of GSTP1 specifically downregulated epidermal growth factor (EGF)-mediated tyrosine phosphorylation of Stat3, and subsequently suppressed the transcriptional activity of Stat3. Tyrosine 100-108 signal transducer and activator of transcription 3 Homo sapiens 128-133 22450744-5 2013 In this study, we demonstrate that p14(ARF) promotes apoptosis in lung tumour cells harbouring the EGFR L858R mutation through the accumulation of phosphorylated signal transducer and activator of transcription 3 (STAT3) on Tyr 705 residue, which leads to Bcl-2 downregulation. Tyrosine 224-227 signal transducer and activator of transcription 3 Homo sapiens 162-212 22450744-6 2013 Using siRNA against PTP-RT, the phosphatase that specifically targets Tyr 705 residue, we show that accumulation of pSTAT3-Tyr705 promotes EGFR L858R mutant cell death, thereby confirming the existence of a STAT3-dependent pro-apoptotic pathway in these cells. Tyrosine 70-73 signal transducer and activator of transcription 3 Homo sapiens 117-122 23174178-8 2013 The expression of p-STAT3 at Tyr(705) is more significant compared with that of p-STAT1 at Tyr(701). Tyrosine 29-32 signal transducer and activator of transcription 3 Homo sapiens 20-25 23174178-10 2013 The localizations of STAT1 and STAT3 were revealed to be present in fibroblasts in human CSDH outer membranes, especially when p-STAT3 at Tyr(705) was in the nuclei of fibroblasts. Tyrosine 138-141 signal transducer and activator of transcription 3 Homo sapiens 129-134 23166300-1 2013 Tyrosine phosphorylation-dependent signaling, as mediated by members of the epidermal growth factor receptor (EGFR) family (ErbB1 to -4) of protein tyrosine kinases (PTKs), Src family PTKs (SFKs), and cytokines such as interleukin-6 (IL-6) that signal via signal transducer and activator of transcription 3 (STAT3), is critical to the development and progression of many human breast cancers. Tyrosine 0-8 signal transducer and activator of transcription 3 Homo sapiens 256-306 22978563-5 2013 Both sorafenib and SC-49 down-regulated the phosphorylation of STAT3 at Tyr(705) and subsequently reduced the levels of STAT3-regulated proteins, Mcl-1, survivin and cylcin D1, in CS-1008-treated HCC cells. Tyrosine 72-75 signal transducer and activator of transcription 3 Homo sapiens 63-68 23166300-1 2013 Tyrosine phosphorylation-dependent signaling, as mediated by members of the epidermal growth factor receptor (EGFR) family (ErbB1 to -4) of protein tyrosine kinases (PTKs), Src family PTKs (SFKs), and cytokines such as interleukin-6 (IL-6) that signal via signal transducer and activator of transcription 3 (STAT3), is critical to the development and progression of many human breast cancers. Tyrosine 0-8 signal transducer and activator of transcription 3 Homo sapiens 308-313 23086340-6 2013 STAT1 phosphorylation attenuated VEGF-A-induced STAT3 tyrosine phosphorylation in Tie2-R849W*-expressing HUVECs. Tyrosine 54-62 signal transducer and activator of transcription 3 Homo sapiens 48-53 23032719-4 2013 We found that evodiamine suppressed both constitutive and interleukin-6 (IL-6)-induced activation of STAT3 tyrosine 705 (Tyr(705)) effectively. Tyrosine 107-115 signal transducer and activator of transcription 3 Homo sapiens 101-106 23032719-4 2013 We found that evodiamine suppressed both constitutive and interleukin-6 (IL-6)-induced activation of STAT3 tyrosine 705 (Tyr(705)) effectively. Tyrosine 121-124 signal transducer and activator of transcription 3 Homo sapiens 101-106 23032719-6 2013 Interestingly, treatment of cells with sodium pervanadate abrogated the inhibition of evodiamine on IL-6-induced STAT3 (Tyr(705)) activation indicating the involvement of protein tyrosine phosphatases. Tyrosine 120-123 signal transducer and activator of transcription 3 Homo sapiens 113-118 23032719-8 2013 Moreover, inhibition of SHP-1 gene by small interference RNA abolished the ability of evodiamine to inhibit IL-6-induced STAT3 (Tyr(705)) activation. Tyrosine 128-131 signal transducer and activator of transcription 3 Homo sapiens 121-126 24129709-2 2013 Here we show that KLF4 physically interacts with STAT3 upon cytokine-induced phosphorylation of tyrosine 705 (Y705) on STAT3. Tyrosine 96-104 signal transducer and activator of transcription 3 Homo sapiens 49-54 22746102-9 2013 These effects correlated with the reduction of vascular endothelial growth factor (VEGF), c-myc, and cyclin D1, and could be explained on the basis of inhibition of STAT3 tyrosine phosphorylation. Tyrosine 171-179 signal transducer and activator of transcription 3 Homo sapiens 165-170 24058788-2 2013 Activated STAT3 is phosphorylated on tyrosine and forms a dimer through phosphotyrosine/src homology 2 (SH2) domain interaction. Tyrosine 37-45 signal transducer and activator of transcription 3 Homo sapiens 10-15 23074171-3 2013 We showed that phosphorylation of STAT3 at tyrosine 705 (Y705) and RANTES expression are increased in response to tamoxifen in human breast cancer cells. Tyrosine 43-51 signal transducer and activator of transcription 3 Homo sapiens 34-39 24129709-2 2013 Here we show that KLF4 physically interacts with STAT3 upon cytokine-induced phosphorylation of tyrosine 705 (Y705) on STAT3. Tyrosine 96-104 signal transducer and activator of transcription 3 Homo sapiens 119-124 23125416-0 2012 CCR1-mediated STAT3 tyrosine phosphorylation and CXCL8 expression in THP-1 macrophage-like cells involve pertussis toxin-insensitive Galpha(14/16) signaling and IL-6 release. Tyrosine 20-28 signal transducer and activator of transcription 3 Homo sapiens 14-19 23691485-0 2012 Tyrosine 23 Phosphorylation of Annexin A2 Promotes Proliferation, Invasion, and Stat3 Phosphorylation in the Nucleus of Human Breast Cancer SK-BR-3 Cells. Tyrosine 0-8 signal transducer and activator of transcription 3 Homo sapiens 80-85 23125416-4 2012 In this study, we demonstrated that a CCR1 agonist, leukotactin-1 (CCL15), could indeed stimulate STAT3 Tyr(705) and Ser(727) phosphorylation via pertussis toxin-insensitive G proteins in PMA-differentiated THP-1 cells, human erythroleukemia cells, and HEK293 cells overexpressing CCR1 and Galpha(14/16). Tyrosine 104-107 signal transducer and activator of transcription 3 Homo sapiens 98-103 23125416-5 2012 The STAT3 Tyr(705) and Ser(727) phosphorylations were independent of each other and temporally distinct. Tyrosine 10-13 signal transducer and activator of transcription 3 Homo sapiens 4-9 23125416-6 2012 Subcellular fractionation and confocal microscopy illustrated that Tyr(705)-phosphorylated STAT3 translocated to the nucleus, whereas Ser(727)-phosphorylated STAT3 was retained in the cytosol after CCR1/Galpha(14) activation. Tyrosine 67-70 signal transducer and activator of transcription 3 Homo sapiens 91-96 23125416-8 2012 Neutralizing Ab to IL-6 inhibited CCL15-mediated STAT3 Tyr(705) phosphorylation, whereas inhibition of STAT3 activity abolished CCL15-activated CXCL8 release. Tyrosine 55-58 signal transducer and activator of transcription 3 Homo sapiens 49-54 22922995-7 2012 Immunoblotting showed that IL-22 induced a transient tyrosine phosphorylation of STAT3 (pY705-STAT3) in MISK81-5 cells. Tyrosine 53-61 signal transducer and activator of transcription 3 Homo sapiens 93-98 22922995-7 2012 Immunoblotting showed that IL-22 induced a transient tyrosine phosphorylation of STAT3 (pY705-STAT3) in MISK81-5 cells. Tyrosine 53-61 signal transducer and activator of transcription 3 Homo sapiens 100-111 22961117-6 2012 Inhibition of the signal transducer and activator of transcription 3 tyrosine phosphorylation was found to be achieved through suppression of JAK1 and JAK2. Tyrosine 69-77 signal transducer and activator of transcription 3 Homo sapiens 18-68 22891351-1 2012 Although tyrosine-phosphorylated or activated STAT3 (pY-STAT3) is a well-described mediator of tumorigenesis, its role in thyroid cancer has not been investigated. Tyrosine 9-17 signal transducer and activator of transcription 3 Homo sapiens 46-51 23335526-1 2012 PURPOSE: Signal transducers and activators of transcription (STATs) are tyrosine phosphorylated transcription factors activated by the Jak family kinases. Tyrosine 72-80 signal transducer and activator of transcription 3 Homo sapiens 61-66 22891351-1 2012 Although tyrosine-phosphorylated or activated STAT3 (pY-STAT3) is a well-described mediator of tumorigenesis, its role in thyroid cancer has not been investigated. Tyrosine 9-17 signal transducer and activator of transcription 3 Homo sapiens 56-61 22484976-3 2012 STAT3 also regulates carbonic anhydrase IX (CAIX) expression in vivo, promoting IL-6-dependent tumor invasion.Tumor-tissue from 324 patients with EC and 45 patients with precursor lesions were analyzed for the expressions of tyrosine-705 phosphorylated STAT3 (pSTAT3). Tyrosine 225-233 signal transducer and activator of transcription 3 Homo sapiens 0-5 22465052-5 2012 As Mcl-1 is regulated by signal transducers and activators of transcription 3 (STAT3), we found that SC-2001 downregulated the phosphorylation of STAT3 (Tyr 705) and subsequently inhibited transcriptional activities of STAT3 in a dose-dependent manner. Tyrosine 153-156 signal transducer and activator of transcription 3 Homo sapiens 25-77 22801507-9 2012 BRCA1-mutated cancer cells had higher expression of Tyrosine-phosphorylated STAT3 (pTyr705) as compared with other STAT proteins. Tyrosine 52-60 signal transducer and activator of transcription 3 Homo sapiens 76-81 22824293-9 2012 PEITC treatment substantially decreased the expression of HER2, epidermal growth factor receptor (EGFR) and phosphorylation of signal transducer and activator of transcription 3 (STAT3) at Tyr-705. Tyrosine 189-192 signal transducer and activator of transcription 3 Homo sapiens 127-177 22824293-9 2012 PEITC treatment substantially decreased the expression of HER2, epidermal growth factor receptor (EGFR) and phosphorylation of signal transducer and activator of transcription 3 (STAT3) at Tyr-705. Tyrosine 189-192 signal transducer and activator of transcription 3 Homo sapiens 179-184 22801507-9 2012 BRCA1-mutated cancer cells had higher expression of Tyrosine-phosphorylated STAT3 (pTyr705) as compared with other STAT proteins. Tyrosine 52-60 signal transducer and activator of transcription 3 Homo sapiens 76-80 22197944-5 2012 STAT3 tyrosine phosphorylation (pSTAT3) was measured in circulating leukocytes by flow cytometry, and mechanisms regulating STAT3 activation were tested in IBD Epstein-Barr virus (EBV)-transformed lymphocytes (EBL). Tyrosine 6-14 signal transducer and activator of transcription 3 Homo sapiens 0-5 22197944-9 2012 EBLs from patients carrying the STAT3 "A" risk allele exhibited increased basal and IL-6-stimulated STAT3 tyrosine phosphorylation, increased transcription of STAT3 and SOCS3 after IL-6 stimulation, and increased membrane localization of the IL-6 receptor, GP130, and Janus-associated kinase 2. Tyrosine 106-114 signal transducer and activator of transcription 3 Homo sapiens 32-37 22547065-3 2012 The signal transducer and activator of transcription 3 (STAT3) is one of the substrate targets of Brk, and elevated tyrosine phosphorylation of STAT3 is known to contribute to oncogenesis. Tyrosine 116-124 signal transducer and activator of transcription 3 Homo sapiens 4-54 22566696-3 2012 In addition, STAT-3 is tyrosine-phosphorylated in a biphasic manner, and the late phase requires NFATc1-mediated p300-dependent acetylation. Tyrosine 23-31 signal transducer and activator of transcription 3 Homo sapiens 13-19 22547065-3 2012 The signal transducer and activator of transcription 3 (STAT3) is one of the substrate targets of Brk, and elevated tyrosine phosphorylation of STAT3 is known to contribute to oncogenesis. Tyrosine 116-124 signal transducer and activator of transcription 3 Homo sapiens 56-61 22547065-3 2012 The signal transducer and activator of transcription 3 (STAT3) is one of the substrate targets of Brk, and elevated tyrosine phosphorylation of STAT3 is known to contribute to oncogenesis. Tyrosine 116-124 signal transducer and activator of transcription 3 Homo sapiens 144-149 22261084-2 2012 Signal transduction and activators of transcription 3 (STAT3), a component of the JAK-STAT signaling pathway, can be activated by tyrosine phosphorylation as P-STAT3 and involved in the regulation of cellular growth, survival and oncogenesis. Tyrosine 130-138 signal transducer and activator of transcription 3 Homo sapiens 0-53 22623533-2 2012 BP-1-102 binds Stat3 with an affinity (K(D)) of 504 nM, blocks Stat3-phospho-tyrosine (pTyr) peptide interactions and Stat3 activation at 4-6.8 muM, and selectively inhibits growth, survival, migration, and invasion of Stat3-dependent tumor cells. Tyrosine 77-85 signal transducer and activator of transcription 3 Homo sapiens 63-68 22623533-2 2012 BP-1-102 binds Stat3 with an affinity (K(D)) of 504 nM, blocks Stat3-phospho-tyrosine (pTyr) peptide interactions and Stat3 activation at 4-6.8 muM, and selectively inhibits growth, survival, migration, and invasion of Stat3-dependent tumor cells. Tyrosine 77-85 signal transducer and activator of transcription 3 Homo sapiens 63-68 22623533-2 2012 BP-1-102 binds Stat3 with an affinity (K(D)) of 504 nM, blocks Stat3-phospho-tyrosine (pTyr) peptide interactions and Stat3 activation at 4-6.8 muM, and selectively inhibits growth, survival, migration, and invasion of Stat3-dependent tumor cells. Tyrosine 77-85 signal transducer and activator of transcription 3 Homo sapiens 63-68 22575502-6 2012 Furthermore, resistin induced activation of STAT3 as characterized by increased tyrosine phosphorylation. Tyrosine 80-88 signal transducer and activator of transcription 3 Homo sapiens 44-49 22581261-6 2012 After ligation of TLR9, DOCK8 became tyrosine-phosphorylated by Pyk2, bound the Src-family kinase Lyn and linked TLR9 to a Src-kinase Syk-transcription factor STAT3 cascade essential for TLR9-driven B cell proliferation and differentiation. Tyrosine 37-45 signal transducer and activator of transcription 3 Homo sapiens 159-164 22378781-1 2012 Phosphorylation of signal transducer and activator of transcription 3 (STAT3) on a single tyrosine residue in response to growth factors, cytokines, interferons, and oncogenes activates its dimerization, translocation to the nucleus, binding to the interferon gamma (gamma)-activated sequence (GAS) DNA-binding site and activation of transcription of target genes. Tyrosine 90-98 signal transducer and activator of transcription 3 Homo sapiens 19-69 22378781-1 2012 Phosphorylation of signal transducer and activator of transcription 3 (STAT3) on a single tyrosine residue in response to growth factors, cytokines, interferons, and oncogenes activates its dimerization, translocation to the nucleus, binding to the interferon gamma (gamma)-activated sequence (GAS) DNA-binding site and activation of transcription of target genes. Tyrosine 90-98 signal transducer and activator of transcription 3 Homo sapiens 71-76 21954831-0 2012 Interleukin-6 autocrine signaling mediates melatonin MT(1/2) receptor-induced STAT3 Tyr(705) phosphorylation. Tyrosine 85-88 signal transducer and activator of transcription 3 Homo sapiens 79-84 21954831-2 2012 In the present study, we show that this functional coupling elicited biphasic stimulatory phosphorylation on STAT3 in recombinant MT(1) /Galpha(16) cells and native Jurkat T cells (endogenously expressing MT(1) and Galpha(16) ), with maximal Ser(727) phosphorylation occurring at 15min, while marked Tyr(705) phosphorylation became detectable only upon agonist treatment for 4 hr or more. Tyrosine 300-303 signal transducer and activator of transcription 3 Homo sapiens 109-114 21954831-3 2012 By employing signal transducer and activator of transcription 3 (STAT3) phosphorylation-resistant mutants (STAT3-Y705F and STAT3-S727A), we further showed that the receptor-mediated STAT3 phosphorylations at Ser(727) and Tyr(705) were independent of each other. Tyrosine 221-224 signal transducer and activator of transcription 3 Homo sapiens 13-63 21954831-3 2012 By employing signal transducer and activator of transcription 3 (STAT3) phosphorylation-resistant mutants (STAT3-Y705F and STAT3-S727A), we further showed that the receptor-mediated STAT3 phosphorylations at Ser(727) and Tyr(705) were independent of each other. Tyrosine 221-224 signal transducer and activator of transcription 3 Homo sapiens 65-70 21954831-3 2012 By employing signal transducer and activator of transcription 3 (STAT3) phosphorylation-resistant mutants (STAT3-Y705F and STAT3-S727A), we further showed that the receptor-mediated STAT3 phosphorylations at Ser(727) and Tyr(705) were independent of each other. Tyrosine 221-224 signal transducer and activator of transcription 3 Homo sapiens 107-112 21954831-3 2012 By employing signal transducer and activator of transcription 3 (STAT3) phosphorylation-resistant mutants (STAT3-Y705F and STAT3-S727A), we further showed that the receptor-mediated STAT3 phosphorylations at Ser(727) and Tyr(705) were independent of each other. Tyrosine 221-224 signal transducer and activator of transcription 3 Homo sapiens 107-112 21954831-3 2012 By employing signal transducer and activator of transcription 3 (STAT3) phosphorylation-resistant mutants (STAT3-Y705F and STAT3-S727A), we further showed that the receptor-mediated STAT3 phosphorylations at Ser(727) and Tyr(705) were independent of each other. Tyrosine 221-224 signal transducer and activator of transcription 3 Homo sapiens 107-112 21954831-5 2012 Further experiments revealed that the agonist-induced STAT3 phosphorylation at Tyr(705) was significantly suppressed by pretreatment with cycloheximide (a ribosome inhibitor), suggesting that de novo protein synthesis might play a critical role for this response. Tyrosine 79-82 signal transducer and activator of transcription 3 Homo sapiens 54-59 21954831-7 2012 Antibody against IL-6, but not those for GM-CSF and CXCL-8, effectively abolished the agonist-induced STAT3 Tyr(705) phosphorylation, suggesting the involvement of IL-6 in melatonin receptor-mediated STAT3 activation. Tyrosine 108-111 signal transducer and activator of transcription 3 Homo sapiens 102-107 21954831-8 2012 Our results demonstrate that melatonin receptor/Galpha(16) coupling is capable of triggering the production of cytokines including IL-6, and this autocrine loop may account for the subsequent STAT3 phosphorylation at Tyr(705) . Tyrosine 217-220 signal transducer and activator of transcription 3 Homo sapiens 192-197 22261084-2 2012 Signal transduction and activators of transcription 3 (STAT3), a component of the JAK-STAT signaling pathway, can be activated by tyrosine phosphorylation as P-STAT3 and involved in the regulation of cellular growth, survival and oncogenesis. Tyrosine 130-138 signal transducer and activator of transcription 3 Homo sapiens 55-60 22261084-2 2012 Signal transduction and activators of transcription 3 (STAT3), a component of the JAK-STAT signaling pathway, can be activated by tyrosine phosphorylation as P-STAT3 and involved in the regulation of cellular growth, survival and oncogenesis. Tyrosine 130-138 signal transducer and activator of transcription 3 Homo sapiens 55-59 22261084-2 2012 Signal transduction and activators of transcription 3 (STAT3), a component of the JAK-STAT signaling pathway, can be activated by tyrosine phosphorylation as P-STAT3 and involved in the regulation of cellular growth, survival and oncogenesis. Tyrosine 130-138 signal transducer and activator of transcription 3 Homo sapiens 160-165 22280969-9 2012 Interleukin (IL)-6-induced phosphorylation of STAT3 at Tyr-705 was significantly blocked by DIM. Tyrosine 55-58 signal transducer and activator of transcription 3 Homo sapiens 46-51 22394684-11 2012 The decreased tyrosine phosphorylation of STAT3 is coupled with suppression of STAT3 transcriptional activity and reduced tumor growth in vitro and in vivo. Tyrosine 14-22 signal transducer and activator of transcription 3 Homo sapiens 42-47 22394684-11 2012 The decreased tyrosine phosphorylation of STAT3 is coupled with suppression of STAT3 transcriptional activity and reduced tumor growth in vitro and in vivo. Tyrosine 14-22 signal transducer and activator of transcription 3 Homo sapiens 79-84 22315349-6 2012 Mechanistic studies showed that the antitumor effects of the tyrosine isomers were mediated in part by early inhibition of the MAP/ERK pathway and inactivation of STAT3, potentially driving tumor cells into a state of dormancy in G(0)-phase. Tyrosine 61-69 signal transducer and activator of transcription 3 Homo sapiens 163-168 22374428-0 2012 Tissue microarray-based study of patients with lymph node-positive breast cancer shows tyrosine phosphorylation of signal transducer and activator of transcription 3 (tyrosine705-STAT3) is a marker of good prognosis. Tyrosine 87-95 signal transducer and activator of transcription 3 Homo sapiens 115-165 22374428-0 2012 Tissue microarray-based study of patients with lymph node-positive breast cancer shows tyrosine phosphorylation of signal transducer and activator of transcription 3 (tyrosine705-STAT3) is a marker of good prognosis. Tyrosine 87-95 signal transducer and activator of transcription 3 Homo sapiens 179-184 22280969-7 2012 Phosphorylation of STAT3 at Tyr-705 and Ser-727 was reduced by DIM in a concentration-dependent manner. Tyrosine 28-31 signal transducer and activator of transcription 3 Homo sapiens 19-24 22366233-2 2012 We report that treatment of human microvascular endothelial cells with sodium selenate at a pharmacological dose (100 muM) enhanced tyrosine phosphorylation of nuclear STAT3 on Y705 in response to IL-6-type cytokine, leukemia inhibitory factor (LIF), indicative of enhanced STAT3 activity. Tyrosine 132-140 signal transducer and activator of transcription 3 Homo sapiens 168-173 22394684-10 2012 Over-expression of PTPMeg2 decreased tyrosine phosphorylation of STAT3 while depletion of PTPMeg2 increased its phosphorylation. Tyrosine 37-45 signal transducer and activator of transcription 3 Homo sapiens 65-70 22180308-4 2012 Dovitinib downregulated phospho-STAT3 (p-STAT3) at tyrosine 705 and subsequently reduced the levels of expression of STAT3-related proteins Mcl-1, survivin, and cyclin D1 in a time-dependent manner. Tyrosine 51-59 signal transducer and activator of transcription 3 Homo sapiens 32-37 22180308-4 2012 Dovitinib downregulated phospho-STAT3 (p-STAT3) at tyrosine 705 and subsequently reduced the levels of expression of STAT3-related proteins Mcl-1, survivin, and cyclin D1 in a time-dependent manner. Tyrosine 51-59 signal transducer and activator of transcription 3 Homo sapiens 41-46 22180308-4 2012 Dovitinib downregulated phospho-STAT3 (p-STAT3) at tyrosine 705 and subsequently reduced the levels of expression of STAT3-related proteins Mcl-1, survivin, and cyclin D1 in a time-dependent manner. Tyrosine 51-59 signal transducer and activator of transcription 3 Homo sapiens 41-46 21385272-6 2011 Silencing efficiency and STAT3 tyrosine phosphorylation have been analyzed by Western blotting. Tyrosine 31-39 signal transducer and activator of transcription 3 Homo sapiens 25-30 23189171-10 2012 CCHCR1 also regulates EGF-induced STAT3 activation in an isoform-specific manner: the tyrosine phosphorylation of STAT3 is disturbed in isoform 3-transfected cells. Tyrosine 86-94 signal transducer and activator of transcription 3 Homo sapiens 34-39 23189171-10 2012 CCHCR1 also regulates EGF-induced STAT3 activation in an isoform-specific manner: the tyrosine phosphorylation of STAT3 is disturbed in isoform 3-transfected cells. Tyrosine 86-94 signal transducer and activator of transcription 3 Homo sapiens 114-119 22315522-3 2012 STAT3 was activated (tyrosine 705-phosphorylated) in 18 out of 31 primary tumours (58%), either diffusely (35%) or focally (23%). Tyrosine 21-29 signal transducer and activator of transcription 3 Homo sapiens 0-5 21685938-4 2012 Our results indicate that HA binding to CD44 promotes Nanog-Stat-3 (also tyrosine phosphorylated Stat-3) complex formation, nuclear translocation and transcriptional activation. Tyrosine 73-81 signal transducer and activator of transcription 3 Homo sapiens 60-66 21685938-4 2012 Our results indicate that HA binding to CD44 promotes Nanog-Stat-3 (also tyrosine phosphorylated Stat-3) complex formation, nuclear translocation and transcriptional activation. Tyrosine 73-81 signal transducer and activator of transcription 3 Homo sapiens 97-103 23144154-6 2012 Induction required the shared receptor subunit gp130, and correlated with increased tyrosine phosphorylation of STAT3. Tyrosine 84-92 signal transducer and activator of transcription 3 Homo sapiens 112-117 23166634-7 2012 In addition, SOID-8 reduced tyrosine phosphorylation of Signal Tansducer and Activator of Transcription 3 (STAT3) in both dose- and time-dependent manners. Tyrosine 28-36 signal transducer and activator of transcription 3 Homo sapiens 56-105 23166634-7 2012 In addition, SOID-8 reduced tyrosine phosphorylation of Signal Tansducer and Activator of Transcription 3 (STAT3) in both dose- and time-dependent manners. Tyrosine 28-36 signal transducer and activator of transcription 3 Homo sapiens 107-112 22084446-6 2011 Mechanistic studies showed that the antitumor effects of the tyrosine isoforms were mediated, in part, by early inhibition of mitogen-activated protein/extracellular signal-regulated kinase pathway and inactivation of STAT3, potentially driving tumor cells into a state of dormancy. Tyrosine 61-69 signal transducer and activator of transcription 3 Homo sapiens 218-223 22110204-5 2011 Down-regulation of p38 MAPK expression levels by siRNA strongly reduces phosphorylation of STAT3 tyrosine 705 without any effects on phosphorylation of STAT3 serine 727 and STAT1. Tyrosine 97-105 signal transducer and activator of transcription 3 Homo sapiens 91-96 22006329-5 2011 Induction of endogenous STAT3beta leads to apoptosis and cell-cycle arrest in cell lines with persistent STAT3 tyrosine phosphorylation compared with total STAT3 knockdown obtained by forced splicing-dependent nonsense-mediated decay (FSD-NMD). Tyrosine 111-119 signal transducer and activator of transcription 3 Homo sapiens 24-29 22006329-5 2011 Induction of endogenous STAT3beta leads to apoptosis and cell-cycle arrest in cell lines with persistent STAT3 tyrosine phosphorylation compared with total STAT3 knockdown obtained by forced splicing-dependent nonsense-mediated decay (FSD-NMD). Tyrosine 111-119 signal transducer and activator of transcription 3 Homo sapiens 105-110 21385272-11 2011 SOCS3 silencing induced increase in STAT3 tyrosine phosphorylation and cytotoxicity. Tyrosine 42-50 signal transducer and activator of transcription 3 Homo sapiens 36-41 21325634-7 2011 Second, curcumin suppressed activation of signal transducer and activator of transcription-3 as indicated by decreased phosphorylation at both tyrosine(705) and serine(727) and inhibition of janus kinase-1 phosphorylation. Tyrosine 143-151 signal transducer and activator of transcription 3 Homo sapiens 42-92 21788516-1 2011 Cells transformed by the p110alpha-H1047R mutant of PI3K show increased tyrosine phosphorylation of Stat3. Tyrosine 72-80 signal transducer and activator of transcription 3 Homo sapiens 100-105 21673103-8 2011 Visfatin alone increased the transcriptional activity and tyrosine phosphorylation of STAT3, which was suppressed by Janus kinase (JAK) 2 inhibitor. Tyrosine 58-66 signal transducer and activator of transcription 3 Homo sapiens 86-91 21061196-6 2011 Using this method, we studied the distribution of tyrosine phosphorylated signal transducer and activator of transcription 3 (PY-STAT3) in LV tissue of animals treated with the beta-agonist dobutamine. Tyrosine 50-58 signal transducer and activator of transcription 3 Homo sapiens 74-124 21619876-5 2011 Specifically, Rac1, acting in a complex with the MgcRacGAP (male germ cell RacGAP), promotes tyrosine phosphorylation of Stat3 by the IL6-receptor family/Jak kinase complex, as well as its translocation to the nucleus. Tyrosine 93-101 signal transducer and activator of transcription 3 Homo sapiens 121-126 21690253-7 2011 This was associated with phosphorylation of tyrosine 705, a residue required for IL-6-induced STAT3 activation. Tyrosine 44-52 signal transducer and activator of transcription 3 Homo sapiens 94-99 21490133-4 2011 STAT3 phosphorylation at both tyrosine residue 705 and serine residue 727 was inhibited by GA. Tyrosine 30-38 signal transducer and activator of transcription 3 Homo sapiens 0-5 21386060-4 2011 Although both IFNs stimulated phosphorylation of STAT1 (at Tyr(701)) and STAT3 (at Tyr(705)) after treatment for 30 min, subsequent properties of induction of the SOCS isoform were different. Tyrosine 83-86 signal transducer and activator of transcription 3 Homo sapiens 73-78 21622220-1 2011 Persistent tyrosine phosphorylation of Stat3 and Stat5 is associated with oncogenic activity. Tyrosine 11-19 signal transducer and activator of transcription 3 Homo sapiens 39-44 21402692-7 2011 Stat3 (Tyr-705) phosphorylation was inhibited by CYP3A4 silencing, providing a potential mechanism for CYP3A4 involvement in breast cancer cell growth. Tyrosine 7-10 signal transducer and activator of transcription 3 Homo sapiens 0-5 21499010-5 2011 Consequently, BOT-4-one significantly inhibited STAT3 tyrosine phosphorylation and expression of STAT3 downstream target gene SOCS3 in various human cancer cell lines, and its effect was more potent in JAK3-activated Hodgkin"s lymphoma cell line than in JAK2-activated breast cancer and prostate cancer cell lines. Tyrosine 54-62 signal transducer and activator of transcription 3 Homo sapiens 48-53 21369699-7 2011 Notably, Tyr-705-phosphorylated STAT3 up-regulation was not significantly suppressed by STX-0119, as opposed to other STAT3 inhibitors. Tyrosine 9-12 signal transducer and activator of transcription 3 Homo sapiens 32-37 21518865-3 2011 Here we show that membrane-anchored PC1 activates STAT3 in a JAK2-dependent manner, leading to tyrosine phosphorylation and transcriptional activity. Tyrosine 95-103 signal transducer and activator of transcription 3 Homo sapiens 50-55 20824812-9 2011 RESULTS: IL-22 treatment enhanced the expression of DMBT1 through STAT3 tyrosine phosphorylation and NF-kappaB activation in colon cancer cells. Tyrosine 72-80 signal transducer and activator of transcription 3 Homo sapiens 66-71 21430070-6 2011 Importantly, AT9283 also inhibited STAT3 tyrosine phosphorylation in MM cells. Tyrosine 41-49 signal transducer and activator of transcription 3 Homo sapiens 35-40 21107604-5 2011 Genetic study revealed a heterozygous mutation in exon 21 of STAT3 gene (g.66583 A > C, c.1970A > C) in the boy, which resulted in a substitution of tyrosine at the amino acid position 657 to serine (p.Y657S) in the Src homology 2 (SH2) domain of STAT3. Tyrosine 155-163 signal transducer and activator of transcription 3 Homo sapiens 61-66 21107604-5 2011 Genetic study revealed a heterozygous mutation in exon 21 of STAT3 gene (g.66583 A > C, c.1970A > C) in the boy, which resulted in a substitution of tyrosine at the amino acid position 657 to serine (p.Y657S) in the Src homology 2 (SH2) domain of STAT3. Tyrosine 155-163 signal transducer and activator of transcription 3 Homo sapiens 253-258 21915376-6 2011 On further examination, intracellular Ang II was found to activate Stat3 transcription factor including increased Stat3 protein expression, tyrosine 705 phosphorylation, and DNA-binding activity. Tyrosine 140-148 signal transducer and activator of transcription 3 Homo sapiens 67-72 20830487-7 2011 RESULTS: Forced aggregation and non-adhesive growth of HT-29 CRC cells resulted in enhanced tyrosine phosphorylation of STAT3 and elevated expression of matrix metalloproteinase MMP-1. Tyrosine 92-100 signal transducer and activator of transcription 3 Homo sapiens 120-125 21307189-5 2011 Upon treatment with EGF, the LMP1-CTAR1-induced EGFR was additionally phosphorylated and STAT3 became phosphorylated on tyrosine, concomitant with upregulation of a subset of STAT3 target genes. Tyrosine 120-128 signal transducer and activator of transcription 3 Homo sapiens 89-94 21216930-5 2011 Inhibition of JAK1 with small molecules or RNA interference resulted in loss of STAT3 tyrosine phosphorylation and inhibition of cell growth. Tyrosine 86-94 signal transducer and activator of transcription 3 Homo sapiens 80-85 21216930-6 2011 An interleukin-6 neutralizing antibody, siltuximab (CNTO 328) could inhibit STAT3 tyrosine phosphorylation in a cell-dependent manner. Tyrosine 82-90 signal transducer and activator of transcription 3 Homo sapiens 76-81 21216930-7 2011 Siltuximab could completely inhibit STAT3 tyrosine phosphorylation in H1650 cells, and this resulted in inhibition of lung cancer cell growth in vivo. Tyrosine 42-50 signal transducer and activator of transcription 3 Homo sapiens 36-41 20888416-6 2011 The active inhibitors also attenuated IFN-gamma-induced phosphorylation of Tyr(705)-STAT3 and astrocytic expression of intercellular adhesion molecule-1 (ICAM-1). Tyrosine 75-78 signal transducer and activator of transcription 3 Homo sapiens 84-89 21486470-3 2011 Cytoplasmic STAT3 dimers are activated by tyrosine phosphorylation; they interact with importins via a nuclear localization signal (NLS) one of which is located