PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 1473149-1 1992 From the budding yeast S. cerevisiae, we have cloned a gene, YPT7, that encodes a GTP-binding protein belonging to the Ypt family of ras-related proteins. Guanosine Triphosphate 82-85 Rab family GTPase YPT7 Saccharomyces cerevisiae S288C 61-65 27385334-9 2016 Strikingly, it was only critical for Ypt7 to be GTP loaded on membranes bearing the R-SNARE Nyv1, whereas the bound nucleotide of Ypt7 was irrelevant on membranes bearing the Q-SNAREs Vam3 and Vti1. Guanosine Triphosphate 48-51 Rab family GTPase YPT7 Saccharomyces cerevisiae S288C 37-41 25995379-4 2015 When HOPS is phosphorylated by the vacuolar casein kinase I, Yck3p, tethering only takes place when GTP-bound Ypt7p is present in both tethered membranes. Guanosine Triphosphate 100-103 Rab family GTPase YPT7 Saccharomyces cerevisiae S288C 110-115 19386605-2 2009 HOPS is a multisubunit protein with direct affinities for SNAREs, vacuolar lipids, and the GTP-bound form of Ypt7p; each of these affinities contributes to HOPS association with the organelle. Guanosine Triphosphate 91-94 Rab family GTPase YPT7 Saccharomyces cerevisiae S288C 109-114 25411340-5 2015 After phosphorylation by the vacuolar kinase Yck3p, phospho-HOPS needs both Ypt7p:GTP and acidic lipids to support fusion. Guanosine Triphosphate 82-85 Rab family GTPase YPT7 Saccharomyces cerevisiae S288C 76-81 22787280-5 2012 However, the phosphorylation of HOPS by recombinant Yck3p confers a strict requirement for GTP-bound Ypt7p for binding phosphorylated HOPS, for optimal membrane tethering, and for proteoliposome fusion. Guanosine Triphosphate 91-94 Rab family GTPase YPT7 Saccharomyces cerevisiae S288C 101-106 22787280-6 2012 Added GTPase-activating protein promotes GTP hydrolysis by Ypt7p, and added GDI captures Ypt7p in its GDP-bound state during nucleotide cycling. Guanosine Triphosphate 6-9 Rab family GTPase YPT7 Saccharomyces cerevisiae S288C 59-64 22787280-7 2012 In either case, the net conversion of Ypt7:GTP to Ypt7:GDP has no effect on HOPS binding or activity but blocks fusion mediated by phosphorylated HOPS. Guanosine Triphosphate 43-46 Rab family GTPase YPT7 Saccharomyces cerevisiae S288C 38-42 22787280-7 2012 In either case, the net conversion of Ypt7:GTP to Ypt7:GDP has no effect on HOPS binding or activity but blocks fusion mediated by phosphorylated HOPS. Guanosine Triphosphate 43-46 Rab family GTPase YPT7 Saccharomyces cerevisiae S288C 50-54 21062894-4 2010 We show that overexpression of Ypt7 results in expansion and massive invagination of the vacuolar membrane, which requires cycling of Ypt7 between GDP- and GTP-bound states. Guanosine Triphosphate 156-159 Rab family GTPase YPT7 Saccharomyces cerevisiae S288C 31-35 19826089-4 2009 We now report reconstitution of lysis-free membrane fusion that requires purified GTP-bound Ypt7p, HOPS complex, vacuolar SNAREs, ATP hydrolysis, and the SNARE disassembly catalysts Sec17p and Sec18p. Guanosine Triphosphate 82-85 Rab family GTPase YPT7 Saccharomyces cerevisiae S288C 92-97 18809726-5 2008 Phosphorylation of both substrates is opposed by Ypt7-guanosine triphosphate (GTP). Guanosine Triphosphate 78-81 Rab family GTPase YPT7 Saccharomyces cerevisiae S288C 49-53 18809726-7 2008 Gyp7-stimulated GTP hydrolysis on Ypt7 therefore appears to trigger both passive termination of Ypt7 signaling and active kinase-mediated inhibition of Ypt7"s downstream targets. Guanosine Triphosphate 16-19 Rab family GTPase YPT7 Saccharomyces cerevisiae S288C 34-38 18809726-7 2008 Gyp7-stimulated GTP hydrolysis on Ypt7 therefore appears to trigger both passive termination of Ypt7 signaling and active kinase-mediated inhibition of Ypt7"s downstream targets. Guanosine Triphosphate 16-19 Rab family GTPase YPT7 Saccharomyces cerevisiae S288C 96-100 18809726-7 2008 Gyp7-stimulated GTP hydrolysis on Ypt7 therefore appears to trigger both passive termination of Ypt7 signaling and active kinase-mediated inhibition of Ypt7"s downstream targets. Guanosine Triphosphate 16-19 Rab family GTPase YPT7 Saccharomyces cerevisiae S288C 96-100 10725336-4 2000 The Vam2/6p complex then binds to Ypt7p, a guanosine triphosphate binding protein of the Rab family, to initiate productive contact between vacuoles. Guanosine Triphosphate 43-65 Rab family GTPase YPT7 Saccharomyces cerevisiae S288C 34-39