PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 22749532-10 2012 Compared with the control group, postconditioning with inhalation of isoflurane or infusion of emulsified isoflurane remarkably enhanced the expression of phosphorylation of JAK2 Tyr(1007)/Tyr(1008) and STAT3 Tyr(705), but not phosphorylation of STAT1 Tyr(701). Tyrosine 179-182 Janus kinase 2 Rattus norvegicus 174-178 22749532-10 2012 Compared with the control group, postconditioning with inhalation of isoflurane or infusion of emulsified isoflurane remarkably enhanced the expression of phosphorylation of JAK2 Tyr(1007)/Tyr(1008) and STAT3 Tyr(705), but not phosphorylation of STAT1 Tyr(701). Tyrosine 189-192 Janus kinase 2 Rattus norvegicus 174-178 22749532-10 2012 Compared with the control group, postconditioning with inhalation of isoflurane or infusion of emulsified isoflurane remarkably enhanced the expression of phosphorylation of JAK2 Tyr(1007)/Tyr(1008) and STAT3 Tyr(705), but not phosphorylation of STAT1 Tyr(701). Tyrosine 189-192 Janus kinase 2 Rattus norvegicus 174-178 22749532-10 2012 Compared with the control group, postconditioning with inhalation of isoflurane or infusion of emulsified isoflurane remarkably enhanced the expression of phosphorylation of JAK2 Tyr(1007)/Tyr(1008) and STAT3 Tyr(705), but not phosphorylation of STAT1 Tyr(701). Tyrosine 189-192 Janus kinase 2 Rattus norvegicus 174-178 22095528-5 2012 The acute intracerebroventricular injection of glutamate promoted a dose-dependent increase in JAK2 tyrosine phosphorylation. Tyrosine 100-108 Janus kinase 2 Rattus norvegicus 95-99 16597920-5 2006 hGH caused tyrosine phosphorylation of Janus kinase (JAK)2 and c-Src, events inhibited by the JAK2 inhibitor AG490 or the Src kinase inhibitor PP2. Tyrosine 11-19 Janus kinase 2 Rattus norvegicus 53-58 21929289-6 2011 The tyrosine phosphorylation of JAK2 or STAT were determined by immunoblotting using specific antibodies. Tyrosine 4-12 Janus kinase 2 Rattus norvegicus 32-36 21929289-7 2011 RESULTS: High glucose markedly increased the phosphorylation of tyrosine residues of JAK2 and serine residues of STAT 1 compared with cells cultured in NG (5.5 mmol/L) with and without Ang II stimulation. Tyrosine 64-72 Janus kinase 2 Rattus norvegicus 85-89 18798867-8 2009 Tyrosine-phosphorylation of JAK2 and STAT3 and nuclear translocation of phosphorylated STAT3 were significantly decreased in the cells exposed to HG for 48 hrs. Tyrosine 0-8 Janus kinase 2 Rattus norvegicus 28-32 19063868-2 2009 We have also reported that the angiotensin II (Ang II) AT(2) receptor induced activation of SHP-1 induces the tyrosine dephosphorylation of JAK2 that results in a complete neutralization of the alpha7 nAChR-JAK2 pro-survival cascade. Tyrosine 110-118 Janus kinase 2 Rattus norvegicus 140-144 19063868-2 2009 We have also reported that the angiotensin II (Ang II) AT(2) receptor induced activation of SHP-1 induces the tyrosine dephosphorylation of JAK2 that results in a complete neutralization of the alpha7 nAChR-JAK2 pro-survival cascade. Tyrosine 110-118 Janus kinase 2 Rattus norvegicus 207-211 18611343-3 2008 Tyrosine phosphorylation of JAK2 (p-JAK2) expression was detected by immunoprecipitation and Western blotting analysis. Tyrosine 0-8 Janus kinase 2 Rattus norvegicus 28-32 18611343-3 2008 Tyrosine phosphorylation of JAK2 (p-JAK2) expression was detected by immunoprecipitation and Western blotting analysis. Tyrosine 0-8 Janus kinase 2 Rattus norvegicus 34-40 17466927-11 2007 It was concluded that AGE-induced Hsp70 protein expression and tyrosine phosphorylation are dependent on JAK2 in NRK-49F cells. Tyrosine 63-71 Janus kinase 2 Rattus norvegicus 105-109 17229938-3 2007 Upon JAK2 activation, tyrosine phosphorylation of insulin receptor substrate (IRS)-1 is detected. Tyrosine 22-30 Janus kinase 2 Rattus norvegicus 5-9 17229938-6 2007 These data indicate a new signal transduction pathway for IRS-1/PI 3-kinase/Akt/eNOS activation and ERK1/2 by means of JAK2 tyrosine phosphorylation stimulated by ACh in vessels. Tyrosine 124-132 Janus kinase 2 Rattus norvegicus 119-123 16597920-5 2006 hGH caused tyrosine phosphorylation of Janus kinase (JAK)2 and c-Src, events inhibited by the JAK2 inhibitor AG490 or the Src kinase inhibitor PP2. Tyrosine 11-19 Janus kinase 2 Rattus norvegicus 94-98 16257270-16 2005 Since previous data show that Fyn, a downstream target of Jak2, is able