within the DNA-binding domain formed by the dimer. Tyrosine 42-50 signal transducer and activator of transcription 3 Homo sapiens 12-17 21325026-2 2011 In response to cytokine stimulation, STAT3 becomes phosphorylated on a single tyrosine residue. Tyrosine 78-86 signal transducer and activator of transcription 3 Homo sapiens 37-42 21325026-3 2011 Tyrosine-phosphorylated STAT3 accumulates in the nucleus, binds to specific DNA response elements and induces gene expression. Tyrosine 0-8 signal transducer and activator of transcription 3 Homo sapiens 24-29 21098725-2 2011 The IL-22-induced effects are mediated by STAT3, whose activity is proportional to acetylation in lysine (Lys)685 and phosphorylation in tyrosine (Tyr)705. Tyrosine 137-145 signal transducer and activator of transcription 3 Homo sapiens 42-47 21098725-2 2011 The IL-22-induced effects are mediated by STAT3, whose activity is proportional to acetylation in lysine (Lys)685 and phosphorylation in tyrosine (Tyr)705. Tyrosine 147-150 signal transducer and activator of transcription 3 Homo sapiens 42-47 21568920-7 2011 In contrast, in many cancerous cell lines and tumors, where growth factor dysregulation is frequently at the heart of cellular transformation, the STAT proteins (in particular STAT1, 3 and 5) are persistently tyrosine-phosphorylated or activated; abnormal levels of STAT3 activation have been observed in breast, ovarian, prostate, hematological and head and neck cancer cell lines. Tyrosine 209-217 signal transducer and activator of transcription 3 Homo sapiens 266-271 21915376-8 2011 In contrast, extracellular Ang II-induced tyrosine 705 phosphorylation of Stat3 was inhibited by AG-490 confirming the presence of a Jak2-dependent pathway. Tyrosine 42-50 signal transducer and activator of transcription 3 Homo sapiens 74-79 22140473-1 2011 We determined that signal transducer and activator of transcription 3 (Stat3) is tyrosine phosphorylated in 37% of primary breast tumors and 63% of paired metastatic axillary lymph nodes. Tyrosine 81-89 signal transducer and activator of transcription 3 Homo sapiens 19-69 20978825-3 2011 In response to IL-6, the signal transducer and activator of transcription 3 (STAT3) becomes phosphorylated on tyrosine and serine residues. Tyrosine 110-118 signal transducer and activator of transcription 3 Homo sapiens 25-75 20978825-3 2011 In response to IL-6, the signal transducer and activator of transcription 3 (STAT3) becomes phosphorylated on tyrosine and serine residues. Tyrosine 110-118 signal transducer and activator of transcription 3 Homo sapiens 77-82 20978825-9 2011 Preincubating HepG(2) cells with adiponectin led to a decline in STAT3 phosphorylation on both tyrosine and serine residues. Tyrosine 95-103 signal transducer and activator of transcription 3 Homo sapiens 65-70 21949561-5 2011 We found that microtubule stabilizers, such as paclitaxel, or microtubule inhibitors, such as vinorelbine, decrease the activating tyrosine phosphorylation of STAT3 in tumor cells and inhibit the expression of STAT3 target genes. Tyrosine 131-139 signal transducer and activator of transcription 3 Homo sapiens 159-164 22140473-1 2011 We determined that signal transducer and activator of transcription 3 (Stat3) is tyrosine phosphorylated in 37% of primary breast tumors and 63% of paired metastatic axillary lymph nodes. Tyrosine 81-89 signal transducer and activator of transcription 3 Homo sapiens 71-76 22016776-12 2011 Immunoblotting of BITC treated tumors show reduced expression of STAT-3 phosphorylation (Tyr-705), HIF-alpha, VEGFR-2, VEGF, MMP-2, CD31 and RhoC. Tyrosine 89-92 signal transducer and activator of transcription 3 Homo sapiens 65-71 20691278-6 2010 Specifically, we reveal that JAK2 mediates STAT3 tyrosine phosphorylation in response to the two BMPs, whereas BMP2- and BMP4-induced STAT3 serine phosphorylation involves two divergent cascades, namely the mTOR and ERK1/2 cascades, respectively. Tyrosine 49-57 signal transducer and activator of transcription 3 Homo sapiens 43-48 21625597-2 2011 We report that, in human THP-1 cell line, they inhibit IL-6-elicited tyrosine phosphorylation of STAT3 and its DNA binding activity with EC(50) of 10 microM with concomitant down-regulation of the phosphorylation of the tyrosine Janus kinases JAK1, JAK2 and Tyk2. Tyrosine 69-77 signal transducer and activator of transcription 3 Homo sapiens 97-102 21625597-5 2011 Finally, the glutathione ethylene ester (GEE), the cell permeable GSH form, reverts the inhibitory action of DCE and CS on STAT3 tyrosine phosphorylation. Tyrosine 129-137 signal transducer and activator of transcription 3 Homo sapiens 123-128 20929863-7 2010 The PH domain of STAP-2 is involved in multiple steps: the binding between Brk and STAP-2, the activation and tyrosine phosphorylation of STAT3, and the activation of Brk. Tyrosine 110-118 signal transducer and activator of transcription 3 Homo sapiens 138-143 20929863-8 2010 Notably, a STAP-2 PH-Brk fusion protein exhibited robust kinase activity and increased activation and tyrosine phosphorylation of STAT3. Tyrosine 102-110 signal transducer and activator of transcription 3 Homo sapiens 130-135 20455003-0 2010 STAT3 tyrosine phosphorylation influences survival in glioblastoma. Tyrosine 6-14 signal transducer and activator of transcription 3 Homo sapiens 0-5 20724373-3 2010 The WA treatment (2 and 4 muM) decreased constitutive (MDA-MB-231) and/or IL-6-inducible (MDA-MB-231 and MCF-7) phosphorylation of STAT3 (Tyr(705)) and its upstream regulator Janus-activated kinase 2 (JAK2; Tyr(1007/1008)) in MDA-MB-231, which was accompanied by suppression of their protein levels especially at the higher concentration. Tyrosine 138-141 signal transducer and activator of transcription 3 Homo sapiens 131-136 20724373-3 2010 The WA treatment (2 and 4 muM) decreased constitutive (MDA-MB-231) and/or IL-6-inducible (MDA-MB-231 and MCF-7) phosphorylation of STAT3 (Tyr(705)) and its upstream regulator Janus-activated kinase 2 (JAK2; Tyr(1007/1008)) in MDA-MB-231, which was accompanied by suppression of their protein levels especially at the higher concentration. Tyrosine 207-210 signal transducer and activator of transcription 3 Homo sapiens 131-136 20724373-4 2010 Exposure of MDA-MB-231 or MCF-7 cells to WA also resulted in suppression of (i) transcriptional activity of STAT3 with or without IL-6 stimulation in both cells; (ii) dimerization of STAT3 (MDA-MB-231) and (iii) nuclear translocation of Tyr(705)-phosphorylated STAT3 in both cells. Tyrosine 237-240 signal transducer and activator of transcription 3 Homo sapiens 183-188 20724373-4 2010 Exposure of MDA-MB-231 or MCF-7 cells to WA also resulted in suppression of (i) transcriptional activity of STAT3 with or without IL-6 stimulation in both cells; (ii) dimerization of STAT3 (MDA-MB-231) and (iii) nuclear translocation of Tyr(705)-phosphorylated STAT3 in both cells. Tyrosine 237-240 signal transducer and activator of transcription 3 Homo sapiens 183-188 21625522-2 2011 Cytokines such as interleukin-6 and interferon stimulate the specific tyrosine phosphorylation of STAT3, which confers its ability to bind consensus DNA targets. Tyrosine 70-78 signal transducer and activator of transcription 3 Homo sapiens 98-103 21098664-1 2010 Following its tyrosine phosphorylation, STAT3 is methylated on K140 by the histone methyl transferase SET9 and demethylated by LSD1 when it is bound to a subset of the promoters that it activates. Tyrosine 14-22 signal transducer and activator of transcription 3 Homo sapiens 40-45 21084727-5 2010 The induction of aerobic glycolysis is an important component of STAT3 pro-oncogenic activities, since inhibition of STAT3 tyrosine phosphorylation in the tumour cell lines down-regulates glycolysis prior to leading to growth arrest and cell death, both in vitro and in vivo. Tyrosine 123-131 signal transducer and activator of transcription 3 Homo sapiens 65-70 21084727-5 2010 The induction of aerobic glycolysis is an important component of STAT3 pro-oncogenic activities, since inhibition of STAT3 tyrosine phosphorylation in the tumour cell lines down-regulates glycolysis prior to leading to growth arrest and cell death, both in vitro and in vivo. Tyrosine 123-131 signal transducer and activator of transcription 3 Homo sapiens 117-122 20881637-15 2010 Tyrosine phosphorylated STAT3 was abundant in the surgically resected tumor tissue, indicating potential role in primary resistance in vivo. Tyrosine 0-8 signal transducer and activator of transcription 3 Homo sapiens 24-29 20693278-7 2010 The loss of STAT3 tyrosine phosphorylation is also reflected in an inhibition of expression of STAT3 target genes. Tyrosine 18-26 signal transducer and activator of transcription 3 Homo sapiens 12-17 20693278-7 2010 The loss of STAT3 tyrosine phosphorylation is also reflected in an inhibition of expression of STAT3 target genes. Tyrosine 18-26 signal transducer and activator of transcription 3 Homo sapiens 95-100 20828402-7 2010 Activation of JAKs and STAT3 proteins was evaluated by western blotting for specific tyrosine phosphorylation. Tyrosine 85-93 signal transducer and activator of transcription 3 Homo sapiens 23-28 20693312-8 2010 Whereas IL-6 stimulated tyrosine phosphorylation of STAT3 in a time- and dose-dependent way, DXM and BTM had no effect on STAT3 phosphorylation. Tyrosine 24-32 signal transducer and activator of transcription 3 Homo sapiens 52-57 20516069-2 2010 Following tyrosine phosphorylation, STAT3 proteins dimerize, translocate to the nucleus, and activate specific target genes involved in cell-cycle progression. Tyrosine 10-18 signal transducer and activator of transcription 3 Homo sapiens 36-41 20706987-3 2010 Bacterial endotoxin induced STAT3 tyrosine phosphorylation in macrophages. Tyrosine 34-42 signal transducer and activator of transcription 3 Homo sapiens 28-33 20706987-9 2010 In vivo, inhibition of STAT3 tyrosine phosphorylation by stattic prevented systemic inflammation and improved survival in experimental sepsis. Tyrosine 29-37 signal transducer and activator of transcription 3 Homo sapiens 23-28 20706987-11 2010 These results reveal the immunological implications of tyrosine-unphosphorylated STAT3 in infectious diseases. Tyrosine 55-63 signal transducer and activator of transcription 3 Homo sapiens 81-86 20484629-9 2010 Finally, increased tyrosine phosphorylation of STAT3 is also observed in postmortem brains of AD patients. Tyrosine 19-27 signal transducer and activator of transcription 3 Homo sapiens 47-52 20470863-7 2010 Furthermore, intracranial growth inhibition by sorafenib was accompanied by a significant reduction in ph-Stat3 (Tyr 705) levels. Tyrosine 113-116 signal transducer and activator of transcription 3 Homo sapiens 106-111 19876927-4 2010 Upon leptin binding to its receptor Ob-RL (obesity receptor), STAT3 tyrosine phosphorylation and transactivation activity were enhanced by simultaneously expressing ERalpha. Tyrosine 68-76 signal transducer and activator of transcription 3 Homo sapiens 62-67 20153296-9 2010 We also found that flavonoids suppressed hypoxia-induced STAT3 tyrosine phosphorylation and that this activity correlated with their potency as VEGF inhibitors, suggesting that inhibition of STAT3 function may play a role in this process. Tyrosine 63-71 signal transducer and activator of transcription 3 Homo sapiens 57-62 20153296-9 2010 We also found that flavonoids suppressed hypoxia-induced STAT3 tyrosine phosphorylation and that this activity correlated with their potency as VEGF inhibitors, suggesting that inhibition of STAT3 function may play a role in this process. Tyrosine 63-71 signal transducer and activator of transcription 3 Homo sapiens 191-196 20353755-4 2010 In our present studies, we demonstrate that NP-induced activation of STAT3 tyrosine phosphorylation is independent of leukemia inhibitory factor receptor (LIFR) phosphorylation and degradation. Tyrosine 75-83 signal transducer and activator of transcription 3 Homo sapiens 69-74 20154216-2 2010 Constitutive tyrosine phosphorylation (p) of signal transducer and activator of transcription (STAT) 3 occurs in several solid tumors and hematologic malignancies. Tyrosine 13-21 signal transducer and activator of transcription 3 Homo sapiens 45-102 20388777-3 2010 Using a proteomics approach, we identified signal transducers and activators of transcription-3 (STAT3) as a receptor-binding partner that is mediated by Tyr(677) phosphorylation on FGFR. Tyrosine 154-157 signal transducer and activator of transcription 3 Homo sapiens 43-95 20388777-3 2010 Using a proteomics approach, we identified signal transducers and activators of transcription-3 (STAT3) as a receptor-binding partner that is mediated by Tyr(677) phosphorylation on FGFR. Tyrosine 154-157 signal transducer and activator of transcription 3 Homo sapiens 97-102 20388777-5 2010 Tyrosine phosphorylation of STAT3 was also dependent on concomitant FGFR-dependent activity of SRC and JAK kinases. Tyrosine 0-8 signal transducer and activator of transcription 3 Homo sapiens 28-33 20388777-6 2010 Lastly, tyrosine (but not serine) phosphorylation of STAT3 required amplified FGFR protein expression, generated either by enforced overexpression or as associated with gene amplification in cancer cells. Tyrosine 8-16 signal transducer and activator of transcription 3 Homo sapiens 53-58 20154216-3 2010 In CLL, however, STAT3 is constitutively phosphorylated on serine 727, not tyrosine 705, residues. Tyrosine 75-83 signal transducer and activator of transcription 3 Homo sapiens 17-22 20154216-6 2010 Dephosphorylation of inducible tyrosine pSTAT3 did not affect STAT3-DNA binding, suggesting that constitutive serine pSTAT3 binds DNA. Tyrosine 31-39 signal transducer and activator of transcription 3 Homo sapiens 41-46 20233902-4 2010 Growth-suppressive concentrations of SFN (20 and 40 micromol/L) decreased constitutive (DU145 cells) and IL-6-induced (DU145 and LNCaP cells) phosphorylation of STAT3 (Tyr(705)) as well as its upstream regulator Janus-activated kinase 2 (Tyr(1007/1008)). Tyrosine 168-171 signal transducer and activator of transcription 3 Homo sapiens 161-166 20233902-4 2010 Growth-suppressive concentrations of SFN (20 and 40 micromol/L) decreased constitutive (DU145 cells) and IL-6-induced (DU145 and LNCaP cells) phosphorylation of STAT3 (Tyr(705)) as well as its upstream regulator Janus-activated kinase 2 (Tyr(1007/1008)). Tyrosine 238-241 signal transducer and activator of transcription 3 Homo sapiens 161-166 20401565-6 2010 RESULTS: H(2)O(2) induced phosphorylation of Tyr-705 STAT3, JAK2, and ERK1/2 in LECs. Tyrosine 45-48 signal transducer and activator of transcription 3 Homo sapiens 53-58 19965640-8 2010 It also inhibited activation-induced tyrosine phosphorylation of signal transducer and activator of transcription-3 (STAT-3), and inhibited the transcription of IL-10. Tyrosine 37-45 signal transducer and activator of transcription 3 Homo sapiens 65-115 19965640-8 2010 It also inhibited activation-induced tyrosine phosphorylation of signal transducer and activator of transcription-3 (STAT-3), and inhibited the transcription of IL-10. Tyrosine 37-45 signal transducer and activator of transcription 3 Homo sapiens 117-123 20065946-9 2010 IL-22 simulation enhanced the expression of REG Ialpha protein through STAT3 tyrosine phosphorylation in colon cancer cells. Tyrosine 77-85 signal transducer and activator of transcription 3 Homo sapiens 71-76 20401565-7 2010 In cells pretreated with both AG490 and U0126, phosphorylation of Tyr-705 STAT3, JAK2, and ERK1/2 was suppressed. Tyrosine 66-69 signal transducer and activator of transcription 3 Homo sapiens 74-79 19995546-3 2010 Upon depletion of ERp57 by RNA interference, the phosphorylation of STAT3 on tyrosine 705 was decreased, and the IL-6-induced activation of CRP expression was completely suppressed. Tyrosine 77-85 signal transducer and activator of transcription 3 Homo sapiens 68-73 20197401-8 2010 Sorafenib inhibited constitutive STAT3 phosphorylation (Tyr(705)) and suppressed Mcl-1 and Bcl-x(L) proteins in a dose- and time-dependent manner. Tyrosine 56-59 signal transducer and activator of transcription 3 Homo sapiens 33-38 20128024-1 2010 AIM: To explore the relationship between Cripto-1 (CR-1) and tyrosine phosphorylation STAT3 (p-STAT3) expressions in gastric cancer (GC) and gastric carcinogensis and metastasis. Tyrosine 61-69 signal transducer and activator of transcription 3 Homo sapiens 86-91 20128024-1 2010 AIM: To explore the relationship between Cripto-1 (CR-1) and tyrosine phosphorylation STAT3 (p-STAT3) expressions in gastric cancer (GC) and gastric carcinogensis and metastasis. Tyrosine 61-69 signal transducer and activator of transcription 3 Homo sapiens 95-100 20929542-1 2010 INTRODUCTION: Tyrosine phosphorylated signal transducer and activator of transcription 3 (pStat3) is present in numerous cancers and is required for mediating tumorigenesis. Tyrosine 14-22 signal transducer and activator of transcription 3 Homo sapiens 38-88 20929542-4 2010 Recently, however non-tyrosine phosphorylated Stat3 was shown to have a transcriptional activating function, a role in mitochondrial function and to mediate cell migration.. Tyrosine 22-30 signal transducer and activator of transcription 3 Homo sapiens 46-51 20851386-6 2010 Western blot analysis of STAT3 phosphorylation showed that this augmented response in HT-29 cells following IL-10 neutralization is probably mediated through enhanced IL-6-induced phosphorylation (Tyr(705)) of STAT3 proteins. Tyrosine 197-200 signal transducer and activator of transcription 3 Homo sapiens 25-30 20851386-6 2010 Western blot analysis of STAT3 phosphorylation showed that this augmented response in HT-29 cells following IL-10 neutralization is probably mediated through enhanced IL-6-induced phosphorylation (Tyr(705)) of STAT3 proteins. Tyrosine 197-200 signal transducer and activator of transcription 3 Homo sapiens 210-215 20053726-7 2010 Dephosphorylation of STAT3 (Tyr(705)) induced by sunitinib was helped by a reduction in activities of Janus-activated kinase 2 and Src. Tyrosine 28-31 signal transducer and activator of transcription 3 Homo sapiens 21-26 19632985-0 2009 New activation modus of STAT3: a tyrosine-less region of the interleukin-22 receptor recruits STAT3 by interacting with its coiled-coil domain. Tyrosine 33-41 signal transducer and activator of transcription 3 Homo sapiens 24-29 19875458-12 2009 In conclusion, our study reveals STAT3 as a novel mediator of nutrient signals and identifies a Ser(727) phosphorylation-dependent and Tyr(705) phosphorylation-independent STAT3 activation mechanism in the modulation of insulin signaling. Tyrosine 135-138 signal transducer and activator of transcription 3 Homo sapiens 172-177 19821497-3 2009 Sorafenib treatment of three human CCA cell lines resulted in Tyr(705) phospho-STAT3 dephosphorylation. Tyrosine 62-65 signal transducer and activator of transcription 3 Homo sapiens 79-84 19821497-4 2009 Similar results were obtained with the Raf-kinase inhibitor ZM336372, suggesting sorafenib promotes Tyr(705) phospho-STAT3 dephosphorylation by inhibiting Raf-kinase activity. Tyrosine 100-103 signal transducer and activator of transcription 3 Homo sapiens 117-122 19821497-6 2009 Consistent with this observation, small interfering RNA-mediated knockdown of phosphatase shatterproof 2 (SHP2) inhibited sorafenib-induced Tyr(705) phospho-STAT3 dephosphorylation. Tyrosine 140-143 signal transducer and activator of transcription 3 Homo sapiens 157-162 19751774-4 2009 Here, we found that visfatin induced the activation of STAT3, as characterized by increased tyrosine phosphorylation, nuclear translocation, and DNA-binding activity in human endothelial cells. Tyrosine 92-100 signal transducer and activator of transcription 3 Homo sapiens 55-60 20149042-1 2009 AIM: To investigate the expressions of signal transducer and activator of transcription 3 (Stat3) and tyrosine-activated Stat3 (p-Stat3) in epithelial ovarian carcinoma (EOC) and their relationships with clinical pathological parameters or prognosis. Tyrosine 102-110 signal transducer and activator of transcription 3 Homo sapiens 121-126 20149042-1 2009 AIM: To investigate the expressions of signal transducer and activator of transcription 3 (Stat3) and tyrosine-activated Stat3 (p-Stat3) in epithelial ovarian carcinoma (EOC) and their relationships with clinical pathological parameters or prognosis. Tyrosine 102-110 signal transducer and activator of transcription 3 Homo sapiens 128-135 19808903-7 2009 The induction of HES1 enhanced STAT3 phosphorylation at Tyr 705 as well as SRC phosphorylation at Tyr 416 in inducible HeLa/rtTAA/TRE-HES1 cells, which express HES1 in response to doxycycline treatment. Tyrosine 56-59 signal transducer and activator of transcription 3 Homo sapiens 31-36 19797267-1 2009 Tyrosine phosphorylation of signal transducers and activators of transcription (STATs) promotes their dimerization and ability to bind target genes in the nucleus. Tyrosine 0-8 signal transducer and activator of transcription 3 Homo sapiens 80-85 19632985-0 2009 New activation modus of STAT3: a tyrosine-less region of the interleukin-22 receptor recruits STAT3 by interacting with its coiled-coil domain. Tyrosine 33-41 signal transducer and activator of transcription 3 Homo sapiens 94-99 19632985-2 2009 Here, we show that the C terminus of the interleukin-22 receptor (IL-22R) recruits in a tyrosine-independent manner the coiled-coil domain of STAT3. Tyrosine 88-96 signal transducer and activator of transcription 3 Homo sapiens 142-147 19632985-4 2009 Coimmunoprecipitation and glutathione S-transferase pulldown experiments showed that the coiled-coil domain of STAT3 is constitutively associated with the C-terminal part of IL-22R, and a chimeric STAT3-STAT5 protein containing the coiled-coil domain of STAT3 could be activated by this tyrosine-independent mechanism. Tyrosine 287-295 signal transducer and activator of transcription 3 Homo sapiens 111-116 19509418-5 2009 Biochemical and functional analyses demonstrate that activated Shp2 is able to down-regulate Tyr(P)-Stat3 and that constitutively active Stat3 rescues activating mutant Shp2-induced granulocyte-macrophage colony-stimulating factor hypersensitivity in bone marrow cells. Tyrosine 93-96 signal transducer and activator of transcription 3 Homo sapiens 100-105 19633423-6 2009 Mirk knockdown by small interfering RNA induced cell apoptosis concomitant with upregulation of Bak, a Bcl-2 family member, and downregulation of signal transducers and activators of transcription 3 (STAT3) tyrosine phosphorylation. Tyrosine 207-215 signal transducer and activator of transcription 3 Homo sapiens 146-198 19633423-6 2009 Mirk knockdown by small interfering RNA induced cell apoptosis concomitant with upregulation of Bak, a Bcl-2 family member, and downregulation of signal transducers and activators of transcription 3 (STAT3) tyrosine phosphorylation. Tyrosine 207-215 signal transducer and activator of transcription 3 Homo sapiens 200-205 20196786-3 2009 SHP1 expression correlates with down-regulation of Janus kinase/signal transducers and activators of transcription (JAK2/STAT3/STAT5) signalling, which is mediated in part by tyrosine dephosphorylation events and modulation of the proteasome pathway. Tyrosine 175-183 signal transducer and activator of transcription 3 Homo sapiens 121-126 19712481-7 2009 Conversely, BITC was shown to inhibit the tyrosine phosphorylation of STAT3, but not the phosphorylation of ERK1/2, MAPK and p70S6 kinase. Tyrosine 42-50 signal transducer and activator of transcription 3 Homo sapiens 70-75 19470402-3 2009 STAT3 dimerization occurs through reciprocal interaction between the SH2 domain of one monomer and the phosphorylated tyrosine residue of a second one. Tyrosine 118-126 signal transducer and activator of transcription 3 Homo sapiens 0-5 19503092-6 2009 By contrast, CypA knockdown inhibited Stat3 IL-6-induced tyrosine phosphorylation and nuclear translocation. Tyrosine 57-65 signal transducer and activator of transcription 3 Homo sapiens 38-48 19723038-6 2009 Phosphorylation of STAT-3 at tyrosine 705 leads to its dimerization, nuclear translocation, DNA binding, and gene transcription. Tyrosine 29-37 signal transducer and activator of transcription 3 Homo sapiens 19-25 19556508-1 2009 Signal transducer and activator of transcription 3 (STAT3) is a latent cytoplasmic transcription factor responsive to cytokine signaling and tyrosine kinase oncoproteins by nuclear translocation when it is tyrosine-phosphorylated. Tyrosine 141-149 signal transducer and activator of transcription 3 Homo sapiens 0-50 19483716-8 2009 Moreover, STAT3 was dephosphorylated at tyrosine 705 in ST6Gal-I-knockdown (ST6Gal-I-KD) HCT116 cells. Tyrosine 40-48 signal transducer and activator of transcription 3 Homo sapiens 10-15 19427337-3 2009 Mutagenesis studies identified that phosphorylation of tyrosines 19 and 27 on caveolin-2 is required for the STAT3 activation. Tyrosine 55-64 signal transducer and activator of transcription 3 Homo sapiens 109-114 19427337-8 2009 Finally, insulin-induced transcriptional activation of STAT3 depended on phosphorylation of both 19 and 27 tyrosines. Tyrosine 107-116 signal transducer and activator of transcription 3 Homo sapiens 55-60 19556508-1 2009 Signal transducer and activator of transcription 3 (STAT3) is a latent cytoplasmic transcription factor responsive to cytokine signaling and tyrosine kinase oncoproteins by nuclear translocation when it is tyrosine-phosphorylated. Tyrosine 141-149 signal transducer and activator of transcription 3 Homo sapiens 52-57 19556508-3 2009 Moreover, STAT3 mutants that cannot be tyrosine-phosphorylated, that are retained in the cytoplasm, or that cannot bind DNA nonetheless supported Ras-mediated transformation. Tyrosine 39-47 signal transducer and activator of transcription 3 Homo sapiens 10-15 19159681-6 2009 Replacement of this tyrosine by phenylalanine (Y616F) disabled the auto-phosphorylation activity of Fer and abolished its ability to phosphorylate Stat3. Tyrosine 20-28 signal transducer and activator of transcription 3 Homo sapiens 147-152 19455144-9 2009 Most interestingly, while using primary tumour material, we observed that in contrast to non-small-cell lung cancer samples from primary tumour tissues, all analysed samples from SCLC were strongly positive for tyrosine-phosphorylated STAT3. Tyrosine 211-219 signal transducer and activator of transcription 3 Homo sapiens 235-240 19331815-7 2009 While tyrosine phosphorylation of STAT3 was unaffected by mTOR inhibition and knockdown, serine phosphorylation was diminished. Tyrosine 6-14 signal transducer and activator of transcription 3 Homo sapiens 34-39 19393627-0 2009 STAP-2 is phosphorylated at tyrosine-250 by Brk and modulates Brk-mediated STAT3 activation. Tyrosine 28-36 signal transducer and activator of transcription 3 Homo sapiens 75-80 19272817-2 2009 The purpose of this study was to evaluate the expression of tyrosine phosphorylated (active) Stat3 and survivin in various benign and malignant salivary gland tumors (SGTs). Tyrosine 60-68 signal transducer and activator of transcription 3 Homo sapiens 93-98 19272817-5 2009 RESULTS: Survivin was immunohistochemically detected in all studied benign and malignant SGTs; p-tyr Stat3 was also detected in the majority (91%) of SGTs. Tyrosine 97-100 signal transducer and activator of transcription 3 Homo sapiens 101-106 19232485-6 2009 Using Western blot analyses, we show that interferon-alpha stimulated STAT3 phosphorylation at tyrosine (Y705) residue in 17% of cases. Tyrosine 95-103 signal transducer and activator of transcription 3 Homo sapiens 70-75 19305428-5 2009 Importantly, a tyrosine to phenylalanine mutation of the major Src phosphorylation site on EGFR, Y845, reduced the constitutive phosphorylation of NSCLC-EGFR mutants, as well as that of STAT3, Akt, Erk and Src, and reduced the mutant EGFR-Src association as well as proliferation, migration and anchorage-independent growth. Tyrosine 15-23 signal transducer and activator of transcription 3 Homo sapiens 186-191 19351839-8 2009 Suppression of Ser(727) and Tyr(705) phosphorylation of STAT3 by KU-55933 reduced STAT3 transacting activity accompanied by elevation in DR5 expression. Tyrosine 28-31 signal transducer and activator of transcription 3 Homo sapiens 56-61 19351839-8 2009 Suppression of Ser(727) and Tyr(705) phosphorylation of STAT3 by KU-55933 reduced STAT3 transacting activity accompanied by elevation in DR5 expression. Tyrosine 28-31 signal transducer and activator of transcription 3 Homo sapiens 82-87 19347282-4 2009 The activation of STATs depends on phosphorylation on a single tyrosine residue (e.g., Tyr705 in STAT3 and Tyr694 in STAT5) in the C-terminal domain. Tyrosine 63-71 signal transducer and activator of transcription 3 Homo sapiens 18-23 18988672-5 2009 Under these conditions there was a marked reduction in IL-6-dependent STAT3 signaling, including decreased STAT3 tyrosine phosphorylation, loss in nuclear accumulation of STAT3, and impaired expression of target genes, such as fibrinogen-gamma. Tyrosine 113-121 signal transducer and activator of transcription 3 Homo sapiens 70-75 19182994-3 2009 Further studies show that both serine-727 and tyrosine-705 of Stat3 were phosphorylated while Ha-ras was overexpressed. Tyrosine 46-54 signal transducer and activator of transcription 3 Homo sapiens 62-67 19126399-5 2009 We characterized the transduction pathways induced by leptin in SH-OBRb cells and demonstrated that OBRb mediates tyrosine phosphorylation of STAT3, phosphorylation of ERK1/2, but not SAPK/JNK and p38 MAPK, in a dose and time dependent fashion. Tyrosine 114-122 signal transducer and activator of transcription 3 Homo sapiens 142-147 19137011-3 2009 Here we report significantly greater signal transducer and activator of transcription 3 (STAT3) and tyrosine phosphorylated STAT3 in human HCC tissues (P<0.0030 and P<0.0455, respectively) than in human normal liver. Tyrosine 100-108 signal transducer and activator of transcription 3 Homo sapiens 124-129 19137011-8 2009 Moreover, NSC 74859 inhibited tyrosine phosphorylation of STAT3 in HCC cells in vivo. Tyrosine 30-38 signal transducer and activator of transcription 3 Homo sapiens 58-63 19201396-4 2009 Western blotting analysis using monoclonal antibody against IL-6 or gp 130 verified that this IL-6-driven activity was through the activation of tyrosine phosphorylation (Tyr(705)) of STAT3 in cancer cells. Tyrosine 145-153 signal transducer and activator of transcription 3 Homo sapiens 184-189 19201396-4 2009 Western blotting analysis using monoclonal antibody against IL-6 or gp 130 verified that this IL-6-driven activity was through the activation of tyrosine phosphorylation (Tyr(705)) of STAT3 in cancer cells. Tyrosine 171-174 signal transducer and activator of transcription 3 Homo sapiens 184-189 18755494-8 2009 Thus, we evaluated the phosphorylation status of STAT 3 and STAT 5, and found highly hyperphosphorylated STAT 3 on tyrosine 705 but not STAT 5 in our 2 cases of ALK-positive DLBCL with clathrin-ALK fusion. Tyrosine 115-123 signal transducer and activator of transcription 3 Homo sapiens 105-111 19347282-4 2009 The activation of STATs depends on phosphorylation on a single tyrosine residue (e.g., Tyr705 in STAT3 and Tyr694 in STAT5) in the C-terminal domain. Tyrosine 63-71 signal transducer and activator of transcription 3 Homo sapiens 97-102 19136996-4 2008 Tyr(1138) --> STAT3 signaling suppresses feeding, but is not required for a number of other leptin actions. Tyrosine 0-3 signal transducer and activator of transcription 3 Homo sapiens 17-22 19274102-3 2009 Three compounds satisfied criteria of interaction analysis, competitively inhibited recombinant Stat3 binding to its immobilized pY-peptide ligand and inhibited IL-6-mediated tyrosine phosphorylation of Stat3. Tyrosine 175-183 signal transducer and activator of transcription 3 Homo sapiens 96-101 19274102-3 2009 Three compounds satisfied criteria of interaction analysis, competitively inhibited recombinant Stat3 binding to its immobilized pY-peptide ligand and inhibited IL-6-mediated tyrosine phosphorylation of Stat3. Tyrosine 175-183 signal transducer and activator of transcription 3 Homo sapiens 203-208 18755267-4 2008 Unlike Galpha(16)-mediated regulation of STAT3 in the same cells, cicaprost-induced STAT3 Tyr(705) phosphorylation was resistant to inhibition of Src and MEK while STAT3 Ser(727) phosphorylation distinctly required phosphatidylinositol-3 kinase. Tyrosine 90-93 signal transducer and activator of transcription 3 Homo sapiens 84-89 19068194-9 2008 CONCLUSION: LKB1 protein might inhibit malignant biological behavior of A549 cells partly by down-regulating the tyrosine-phosphorylated level of STAT3 protein. Tyrosine 113-121 signal transducer and activator of transcription 3 Homo sapiens 146-151 18981102-6 2008 We found that IL-21 induced tyrosine phosphorylation of STAT-3, STAT-1, and Erk1/2. Tyrosine 28-36 signal transducer and activator of transcription 3 Homo sapiens 56-62 18755267-4 2008 Unlike Galpha(16)-mediated regulation of STAT3 in the same cells, cicaprost-induced STAT3 Tyr(705) phosphorylation was resistant to inhibition of Src and MEK while STAT3 Ser(727) phosphorylation