PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 7619088-4 1995 The drug inhibited activation of the enzyme in thrombin-stimulated platelets but not in platelets stimulated with phorbol esters, indicating that its site of action might be upstream of protein kinase C. It also inhibited the activity of phospholipase C, determined from the extent of generation of inositol phosphates and phosphatidic acid, in platelets stimulated with thrombin as well as the non-hydrolysable GTP analogue guanosine 5"-[beta, gamma-imido]triphosphate in a dose-dependent manner. Guanosine Triphosphate 412-415 coagulation factor II, thrombin Homo sapiens 47-55 18495810-3 2008 Thrombin (or other PAR1 peptide agonists), LPA, and carbachol triggered quantitatively similar Ca(2+) mobilization responses, but only thrombin and LPA caused rapid accumulation of active GTP-bound Rho. Guanosine Triphosphate 188-191 coagulation factor II, thrombin Homo sapiens 0-8 18495810-3 2008 Thrombin (or other PAR1 peptide agonists), LPA, and carbachol triggered quantitatively similar Ca(2+) mobilization responses, but only thrombin and LPA caused rapid accumulation of active GTP-bound Rho. Guanosine Triphosphate 188-191 coagulation factor II, thrombin Homo sapiens 135-143 15640525-6 2005 Thrombin also rapidly stimulated GTP loading of RhoA, which was blocked by acute pretreatment with either atorvastatin or GGTI-286. Guanosine Triphosphate 33-36 coagulation factor II, thrombin Homo sapiens 0-8 15640525-11 2005 We provide the evidence that, in thrombin-stimulated ECs, the unprocessed form of RhoA is rapidly geranylgeranylated to become the mature form, which then is converted into GTP-bound active RhoA. Guanosine Triphosphate 173-176 coagulation factor II, thrombin Homo sapiens 33-41 15130921-2 2004 Recently, we have shown that the small GTP-binding protein Ral is involved in thrombin-induced exocytosis of WPBs. Guanosine Triphosphate 39-42 coagulation factor II, thrombin Homo sapiens 78-86 12045195-3 2002 In CCL39 fibroblasts expressing either human SSTR1 or SSTR2, we demonstrate that activation of SSTR1 (but not SSTR2) attenuated both thrombin- and integrin-stimulated Rho-GTP complex formation. Guanosine Triphosphate 171-174 coagulation factor II, thrombin Homo sapiens 133-141 12045195-7 2002 To show that native SSTR1 shares the ability to inhibit Rho activation, we demonstrated that somatostatin treatment of human umbilical vein endothelial cells attenuated thrombin-stimulated Rho-GTP accumulation. Guanosine Triphosphate 193-196 coagulation factor II, thrombin Homo sapiens 169-177 10572250-1 1999 The interaction of the low-molecular-weight GTP-binding protein rap2 with the cytoskeleton from thrombin-aggregated platelets was investigated by inducing depolymerization of the actin filaments, followed by in vitro-promoted repolymerization. Guanosine Triphosphate 44-47 coagulation factor II, thrombin Homo sapiens 96-104 9741823-2 1998 The thrombin receptor transducts signals through the GTP-binding protein system, the classical pathway being the Galpha q-protein. Guanosine Triphosphate 53-56 coagulation factor II, thrombin Homo sapiens 4-12 8780738-7 1996 It is also suggested that p72syk translocation induced by thrombin requires additional signal(s) other than that mediated by the recently-cloned thrombin receptor that couples with GTP-binding proteins and interacts with TRAP. Guanosine Triphosphate 181-184 coagulation factor II, thrombin Homo sapiens 58-66 8780738-7 1996 It is also suggested that p72syk translocation induced by thrombin requires additional signal(s) other than that mediated by the recently-cloned thrombin receptor that couples with GTP-binding proteins and interacts with TRAP. Guanosine Triphosphate 181-184 coagulation factor II, thrombin Homo sapiens 145-153 16299551-9 2006 Thrombin also induced the phosphorylation of ERK1/ERK2 and elevated the Rho-GTP levels in GF. Guanosine Triphosphate 76-79 coagulation factor II, thrombin Homo sapiens 0-8 15613030-2 2004 Both thrombin, that stimulates heterotrimeric G-protein-coupled receptors, and the GPVI ligand convulxin, that activates a tyrosine-kinase based signaling pathway, were able to induced the rapid and sustained binding of GTP to Rap2B. Guanosine Triphosphate 220-223 coagulation factor