to phosphorylate the IP(3) receptor at Tyr 353, we believe our data suggest that Jak2 prevents the angiotensin II-mediated IP(3) receptor degradation through the activation of Fyn. Tyrosine 111-114 Janus kinase 2 Rattus norvegicus 58-62 16297319-8 2005 Immunoprecipitation and Western blot analysis were used to determine tyrosine phosphorylated JAK2 (p-JAK2). Tyrosine 69-77 Janus kinase 2 Rattus norvegicus 93-97 16257270-16 2005 Since previous data show that Fyn, a downstream target of Jak2, is able to phosphorylate the IP(3) receptor at Tyr 353, we believe our data suggest that Jak2 prevents the angiotensin II-mediated IP(3) receptor degradation through the activation of Fyn. Tyrosine 111-114 Janus kinase 2 Rattus norvegicus 153-157 15601621-8 2005 We conclude that IL-6 increases MMP-13 gene expression by activation of Jak2, resulting in tyrosine phosphorylation of the catalytic subunit of PP2A, which in turn decreases PP2A activity and prolongs c-Jun phosphorylation. Tyrosine 91-99 Janus kinase 2 Rattus norvegicus 72-76 16138568-4 2005 Furthermore, caffeic acid significantly abolished the tyrosine phosphorylation of JAK2 (decreased from 7.4 +/- 0.6-fold to 2.4 +/- 0.6-fold at 2 min) and STAT1 (decreased from 1.8 +/- 0.2-fold to 0.5 +/- 0.1-fold at 2 min) and the phosphorylation of ERK1/2 (decreased from 99.2 +/- 10.2-fold to 49.8 +/- 10.9-fold at 2 min) that were induced by Ang II. Tyrosine 54-62 Janus kinase 2 Rattus norvegicus 82-86 14722323-1 2004 We have recently provided evidence for nicotine-induced complex formation between the alpha7 nicotinic acetylcholine receptor (nAChR) and the tyrosine-phosphorylated enzyme Janus kinase 2 (JAK2) that results in subsequent activation of phosphatidylinositol-3-kinase (PI-3-K) and Akt. Tyrosine 142-150 Janus kinase 2 Rattus norvegicus 173-187 14722323-5 2004 Vanadate not only augmented the TC-1698-induced tyrosine phosphorylation of JAK2 but also blocked the Ang II neutralization of TC-1698-induced neuroprotection against Abeta (1-42)-induced cleavage of PARP. Tyrosine 48-56 Janus kinase 2 Rattus norvegicus 76-80 15644867-10 2005 Falcarindiol also abrogated the tyrosine phoshorylation of JAK2 by 82.3% at 10 min.The present study demonstrates that falcarindiol attenuated the activation of IKK and JAK contributing to the blockade of activation of NF-kappaB and Stat1, thereby leading to the suppression of iNOS expression. Tyrosine 32-40 Janus kinase 2 Rattus norvegicus 59-63 15761162-9 2005 Moreover, our results indicate a direct and positive cross-talk between insulin and leptin at the level of Janus kinase 2 and signal transducer and activator of transcription 3 tyrosine phosphorylation. Tyrosine 177-185 Janus kinase 2 Rattus norvegicus 107-176 14722323-1 2004 We have recently provided evidence for nicotine-induced complex formation between the alpha7 nicotinic acetylcholine receptor (nAChR) and the tyrosine-phosphorylated enzyme Janus kinase 2 (JAK2) that results in subsequent activation of phosphatidylinositol-3-kinase (PI-3-K) and Akt. Tyrosine 142-150 Janus kinase 2 Rattus norvegicus 189-193 12960006-3 2003 AngII induced Janus kinase 2 tyrosine phosphorylation and coimmunoprecipitation with insulin receptor substrate 1 (IRS-1) and IRS-2 as well as an increase in tyrosine phosphorylation of IRS and its association with growth factor receptor-binding protein 2. Tyrosine 29-37 Janus kinase 2 Rattus norvegicus 14-28 14657181-6 2003 We found that Ang II induced the activation of SHP-1 and that an antisense against SHP-1 not only augmented the nicotine-induced tyrosine phosphorylation of JAK2 but also blocked the Ang II neutralization of the nicotine-induced neuroprotection. Tyrosine 129-137 Janus kinase 2 Rattus norvegicus 157-161 14657181-7 2003 These results demonstrate that nicotine-induced tyrosine phosphorylation of JAK2 and neuroprotection against Abeta (1-42) in PC12 cells are blocked by Ang II via AT2 receptor-induced activation of SHP-1. Tyrosine 48-56 Janus kinase 2 Rattus norvegicus 76-80 12795614-6 2003 Thus, mitogen-induced activation of NHE-1 in VSMC is dependent upon elevated intracellular Ca(2+) and is mediated by the Jak2-dependent tyrosine phosphorylation of CaM and subsequent increased binding of CaM to NHE-1, similar to the pathway previously described for the bradykinin B(2) receptor in inner medullary collecting duct cells of the kidney [Mukhin, Y. V., et al. Tyrosine 