distinctly required phosphatidylinositol-3 kinase. Tyrosine 90-93 signal transducer and activator of transcription 3 Homo sapiens 84-89 18755267-3 2008 Stimulation of STAT3 Tyr(705) and Ser(727) phosphorylations by the IP-specific agonist, cicaprost, was sensitive to inhibition of protein kinase A, phospholipase Cbeta, protein kinase C, calmodulin-dependent protein kinase II and Janus kinase 2/3. Tyrosine 21-24 signal transducer and activator of transcription 3 Homo sapiens 15-20 18939995-2 2008 Activation of Stat3 is dependent on the phosphorylation at the tyrosine residue 705 by upstream kinases and subsequent nuclear translocation after dimerization. Tyrosine 63-71 signal transducer and activator of transcription 3 Homo sapiens 14-19 21479505-2 2008 Physiological STAT3 phosphorylation involves tyrosine (Y705) and serine (S727) activation. Tyrosine 45-53 signal transducer and activator of transcription 3 Homo sapiens 14-19 19001435-5 2008 The inhibition of phosphorylated STAT3 (Tyr(705)) occurs in a dose- and time-dependent manner. Tyrosine 40-43 signal transducer and activator of transcription 3 Homo sapiens 33-38 18829527-0 2008 Activation of signal transducer and activator of transcription 3 through a phosphomimetic serine 727 promotes prostate tumorigenesis independent of tyrosine 705 phosphorylation. Tyrosine 148-156 signal transducer and activator of transcription 3 Homo sapiens 14-64 18718905-5 2008 Leptin time- and dose-dependently stimulated tyrosine phosphorylation of STAT3 in both human and mouse JAK2-null cells. Tyrosine 45-53 signal transducer and activator of transcription 3 Homo sapiens 73-78 18796709-7 2008 This effect was paralleled by impaired tyrosine activation of STAT1 and STAT3. Tyrosine 39-47 signal transducer and activator of transcription 3 Homo sapiens 72-77 18593565-2 2008 CD95L-induced caspase activation leads to degradation of gp130, thereby suppressing IL-6-induced phosphorylation of STAT3 (Tyr(705)) and of tyrosine phosphatase SHP2 (Tyr(580)). Tyrosine 123-126 signal transducer and activator of transcription 3 Homo sapiens 116-121 18544087-9 2008 We further examined the effect of CTD on the IL-6 signaling pathway in myeloma cells, and found that CTD inhibited phosphorylation of STAT3 at tyrosine 705 residue as early as 1 h after treatment and down-regulated the expression of the antiapoptotic bcl-xL protein. Tyrosine 143-151 signal transducer and activator of transcription 3 Homo sapiens 134-139 18619412-6 2008 We have found that leptin stimulates Janus kinase (JAK)-signal transducers and activators of transcription (STAT) pathway by promoting JAK-2 and STAT-3 tyrosine phosphorylation. Tyrosine 152-160 signal transducer and activator of transcription 3 Homo sapiens 108-112 18619412-6 2008 We have found that leptin stimulates Janus kinase (JAK)-signal transducers and activators of transcription (STAT) pathway by promoting JAK-2 and STAT-3 tyrosine phosphorylation. Tyrosine 152-160 signal transducer and activator of transcription 3 Homo sapiens 145-151 18676737-8 2008 RESULTS AND CONCLUSIONS: STAT-3 activation, as assessed by tyrosine and serine phosphorylation, was elevated in GBM tissue compared with control tissue. Tyrosine 59-67 signal transducer and activator of transcription 3 Homo sapiens 25-31 18790749-6 2008 Specifically, in Hodgkin lymphoma L540 cells, treatment with AUH-6-96 resulted in reduced levels of tyrosine phosphorylated STAT3 and of the STAT3 downstream target gene SOCS3 in a dose- and time-dependent manner. Tyrosine 100-108 signal transducer and activator of transcription 3 Homo sapiens 124-129 18503751-1 2008 Signal transducer and activator of transcription 3 (STAT3), which mediates biological actions in many physiological processes, is activated by cytokines and growth factors via specific tyrosine-phosphorylation, dimerization, and nuclear translocation. Tyrosine 185-193 signal transducer and activator of transcription 3 Homo sapiens 0-50 18503751-1 2008 Signal transducer and activator of transcription 3 (STAT3), which mediates biological actions in many physiological processes, is activated by cytokines and growth factors via specific tyrosine-phosphorylation, dimerization, and nuclear translocation. Tyrosine 185-193 signal transducer and activator of transcription 3 Homo sapiens 52-57 18503751-6 2008 These results indicate that Y14 interacts with STAT3 and regulates the transcriptional activation of STAT3 by influencing the tyrosine-phosphorylation of STAT3. Tyrosine 126-134 signal transducer and activator of transcription 3 Homo sapiens 47-52 18503751-6 2008 These results indicate that Y14 interacts with STAT3 and regulates the transcriptional activation of STAT3 by influencing the tyrosine-phosphorylation of STAT3. Tyrosine 126-134 signal transducer and activator of transcription 3 Homo sapiens 101-106 18503751-6 2008 These results indicate that Y14 interacts with STAT3 and regulates the transcriptional activation of STAT3 by influencing the tyrosine-phosphorylation of STAT3. Tyrosine 126-134 signal transducer and activator of transcription 3 Homo sapiens 101-106 18483262-6 2008 Immunohistochemical analysis of STAT1 and phosphorylated tyrosine STAT3 (pSTAT3) in skin biopsies obtained from CTCL patients enrolled in the vorinostat phase IIb trial showed that nuclear accumulation of STAT1 and high levels of nuclear pSTAT3 in malignant T cells correlate with a lack of clinical response. Tyrosine 57-65 signal transducer and activator of transcription 3 Homo sapiens 66-71 18498667-11 2008 In addition, the activation of PLD by pervanadate triggered phosphorylation of tyrosine 705 residue on STAT-3, and its phosphorylation was dramatically higher in TPC-1 cells (from papillary carcinoma) that have an endogenous RET/PTC1 than in ARO cells (from anaplastic carcinoma) without alteration of total STAT-3 expression. Tyrosine 79-87 signal transducer and activator of transcription 3 Homo sapiens 103-109 18723478-8 2008 The Z3-mediated reduction in cell proliferation correlated with reduced Jak2 and STAT3 tyrosine phosphorylation levels as well as marked cell cycle arrest. Tyrosine 87-95 signal transducer and activator of transcription 3 Homo sapiens 81-86 18408167-2 2008 14,15-EET stimulated the tyrosine phosphorylation of STAT-3 and its translocation from the cytoplasm to the nucleus in human dermal microvascular endothelial cells (HDMVECs). Tyrosine 25-33 signal transducer and activator of transcription 3 Homo sapiens 53-59 18408167-4 2008 14,15-EET activated Src, as measured by its tyrosine phosphorylation and blockade of its activation by adenovirus-mediated expression of its dominant negative mutant, significantly attenuated 14,15-EET-induced STAT-3 phosphorylation in HDMVECs and the migration and tube formation of these cells and Matrigel plug angiogenesis. Tyrosine 44-52 signal transducer and activator of transcription 3 Homo sapiens 210-216 18353781-3 2008 In this study, we observed significantly increased STAT3 Tyr(705) phosphorylation, but not the STAT3 protein levels, in the I4 lines. Tyrosine 57-60 signal transducer and activator of transcription 3 Homo sapiens 51-56 18353781-10 2008 There was a strong correlation between Tyr(705) p-STAT3 and Twist level in the late stage tumor tissues. Tyrosine 39-42 signal transducer and activator of transcription 3 Homo sapiens 50-55 18287094-5 2008 Such effects are associated with impaired IFN-dependent phosphorylation of Stat1 on Tyr(701) and Stat3 on Tyr(705) and defective binding of Stat complexes to ISRE or GAS elements. Tyrosine 106-109 signal transducer and activator of transcription 3 Homo sapiens 97-102 18389480-9 2008 IL-12 and IL-23 tyrosine phosphorylated STAT3 and STAT1, respectively; IL-12, IL-23, and IL-27 tyrosine phosphorylated JAK2 and tyrosine kinase-2; and IL-27 tyrosine phosphorylated JAK1. Tyrosine 16-24 signal transducer and activator of transcription 3 Homo sapiens 40-45 18060032-1 2007 Persistently activated or tyrosine-phosphorylated STAT3 (pSTAT3) is found in 50% of lung adenocarcinomas. Tyrosine 26-34 signal transducer and activator of transcription 3 Homo sapiens 50-55 18308712-1 2008 Previous studies have demonstrated that neutrophils isolated from the blood of healthy donors do not respond to IL-10 in terms of either activation of signal transducer and activator of transcription-3 (STAT3) tyrosine phosphorylation or induction of suppressor of cytokine signalling (SOCS)-3 protein, unlike autologous mononuclear cells. Tyrosine 210-218 signal transducer and activator of transcription 3 Homo sapiens 203-208 18308712-4 2008 Consequently, septic neutrophils are promptly responsive to IL-10 in vitro, as revealed by a direct IL-10-mediated induction of STAT3 tyrosine phosphorylation and SOCS-3 gene transcription, mRNA and protein expression. Tyrosine 134-142 signal transducer and activator of transcription 3 Homo sapiens 128-133 18292512-6 2008 Moreover, IL-27 failed to activate STAT3, augment c-Myc and Pim-1 expression, and induce cell proliferation in pro-B BaF/3 transfectants expressing mutant gp130, in which the putative STAT3-binding four Tyr residues in the YXXQ motif of the cytoplasmic region was replaced by Phe. Tyrosine 203-206 signal transducer and activator of transcription 3 Homo sapiens 184-189 18070987-1 2008 B-non-Hodgkin lymphomas (B-NHLs) use a raft-associated signalosome made of the constitutively active Lyn kinase, the tyrosine phosphorylated Cbp/PAG adaptor, and tyrosine phosphorylated STAT3 transcription factor. Tyrosine 117-125 signal transducer and activator of transcription 3 Homo sapiens 186-191 18204781-8 2008 Furthermore, immunohistochemical analysis showed an increased nuclear STAT3 Tyr 705 staining in LMP1-positive cells and STAT3 Tyr 705 phosphorylation related to NPC stages III and IV. Tyrosine 126-129 signal transducer and activator of transcription 3 Homo sapiens 120-125 18583177-4 2008 Platelet-derived growth factor-BB induced human bone marrow mesenchymal stem cell proliferation via complete activation of the Janus-activated kinase-signal transducers and activators of transcription cascade, inducing signal transducer and activator of transcription 3 tyrosine and serine phosphorylation as well as Janus-activated kinase 2 tyrosine phosphorylation. Tyrosine 270-278 signal transducer and activator of transcription 3 Homo sapiens 219-269 17563747-3 2007 Transcriptional activity of Stat3 is controlled by Tyr-phosphorylation, followed by dimerization and nuclear translocation. Tyrosine 51-54 signal transducer and activator of transcription 3 Homo sapiens 28-33 18201931-6 2008 To study cytokine signaling, cell lysates were analyzed by Western blot for total or tyrosine-phosphorylated STAT3. Tyrosine 85-93 signal transducer and activator of transcription 3 Homo sapiens 109-114 18198175-5 2008 We show that expression of DeltaNp63alpha in Hep3B cells induces Stat3 phosphorylation on Tyr-705 and Ser-727. Tyrosine 90-93 signal transducer and activator of transcription 3 Homo sapiens 65-70 18237746-6 2008 This increase in SOCS-3 transcription is associated with a four- to fivefold higher level of tyrosine-phosphorylated STAT3 in the RACT-treated F9 Rex1(-/-) cells as compared to WT. Tyrosine 93-101 signal transducer and activator of transcription 3 Homo sapiens 117-122 18174171-6 2008 The tyrosine phosphorylation of STAT3 and subsequent gene induction induced by human or murine LIF are completely blocked by the respective inhibitor. Tyrosine 4-12 signal transducer and activator of transcription 3 Homo sapiens 32-37 18204781-3 2008 LMP1 stimulated STAT3 Tyr 705-dependent nuclear accumulation, as well as the phosphorylation of STAT3 at both Tyr 705 and Ser 727. Tyrosine 22-25 signal transducer and activator of transcription 3 Homo sapiens 16-21 18204781-3 2008 LMP1 stimulated STAT3 Tyr 705-dependent nuclear accumulation, as well as the phosphorylation of STAT3 at both Tyr 705 and Ser 727. Tyrosine 110-113 signal transducer and activator of transcription 3 Homo sapiens 96-101 18204781-4 2008 Treatment of cells with interleukin-6 neutralizing antibody inhibited the phosphorylation of STAT3 Tyr 705 and Ser 727. Tyrosine 99-102 signal transducer and activator of transcription 3 Homo sapiens 93-98 18204781-6 2008 The biological significance of JAK3-mediated activation of STAT3 Tyr 705 phosphorylation was further assessed by treating the cells with an inhibitor (WHI-P131) of JAK3. Tyrosine 65-68 signal transducer and activator of transcription 3 Homo sapiens 59-64 18204781-8 2008 Furthermore, immunohistochemical analysis showed an increased nuclear STAT3 Tyr 705 staining in LMP1-positive cells and STAT3 Tyr 705 phosphorylation related to NPC stages III and IV. Tyrosine 76-79 signal transducer and activator of transcription 3 Homo sapiens 70-75 18172037-3 2008 METHODS AND RESULTS: In cultured atrial myocytes and fibroblasts, Ang II induced tyrosine phosphorylation of STAT3 through a Rac1-dependent mechanism, which was inhibited by dominant-negative Rac1, losartan, and simvastatin. Tyrosine 81-89 signal transducer and activator of transcription 3 Homo sapiens 109-114 17956865-3 2007 IL-6 induces rapid nuclear translocation of Tyr-phosphorylated STAT3 that forms a nuclear complex with CDK9 in nondenaturing co-immunoprecipitation and confocal colocalization assays. Tyrosine 44-47 signal transducer and activator of transcription 3 Homo sapiens 63-68 17670892-3 2007 This targeting was rapid but transient, required phosphorylation and integrity of Tyr 705 in STAT3, and was blocked by nocodazole, geldanamycin, and indirubin E804 and by overexpression of wild-type caveolin-1. Tyrosine 82-85 signal transducer and activator of transcription 3 Homo sapiens 93-98 17948269-3 2007 Interestingly, the protein synthesis dependence of the latter phase coincides with a reduced ability of IL-10 to induce STAT3 tyrosine phosphorylation. Tyrosine 126-134 signal transducer and activator of transcription 3 Homo sapiens 120-125 17954917-6 2007 This activity depends on the tyrosine kinase Jak2, and two tyrosines within the intracellular domain of ephrinB1 are critical for the association with STAT3 and its activation. Tyrosine 59-68 signal transducer and activator of transcription 3 Homo sapiens 151-156 17967179-6 2007 Increased phosphorylation of Stat3 tyrosine 705 was also observed in this line. Tyrosine 35-43 signal transducer and activator of transcription 3 Homo sapiens 29-34 17967179-9 2007 Stat3 phosphorylation at tyrosine 705 was observed in IOSE-80PC cells, but was insufficient to allow for activation of the HGF promoter. Tyrosine 25-33 signal transducer and activator of transcription 3 Homo sapiens 0-5 17761947-6 2007 The results show that LKB1 inhibits rearranged in transformation (RET)/papillary thyroid carcinoma (PTC)-dependent activation of signal transducer and activator of transcription 3 (STAT3), which is mediated by phosphorylation of STAT3 tyrosine 705 by RET/PTC. Tyrosine 235-243 signal transducer and activator of transcription 3 Homo sapiens 129-179 17761947-6 2007 The results show that LKB1 inhibits rearranged in transformation (RET)/papillary thyroid carcinoma (PTC)-dependent activation of signal transducer and activator of transcription 3 (STAT3), which is mediated by phosphorylation of STAT3 tyrosine 705 by RET/PTC. Tyrosine 235-243 signal transducer and activator of transcription 3 Homo sapiens 181-186 17716862-2 2007 IL-6 initiates its action by binding to its cell surface receptor, followed by activation of Janus kinases and tyrosine phosphorylation of the signal transducer and transcription factor (STAT) 3. Tyrosine 111-119 signal transducer and activator of transcription 3 Homo sapiens 187-191 17716862-4 2007 Here we show that pretreatment of HepG2 hepatoma cells with methyl-beta-cyclodextrin (MbetaCD), which removes cholesterol and destroys lipid rafts, inhibited tyrosine phosphorylation of STAT3 in IL-6-activated, but not PV-activated cells. Tyrosine 158-166 signal transducer and activator of transcription 3 Homo sapiens 186-191 17716862-7 2007 The extent of IL-6-induced tyrosine phosphorylation of STAT3 was comparable in cells expressing low or high levels of caveolin. Tyrosine 27-35 signal transducer and activator of transcription 3 Homo sapiens 55-60 17716862-9 2007 The combined data suggest that STAT3 tyrosine phosphorylation occurs in preformed transducer complexes that can be activated in the absence of intact lipid rafts or caveolin. Tyrosine 37-45 signal transducer and activator of transcription 3 Homo sapiens 31-36 17586727-4 2007 All ERalpha-positive breast tumor cell lines displayed low basal activation of signal transducer and activator of transcription 3 (STAT3) until exposed to MSC, which induced chronic phosphorylation of STAT3 on tyrosine-705. Tyrosine 210-218 signal transducer and activator of transcription 3 Homo sapiens 79-129 17586727-4 2007 All ERalpha-positive breast tumor cell lines displayed low basal activation of signal transducer and activator of transcription 3 (STAT3) until exposed to MSC, which induced chronic phosphorylation of STAT3 on tyrosine-705. Tyrosine 210-218 signal transducer and activator of transcription 3 Homo sapiens 131-136 17586727-4 2007 All ERalpha-positive breast tumor cell lines displayed low basal activation of signal transducer and activator of transcription 3 (STAT3) until exposed to MSC, which induced chronic phosphorylation of STAT3 on tyrosine-705. Tyrosine 210-218 signal transducer and activator of transcription 3 Homo sapiens 201-206 17543500-5 2007 In contrast to the mechanism described for the rat gene our results indicate that IL-1beta suppresses the IL-6-induced activation of the human gamma-fibrinogen gene particularly by blocking the late phase STAT3-tyrosine phosphorylation NF-kappaB-dependently but independent from de novo protein synthesis. Tyrosine 211-219 signal transducer and activator of transcription 3 Homo sapiens 205-210 17917251-1 2007 Signal transducer and activator of transcription 3 (STAT3), which mediates biological actions in many physiological processes, is activated by cytokines and growth factors via specific tyrosine or serine phosphorylation, dimerization and nuclear translocation. Tyrosine 185-193 signal transducer and activator of transcription 3 Homo sapiens 0-50 17917251-1 2007 Signal transducer and activator of transcription 3 (STAT3), which mediates biological actions in many physiological processes, is activated by cytokines and growth factors via specific tyrosine or serine phosphorylation, dimerization and nuclear translocation. Tyrosine 185-193 signal transducer and activator of transcription 3 Homo sapiens 52-57 17646016-5 2007 Moreover, blocking the activity of Src family kinases reduced tyrosine phosphorylation of STAT1 and STAT3 and protected A431 cells from the EGF-induced growth inhibition. Tyrosine 62-70 signal transducer and activator of transcription 3 Homo sapiens 100-105 17602782-5 2007 RESULTS: Constitutively activated STAT3 through tyrosine phosphorylation was detected in all hepatoma cells. Tyrosine 48-56 signal transducer and activator of transcription 3 Homo sapiens 34-39 17726064-3 2007 We found that tyrosine phosphorylation of STAT3 by v-Src is independent of Janus kinases (Jaks), the canonical activators of STATs. Tyrosine 14-22 signal transducer and activator of transcription 3 Homo sapiens 42-47 17726064-8 2007 Inhibition of exportin-1-mediated nuclear export slowed down nucleocytoplasmic shuttling of v-Src-activated STAT3 resulting in reduced tyrosine phosphorylation, decreased induction of STAT3 target genes and increased apoptosis. Tyrosine 135-143 signal transducer and activator of transcription 3 Homo sapiens 108-113 17575268-4 2007 Alcohol alone induced and in combination with LPS augmented STAT3 phosphorylation at tyrosine 705 (tyr705) and serine 727 (ser727) residues and increased STAT3 binding to DNA. Tyrosine 85-93 signal transducer and activator of transcription 3 Homo sapiens 60-65 17636030-5 2007 Cooperative binding to DNA of tyrosine-phosphorylated STAT3 and both wild-type and R261A mutant c-Jun was observed. Tyrosine 30-38 signal transducer and activator of transcription 3 Homo sapiens 54-59 18432995-4 2007 Finally, a method to assess IL-21-induced tyrosine phosphorylation of Stat3 in splenic CD8(+) T cells using a flow cytometry-based analysis is described. Tyrosine 42-50 signal transducer and activator of transcription 3 Homo sapiens 70-75 17616678-2 2007 In these pathways, Janus-activated kinase (JAK)-induced transient tyrosine phosphorylation of STAT3 promotes gene expression in response to a number of cytokines, which is inhibited by feedback mechanisms. Tyrosine 66-74 signal transducer and activator of transcription 3 Homo sapiens 94-99 17385713-2 2007 Here we report that parthenolide reduced both basal and leukemia inhibitory factor (LIF)-induced STAT3 tyrosine 705 (Y705) phosphorylation in cardiomyocytes in a dose-dependent manner, but stimulated the MAP kinase signaling pathways. Tyrosine 103-111 signal transducer and activator of transcription 3 Homo sapiens 97-102 17543278-4 2007 LIGHT induces dose-dependent activation of Stat3 by phosphorylation at both the tyrosine 705 and serine 727 residues. Tyrosine 80-88 signal transducer and activator of transcription 3 Homo sapiens 43-48 17543278-7 2007 Overexpression of an active NIK induces Stat3 activation by phosphorylation at the both tyrosine 705 and serine 727 residues. Tyrosine 88-96 signal transducer and activator of transcription 3 Homo sapiens 40-45 17459060-1 2007 Signal transducers and activators of transcription-3 (STAT3), a central cytoplasmic transcription factor, is frequently overexpressed and constitutively activated by tyrosine during malignant transformation. Tyrosine 166-174 signal transducer and activator of transcription 3 Homo sapiens 0-52 17459060-1 2007 Signal transducers and activators of transcription-3 (STAT3), a central cytoplasmic transcription factor, is frequently overexpressed and constitutively activated by tyrosine during malignant transformation. Tyrosine 166-174 signal transducer and activator of transcription 3 Homo sapiens 54-59 17703129-6 2007 Moreover, we hypothesize that hyperactivation of the JAK kinases, particularly JAK2, mismatched STAT3 serine-tyrosine phosphorylation or heightened STAT3 transcriptional activity, and SOCS3 induction may ultimately prove detrimental. Tyrosine 109-117 signal transducer and activator of transcription 3 Homo sapiens 96-101 17566705-2 2007 Immunohistochemistry was used to detect the protein levels of phosphorylated-tyrosine Stat3 (p-tyr Stat3) and survivin in SCC tissues. Tyrosine 77-85 signal transducer and activator of transcription 3 Homo sapiens 86-91 17566705-4 2007 Immunohistochemical analysis revealed an overexpression of p-tyr Stat3 in T1 SCCs. Tyrosine 61-64 signal transducer and activator of transcription 3 Homo sapiens 65-70 17566705-2 2007 Immunohistochemistry was used to detect the protein levels of phosphorylated-tyrosine Stat3 (p-tyr Stat3) and survivin in SCC tissues. Tyrosine 77-85 signal transducer and activator of transcription 3 Homo sapiens 99-104 17566705-2 2007 Immunohistochemistry was used to detect the protein levels of phosphorylated-tyrosine Stat3 (p-tyr Stat3) and survivin in SCC tissues. Tyrosine 77-80 signal transducer and activator of transcription 3 Homo sapiens 99-104 17510282-1 2007 gp130-linked cytokines such as interleukin-6 (IL-6) stimulate the formation of tyrosine-phosphorylated signal transducer and activator of transcription 3 (P-STAT3), which activates many genes, including the STAT3 gene itself. Tyrosine 79-87 signal transducer and activator of transcription 3 Homo sapiens 207-212 16901975-1 2007 BACKGROUND: Constitutive activation of signal transducer and activator of transcription 3 at tyrosine residue 705 (p-STAT3 (tyr705)) has been associated with many types of human cancers. Tyrosine 93-101 signal transducer and activator of transcription 3 Homo sapiens 39-89 16901975-1 2007 BACKGROUND: Constitutive activation of signal transducer and activator of transcription 3 at tyrosine residue 705 (p-STAT3 (tyr705)) has been associated with many types of human cancers. Tyrosine 93-101 signal transducer and activator of transcription 3 Homo sapiens 117-122 17371842-8 2007 Their cooperative actions were likely through synergistic phosphorylation of signal transducer and activator of transcription 3 on tyrosine at position 705 and serine at position 727. Tyrosine 131-139 signal transducer and activator of transcription 3 Homo sapiens 77-127 17368812-3 2007 We found that Tyk2 and Stat3 are constitutively tyrosine phosphorylated in the leukemogenic cell line. Tyrosine 48-56 signal transducer and activator of transcription 3 Homo sapiens 23-28 17368569-0 2007 Leukemia inhibitory factor-induced phosphorylation of STAP-2 on tyrosine-250 is involved in its STAT3-enhancing activity. Tyrosine 64-72 signal transducer and activator of transcription 3 Homo sapiens 96-101 17439836-0 2007 Expression of anaplastic lymphoma kinase, tyrosine-phosphorylated STAT3, and associated factors in pediatric anaplastic large cell lymphoma: A report from the children"s oncology group. Tyrosine 42-50 signal transducer and activator of transcription 3 Homo sapiens 66-71 17483346-1 2007 15(S)-Hydroxyeicosatetraenoic acid [15(S)-HETE] activated signal transducer and activator of transcription 3 (STAT3) as measured by its tyrosine phosphorylation, translocation from the cytoplasm to the nucleus, DNA binding, and reporter gene activity in human dermal microvascular endothelial cells (HDMVEC). Tyrosine 136-144 signal transducer and activator of transcription 3 Homo sapiens 58-108 17483346-1 2007 15(S)-Hydroxyeicosatetraenoic acid [15(S)-HETE] activated signal transducer and activator of transcription 3 (STAT3) as measured by its tyrosine phosphorylation, translocation from the cytoplasm to the nucleus, DNA binding, and reporter gene activity in human dermal microvascular endothelial cells (HDMVEC). Tyrosine 136-144 signal transducer and activator of transcription 3 Homo sapiens 110-115 17368812-5 2007 Stat3 tyrosine phosphorylation can be induced in RH/K30 by treatment with IL-10. Tyrosine 6-14 signal transducer and activator of transcription 3 Homo sapiens 0-5 17043651-2 2007 STAT3 is activated by phosphorylation of a tyrosine residue, after which it dimerizes and translocates into the nucleus. Tyrosine 43-51 signal transducer and activator of transcription 3 Homo sapiens 0-5 17194701-5 2007 We were able to show an absolute requirement for both of the tyrosine residues found within the YXXQ-STAT3-docking site within the IL-10 receptor 1 and that no other signals appeared to be required. Tyrosine 61-69 signal transducer and activator of transcription 3 Homo sapiens 101-106 17204573-4 2007 Rapid and transient activation of phosphorylated (tyrosine 705) STAT3 was observed at 2 h postexercise. Tyrosine 50-58 signal transducer and activator of transcription 3 Homo sapiens 64-69 17432718-6 2007 This form is called tyrosine-705 phosphorylated Stat3, and is found in many malignant entities, including human gliomas. Tyrosine 20-28 signal transducer and activator of transcription 3 Homo sapiens 48-53 17173857-3 2007 Cigarette smoke extract (CSE) exposure increases STAT3 phosphorylation (Tyr 705) and DNA binding activity in HBECs. Tyrosine 72-75 signal transducer and activator of transcription 3 Homo sapiens 49-54 17078813-0 2007 Cr(VI)-stimulated STAT3 tyrosine phosphorylation and nuclear translocation in human airway epithelial cells requires Lck. Tyrosine 24-32 signal transducer and activator of transcription 3 Homo sapiens 18-23 17311011-5 2007 We examined tyrosine phosphorylation of Stat3 (activated form of Stat3) in multiple endometrial and cervical cancer tissues using tissue microarray slides as well as cancer cell lines to explore the possible activation of Stat3. Tyrosine 12-20 signal transducer and activator of transcription 3 Homo sapiens 40-45 17311011-5 2007 We examined tyrosine phosphorylation of Stat3 (activated form of Stat3) in multiple endometrial and cervical cancer tissues using tissue microarray slides as well as cancer cell lines to explore the possible activation of Stat3. Tyrosine 12-20 signal transducer and activator of transcription 3 Homo sapiens 65-70 17311011-5 2007 We examined tyrosine phosphorylation of Stat3 (activated form of Stat3) in multiple endometrial and cervical cancer tissues using tissue microarray slides as well as cancer cell lines to explore the possible activation of Stat3. Tyrosine 12-20 signal transducer and activator of transcription 3 Homo sapiens 65-70 17148439-7 2007 A single STAT3 recruitment site (Tyr-721) in the cytoplasmic domain of IL-31Ralpha exerts a dominant function in the entire receptor complex and is critical for gene induction, morphological changes, and growth inhibition. Tyrosine 33-36 signal transducer and activator of transcription 3 Homo sapiens 9-14 17360477-3 2007 Phosphorylation of a tyrosine at amino acid Y705 is essential for the function of STAT3, and PTPRT specifically dephosphorylated STAT3 at this position. Tyrosine 21-29 signal transducer and activator of transcription 3 Homo sapiens 82-87 17194445-7 2007 We showed that the IL-12 p35 silenced DCs decreased IL-12-induced T cell responses through blocking tyrosine phosphorylation of JAK2, TYK2, STAT3, and STAT4 proteins in T cells. Tyrosine 100-108 signal transducer and activator of transcription 3 Homo sapiens 140-145 17404037-1 2007 Interleukin-10 (IL-10) plays a major proliferative role in many tumors, and activates the transcription factor Stat3 by tyrosine phosphorylation. Tyrosine 120-128 signal transducer and activator of transcription 3 Homo sapiens 111-116 17367410-4 2007 BTK physically associated with and tyrosine phosphorylated STAT3; this association was promoted by pervanadate (PV)-induced oxidative stress. Tyrosine 35-43 signal transducer and activator of transcription 3 Homo sapiens 59-64 17404037-6 2007 Recombinant IL-10 (rIL-10) increased the tyrosine phosphorylation of Stat3, upregulated the levels of Fer, and increased the association of Fer with phosphorylated Stat3 (pYStat3). Tyrosine 41-49 signal transducer and activator of transcription 3 Homo sapiens 69-74 17531096-0 2007 Stat3 is tyrosine-phosphorylated through the interleukin-6/glycoprotein 130/Janus kinase pathway in breast cancer. Tyrosine 9-17 signal transducer and activator of transcription 3 Homo sapiens 0-5 17531096-1 2007 INTRODUCTION: Signal transducer and activator of transcription 3 (Stat3) is constitutively tyrosine-phosphorylated in approximately 50% of primary breast carcinomas. Tyrosine 91-99 signal transducer and activator of transcription 3 Homo sapiens 14-64 17531096-1 2007 INTRODUCTION: Signal transducer and activator of transcription 3 (Stat3) is constitutively tyrosine-phosphorylated in approximately 50% of primary breast carcinomas. Tyrosine 91-99 signal transducer and activator of transcription 3 Homo sapiens 66-71 17531096-3 2007 METHODS: We examined six breast cancer-derived cell lines expressing high or low levels of tyrosine-phosphorylated Stat3 (pStat3) as well as primary breast cancer specimens. Tyrosine 91-99 signal transducer and activator of transcription 3 Homo sapiens 115-120 17258468-5 2006 In our assays porins induce interleukin-6 (IL-6) release (110+/-2.6pg/ml) 24h after stimulation and STAT1/STAT3 tyrosine (Tyr701/Tyr705) and serine (Ser727) phosphorylation after 15min. Tyrosine 112-120 signal transducer and activator of transcription 3 Homo sapiens 106-111 17063503-3 2006 The objectives of the current study were to examine the effect of Stat3 activation on the phenotypic transformation of an immortalized, nontumorigenic ovarian epithelial cell line and to evaluate the expression of tyrosine-activated Stat3 (pStat3) in tissue microarrays from 303 ovarian carcinomas to determine its prognostic relevance and to correlate its expression with several upstream oncogenes of Stat3 and with the oncogenes involved in apoptosis and proliferation. Tyrosine 214-222 signal transducer and activator of transcription 3 Homo sapiens 233-238 17063503-3 2006 The objectives of the current study were to examine the effect of Stat3 activation on the phenotypic transformation of an immortalized, nontumorigenic ovarian epithelial cell line and to evaluate the expression of tyrosine-activated Stat3 (pStat3) in tissue microarrays from 303 ovarian carcinomas to determine its prognostic relevance and to correlate its expression with several upstream oncogenes of Stat3 and with the oncogenes involved in apoptosis and proliferation. Tyrosine 214-222 signal transducer and activator of transcription 3 Homo sapiens 233-238 17258468-8 2006 Inhibition of tyrosine/serine phosphorylation mediated by MAPKs of STAT1 and STAT3 decrease the IL-6 secretion following porin stimulation. Tyrosine 14-22 signal transducer and activator of transcription 3 Homo sapiens 77-82 17015686-3 2006 Only STAT3 was found constitutively tyrosine phosphorylated, but not other STATs. Tyrosine 36-44 signal transducer and activator of transcription 3 Homo sapiens 5-10 16956893-4 2006 The constitutive activity of oncogenic STAT3C was reported to depend on spontaneous dimerization directed by disulfide bonds in the absence of tyrosine phosphorylation. Tyrosine 143-151 signal transducer and activator of transcription 3 Homo sapiens 39-45 