PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 29700288-4 2018 Interestingly, ATP depletion during nocodazole-induced prometaphase arrest resulted in mitotic slippage, as indicated by a reduction in mitotic cells, APC/C-dependent degradation of cyclin B1, increased cell attachment, and increased nuclear membrane reassembly. Nocodazole 36-46 cyclin B1 Homo sapiens 182-191 31249607-4 2019 In the presence of nocodazole or taxol combined with M2I-1 cell death is triggered by the premature degradation of Cyclin B1, the perturbation of the microtubule network, and an increase in the level of the pro-apoptotic protein MCL-1s combined with a marginal increase in the level of NOXA. Nocodazole 19-29 cyclin B1 Homo sapiens 115-124 24325868-7 2014 This increase in Cyclin B1 and CDC25C both together facilitated activation of cyclin B complex via dephosphorylating CDK1((T14Y15)), and resumed the G2/M transition after nocodazole synchronization. Nocodazole 171-181 cyclin B1 Homo sapiens 17-26 28112196-8 2017 Moreover, in prolonged arrest caused by nocodazole treatment, the overall levels of the CDC20-MAD2 complex are gradually, but significantly, reduced and this is associated with lower levels of cyclin B1, which brings a new insight into the mechanism of mitotic "slippage" of the arrested cells. Nocodazole 40-50 cyclin B1 Homo sapiens 193-202 22949227-0 2014 Role of cyclin B1/Cdc2 in mediating Bcl-XL phosphorylation and apoptotic cell death following nocodazole-induced mitotic arrest. Nocodazole 94-104 cyclin B1 Homo sapiens 8-17 22949227-3 2014 We recently showed that cyclin B1 and cell division cycle 2 (Cdc2) proteins are strongly up-regulated in human breast cancer cells following treatment with nocodazole (a prototypical microtubule inhibitor), and their up-regulation plays a critical role in the development of mitotic prometaphase arrest. Nocodazole 156-166 cyclin B1 Homo sapiens 24-33 22949227-4 2014 In this study, we present evidence showing that the up-regulated cyclin B1/Cdc2 complex in nocodazole-treated human breast cancer cells is also responsible for the increased phosphorylation of Bcl-2 and Bcl-XL . Nocodazole 91-101 cyclin B1 Homo sapiens 65-74 22949227-6 2014 In addition, evidence is presented to show that mitotic arrest deficient 2 (MAD2) is a key upstream mediator of the up-regulation of cyclin B1/Cdc2 as well as the subsequent increase in phosphorylationof Bcl-2 and Bcl-XL in nocodazole-treated cancer cells. Nocodazole 224-234 cyclin B1 Homo sapiens 133-142 22949227-7 2014 Together, these results reveal that the up-regulated cyclin B1/Cdc2 complex not only mediates prometaphase arrest in nocodazole-treated cells, but also activates the subsequent intrinsic cell death pathway in these cells via increased phosphorylation of Bcl-XL . Nocodazole 117-127 cyclin B1 Homo sapiens 53-62 20927403-9 2010 The exogenous expression of Cyclin B1 substantially rescued the mitotic phenotype in nocodazole cells treated with the inhibitors of transcription and translation. Nocodazole 85-95 cyclin B1 Homo sapiens 28-37 21918689-0 2011 Role of cyclin B1/Cdc2 up-regulation in the development of mitotic prometaphase arrest in human breast cancer cells treated with nocodazole. Nocodazole 129-139 cyclin B1 Homo sapiens 8-17 21918689-2 2011 Here we report our finding that treatment of MCF-7 human breast cancer cells with nocodazole, a prototypic microtubule inhibitor, results in strong up-regulation of cyclin B1 and Cdc2 levels, and their increases are required for the development of mitotic prometaphase arrest and characteristic phenotypes. Nocodazole 82-92 cyclin B1 Homo sapiens 165-174 21918689-4 2011 This early up-regulation of cyclin B1 and Cdc2 closely matched in timing the nocodazole-induced mitotic prometaphase arrest. Nocodazole 77-87 cyclin B1 Homo sapiens 28-37 21918689-5 2011 Selective knockdown of cyclin B1or Cdc2 each abrogated nocodazole-induced accumulation of prometaphase cells. Nocodazole 55-65 cyclin B1 Homo sapiens 23-32 21918689-6 2011 The nocodazole-induced prometaphase arrest was also abrogated by pre-treatment of cells with roscovitine, an inhibitor of cyclin-dependent kinases, or with cycloheximide, a protein synthesis inhibitor that was found to suppress cyclin B1 and Cdc2 up-regulation. Nocodazole 4-14 cyclin B1 Homo sapiens 228-237 21918689-7 2011 In addition, we found that MAD2 knockdown abrogated nocodazole-induced accumulation of cyclin B1 and Cdc2 proteins, which was accompanied by an attenuation of nocodazole-induced prometaphase arrest. Nocodazole 52-62 cyclin B1 Homo sapiens 87-96 15302583-6 2004 When the cells were synchronized by nocodazole, TIS21 overexpressers inhibited degradations of cyclin A and cyclin B1 in 3 h after release from the synchronization. Nocodazole 36-46 cyclin B1 Homo sapiens 108-117 20660152-10 2010 Consistent with this possibility, we found that cyclin B1- and cyclin B1/B2-knockdown cells had difficulty in maintaining a mitotic arrest in the presence of nocodazole. Nocodazole 158-168 cyclin B1 Homo sapiens 48-57 20660152-10 2010 Consistent with this possibility, we found that cyclin B1- and cyclin B1/B2-knockdown cells had difficulty in maintaining a mitotic arrest in the presence of nocodazole. Nocodazole 158-168 cyclin B1 Homo sapiens 63-72 18418077-6 2008 The massive apoptosis triggered by nocodazole treatment requires the continuous activation of cyclin B1-CDK1 and is antagonized by premature mitotic slippage. Nocodazole 35-45 cyclin B1 Homo sapiens 94-103 18414058-9 2008 Nocodazole treated, cyclin B1-depleted HeLa cells arrested but exited that arrest at higher rates than controls, suggesting that the duration of the spindle checkpoint was affected. Nocodazole 0-10 cyclin B1 Homo sapiens 20-29 10623472-6 2000 In addition, cell extracts prepared from C. cohnii in G1 phase and G2/M phase (or nocodazole treated) were able to activate and inhibit, respectively, the degradation of exogenous human cyclin B1 in vitro. Nocodazole 82-92 cyclin B1 Homo sapiens 186-195 10194549-3 1999 The nocodazole-synchronized cells progressed from M to G1 phase in the absence of the drug, which was accompanied by a decrease of cyclin B1 protein expression, disappearance of the complex formation of CDC2 with cyclin B1 and reduction of the kinase activity. Nocodazole 4-14 cyclin B1 Homo sapiens 131-140 10194549-3 1999 The nocodazole-synchronized cells progressed from M to G1 phase in the absence of the drug, which was accompanied by a decrease of cyclin B1 protein expression, disappearance of the complex formation of CDC2 with cyclin B1 and reduction of the kinase activity. Nocodazole 4-14 cyclin B1 Homo sapiens 213-222 32181475-6 2020 Knockdown of GINS2 reverses the effect of nocodazole on the levels of CDK1 and cyclin-B1. Nocodazole 42-52 cyclin B1 Homo sapiens 79-88 31906029-4 2019 Induction of G2/M arrest by TAX and NOC with increases in phosphorylated Cdc25C and cyclin B1 protein were observed in human colon cancer cells. Nocodazole 36-39 cyclin B1 Homo sapiens 84-93