136-144 Janus kinase 2 Rattus norvegicus 121-125 12586763-1 2003 GH stimulates the phosphorylation of tyrosine residues in the GH receptor (GHR), Janus kinase 2 (JAK2), and other signaling proteins in a transient manner that subsides within 1 h. To assess the possible roles of cytokine-induced Src homology domain 2 (SH2) (CIS/SOCS) proteins in these transient responses, we studied the expression and disposition of CIS/SOCS proteins in rat adipocytes, a physiological target of GH action. Tyrosine 37-45 Janus kinase 2 Rattus norvegicus 81-95 12586763-1 2003 GH stimulates the phosphorylation of tyrosine residues in the GH receptor (GHR), Janus kinase 2 (JAK2), and other signaling proteins in a transient manner that subsides within 1 h. To assess the possible roles of cytokine-induced Src homology domain 2 (SH2) (CIS/SOCS) proteins in these transient responses, we studied the expression and disposition of CIS/SOCS proteins in rat adipocytes, a physiological target of GH action. Tyrosine 37-45 Janus kinase 2 Rattus norvegicus 97-101 12538627-3 2003 Upon JAK2 activation, tyrosine phosphorylation of signal transducer and activator of transcription-1 (STAT-1), STAT-5b, insulin receptor substrate-1 (IRS-1), and Src homology and collagen homology (Shc) were detected. Tyrosine 22-30 Janus kinase 2 Rattus norvegicus 5-9 12674509-3 2003 Upon stimulation of JAK2 tyrosine phosphorylation, leptin induced JAK2 co-immunoprecipitation with STAT3, STAT5b, IRS-1 and IRS-2. Tyrosine 25-33 Janus kinase 2 Rattus norvegicus 20-24 12674509-3 2003 Upon stimulation of JAK2 tyrosine phosphorylation, leptin induced JAK2 co-immunoprecipitation with STAT3, STAT5b, IRS-1 and IRS-2. Tyrosine 25-33 Janus kinase 2 Rattus norvegicus 66-70 12674509-5 2003 Acutely injected insulin stimulated a mild increase in tyrosine phosphorylation of JAK2, STAT3 and STAT5b. Tyrosine 55-63 Janus kinase 2 Rattus norvegicus 83-87 12504822-5 2003 RESULTS: We showed that JAK2, STATs 1, 3, 5a and 6 and gp130 proteins are tyrosine phosphorylated as early as 5-30 min post-MI and that STATs 1, 3, and 5a remain activated up to 7 days post-MI. Tyrosine 74-82 Janus kinase 2 Rattus norvegicus 24-28 12674509-7 2003 Simultaneous treatment with both hormones yielded no change in maximal phosphorylation of STAT3, IRS-1, IRS-2 and Akt, but led to a marked increase in tyrosine phosphorylation of JAK2 and STAT5b when compared with isolated administration of insulin or leptin. Tyrosine 151-159 Janus kinase 2 Rattus norvegicus 179-183 11445068-4 2001 Insulin induced JAK2 tyrosine phosphorylation, leptin receptor phosphorylation which, in the presence of leptin, augmented the interaction between STAT3 and this receptor. Tyrosine 21-29 Janus kinase 2 Rattus norvegicus 16-20 11489940-6 2001 However, GH-induced tyrosine phosphorylation of JAK2, STAT5, and STAT3 was 75% lower in the CRF animals. Tyrosine 20-28 Janus kinase 2 Rattus norvegicus 48-52 11604224-1 2001 In vitro growth hormone (GH) stimulation of Janus kinase 2 (Jak2) tyrosine phosphorylation and activation has been detected in rat adipocytes where GH exerts both chronic diabetogenic and acute insulin-like effects but not in adipocytes where only chronic diabetogenic effects are exerted. Tyrosine 66-74 Janus kinase 2 Rattus norvegicus 44-58 11604224-1 2001 In vitro growth hormone (GH) stimulation of Janus kinase 2 (Jak2) tyrosine phosphorylation and activation has been detected in rat adipocytes where GH exerts both chronic diabetogenic and acute insulin-like effects but not in adipocytes where only chronic diabetogenic effects are exerted. Tyrosine 66-74 Janus kinase 2 Rattus norvegicus 60-64 11604224-5 2001 However, in adipocytes where GH exerts both diabetogenic and insulin-like effects, and both Jak2 and Stat5A were activated, their phosphorylation kinetics and downregulation of tyrosine phosphorylation were almost identical. Tyrosine 177-185 Janus kinase 2 Rattus norvegicus 92-96 11064147-2 2000 The results show a GH-induced and sustained phosphorylation of Jak2 and Stat5 on tyrosine residues. Tyrosine 81-89 Janus kinase 2 Rattus norvegicus 63-67 11162585-1 2001 The G-protein-coupled angiotensin II-type 1 (AT1) receptor activates the mitogen-activated protein (MAP) kinase cascade and the Janus kinase 2/signal transducers and activators of transcription (JAK2/STAT) cascade via tyrosine phosphorylation. Tyrosine 218-226 Janus kinase 2 Rattus