PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 32394671-10 2020 Lastly, aphidicolin increased acetylcholine-induced vessel relaxation in rat aortas, which was accompanied by increased p-ATM-Ser1981, p-Akt-Ser473, p-CREB-Ser133, and eNOS expression. Acetylcholine 30-43 nitric oxide synthase 3 Rattus norvegicus 168-172 33921777-8 2021 These findings suggest that the administration of bee bread ameliorates the impaired vasorelaxation response to ACh by improving eNOS/NO/cGMP-signaling pathway in obese rats, suggesting its vascular therapeutic role. Acetylcholine 112-115 nitric oxide synthase 3 Rattus norvegicus 129-133 32956647-6 2021 The decreased Ach-induced vasodilation was related to decreased nitric oxide (NO) production in the endothelium, not NO sensitivity in vascular smooth muscle because sodium nitroprusside (SNP)-mediated vasodilation was preserved; there was decreased NO production and lower eNOS phosphorylation in male DMO. Acetylcholine 14-17 nitric oxide synthase 3 Rattus norvegicus 274-278 29527953-7 2018 Acute exposure of aortic segments to high glucose causes a reduction in acetylcholine-induced relaxation in association with suppression of Akt/eNOS pathway, as well as several genes in insulin signaling pathway. Acetylcholine 72-85 nitric oxide synthase 3 Rattus norvegicus 144-148 27474265-9 2016 An increase in eNOS signalling during the active period is responsible for this time-of-day difference in response to phenylephrine and acetylcholine and correlates with the large increase in eNOS expression (mRNA and protein) during the active period, possibly driven by the presence of a functioning peripheral circadian clock. Acetylcholine 136-149 nitric oxide synthase 3 Rattus norvegicus 15-19 21167958-4 2011 Under inhibition of constitutive NO-synthases (cNOS), the relaxation induced by acetylcholine was greater in the aortic rings of rats submitted to CLP compared with sham-operated rat aortic rings. Acetylcholine 80-93 nitric oxide synthase 3 Rattus norvegicus 47-51 22361244-8 2012 There was a significant positive correlation between eNOS expression and maximal relaxation response (Emax) to acetylcholine. Acetylcholine 111-124 nitric oxide synthase 3 Rattus norvegicus 53-57 21167958-6 2011 Acetylcholine-induced NO production was lower in the endothelial cells from the aorta of CLP rats compared with sham rat aorta, but the protein expression of the cNOS was not altered. Acetylcholine 0-13 nitric oxide synthase 3 Rattus norvegicus 162-166 17229938-0 2007 Defective insulin and acetylcholine induction of endothelial cell-nitric oxide synthase through insulin receptor substrate/Akt signaling pathway in aorta of obese rats. Acetylcholine 22-35 nitric oxide synthase 3 Rattus norvegicus 49-87 20935150-8 2010 Importantly, inhibition of either PI3K or eNOS attenuated exercise-induced restoration of the dilatation function and blocked PI3K, Akt, and eNOS phosphorylation by ACh in the mesenteric arteries. Acetylcholine 165-168 nitric oxide synthase 3 Rattus norvegicus 42-46 20935150-8 2010 Importantly, inhibition of either PI3K or eNOS attenuated exercise-induced restoration of the dilatation function and blocked PI3K, Akt, and eNOS phosphorylation by ACh in the mesenteric arteries. Acetylcholine 165-168 nitric oxide synthase 3 Rattus norvegicus 141-145 17229938-4 2007 In addition, ACh induces JAK2/IRS-1 and IRS-1/phosphatidylinositol (PI) 3-kinase associations, downstream activation of Akt/protein kinase B, endothelial cell-nitric oxide synthase (eNOS), and extracellular signal-regulated kinase (ERK)-1/2. Acetylcholine 13-16 nitric oxide synthase 3 Rattus norvegicus 142-180 17229938-4 2007 In addition, ACh induces JAK2/IRS-1 and IRS-1/phosphatidylinositol (PI) 3-kinase associations, downstream activation of Akt/protein kinase B, endothelial cell-nitric oxide synthase (eNOS), and extracellular signal-regulated kinase (ERK)-1/2. Acetylcholine 13-16 nitric oxide synthase 3 Rattus norvegicus 182-186 17229938-5 2007 The pharmacological blockade of JAK2 or PI 3-kinase reduced ACh-stimulated eNOS phosphorylation, NOS activity, and aorta