16956893-5 2006 In fact, tyrosine phosphorylation consequent upon cytokine or mitogen-induced signaling events remains obligatory for STAT3C activation. Tyrosine 9-17 signal transducer and activator of transcription 3 Homo sapiens 118-124 17121912-6 2006 Following irradiation, the level of phospho-Stat3 Tyr(705), but not phospho-Stat3 Ser(727), was reduced in HUVECs, whereas it remained unchanged in irradiated breast cancer cells. Tyrosine 50-53 signal transducer and activator of transcription 3 Homo sapiens 44-49 16567810-4 2006 In the present study, we show for the first time that AVP stimulation of vascular smooth muscle cells (VSMCs) induces STAT3 tyrosine and serine phosphorylation, followed by nuclear translocation of the phosphorylated STAT3. Tyrosine 124-132 signal transducer and activator of transcription 3 Homo sapiens 118-123 16762027-7 2006 Investigating signal transduction in lymphoblasts induced by these cytokines, we found that not only IL-2 but also IL-4 enhances signal transducer and activator of transcription 3 (STAT3) tyrosine phosphorylation by IL-12. Tyrosine 188-196 signal transducer and activator of transcription 3 Homo sapiens 129-179 16762027-7 2006 Investigating signal transduction in lymphoblasts induced by these cytokines, we found that not only IL-2 but also IL-4 enhances signal transducer and activator of transcription 3 (STAT3) tyrosine phosphorylation by IL-12. Tyrosine 188-196 signal transducer and activator of transcription 3 Homo sapiens 181-186 16762027-8 2006 Tyrosine phosphorylations of janus kinase 2 (JAK-2), tyrosine kinase 2 (TYK2), extracellular signal-regulated kinase (ERK) and STAT4, STAT5 and STAT6 were not potentiated by combinations of these cytokines, suggesting specificity for increased STAT3 phosphorylation. Tyrosine 0-8 signal transducer and activator of transcription 3 Homo sapiens 244-249 16568091-5 2006 Activation of STAT3 previously has been linked to oncogenesis, and results in this study demonstrate that STAT3 is tyrosine phosphorylated and transcriptionally activated in cells expressing endogenous Brk. Tyrosine 115-123 signal transducer and activator of transcription 3 Homo sapiens 14-19 16568091-5 2006 Activation of STAT3 previously has been linked to oncogenesis, and results in this study demonstrate that STAT3 is tyrosine phosphorylated and transcriptionally activated in cells expressing endogenous Brk. Tyrosine 115-123 signal transducer and activator of transcription 3 Homo sapiens 106-111 16800851-7 2006 Mutagenesis experiments show that cytokine-stimulated activation of M(2) promoter activity requires tyrosine residues on glycoprotein 130 (gp130) that are also required for both STAT1 and STAT3 activation. Tyrosine 100-108 signal transducer and activator of transcription 3 Homo sapiens 188-193 16751220-6 2006 Tyrosine phosphorylation of Stat3 by uPA was time dependent. Tyrosine 0-8 signal transducer and activator of transcription 3 Homo sapiens 28-33 16751220-9 2006 Single-chain uPA likewise induced tyrosine phosphorylation of Stat3 to a similar extent as intact tcuPA. Tyrosine 34-42 signal transducer and activator of transcription 3 Homo sapiens 62-67 16951338-0 2006 Monocyte 15-lipoxygenase expression is regulated by a novel cytosolic signaling complex with protein kinase C delta and tyrosine-phosphorylated Stat3. Tyrosine 120-128 signal transducer and activator of transcription 3 Homo sapiens 144-149 16951338-4 2006 We found a novel IL-13-dependent cytosolic signaling complex of PKCdelta and tyrosine-phosphorylated Stat3. Tyrosine 77-85 signal transducer and activator of transcription 3 Homo sapiens 101-106 16951338-6 2006 We therefore hypothesized that tyrosine phosphorylation was required for Stat3 interaction with PKCdelta and subsequent PKCdelta-dependent phosphorylation of Stat3 Ser727. Tyrosine 31-39 signal transducer and activator of transcription 3 Homo sapiens 73-78 16951338-6 2006 We therefore hypothesized that tyrosine phosphorylation was required for Stat3 interaction with PKCdelta and subsequent PKCdelta-dependent phosphorylation of Stat3 Ser727. Tyrosine 31-39 signal transducer and activator of transcription 3 Homo sapiens 158-163 16951338-9 2006 Furthermore, by transfecting monocytes with Stat3 containing mutations in Tyr705 or Ser727 or with wild-type Stat3, we demonstrated that both Stat3 tyrosine and serine phosphorylations are required for optimal binding of Stat3 with DNA and maximal expression of 15-lipoxygenase, an important regulator of inflammation and apoptosis. Tyrosine 148-156 signal transducer and activator of transcription 3 Homo sapiens 44-49 16951338-9 2006 Furthermore, by transfecting monocytes with Stat3 containing mutations in Tyr705 or Ser727 or with wild-type Stat3, we demonstrated that both Stat3 tyrosine and serine phosphorylations are required for optimal binding of Stat3 with DNA and maximal expression of 15-lipoxygenase, an important regulator of inflammation and apoptosis. Tyrosine 148-156 signal transducer and activator of transcription 3 Homo sapiens 109-114 16951338-9 2006 Furthermore, by transfecting monocytes with Stat3 containing mutations in Tyr705 or Ser727 or with wild-type Stat3, we demonstrated that both Stat3 tyrosine and serine phosphorylations are required for optimal binding of Stat3 with DNA and maximal expression of 15-lipoxygenase, an important regulator of inflammation and apoptosis. Tyrosine 148-156 signal transducer and activator of transcription 3 Homo sapiens 109-114 16951338-9 2006 Furthermore, by transfecting monocytes with Stat3 containing mutations in Tyr705 or Ser727 or with wild-type Stat3, we demonstrated that both Stat3 tyrosine and serine phosphorylations are required for optimal binding of Stat3 with DNA and maximal expression of 15-lipoxygenase, an important regulator of inflammation and apoptosis. Tyrosine 148-156 signal transducer and activator of transcription 3 Homo sapiens 109-114 16567810-7 2006 Furthermore, our results indicate that AVP-induced STAT3 tyrosine phosphorylation requires both JAK2 and c-Src tyrosine kinases. Tyrosine 57-65 signal transducer and activator of transcription 3 Homo sapiens 51-56 16567810-9 2006 We further suggest that AVP-induced STAT3 serine phosphorylation negatively modulates AVP-induced STAT3 tyrosine phosphorylation. Tyrosine 104-112 signal transducer and activator of transcription 3 Homo sapiens 36-41 16567810-9 2006 We further suggest that AVP-induced STAT3 serine phosphorylation negatively modulates AVP-induced STAT3 tyrosine phosphorylation. Tyrosine 104-112 signal transducer and activator of transcription 3 Homo sapiens 98-103 16651438-5 2006 Of interest, the rapid down-regulation in STAT3 was consistent with an accelerated ubiquitin-dependent degradation in the Tyr(705)-phosphorylated STAT3, but not the Ser(727)-phosphorylated one, another regulator of STAT3 activity. Tyrosine 122-125 signal transducer and activator of transcription 3 Homo sapiens 42-47 16611743-4 2006 Expression of catalytically inactive SHP-2 in DDR1-transfected cells restored the tyrosine phosphorylation of Stat3 and cell migration. Tyrosine 82-90 signal transducer and activator of transcription 3 Homo sapiens 110-115 16611743-6 2006 Mutation of tyrosine 703 or 796 of DDR1 abolished the ability of DDR1 to inhibit the tyrosine phosphorylation of Stat1 and Stat3 and restored collagen-induced cell migration and hepatocyte growth factor-induced branching tubulogenesis in collagen gel. Tyrosine 12-20 signal transducer and activator of transcription 3 Homo sapiens 123-128 16611743-6 2006 Mutation of tyrosine 703 or 796 of DDR1 abolished the ability of DDR1 to inhibit the tyrosine phosphorylation of Stat1 and Stat3 and restored collagen-induced cell migration and hepatocyte growth factor-induced branching tubulogenesis in collagen gel. Tyrosine 85-93 signal transducer and activator of transcription 3 Homo sapiens 123-128 16611743-7 2006 Together, these results demonstrate that SHP-2 is required for the DDR1-induced suppression of Stat1 and Stat3 tyrosine phosphorylation, cell migration, and branching tubulogenesis. Tyrosine 111-119 signal transducer and activator of transcription 3 Homo sapiens 105-110 16634641-8 2006 These data show that tyrosine phosphorylation is required for activation of STAT1C and STAT3C. Tyrosine 21-29 signal transducer and activator of transcription 3 Homo sapiens 87-93 16669628-2 2006 While phosphorylation of STAT3 at Tyr-705 has been demonstrated to be a prerequisite for STAT3 dimerization, nuclear translocation, and activation of gene transcription, the role of Ser-727 in regulation of STAT3 activity is controversial. Tyrosine 34-37 signal transducer and activator of transcription 3 Homo sapiens 25-30 16669628-2 2006 While phosphorylation of STAT3 at Tyr-705 has been demonstrated to be a prerequisite for STAT3 dimerization, nuclear translocation, and activation of gene transcription, the role of Ser-727 in regulation of STAT3 activity is controversial. Tyrosine 34-37 signal transducer and activator of transcription 3 Homo sapiens 89-94 16669628-2 2006 While phosphorylation of STAT3 at Tyr-705 has been demonstrated to be a prerequisite for STAT3 dimerization, nuclear translocation, and activation of gene transcription, the role of Ser-727 in regulation of STAT3 activity is controversial. Tyrosine 34-37 signal transducer and activator of transcription 3 Homo sapiens 89-94 16651438-4 2006 Consistent with the increased Fas/CD95 expression, a drastic decrease in the Tyr(705) phosphorylation of STAT3, a known negative regulator of Fas/CD95 transcription, was found within 20 minutes in the luteolin-treated cells, leading to down-regulation in the target gene products of STAT3, such as cyclin D1, survivin, Bcl-xL, and vascular endothelial growth factor. Tyrosine 77-80 signal transducer and activator of transcription 3 Homo sapiens 105-110 16651438-4 2006 Consistent with the increased Fas/CD95 expression, a drastic decrease in the Tyr(705) phosphorylation of STAT3, a known negative regulator of Fas/CD95 transcription, was found within 20 minutes in the luteolin-treated cells, leading to down-regulation in the target gene products of STAT3, such as cyclin D1, survivin, Bcl-xL, and vascular endothelial growth factor. Tyrosine 77-80 signal transducer and activator of transcription 3 Homo sapiens 283-288 16651438-5 2006 Of interest, the rapid down-regulation in STAT3 was consistent with an accelerated ubiquitin-dependent degradation in the Tyr(705)-phosphorylated STAT3, but not the Ser(727)-phosphorylated one, another regulator of STAT3 activity. Tyrosine 122-125 signal transducer and activator of transcription 3 Homo sapiens 146-151 16651438-5 2006 Of interest, the rapid down-regulation in STAT3 was consistent with an accelerated ubiquitin-dependent degradation in the Tyr(705)-phosphorylated STAT3, but not the Ser(727)-phosphorylated one, another regulator of STAT3 activity. Tyrosine 122-125 signal transducer and activator of transcription 3 Homo sapiens 146-151 16651438-9 2006 These data suggested that luteolin targeted STAT3 through dual pathways-the ubiquitin-dependent degradation in Tyr(705)-phosphorylated STAT3 and the gradual down-regulation in Ser(727)-phosphorylated STAT3 through inactivation of CDK5, thereby triggering apoptosis via up-regulation in Fas/CD95. Tyrosine 111-114 signal transducer and activator of transcription 3 Homo sapiens 44-49 16651438-9 2006 These data suggested that luteolin targeted STAT3 through dual pathways-the ubiquitin-dependent degradation in Tyr(705)-phosphorylated STAT3 and the gradual down-regulation in Ser(727)-phosphorylated STAT3 through inactivation of CDK5, thereby triggering apoptosis via up-regulation in Fas/CD95. Tyrosine 111-114 signal transducer and activator of transcription 3 Homo sapiens 135-140 16651438-9 2006 These data suggested that luteolin targeted STAT3 through dual pathways-the ubiquitin-dependent degradation in Tyr(705)-phosphorylated STAT3 and the gradual down-regulation in Ser(727)-phosphorylated STAT3 through inactivation of CDK5, thereby triggering apoptosis via up-regulation in Fas/CD95. Tyrosine 111-114 signal transducer and activator of transcription 3 Homo sapiens 135-140 16427044-6 2006 Strikingly, IFN-gamma-induced apoptosis and growth inhibition of M12 cells were associated with persistent suppression of the constitutive tyrosine-phosphorylated STAT3 (pY-STAT3). Tyrosine 139-147 signal transducer and activator of transcription 3 Homo sapiens 163-168 16427044-6 2006 Strikingly, IFN-gamma-induced apoptosis and growth inhibition of M12 cells were associated with persistent suppression of the constitutive tyrosine-phosphorylated STAT3 (pY-STAT3). Tyrosine 139-147 signal transducer and activator of transcription 3 Homo sapiens 173-178 16455651-5 2006 Surprisingly, the M704V, R706Q, and V743M mutations substantially reduced Fes autophosphorylation and STAT3 Tyr-705 phosphorylation compared with wild-type Fes, whereas S759F had little effect. Tyrosine 108-111 signal transducer and activator of transcription 3 Homo sapiens 102-107 16431927-6 2006 This reduction in Stat1 and Stat3 tyrosine phosphorylation by active PKR proceeds through TC-PTP, which is a substrate of the eukaryotic initiation factor-2alpha kinase both in vitro and in vivo. Tyrosine 34-42 signal transducer and activator of transcription 3 Homo sapiens 28-33 16431927-1 2006 Tyrosine phosphorylation of the transcription factors Stat1 and Stat3 is required for them to dimerize, translocate to the nucleus, and induce gene transcription. Tyrosine 0-8 signal transducer and activator of transcription 3 Homo sapiens 64-69 16603078-3 2006 Stat3 is a mediator of numerous growth factor and cytokine signaling pathways, all of which activate it through phosphorylation of tyrosine 705. Tyrosine 131-139 signal transducer and activator of transcription 3 Homo sapiens 0-5 16463269-3 2006 STAT3 activation was assessed by immunohistochemistry using a monoclonal antibody specific for tyrosine(705)-phosphorylated STAT3 (pSTAT3(tyr705)) and a tissue microarray containing 49 paraffin-embedded ESFT tumours with known EWS translocations. Tyrosine 95-103 signal transducer and activator of transcription 3 Homo sapiens 0-5 16463269-3 2006 STAT3 activation was assessed by immunohistochemistry using a monoclonal antibody specific for tyrosine(705)-phosphorylated STAT3 (pSTAT3(tyr705)) and a tissue microarray containing 49 paraffin-embedded ESFT tumours with known EWS translocations. Tyrosine 95-103 signal transducer and activator of transcription 3 Homo sapiens 124-129 16407171-3 2006 By 15-30 min after interleukin-6 (IL-6) treatment, up to two-thirds of cytoplasmic Tyr-phosphorylated STAT3 can be associated with the purified early endosome fraction (Rab-5-, EEA1-, transferrin receptor-, and clathrin-positive fraction). Tyrosine 83-86 signal transducer and activator of transcription 3 Homo sapiens 102-107 16247467-4 2006 Here we show that Galpha(12) stimulates the phosphorylation of STAT3 at both Tyrosine-705 and Serine-727 residues. Tyrosine 77-85 signal transducer and activator of transcription 3 Homo sapiens 63-68 16529541-3 2006 STAT3 and STAT5 acquire oncogenic potential through constitutive phosphorylation on tyrosine, and their activity has been shown to be required to sustain a transformed phenotype. Tyrosine 84-92 signal transducer and activator of transcription 3 Homo sapiens 0-5 16317687-4 2005 STAT3 was constitutively activated (i.e., tyrosine-phosphorylated) in cholangiocarcinoma cells but not in nonmalignant cholangiocytes. Tyrosine 42-50 signal transducer and activator of transcription 3 Homo sapiens 0-5 16048438-3 2005 Polyamine depletion by DFMO (alpha-difluoromethylornithine) caused phosphorylation of STAT3 at Tyr-705 and Ser-727. Tyrosine 95-98 signal transducer and activator of transcription 3 Homo sapiens 86-91 16048438-5 2005 Sustained phosphorylation of STAT3 at tyrosine residues was observed in polyamine-depleted cells after exposure to TNF-alpha. Tyrosine 38-46 signal transducer and activator of transcription 3 Homo sapiens 29-34 16048438-6 2005 Inhibition of STAT3 activation by AG490 or cell-membrane-permeant inhibitory peptide (PpYLKTK; where pY represents phospho-Tyr) increased the sensitivity of polyamine-depleted cells to apoptosis. Tyrosine 123-126 signal transducer and activator of transcription 3 Homo sapiens 14-19 16418226-4 2006 In this study, we assessed STAT3 activation downstream of this pathway and characterized the role of PKCdelta activation in STAT3 tyrosine and serine phosphorylation and keratinocyte proliferation. Tyrosine 130-138 signal transducer and activator of transcription 3 Homo sapiens 124-129 16418226-8 2006 In addition, overexpression of STAT3 tyrosine mutant eliminated insulin-induced PKCdelta activation and keratinocyte proliferation. Tyrosine 37-45 signal transducer and activator of transcription 3 Homo sapiens 31-36 16568834-3 2006 The activation of STAT proteins was estimated according to their tyrosine phosphorylation. Tyrosine 65-73 signal transducer and activator of transcription 3 Homo sapiens 18-22 15833272-12 2005 Serine phosphorylation of STAT-3 is mediated by ERK1/2, while tyrosine phosphorylation is mediated by Src. Tyrosine 62-70 signal transducer and activator of transcription 3 Homo sapiens 26-32 15998644-5 2005 STAT3 gets tyrosine-phosphorylated and translocated to the nucleus in response to IGF-1. Tyrosine 11-19 signal transducer and activator of transcription 3 Homo sapiens 0-5 15894558-5 2005 In this study, we show that IL-6 stimulates a transient increase of tyrosine phosphorylation of STAT3 in a dose-dependent fashion. Tyrosine 68-76 signal transducer and activator of transcription 3 Homo sapiens 96-101 16082218-6 2005 While the tyrosine phosphorylation of STAT3, which is indicative of its signal-dependent activation, is a common occurrence in tumors, and appears to play a crucial role in some malignancies, a variety of new data suggest that it can be dispensable under some circumstances and STAT3 can participate in transformation through novel and non-canonical mechanisms. Tyrosine 10-18 signal transducer and activator of transcription 3 Homo sapiens 38-43 16082218-6 2005 While the tyrosine phosphorylation of STAT3, which is indicative of its signal-dependent activation, is a common occurrence in tumors, and appears to play a crucial role in some malignancies, a variety of new data suggest that it can be dispensable under some circumstances and STAT3 can participate in transformation through novel and non-canonical mechanisms. Tyrosine 10-18 signal transducer and activator of transcription 3 Homo sapiens 278-283 15833272-9 2005 Inhibition of ERK1/2 by PD98059 prevented serine phosphorylation of STAT-3, whereas inhibition of Src by PP1 prevented STAT-3 tyrosine phosphorylation. Tyrosine 126-134 signal transducer and activator of transcription 3 Homo sapiens 119-125 15833272-11 2005 In conclusion, both serine and tyrosine phosphorylation of STAT-3 are involved in the signaling pathway mediating the effects of cyclic strain on vascular SMC. Tyrosine 31-39 signal transducer and activator of transcription 3 Homo sapiens 59-65 16140268-1 2005 Signal transducer and activator of transcription 3 (STAT3), which mediates biological actions in many physiological processes, is activated by cytokines and growth factors via specific tyrosine phosphorylation, dimerization, and nuclear translocation. Tyrosine 185-193 signal transducer and activator of transcription 3 Homo sapiens 0-50 16140268-1 2005 Signal transducer and activator of transcription 3 (STAT3), which mediates biological actions in many physiological processes, is activated by cytokines and growth factors via specific tyrosine phosphorylation, dimerization, and nuclear translocation. Tyrosine 185-193 signal transducer and activator of transcription 3 Homo sapiens 52-57 16140268-4 2005 Here, we re-examined the nature of the STAT3 RA mutant and found that it showed higher and more extensive tyrosine-phosphorylation as well as much higher STAT3 transcriptional activity in response to stimuli. Tyrosine 106-114 signal transducer and activator of transcription 3 Homo sapiens 39-44 15919823-0 2005 STAT3 nuclear import is independent of tyrosine phosphorylation and mediated by importin-alpha3. Tyrosine 39-47 signal transducer and activator of transcription 3 Homo sapiens 0-5 15919823-5 2005 Although tyrosine phosphorylation is required for STAT3 to bind to specific DNA target sites, nuclear import takes place constitutively and independently of tyrosine phosphorylation. Tyrosine 9-17 signal transducer and activator of transcription 3 Homo sapiens 50-55 15809078-2 2005 STAT3 is activated by cytokines and growth factors via the phosphorylation of a tyrosine residue, dimerization, and subsequent nuclear translocation. Tyrosine 80-88 signal transducer and activator of transcription 3 Homo sapiens 0-5 15809078-7 2005 Second, we showed that tyrosine-phosphorylated STAT3 associates, not only with importin alpha5/NPI-1 but also with other importin alphas, as a result of OSM stimulation, as evidenced by a solution binding assay. Tyrosine 23-31 signal transducer and activator of transcription 3 Homo sapiens 47-52 15749807-8 2005 Basal tyrosine phosphorylated (PY)-STAT3 was also significantly reduced by prolonged insulin treatment, and to a greater extent than total STAT3 protein levels. Tyrosine 6-14 signal transducer and activator of transcription 3 Homo sapiens 35-40 15649887-1 2005 Signal transducers and activators of transcription (Stat) belong to a family of latent cytoplasmic factors that can be activated by tyrosine phosphorylation by members of the Jak tyrosine kinase family in response to a variety of cytokines and growth factors. Tyrosine 132-140 signal transducer and activator of transcription 3 Homo sapiens 52-56 15764709-2 2005 STAT3 requires phosphorylation on Ser-727, in addition to tyrosine phosphorylation on Tyr-705, to be transcriptionally active. Tyrosine 58-66 signal transducer and activator of transcription 3 Homo sapiens 0-5 15764709-2 2005 STAT3 requires phosphorylation on Ser-727, in addition to tyrosine phosphorylation on Tyr-705, to be transcriptionally active. Tyrosine 86-89 signal transducer and activator of transcription 3 Homo sapiens 0-5 15805288-2 2005 Treatment of cells with R115777 inhibited the tyrosine phosphorylation of STAT3((Tyr705)), while increasing the serine phosphorylation of STAT3((Ser727)). Tyrosine 46-54 signal transducer and activator of transcription 3 Homo sapiens 74-79 15705894-1 2005 Signal transducer and activator of transcription 3 (STAT3) is phosphorylated on tyrosine residue 705 in response to growth factors or cytokines to form activated homodimers that drive gene expression. Tyrosine 80-88 signal transducer and activator of transcription 3 Homo sapiens 0-50 15615703-3 2005 Using human embryonic kidney HEK-293 cells stably expressing wild-type or mutated forms of CXCR4, we demonstrated that STAT3 phosphorylation requires the N-terminal part of the third intracellular loop (ICL3) and the tyrosine 157 present at the end of the second intracellular loop (ICL2) of CXCR4. Tyrosine 217-225 signal transducer and activator of transcription 3 Homo sapiens 119-124 15705894-1 2005 Signal transducer and activator of transcription 3 (STAT3) is phosphorylated on tyrosine residue 705 in response to growth factors or cytokines to form activated homodimers that drive gene expression. Tyrosine 80-88 signal transducer and activator of transcription 3 Homo sapiens 52-57 16158253-9 2005 DFMO also increased tyrosine phosphorylation of STAT-3 and expression of STAT-1 and JNK proteins. Tyrosine 20-28 signal transducer and activator of transcription 3 Homo sapiens 48-54 15650183-10 2005 Previous studies have shown that maximum STAT-3 transactivation requires Ser727 phosphorylation in addition to tyrosine phosphorylation. Tyrosine 111-119 signal transducer and activator of transcription 3 Homo sapiens 41-47 15665295-3 2005 Both intestinal trefoil peptide (TFF3) and VEGF, but not pS2 (TFF1), activate STAT3 signaling through Tyr(705) phosphorylation of both STAT3alpha and STAT3beta isoforms. Tyrosine 102-105 signal transducer and activator of transcription 3 Homo sapiens 78-83 15548519-8 2005 Thrombin activated STAT-3 as measured by its tyrosine phosphorylation and translocation from the cytoplasm to the nucleus. Tyrosine 45-53 signal transducer and activator of transcription 3 Homo sapiens 19-25 15548519-9 2005 Forced expression of a dominant negative mutant of STAT-3 reduced thrombin-induced STAT-3 tyrosine phosphorylation and its translocation from the cytoplasm to the nucleus. Tyrosine 90-98 signal transducer and activator of transcription 3 Homo sapiens 51-57 15548519-9 2005 Forced expression of a dominant negative mutant of STAT-3 reduced thrombin-induced STAT-3 tyrosine phosphorylation and its translocation from the cytoplasm to the nucleus. Tyrosine 90-98 signal transducer and activator of transcription 3 Homo sapiens 83-89 15653507-1 2005 Upon cytokine treatment, members of the signal transducers and activators of transcription (STAT) family of proteins are phosphorylated on tyrosine and serine sites within the carboxyl-terminal region in cells. Tyrosine 139-147 signal transducer and activator of transcription 3 Homo sapiens 92-96 15542633-7 2004 The lack of cPKC activity leads to a broad reduction of IFN-alpha-stimulated gene expression due to a significant impairment of STAT1 and STAT3 tyrosine phosphorylation. Tyrosine 144-152 signal transducer and activator of transcription 3 Homo sapiens 138-143 16706231-5 2005 In contrast, tyrphostin AG1478 abrogates tyrosine phosphorylation of PLCgamma1 and STAT3. Tyrosine 41-49 signal transducer and activator of transcription 3 Homo sapiens 83-88 15527783-7 2004 Tyr-705-phosphorylated STAT3 was localized mainly in the nucleus in mock infected cells, whereas STAT3 disappeared from the nucleus in virus-infected cells. Tyrosine 0-3 signal transducer and activator of transcription 3 Homo sapiens 23-28 15322111-3 2004 PDGF-BB stimulated tyrosine phosphorylation of Jak-2 and STAT-3 in a time-dependent manner in VSMCs. Tyrosine 19-27 signal transducer and activator of transcription 3 Homo sapiens 57-63 15527783-0 2004 Tyrosine dephosphorylation of STAT3 in SARS coronavirus-infected Vero E6 cells. Tyrosine 0-8 signal transducer and activator of transcription 3 Homo sapiens 30-35 15313471-5 2004 IL6, but not LIF or OSM inhibited proliferation, induced NE differentiation and tyrosine phosphorylation of STAT3 in LNCaP cells. Tyrosine 80-88 signal transducer and activator of transcription 3 Homo sapiens 108-113 15591791-5 2004 Furthermore, vanadate potentiated IL-6-signaling pathway by increasing the tyrosine phosphorylated levels of STAT3 (Tyr705), and Lyn. Tyrosine 75-83 signal transducer and activator of transcription 3 Homo sapiens 109-114 15322111-4 2004 In addition, AG490 and Jak-2KEpRK5, a selective pharmacological inhibitor and a dominant negative mutant, respectively, of Jak-2, attenuated PDGF-BB-induced STAT-3 tyrosine phosphorylation and its DNA binding and reporter gene activities. Tyrosine 164-172 signal transducer and activator of transcription 3 Homo sapiens 157-163 15363199-6 2004 Tyrosine-phosphorylated STAT3 level was measured by Western blot analysis. Tyrosine 0-8 signal transducer and activator of transcription 3 Homo sapiens 24-29 15286705-2 2004 Following tyrosine phosphorylation, STAT proteins translocate into the nucleus and activate specific target genes. Tyrosine 10-18 signal transducer and activator of transcription 3 Homo sapiens 36-40 15336564-2 2004 STATs were thought to exist as latent, cytoplasmic monomers and activation to require dimer formation was mediated exclusively by reciprocal phospho-tyrosine/SH2-domain interactions, but recent evidence of cytoplasmic STAT complexes, including dimers, and unphosphorylated STATs in the nucleus has challenged these notions. Tyrosine 149-157 signal transducer and activator of transcription 3 Homo sapiens 0-5 15325847-0 2004 Activation of formyl peptide receptor-like 1 by WKYMVm induces serine phosphorylation of STAT3, which inhibits its tyrosine phosphorylation and nuclear translocation induced by hydrogen peroxide. Tyrosine 115-123 signal transducer and activator of transcription 3 Homo sapiens 89-94 15325847-2 2004 The activation of STAT3 accompanies tyrosine phosphorylation and its translocation to the nucleus. Tyrosine 36-44 signal transducer and activator of transcription 3 Homo sapiens 18-23 15325847-4 2004 In this study, we observed that the stimulation of FPRL1 by Trp-Lys-Tyr-Met-Val-D-Met (WKYMVm) caused serine phosphorylation but not tyrosine phosphorylation of STAT3 in a pertussis toxin-sensitive manner. Tyrosine 68-71 signal transducer and activator of transcription 3 Homo sapiens 161-166 15363199-8 2004 RESULTS: Compared with control group, tyrosine-phosphorylated STAT3 level suppressed greatly in pEFSOCS3-transfected A549 cells (P< 0.01). Tyrosine 38-46 signal transducer and activator of transcription 3 Homo sapiens 62-67 15363199-11 2004 CONCLUSION: SOCS3 protein might inhibit proliferation of A549 cells by lowering the tyrosine-phosphorylated level of STAT3 protein. Tyrosine 84-92 signal transducer and activator of transcription 3 Homo sapiens 117-122 15194700-7 2004 We identify tyrosine residues 652 and 721 in the cytoplasmic region of the longest isoform of GPL (GPL(745)) as the major STAT5- and STAT3-activating sites, respectively. Tyrosine 12-20 signal transducer and activator of transcription 3 Homo sapiens 133-138 15242774-2 2004 Many cytokines including IFNs induce tyrosine phosphorylation of the STAT3 transcription factor, which regulates acute phase gene expression. Tyrosine 37-45 signal transducer and activator of transcription 3 Homo sapiens 69-74 15199060-6 2004 Flagellin-induced activation of STAT-3 was abolished via neutralizing antibodies to interleukin (IL)-6, but not interferon (IFN)beta nor IFNgamma; none of these neutralizing antibodies had any effect on flagellin-induced STAT-1 tyrosine phosphorylation. Tyrosine 228-236 signal transducer and activator of transcription 3 Homo sapiens 32-38 15242774-5 2004 Besides the interaction of tyrosine-phosphorylated STAT3 with ProTalpha in yeast cells, IFN induced the interaction of ProTalpha with STAT3 in mammalian cells, and this interaction was dependent on the tyrosine phosphorylation of STAT3. Tyrosine 27-35 signal transducer and activator of transcription 3 Homo sapiens 51-56 15242774-5 2004 Besides the interaction of tyrosine-phosphorylated STAT3 with ProTalpha in yeast cells, IFN induced the interaction of ProTalpha with STAT3 in mammalian cells, and this interaction was dependent on the tyrosine phosphorylation of STAT3. Tyrosine 202-210 signal transducer and activator of transcription 3 Homo sapiens 134-139 15242774-5 2004 Besides the interaction of tyrosine-phosphorylated STAT3 with ProTalpha in yeast cells, IFN induced the interaction of ProTalpha with STAT3 in mammalian cells, and this interaction was dependent on the tyrosine phosphorylation of STAT3. Tyrosine 202-210 signal transducer and activator of transcription 3 Homo sapiens 134-139 15184887-5 2004 Subcellular fractionation studies showed that a significant proportion of the expressed STAT3DN protein translocated to the nucleus, despite the fact that STAT3DN has a mutation at residue 705(tyrosine --> phenylalanine), a site that is believed to be crucial for STAT3 activation and nuclear translocation. Tyrosine 193-201 signal transducer and activator of transcription 3 Homo sapiens 88-93 15242752-7 2004 Moreover, different EDCs significantly decreased the tyrosine phosphorylation state of STAT3 and STAT5, showing a distinct effect with respect to E(2). Tyrosine 53-61 signal transducer and activator of transcription 3 Homo sapiens 87-92 15249664-3 2004 By immunohistochemical analysis, we observe a positive correlation between strong MMP-9 expression and tyrosine phosphorylated Stat3 in primary breast cancer specimens. Tyrosine 103-111 signal transducer and activator of transcription 3 Homo sapiens 127-132 15166220-2 2004 Their receptor is composed of two chains, interleukin-28R/likely interleukin or cytokine or receptor 2 (IL-28R/LICR2) and IL-10R beta, and mediates the tyrosine phosphorylation of STAT1, STAT2, STAT3, and STAT5. Tyrosine 152-160 signal transducer and activator of transcription 3 Homo sapiens 194-199 15077188-1 2004 STAT3 is frequently overexpressed and constitutively activated by tyrosine phosphorylation during malignant transformation. Tyrosine 66-74 signal transducer and activator of transcription 3 Homo sapiens 0-5 15262130-7 2004 When CD34(+) cells were stimulated by VEGF and SKOV3-supernatant, STAT3 appeared tyrosine-phosphorylation and nuclear translocation, but STAT5 was only phosphorylated, and not translocated. Tyrosine 81-89 signal transducer and activator of transcription 3 Homo sapiens 66-71 15077160-7 2004 Finally, activated form of STAT3, Tyr-705 phospho-stat3, was found in the nucleus of cancer cells in 11 of 40 (27.5%) human gastric cancer specimens. Tyrosine 34-37 signal transducer and activator of transcription 3 Homo sapiens 27-32 15077160-7 2004 Finally, activated form of STAT3, Tyr-705 phospho-stat3, was found in the nucleus of cancer cells in 11 of 40 (27.5%) human gastric cancer specimens. Tyrosine 