II, thrombin Homo sapiens 5-13 12515716-0 2003 IQGAP2 functions as a GTP-dependent effector protein in thrombin-induced platelet cytoskeletal reorganization. Guanosine Triphosphate 22-25 coagulation factor II, thrombin Homo sapiens 56-64 12515716-5 2003 Platelet activation by alpha-thrombin, but not saturating concentrations of fibrillar collagen or adenosine 5"-diphosphate, uniquely assemble an IQGAP2/arp2/3-actin cytoplasmic complex, an association regulated by guanosine triphosphate rac1 ([GTP]rac1) but not by [GTP]cdc42. Guanosine Triphosphate 214-236 coagulation factor II, thrombin Homo sapiens 29-37 12515716-5 2003 Platelet activation by alpha-thrombin, but not saturating concentrations of fibrillar collagen or adenosine 5"-diphosphate, uniquely assemble an IQGAP2/arp2/3-actin cytoplasmic complex, an association regulated by guanosine triphosphate rac1 ([GTP]rac1) but not by [GTP]cdc42. Guanosine Triphosphate 244-247 coagulation factor II, thrombin Homo sapiens 29-37 12515716-5 2003 Platelet activation by alpha-thrombin, but not saturating concentrations of fibrillar collagen or adenosine 5"-diphosphate, uniquely assemble an IQGAP2/arp2/3-actin cytoplasmic complex, an association regulated by guanosine triphosphate rac1 ([GTP]rac1) but not by [GTP]cdc42. Guanosine Triphosphate 266-269 coagulation factor II, thrombin Homo sapiens 29-37 11799111-5 2002 Here, we found that the activation of thrombin receptors endogenously expressed in HEK-293T cells leads to a remarkable increase in the levels of GTP-bound Rho within 1 min (11-fold) and a more limited but sustained activation (4-fold) thereafter, which lasts even for several hours. Guanosine Triphosphate 146-149 coagulation factor II, thrombin Homo sapiens 38-46 11397694-4 2001 Activation of endothelial cells by thrombin resulted in transient cycling of Ral from its inactive GDP-bound to its active GTP-bound state, which coincided with release of vWF. Guanosine Triphosphate 123-126 coagulation factor II, thrombin Homo sapiens 35-43 10455022-0 1999 Rab6 is phosphorylated in thrombin-activated platelets by a protein kinase C-dependent mechanism: effects on GTP/GDP binding and cellular distribution. Guanosine Triphosphate 109-112 coagulation factor II, thrombin Homo sapiens 26-34 9238049-7 1997 Binding of 35S-labeled GTP[gammaS] to platelet membranes was decreased under both basal and thrombin-stimulated states. Guanosine Triphosphate 23-26 coagulation factor II, thrombin Homo sapiens 92-100 8042983-1 1994 Platelets accumulate PtdIns(3,4,5)P3 and PtdIns(3,4)P2 in response to thrombin and thrombin-receptor-directed peptide in a GTP-dependent manner. Guanosine Triphosphate 123-126 coagulation factor II, thrombin Homo sapiens 70-78 7630424-4 1995 In human erythroleukemia cells permeabilized with digitonin, agonist activation of thrombin and neuropeptide Y receptors increased GTP[gamma S] binding by 1.8- and 1.5-fold, respectively. Guanosine Triphosphate 131-134 coagulation factor II, thrombin Homo sapiens 83-91 7529999-0 1995 Possible involvement of protein kinase C and low molecular weight GTP-binding proteins in thrombin-induced histamine secretion in human platelets. Guanosine Triphosphate 66-69 coagulation factor II, thrombin Homo sapiens 90-98 8042983-1 1994 Platelets accumulate PtdIns(3,4,5)P3 and PtdIns(3,4)P2 in response to thrombin and thrombin-receptor-directed peptide in a GTP-dependent manner. Guanosine Triphosphate 123-126 coagulation factor II, thrombin Homo sapiens 83-91 8396137-2 1993 Thrombin stimulation also activates Ras as assessed by an increase in the proportion of Ras in a GTP bound state. Guanosine Triphosphate 97-100 coagulation factor II, thrombin Homo sapiens 0-8 8280749-5 1994 Stimulation of platelets with thrombin or concanavalin A (Con A), under non-aggregating conditions, resulted in increased 24 kDa protein-bound GTP, which also contained a significant amount of rap1B. Guanosine Triphosphate 143-146 coagulation factor II, thrombin Homo sapiens 30-38 1333596-0 1992 Exogenous GTP increases cyclic GMP and inhibits thrombin-induced aggregation of washed human platelets: comparison with ATP, adenosine and guanosine. Guanosine Triphosphate 10-13 coagulation factor II, thrombin Homo sapiens 48-56 1530576-4 1992 Dense-granule secretion induced