relaxation. Acetylcholine 60-63 nitric oxide synthase 3 Rattus norvegicus 75-79 17229938-6 2007 These data indicate a new signal transduction pathway for IRS-1/PI 3-kinase/Akt/eNOS activation and ERK1/2 by means of JAK2 tyrosine phosphorylation stimulated by ACh in vessels. Acetylcholine 163-166 nitric oxide synthase 3 Rattus norvegicus 80-84 17229938-7 2007 Moreover, we demonstrate that in aorta of obese rats (high-fat diet), there is an impairment in the insulin- and ACh-stimulated IRS-1/PI 3-kinase pathway, leading to reduced activation with lower protein levels of eNOS associated with a hyperactivated ERK/mitogen-activated protein kinase pathway. Acetylcholine 113-116 nitric oxide synthase 3 Rattus norvegicus 214-218 17229938-8 2007 These results suggest that in aorta of obese rats, there not only is insulin resistance but also ACh resistance, probably mediated by a common signaling pathway that controls the activity and the protein levels of eNOS. Acetylcholine 97-100 nitric oxide synthase 3 Rattus norvegicus 214-218 18386218-2 2008 METHODS: We examined the reactivity of cerebral arterioles in adult and aged Fisher-344 rats to endothelial nitric oxide synthase (eNOS)-dependent (acetylcholine and adenosine diphosphate [ADP]) and-independent (nitroglycerin) agonists before and during application of tempol, apocynin, and diphenyleneiodonium chloride (DPI). Acetylcholine 148-161 nitric oxide synthase 3 Rattus norvegicus 96-129 18386218-2 2008 METHODS: We examined the reactivity of cerebral arterioles in adult and aged Fisher-344 rats to endothelial nitric oxide synthase (eNOS)-dependent (acetylcholine and adenosine diphosphate [ADP]) and-independent (nitroglycerin) agonists before and during application of tempol, apocynin, and diphenyleneiodonium chloride (DPI). Acetylcholine 148-161 nitric oxide synthase 3 Rattus norvegicus 131-135 16799985-8 2006 In conclusion, the impaired response to acetylcholine of livers with cirrhosis is modulated by a reduced availability of the eNOS cofactor, tetrahydrobiopterin. Acetylcholine 40-53 nitric oxide synthase 3 Rattus norvegicus 125-129 17109653-8 2006 prevented hypertension-induced attenuation of acetylcholine-induced endothelium-dependent relaxation, impairment of vascular endothelial lining, decrease in expression of mRNA for endothelial nitric oxide synthase (eNOS), serum nitrite/nitrate concentration and increase in expression of mRNA for p22phox, superoxide anion and serum TBARS. Acetylcholine 46-59 nitric oxide synthase 3 Rattus norvegicus 180-213 17109653-8 2006 prevented hypertension-induced attenuation of acetylcholine-induced endothelium-dependent relaxation, impairment of vascular endothelial lining, decrease in expression of mRNA for endothelial nitric oxide synthase (eNOS), serum nitrite/nitrate concentration and increase in expression of mRNA for p22phox, superoxide anion and serum TBARS. Acetylcholine 46-59 nitric oxide synthase 3 Rattus norvegicus 215-219 15123766-7 2004 In organ chamber experiments, ex vivo incubation (18 h) of rat aortic rings with the organic subfraction of ALE enhanced the NO-mediated vasodilator response to acetylcholine, indicating that the up-regulated eNOS remained functional. Acetylcholine 161-174 nitric oxide synthase 3 Rattus norvegicus 209-213 16019034-12 2005 L-NAME, an inhibitor of eNOS, almost completely abolished the dilator responses to acetylcholine, but not to sodium nitroprusside. Acetylcholine 83-96 nitric oxide synthase 3 Rattus norvegicus 24-28 15993337-7 2005 Furthermore, we observed a decreased sensitivity of diabetic vessels to acetylcholine and nitroglycerin and a decrease in both acetylcholine-stimulated NO production and phosphorylation of VASP, despite an increase in endothelial NO synthase (NOSIII) expression. Acetylcholine 127-140 nitric oxide synthase 3 Rattus norvegicus 243-249 11697135-7 2001 The impaired effect of Ach was associated with a significant reduction in eNOS activity (control: 7.7 +/- 0.8, BSO: 3.9 +/- 0.4 pmol/min/mg protein (p < .05), n = 6). Acetylcholine 23-26 nitric oxide synthase 3 Rattus norvegicus 74-78 11786479-8 2002 However, when