norvegicus 128-142 11162585-1 2001 The G-protein-coupled angiotensin II-type 1 (AT1) receptor activates the mitogen-activated protein (MAP) kinase cascade and the Janus kinase 2/signal transducers and activators of transcription (JAK2/STAT) cascade via tyrosine phosphorylation. Tyrosine 218-226 Janus kinase 2 Rattus norvegicus 195-199 11231909-4 2001 JAK2 was activated rapidly in VSMCs treated with H(2)O(2), and signal transducers and activators of transcription (STAT) STAT1 and STAT3 were tyrosine-phosphorylated and translocated to the nucleus in a JAK2-dependent manner. Tyrosine 142-150 Janus kinase 2 Rattus norvegicus 0-4 11231909-4 2001 JAK2 was activated rapidly in VSMCs treated with H(2)O(2), and signal transducers and activators of transcription (STAT) STAT1 and STAT3 were tyrosine-phosphorylated and translocated to the nucleus in a JAK2-dependent manner. Tyrosine 142-150 Janus kinase 2 Rattus norvegicus 203-207 11064147-3 2000 The tyrosine phosphorylation status of Jak2 was increased in a dose-dependent manner. Tyrosine 4-12 Janus kinase 2 Rattus norvegicus 39-43 10830295-9 2000 Furthermore, our results showed that the inhibitory effect of TSH on IFNgamma signaling is caused by inhibition of tyrosine phosphorylation on STAT1, Janus kinase-1 (Jak1), and IFNgamma receptor a, but not Jak2. Tyrosine 115-123 Janus kinase 2 Rattus norvegicus 206-210 10976913-7 2000 Consistent with the effects on STAT5B phosphorylation, tyrosine-to-phenylalanine mutation of tyrosine 595 prolongs the duration of tyrosyl phosphorylation of GHR and JAK2. Tyrosine 55-63 Janus kinase 2 Rattus norvegicus 166-170 10856262-5 2000 Cardiotrophin-1-induced STAT3 tyrosine phosphorylation and binding to the St-domain were suppressed by AG490, a specific JAK2 inhibitor, which also attenuated cardiotrophin-1-stimulated angiotensinogen promoter activity. Tyrosine 30-38 Janus kinase 2 Rattus norvegicus 121-125 11032244-1 2000 Insulin induces phosphorylation and activation of JAK2 tyrosine, as well as its association with STAT1 and SHP2 in insulin-sensitive tissues of intact rats, thus demonstrating a new pathway in transduction of insulin signals. Tyrosine 55-63 Janus kinase 2 Rattus norvegicus 50-54 11032244-6 2000 In fasting and dexamethasone-treated rats, therefore, insulin-induced JAK2 tyrosine phosphorylation decreases, and the JAK2 protein expression is differentially regulated such that the insulin-induced JAK2 association with SHP2 and STAT1 shows opposite interactions with the kinase. Tyrosine 75-83 Janus kinase 2 Rattus norvegicus 70-74 10585879-4 1999 In this study we investigated the potential role of JAK-2 in insulin-mediated tyrosine phosphorylation and inactivation of PP-2A using the rat skeletal muscle cell line L6. Tyrosine 78-86 Janus kinase 2 Rattus norvegicus 52-57 10809230-3 2000 We show here that TSH induces the phosphorylation of tyrosine in the intracellular kinases Janus kinase 1 (JAK1) and -2 (JAK2) in rat thyroid cells and in Chinese hamster ovary (CHO) cells transfected with human TSH receptor (TSHR). Tyrosine 53-61 Janus kinase 2 Rattus norvegicus 121-125 10585879-6 1999 Insulin treatment did not alter JAK-2 association with PP-2A, but did increase JAK-2-mediated tyrosine phosphorylation of the PP-2A catalytic subunit and therefore inhibited PP-2A enzymic activity. Tyrosine 94-102 Janus kinase 2 Rattus norvegicus 79-84 10585879-9 1999 These observations, together with the fact that insulin rapidly activates JAK-2 in L6 cells, and that this is accompanied by an increase in tyrosine phosphorylation of PP-2A in JAK-2 immunoprecipitates, suggest that insulin controls the activation status of PP-2A by tyrosine phosphorylation via JAK-2. Tyrosine 140-148 Janus kinase 2 Rattus norvegicus 177-182 10585879-9 1999 These observations, together with the fact that insulin rapidly activates JAK-2 in L6 cells, and that this is accompanied by an increase in tyrosine phosphorylation of PP-2A in JAK-2 immunoprecipitates, suggest that insulin controls the activation status of PP-2A by tyrosine phosphorylation via JAK-2. Tyrosine 140-148 Janus kinase 2 Rattus norvegicus 177-182 10585879-9 1999 These observations, together with the fact that insulin rapidly activates JAK-2 in L6 cells, and that this is accompanied by an increase in tyrosine phosphorylation of PP-2A in JAK-2 immunoprecipitates, suggest that insulin controls the activation status of PP-2A by