by pervanadate required protein kinase C activity; however, aggregation and alpha-granule secretion were independent of protein kinase C. In saponin-permeabilized platelets pervanadate and thrombin stimulated phospholipase C activity by GTP-independent and GTP-dependent mechanisms respectively. Guanosine Triphosphate 269-272 coagulation factor II, thrombin Homo sapiens 221-229 1530576-4 1992 Dense-granule secretion induced by pervanadate required protein kinase C activity; however, aggregation and alpha-granule secretion were independent of protein kinase C. In saponin-permeabilized platelets pervanadate and thrombin stimulated phospholipase C activity by GTP-independent and GTP-dependent mechanisms respectively. Guanosine Triphosphate 289-292 coagulation factor II, thrombin Homo sapiens 221-229 8400019-2 1993 Muscarinic, alpha 1-adrenergic, angiotensin II, endothelin-1, thrombin, adenine nucleotide and opioid peptide receptors are all linked through GTP-binding proteins to phospholipase C which hydrolyses phosphatidylinositol 4,5-bisphosphate (PIP2) in the myocardium. Guanosine Triphosphate 143-146 coagulation factor II, thrombin Homo sapiens 62-70 8356066-0 1993 Identification of small GTP-binding rab proteins in human platelets: thrombin-induced phosphorylation of rab3B, rab6, and rab8 proteins. Guanosine Triphosphate 24-27 coagulation factor II, thrombin Homo sapiens 69-77 1333596-2 1992 GTP (10 microM-1 mM) dose-dependently inhibited thrombin-induced aggregation and serotonin secretion. Guanosine Triphosphate 0-3 coagulation factor II, thrombin Homo sapiens 48-56 1333596-8 1992 The rank order of inhibition of thrombin-induced aggregation of washed human platelets was adenosine > GTP > ATP > guanosine. Guanosine Triphosphate 106-109 coagulation factor II, thrombin Homo sapiens 32-40 1333596-9 1992 In conclusion, exogenous GTP inhibits thrombin-induced aggregation and serotonin secretion of washed human platelets by increasing cyclic GMP. Guanosine Triphosphate 25-28 coagulation factor II, thrombin Homo sapiens 38-46 3134260-2 1988 While GTP[S] itself caused a stimulation of AA release in permeabilized cells, GTP[S], GDP[S], GTP, ATP and other nucleotides inhibited AA release in response to thrombin and other agonists in intact, as well as permeabilized platelets. Guanosine Triphosphate 79-82 coagulation factor II, thrombin Homo sapiens 162-170 2112463-3 1990 A similar effect is observed on addition either of 1,2-dioctanoin or of GTP in in the presence or absence of thrombin. Guanosine Triphosphate 72-75 coagulation factor II, thrombin Homo sapiens 109-117 2494171-7 1989 In the presence of thrombin, GTP gamma S caused synergistic increase in IP3 release. Guanosine Triphosphate 29-32 coagulation factor II, thrombin Homo sapiens 19-27 2494171-8 1989 The synergism between GTP gamma S and thrombin was virtually eliminated by 10 min prior exposure to PMA (200 ng/ml). Guanosine Triphosphate 22-25 coagulation factor II, thrombin Homo sapiens 38-46 3134260-2 1988 While GTP[S] itself caused a stimulation of AA release in permeabilized cells, GTP[S], GDP[S], GTP, ATP and other nucleotides inhibited AA release in response to thrombin and other agonists in intact, as well as permeabilized platelets. Guanosine Triphosphate 79-82 coagulation factor II, thrombin Homo sapiens 162-170 2822018-4 1987 PLC activation by thrombin was dependent on GTP, and was completely inhibited by a 15-fold excess of the non-hydrolysable GDP analogue guanosine 5"-[beta-thio]diphosphate (GDP[S]). Guanosine Triphosphate 44-47 coagulation factor II, thrombin Homo sapiens 18-26 2833222-8 1988 The data indicate that, similarly to the proteinase thrombin, trypsin and trypsin-like proteinases can activate GTP-hydrolysing protein(s) that exhibit high affinity for GTP in platelet membranes. Guanosine Triphosphate 112-115 coagulation factor II, thrombin Homo sapiens 52-60 2833222-8 1988 The data indicate that, similarly to the proteinase thrombin, trypsin and trypsin-like proteinases can activate GTP-hydrolysing protein(s) that exhibit high affinity for GTP in platelet membranes. Guanosine Triphosphate 170-173 coagulation factor II, thrombin Homo sapiens 52-60 2831052-11 1988 This action of thrombin was enhanced twofold by 10-100 microM GTP and much more