PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 18598255-1 2008 The monomeric GTP-binding protein p21Ras has been repeatedly implicated in neuronal stability and plastic changes of the adult nervous system. Guanosine Triphosphate 14-17 Harvey rat sarcoma virus oncogene Mus musculus 34-40 9720766-0 1998 Ligation of the alpha2M signalling receptor elevates the levels of p21Ras-GTP in macrophages. Guanosine Triphosphate 74-77 Harvey rat sarcoma virus oncogene Mus musculus 67-73 16306046-8 2006 That the mechanical repression of RANKL requires activation of H-Ras, a specific isoform of Ras-GTP that is known to reside in the lipid raft microdomain, suggests that spatial arrangements are critical for generation of specific downstream events in response to mechanical signals. Guanosine Triphosphate 96-99 Harvey rat sarcoma virus oncogene Mus musculus 63-68 12642594-1 2003 H-Ras displays dynamic cycles of GTP binding and palmitate turnover. Guanosine Triphosphate 33-36 Harvey rat sarcoma virus oncogene Mus musculus 0-5 12642594-5 2003 Combining this data with GTP binding measurements, the palmitate half-life of H-Ras in the fully GTP-bound state was estimated to be less than 10 min. Guanosine Triphosphate 25-28 Harvey rat sarcoma virus oncogene Mus musculus 78-83 12642594-5 2003 Combining this data with GTP binding measurements, the palmitate half-life of H-Ras in the fully GTP-bound state was estimated to be less than 10 min. Guanosine Triphosphate 97-100 Harvey rat sarcoma virus oncogene Mus musculus 78-83 10844550-5 2000 All papillomas analyzed (six of six) had mutations in codon 61 of H-ras, demonstrating strong cooperation between the Nf1 GTPase activating protein for Ras, neurofibromin, and Ras-GTP. Guanosine Triphosphate 122-125 Harvey rat sarcoma virus oncogene Mus musculus 66-71 10433077-1 1999 We previously reported that a decreased TCR mediated activity of the GTP-GDP binding p21ras protooncogene is associated with prediabetes in non-obese diabetic (NOD) mice. Guanosine Triphosphate 69-72 Harvey rat sarcoma virus oncogene Mus musculus 85-91 9888515-2 1998 We also found that stimulation of NG108-15 cells with Ang II induced a rapid decrease in GTP-bound p21ras. Guanosine Triphosphate 89-92 Harvey rat sarcoma virus oncogene Mus musculus 99-105 16908535-9 2006 Additionally, Kras2-mutant tumors exhibited substantially higher levels of ras-GTP, phospho-Erk1/2, and phospho-Mek1/2 compared to Hras1-mutant tumors, suggesting the involvement of the ras/mitogen-activated protein kinase (MAPK) pathway in the acquisition of oncogene independence. Guanosine Triphosphate 79-82 Harvey rat sarcoma virus oncogene Mus musculus 14-19 16710563-8 2006 Third, c-Ki-Ras-GTP high basal levels suppress activation of the c-H-Ras onco-protein. Guanosine Triphosphate 16-19 Harvey rat sarcoma virus oncogene Mus musculus 65-72 16710563-9 2006 But, Y1 cells, expressing dominant negative mutant RasN17, display a rapid and transient up-regulation of c-H-Ras-GTP upon FGF2 treatment. Guanosine Triphosphate 114-117 Harvey rat sarcoma virus oncogene Mus musculus 106-113 16117800-6 2005 Total H-ras protein and cyclin D1 were found to increase during DB[a,l]P-induced hyperplasia, but the levels of guanosine triphosphate-bound (active) H-ras protein and cyclin E were increased during the putative clonal proliferation of codon 61-mutated cells. Guanosine Triphosphate 112-134 Harvey rat sarcoma virus oncogene Mus musculus 150-155 11180512-0 2001 Interferon-gamma protects astrocytes from apoptosis and increases the formation of p21ras-GTP complex through ras oncogene family overexpression. Guanosine Triphosphate 90-93 Harvey rat sarcoma virus oncogene Mus musculus 83-89 10801823-7 2000 SNC also decreased Ha-Ras GTP binding and inhibited phosphorylation of the kinases ERK1 and ERK2 in NIH 3T3 cells. Guanosine Triphosphate 26-29 Harvey rat sarcoma virus oncogene Mus musculus 19-25 10478850-4 1999 Stimulation