tyrosine phosphorylation via JAK-2. Tyrosine 267-275 Janus kinase 2 Rattus norvegicus 177-182 10585879-9 1999 These observations, together with the fact that insulin rapidly activates JAK-2 in L6 cells, and that this is accompanied by an increase in tyrosine phosphorylation of PP-2A in JAK-2 immunoprecipitates, suggest that insulin controls the activation status of PP-2A by tyrosine phosphorylation via JAK-2. Tyrosine 267-275 Janus kinase 2 Rattus norvegicus 177-182 11601336-7 1999 In addition, GH induced tyrosine phosphorylation of JAK2, a cytoplasmic protein tyrosine kinase, in cardiac myocytes. Tyrosine 24-32 Janus kinase 2 Rattus norvegicus 52-56 10432321-7 1999 Regarding the JAK/STAT pathway, AGE (100 microgram/ml) induced tyrosine phosphorylation of JAK2 (but not JAK1, JAK3 or TYK2) at 15-60 min; it also induced the tyrosine phosphorylation of STAT1 and STAT3 at 1-2 h and 0.5-4 h respectively. Tyrosine 63-71 Janus kinase 2 Rattus norvegicus 91-95 10432321-7 1999 Regarding the JAK/STAT pathway, AGE (100 microgram/ml) induced tyrosine phosphorylation of JAK2 (but not JAK1, JAK3 or TYK2) at 15-60 min; it also induced the tyrosine phosphorylation of STAT1 and STAT3 at 1-2 h and 0.5-4 h respectively. Tyrosine 159-167 Janus kinase 2 Rattus norvegicus 91-95 10432321-8 1999 Being a transcription factor, AGE also increased the DNA-binding activities of STAT1 and STAT3 AG-490 (a specific JAK2 inhibitor) (5 microM) inhibited tyrosine phosphorylation of JAK2 and the DNA-binding activities of STAT1 and STAT3. Tyrosine 151-159 Janus kinase 2 Rattus norvegicus 114-118 9886807-0 1999 Growth hormone stimulates the tyrosine kinase activity of JAK2 and induces tyrosine phosphorylation of insulin receptor substrates and Shc in rat tissues. Tyrosine 30-38 Janus kinase 2 Rattus norvegicus 58-62 9886807-6 1999 GH increased the tyrosine phosphorylation of IRS-1, IRS-2, JAK2, and Shc proteins in the liver, heart, kidney, muscle, and adipose tissue of rats. Tyrosine 17-25 Janus kinase 2 Rattus norvegicus 59-63 9892011-3 1999 Investigation of the cellular events involved in this activation/deactivation cycle using the rat liver cell line CWSV-1 established that a brief exposure to GH and the associated activation of JAK2 (Janus kinase 2) tyrosine kinase activity are both necessary and sufficient to initiate all of the downstream steps associated with STAT5b activation by tyrosine phosphorylation and the subsequent deactivation of both JAK2 kinase and STAT5b. Tyrosine 216-224 Janus kinase 2 Rattus norvegicus 194-198 9892011-3 1999 Investigation of the cellular events involved in this activation/deactivation cycle using the rat liver cell line CWSV-1 established that a brief exposure to GH and the associated activation of JAK2 (Janus kinase 2) tyrosine kinase activity are both necessary and sufficient to initiate all of the downstream steps associated with STAT5b activation by tyrosine phosphorylation and the subsequent deactivation of both JAK2 kinase and STAT5b. Tyrosine 216-224 Janus kinase 2 Rattus norvegicus 200-214 9892011-3 1999 Investigation of the cellular events involved in this activation/deactivation cycle using the rat liver cell line CWSV-1 established that a brief exposure to GH and the associated activation of JAK2 (Janus kinase 2) tyrosine kinase activity are both necessary and sufficient to initiate all of the downstream steps associated with STAT5b activation by tyrosine phosphorylation and the subsequent deactivation of both JAK2 kinase and STAT5b. Tyrosine 216-224 Janus kinase 2 Rattus norvegicus 417-421 9602424-13 1998 Selective inhibition of Jak2 kinase with AG-490 blocked formation of prolactin-inducing factor (PIF) complexes by Ang II, suggesting that Jak2 kinase was required for the tyrosine phosphorylation of Stat5 in cardiac myocytes. Tyrosine 171-179 Janus kinase 2 Rattus norvegicus 24-28 9722518-3 1998 In this study, we demonstrate that in different cell lines a particular stress, namely hyperosmolarity, results in tyrosine phosphorylation of the Janus kinases Jak1, Jak2, and Tyk2 and in the activation of STAT1 and/or STAT3. Tyrosine 115-123 Janus kinase 2 Rattus norvegicus 167-171 9790227-5 1998 GH also promoted tyrosine phosphorylation of several proteins in INS-1 cells, one of which was identified as JAK2 tyrosine kinase. Tyrosine 17-25 Janus kinase 2 Rattus norvegicus 109-113 9528967-0 1998 Desensitization of the growth