endothelial nitric oxide synthase (eNOS) was blocked with N(G)-nitro-L-arginine, the sensitivity and maximum relaxant response to ACh was significantly higher in intact females compared with males and ovariectomized females. Acetylcholine 144-147 nitric oxide synthase 3 Rattus norvegicus 14-47 11786479-8 2002 However, when endothelial nitric oxide synthase (eNOS) was blocked with N(G)-nitro-L-arginine, the sensitivity and maximum relaxant response to ACh was significantly higher in intact females compared with males and ovariectomized females. Acetylcholine 144-147 nitric oxide synthase 3 Rattus norvegicus 49-53 12237175-12 2002 The mechanism involved in altered response to ACh in female offspring might be a consequence of reduction in estrogen levels leading to reduced eNOS activity. Acetylcholine 46-49 nitric oxide synthase 3 Rattus norvegicus 144-148 10865936-7 2000 Acetylcholine (Ach)-induced vasodilation was significantly reduced in the CuD group both before and after pretreatment with the eNOS substrate L-arginine. Acetylcholine 0-13 nitric oxide synthase 3 Rattus norvegicus 128-132 11487725-2 2001 The authors endeavored, therefore, to establish a link between these correlative findings by independently manipulating, in ovariectomized female rats, eNOS and caveolin-1 expression, while monitoring agonist (acetylcholine)-stimulated eNOS functional activity. Acetylcholine 210-223 nitric oxide synthase 3 Rattus norvegicus 236-240 11180991-12 2001 The remaining Ach-induced non-NOS vasodilation (after inhibition of NOS-1 + NOS-2 + NOS-3) was reduced from 45% in normals to 30% in septic animals. Acetylcholine 14-17 nitric oxide synthase 3 Rattus norvegicus 84-89 10996009-12 2000 After overexpression of eNOS in the atherosclerotic arteries, the response to acetylcholine was augmented, showing similar relaxation with normal vessels. Acetylcholine 78-91 nitric oxide synthase 3 Rattus norvegicus 24-28 10865936-7 2000 Acetylcholine (Ach)-induced vasodilation was significantly reduced in the CuD group both before and after pretreatment with the eNOS substrate L-arginine. Acetylcholine 15-18 nitric oxide synthase 3 Rattus norvegicus 128-132 34543641-7 2021 Though testosterone and acetylcholine (ACh) both phosphorylate Akt and eNOS, castration did not affect testosterone-mediated IPA and penile Akt or eNOS phosphorylation. Acetylcholine 24-37 nitric oxide synthase 3 Rattus norvegicus 71-75 10371698-8 1999 Acetylcholine (ACh)-stimulated vasorelaxation was decreased (P<0.05) by 30% after MI and was restored to normal with eNOS transfection. Acetylcholine 0-13 nitric oxide synthase 3 Rattus norvegicus 120-124 10371698-8 1999 Acetylcholine (ACh)-stimulated vasorelaxation was decreased (P<0.05) by 30% after MI and was restored to normal with eNOS transfection. Acetylcholine 15-18 nitric oxide synthase 3 Rattus norvegicus 120-124 10435050-6 1999 This stimulatory effect of VEGF165 on eNOS expression was associated with an elevation in the basal production of cGMP in HUVEC, and with a leftward shift of the concentration-relaxation curve to acetylcholine in aortic rings. Acetylcholine 196-209 nitric oxide synthase 3 Rattus norvegicus 38-42 34783347-7 2021 TA exhibited increased ACh-induced NO production due to a higher coupling of endothelial and neuronal NO synthases isoforms (eNOS, nNOS), and enhanced expression of antioxidant enzymes. Acetylcholine 23-26 nitric oxide synthase 3 Rattus norvegicus 125-129 34543641-7 2021 Though testosterone and acetylcholine (ACh) both phosphorylate Akt and eNOS, castration did not affect testosterone-mediated IPA and penile Akt or eNOS phosphorylation. Acetylcholine 39-42 nitric oxide synthase 3 Rattus norvegicus 71-75 34543641-8 2021 Surprisingly, castration increases ACh-mediated Akt and eNOS phosphorylation but reduces the eNOS dimer to monomer ratio. Acetylcholine 35-38 nitric oxide synthase 3 Rattus norvegicus 56-60 35602582-7 2022 Results: Highly diluted antibodies to eNOS enhanced acetylcholine-induced relaxation in a statistically significant manner. Acetylcholine 52-65 nitric oxide synthase 3 Rattus norvegicus 38-42