of NG108-15 cells with Ang II induced a decrease in GTP-bound p21ras but a sustained increase in the activity of p42mapk and p44mapk as well as neurite outgrowth. Guanosine Triphosphate 64-67 Harvey rat sarcoma virus oncogene Mus musculus 74-80 9856997-5 1998 Our results show that not only the intrinsic GTP/GTP exchange on Ha-Ras but also the GEF-stimulated exchange is inhibited in a concentration-dependent manner by the RBDs of Raf. Guanosine Triphosphate 45-48 Harvey rat sarcoma virus oncogene Mus musculus 65-71 9856997-5 1998 Our results show that not only the intrinsic GTP/GTP exchange on Ha-Ras but also the GEF-stimulated exchange is inhibited in a concentration-dependent manner by the RBDs of Raf. Guanosine Triphosphate 49-52 Harvey rat sarcoma virus oncogene Mus musculus 65-71 9856997-6 1998 Conversely, the scintillation proximity assay, which monitors the effect of GEF on the Ras.Raf complex, showed that the binding of Raf and GEF to Ha-Ras.GTP is mutually exclusive. Guanosine Triphosphate 153-156 Harvey rat sarcoma virus oncogene Mus musculus 146-152 9856997-8 1998 It is noteworthy that under more physiological conditions mimicking the cellular GDP/GTP ratio, Raf enhances the GEF-stimulated GDP/GTP exchange on Ha-Ras, in agreement with the sequestration of Ras.GTP by Raf. Guanosine Triphosphate 85-88 Harvey rat sarcoma virus oncogene Mus musculus 148-154 9856997-8 1998 It is noteworthy that under more physiological conditions mimicking the cellular GDP/GTP ratio, Raf enhances the GEF-stimulated GDP/GTP exchange on Ha-Ras, in agreement with the sequestration of Ras.GTP by Raf. Guanosine Triphosphate 132-135 Harvey rat sarcoma virus oncogene Mus musculus 148-154 9856997-8 1998 It is noteworthy that under more physiological conditions mimicking the cellular GDP/GTP ratio, Raf enhances the GEF-stimulated GDP/GTP exchange on Ha-Ras, in agreement with the sequestration of Ras.GTP by Raf. Guanosine Triphosphate 132-135 Harvey rat sarcoma virus oncogene Mus musculus 148-154 9856997-9 1998 Consistent with our results, the GEF-stimulated exchange of Ha-Ras.GTP was also inhibited by another effector of Ras, the RBD (amino acid residues 133-314) of phosphatidylinositol 3-kinase p110alpha. Guanosine Triphosphate 67-70 Harvey rat sarcoma virus oncogene Mus musculus 60-66 9720766-3 1998 Both alpha2M-methylamine (50 pM) and RBF (50 pM) stimulated a 2-3-fold increase in the formation of the p21Ras-GTP complex compared with unstimulated cells. Guanosine Triphosphate 111-114 Harvey rat sarcoma virus oncogene Mus musculus 104-110 9374526-10 1997 Of several prenylated GTP-binding proteins tested, including Ki-Ras4B, Ha-Ras, RhoB, RhoA, and Rap1B, only Ki-Ras was found to bind significantly to microtubules, and this was in a prenylation-dependent fashion. Guanosine Triphosphate 22-25 Harvey rat sarcoma virus oncogene Mus musculus 71-77 8950978-3 1996 The contribution of v-Ha-ras to the overall p21ras-GTP content was evaluated by metabolic labelling with 32P. Guanosine Triphosphate 51-54 Harvey rat sarcoma virus oncogene Mus musculus 44-50 9349502-1 1997 R-Ras belongs to a family of low molecular weight GTP-binding proteins and exhibits 55% amino acid identity to H-Ras. Guanosine Triphosphate 50-53 Harvey rat sarcoma virus oncogene Mus musculus 111-116 8950978-4 1996 Surprisingly, p21ras-GTP complexes were predominant in the serum-deprived BP-A31 cells as well as in the Ras2 cells. Guanosine Triphosphate 21-24 Harvey rat sarcoma virus oncogene Mus musculus 14-20 8950978-5 1996 The excess of p21ras-GTP was not due to the lack of the GTPase activating protein. Guanosine Triphosphate 21-24 Harvey rat sarcoma virus oncogene Mus musculus 14-20 7721808-0 1995 Prostaglandin F2 alpha stimulates formation of p21ras-GTP complex and mitogen-activated protein kinase in NIH-3T3 cells via Gq-protein-coupled pathway. Guanosine Triphosphate 54-57 Harvey rat sarcoma virus oncogene Mus musculus 