potently by 4-40 microM GTP[gamma S]. Guanosine Triphosphate 62-65 coagulation factor II, thrombin Homo sapiens 15-23 2831052-11 1988 This action of thrombin was enhanced twofold by 10-100 microM GTP and much more potently by 4-40 microM GTP[gamma S]. Guanosine Triphosphate 104-107 coagulation factor II, thrombin Homo sapiens 15-23 2822018-11 1987 It is concluded that an early post-receptor effect of thrombin is the activation of a Ca2+- and GTP-dependent membrane-associated PLC that specifically cleaves PIP2 and PIP. Guanosine Triphosphate 96-99 coagulation factor II, thrombin Homo sapiens 54-62 2822018-12 1987 This result suggests that the cell-surface receptor for thrombin is coupled to a polyphosphoinositide-specific PLC by a GTP-binding protein that regulates PLC activity by increasing its sensitivity to Ca2+. Guanosine Triphosphate 120-123 coagulation factor II, thrombin Homo sapiens 56-64 3106084-1 1987 GTP-dependent release of Ca2+ by thrombin, and inhibition by pertussis toxin and a monoclonal antibody that blocks calcium release by IP3. Guanosine Triphosphate 0-3 coagulation factor II, thrombin Homo sapiens 33-41 3107563-2 1987 Ca2+ has opposite effects on the formation of [3H]inositol phosphates induced by thrombin or GTP gamma S. While the action of GTP gamma S on the formation of [3H]inositol phosphates is inhibited by Ca2+, action of thrombin is stimulated by Ca2+. Guanosine Triphosphate 126-129 coagulation factor II, thrombin Homo sapiens 81-89 3107563-2 1987 Ca2+ has opposite effects on the formation of [3H]inositol phosphates induced by thrombin or GTP gamma S. While the action of GTP gamma S on the formation of [3H]inositol phosphates is inhibited by Ca2+, action of thrombin is stimulated by Ca2+. Guanosine Triphosphate 126-129 coagulation factor II, thrombin Homo sapiens 214-222 3107563-3 1987 Guanosine 5"-O-(2-thiodiphosphate) (GDP beta S), which inhibits the function of GTP-binding proteins, also inhibits the effect of GTP gamma S on phospholipase C stimulation but, surprisingly, increases the effect of thrombin. Guanosine Triphosphate 80-83 coagulation factor II, thrombin Homo sapiens 216-224 3107563-3 1987 Guanosine 5"-O-(2-thiodiphosphate) (GDP beta S), which inhibits the function of GTP-binding proteins, also inhibits the effect of GTP gamma S on phospholipase C stimulation but, surprisingly, increases the effect of thrombin. Guanosine Triphosphate 130-133 coagulation factor II, thrombin Homo sapiens 216-224 3107563-6 1987 However, the effect of thrombin may be modulated by the action of an inhibitory GTP-binding protein. Guanosine Triphosphate 80-83 coagulation factor II, thrombin Homo sapiens 23-31 3106084-4 1987 This effect of thrombin/GTP was blocked by a monoclonal antibody that binds to vesicles and prevents Ca2+ release by IP3. Guanosine Triphosphate 24-27 coagulation factor II, thrombin Homo sapiens 15-23 3112131-2 1987 GTP or GTP gamma S also enhanced thrombin-induced [3H]arachidonic acid release in permeabilized platelets. Guanosine Triphosphate 0-3 coagulation factor II, thrombin Homo sapiens 33-41 3023367-5 1986 In platelets permeabilized with saponin, nonhydrolyzable GTP analogs reproduced the effects of thrombin by causing diacylglycerol formation, Ca2+ release from the dense tubular system and serotonin secretion. Guanosine Triphosphate 57-60 coagulation factor II, thrombin Homo sapiens 95-103 3026330-7 1986 The data suggest that thrombin interaction with its receptor sites in platelet membranes leads to stimulation of two GTP-hydrolysing enzymes. Guanosine Triphosphate 117-120 coagulation factor II, thrombin Homo sapiens 22-30 3021450-6 1986 GTP markedly enhances the effect of thrombin on Ca2+-dependent 5-hydroxytryptamine secretion by increasing the maximal extent of the response and decreasing the thrombin concentration required to give half-maximal response. Guanosine Triphosphate 0-3 coagulation factor II, thrombin Homo sapiens 36-44 3021450-6 1986 GTP markedly enhances the effect of thrombin on Ca2+-dependent 5-hydroxytryptamine secretion by increasing the maximal extent of the response and decreasing the thrombin concentration required to give half-maximal response. Guanosine Triphosphate 0-3 coagulation factor II, thrombin Homo sapiens 161-169 3026330-9 1986 The other