34-37 signal transducer and activator of transcription 3 Homo sapiens 50-55 15116095-4 2004 Pretreatment with sodium arsenite strongly inhibited IL-6-inducible STAT3 tyrosine phosphorylation in HepG2 cells and did not affect its serine phosphorylation. Tyrosine 74-82 signal transducer and activator of transcription 3 Homo sapiens 68-73 15016839-4 2004 Human embryonic kidney 293 cell lines stably overexpressing OB-Rb show a dose-dependent leptin-induced signal transducer and activator of transcription 3 (STAT3) tyrosine phosphorylation and STAT3-responsive luciferase (STAT3-luc) activity. Tyrosine 162-170 signal transducer and activator of transcription 3 Homo sapiens 103-153 15016839-5 2004 We demonstrate that when SLR is incubated with free leptin, binding of leptin to OB-Rb is reduced, with corresponding decrease of leptin-induced STAT3 tyrosine phosphorylation and STAT3-luc activity. Tyrosine 151-159 signal transducer and activator of transcription 3 Homo sapiens 145-150 14966128-5 2004 Examination of a series of wild type and mutant Stat3 proteins demonstrated loss of binding to pYXXQ-containing peptides only in Stat3 mutated at Lys-591 or Arg-609, whose side chains interact with the Tyr(P) residue, and Stat3 mutated at Glu-638, whose amide hydrogen bonds with oxygen within the +3 Gln side chain when the peptide ligand assumes a beta-turn. Tyrosine 202-205 signal transducer and activator of transcription 3 Homo sapiens 48-53 15077188-7 2004 STAT3-Y705F-transfected HCT8/S11 cells display an increased tyrosine phosphorylation of the cell-cell adhesion regulator beta-catenin and its dissociation from the invasion suppressor E-cadherin at cell-cell contacts. Tyrosine 60-68 signal transducer and activator of transcription 3 Homo sapiens 0-5 14690454-5 2004 This STAT1-mediated inhibition does not require STAT1 tyrosine phosphorylation because overexpression of dominant-negative STAT1 with a mutation on tyrosine residue 701 also blocks IFN-alpha activation of STAT1, STAT2 and STAT3. Tyrosine 148-156 signal transducer and activator of transcription 3 Homo sapiens 222-227 15054106-3 2004 In the present study we show that MDCK cells also express the LIF-R and respond to stimulation with human LIF by activation of tyrosine phosphorylation of signal transducer and activator of transcription-3 (STAT3), both however in an unpolarised fashion. Tyrosine 127-135 signal transducer and activator of transcription 3 Homo sapiens 155-205 15054106-3 2004 In the present study we show that MDCK cells also express the LIF-R and respond to stimulation with human LIF by activation of tyrosine phosphorylation of signal transducer and activator of transcription-3 (STAT3), both however in an unpolarised fashion. Tyrosine 127-135 signal transducer and activator of transcription 3 Homo sapiens 207-212 14701810-2 2004 Upon stimulation cytoplasmic STAT3 is phosphorylated at tyrosine 705, translocates into the nucleus, and induces target genes. Tyrosine 56-64 signal transducer and activator of transcription 3 Homo sapiens 29-34 15010843-6 2004 Meanwhile, cisplatin also inhibited Stat3 tyrosine phosphorylation and down-regulated BcL-XL anti-apoptotic protein in the cancer cells tested. Tyrosine 42-50 signal transducer and activator of transcription 3 Homo sapiens 36-41 15115615-8 2004 Inhibition of ERK1/2 allowed the tyrosine of STAT3 to be phosphorylated in a dose-dependent manner to PD98059. Tyrosine 33-41 signal transducer and activator of transcription 3 Homo sapiens 45-50 14693161-9 2004 PDGF-BB, but not IL-4, induced STAT3 tyrosine and AKT serine phosphorylation. Tyrosine 37-45 signal transducer and activator of transcription 3 Homo sapiens 31-36 14696092-1 2004 Signal transducers and activators of transcription (STATs) are latent cytoplasmic transcription factors that are activated and translocated into the nucleus after phosphorylation at a conserved tyrosine residue. Tyrosine 194-202 signal transducer and activator of transcription 3 Homo sapiens 52-57 14625304-6 2004 After leptin, there was increased expression of tyrosine 1141-phosphorylated leptin receptor, which may contribute to STAT3 activation. Tyrosine 48-56 signal transducer and activator of transcription 3 Homo sapiens 118-123 15052767-5 2004 RESULTS: Tyrosine phosphorylation of STAT-3 and STAT-5 was undetectable in unstimulated CD34+ cells, but was evident at 15 min in response to VEGF stimulation. Tyrosine 9-17 signal transducer and activator of transcription 3 Homo sapiens 37-43 15052767-6 2004 VEGF resulted in a rapid and transient tyrosine phosphorylation of STAT-3 and STAT-5. Tyrosine 39-47 signal transducer and activator of transcription 3 Homo sapiens 67-73 14578356-7 2004 Antioxidant treatment blocks RSV-induced STAT binding to both the IRF-1 GAS and IRF-7 ISRE by inhibition of inducible STAT1 and STAT3 tyrosine phosphorylation, suggesting that RSV-induced ROS formation is required for STAT activation and IRF gene expression. Tyrosine 134-142 signal transducer and activator of transcription 3 Homo sapiens 128-133 12974672-1 2004 Signal transducer and activator of transcription 3 (Stat3) dimerization is commonly thought to be triggered by its tyrosine phosphorylation in response to interleukin-6 (IL-6) or other cytokines. Tyrosine 115-123 signal transducer and activator of transcription 3 Homo sapiens 0-50 14593105-2 2004 We have observed that a specific type of kappa B DNA sequence motif supports both NF-kappaB p65 homodimer binding and cooperativity with non-tyrosine-phosphorylated STAT3. Tyrosine 141-149 signal transducer and activator of transcription 3 Homo sapiens 165-170 12974672-1 2004 Signal transducer and activator of transcription 3 (Stat3) dimerization is commonly thought to be triggered by its tyrosine phosphorylation in response to interleukin-6 (IL-6) or other cytokines. Tyrosine 115-123 signal transducer and activator of transcription 3 Homo sapiens 52-57 12974672-7 2004 IL-6 induced a 2-fold increase of this basal FRET signal, indicating that tyrosine phosphorylation either increases the dimer/monomer ratio of Stat3 or induces a conformational change of the dimer yielding a higher FRET efficiency. Tyrosine 74-82 signal transducer and activator of transcription 3 Homo sapiens 143-148 14498832-4 2003 Since Ras-MAPK and STAT pathways are simultaneously stimulated by EGF, and Tyr-1086 and Tyr-1068 of EGFR are reported to be the binding sites for both Grb2 and Stat3, we investigated the possible regulatory role of Grb2 in STAT activation. Tyrosine 75-78 signal transducer and activator of transcription 3 Homo sapiens 160-165 14657276-6 2004 Studies in living cells have shown that the appearance of STAT3 nuclear bodies is transient, correlating with the timecourse of tyrosine-phosphorylation of STAT3. Tyrosine 128-136 signal transducer and activator of transcription 3 Homo sapiens 58-63 14657276-6 2004 Studies in living cells have shown that the appearance of STAT3 nuclear bodies is transient, correlating with the timecourse of tyrosine-phosphorylation of STAT3. Tyrosine 128-136 signal transducer and activator of transcription 3 Homo sapiens 156-161 14749507-3 2004 LRb initiates signaling via three major mechanisms: 1) Tyr(985) of LRb recruits SH2-containing tyrosine phosphatase (SHP-2); 2) Tyr(1138) of LRb recruits signal transducer and activator of transcription 3 (STAT3); and 3) tyrosine phosphorylation sites on the receptor-associated Jak2 likely recruit numerous undefined signaling proteins. Tyrosine 128-131 signal transducer and activator of transcription 3 Homo sapiens 154-204 14749507-3 2004 LRb initiates signaling via three major mechanisms: 1) Tyr(985) of LRb recruits SH2-containing tyrosine phosphatase (SHP-2); 2) Tyr(1138) of LRb recruits signal transducer and activator of transcription 3 (STAT3); and 3) tyrosine phosphorylation sites on the receptor-associated Jak2 likely recruit numerous undefined signaling proteins. Tyrosine 128-131 signal transducer and activator of transcription 3 Homo sapiens 206-211 14749507-4 2004 The Tyr(985) --> SHP-2 pathway is a major regulator of extracellular signal-regulated kinase (ERK) activation during leptin signaling in cultured cells, while the Tyr(1138) --> STAT3 pathway induces the feedback inhibitor, suppressor of cytokine signaling 3 (SOCS3), as well as important positive effectors of leptin action. Tyrosine 4-7 signal transducer and activator of transcription 3 Homo sapiens 183-188 14749507-4 2004 The Tyr(985) --> SHP-2 pathway is a major regulator of extracellular signal-regulated kinase (ERK) activation during leptin signaling in cultured cells, while the Tyr(1138) --> STAT3 pathway induces the feedback inhibitor, suppressor of cytokine signaling 3 (SOCS3), as well as important positive effectors of leptin action. Tyrosine 166-169 signal transducer and activator of transcription 3 Homo sapiens 183-188 14749507-6 2004 The Tyr(1138) --> STAT3 pathway mediates transcriptional regulation of the hypothalamic melanocortin pathway in vivo. Tyrosine 4-7 signal transducer and activator of transcription 3 Homo sapiens 21-26 14749507-8 2004 Interestingly, the Tyr(1138) --> STAT3 pathway does not strongly regulate neuropeptide Y (NPY) and thus is not required for the control of reproduction and growth. Tyrosine 19-22 signal transducer and activator of transcription 3 Homo sapiens 36-41 14498832-4 2003 Since Ras-MAPK and STAT pathways are simultaneously stimulated by EGF, and Tyr-1086 and Tyr-1068 of EGFR are reported to be the binding sites for both Grb2 and Stat3, we investigated the possible regulatory role of Grb2 in STAT activation. Tyrosine 88-91 signal transducer and activator of transcription 3 Homo sapiens 160-165 14498832-5 2003 In the present study, we report that transient expression of Grb2 specifically down-regulates EGF-stimulated tyrosine phosphorylation of Stat3, which leads to a repression of Stat3 transcriptional activity. Tyrosine 109-117 signal transducer and activator of transcription 3 Homo sapiens 137-142 14498832-5 2003 In the present study, we report that transient expression of Grb2 specifically down-regulates EGF-stimulated tyrosine phosphorylation of Stat3, which leads to a repression of Stat3 transcriptional activity. Tyrosine 109-117 signal transducer and activator of transcription 3 Homo sapiens 175-180 14498832-6 2003 In contrast, depletion of Grb2 by RNA interference substantially increases Stat3 tyrosine phosphorylation induced by EGF. Tyrosine 81-89 signal transducer and activator of transcription 3 Homo sapiens 75-80 14500551-10 2003 In conclusion, we show that: 1) TGF-beta receptor Type II is predominantly located on basolateral membrane and receptor stimulation activates Smad pathway; 2) TGF-beta1 down-regulates IL-6-induced tyrosine phoshorylation of STAT1 and STAT3 and ICAM-1 expression; and 3) Smad2 is required for the down-regulation of IL-6 signaling by TGF-beta. Tyrosine 197-205 signal transducer and activator of transcription 3 Homo sapiens 234-239 14750954-0 2003 Src-dependent phosphorylation of the EGF receptor Tyr-845 mediates Stat-p21waf1 pathway in A431 cells. Tyrosine 50-53 signal transducer and activator of transcription 3 Homo sapiens 67-71 14750954-4 2003 The phosphorylation of Tyr 845 can be inhibited by PP2, but not by AG1478, and is associated with Src activation and Stat 3/5 phosphorylation, but not with MAP (mitogen-activated protein) kinase phosphorylation. Tyrosine 23-26 signal transducer and activator of transcription 3 Homo sapiens 117-125 14750954-5 2003 Phosphorylation of Stat 3/5 in response to p52shc expression, EGF or H2O2 could also be inhibited by introduction into cells of phospho-Tyr 845-specific antibody or by expression of dominant-negative version of c-Src. Tyrosine 136-139 signal transducer and activator of transcription 3 Homo sapiens 19-27 14750954-7 2003 CONCLUSION: These results indicate that multiple signals for c-Src activation can promote Stat 3/5 phosphorylations through Src-dependent phosphorylation of EGFR on Tyr 845. Tyrosine 165-168 signal transducer and activator of transcription 3 Homo sapiens 90-98 14511121-6 2003 The enhancement was measured as an increase in tyrosine phosphorylated STAT3, in STAT3-DNA binding and in STAT-luciferase reporter gene activity. Tyrosine 47-55 signal transducer and activator of transcription 3 Homo sapiens 71-76 14522952-5 2003 The time course of enhanceosome occupation by GR and tyrosine-phosphorylated STAT3 shows that these transcription factors precede by approximately 5-10 min the arrival of RNA polymerase II (Pol II). Tyrosine 53-61 signal transducer and activator of transcription 3 Homo sapiens 77-82 14522952-8 2003 Compared with the total nuclear tyrosine-phosphorylated STAT3 capable of binding DNA, the chromatin-associated STAT3 resists dephosphorylation and appears to recycle to maintain the enhanceosome. Tyrosine 32-40 signal transducer and activator of transcription 3 Homo sapiens 56-61 14506160-0 2003 Differential expression and clinical significance of tyrosine-phosphorylated STAT3 in ALK+ and ALK- anaplastic large cell lymphoma. Tyrosine 53-61 signal transducer and activator of transcription 3 Homo sapiens 77-82 12842895-6 2003 EGF-induced phosphorylation of Stat3 at tyrosine 705 and Stat3-dependent transactivation were also impaired. Tyrosine 40-48 signal transducer and activator of transcription 3 Homo sapiens 31-36 14506160-5 2003 The STAT3 activation status in cell lines was determined using Western blots and an antibody that reacts specifically with the phosphorylated tyrosine 705 of STAT3, pSTAT3(tyr705). Tyrosine 142-150 signal transducer and activator of transcription 3 Homo sapiens 4-9 14506160-5 2003 The STAT3 activation status in cell lines was determined using Western blots and an antibody that reacts specifically with the phosphorylated tyrosine 705 of STAT3, pSTAT3(tyr705). Tyrosine 142-150 signal transducer and activator of transcription 3 Homo sapiens 158-163 12824281-4 2003 We found that VEGF rapidly induces STAT3 tyrosine phosphorylation and nuclear translocation. Tyrosine 41-49 signal transducer and activator of transcription 3 Homo sapiens 35-40 12913253-7 2003 In addition, IL-10 induced tyrosine (Tyr) phosphorylation of Erk, as well as serine (Ser) and Tyr phosphorylation of STAT-3. Tyrosine 94-97 signal transducer and activator of transcription 3 Homo sapiens 117-123 12913253-8 2003 Tyr phosphorylation of Erk and Ser phosphorylation of STAT-3 were inhibited by PD98059, while Tyr phosphorylation of STAT-3 was not inhibited by PD98059. Tyrosine 0-3 signal transducer and activator of transcription 3 Homo sapiens 54-60 12913253-8 2003 Tyr phosphorylation of Erk and Ser phosphorylation of STAT-3 were inhibited by PD98059, while Tyr phosphorylation of STAT-3 was not inhibited by PD98059. Tyrosine 94-97 signal transducer and activator of transcription 3 Homo sapiens 117-123 12746441-1 2003 Stat3 is activated by cytokines and growth factors via specific tyrosine phosphorylation, dimerization, and nuclear translocation. Tyrosine 64-72 signal transducer and activator of transcription 3 Homo sapiens 0-5 12890573-0 2003 Insulin attenuates leptin-induced STAT3 tyrosine-phosphorylation in a hepatoma cell line. Tyrosine 40-48 signal transducer and activator of transcription 3 Homo sapiens 34-39 12738762-6 2003 The up-regulation of Fer and its dissociation from Jak1 were accompanied by an augmented association of activated Fer with Stat3 and by a concomitant increase in the tyrosine phosphorylation of Stat3. Tyrosine 166-174 signal transducer and activator of transcription 3 Homo sapiens 194-199 12738762-7 2003 Dissociation of Fer and Jak1, as well as elevation in the level of Fer and in the tyrosine phosphorylation of Stat3, depended on the activity of phosphatidylinositol 3-kinase (PI3K) and was abolished by a PI3K inhibitor. Tyrosine 82-90 signal transducer and activator of transcription 3 Homo sapiens 110-115 12710894-4 2003 The ability of IL-6 to promote transcription of CRP is mediated, in large part, by activation of the transcription factor STAT3; this activation requires both a tyrosine phosphorylation (mediated by the IL-6 receptor complex) and a serine phosphorylation (Ser-727), the origin of which has been more obscure. Tyrosine 161-169 signal transducer and activator of transcription 3 Homo sapiens 122-127 12821943-5 2003 Tyrosine phosphorylation of STAT3/5 and of JAK2 also increased upon treatment of LU1205 cells with either PD or LY, suggesting that constitutive active MAPK and PI3K signals inhibit tyrosine phosphorylation of JAK/STATs. Tyrosine 0-8 signal transducer and activator of transcription 3 Homo sapiens 28-35 12821943-5 2003 Tyrosine phosphorylation of STAT3/5 and of JAK2 also increased upon treatment of LU1205 cells with either PD or LY, suggesting that constitutive active MAPK and PI3K signals inhibit tyrosine phosphorylation of JAK/STATs. Tyrosine 0-8 signal transducer and activator of transcription 3 Homo sapiens 214-219 12821943-5 2003 Tyrosine phosphorylation of STAT3/5 and of JAK2 also increased upon treatment of LU1205 cells with either PD or LY, suggesting that constitutive active MAPK and PI3K signals inhibit tyrosine phosphorylation of JAK/STATs. Tyrosine 182-190 signal transducer and activator of transcription 3 Homo sapiens 28-35 12821944-6 2003 To study the possible relevance of STAT3 activation by BCR-ABL in human CML, Western blot analyses performed on the CD34+ cells, purified from CML patients at different stages of their disease, also demonstrated increased levels of STAT3 proteins phosphorylated both on tyrosine and serine residues. Tyrosine 270-278 signal transducer and activator of transcription 3 Homo sapiens 232-237 12782602-5 2003 Furthermore, we found that conditioned medium harvested from HNSCC cells could induce STAT3 tyrosine phosphorylation in immortalized keratinocytes regardless of the status of EGFR signaling. Tyrosine 92-100 signal transducer and activator of transcription 3 Homo sapiens 86-91 12782602-6 2003 In contrast, blocking the cytokine gp130 coreceptor abolished STAT3 tyrosine phosphorylation in HNSCC cells and that induced by the conditioned medium. Tyrosine 68-76 signal transducer and activator of transcription 3 Homo sapiens 62-67 12595539-6 2003 IL-6 increased phosphorylation of STAT3 (at Tyr(705)) and ERK1/2 (at Tyr(204)) within 5 min that peaked at 15-30 min and returned to basal levels at 2 h. Phosphorylation of STAT3 was blocked by genistein, a protein tyrosine kinase inhibitor, and AG490, a JAK2 inhibitor, but not PD98059, an ERK1/2 kinase inhibitor. Tyrosine 44-47 signal transducer and activator of transcription 3 Homo sapiens 34-39 12894500-4 2003 In this study, we examined for the expression of the activated form of STAT3 [phospho-STAT3 (tyr 705)] in eleven cases of GIST by immunohistochemistry. Tyrosine 93-96 signal transducer and activator of transcription 3 Homo sapiens 71-76 12894500-4 2003 In this study, we examined for the expression of the activated form of STAT3 [phospho-STAT3 (tyr 705)] in eleven cases of GIST by immunohistochemistry. Tyrosine 93-96 signal transducer and activator of transcription 3 Homo sapiens 86-91 12894500-5 2003 All GISTs had strong nuclear and variable cytoplasmic expression of phospho-STAT3 (tyr 705). Tyrosine 83-86 signal transducer and activator of transcription 3 Homo sapiens 76-81 12782602-4 2003 Even in the EGFR-positive cell lines, STAT3 activation was not dependent on EGFR activation, as STAT3 tyrosine phosphorylation levels persisted after treatment with AG1478, a chemical inhibitor of EGFR activity. Tyrosine 102-110 signal transducer and activator of transcription 3 Homo sapiens 96-101 12763138-3 2003 Besides tyrosine 705 phosphorylation of STAT3, serine 727 phosphorylation has been described upon EPO stimulation. Tyrosine 8-16 signal transducer and activator of transcription 3 Homo sapiens 40-45 12684046-5 2003 However, we could determine a cytoplasmic activation of signal transducer and activator of transcription (STAT)-3 by phosphorylation of tyrosine 705 (Y705) within minutes only upon leptin stimulation in both keratinocyte cell lines. Tyrosine 136-144 signal transducer and activator of transcription 3 Homo sapiens 56-113 12670499-5 2003 Upon activation of the ORL(1) and fMLP receptors, phosphorylation of STAT3 at Tyr(705) was detected by immunoblot analysis. Tyrosine 78-81 signal transducer and activator of transcription 3 Homo sapiens 69-74 12667488-2 2003 The ubiquitous protein tyrosine phosphatase (PTP) called "T-cell protein tyrosine phosphatase" has been reported to mediate Tyr-dephosphorylation of both interferon-gamma (IFN-gamma)-induced PY-STAT1 and interkleukin-6 (IL-6)-induced PY-STAT3 in some cell lines. Tyrosine 124-127 signal transducer and activator of transcription 3 Homo sapiens 237-242 12634372-4 2003 EBV infection was associated with a significant increase in the tyrosine-phosphorylated forms of STAT3 and STAT5 in HeLa-Bx1 cells. Tyrosine 64-72 signal transducer and activator of transcription 3 Homo sapiens 97-102 12639901-4 2003 Incubation of AtT-20 cells with 10 ng/ml recombinant human NNT-1/BSF-3 rapidly induced tyrosine-phosphorylation of signal transducer and activator of transcription (STAT)3 and STAT1 at 5 and 10 min. Tyrosine 87-95 signal transducer and activator of transcription 3 Homo sapiens 115-171 12604627-4 2003 Leptin treatment at 24 or 48 h of culture resulted in dose-dependent 2- to 4-fold increases in tyrosine phosphorylation of STAT-3. Tyrosine 95-103 signal transducer and activator of transcription 3 Homo sapiens 123-129 12529382-6 2003 In addition, PDGF-BB activated STAT-3 as determined by its tyrosine phosphorylation, DNA-binding activity, and reporter gene expression, and these responses were suppressed by AG490, a selective inhibitor of Jak-2. Tyrosine 59-67 signal transducer and activator of transcription 3 Homo sapiens 31-37 12865943-4 2003 They also show reduced activation of Akt and reduced levels of tyrosine-phosphorylated STAT3, due to overexpression of the tumor suppressor, PTEN. Tyrosine 63-71 signal transducer and activator of transcription 3 Homo sapiens 87-92 12556491-6 2003 We further show that GPCR agonists stimulate tyrosine phosphorylation of STAT1 and STAT3 proteins in a Rac-dependent manner. Tyrosine 45-53 signal transducer and activator of transcription 3 Homo sapiens 83-88 12920809-2 2003 METHODS: Human colon cancer cell lines SW480 and HCT116 were transfected with Stat3 antisense oligonucleotide mediated by liposome, MTT assay was used to measure the proliferation, flow cytometry was applied to analyze the cell cycle, and the expressions of Stat3, phosphorylation-specific Stat3 (tyrosine 705), Cyclin D1, Cyclin E, CDK2, CDK4, CDK6, p21 and p27 were measured by western blot. Tyrosine 297-305 signal transducer and activator of transcription 3 Homo sapiens 78-83 12556491-7 2003 The tyrosine phosphorylation of STAT3 is biphasic; the first peak of phosphorylation is weak and correlates with rapid activation of Jaks by GPCRs, whereas the second peak is stronger and requires the synthesis of an autocrine factor. Tyrosine 4-12 signal transducer and activator of transcription 3 Homo sapiens 32-37 12474230-5 2003 The expression of the two phosphorylated (i.e. active) forms of STAT3, pSTAT3-tyr (phosphorylated at the tyrosine(705) residue) and pSTAT3-ser (phosphorylated at the serine(727) residue), was assessed in four MCL cell lines and 12 MCL tumours using western blots and/or immunofluorescence staining techniques. Tyrosine 105-113 signal transducer and activator of transcription 3 Homo sapiens 64-69 12361954-1 2002 The transcriptional regulation of Stat proteins is controlled through their C-terminal domains, which harbor both a tyrosine phosphorylation site, required for dimerization and subsequent nuclear translocation, and a serine phosphorylation site, required for maximum transcriptional activity. Tyrosine 116-124 signal transducer and activator of transcription 3 Homo sapiens 34-38 12193593-3 2002 5(S)-HETE also stimulated Jak-2, STAT-1, and STAT-3 tyrosine phosphorylation and STAT-3-DNA binding activity. Tyrosine 52-60 signal transducer and activator of transcription 3 Homo sapiens 45-51 12453907-6 2002 The HG-induced JAK2, STAT1, and STAT3 tyrosine phosphorylations in GMC were also abolished by AG-490. Tyrosine 38-46 signal transducer and activator of transcription 3 Homo sapiens 32-37 12235142-2 2002 Interleukin-6 (IL-6) initiates STAT3 signaling in plasma membrane rafts with the subsequent transit of Tyr-phosphorylated STAT3 (PY-STAT3) through the cytoplasmic compartment to the nucleus in association with accessory proteins. Tyrosine 103-106 signal transducer and activator of transcription 3 Homo sapiens 31-36 12235142-2 2002 Interleukin-6 (IL-6) initiates STAT3 signaling in plasma membrane rafts with the subsequent transit of Tyr-phosphorylated STAT3 (PY-STAT3) through the cytoplasmic compartment to the nucleus in association with accessory proteins. Tyrosine 103-106 signal transducer and activator of transcription 3 Homo sapiens 122-127 12235142-2 2002 Interleukin-6 (IL-6) initiates STAT3 signaling in plasma membrane rafts with the subsequent transit of Tyr-phosphorylated STAT3 (PY-STAT3) through the cytoplasmic compartment to the nucleus in association with accessory proteins. Tyrosine 103-106 signal transducer and activator of transcription 3 Homo sapiens 122-127 12543077-5 2002 However, the constitutive tyrosine phosphorylation of Stat3 as well as IL-2 induced Stat5 tyrosine phosphorylation and DNA binding were unaffected by the stably transfected wild-type SHP2 as well as the inactive SHP2. Tyrosine 26-34 signal transducer and activator of transcription 3 Homo sapiens 54-59 12193593-4 2002 Tyrphostin AG490, a specific inhibitor of Jak-2, significantly reduced tyrosine phosphorylation and DNA binding activity of STAT-3 and DNA synthesis induced by 5(S)-HETE. Tyrosine 71-79 signal transducer and activator of transcription 3 Homo sapiens 124-130 12359225-5 2002 Tyrosine-phosphorylated STAT3 directly interacted with a substrate-trapping mutant of TC-PTP. Tyrosine 0-8 signal transducer and activator of transcription 3 Homo sapiens 24-29 11940572-8 2002 Our results also suggest that tyrosine 1139 may be important for Src homology 2 domain association because a mutant lacking this tyrosine reduces the ability of the Src homology 2 domain to bind to ErbB-2 and significantly decreases its ability to activate Stat3 alpha. Tyrosine 30-38 signal transducer and activator of transcription 3 Homo sapiens 257-262 11940572-8 2002 Our results also suggest that tyrosine 1139 may be important for Src homology 2 domain association because a mutant lacking this tyrosine reduces the ability of the Src homology 2 domain to bind to ErbB-2 and significantly decreases its ability to activate Stat3 alpha. Tyrosine 129-137 signal transducer and activator of transcription 3 Homo sapiens 257-262 12070153-5 2002 Phosphopeptide competition analysis revealed multiple tyrosine residues within the C-tail that can act as the docking sites for both Stat1 and Stat3. Tyrosine 54-62 signal transducer and activator of transcription 3 Homo sapiens 143-148 12351686-3 2002 In endothelial cells, Stat5 and Stat3 are rapidly phosphorylated on both tyrosine and serine residues in response to 17beta-estradiol, and nuclear translocation is subsequently induced. Tyrosine 73-81 signal transducer and activator of transcription 3 Homo sapiens 32-37 12105218-0 2002 STAT3 activation by type I interferons is dependent on specific tyrosines located in the cytoplasmic domain of interferon receptor chain 2c. Tyrosine 64-73 signal transducer and activator of transcription 3 Homo sapiens 0-5 12105218-8 2002 In this report, we define the role of the type I IFN receptor in STAT3 activation and identify for the first time tyrosine residues present in the cytoplasmic domain of IFNAR2c that are critical for STAT3 activation. Tyrosine 114-122 signal transducer and activator of transcription 3 Homo sapiens 199-204 12105218-9 2002 The regulation of STAT3 activation by IFNs was measured in a human lung fibrosarcoma cell line lacking IFNAR2c but stably expressing various IFNAR2c tyrosine mutants. Tyrosine 149-157 signal transducer and activator of transcription 3 Homo sapiens 18-23 12105218-10 2002 We show here that in addition to IFN-dependent tyrosine phosphorylation of STAT3, activation using a STAT3-dependent electrophoretic mobility shift assay and a STAT3-specific reporter can also be demonstrated. Tyrosine 47-55 signal transducer and activator of transcription 3 Homo sapiens 75-80 12105218-11 2002 Furthermore, we demonstrate that type I IFN-dependent activation of STAT3 proceeds through a novel mechanism that is dependent on two tyrosines, Tyr(337) and Tyr(512), present in IFNAR2c and contained within a conserved six-amino acid residue motif, GxGYxM. Tyrosine 134-143 signal transducer and activator of transcription 3 Homo sapiens 68-73 12105218-11 2002 Furthermore, we demonstrate that type I IFN-dependent activation of STAT3 proceeds through a novel mechanism that is dependent on two tyrosines, Tyr(337) and Tyr(512), present in IFNAR2c and contained within a conserved six-amino acid residue motif, GxGYxM. Tyrosine 145-148 signal transducer and activator of transcription 3 Homo sapiens 68-73 12105218-11 2002 Furthermore, we demonstrate that type I IFN-dependent activation of STAT3 proceeds through a novel mechanism that is dependent on two tyrosines, Tyr(337) and Tyr(512), present in IFNAR2c and contained within a conserved six-amino acid residue motif, GxGYxM. Tyrosine 158-161 signal transducer and activator of transcription 3 Homo sapiens 68-73 12360405-7 2002 STAT3 tyrosine phosphorylation was inhibited in a dose dependent manner by the MEK inhibitor U0126, but not by inhibitors of PI-3K or Src kinases. Tyrosine 6-14 signal transducer and activator of transcription 3 Homo sapiens 0-5 12360405-9 2002 These results indicate that the MAPK pathway plays a critical role in IL-6 mediated tyrosine phosphorylation of STAT3 and suggests that Jak kinases may not be required in this cascade. Tyrosine 84-92 signal transducer and activator of transcription 3 Homo sapiens 112-117 12356680-3 2002 Furthermore, we report that the ability of IL-10 to up-regulate IL-1ra mRNA expression in IL-4-treated neutrophils requires 5-6 h and it is preceded by the acquisition of the capacity to activate Stat3 tyrosine phosphorylation. Tyrosine 202-210 signal transducer and activator of transcription 3 Homo sapiens 196-201 12235247-7 2002 AGL 2592 induces a dose-dependent and time-dependent inhibition of tyrosine phosphorylation of numerous proteins, including Stat3, and an increase of Bcl-2 phosphorylation, both biochemical hallmarks of growth inhibition and apoptosis. Tyrosine 67-75 signal transducer and activator of transcription 3 Homo sapiens 124-129 12208879-6 2002 We now report that HCV core protein directly interacts with and activates STAT3 through phosphorylation of the critical tyrosine residue. Tyrosine 120-128 signal transducer and activator of transcription 3 Homo sapiens 74-79 12152985-7 2002 Among the biochemical and molecular events mediated by these cytokines are the down-regulation of p27kip1 and the independent phosphorylation of STAT3 on tyrosine and serine residues. Tyrosine 154-162 signal transducer and activator of transcription 3 Homo sapiens 145-150 12075083-5 2002 Recent investigation of the possible functional roles of PTEN during papilloma development has now indicated that the level of tyrosine(705)-phosphorylated signal transducer and activator of transcription 3 [PTyr(705)STAT3] could be inversely correlated to that of PTEN as well. Tyrosine 127-135 signal transducer and activator of transcription 3 Homo sapiens 156-206 12075083-5 2002 Recent investigation of the possible functional roles of PTEN during papilloma development has now indicated that the level of tyrosine(705)-phosphorylated signal transducer and activator of transcription 3 [PTyr(705)STAT3] could be inversely correlated to that of PTEN as well. Tyrosine 127-135 signal transducer and activator of transcription 3 Homo sapiens 217-222 12075083-8 2002 Transfection of PTEN cDNA into HeLa cells attenuated STAT3 phosphorylation at Tyr(705) in a dose-dependent manner. Tyrosine 78-81 signal transducer and activator of transcription 3 Homo sapiens 53-58 11875080-7 2002 A proximal region of the EPO-R lacking cytoplasmic tyrosines couples to STAT1 and STAT3 phosphorylation as well as ERK and p38(HOG) activation, but not JNK/SAPK. Tyrosine 51-60 signal transducer and activator of transcription 3 Homo sapiens 82-87 11882386-3 2002 In the human cell line (SH-SY5Y), STAT1 and STAT3 activation by CNTF-like cytokines showed tyrosine phosphorylation peaking at 0.5 h and inactivating within 2 h. Tyrosine phosphorylation of the receptor-associated tyrosine kinases Jak1 and Jak2 showed a similar time course of activation and inactivation in response to CNTF. Tyrosine 91-99 signal transducer and activator of transcription 3 Homo sapiens 44-49 12082540-3 2002 Our results demonstrate that expression of wt p53 but not mutant p53 significantly reduced tyrosine phosphorylation of Stat3 and inhibited Stat3 DNA binding activity in both DU145 and Tsu prostate cancer cell lines that express constitutively active Stat3. Tyrosine 91-99 signal transducer and activator of transcription 3 Homo