47-53 8621713-4 1996 Antibodies against p21ras, Galphaq, -beta, or -gamma2 subunits of the GTP-binding protein inhibit MAP kinase-dependent phosphorylation. Guanosine Triphosphate 70-73 Harvey rat sarcoma virus oncogene Mus musculus 19-25 8575075-1 1996 Transformation of fibroblast-like cells (NIH 3T3) by a constitutively activated GTP-bound isoform of p21ras (EJ-Ras) produces morphogenic changes characterized by decreased attachment to the substratum, with retraction and rounding of the cell body. Guanosine Triphosphate 80-83 Harvey rat sarcoma virus oncogene Mus musculus 101-107 8782662-11 1996 Furthermore, NB-506 decreased the amount of the GTP-bound form of the H-ras product in pT22-3 cells. Guanosine Triphosphate 48-51 Harvey rat sarcoma virus oncogene Mus musculus 70-75 7691239-5 1993 Inhibition of p210 bcr-abl-dependent tyrosine phosphorylation resulted in a reduction of active p21RAS-GTP complexes in the transformed cells, in diminished expression of the nuclear early response genes c-jun and c-fos, and in lower cellular proliferation rate. Guanosine Triphosphate 103-106 Harvey rat sarcoma virus oncogene Mus musculus 96-102 7662118-0 1995 Ha-ras p21-GTP levels remain constant during primary keratinocyte differentiation. Guanosine Triphosphate 11-14 Harvey rat sarcoma virus oncogene Mus musculus 0-6 7662118-7 1995 The percentage of Ha-ras p21 protein in its active, GTP-bound form also remained unchanged during primary adult keratinocyte differentiation and in immortalized, benign, and malignant keratinocytes subjected to differentiating conditions. Guanosine Triphosphate 52-55 Harvey rat sarcoma virus oncogene Mus musculus 18-24 7829473-1 1995 Insulin receptor signaling acutely stimulates GTP loading of p21ras, apparently by mobilizing complexes of Grb2 and the guanine nucleotide exchangers Son-of-sevenless (Sos) 1 and 2 to associate with tyrosine-phosphorylated proteins in the plasma membrane. Guanosine Triphosphate 46-49 Harvey rat sarcoma virus oncogene Mus musculus 61-67 7829473-2 1995 Here we show that in 32P-labeled 3T3-L1 adipocytes the elevated cellular concentrations of [32P]GTP-bound p21ras in response to insulin return to near basal levels after 20-30 min of hormone stimulation, while insulin receptors remain activated. Guanosine Triphosphate 96-99 Harvey rat sarcoma virus oncogene Mus musculus 106-112 7523375-5 1994 Steroid treatment (dexamethasone, 1 microM) specifically induced c-Ha-ras(Asn-17) protein and mRNA and blocked IL-3-induced accumulation of p21ras-GTP in 32Dcl3/p21rasN17 cell lines, but not in control cells. Guanosine Triphosphate 147-150 Harvey rat sarcoma virus oncogene Mus musculus 140-146 8228821-4 1993 First, stimulation of TA3 7.9 Ag-specific murine B lymphoma cells for 2 min with either Ag or F(ab")2 anti-IgM induces p21ras activation as measured by an increase in the GTP/GDP ratio of its bound nucleotides. Guanosine Triphosphate 171-174 Harvey rat sarcoma virus oncogene Mus musculus 119-125 8378348-0 1993 GTP-dependent association of Raf-1 with Ha-Ras: identification of Raf as a target downstream of Ras in mammalian cells. Guanosine Triphosphate 0-3 Harvey rat sarcoma virus oncogene Mus musculus 40-46 8378348-4 1993 In an effort to identify the Ras-associated downstream proteins, we added recombinant Ha-Ras in a GTP-bound form to cell-free lysates and used several antibodies against Ras to immunoprecipitate Ras complexes. Guanosine Triphosphate 98-101 Harvey rat sarcoma virus oncogene Mus musculus 86-92 8378348-7 1993 The GTP-dependent association of Ha-Ras with Raf-1 was observed with lysates of various types of cultured cells, including NIH 3T3, pheochromocytoma (PC) 12, Ba/F3, and Jurkat T cells, and also with crude extracts from rat brain. Guanosine Triphosphate 4-7 Harvey rat sarcoma virus oncogene Mus musculus 33-39 8354267-5 1993 The small GTP-binding protein Ha-ras was reduced by 50%, whereas rab1 and other small GTP-binding