GTP-hydrolysing enzyme activated by thrombin may represent a guanine nucleotide-binding protein apparently involved in the coupling of thrombin receptors to the phosphoinositide phosphodiesterase. Guanosine Triphosphate 10-13 coagulation factor II, thrombin Homo sapiens 46-54 3026330-9 1986 The other GTP-hydrolysing enzyme activated by thrombin may represent a guanine nucleotide-binding protein apparently involved in the coupling of thrombin receptors to the phosphoinositide phosphodiesterase. Guanosine Triphosphate 10-13 coagulation factor II, thrombin Homo sapiens 145-153 3015130-4 1986 Maximal activation of thrombin-induced phosphoinositide hydrolysis was observed at 10 uM GTP. Guanosine Triphosphate 89-92 coagulation factor II, thrombin Homo sapiens 22-30 6098673-6 1984 Moreover, GTP potentiated the stimulatory action of thrombin. Guanosine Triphosphate 10-13 coagulation factor II, thrombin Homo sapiens 52-60 3026330-1 1986 Thrombin inhibits adenylate cyclase and stimulates GTP hydrolysis by high-affinity GTPase(s) in membranes of human platelets at almost identical concentrations. Guanosine Triphosphate 51-54 coagulation factor II, thrombin Homo sapiens 0-8 3026330-3 1986 However, stimulation of GTP hydrolysis caused by adrenaline (alpha 2-adrenoceptor agonist) and by thrombin at maximally effective concentrations was partially additive, whereas with regard to adenylate cyclase inhibition no additive response was observed. Guanosine Triphosphate 24-27 coagulation factor II, thrombin Homo sapiens 98-106 3026330-5 1986 Additionally, the thiol reagent N-ethylmalemide (NEM) at rather low concentrations abolished thrombin- and adrenaline-induced stimulation of GTP hydrolysis was decreased by only 30-40% by treatment of platelet membranes with even high concentrations of NEM. Guanosine Triphosphate 141-144 coagulation factor II, thrombin Homo sapiens 93-101 3032044-4 1986 In both types of reactions, thrombin binding and/or proteolysis is linked to changes in GTP-binding proteins, protein kinase C, or other pathways. Guanosine Triphosphate 88-91 coagulation factor II, thrombin Homo sapiens 28-36 6437813-3 1984 Up to a concentration of about 1 U/ml, thrombin inhibited the enzyme in a strictly GTP-dependent manner by maximal 60-70%, with half-maximal inhibition occurring at about 0.005 U/ml thrombin. Guanosine Triphosphate 83-86 coagulation factor II, thrombin Homo sapiens 39-47 6437813-3 1984 Up to a concentration of about 1 U/ml, thrombin inhibited the enzyme in a strictly GTP-dependent manner by maximal 60-70%, with half-maximal inhibition occurring at about 0.005 U/ml thrombin. Guanosine Triphosphate 83-86 coagulation factor II, thrombin Homo sapiens 182-190 6437813-4 1984 At higher concentrations, thrombin-induced inhibition of platelet adenylate cyclase was independent of GTP. Guanosine Triphosphate 103-106 coagulation factor II, thrombin Homo sapiens 26-34 6437813-7 1984 Additionally, thrombin accelerated and potentiated the enzyme inhibition caused by the stable GTP analog, guanosine 5"-(gamma-thio)triphosphate. Guanosine Triphosphate 94-97 coagulation factor II, thrombin Homo sapiens 14-22 6437813-8 1984 Furthermore, at identical concentrations causing adenylate cyclase inhibition, thrombin effectively stimulated GTP hydrolysis in platelet membranes. Guanosine Triphosphate 111-114 coagulation factor II, thrombin Homo sapiens 79-87 29735342-9 2018 The interaction of thrombin with protease activated receptor-1 activates the Rho A guanosine triphosphate (GTP)ase-myosin light chain (MLC) phosphorylation signaling axis, leading to actin cytoskeleton changes. Guanosine Triphosphate 83-105 coagulation factor II, thrombin Homo sapiens 19-27 29735342-9 2018 The interaction of thrombin with protease activated receptor-1 activates the Rho A guanosine triphosphate (GTP)ase-myosin light chain (MLC) phosphorylation signaling axis, leading to actin cytoskeleton changes. Guanosine Triphosphate 107-110 coagulation factor II, thrombin Homo sapiens 19-27 28509344-10 2017 Thrombin caused the GTP loading and activation of RhoA, leading to the ROCK-mediated phosphorylation of MLCP on threonine 853 (thr853 ), which is known to inhibit phosphatase activity. Guanosine Triphosphate 20-23 coagulation factor II, thrombin Homo sapiens 0-8