hormone-induced Janus kinase 2 (Jak 2)/signal transducer and activator of transcription 5 (Stat5)-signaling pathway requires protein synthesis and phospholipase C. Signal transducers and activators of transcription (Stat) proteins are latent cytoplasmic transcription factors that are tyrosine phosphorylated by Janus kinases (Jak) in response to GH and other cytokines. Tyrosine 315-323 Janus kinase 2 Rattus norvegicus 46-60 9528967-0 1998 Desensitization of the growth hormone-induced Janus kinase 2 (Jak 2)/signal transducer and activator of transcription 5 (Stat5)-signaling pathway requires protein synthesis and phospholipase C. Signal transducers and activators of transcription (Stat) proteins are latent cytoplasmic transcription factors that are tyrosine phosphorylated by Janus kinases (Jak) in response to GH and other cytokines. Tyrosine 315-323 Janus kinase 2 Rattus norvegicus 62-67 9528967-10 1998 However, Jak2 tyrosine phosphorylation and Stat5 DNA-binding activity were prolonged for at least 4 h in the presence of cycloheximide, which suggests that the maintenance of desensitization requires ongoing protein synthesis. Tyrosine 14-22 Janus kinase 2 Rattus norvegicus 9-13 9602424-13 1998 Selective inhibition of Jak2 kinase with AG-490 blocked formation of prolactin-inducing factor (PIF) complexes by Ang II, suggesting that Jak2 kinase was required for the tyrosine phosphorylation of Stat5 in cardiac myocytes. Tyrosine 171-179 Janus kinase 2 Rattus norvegicus 138-142 9207201-6 1997 Although both JAK2 and Stat5 were phosphorylated on tyrosine upon PRL treatment, the PRL mediated inhibition of 20alpha-HSD was not reversed by either tyrosine kinase inhibitors, AG18 and genistein, but was largely reversed by the protein synthesis inhibitor cycloheximide. Tyrosine 52-60 Janus kinase 2 Rattus norvegicus 14-18 9685210-7 1998 A role has been suggested for phosphorylated receptor tyrosine residues as STAT docking sites on activated receptor-JAK complexes. Tyrosine 54-62 Janus kinase 2 Rattus norvegicus 116-119 9314595-3 1997 We have compared activation by PRL of the JAK2-STAT pathway (protein tyrosine phosphorylation and STAT5 activation) in mammary gland and liver in an in vivo rat model of induction of lactogenesis by PRL injections, and we have studied the relative proportion of both forms of the receptor in these tissues by reverse transcription-polymerase chain reaction. Tyrosine 69-77 Janus kinase 2 Rattus norvegicus 42-46 9287353-4 1997 Angiotensin II, acting through the AT1 receptor, also stimulates the Jak/STAT pathway by inducing ligand-dependent Jak2 tyrosine phosphorylation and activation. Tyrosine 120-128 Janus kinase 2 Rattus norvegicus 115-119 9287353-7 1997 The wild-type AT1A receptor can induce Jak2 tyrosine phosphorylation. Tyrosine 44-52 Janus kinase 2 Rattus norvegicus 39-43 9287353-9 1997 Competition experiments with synthetic peptides suggest that each of the YIPP amino acids, including tyrosine 319, is important in Jak2 binding to the AT1A receptor. Tyrosine 101-109 Janus kinase 2 Rattus norvegicus 131-135 9351438-4 1997 LIF (10(3) U/mL) induced rapid tyrosine phosphorylation of gp130, JAK1, JAK2, STAT1, and STAT3 but not Tyk2 or STAT2. Tyrosine 31-39 Janus kinase 2 Rattus norvegicus 72-76 9299374-9 1997 Jak2 was also tyrosine phosphorylated eight-fold by angiotensin II treatment, and phosphorylated Stat1 and Stat3 proteins co-immunoprecipitated with activated Jak2 kinase. Tyrosine 14-22 Janus kinase 2 Rattus norvegicus 0-4 8703019-0 1996 Insulin induces tyrosine phosphorylation of JAK2 in insulin-sensitive tissues of the intact rat. Tyrosine 16-24 Janus kinase 2 Rattus norvegicus 44-48 9169451-9 1997 Unexpectedly, the 5-HT2A receptor is able to activate another signaling pathway; it triggers a rapid and transient tyrosine phosphorylation of Jak2 kinase in response to serotonin. Tyrosine 115-123 Janus kinase 2 Rattus norvegicus 143-147 9169451-10 1997 Jak2 auto-phosphorylation is followed by the tyrosine phosphorylation of STAT3 (signal transducers and activators of transcription) and its translocation into the nucleus. Tyrosine 45-53 Janus kinase 2 Rattus norvegicus 0-4 8703019-2 1996 In this study, we examined the ability of insulin to stimulate JAK2 tyrosine phosphorylation in insulin-sensitive tissues of the intact rat using immunoprecipitation and immunoblotting. Tyrosine 68-76 Janus kinase 2 Rattus norvegicus 63-67 