proteins tentatively identified as rab-isoforms (ras-homologous gene products from brain) were increased by 100% and 70%, respectively. Guanosine Triphosphate 10-13 Harvey rat sarcoma virus oncogene Mus musculus 30-36 8455619-1 1993 Although p21ras is localized to the plasma membrane, proteins it interacts with, such as the GTPase-activating proteins (GAPs) ras GAP and neurofibromin (NF1), are not, suggesting that one function of p21ras GTP may be to target such proteins to the plasma membrane. Guanosine Triphosphate 93-96 Harvey rat sarcoma virus oncogene Mus musculus 9-15 2549064-1 1989 Mutational replacements of specific residues in the GTP-binding pocket of the 21-kDa ras proteins (p21ras) reduce their GTPase activity. Guanosine Triphosphate 52-55 Harvey rat sarcoma virus oncogene Mus musculus 99-105 7681161-0 1993 Increased levels of p21ras-GTP and enhanced DNA synthesis accompany elevated tyrosyl phosphorylation of GAP-associated proteins, p190 and p62, in c-src overexpressors. Guanosine Triphosphate 27-30 Harvey rat sarcoma virus oncogene Mus musculus 20-26 7681161-20 1993 However, analysis of GTP-bound p21ras revealed higher basal and EGF-stimulated levels in c-src overexpressors than in control cells. Guanosine Triphosphate 21-24 Harvey rat sarcoma virus oncogene Mus musculus 31-37 1508192-5 1992 Furthermore, PDGF exposure led to a rapid and sustained increase in the levels of p21ras bound to GTP, with kinetics similar to those observed for GAP tyrosine phosphorylation. Guanosine Triphosphate 98-101 Harvey rat sarcoma virus oncogene Mus musculus 82-88 1508192-6 1992 The PDGF-induced increases in GTP-bound p21ras in NIH 3T3 cells were comparable to the steady-state level observed in serum-starved c-H-ras-overexpressing transformants, conditions in which these cells maintained high rates of DNA synthesis. Guanosine Triphosphate 30-33 Harvey rat sarcoma virus oncogene Mus musculus 40-46 1508192-8 1992 Addition of PDGF to c-H-ras-overexpressing cells also resulted in a rapid and sustained increase in GTP-bound p21ras. Guanosine Triphosphate 100-103 Harvey rat sarcoma virus oncogene Mus musculus 20-27 1508192-8 1992 Addition of PDGF to c-H-ras-overexpressing cells also resulted in a rapid and sustained increase in GTP-bound p21ras. Guanosine Triphosphate 100-103 Harvey rat sarcoma virus oncogene Mus musculus 110-116 2017178-3 1991 A GTPase-activating protein (GAP) mediates the inactivation of p21ras by facilitating the conversion of the active p21ras-GTP to the inactive p21ras-GDP. Guanosine Triphosphate 2-5 Harvey rat sarcoma virus oncogene Mus musculus 63-69 2017178-3 1991 A GTPase-activating protein (GAP) mediates the inactivation of p21ras by facilitating the conversion of the active p21ras-GTP to the inactive p21ras-GDP. Guanosine Triphosphate 2-5 Harvey rat sarcoma virus oncogene Mus musculus 115-121 1425589-1 1992 It has been shown previously in T cells that stimulation of protein kinase C or the T cell antigen receptor leads to a rapid and persistent activation of p21ras as measured by a dramatic increase in the amount of bound GTP. Guanosine Triphosphate 219-222 Harvey rat sarcoma virus oncogene Mus musculus 154-160 1404241-7 1992 TCDD evoked increases in hepatic p21ras protein of C57BL/6J mice were accompanied by the increase in the specific binding of GTP to hepatic plasma membranes. Guanosine Triphosphate 125-128 Harvey rat sarcoma virus oncogene Mus musculus 33-39 2017178-2 1991 The activity of p21ras itself is regulated by its cyclic association with GDP-GTP, where p21ras-GTP is the active form and p21ras-GDP is the inactive form. Guanosine Triphosphate 78-81 Harvey rat sarcoma virus oncogene Mus musculus 16-22 2017178-2 1991 The activity of p21ras itself is regulated by its cyclic association with GDP-GTP, where p21ras-GTP is the active form and p21ras-GDP is the inactive form. Guanosine Triphosphate 78-81 Harvey rat sarcoma virus oncogene Mus