sapiens 119-124 11882386-3 2002 In the human cell line (SH-SY5Y), STAT1 and STAT3 activation by CNTF-like cytokines showed tyrosine phosphorylation peaking at 0.5 h and inactivating within 2 h. Tyrosine phosphorylation of the receptor-associated tyrosine kinases Jak1 and Jak2 showed a similar time course of activation and inactivation in response to CNTF. Tyrosine 162-170 signal transducer and activator of transcription 3 Homo sapiens 44-49 11960349-0 2002 Downregulation of IL-6-induced STAT3 tyrosine phosphorylation by TGF-beta1 is mediated by caspase-dependent and -independent processes. Tyrosine 37-45 signal transducer and activator of transcription 3 Homo sapiens 31-36 11812786-8 2002 Transfection of STAT3-deficient keratinocytes with an adenoviral vector encoding the wild-type Stat3 gene reversed not only impaired migration but also the increased tyrosine phosphorylation of p130(cas). Tyrosine 166-174 signal transducer and activator of transcription 3 Homo sapiens 16-21 11812786-8 2002 Transfection of STAT3-deficient keratinocytes with an adenoviral vector encoding the wild-type Stat3 gene reversed not only impaired migration but also the increased tyrosine phosphorylation of p130(cas). Tyrosine 166-174 signal transducer and activator of transcription 3 Homo sapiens 95-100 11812786-9 2002 These results strongly suggest that STAT3 in keratinocytes plays a critical role in turnover of tyrosine phosphorylation of p130(cas), modulating cell adhesiveness to the substratum leading to growth factor-dependent cell migration. Tyrosine 96-104 signal transducer and activator of transcription 3 Homo sapiens 36-41 11948098-3 2002 STATs are activated by tyrosine phosphorylation, which is normally a transient and tightly regulated process. Tyrosine 23-31 signal transducer and activator of transcription 3 Homo sapiens 0-5 11960349-2 2002 A reduction of STAT3 tyrosine phosphorylation after TGF-beta1 pretreatment was observed in four out of 40 AML cases (10%), although all of the AML cases responded to TGF-beta1 by means of SMAD3 translocation. Tyrosine 21-29 signal transducer and activator of transcription 3 Homo sapiens 15-20 11960349-3 2002 The reduced IL-6-mediated STAT3 tyrosine phosphorylation after pre-treatment with TGF-beta1 was associated with apoptosis and coincided with the degradation of certain cellular proteins, including JAK1 and -2 and Tyk2, without affecting the ERK expression and phosphorylation. Tyrosine 32-40 signal transducer and activator of transcription 3 Homo sapiens 26-31 11960349-4 2002 Furthermore, treatment of AML blasts with the cytostatic agent VP16, as an alternative way to induce apoptosis, resulted in a similar degree of degradation of JAK kinases and concomitant reduction of IL-6-mediated STAT3 tyrosine phosphorylation. Tyrosine 220-228 signal transducer and activator of transcription 3 Homo sapiens 214-219 11960349-5 2002 Although degradation of JAK kinases could be rescued by incubating the cells with the pan-caspase inhibitor Z-VAD-fmk, the attenuating effect of TGF-beta1 treatment on the STAT3 tyrosine phosphorylation was still partly present. Tyrosine 178-186 signal transducer and activator of transcription 3 Homo sapiens 172-177 11849447-10 2002 IL-6-induced STAT3 tyrosine phosphorylation and DNA binding activity was inhibited by treatment with Jak specific inhibitor AG 490; however, it was not affected by the MEK1 inhibitor PD 98059. Tyrosine 19-27 signal transducer and activator of transcription 3 Homo sapiens 13-18 11773079-2 2002 Following tyrosine phosphorylation, STAT3 proteins dimerize, translocate to the nucleus, and activate specific target genes. Tyrosine 10-18 signal transducer and activator of transcription 3 Homo sapiens 36-41 12061840-7 2002 Recombinant human Oncostatin M (rhOSM) can induce the transient tyrosine 705 phosphorylation of STAT3 in serum-starved LIFR beta/OSMR beta expressing ovarian carcinoma cell lines, but does not alter cell growth and effects only a modest increase in the apoptotic rate in these cultured cells. Tyrosine 64-72 signal transducer and activator of transcription 3 Homo sapiens 96-101 11751994-5 2002 STAT3 displayed tyrosine phosphorylation and DNA binding in all (four of four) ALK+ TCL cell lines tested. Tyrosine 16-24 signal transducer and activator of transcription 3 Homo sapiens 0-5 11796503-7 2002 GH stimulated tyrosine phosphorylation of the GH receptor, signal transducer and activator of transcription-3 (Stat3), and Stat5. Tyrosine 14-22 signal transducer and activator of transcription 3 Homo sapiens 111-116 11740864-0 2002 Cell type-specific and tyrosine phosphorylation-independent nuclear presence of STAT1 and STAT3. Tyrosine 23-31 signal transducer and activator of transcription 3 Homo sapiens 90-95 11751994-11 2002 Treatment with the PP2A inhibitor calyculin A abrogated tyrosine phosphorylation of STAT3. Tyrosine 56-64 signal transducer and activator of transcription 3 Homo sapiens 84-89 11751994-6 2002 The activation of STAT3 was selective because none of the other known STATs was consistently tyrosine phosphorylated in these cell lines. Tyrosine 93-101 signal transducer and activator of transcription 3 Homo sapiens 18-23 11751994-7 2002 In addition, malignant cells in tissue sections from all (10 of 10) ALK+ TCL patients expressed tyrosine-phosphorylated STAT3. Tyrosine 96-104 signal transducer and activator of transcription 3 Homo sapiens 120-125 11728804-3 2001 Oxidized LDL (OxLDL) also initiated STAT1 and STAT3 Tyr-phosphorylation and translocation to the nucleus, with a more marked effect for the extensively modified CuLDL. Tyrosine 52-55 signal transducer and activator of transcription 3 Homo sapiens 46-51 11777539-2 2002 Here we establish that IL-10 induces phosphorylation of Stat3 on Tyr(705) and serves as a survival factor for N13 microglial cells. Tyrosine 65-68 signal transducer and activator of transcription 3 Homo sapiens 56-61 11546821-4 2001 We found that Gas6 tyrosine phosphorylates STAT3 (signal transducers and activators of transcription) with concomitant translocation to the nucleus and induces STAT3-dependent transcriptional activation in cultured mesangial cells. Tyrosine 19-27 signal transducer and activator of transcription 3 Homo sapiens 43-48 11728804-6 2001 These results demonstrate that OxLDL induces the activation by Tyr-phosphorylation of JAK2, STAT1, and STAT3 by generation of an intracellular oxidative stress by means of its lipid peroxidation products, and thus include JAK2 within the range of oxidative stress-activated kinases. Tyrosine 63-66 signal transducer and activator of transcription 3 Homo sapiens 103-108 11748261-4 2001 We found that STAT3 was strongly tyrosine phosphorylated in synovial tissue of RA patients, but not those with osteoarthritis. Tyrosine 33-41 signal transducer and activator of transcription 3 Homo sapiens 14-19 11546821-4 2001 We found that Gas6 tyrosine phosphorylates STAT3 (signal transducers and activators of transcription) with concomitant translocation to the nucleus and induces STAT3-dependent transcriptional activation in cultured mesangial cells. Tyrosine 19-27 signal transducer and activator of transcription 3 Homo sapiens 160-165 11553624-1 2001 Phosphorylation of Tyr(705) and Ser(727) of signal transducer and activator of transcription 3 (STAT3) are known to be required for maximal activation by diverse stimuli. Tyrosine 19-22 signal transducer and activator of transcription 3 Homo sapiens 44-94 11553624-1 2001 Phosphorylation of Tyr(705) and Ser(727) of signal transducer and activator of transcription 3 (STAT3) are known to be required for maximal activation by diverse stimuli. Tyrosine 19-22 signal transducer and activator of transcription 3 Homo sapiens 96-101 11684091-7 2001 The Tyr-kinase inhibitor genistein and the JAK inhibitor AG490 both prevented JAK2 Tyr-phosphorylation, together with STAT1 and STAT3 Tyr-phosphorylation and binding activity. Tyrosine 4-7 signal transducer and activator of transcription 3 Homo sapiens 128-133 11598209-0 2001 Simultaneous tyrosine and serine phosphorylation of STAT3 transcription factor is involved in Rho A GTPase oncogenic transformation. Tyrosine 13-21 signal transducer and activator of transcription 3 Homo sapiens 52-57 11594781-1 2001 We previously demonstrated that exposure of CCL39 lung fibroblasts to alpha-thrombin inhibits interleukin-6 (IL-6)-induced tyrosine phosphorylation of Stat3 (signal transducers and activators of transcription 3) via activation of mitogen-activated protein (MAP) kinase kinase 1 [Bhat et al. Tyrosine 123-131 signal transducer and activator of transcription 3 Homo sapiens 151-156 11594781-1 2001 We previously demonstrated that exposure of CCL39 lung fibroblasts to alpha-thrombin inhibits interleukin-6 (IL-6)-induced tyrosine phosphorylation of Stat3 (signal transducers and activators of transcription 3) via activation of mitogen-activated protein (MAP) kinase kinase 1 [Bhat et al. Tyrosine 123-131 signal transducer and activator of transcription 3 Homo sapiens 158-210 11598209-2 2001 Here we report a novel signaling pathway whereby RhoA can efficiently modulate Stat3 transcriptional activity by inducing its simultaneous tyrosine and serine phosphorylation. Tyrosine 139-147 signal transducer and activator of transcription 3 Homo sapiens 79-84 11598209-4 2001 Furthermore, cooperation of both tyrosine as well as serine phosphorylation is necessary for full activation of Stat3. Tyrosine 33-41 signal transducer and activator of transcription 3 Homo sapiens 112-117 11598209-9 2001 Taken together, these results indicate that Stat3 is an important player in RhoA-mediated oncogenic transformation, which requires simultaneous phosphorylation at both tyrosine and serine residues by specific signaling events triggered by RhoA effectors. Tyrosine 168-176 signal transducer and activator of transcription 3 Homo sapiens 44-49 11335711-1 2001 Activation of signal transducer and activator of transcription 3 (STAT3) by interleukin-6 (IL-6) involves phosphorylation of Tyr-705 and Ser-727, both of which are critical for STAT3 transactivation. Tyrosine 125-128 signal transducer and activator of transcription 3 Homo sapiens 14-64 11490020-2 2001 In this study, we report that IL-10 can nonetheless induce Stat3 tyrosine phosphorylation and the binding of Stat1 and Stat3 to the IFN-gamma response region or the high-affinity synthetic derivative of the c-sis-inducible element in neutrophils that have been cultured for at least 3 h with LPS. Tyrosine 65-73 signal transducer and activator of transcription 3 Homo sapiens 59-64 11490020-8 2001 The ability of IL-10 to activate Stat3 tyrosine phosphorylation and SOCS-3 synthesis and to regulate IL-1 receptor antagonist and macrophage-inflammatory protein 1beta release in LPS-treated neutrophils correlated with this increased IL-10R1 expression, and was abolished by neutralizing anti-IL-10R1 and anti-IL-10R2 Abs. Tyrosine 39-47 signal transducer and activator of transcription 3 Homo sapiens 33-38 11490020-9 2001 Our results demonstrate that the capacity of neutrophils to respond to IL-10, as assessed by Stat3 tyrosine phosphorylation, SOCS-3 expression, and modulation of cytokine production, is very dependent on the level of expression of IL-10R1. Tyrosine 99-107 signal transducer and activator of transcription 3 Homo sapiens 93-98 11470914-5 2001 The activation of STAT3 requires tyrosine phosphorylation at residue 705, but is not dependent on phosphorylation of Ser-727. Tyrosine 33-41 signal transducer and activator of transcription 3 Homo sapiens 18-23 11335711-5 2001 Inhibition of PKCdelta using rottlerin (6 microm) or by overexpression of dominant negative PKCdelta demonstrates that PKCdelta kinase activity is required for STAT3 Ser-727 phosphorylation and transactivation but not for STAT3 Tyr-705 phosphorylation or nuclear import. Tyrosine 228-231 signal transducer and activator of transcription 3 Homo sapiens 160-165 11335711-7 2001 Studying the kinetics of STAT3 and PKCdelta phosphorylation in cytoplasmic and nuclear fractions revealed that STAT3 Tyr-705 phosphorylation and nuclear translocation precedes PKCdelta Thr-505 and STAT3 Ser-727 phosphorylation. Tyrosine 117-120 signal transducer and activator of transcription 3 Homo sapiens 25-30 11335711-7 2001 Studying the kinetics of STAT3 and PKCdelta phosphorylation in cytoplasmic and nuclear fractions revealed that STAT3 Tyr-705 phosphorylation and nuclear translocation precedes PKCdelta Thr-505 and STAT3 Ser-727 phosphorylation. Tyrosine 117-120 signal transducer and activator of transcription 3 Homo sapiens 111-116 11335711-7 2001 Studying the kinetics of STAT3 and PKCdelta phosphorylation in cytoplasmic and nuclear fractions revealed that STAT3 Tyr-705 phosphorylation and nuclear translocation precedes PKCdelta Thr-505 and STAT3 Ser-727 phosphorylation. Tyrosine 117-120 signal transducer and activator of transcription 3 Homo sapiens 111-116 11335711-1 2001 Activation of signal transducer and activator of transcription 3 (STAT3) by interleukin-6 (IL-6) involves phosphorylation of Tyr-705 and Ser-727, both of which are critical for STAT3 transactivation. Tyrosine 125-128 signal transducer and activator of transcription 3 Homo sapiens 66-71 11335711-1 2001 Activation of signal transducer and activator of transcription 3 (STAT3) by interleukin-6 (IL-6) involves phosphorylation of Tyr-705 and Ser-727, both of which are critical for STAT3 transactivation. Tyrosine 125-128 signal transducer and activator of transcription 3 Homo sapiens 177-182 11309383-7 2001 Activation of Tyk2 was followed by a time-dependent 2-4-fold increase in the level of tyrosine phosphorylation of signal transducers and activators of transcription 1 (STAT1), STAT2, and STAT3 and a sustained 2.5-fold increase in STAT5 tyrosine phosphorylation. Tyrosine 86-94 signal transducer and activator of transcription 3 Homo sapiens 187-192 11585385-9 2001 More specifically, we found that JAK-2/3 and STAT-3 are activated by tyrosine phosphorylation upon leptin stimulation. Tyrosine 69-77 signal transducer and activator of transcription 3 Homo sapiens 45-51 11585385-10 2001 Moreover, leptin stimulated tyrosine phosphorylation of the RNA binding protein Sam68 and its association with STAT-3. Tyrosine 28-36 signal transducer and activator of transcription 3 Homo sapiens 111-117 11473581-6 2001 We provide evidence that the cytoplasmic tyrosine residues of IFNAR-1, which remains phosphorylated by a weak IFN-alpha/beta stimulation, provide docking sites for Stat1 and Stat3 to form homo- or heterodimers following IL-6 stimulation. Tyrosine 41-49 signal transducer and activator of transcription 3 Homo sapiens 174-179 11442760-8 2001 The kinase upstream of signal transducer and activator of transcription 3, Janus kinase 1, was constitutively tyrosine-phosphorylated in WM9 cells and did not respond to interleukin-6 with increased phosphorylation. Tyrosine 110-118 signal transducer and activator of transcription 3 Homo sapiens 23-73 11309383-7 2001 Activation of Tyk2 was followed by a time-dependent 2-4-fold increase in the level of tyrosine phosphorylation of signal transducers and activators of transcription 1 (STAT1), STAT2, and STAT3 and a sustained 2.5-fold increase in STAT5 tyrosine phosphorylation. Tyrosine 236-244 signal transducer and activator of transcription 3 Homo sapiens 187-192 11429693-8 2001 Thus, the YXXQ motif regulates STAT3 activities in two ways in response to even a low concentration of IL-6: it recruits STAT3 to the receptor for tyrosine phosphorylation, and activates an unidentified H7-sensitive pathway leading to the serine phosphorylation of STAT3. Tyrosine 147-155 signal transducer and activator of transcription 3 Homo sapiens 31-36 11278353-6 2001 Kinase-inactive MEKK1 inhibits Stat3 phosphorylation on tyrosine and serine, and its transcriptional activity stimulated by epidermal growth factor and platelet-derived growth factor in different cell types. Tyrosine 56-64 signal transducer and activator of transcription 3 Homo sapiens 31-36 11278353-7 2001 In contrast, active MEKK1 induces Stat3 tyrosine and serine phosphorylation leading to a functionally active Stat3 capable of binding DNA and enhancing transcription. Tyrosine 40-48 signal transducer and activator of transcription 3 Homo sapiens 34-39 11278353-7 2001 In contrast, active MEKK1 induces Stat3 tyrosine and serine phosphorylation leading to a functionally active Stat3 capable of binding DNA and enhancing transcription. Tyrosine 40-48 signal transducer and activator of transcription 3 Homo sapiens 109-114 11378264-9 2001 Western blotting demonstrated that, in the three patients, STAT3 is constitutively phosphorylated on Tyr 705, whereas it is unphosphorylated in the other patients and in controls. Tyrosine 101-104 signal transducer and activator of transcription 3 Homo sapiens 59-64 11429693-8 2001 Thus, the YXXQ motif regulates STAT3 activities in two ways in response to even a low concentration of IL-6: it recruits STAT3 to the receptor for tyrosine phosphorylation, and activates an unidentified H7-sensitive pathway leading to the serine phosphorylation of STAT3. Tyrosine 147-155 signal transducer and activator of transcription 3 Homo sapiens 121-126 11429693-8 2001 Thus, the YXXQ motif regulates STAT3 activities in two ways in response to even a low concentration of IL-6: it recruits STAT3 to the receptor for tyrosine phosphorylation, and activates an unidentified H7-sensitive pathway leading to the serine phosphorylation of STAT3. Tyrosine 147-155 signal transducer and activator of transcription 3 Homo sapiens 121-126 11337018-5 2001 STAT3 antisense cDNA blocked the expression and IL-6-induced tyrosine phosphorylation and DNA binding of STAT3, and resulted in reduction of both IL-6-induced growth arrest at G(0)/G(1) phase and macrophage differentiation in the M1 transformants. Tyrosine 61-69 signal transducer and activator of transcription 3 Homo sapiens 0-5 11279133-2 2001 Following tyrosine phosphorylation, STAT3 proteins dimerize, translocate into the nucleus, and activate specific target genes. Tyrosine 10-18 signal transducer and activator of transcription 3 Homo sapiens 36-41 11448122-2 2001 Leptin (50-200 nM) significantly increased tyrosine phosphorylation of STAT cytoplasmic transcription factors STAT3 and STAT5b in a dose-dependent manner and produced a gel-shift with STAT3- and STAT5-specific oligonucleotides. Tyrosine 43-51 signal transducer and activator of transcription 3 Homo sapiens 110-115 11448122-2 2001 Leptin (50-200 nM) significantly increased tyrosine phosphorylation of STAT cytoplasmic transcription factors STAT3 and STAT5b in a dose-dependent manner and produced a gel-shift with STAT3- and STAT5-specific oligonucleotides. Tyrosine 43-51 signal transducer and activator of transcription 3 Homo sapiens 184-189 11294897-6 2001 Addition of a single tyrosine residue, Y724, restored its ability to stimulate cellular transformation, phosphatidylinositol 3-kinase activation, and phosphorylation of Shp2, MAPK, Stat1, and Stat3. Tyrosine 21-29 signal transducer and activator of transcription 3 Homo sapiens 192-197 11278729-10 2001 c-Src kinase activity correspondingly increased the tyrosine 705 phosphorylation and DNA binding affinity of Stat3 (but not Stat1, -5A, or -5B). Tyrosine 52-60 signal transducer and activator of transcription 3 Homo sapiens 109-114 11268587-7 2001 Phosphorylation of 985- and 1138-tyrosine of long-form leptin receptor activates SHP-2 and STAT3, respectively. Tyrosine 33-41 signal transducer and activator of transcription 3 Homo sapiens 91-96 11181699-5 2001 Among STAT family members, STAT3 was most strongly tyrosine phosphorylated in human ulcerative colitis and Crohn"s disease patients as well as in dextran sulfate sodium (DSS)-induced colitis in mice. Tyrosine 51-59 signal transducer and activator of transcription 3 Homo sapiens 27-32 11163768-3 2001 Here, we showed that, in Du-145 human prostate cancer cells, enhanced expression of BRCA1 resulted in constitutive activation of signal transducer and activator transcription factor 3 (STAT3) tyrosine and serine phosphorylation. Tyrosine 192-200 signal transducer and activator of transcription 3 Homo sapiens 129-183 11163768-3 2001 Here, we showed that, in Du-145 human prostate cancer cells, enhanced expression of BRCA1 resulted in constitutive activation of signal transducer and activator transcription factor 3 (STAT3) tyrosine and serine phosphorylation. Tyrosine 192-200 signal transducer and activator of transcription 3 Homo sapiens 185-190 11027495-6 2000 Studies on the mode of action revealed that CPDHC (1) affects the IL-6-dependent pathway by inhibiting the tyrosine phosphorylation of the STAT3 and STAT1 as well as the serine phosphorylation of the Stat3 transcription factor. Tyrosine 107-115 signal transducer and activator of transcription 3 Homo sapiens 139-144 11018044-2 2000 Phosphorylated Tyr(1138) binds STAT3 to mediate its tyrosine phosphorylation and transcriptional activation, while phosphorylated Tyr(985) binds the tyrosine phosphatase SHP-2 and reportedly mediates both activation of ERK kinases and inhibition of LRb-mediated STAT3 activation. Tyrosine 15-18 signal transducer and activator of transcription 3 Homo sapiens 31-36 11058569-5 2000 Replacement of the two tyrosine residues completely abolished STAT3 activation, although replacing either tyrosine residue alone retained it. Tyrosine 23-31 signal transducer and activator of transcription 3 Homo sapiens 62-67 10915797-8 2000 We propose that dimer stability described in vitro may be the underlying mechanism of in vivo stability of activated Stat3 proteins, regulating dephosphorylation of tyrosine 705. Tyrosine 165-173 signal transducer and activator of transcription 3 Homo sapiens 117-122 11021801-1 2000 The signal transducers and activators of transcription (STAT) transcription factors become phosphorylated on tyrosine and translocate to the nucleus after stimulation of cells with growth factors or cytokines. Tyrosine 109-117 signal transducer and activator of transcription 3 Homo sapiens 56-60 11021801-3 2000 Dominant negative Rac1 inhibited STAT3 activation by growth factors, whereas activated Rac1 stimulated STAT3 phosphorylation on both tyrosine and serine residues. Tyrosine 133-141 signal transducer and activator of transcription 3 Homo sapiens 103-108 10982829-5 2000 Our results indicate that the coiled-coil domain is essential for Stat3 recruitment to the receptor and the subsequent tyrosine phosphorylation and tyrosine phosphorylation-dependent activities, such as dimer formation, nuclear translocation, and DNA binding, stimulated by epidermal growth factor (EGF) or interleukin-6 (IL-6). Tyrosine 119-127 signal transducer and activator of transcription 3 Homo sapiens 66-71 10982829-5 2000 Our results indicate that the coiled-coil domain is essential for Stat3 recruitment to the receptor and the subsequent tyrosine phosphorylation and tyrosine phosphorylation-dependent activities, such as dimer formation, nuclear translocation, and DNA binding, stimulated by epidermal growth factor (EGF) or interleukin-6 (IL-6). Tyrosine 148-156 signal transducer and activator of transcription 3 Homo sapiens 66-71 11154231-7 2001 In the OH-2 and ANBL-6 cell lines and in a patient sample, immunoblotting showed that BMP-4 down-regulated IL-6-induced tyrosine phosphorylation of Stat3, suggesting a mechanism for the apparent antagonism between IL-6 and BMP-4. Tyrosine 120-128 signal transducer and activator of transcription 3 Homo sapiens 148-153 11141475-3 2001 In the DU-145 prostate carcinoma cell line, rhIL-11 stimulates a transient and dose-dependent increase in the tyrosine 705-phosphorylated, active form of STAT3 (STAT3 P-Tyr705), involved in the downstream signaling of IL-11R and other members of the gp130-dependent receptors. Tyrosine 110-118 signal transducer and activator of transcription 3 Homo sapiens 154-159 11141475-3 2001 In the DU-145 prostate carcinoma cell line, rhIL-11 stimulates a transient and dose-dependent increase in the tyrosine 705-phosphorylated, active form of STAT3 (STAT3 P-Tyr705), involved in the downstream signaling of IL-11R and other members of the gp130-dependent receptors. Tyrosine 110-118 signal transducer and activator of transcription 3 Homo sapiens 161-166 11141475-6 2001 Also, the tyrosine-phosphorylated, activated form of STAT3 was observed more prominently in the nuclei of cells residing in malignant glands compared to those in nonmalignant samples. Tyrosine 10-18 signal transducer and activator of transcription 3 Homo sapiens 53-58 11464862-9 2001 Tyrosine phosphorylation of gp130, Jak1, and STAT3 was observed after CNTF stimulation in these three cell lines. Tyrosine 0-8 signal transducer and activator of transcription 3 Homo sapiens 45-50 11071637-7 2000 G-CSF, but not SCF, induces the tyrosine phosphorylation of STAT1 and STAT3, transcription factors that can mediate the induction of c-fos. Tyrosine 32-40 signal transducer and activator of transcription 3 Homo sapiens 70-75 11006034-5 2000 Western blotting techniques were performed with phosphorylation-independent or phosphorylation-specific Stat 3 (tyrosine 705) antibody. Tyrosine 112-120 signal transducer and activator of transcription 3 Homo sapiens 104-110 10875236-3 2000 STAT3 was highly tyrosine phosphorylated and bound to DNA in proliferating preadipocytes, but not in growth-arrested preadipocytes or adipocytes. Tyrosine 17-25 signal transducer and activator of transcription 3 Homo sapiens 0-5 10878010-3 2000 When combined with immunoprecipitation analysis, this assay showed that p94(fer) can lead to the tyrosine phosphorylation and activation of Stat3 but not of Stat1 or Stat2. Tyrosine 97-105 signal transducer and activator of transcription 3 Homo sapiens 140-145 10903731-4 2000 Stimulation by IL-18 strongly enhanced tyrosine phosphorylation of STAT3 and of the mitogen-activated protein kinases (MAPK) p44erk-1and p42erk-2. Tyrosine 39-47 signal transducer and activator of transcription 3 Homo sapiens 67-72 10875236-4 2000 In growth-arrested confluent preadipocytes, induction of differentiation with methylisobutylxanthine, dexamethasone, and high dose insulin led to a delayed, but prolonged (3-day), increase in STAT3 tyrosine phosphorylation. Tyrosine 198-206 signal transducer and activator of transcription 3 Homo sapiens 192-197 10875236-6 2000 Insulin and methylisobutylxanthine alone, but not dexamethasone, induced STAT3 tyrosine phosphorylation in postconfluent cells. Tyrosine 79-87 signal transducer and activator of transcription 3 Homo sapiens 73-78 10777558-4 2000 Piceatannol, previously reported as a Syk/ZAP70-specific kinase inhibitor, selectively inhibits the tyrosine phosphorylation of STAT3 and STAT5, but not of STAT1 and STAT2. Tyrosine 100-108 signal transducer and activator of transcription 3 Homo sapiens 128-133 10747872-6 2000 Supporting the observation in 293T cells, endogenous STAT3 was tyrosine-phosphorylated upon IGF-I stimulation in the muscle cell line C2C12 as well as in various embryonic and adult mouse organs during different stages of development. Tyrosine 63-71 signal transducer and activator of transcription 3 Homo sapiens 53-58 10747872-7 2000 Dominant-negative JAK1 or JAK2 was able to block the IGF-IR-mediated tyrosine phosphorylation of STAT3 in 293T cells. Tyrosine 69-77 signal transducer and activator of transcription 3 Homo sapiens 97-102 10747872-5 2000 Moreover, tyrosine phosphorylation of STAT3, JAK1, and JAK2 was increased upon IGF-I stimulation of endogenous IGF-IR in 293T cells transfected with the respective STAT or JAK expression vector. Tyrosine 10-18 signal transducer and activator of transcription 3 Homo sapiens 38-43 10675331-4 2000 Also, c-Src activated by EGF treatment or expression of constitutively activated Ral-GTPase led to tyrosine phosphorylation of Stat3 and cortactin, but not Shc or subsequent Erk activation. Tyrosine 99-107 signal transducer and activator of transcription 3 Homo sapiens 127-132 10815799-4 2000 As STATs are activated by tyrosine phosphorylation, and as PDGF receptors are ligand-activated tyrosine kinases, we considered the possibility that Stat3 interacts with and is phosphorylated by PDGF receptors. Tyrosine 26-34 signal transducer and activator of transcription 3 Homo sapiens 3-8 10815799-6 2000 Moreover, Stat3 was phosphorylated on tyrosine in PDGF beta receptor immunoprecipitates of PDGF-treated but not untreated cells. Tyrosine 38-46 signal transducer and activator of transcription 3 Homo sapiens 10-15 10725745-4 2000 IL-11 at 0.1 and 10 ng/ml induces tyrosine phosphorylation of STAT3 and STAT1, respectively, although maximal responses require 50 ng/ml. Tyrosine 34-42 signal transducer and activator of transcription 3 Homo sapiens 62-67 10720495-3 2000 Conditioned medium prepared from AngII-stimulated cardiomyocytes could reproduce the tyrosine phosphorylation of STAT3 at 5 min. Tyrosine 85-93 signal transducer and activator of transcription 3 Homo sapiens 113-118 10545526-2 1999 We show here that LIF-induced JAK2 and STAT3 tyrosine phosphorylation is transient, disappearing within 20 and 40 minutes, respectively. Tyrosine 45-53 signal transducer and activator of transcription 3 Homo sapiens 39-44 10617865-6 2000 STAT3 was transcriptionally inactive in PC-3, TSU, and DU145 at the level of protein expression, tyrosine phosphorylation, and DNA binding/transcriptional activity, respectively. Tyrosine 97-105 signal transducer and activator of transcription 3 Homo sapiens 0-5 10974439-10 2000 The present data suggest that at least some of this regulation occurs at the STAT3 tyrosine phosphorylation step. Tyrosine 83-91 signal transducer and activator of transcription 3 Homo sapiens 77-82 10558875-5 1999 The observed differences of protein amounts and tyrosine phosphorylation between cytosol and nucleus, the association of STAT proteins with EGF-receptor and c-MET and with each other may all be involved in regulating the activity of the STAT transcription factors. Tyrosine 48-56 signal transducer and activator of transcription 3 Homo sapiens 237-241 10660304-2 2000 Crosstalk occurs between these pathways, because studies have shown that STAT3 requires phosphorylation on tyrosine and serine residues by independent protein kinase activities for maximal activation of target gene transcription. Tyrosine 107-115 signal transducer and activator of transcription 3 Homo sapiens 73-78 10611490-6 1999 Furthermore, IL-4 stimulation of keratinocytes also induced tyrosine phosphorylation of STAT3 which was found to bind to the phosphorylated IL-13Ralpha(1). Tyrosine 60-68 signal transducer and activator of transcription 3 Homo sapiens 88-93 11721361-5 1999 RESULTS: The increased level of Stat3 and its decreased tyrosine phosphorylation were detected in each group of gp130EX and gp190EX as compared with that in the wild type receptor subunit group. Tyrosine 56-64 signal transducer and activator of transcription 3 Homo sapiens 32-37 10542280-5 1999 The extent of STAT1 and STAT3 tyrosine and serine phosphorylation are also increased under HG conditions. Tyrosine 30-38 signal transducer and activator of transcription 3 Homo sapiens 24-29 10617281-5 1999 When Lck and STAT3 were coexpressed, STAT3 was found to have enhanced tyrosine phosphorylation and DNA binding activity. Tyrosine 70-78 signal transducer and activator of transcription 3 Homo sapiens 13-18 10617281-5 1999 When Lck and STAT3 were coexpressed, STAT3 was found to have enhanced tyrosine phosphorylation and DNA binding activity. Tyrosine 70-78 signal transducer and activator of transcription 3 Homo sapiens 37-42 10521505-3 1999 Here, we report that Ser-727 phosphorylation of Stat3 can also be induced by JNK and activated either by stress or by its upstream kinase and that various stress treatments induce serine phosphorylation of Stat3 in the absence of tyrosine phosphorylation. Tyrosine 230-238 signal transducer and activator of transcription 3 Homo sapiens 48-53 10523640-4 1999 Although the Src tyrosine kinase induces constitutive Stat3 phosphorylation on tyrosine, activation of Stat3-mediated gene regulation requires both tyrosine and serine phosphorylation of Stat3. Tyrosine 17-25 signal transducer and activator of transcription 3 Homo sapiens 54-59 10523640-4 1999 Although the Src tyrosine kinase induces constitutive Stat3 phosphorylation on tyrosine, activation of Stat3-mediated gene regulation requires both tyrosine and serine phosphorylation of Stat3. Tyrosine 17-25 signal transducer and activator of transcription 3 Homo sapiens 103-108 10523640-4 1999 Although the Src tyrosine kinase induces constitutive Stat3 phosphorylation on tyrosine, activation of Stat3-mediated gene regulation requires both tyrosine and serine phosphorylation of Stat3. Tyrosine 17-25 signal transducer and activator of transcription 3 Homo sapiens 103-108 10523640-4 1999 Although the Src tyrosine kinase induces constitutive Stat3 phosphorylation on tyrosine, activation of Stat3-mediated gene regulation requires both tyrosine and serine phosphorylation of Stat3. Tyrosine 79-87 signal transducer and activator of transcription 3 Homo sapiens 54-59 10523640-12 1999 These findings delineate a network of tyrosine and serine/threonine kinase signaling pathways that converge on Stat3 in the context of oncogenesis. Tyrosine 