8703019-3 1996 The results demonstrate that after an infusion of insulin, JAK2 is rapidly tyrosine phosphorylated (and the kinase is activated) in the liver, adipose tissue, skeletal muscle, heart, and isolated adipocytes. Tyrosine 75-83 Janus kinase 2 Rattus norvegicus 59-63 8703019-6 1996 In two animal models of insulin resistance the regulation of JAK2 tyrosine phosphorylation after insulin infusion paralleled the phosphorylation of the insulin receptor and of IRS-1. Tyrosine 66-74 Janus kinase 2 Rattus norvegicus 61-65 8703019-7 1996 In conclusion, our data indicate that after physiological stimulation by insulin in the intact animal, JAK2 associates with the insulin receptor and is tyrosine phosphorylated in insulin-sensitive tissues in a time- and dose-dependent fashion. Tyrosine 152-160 Janus kinase 2 Rattus norvegicus 103-107 8770909-0 1996 Growth hormone stimulates tyrosine phosphorylation of JAK2 and STAT5, but not insulin receptor substrate-1 or SHC proteins in liver and skeletal muscle of normal rats in vivo. Tyrosine 26-34 Janus kinase 2 Rattus norvegicus 54-58 8892312-2 1996 Recent studies have demonstrated that ligand-induced activation of both GH and PRL receptors leads to the tyrosine phosphorylation of multiple intracellular proteins by the identical non-receptor tyrosine kinase, JAK2. Tyrosine 106-114 Janus kinase 2 Rattus norvegicus 213-217 8770909-1 1996 GH has been shown to stimulate tyrosine phosphorylation of JAK2, several STAT proteins, insulin receptor substrate-1 (IRS-1), and SHC proteins in cultured cells. Tyrosine 31-39 Janus kinase 2 Rattus norvegicus 59-63 8770909-4 1996 At a maximally effective GH dose (1.5 mg/kg body weight), phosphotyrosine antibody immunoblots demonstrated marked stimulation of the tyrosine phosphorylation of JAK2 (maximal at 5 min) and a 95,000 Mr protein (maximal at 15 min) in both liver and skeletal muscle. Tyrosine 65-73 Janus kinase 2 Rattus norvegicus 162-166 8770909-7 1996 In conclusion, GH stimulates the tyrosine phosphorylation of JAK2 and STAT5, but not IRS-1, SHC, or other STAT proteins in liver and skeletal muscle of normal rats. Tyrosine 33-41 Janus kinase 2 Rattus norvegicus 61-65 7559450-4 1995 Tyrosine phosphorylation of rJAK2 was not observed 1 day postinfection when rJAK2 was initially produced but was apparent 2 or more days postinfection when the rJAK2 level had significantly increased. Tyrosine 0-8 Janus kinase 2 Rattus norvegicus 28-33 8813644-4 1996 Immunoprecipitation assay using antibodies against Janus kinases, and the signal transducer and activator of transcription-1 (STAT1), revealed that interferon-gamma induced tyrosine phosphorylation of the just another kinase-2 (Jak2) and STAT1 alpha but did not induced the phosphorylation of Jak1, the non-receptor tyrosine kinase-2 (Tyk2) and STAT1 beta. Tyrosine 173-181 Janus kinase 2 Rattus norvegicus 228-232 8813644-5 1996 Tyrosine phosphorylation of Jak2 and STAT1 alpha induced by interferon-gamma was also inhibited by herbimycin A, while lipopolysaccharide did not induce any tyrosine phosphorylation of Janus kinases and STAT1 at all. Tyrosine 0-8 Janus kinase 2 Rattus norvegicus 28-32 7588246-4 1995 In adipocytes responsive to these effects, we found that GH induced tyrosine phosphorylation of a 114-kilodalton membrane protein, identified as the GH receptor, and a 130-kilodalton cytosolic protein, identified as JAK2. Tyrosine 68-76 Janus kinase 2 Rattus norvegicus 216-220 7588246-9 1995 We conclude that tyrosine phosphorylation of JAK2 and the GH receptor seems to be involved in the signal transduction mechanism leading to the insulin-like effects of GH in adipocytes. Tyrosine 17-25 Janus kinase 2 Rattus norvegicus 45-49 7559450-5 1995 Tyrosine phosphorylation of rJAK2(C delta 795) was not observed; further, coproduction of rJAK2(C delta 795) with rJAK2 blocked tyrosine phosphorylation of rJAK2, consistent with previously published results (Zhuang, H., Patel, S. V., He, T-C., Sonsteby, S. K., Niu, Z., and Wojchowski, D. M. (1994) J. Biol. Tyrosine 128-136 Janus kinase 2 Rattus norvegicus 90-95 7559450-4 1995 Tyrosine phosphorylation of rJAK2 was not observed 1 day postinfection when rJAK2 was initially produced but was apparent 2 or more days postinfection when the rJAK2 level had significantly increased. Tyrosine 0-8 Janus kinase 2 Rattus norvegicus 76-81 7559450-4 1995 Tyrosine phosphorylation of rJAK2 was