musculus 89-95 2017178-2 1991 The activity of p21ras itself is regulated by its cyclic association with GDP-GTP, where p21ras-GTP is the active form and p21ras-GDP is the inactive form. Guanosine Triphosphate 78-81 Harvey rat sarcoma virus oncogene Mus musculus 89-95 2198577-0 1990 Platelet-derived growth factor stimulates formation of active p21ras.GTP complex in Swiss mouse 3T3 cells. Guanosine Triphosphate 69-72 Harvey rat sarcoma virus oncogene Mus musculus 62-68 35069209-1 2021 HRas-GTP has a transient intermediate state with a "non-signaling open conformation" in GTP hydrolysis and nucleotide exchange. Guanosine Triphosphate 5-8 Harvey rat sarcoma virus oncogene Mus musculus 0-4 35069209-1 2021 HRas-GTP has a transient intermediate state with a "non-signaling open conformation" in GTP hydrolysis and nucleotide exchange. Guanosine Triphosphate 88-91 Harvey rat sarcoma virus oncogene Mus musculus 0-4 2549064-4 1989 alpha s in which leucine replaces glutamine 227 (corresponding to glutamine 61 of p21ras) constitutively activates adenylyl cyclase and reduces the kcat for GTP hydrolysis more than 100-fold. Guanosine Triphosphate 157-160 Harvey rat sarcoma virus oncogene Mus musculus 82-88 2549064-8 1989 We conclude that alpha s residues near glutamine 227 and glycine 49 participate in binding and hydrolysis of GTP, although the GTP binding regions of alpha s and p21ras are not identical. Guanosine Triphosphate 109-112 Harvey rat sarcoma virus oncogene Mus musculus 162-168 2549064-8 1989 We conclude that alpha s residues near glutamine 227 and glycine 49 participate in binding and hydrolysis of GTP, although the GTP binding regions of alpha s and p21ras are not identical. Guanosine Triphosphate 127-130 Harvey rat sarcoma virus oncogene Mus musculus 162-168 3380784-4 1988 The most noticeable change was observed in the increased activity of specific protein-tyrosine kinases and protein kinase C and an increased level of p21ras-associated binding of [3H]GTP. Guanosine Triphosphate 183-186 Harvey rat sarcoma virus oncogene Mus musculus 150-156 3141783-1 1988 The postmicrosomal fraction of the extract from NIH 3T3 and BALB/c 3T3 cells stimulated the hydrolysis of GTP bound to H-ras gene product p21 by severalfold. Guanosine Triphosphate 106-109 Harvey rat sarcoma virus oncogene Mus musculus 119-124 3092218-7 1986 Sequence homologies with p21ras and elongation factor Tu identify regions of the alpha chains that form the site for GTP binding and hydrolysis. Guanosine Triphosphate 117-120 Harvey rat sarcoma virus oncogene Mus musculus 25-31 3011420-0 1986 Deletion mutants of Harvey ras p21 protein reveal the absolute requirement of at least two distant regions for GTP-binding and transforming activities. Guanosine Triphosphate 111-114 Harvey rat sarcoma virus oncogene Mus musculus 20-30 21189290-6 2011 Moreover, H-Ras formed a complex with Cdc42 on the endomembrane, and this interaction was enhanced when H-Ras was GTP bound or when cells were stimulated by growth factors. Guanosine Triphosphate 114-117 Harvey rat sarcoma virus oncogene Mus musculus 10-15 23136424-2 2012 Here, we report that the GTP binding protein, H-Ras in the nucleus accumbens (NAc) plays a key role in neuroadaptations that underlie excessive alcohol-drinking behaviors. Guanosine Triphosphate 25-28 Harvey rat sarcoma virus oncogene Mus musculus 46-51 21501659-4 2011 The protein product of the Nf1 gene is neurofibromin, a guanosine triphosphatase-activating protein (GAP) for p21ras (Ras) that accelerates the conversion of active Ras-GTP to inactive Ras-GDP. Guanosine Triphosphate 169-172 Harvey rat sarcoma virus oncogene Mus musculus 110-116 21189290-6 2011 Moreover, H-Ras formed a complex with Cdc42 on the endomembrane, and this interaction was enhanced when H-Ras was GTP bound or when cells were stimulated by growth factors. Guanosine Triphosphate 114-117 Harvey rat sarcoma virus oncogene Mus musculus 104-109