38-46 signal transducer and activator of transcription 3 Homo sapiens 111-116 10529382-6 1999 Conditioned medium from cultured cardiac myocytes presented the ability to tyrosine phosphorylate STAT3 through gp130 and that was further augmented with hypoxic conditioned medium. Tyrosine 75-83 signal transducer and activator of transcription 3 Homo sapiens 98-103 10521505-5 1999 We further demonstrate that JNK1, activated by its upstream kinase MKK7, negatively regulated the tyrosine phosphorylation and DNA binding and transcriptional activities of Stat3 stimulated by EGF. Tyrosine 98-106 signal transducer and activator of transcription 3 Homo sapiens 173-178 10521505-6 1999 Correspondingly, pretreatment of cells with UV reduced the EGF-stimulated tyrosine phosphorylation and phosphotyrosine-dependent activities of Stat3. Tyrosine 74-82 signal transducer and activator of transcription 3 Homo sapiens 143-148 10485875-1 1999 Signal transducers and activators of transcription (STATs) are rapidly phosphorylated on tyrosine residues in response to cytokine and growth factor stimulation of cell surface receptors. Tyrosine 89-97 signal transducer and activator of transcription 3 Homo sapiens 52-57 10515892-3 1999 Whereas IFNgamma and granulocyte colony-stimulating factor (G-CSF) are efficient inducers of STAT1 and STAT3 tyrosine phosphorylation in polymorphonuclear neutrophils (PMN), IL-10 fails to trigger STAT1 and STAT3 tyrosine and serine phosphorylation, therefore explaining its inability to induce the FcgammaRI expression in these cells. Tyrosine 109-117 signal transducer and activator of transcription 3 Homo sapiens 103-108 10485875-5 1999 We show that an inhibitor of protein phosphatases (PPs) PP1/PP2A, calyculin A, induces (i) phosphorylation of STAT3 on serine and threonine residues, (ii) inhibition of STAT3 tyrosine phosphorylation and DNA binding activity, and (iii) relocation of STAT3 from the nucleus to the cytoplasm. Tyrosine 175-183 signal transducer and activator of transcription 3 Homo sapiens 110-115 10417333-1 1999 STAT3 (signal transducer and activator of transcription 3) is a latent transcription factor that is activated by tyrosine phosphorylation (Tyr-705) in cells stimulated with cytokines or growth factors. Tyrosine 113-121 signal transducer and activator of transcription 3 Homo sapiens 0-5 10464278-6 1999 G-CSF-induced tyrosine phosphorylation of STAT 3 in Trf-R(-) cells much more than in Trf-R(+) cells. Tyrosine 14-22 signal transducer and activator of transcription 3 Homo sapiens 42-48 10446219-2 1999 Serine phosphorylation of Stat3 by mitogen-activated protein kinase has also been observed in cells responding to epidermal growth factor and shown to affect its tyrosine phosphorylation and transcriptional activity. Tyrosine 162-170 signal transducer and activator of transcription 3 Homo sapiens 26-31 10467412-5 1999 We show that inhibition of Src kinase expression or activity results in the reduction of Stat3 tyrosine phosphorylation, decline of Bcl-XL expression, and induction of cell death. Tyrosine 95-103 signal transducer and activator of transcription 3 Homo sapiens 89-94 10438903-3 1999 In contrast, IL-2 induces a rapid and transient tyrosine and serine phosphorylation of Stat3. Tyrosine 48-56 signal transducer and activator of transcription 3 Homo sapiens 87-92 10438903-6 1999 In conclusion, we show that CD3 ligation by mAb and SE triggers a rapid, PP1-sensitive tyrosine and serine phosphorylation of Stat3 in human CD4+ T cells. Tyrosine 87-95 signal transducer and activator of transcription 3 Homo sapiens 126-131 10428812-11 1999 The interferon-gamma-activated tyrosine phosphorylation of Jak2 and Stat3 was independent of the extracellular matrix. Tyrosine 31-39 signal transducer and activator of transcription 3 Homo sapiens 68-73 10446219-1 1999 Stat3 is activated by phosphorylation on Tyr-705, which leads to dimer formation, nuclear translocation, and regulation of gene expression. Tyrosine 41-44 signal transducer and activator of transcription 3 Homo sapiens 0-5 10417333-1 1999 STAT3 (signal transducer and activator of transcription 3) is a latent transcription factor that is activated by tyrosine phosphorylation (Tyr-705) in cells stimulated with cytokines or growth factors. Tyrosine 113-121 signal transducer and activator of transcription 3 Homo sapiens 7-57 10417333-1 1999 STAT3 (signal transducer and activator of transcription 3) is a latent transcription factor that is activated by tyrosine phosphorylation (Tyr-705) in cells stimulated with cytokines or growth factors. Tyrosine 139-142 signal transducer and activator of transcription 3 Homo sapiens 0-5 10417333-1 1999 STAT3 (signal transducer and activator of transcription 3) is a latent transcription factor that is activated by tyrosine phosphorylation (Tyr-705) in cells stimulated with cytokines or growth factors. Tyrosine 139-142 signal transducer and activator of transcription 3 Homo sapiens 7-57 10409622-6 1999 However, tyrosine phosphorylation of signal transducer and activator of transcription 3 (STAT3), which we show is induced by IL-13 and IL-4 in cells that express the IL-13Ralpha, was significantly reduced. Tyrosine 9-17 signal transducer and activator of transcription 3 Homo sapiens 37-87 10409622-6 1999 However, tyrosine phosphorylation of signal transducer and activator of transcription 3 (STAT3), which we show is induced by IL-13 and IL-4 in cells that express the IL-13Ralpha, was significantly reduced. Tyrosine 9-17 signal transducer and activator of transcription 3 Homo sapiens 89-94 10409622-10 1999 These data, together with the demonstration that IL-13Ralpha associates constitutively with Tyk2 and that Tyr-402 is involved in IL-13-induced phosphorylation of STAT3, suggest that the latter is mediated by Tyk2. Tyrosine 106-109 signal transducer and activator of transcription 3 Homo sapiens 162-167 10391929-4 1999 In the present study, using specific enzyme inhibitors and antisense oligonucleotides, we show that Ang II-induced tyrosine phosphorylation and nuclear translocation of STAT3 in VSMCs is mediated by p60 c-Src, whereas tyrosine dephosphorylation is mediated by calcineurin. Tyrosine 115-123 signal transducer and activator of transcription 3 Homo sapiens 169-174 10409622-11 1999 Tyrosine phosphorylation of STAT3, which was not necessary for IL-13-induced proliferation, may account for some of the effects of IL-4 and IL-13 on the function of their targets. Tyrosine 0-8 signal transducer and activator of transcription 3 Homo sapiens 28-33 10391929-4 1999 In the present study, using specific enzyme inhibitors and antisense oligonucleotides, we show that Ang II-induced tyrosine phosphorylation and nuclear translocation of STAT3 in VSMCs is mediated by p60 c-Src, whereas tyrosine dephosphorylation is mediated by calcineurin. Tyrosine 218-226 signal transducer and activator of transcription 3 Homo sapiens 169-174 10364193-0 1999 Oxidative stress triggers STAT3 tyrosine phosphorylation and nuclear translocation in human lymphocytes. Tyrosine 32-40 signal transducer and activator of transcription 3 Homo sapiens 26-31 10364193-4 1999 Moreover, anti-STAT3 antibodies specifically precipitated a protein of 92 kDa that becomes phosphorylated on tyrosine upon lymphocyte treatment with H2O2. Tyrosine 109-117 signal transducer and activator of transcription 3 Homo sapiens 15-20 10364193-7 1999 Present data suggest that H2O2 and vanadate are likely to inhibit the activity of intracellular tyrosine phosphatase(s), leading to enhanced STAT3 tyrosine phosphorylation and hence its translocation to the nucleus. Tyrosine 96-104 signal transducer and activator of transcription 3 Homo sapiens 141-146 10336453-1 1999 GM-CSF inhibits the nuclear translocation of tyrosine-phosphorylated STAT3. Tyrosine 45-53 signal transducer and activator of transcription 3 Homo sapiens 69-74 10358079-5 1999 In A431 cells, Stat1, Stat3, and Stat5, were constitutively complexed with ErbB1 and rapidly phosphorylated on tyrosine in response to EGF. Tyrosine 111-119 signal transducer and activator of transcription 3 Homo sapiens 22-27 10347203-0 1999 Activated G protein-coupled receptor induces tyrosine phosphorylation of STAT3 and agonist-selective serine phosphorylation via sustained stimulation of mitogen-activated protein kinase. Tyrosine 45-53 signal transducer and activator of transcription 3 Homo sapiens 73-78 10336453-8 1999 From the analysis of confocal laser scanning fluorescence microscopy and differential centrifugation, it was clearly demonstrated that G-CSF induced nuclear translocation of tyrosine-phosphorylated STAT3. Tyrosine 174-182 signal transducer and activator of transcription 3 Homo sapiens 198-203 10336453-10 1999 Notably, in the presence of GM-CSF, G-CSF induced the tyrosine phosphorylation of STAT3 but failed to induce the nuclear translocation of tyrosine-phosphorylated STAT3. Tyrosine 54-62 signal transducer and activator of transcription 3 Homo sapiens 82-87 10433356-5 1999 Once phosphorylated, these tyrosine residues (and their flanking peptide sequences) serve as temporary docking sites for the latent transcription factor, STAT3 (signal transducer and activator of transcription-3). Tyrosine 27-35 signal transducer and activator of transcription 3 Homo sapiens 154-159 10433356-5 1999 Once phosphorylated, these tyrosine residues (and their flanking peptide sequences) serve as temporary docking sites for the latent transcription factor, STAT3 (signal transducer and activator of transcription-3). Tyrosine 27-35 signal transducer and activator of transcription 3 Homo sapiens 161-211 10433356-6 1999 STAT3 binds to these sites via its SH2 (Src homology 2) domain, and is, in turn, tyrosine-phosphorylated by the receptor-associated JAKs. Tyrosine 81-89 signal transducer and activator of transcription 3 Homo sapiens 0-5 10336453-7 1999 On the other hand, G-CSF rapidly induced tyrosine phosphorylation of signal transducers and activators of transcription-3 (STAT3), but did not induce serine727 phosphorylation. Tyrosine 41-49 signal transducer and activator of transcription 3 Homo sapiens 69-121 10336453-7 1999 On the other hand, G-CSF rapidly induced tyrosine phosphorylation of signal transducers and activators of transcription-3 (STAT3), but did not induce serine727 phosphorylation. Tyrosine 41-49 signal transducer and activator of transcription 3 Homo sapiens 123-128 10201984-7 1999 A specific inhibitor of p38 MAP kinase completely inhibits the serine phosphorylation of STAT1 and STAT3 induced by IL-12 and IL-2 and abrogates the functional synergy between IL-12 and IL-2 without affecting STAT tyrosine phosphorylation. Tyrosine 214-222 signal transducer and activator of transcription 3 Homo sapiens 99-104 10408379-0 1999 Tyrosine phosphorylation and activation of Janus kinase 1 and STAT3 by sublytic C5b-9 complement complex in aortic endothelial cells. Tyrosine 0-8 signal transducer and activator of transcription 3 Homo sapiens 62-67 10408379-4 1999 STAT3 was rapidly tyrosine phosphorylated also by C5b-9. Tyrosine 18-26 signal transducer and activator of transcription 3 Homo sapiens 0-5 10374878-3 1999 In a previous study, we found constitutively tyrosine phosphorylated Stat3 in mycosis fungoides tumor cells. Tyrosine 45-53 signal transducer and activator of transcription 3 Homo sapiens 69-74 10068671-9 1999 IFN-alpha enhanced tyrosine phosphorylation of STAT1, STAT3, STAT4, STAT5a, and STAT5b. Tyrosine 19-27 signal transducer and activator of transcription 3 Homo sapiens 54-59 10080949-1 1999 Exposure of primary human lung fibroblasts (HLF) to interleukin-6 (IL-6) rapidly induced Stat3 (signal transducers and activators of transcription 3) tyrosine phosphorylation. Tyrosine 150-158 signal transducer and activator of transcription 3 Homo sapiens 89-94 10080949-1 1999 Exposure of primary human lung fibroblasts (HLF) to interleukin-6 (IL-6) rapidly induced Stat3 (signal transducers and activators of transcription 3) tyrosine phosphorylation. Tyrosine 150-158 signal transducer and activator of transcription 3 Homo sapiens 96-148 10080949-3 1999 Interestingly, a short pretreatment of cells with alpha-thrombin significantly inhibited IL-6-induced tyrosine phosphorylation of Stat3. Tyrosine 102-110 signal transducer and activator of transcription 3 Homo sapiens 130-135 10209066-5 1999 Treatment of cells with CNTF induced a rapid tyrosine phosphorylation of STAT3 followed by a time-dependent decay of this signal. Tyrosine 45-53 signal transducer and activator of transcription 3 Homo sapiens 73-78 10368647-2 1999 Tyrosine phosphorylation of the transcription factor STAT3 plays an important role in IL-6 cytokine family-mediated reactions. Tyrosine 0-8 signal transducer and activator of transcription 3 Homo sapiens 53-58 10368647-7 1999 Tyrosine phosphorylation of STAT3 was analyzed by Western blotting. Tyrosine 0-8 signal transducer and activator of transcription 3 Homo sapiens 28-33 10368647-12 1999 OM, but not LIF, induced rapid tyrosine phosphorylation of STAT3 in DU 145. Tyrosine 31-39 signal transducer and activator of transcription 3 Homo sapiens 59-64 10209066-6 1999 Loss of tyrosine phosphorylated STAT3 was inhibited by MG132 but did not require protein kinase C activity. Tyrosine 8-16 signal transducer and activator of transcription 3 Homo sapiens 32-37 9872331-1 1998 STAT proteins are activated by phosphorylation at specific tyrosine residue at the carboxy-terminus which is required for dimer-formation, nuclear translocation, DNA binding and transcriptional activity in cells treated with cytokines and growth factors. Tyrosine 59-67 signal transducer and activator of transcription 3 Homo sapiens 0-4 9880494-6 1999 The increase in haptoglobin gene transcription correlates with higher nuclear translocation of tyrosine-phosphorylated STAT3 and its DNA binding. Tyrosine 95-103 signal transducer and activator of transcription 3 Homo sapiens 119-124 10037026-8 1999 STAT1, STAT3, and STAT5 underwent tyrosine phosphorylation when co-expressed with Jakl and therefore are substrates for the respective Jak kinases. Tyrosine 34-42 signal transducer and activator of transcription 3 Homo sapiens 7-12 10037026-9 1999 In contrast, Jak3 co-expression resulted in tyrosine phosphorylation of STAT3 and STAT5 but not STAT1. Tyrosine 44-52 signal transducer and activator of transcription 3 Homo sapiens 72-77 9933596-8 1999 Interestingly, a receptor in which all tyrosines were mutated to phenylalanine shows normal Stat3 phosphorylation and low levels of activation of Stat1. Tyrosine 39-48 signal transducer and activator of transcription 3 Homo sapiens 92-97 9880505-3 1999 The subsequent tyrosine phosphorylation of the STATs leads to their dissociation from the receptor, dimerization, and translocation to the nucleus. Tyrosine 15-23 signal transducer and activator of transcription 3 Homo sapiens 47-52 9880505-8 1999 The specificity of binding to phosphotyrosine peptides was assayed with the tyrosine motif encompassing Tyr705 of STAT3 and with all tyrosine motifs present in the cytoplasmic tail of the signal transducer gp130. Tyrosine 37-45 signal transducer and activator of transcription 3 Homo sapiens 114-119 9880505-8 1999 The specificity of binding to phosphotyrosine peptides was assayed with the tyrosine motif encompassing Tyr705 of STAT3 and with all tyrosine motifs present in the cytoplasmic tail of the signal transducer gp130. Tyrosine 76-84 signal transducer and activator of transcription 3 Homo sapiens 114-119 9864153-0 1999 Tyrosine-dependent and -independent mechanisms of STAT3 activation by the human granulocyte colony-stimulating factor (G-CSF) receptor are differentially utilized depending on G-CSF concentration. Tyrosine 0-8 signal transducer and activator of transcription 3 Homo sapiens 50-55 9864153-7 1999 Both tyrosine-dependent and -independent mechanisms of STAT3 activation are sensitive to the Jak2 inhibitor AG-490, follow similar kinetics, and lead to transactivation of a STAT3 reporter construct, indicating functional equivalence. Tyrosine 5-13 signal transducer and activator of transcription 3 Homo sapiens 55-60 9864153-7 1999 Both tyrosine-dependent and -independent mechanisms of STAT3 activation are sensitive to the Jak2 inhibitor AG-490, follow similar kinetics, and lead to transactivation of a STAT3 reporter construct, indicating functional equivalence. Tyrosine 5-13 signal transducer and activator of transcription 3 Homo sapiens 174-179 9872331-5 1998 To unravel the mechanism of repression, we further showed that Stat3 DNA binding activity and its tyrosine phosphorylation are also inhibited under the same conditions. Tyrosine 98-106 signal transducer and activator of transcription 3 Homo sapiens 63-68 9872331-6 1998 ERK2 phosphorylates Stat3 on three serine-containing peptides and decreases its tyrosine phosphorylation induced by EGF treatment. Tyrosine 80-88 signal transducer and activator of transcription 3 Homo sapiens 20-25 9872331-8 1998 We propose that MAP kinase cascade may negatively regulate Stat3 activities by decreasing its tyrosine phosphorylation and also possibly by association. Tyrosine 94-102 signal transducer and activator of transcription 3 Homo sapiens 59-64 9845531-5 1998 This coimmunoprecipitation was specific in that Crkl immunoprecipitates do not contain STAT3, although STAT3 becomes tyrosine phosphorylated in thrombopoietin-stimulated platelets. Tyrosine 117-125 signal transducer and activator of transcription 3 Homo sapiens 103-108 9712900-5 1998 By measuring cellular responses, such as morphological changes upon differentiation, soft agar colony formation, and induction of tyrosine phosphorylation of the signal transducer and activator of transcription, STAT3, we show that signaling by IL-6, but not LIF, is significantly reduced by mutations in the Ig-like module of gp130. Tyrosine 130-138 signal transducer and activator of transcription 3 Homo sapiens 212-217 9862674-2 1998 Activation of Stat1 and Stat5 by G-CSF requires the membrane-proximal cytoplasmic domain of the receptor, including box1 and box2, while G-CSF-stimulated tyrosine phosphorylation of Stat3 also requires a region distal to box 2. Tyrosine 154-162 signal transducer and activator of transcription 3 Homo sapiens 182-187 9794795-8 1998 This phosphorylation depends on Tyr-759 in the cytoplasmatic domain of gp130, since a Tyr-759-->Phe exchange abrogates SHP2 activation and in turn leads to elevated and prolonged STAT3 and STAT1 activation as well as enhanced acute-phase protein gene induction. Tyrosine 32-35 signal transducer and activator of transcription 3 Homo sapiens 182-187 9794795-2 1998 Ligand binding to the receptor complex leads to tyrosine phosphorylation and activation of Janus kinases (Jak), phosphorylation of the signal transducing subunit gp130, followed by recruitment and phosphorylation of the signal transducer and activator of transcription factors STAT3 and STAT1 and the src homology domain (SH2)-containing protein tyrosine phosphatase (SHP2). Tyrosine 48-56 signal transducer and activator of transcription 3 Homo sapiens 277-282 9788278-6 1998 In this study, we report that PDGF- and CNTF-induced OL progenitors responded with a rapid tyrosine phosphorylation of JAK1, JAK2, STAT1alpha/beta, and STAT3. Tyrosine 91-99 signal transducer and activator of transcription 3 Homo sapiens 152-157 9738022-5 1998 In these HBx-expressing stable cells, the tyrosine phosphorylation of various STATs, including STAT3 and -5, was constitutively enhanced by HBx, and the concomitant increase in STAT-dependent DNA binding and transcriptional activation was observed. Tyrosine 42-50 signal transducer and activator of transcription 3 Homo sapiens 78-83 9738022-5 1998 In these HBx-expressing stable cells, the tyrosine phosphorylation of various STATs, including STAT3 and -5, was constitutively enhanced by HBx, and the concomitant increase in STAT-dependent DNA binding and transcriptional activation was observed. Tyrosine 42-50 signal transducer and activator of transcription 3 Homo sapiens 95-107 9712043-6 1998 Furthermore, inhibition of Jak kinases by TGF-beta was associated with a decrease in tyrosine phosphorylation of STAT3 and STAT4 proteins. Tyrosine 85-93 signal transducer and activator of transcription 3 Homo sapiens 113-118 9714332-3 1998 Using TF-1, a GM-CSF-dependent human erythroleukemia cell line, we found that GM-CSF enhanced DNA-binding and tyrosine phosphorylation of STAT3. Tyrosine 110-118 signal transducer and activator of transcription 3 Homo sapiens 138-143 9825897-11 1998 Activation of a third pathway, mediated by direct phosphorylation on tyrosine of STAT-3 plays a critical role in induction of cell polarization and formation of tubular structures. Tyrosine 69-77 signal transducer and activator of transcription 3 Homo sapiens 81-87 9692957-1 1998 A noncovalently bound dimeric form of recombinant human IL-6 interleukin-6 (IL-6D) was shown to be an antagonist for IL-6 activity, in a STAT3 tyrosine phosphorylation assay using HepG2 cells, under conditions where it does not dissociate into monomeric IL-6 (IL-6M). Tyrosine 143-151 signal transducer and activator of transcription 3 Homo sapiens 137-142 9422769-6 1998 Furthermore, GM-CSF induced the tyrosine phosphorylation of STAT3 and STAT5 but not of STAT1, STAT2, STAT4, or STAT6. Tyrosine 32-40 signal transducer and activator of transcription 3 Homo sapiens 60-65 9647732-1 1998 We present evidence that the transcription factor STAT3, derived from uninduced cells, can form stable homodimers, which are independent of the tyrosine phosphorylation status of the protein. Tyrosine 144-152 signal transducer and activator of transcription 3 Homo sapiens 50-55 9647732-4 1998 These dimers are different in structure from dimers observed after cytokine stimulation of cells, which results in tyrosine phosphorylation of STAT3 and dimerization involving the SH2 domain of STAT3. Tyrosine 115-123 signal transducer and activator of transcription 3 Homo sapiens 143-148 9647732-4 1998 These dimers are different in structure from dimers observed after cytokine stimulation of cells, which results in tyrosine phosphorylation of STAT3 and dimerization involving the SH2 domain of STAT3. Tyrosine 115-123 signal transducer and activator of transcription 3 Homo sapiens 194-199 9572990-3 1998 In addition, the transcription factor Stat-3 was tyrosine phosphorylated in the cytoplasm and subsequently translocated to the cell nucleus. Tyrosine 49-57 signal transducer and activator of transcription 3 Homo sapiens 38-44 9581833-11 1998 A similar additive relationship was observed on IFN alpha/beta- and PRL-induced Stat3 tyrosine phosphorylation. Tyrosine 86-94 signal transducer and activator of transcription 3 Homo sapiens 80-85 9531474-3 1998 STAT proteins constitute a family of transcription factors whose activation by cytokine and non-cytokine receptors leads to tyrosine phosphorylation, dimerization and translocation from the cytoplasm to the nucleus. Tyrosine 124-132 signal transducer and activator of transcription 3 Homo sapiens 0-4 9647240-5 1998 Despite a reduction in IL-6-induced STAT3 DNA binding activity in the nuclear compartment during STAT-masking, there was increased and prolonged accumulation of tyrosine-phosphorylated STAT3 in both the cytoplasmic and nuclear compartments, indicating that the capacity of tyrosine-phosphorylated STAT3 to bind DNA was reduced during STAT-masking. Tyrosine 161-169 signal transducer and activator of transcription 3 Homo sapiens 185-190 9647240-5 1998 Despite a reduction in IL-6-induced STAT3 DNA binding activity in the nuclear compartment during STAT-masking, there was increased and prolonged accumulation of tyrosine-phosphorylated STAT3 in both the cytoplasmic and nuclear compartments, indicating that the capacity of tyrosine-phosphorylated STAT3 to bind DNA was reduced during STAT-masking. Tyrosine 161-169 signal transducer and activator of transcription 3 Homo sapiens 185-190 9647240-5 1998 Despite a reduction in IL-6-induced STAT3 DNA binding activity in the nuclear compartment during STAT-masking, there was increased and prolonged accumulation of tyrosine-phosphorylated STAT3 in both the cytoplasmic and nuclear compartments, indicating that the capacity of tyrosine-phosphorylated STAT3 to bind DNA was reduced during STAT-masking. Tyrosine 273-281 signal transducer and activator of transcription 3 Homo sapiens 185-190 9647240-5 1998 Despite a reduction in IL-6-induced STAT3 DNA binding activity in the nuclear compartment during STAT-masking, there was increased and prolonged accumulation of tyrosine-phosphorylated STAT3 in both the cytoplasmic and nuclear compartments, indicating that the capacity of tyrosine-phosphorylated STAT3 to bind DNA was reduced during STAT-masking. Tyrosine 273-281 signal transducer and activator of transcription 3 Homo sapiens 185-190 9633897-6 1998 We examined the phosphorylation of STAT 3 and STAT 5 at the tyrosine residues in AML samples in which MAP kinase activity had already been found. Tyrosine 60-68 signal transducer and activator of transcription 3 Homo sapiens 35-41 9633897-9 1998 Similarly, with respect to STAT3, 17/23 cases examined (74%) showed constitutive tyrosine phosphorylation of STAT3. Tyrosine 81-89 signal transducer and activator of transcription 3 Homo sapiens 27-32 9633897-9 1998 Similarly, with respect to STAT3, 17/23 cases examined (74%) showed constitutive tyrosine phosphorylation of STAT3. Tyrosine 81-89 signal transducer and activator of transcription 3 Homo sapiens 109-114 9610741-7 1998 Moreover, signal transducers and activators of transcription STAT1 and STAT3 were also tyrosine phosphorylated, translocated to the nucleus, and bound to the putative STAT-binding DNA elements. Tyrosine 87-95 signal transducer and activator of transcription 3 Homo sapiens 71-76 9484840-3 1998 Activation of the PDGF beta-receptor led to tyrosine phosphorylation of Stat1, Stat3 and Stat5, which was accompanied by specific DNA-binding activities. Tyrosine 44-52 signal transducer and activator of transcription 3 Homo sapiens 79-84 9422769-7 1998 Tyrosine phosphorylation of STAT3 was transient reaching its maximum at 15 min. Tyrosine 0-8 signal transducer and activator of transcription 3 Homo sapiens 28-33 9343414-0 1997 STAT3 serine phosphorylation by ERK-dependent and -independent pathways negatively modulates its tyrosine phosphorylation. Tyrosine 97-105 signal transducer and activator of transcription 3 Homo sapiens 0-5 9558728-10 1998 Subsequently, the transcription factors--named STATs (signal transducers and activators of transcription)--STAT1 alpha and STAT3 are transiently recruited to the cytoplasmic domain of gp130, where they become tyrosine phosphorylated by JAK kinases. Tyrosine 209-217 signal transducer and activator of transcription 3 Homo sapiens 47-52 9558728-10 1998 Subsequently, the transcription factors--named STATs (signal transducers and activators of transcription)--STAT1 alpha and STAT3 are transiently recruited to the cytoplasmic domain of gp130, where they become tyrosine phosphorylated by JAK kinases. Tyrosine 209-217 signal transducer and activator of transcription 3 Homo sapiens 123-128 9343414-8 1997 Importantly, serine 727 phosphorylation negatively modulates STAT3 tyrosine phosphorylation, which is required for dimer formation, nuclear translocation, and the DNA binding activity of this transcriptional regulator. Tyrosine 67-75 signal transducer and activator of transcription 3 Homo sapiens 61-66 9305919-2 1997 Interleukin 2 (IL-2) induces tyrosine phosphorylation of STATs 3 and 5 (signal transducer and activator of transcription). Tyrosine 29-37 signal transducer and activator of transcription 3 Homo sapiens 57-70 9285712-1 1997 Ciliary neurotrophic factor, along with other neuropoietic cytokines, signals through the shared receptor subunit gp130 [1-3], leading to the tyrosine phosphorylation of a number of substrates [4,5], including the transcription factors STAT1 and STAT3 and the protein tyrosine phosphatase SHP-2 [6,7] [8]. Tyrosine 142-150 signal transducer and activator of transcription 3 Homo sapiens 246-251 9135582-2 1997 We have identified that hPL rapidly stimulated the tyrosine phosphorylation of at least 7 proteins including Janus Kinases (JAK1 and JAK2) and a signal transducer and activator of transcription protein (Stat3). Tyrosine 51-59 signal transducer and activator of transcription 3 Homo sapiens 203-208 9202212-3 1997 Using the tyrosine phosphorylation inhibitor, genistein, and electrophoretic mobility shift assay, we show that IL-6 and IFN-gamma induce nuclear factor STAT-3 and STAT-1 DNA-binding activity to the sis-inducible element of c-fos in a genistein-dependent pathway. Tyrosine 10-18 signal transducer and activator of transcription 3 Homo sapiens 153-159 9111365-7 1997 Furthermore, we found that although TPO induced tyrosine phosphorylation of both STAT3 and STAT5 in CMK cells, only STAT5 showed binding activities to potential STAT-binding sites that locate in the promoter region of p21 gene (p21-SIE sites), thereby leading to transactivation of p21. Tyrosine 48-56 signal transducer and activator of transcription 3 Homo sapiens 81-86 9108397-2 1997 To investigate the signaling processes induced by thrombopoietin, we have employed human platelets and recently demonstrated that thrombopoietin induces rapid tyrosine phosphorylation of Jak-2, Tyk2, Shc, Stat3, Stat5, p120(c-cbl) and other proteins in human platelets. Tyrosine 159-167 signal transducer and activator of transcription 3 Homo sapiens 205-210 9234724-1 1997 Signal transducer and transcription (STAT) factors are activated by tyrosine phosphorylation in response to a variety of cytokines, growth factors, and hormones. Tyrosine 68-76 signal transducer and activator of transcription 3 Homo sapiens 37-41 9234724-6 1997 Dimerization of Stat3 was blocked by point mutations affecting residues both in the vicinity of the tyrosine phosphorylation site (Y705) and more distant from this site, suggesting that multiple interactions are involved in dimer formation. Tyrosine 100-108 signal transducer and activator of transcription 3 Homo sapiens 16-21 9226159-4 1997 In addition, G-CSF induces the tyrosine phosphorylation of the receptor and members of the signal transducers and activators of transcription (Stat) family, including Stat3, as well as Stat1 and Stat5, depending on the cells involved. Tyrosine 31-39 signal transducer and activator of transcription 3 Homo sapiens 167-172 9164976-0 1997 Enhancement of gp130-mediated tyrosine phosphorylation of STAT3 and its DNA-binding activity in dexamethasone-treated AIDS-associated Kaposi"s sarcoma cells: selective synergy between dexamethasone and gp130-related growth factors in Kaposi"s sarcoma cell proliferation. Tyrosine 30-38 signal transducer and activator of transcription 3 Homo sapiens 58-63 9164976-7 1997 Stimulation of KS cells with sIL-6R alpha/IL-6 or OM induced rapid tyrosine phosphorylation of the transcription factor STAT3, and Dex significantly enhanced the accumulation of tyrosine-phosphorylated STAT3. Tyrosine 67-75 signal transducer and activator of transcription 3 Homo sapiens 120-125 9164976-7 1997 Stimulation of KS cells with sIL-6R alpha/IL-6 or OM induced rapid tyrosine phosphorylation of the transcription factor STAT3, and Dex significantly enhanced the accumulation of tyrosine-phosphorylated STAT3. Tyrosine 178-186 signal transducer and activator of transcription 3 Homo sapiens 120-125 9164976-7 1997 Stimulation of KS cells with sIL-6R alpha/IL-6 or OM induced rapid tyrosine phosphorylation of the transcription factor STAT3, and Dex significantly enhanced the accumulation of tyrosine-phosphorylated STAT3. Tyrosine 178-186 signal transducer and activator of transcription 3 Homo sapiens 202-207 9162009-1 1997 STAT (signal transducers and activators of transcription) proteins undergo cytokine-dependent phosphorylation on serine and tyrosine. Tyrosine 124-132 signal transducer and activator of transcription 3 Homo sapiens 0-4 9162009-3 1997 STAT3 bound to a conserved sequence in the cytoplasmic tail of the IFNAR1 chain of the receptor and underwent interferon-dependent tyrosine phosphorylation. Tyrosine 131-139 signal transducer and activator of transcription 3 Homo sapiens 0-5 9041204-15 1997 Constitutively tyrosine-phosphorylated STAT-3 homodimers were also detected in another breast cancer cell line, MDA468, which has an EGFR amplification and also has constitutive EGFR activity. Tyrosine 15-23 signal transducer and activator of transcription 3 Homo sapiens 39-45 8843416-2 1996 Using truncated and tyrosine mutants of the receptor, we show that different receptor domains are essential for the activation of Stat3 and Stat5. Tyrosine 20-28 signal transducer and activator of transcription 3 Homo sapiens 130-135 8934572-4 1996 On the other hand, the tyrosine in the YXXQ motifs essential for STAT3 activation was required for bcl-2 induction and anti-apoptosis. Tyrosine 23-31 signal transducer and activator of transcription 3 Homo sapiens 65-70 8887543-5 1996 The activated Eyk kinases specifically stimulate tyrosine phosphorylation of STAT1, STAT3 and JAK1. Tyrosine 49-57 signal transducer and activator of transcription 3 Homo sapiens 84-89 8756628-9 1996 Moreover, sequence homologies between human IL-9 receptor tyrosine 116 and