not observed 1 day postinfection when rJAK2 was initially produced but was apparent 2 or more days postinfection when the rJAK2 level had significantly increased. Tyrosine 0-8 Janus kinase 2 Rattus norvegicus 76-81 7559450-5 1995 Tyrosine phosphorylation of rJAK2(C delta 795) was not observed; further, coproduction of rJAK2(C delta 795) with rJAK2 blocked tyrosine phosphorylation of rJAK2, consistent with previously published results (Zhuang, H., Patel, S. V., He, T-C., Sonsteby, S. K., Niu, Z., and Wojchowski, D. M. (1994) J. Biol. Tyrosine 0-8 Janus kinase 2 Rattus norvegicus 28-45 7559450-5 1995 Tyrosine phosphorylation of rJAK2(C delta 795) was not observed; further, coproduction of rJAK2(C delta 795) with rJAK2 blocked tyrosine phosphorylation of rJAK2, consistent with previously published results (Zhuang, H., Patel, S. V., He, T-C., Sonsteby, S. K., Niu, Z., and Wojchowski, D. M. (1994) J. Biol. Tyrosine 0-8 Janus kinase 2 Rattus norvegicus 28-33 7559450-5 1995 Tyrosine phosphorylation of rJAK2(C delta 795) was not observed; further, coproduction of rJAK2(C delta 795) with rJAK2 blocked tyrosine phosphorylation of rJAK2, consistent with previously published results (Zhuang, H., Patel, S. V., He, T-C., Sonsteby, S. K., Niu, Z., and Wojchowski, D. M. (1994) J. Biol. Tyrosine 128-136 Janus kinase 2 Rattus norvegicus 90-107 7559450-8 1995 Mutant (N delta 661)rJAK2 exhibited a robust tyrosine phosphorylation signal. Tyrosine 45-53 Janus kinase 2 Rattus norvegicus 20-25 7746328-4 1995 We show here that angiotensin II induces the rapid phosphorylation of tyrosine in the intracellular kinases Jak2 and Tyk2 in rat aortic smooth-muscle cells and that this phosphorylation is associated with increased activity of Jak2. Tyrosine 70-78 Janus kinase 2 Rattus norvegicus 108-112 7545168-3 1995 In this work, we examined whether tyrosines 333 and/or 338 in GHR are phosphorylated by JAK2 in response to GH. Tyrosine 34-43 Janus kinase 2 Rattus norvegicus 88-92 7746328-4 1995 We show here that angiotensin II induces the rapid phosphorylation of tyrosine in the intracellular kinases Jak2 and Tyk2 in rat aortic smooth-muscle cells and that this phosphorylation is associated with increased activity of Jak2. Tyrosine 70-78 Janus kinase 2 Rattus norvegicus 227-231 7508935-4 1994 JAK2 underwent rapid and transient tyrosine phosphorylation in response to receptor activation, reaching peak levels within 5 min of exposure to 100 nM PRL at 37 degrees C. In vitro tyrosine kinase assays using either [gamma-32P]ATP and autoradiography or unlabeled ATP combined with anti-phosphotyrosine immunoblotting, demonstrated that the activity of JAK2 was stimulated by PRL. Tyrosine 35-43 Janus kinase 2 Rattus norvegicus 0-4 7535894-10 1994 Because the tyrosine-phosphorylated transcription factor protein p91 has recently been implicated in cytokine signal transduction mediated by JAK2, we sought evidence that p91 was part of the GH-responsive binding complex. Tyrosine 12-20 Janus kinase 2 Rattus norvegicus 142-146 7508935-5 1994 Phosphoamino acid analysis of JAK2 after in vitro tyrosine kinase assay revealed that the majority of phosphate was incorporated into tyrosine residues. Tyrosine 50-58 Janus kinase 2 Rattus norvegicus 30-34 7518927-0 1994 Prolactin activates the interferon-regulated p91 transcription factor and the Jak2 kinase by tyrosine phosphorylation. Tyrosine 93-101 Janus kinase 2 Rattus norvegicus 78-82 7518927-7 1994 PRL not only induced the tyrosine phosphorylation of p91 but also induced tyrosine phosphorylation of Jak2, a tyrosine kinase required for IFN-gamma-activated gene expression. Tyrosine 74-82 Janus kinase 2 Rattus norvegicus 102-106 7510676-4 1994 However, addition of GH to cells in vitro has been shown to stimulate tyrosine phosphorylation of various intracellular proteins including mitogen-activated protein kinases (MAP kinases) and the newly described Janus kinase, JAK2. Tyrosine 70-78 Janus kinase 2 Rattus norvegicus 225-229 33942213-6 2021 RESULTS: In rat glomerular mesangial cells, VD treatment can repress the tyrosine phosphorylation of JAK2, STAT1 and STAT3. Tyrosine 73-81 Janus kinase 2 Rattus norvegicus 101-105 27068033-8 2016 MAPKs (p38 and JNK) could regulate tyrosine STAT3 phosphorylation, which suggested that the JAK2/STAT3 pathway might be the downstream of p38/JNK MAPK pathways. Tyrosine 35-43 Janus kinase 2 Rattus norvegicus 92-96