tyrosines (of other receptors activating STAT3 and STAT5 were observed. Tyrosine 58-66 signal transducer and activator of transcription 3 Homo sapiens 116-121 8756628-9 1996 Moreover, sequence homologies between human IL-9 receptor tyrosine 116 and tyrosines (of other receptors activating STAT3 and STAT5 were observed. Tyrosine 75-84 signal transducer and activator of transcription 3 Homo sapiens 116-121 8695851-1 1996 To investigate the signaling processes induced by recombinant thrombopoietin, we used human platelets to recently show that thrombopoietin induces rapid tyrosine phosphorylation of Jak2, Tyk2, Shc, Stat3, Stat5, and other proteins in human platelets. Tyrosine 153-161 signal transducer and activator of transcription 3 Homo sapiens 198-203 8989665-3 1997 Cells treated for 24 h with retinoic acid (10 microM) showed a threefold increase in 125I-CNTF binding sites and were up to five times more sensitive to CNTF than untreated cells in stimulating the tyrosine phosphorylation of the transcription factor STAT3. Tyrosine 198-206 signal transducer and activator of transcription 3 Homo sapiens 251-256 8798409-1 1996 We reported recently that angiotensin II (AII) and phorbol 12-myristate 13-acetate (PMA) transiently inhibit interleukin 6 (IL-6)-stimulated tyrosine phosphorylation of signal transducers and activators of transcription 3 (Stat3) and subsequent formation of sis-inducing factor-A (SIF-A). Tyrosine 141-149 signal transducer and activator of transcription 3 Homo sapiens 169-221 8798409-1 1996 We reported recently that angiotensin II (AII) and phorbol 12-myristate 13-acetate (PMA) transiently inhibit interleukin 6 (IL-6)-stimulated tyrosine phosphorylation of signal transducers and activators of transcription 3 (Stat3) and subsequent formation of sis-inducing factor-A (SIF-A). Tyrosine 141-149 signal transducer and activator of transcription 3 Homo sapiens 223-228 8798409-9 1996 Treatment of cells with PD98059, a specific inhibitor of MAPKK1, attenuated the inhibitory effects of AII and PMA on IL-6-induced Stat3 tyrosine phosphorylation and SIF-A formation. Tyrosine 136-144 signal transducer and activator of transcription 3 Homo sapiens 130-135 8798409-10 1996 These data suggest that differences in magnitude and/or duration of activation of the MAP kinase cascade differentially affects the status of Stat3 tyrosine phosphorylation, and that MAPKK1 or a downstream intermediate is involved in the inhibition of IL-6-induced Stat3 by AII and PMA. Tyrosine 148-156 signal transducer and activator of transcription 3 Homo sapiens 142-147 8704168-4 1996 Simultaneous stimulation with SLF plus other cytokines that induce tyrosine phosphorylation of Stat3, such as interleukin-9 (IL-9) in MO7e cells or IL-6 in TF-1 cells, resulted in tyrosine phosphorylation and enhanced serine phosphorylation of Stat3. Tyrosine 67-75 signal transducer and activator of transcription 3 Homo sapiens 95-100 8666978-7 1996 This activation appears to link the cell surface receptors to the nuclear genes through a series of biochemical changes, including tyrosine phosphorylation and activation of a latent cytoplasmic transcription factor called signal transducer and activator of transcription 3 (STAT3). Tyrosine 131-139 signal transducer and activator of transcription 3 Homo sapiens 223-273 8704168-4 1996 Simultaneous stimulation with SLF plus other cytokines that induce tyrosine phosphorylation of Stat3, such as interleukin-9 (IL-9) in MO7e cells or IL-6 in TF-1 cells, resulted in tyrosine phosphorylation and enhanced serine phosphorylation of Stat3. Tyrosine 180-188 signal transducer and activator of transcription 3 Homo sapiens 95-100 8662591-7 1996 We have analyzed by mutational studies and by phosphopeptide competition assays which of the tyrosine modules of the IL-6 signal transducer gp130 are capable of recruiting either APRF or STAT1. Tyrosine 93-101 signal transducer and activator of transcription 3 Homo sapiens 179-183 8662591-8 1996 We found that two of the four tyrosine modules that are important for APRF activation also activate STAT1. Tyrosine 30-38 signal transducer and activator of transcription 3 Homo sapiens 70-74 8662591-11 1996 We compare the APRF and STAT1 activation motifs of gp130 with the STAT1 activation motif of the IFNgamma receptor and demonstrate that the specificity of activation can be changed from APRF to STAT1 and vice versa by only two point mutations within a tyrosine module. Tyrosine 251-259 signal transducer and activator of transcription 3 Homo sapiens 15-19 8662591-11 1996 We compare the APRF and STAT1 activation motifs of gp130 with the STAT1 activation motif of the IFNgamma receptor and demonstrate that the specificity of activation can be changed from APRF to STAT1 and vice versa by only two point mutations within a tyrosine module. Tyrosine 251-259 signal transducer and activator of transcription 3 Homo sapiens 185-189 8662795-9 1996 271, 12991-12998), we demonstrated that two tyrosine motifs within the cytoplasmic part of the interleukin 6 signal transducer gp130 specifically mediate APRF activation while two others can recruit both APRF and Stat1. Tyrosine 44-52 signal transducer and activator of transcription 3 Homo sapiens 154-158 8626374-2 1996 Mutation of Stat3 tyrosine 705 to phenylalanine (Y705F) inhibits IL-6-induced tyrosine phosphorylation of this Stat3 mutant in transfected HepG2 cells. Tyrosine 18-26 signal transducer and activator of transcription 3 Homo sapiens 12-17 8632998-3 1996 In the case of IL-6 and LIF, which share a signal transducing receptor gp130, STAT3 is specifically tyrosine-phosphorylated and activated by stimulation with each cytokine in various cell types. Tyrosine 100-108 signal transducer and activator of transcription 3 Homo sapiens 78-83 8675499-2 1996 Binding of IL-5 to its specific receptor activates JAK2 which leads to the tyrosine phosphorylation of STAT3 proteins. Tyrosine 75-83 signal transducer and activator of transcription 3 Homo sapiens 103-108 8675499-6 1996 We found by reconstitution of the human IL-5R in COS cells that like STAT3, STAT3beta is phosphorylated on tyrosine and binds to the pIRE from the ICAM-1 promoter after IL-5 stimulation. Tyrosine 107-115 signal transducer and activator of transcription 3 Homo sapiens 69-74 8647290-4 1996 In keeping with this result, treatment of neutrophils with G-CSF led to tyrosine phosphorylation of STAT3, as determined by immunoprecipitation followed by immunoblotting with antiphosphotyrosine antibodies. Tyrosine 72-80 signal transducer and activator of transcription 3 Homo sapiens 100-105 8626489-2 1996 We show that STAT3 undergoes IFNalpha-dependent tyrosine phosphorylation and IFNalpha treatment induces protein-DNA complexes that contain STAT3. Tyrosine 48-56 signal transducer and activator of transcription 3 Homo sapiens 13-18 8626489-2 1996 We show that STAT3 undergoes IFNalpha-dependent tyrosine phosphorylation and IFNalpha treatment induces protein-DNA complexes that contain STAT3. Tyrosine 48-56 signal transducer and activator of transcription 3 Homo sapiens 139-144 8626489-3 1996 In addition, STAT3 associates with the IFNAR-1 chain of the type I receptor in a tyrosine phosphorylation-dependent manner upon IFNalpha addition. Tyrosine 81-89 signal transducer and activator of transcription 3 Homo sapiens 13-18 8626489-4 1996 The binding of STAT3 to the IFNAR-1 chain occurs through a direct interaction between the SH2 domain-containing portion of STAT3 and the tyrosine-phosphorylated IFNAR-1 chain. Tyrosine 137-145 signal transducer and activator of transcription 3 Homo sapiens 15-20 8626489-4 1996 The binding of STAT3 to the IFNAR-1 chain occurs through a direct interaction between the SH2 domain-containing portion of STAT3 and the tyrosine-phosphorylated IFNAR-1 chain. Tyrosine 137-145 signal transducer and activator of transcription 3 Homo sapiens 123-128 8626489-5 1996 Furthermore, tyrosine-phosphorylated STAT3 bound to the IFNAR-1 chain also undergoes a secondary modification involving serine phosphorylation. Tyrosine 13-21 signal transducer and activator of transcription 3 Homo sapiens 37-42 8625884-4 1996 The JAKs, a family of intracellular kinases, and the STATs, a family of transcription factors, have both previously been shown to be tyrosine phosphorylated and activated in response to various cytokines, interferons, and growth factors in cells of non-skeletal origin. Tyrosine 133-141 signal transducer and activator of transcription 3 Homo sapiens 53-58 8625884-6 1996 In addition, two members of the STAT family, Stat1alpha and Stat3, are tyrosine phosphorylated in osteoblastic cells in culture in response to OSM. Tyrosine 71-79 signal transducer and activator of transcription 3 Homo sapiens 60-65 8657115-4 1996 Phorbol ester treatment of monocytes inhibited IFN alpha-stimulated tyrosine phosphorylation of the transcription factors Stat1alpha, Stat2, and Stat3 and of the tyrosine kinase Tyk2 but had no effect on IFN-gamma activation of Stat1alpha. Tyrosine 68-76 signal transducer and activator of transcription 3 Homo sapiens 145-150 8626374-2 1996 Mutation of Stat3 tyrosine 705 to phenylalanine (Y705F) inhibits IL-6-induced tyrosine phosphorylation of this Stat3 mutant in transfected HepG2 cells. Tyrosine 18-26 signal transducer and activator of transcription 3 Homo sapiens 111-116 8626374-3 1996 In cotransfections of HepG2 cells, the Stat3 mutant Y705F causes a reduction of the tyrosine phosphorylation of wild type Stat3-FLAG. Tyrosine 84-92 signal transducer and activator of transcription 3 Homo sapiens 39-44 8626374-3 1996 In cotransfections of HepG2 cells, the Stat3 mutant Y705F causes a reduction of the tyrosine phosphorylation of wild type Stat3-FLAG. Tyrosine 84-92 signal transducer and activator of transcription 3 Homo sapiens 122-127 8900046-4 1996 LIF stimulation of myoblasts lead to rapid phosphorylation on tyrosine of STAT3 and of ERKs 1 and 2. Tyrosine 62-70 signal transducer and activator of transcription 3 Homo sapiens 74-79 8608222-2 1996 G-CSF induces tyrosine phosphorylation of Jak1, Jak2, STAT1, and STAT3. Tyrosine 14-22 signal transducer and activator of transcription 3 Homo sapiens 65-70 8608222-11 1996 By using tyrosine-to-phenylalanine substitution mutants of G-CSF-R, we further show that tyrosine 704, present in a YXXQ consensus sequence shown to be essential for STAT3 binding to gp130, is not exclusively involved in the activation of STAT3 by G-CSF-R. Tyrosine 9-17 signal transducer and activator of transcription 3 Homo sapiens 166-171 8608222-11 1996 By using tyrosine-to-phenylalanine substitution mutants of G-CSF-R, we further show that tyrosine 704, present in a YXXQ consensus sequence shown to be essential for STAT3 binding to gp130, is not exclusively involved in the activation of STAT3 by G-CSF-R. Tyrosine 89-97 signal transducer and activator of transcription 3 Homo sapiens 166-171 8608222-11 1996 By using tyrosine-to-phenylalanine substitution mutants of G-CSF-R, we further show that tyrosine 704, present in a YXXQ consensus sequence shown to be essential for STAT3 binding to gp130, is not exclusively involved in the activation of STAT3 by G-CSF-R. Tyrosine 89-97 signal transducer and activator of transcription 3 Homo sapiens 239-244 8631962-4 1996 In the present study we demonstrate that also the signal transducers and activators of transcription (STAT) family members STAT1 p91 and STAT3 p92 and the product of the c-fps/fes protooncogene become tyrosine-phosphorylated upon GM-CSF stimulation and physically associated with both GM-CSF receptor beta common subunit and JAK2. Tyrosine 201-209 signal transducer and activator of transcription 3 Homo sapiens 137-142 8555464-0 1996 Thrombopoietin induces tyrosine phosphorylation of Stat3 and Stat5 in human blood platelets. Tyrosine 23-31 signal transducer and activator of transcription 3 Homo sapiens 51-56 8555464-6 1996 Thrombopoietin induced tyrosine phosphorylation of Stat3 and Stat5 in these cells. Tyrosine 23-31 signal transducer and activator of transcription 3 Homo sapiens 51-56 8900046-5 1996 Similarly, bFGF stimulation of myoblasts resulted in the tyrosine phosphorylation of STAT3. Tyrosine 57-65 signal transducer and activator of transcription 3 Homo sapiens 85-90 8900046-6 1996 However, unlike LIF, the bFGF induced tyrosine phosphorylation of STAT3 appeared cyclical, with recurrent peaks of phosphorylation even after prolonged exposure. Tyrosine 38-46 signal transducer and activator of transcription 3 Homo sapiens 66-71 8900046-8 1996 In differentiated myotubes, LIF treatment resulted in the tyrosine phosphorylation of both STAT3 and STAT1, but ERK phosphorylation was not detectable, and bFGF treatment did not lead to STAT1 or STAT3 tyrosine phosphorylation. Tyrosine 58-66 signal transducer and activator of transcription 3 Homo sapiens 91-96 8536642-2 1996 We have shown previously that in vivo GH administration rapidly modifies the tyrosine phosphorylation of multiple hepatic nuclear proteins, including the inducible transcription factors, Stat1, Stat3, and (in this report) Stat5, and have found that hormone treatment also rapidly alters gene transcription in the liver. Tyrosine 77-85 signal transducer and activator of transcription 3 Homo sapiens 194-199 7544303-5 1995 Additionally, TPO-induced the tyrosine phosphorylation and DNA-binding activity of STAT3. Tyrosine 30-38 signal transducer and activator of transcription 3 Homo sapiens 83-88 8566040-8 1995 Activation by tyrosine phosphorylation of the transcription factors Stat1 and APRF/Stat3 was also induced by B-S12 and B-P8, suggesting that both mAb induce homodimerization of gp130. Tyrosine 14-22 signal transducer and activator of transcription 3 Homo sapiens 78-82 8566040-8 1995 Activation by tyrosine phosphorylation of the transcription factors Stat1 and APRF/Stat3 was also induced by B-S12 and B-P8, suggesting that both mAb induce homodimerization of gp130. Tyrosine 14-22 signal transducer and activator of transcription 3 Homo sapiens 83-88 7479924-6 1995 The changes in the composition of IL-2Rs were accompanied by inhibition of IL-2-induced tyrosine phosphorylation of the Janus protein-tyrosine kinase 3 (Jak3) and signal transducers and activators of transcription called Stat3 and Stat5. Tyrosine 88-96 signal transducer and activator of transcription 3 Homo sapiens 221-226 7579336-0 1995 Distinct regions of the granulocyte colony-stimulating factor receptor are required for tyrosine phosphorylation of the signaling molecules JAK2, Stat3, and p42, p44MAPK. Tyrosine 88-96 signal transducer and activator of transcription 3 Homo sapiens 146-151 7579336-5 1995 In contrast, maximal Stat3 tyrosine phosphorylation required amino acids 96 to 183 of the G-CSF-R cytoplasmic domain, Stat3 DNA binding could occur with a receptor truncated 96 amino acids from the transmembrane domain and containing a single tyrosine residue, but was reduced in comparison with the full-length receptor. Tyrosine 27-35 signal transducer and activator of transcription 3 Homo sapiens 21-26 7579336-5 1995 In contrast, maximal Stat3 tyrosine phosphorylation required amino acids 96 to 183 of the G-CSF-R cytoplasmic domain, Stat3 DNA binding could occur with a receptor truncated 96 amino acids from the transmembrane domain and containing a single tyrosine residue, but was reduced in comparison with the full-length receptor. Tyrosine 27-35 signal transducer and activator of transcription 3 Homo sapiens 118-123 7579336-5 1995 In contrast, maximal Stat3 tyrosine phosphorylation required amino acids 96 to 183 of the G-CSF-R cytoplasmic domain, Stat3 DNA binding could occur with a receptor truncated 96 amino acids from the transmembrane domain and containing a single tyrosine residue, but was reduced in comparison with the full-length receptor. Tyrosine 243-251 signal transducer and activator of transcription 3 Homo sapiens 21-26 7579336-6 1995 Together with the tyrosine phosphorylation of Stat3, this finding suggests that additional Stat3 does not appear to be required for proliferation. Tyrosine 18-26 signal transducer and activator of transcription 3 Homo sapiens 46-51 7592760-6 1995 Moreover, endogenous STAT3 was tyrosine phosphorylated and activated in human IL-5-stimulated BaF3 cells ectopically expressing the human IL-5R (BaF3/IL5R). Tyrosine 31-39 signal transducer and activator of transcription 3 Homo sapiens 21-26 7589510-0 1995 Thrombopoietin, c-Mpl ligand, induces tyrosine phosphorylation of Tyk2, JAK2, and STAT3, and enhances agonists-induced aggregation in platelets in vitro. Tyrosine 38-46 signal transducer and activator of transcription 3 Homo sapiens 82-87 7568001-0 1995 Tyrosine phosphorylation and activation of STAT5, STAT3, and Janus kinases by interleukins 2 and 15. Tyrosine 0-8 signal transducer and activator of transcription 3 Homo sapiens 50-55 7568001-4 1995 IL-2 and IL-15 rapidly induced the tyrosine phosphorylation of STAT3 and STAT5, and DNA-binding complexes containing STAT3 and STAT5 were rapidly activated by these cytokines in T cells. Tyrosine 35-43 signal transducer and activator of transcription 3 Homo sapiens 63-68 7543512-2 1995 In this report, we show that in monocytes and T cells IL-10 stimulates tyrosine phosphorylation of the signal transducers and activators of transcription, STAT1 alpha and STAT3, in a differential manner such that the relative formation of homo- and heterodimers varies between the two cell types. Tyrosine 71-79 signal transducer and activator of transcription 3 Homo sapiens 171-176 7543416-4 1995 The activation of both JAK2 and TYK2 was dose- and time-dependent and was associated with rapid tyrosine phosphorylation of a series of STAT proteins including STAT1, STAT3, and STAT5. Tyrosine 96-104 signal transducer and activator of transcription 3 Homo sapiens 167-172 7543024-0 1995 Maximal activation of transcription by Stat1 and Stat3 requires both tyrosine and serine phosphorylation. Tyrosine 69-77 signal transducer and activator of transcription 3 Homo sapiens 49-54 7543024-1 1995 Stat1 and Stat3 are latent transcriptional factors activated initially through phosphorylation on single tyrosine residues induced by cytokine and growth factor occupation of cell surface receptors. Tyrosine 105-113 signal transducer and activator of transcription 3 Homo sapiens 10-15 7543024-4 1995 These experiments show that gene activation by Stat1 and Stat3, which obligatorily require tyrosine phosphorylation to become active, also depends for maximal activation on one or more of the many serine kinases. Tyrosine 91-99 signal transducer and activator of transcription 3 Homo sapiens 57-62 9507017-1 1998 STATs (signal transducers and activators of transcription) are transcription factors that contain SH2 domains and are activated by tyrosine phosphorylation, often in response to cytokine stimulation. Tyrosine 131-139 signal transducer and activator of transcription 3 Homo sapiens 0-5 7624343-2 1995 Receptor dimerization in turn activates receptor-associated kinases of the Jak/Tyk family, resulting in the rapid tyrosine phosphorylation of several intracellular proteins, including those of two members of the signal transducers and activators of transcription (STAT) family--STAT1 and STAT3. Tyrosine 114-122 signal transducer and activator of transcription 3 Homo sapiens 288-293 7541555-1 1995 Cytokines and growth factors induce tyrosine phosphorylation of signal transducers and activators of transcription (STATs) that directly activate gene expression. Tyrosine 36-44 signal transducer and activator of transcription 3 Homo sapiens 116-121 7541555-4 1995 Induction of this DNA-binding activity was accompanied by tyrosine phosphorylation of Stat3 and correlated with Src transformation. Tyrosine 58-66 signal transducer and activator of transcription 3 Homo sapiens 86-91 7701321-2 1995 This leads to the activation of Stat3 and Stat1, two cytoplasmic signal transducers and activators of transcription (STATs), by tyrosine phosphorylation. Tyrosine 128-136 signal transducer and activator of transcription 3 Homo sapiens 32-37 7533107-1 1995 The cytokine interleukin-6 (IL-6) rapidly activates a latent cytoplasmic transcription factor, acute-phase response factor (APRF), by tyrosine phosphorylation. Tyrosine 134-142 signal transducer and activator of transcription 3 Homo sapiens 95-122 7533107-1 1995 The cytokine interleukin-6 (IL-6) rapidly activates a latent cytoplasmic transcription factor, acute-phase response factor (APRF), by tyrosine phosphorylation. Tyrosine 134-142 signal transducer and activator of transcription 3 Homo sapiens 124-128 7512451-4 1994 The cloned APRF protein is tyrosine phosphorylated and translocated into the nucleus in response to IL-6, but not in response to IFN-gamma. Tyrosine 27-35 signal transducer and activator of transcription 3 Homo sapiens 11-15 8140422-0 1994 Stat3: a STAT family member activated by tyrosine phosphorylation in response to epidermal growth factor and interleukin-6. Tyrosine 41-49 signal transducer and activator of transcription 3 Homo sapiens 0-5 8140422-2 1994 A new family member, Stat3, becomes activated through phosphorylation on tyrosine as a DNA binding protein in response to epidermal growth factor (EGF) and interleukin-6 (IL-6) but not interferon gamma (IFN-gamma). Tyrosine 73-81 signal transducer and activator of transcription 3 Homo sapiens 21-26 33818016-14 2021 Tyrosine-phosphorylated active STAT3 was highly expressed in ASCs and decreased in MAGEC2-depleted ASCs. Tyrosine 0-8 signal transducer and activator of transcription 3 Homo sapiens 31-36 7871433-0 1995 Choice of STATs and other substrates specified by modular tyrosine-based motifs in cytokine receptors. Tyrosine 58-66 signal transducer and activator of transcription 3 Homo sapiens 10-15 7871433-4 1995 Further, these tyrosine-based motifs were modular, because addition of a Stat3-specifying motif to another cytokine receptor, that for erythropoietin, caused it to activate Stat3 in a ligand-dependent fashion. Tyrosine 15-23 signal transducer and activator of transcription 3 Homo sapiens 73-78 7871433-4 1995 Further, these tyrosine-based motifs were modular, because addition of a Stat3-specifying motif to another cytokine receptor, that for erythropoietin, caused it to activate Stat3 in a ligand-dependent fashion. Tyrosine 15-23 signal transducer and activator of transcription 3 Homo sapiens 173-178 7776967-3 1995 We now show that Stat3, a new member of the STAT (signal transducer and activator of transcription) family of transcription factors, is also phosphorylated on tyrosine residues and rapidly appears in the nucleus in response to GH. Tyrosine 159-167 signal transducer and activator of transcription 3 Homo sapiens 17-22 7528668-3 1994 Here, we report that stimulation through the IL-2R induced tyrosine phosphorylation and subsequent nuclear translocation of stat3, a newly identified member of the signal transducers and activators of transcription (STAT) family of proteins. Tyrosine 59-67 signal transducer and activator of transcription 3 Homo sapiens 124-129 7528668-8 1994 Taken together, we report that IL-2 induces tyrosine phosphorylation and subsequent nuclear translocation of stat3 and an as yet undefined 84-kDa protein in antigen-specific human T cell lines. Tyrosine 44-52 signal transducer and activator of transcription 3 Homo sapiens 109-114 7521688-4 1994 We also demonstrate that the DNA-binding protein STAT3 (also called the acute-phase response factor [APRF], activated by interleukin-6) is an early target of G-CSF-induced tyrosine phosphorylation. Tyrosine 172-180 signal transducer and activator of transcription 3 Homo sapiens 49-54 7521688-4 1994 We also demonstrate that the DNA-binding protein STAT3 (also called the acute-phase response factor [APRF], activated by interleukin-6) is an early target of G-CSF-induced tyrosine phosphorylation. Tyrosine 172-180 signal transducer and activator of transcription 3 Homo sapiens 72-99 7521688-4 1994 We also demonstrate that the DNA-binding protein STAT3 (also called the acute-phase response factor [APRF], activated by interleukin-6) is an early target of G-CSF-induced tyrosine phosphorylation. Tyrosine 172-180 signal transducer and activator of transcription 3 Homo sapiens 101-105 7521688-5 1994 G-CSF induces two DNA-binding complexes; the major complex contains tyrosine phosphorylated STAT3 protein and the minor complex appears to be a heterodimer of the STAT1 (previously p91, a component of DNA-binding complexes activated by interferons) and STAT3 proteins. Tyrosine 68-76 signal transducer and activator of transcription 3 Homo sapiens 92-97 7521688-6 1994 Antiphosphotyrosine antibody interferes with the DNA binding activity of activated STAT3, indicating that tyrosine phosphorylation of STAT3 is important for the DNA binding activity. Tyrosine 11-19 signal transducer and activator of transcription 3 Homo sapiens 83-88 7521688-6 1994 Antiphosphotyrosine antibody interferes with the DNA binding activity of activated STAT3, indicating that tyrosine phosphorylation of STAT3 is important for the DNA binding activity. Tyrosine 11-19 signal transducer and activator of transcription 3 Homo sapiens 134-139 8272872-3 1994 These cytokines caused tyrosine phosphorylation of APRF and ISGF-3 alpha p91. Tyrosine 23-31 signal transducer and activator of transcription 3 Homo sapiens 51-55 7696968-6 1994 After tyrosine specific phosphorylation of APRF, it translocates into nuclei, binds to type 2 IL-6 responsive element and induces acute phase gene expression. Tyrosine 6-14 signal transducer and activator of transcription 3 Homo sapiens 43-47 9507017-9 1998 Fes induced strong tyrosine phosphorylation of STAT3 in both expression systems, consistent with the gel-shift results. Tyrosine 19-27 signal transducer and activator of transcription 3 Homo sapiens 47-52 34563576-9 2021 Consequently, both ONC species should exert their anti-cancer action by inhibiting the pro-tumor pleiotropic STAT3 effects deriving either by its phospho-tyrosine activation or by its non-canonical signaling pathways. Tyrosine 154-162 signal transducer and activator of transcription 3 Homo sapiens 109-114 34687791-6 2022 Depletion of IL-6Ralpha abolished IL-6 induced STAT3 phosphorylation at 705th tyrosine and tumor growth of HCC cells under sorafenib treatment. Tyrosine 78-86 signal transducer and activator of transcription 3 Homo sapiens 47-52 34857889-1 2022 The oncogenic potential of the latent transcription factor signal transducer and activator of transcription (STAT)3 in many human cancers, including lung cancer, has been largely attributed to its nuclear activity as a tyrosine-phosphorylated (pY705 site) transcription factor. Tyrosine 219-227 signal transducer and activator of transcription 3 Homo sapiens 59-115 34699606-4 2021 The mechanism investigation showed that zinc induced activation of extracellular regulated protein kinases (ERK) and Janus kinase 2 (JAK2), which phosphorylated signal transduction and transcription activator 3 (Stat3) at serine 727 (S727-Stat3) and tyrosine 705 (Y705-Stat3) respectively, resulting in activation of Stat3. Tyrosine 250-258 signal transducer and activator of transcription 3 Homo sapiens 269-274 34699606-4 2021 The mechanism investigation showed that zinc induced activation of extracellular regulated protein kinases (ERK) and Janus kinase 2 (JAK2), which phosphorylated signal transduction and transcription activator 3 (Stat3) at serine 727 (S727-Stat3) and tyrosine 705 (Y705-Stat3) respectively, resulting in activation of Stat3. Tyrosine 250-258 signal transducer and activator of transcription 3 Homo sapiens 317-322 34731785-4 2021 The transcriptional function of STAT3 is canonically regulated through tyrosine phosphorylation. Tyrosine 71-79 signal transducer and activator of transcription 3 Homo sapiens 32-37 34390807-7 2021 This indicates that the two mutations neither affected the susceptibility of STAT3 to the enzymatic activity of the inactivating tyrosine phosphatase nor to the activating kinase. Tyrosine 129-137 signal transducer and activator of transcription 3 Homo sapiens 77-82 34884773-2 2021 STAT3 activation is mediated by its tyrosine phosphorylation, triggering dimerization and nuclear translocation. Tyrosine 36-44 signal transducer and activator of transcription 3 Homo sapiens 0-5 34684783-5 2021 Acacetin inhibits STAT3 phosphorylation at the tyrosine 705 residue and nuclear translocation in DU145 cells, which leads to the downregulation of STAT3 target genes. Tyrosine 47-55 signal transducer and activator of transcription 3 Homo sapiens 18-23 34260805-7 2021 This was associated with increased tyrosine-phosphorylation of transducer and activator of transcription-3 (STAT3) signaling in HSCs but not altered cell cycling. Tyrosine 35-43 signal transducer and activator of transcription 3 Homo sapiens 108-113 34684783-5 2021 Acacetin inhibits STAT3 phosphorylation at the tyrosine 705 residue and nuclear translocation in DU145 cells, which leads to the downregulation of STAT3 target genes. Tyrosine 47-55 signal transducer and activator of transcription 3 Homo sapiens 147-152 34329662-9 2021 Next, we observed that mutant IDH1 inhibitors activated STAT3 by phosphorylation at Tyr-705 position (pSTAT3-Y705) and its nuclear translocation. Tyrosine 84-87 signal transducer and activator of transcription 3 Homo sapiens 56-61 34440160-4 2021 In this review, we describe the studies performed on the main modifications affecting the activity of STAT3: phosphorylation of tyrosine 705 and serine 727; acetylation of lysine 49, 87, 601, 615, 631, 685, 707, and 709; and methylation of lysine 49, 140, and 180. Tyrosine 128-136 signal transducer and activator of transcription 3 Homo sapiens 102-107 34118143-14 2021 Y1007/8 in JAK2 and tyrosine (Tyr) residue 705 of STAT3 were phosphorylated, resulting in high expression of JAK2/STAT3, which was decreased by irradiation and baicalein treatment. Tyrosine 30-33 signal transducer and activator of transcription 3 Homo sapiens 50-55 34118143-14 2021 Y1007/8 in JAK2 and tyrosine (Tyr) residue 705 of STAT3 were phosphorylated, resulting in high expression of JAK2/STAT3, which was decreased by irradiation and baicalein treatment. Tyrosine 30-33 signal transducer and activator of transcription 3 Homo sapiens 114-119 35072342-5 2022 We utilize signaling domains derived from natural receptors (gp130, RANK, c-Fms, and Fas) and tyrosine motif-engineered artificial signaling domains that preferentially recruit target signaling molecules (Shc and STAT3) for inducing specific cell fates by the CARs. Tyrosine 94-102 signal transducer and activator of transcription 3 Homo sapiens 213-218 35200033-7 2022 Moreover, telocinobufagin was able to significantly inhibit STAT3 phosphorylation at tyrosine 705 (Y705) and its downstream targets. Tyrosine 85-93 signal transducer and activator of transcription 3 Homo sapiens 60-65 34703823-6 2021 The triple negative breast cancer cell lines, MDA-MB-231 and MDA-MB-468 displaying constitutive phosphorylation of STAT3 on the tyrosine 705 (Tyr705) residue, underwent apoptosis upon inhibition of STAT3 by 15d-PGJ2. Tyrosine 128-136 signal transducer and activator of transcription 3 Homo sapiens 115-120 34703823-6 2021 The triple negative breast cancer cell lines, MDA-MB-231 and MDA-MB-468 displaying constitutive phosphorylation of STAT3 on the tyrosine 705 (Tyr705) residue, underwent apoptosis upon inhibition of STAT3 by 15d-PGJ2. Tyrosine 128-136 signal transducer and activator of transcription 3 Homo sapiens 198-203 34703823-8 2021 Furthermore, 15d-PGJ2 inhibited interleukin-6-induced tyrosine phosphorylation of STAT3 in LNCaP cells. Tyrosine 54-62 signal transducer and activator of transcription 3 Homo sapiens 82-87 34235865-3 2021 The rate-limiting phosphorylation at a highly conserved C-terminal tyrosine residue determines nuclear translocation and transcriptional activity of STATs. Tyrosine 67-75 signal transducer and activator of transcription 3 Homo sapiens 149-154 34118143-14 2021 Y1007/8 in JAK2 and tyrosine (Tyr) residue 705 of STAT3 were phosphorylated, resulting in high expression of JAK2/STAT3, which was decreased by irradiation and baicalein treatment. Tyrosine 20-28 signal transducer and activator of transcription 3 Homo sapiens 50-55 34118143-14 2021 Y1007/8 in JAK2 and tyrosine (Tyr) residue 705 of STAT3 were phosphorylated, resulting in high expression of JAK2/STAT3, which was decreased by irradiation and baicalein treatment. Tyrosine 20-28 signal transducer and activator of transcription 3 Homo sapiens 114-119 35489748-11 2022 DMF also inhibited the constitutive tyrosine phosphorylation of STAT3 and the expression of anti-apoptotic proteins, c-IAP2 and survivin in both cells. Tyrosine 36-44 signal transducer and activator of transcription 3 Homo sapiens 64-69 35397475-7 2022 Mechanistic analyses revealed one form of activated STAT3, phospho-tyrosine-705 STAT3 (pSTAT3), was selectively upregulated in H3K27M-mutant cell lines and clinical specimens. Tyrosine 67-75 signal transducer and activator of transcription 3 Homo sapiens 52-57 35397475-7 2022 Mechanistic analyses revealed one form of activated STAT3, phospho-tyrosine-705 STAT3 (pSTAT3), was selectively upregulated in H3K27M-mutant cell lines and clinical specimens. Tyrosine 67-75 signal transducer and activator of transcription 3 Homo sapiens 80-85