PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 7581745-3 1994 The exchange of GDP bound to chain initiation factor 2 (eIF-2) for GTP by GEF is a rate limiting step in protein synthesis. Guanosine Triphosphate 67-70 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 74-77 8756332-1 1996 Members of the Ras subfamily of small GTP-binding proteins have been shown to be promiscuous towards a variety of putative effector molecules such as the protein kinase c-Raf and the Ral-specific guanine nucleotide exchange factor (Ral-GEF). Guanosine Triphosphate 38-41 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 236-239 8589075-3 1995 The enzyme becomes active after assembly on a membrane bound flavocytochrome b of cytosolic factors p47 phox, p67 phox and p40 phox and of low molecular mass GTP binding proteins. Guanosine Triphosphate 158-161 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 123-126 8049218-11 1994 In the presence of 1 mM Mg2+, the amount of GTP bound to dephosphorylated GEF is reduced 3.8-fold as compared to that of the CK-II-phosphorylated GEF. Guanosine Triphosphate 44-47 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 146-149 8049218-12 1994 Rephosphorylation with CK-II restores GTP binding and increases 4-5-fold the activity of GEF in the exchange of eIF-2-bound GDP for free GTP. Guanosine Triphosphate 137-140 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 89-92 8049218-14 1994 The rate of GEF-catalyzed exchange of dephosphorylated eIF-2-bound GDP for GTP is approximately 2-fold slower than that with the isolated eIF-2. Guanosine Triphosphate 75-78 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 12-15 7581745-6 1994 On the other hand, phosphorylation of the largest subunit of GEF (82-kD subunit) by casein kinase (CK) I or II stimulates GDP/GTP exchange. Guanosine Triphosphate 126-129 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 61-64 3947361-1 1986 A guanine nucleotide exchange factor (GEF), catalyzing the exchange of GDP bound to initiation factor eIF-2 for GTP, has been isolated from S3 HeLa cells as the eIF-2 X GEF complex and extensively purified by procedures originally developed for purification of GEF from rabbit reticulocytes. Guanosine Triphosphate 112-115 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 38-41 2229078-15 1990 The rabbit reticulocyte guanine nucleotide exchange factor (GEF) catalyzes the exchange of GDP bound to sea urchin eIF-2 for GTP and stimulates ternary complex formation. Guanosine Triphosphate 125-128 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 60-63 33762326-4 2021 In this study, we show that the PP2A regulatory subunit PPP2R2A is increased in T cells from people with systemic lupus erythematosus and binds to, dephosphorylates, and activates the guanine nucleotide exchange factor GEF-H1 at Ser885, which in turn increases the levels of RhoA-GTP and the activity of ROCK in T cells. Guanosine Triphosphate 280-283 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 219-225 3462720-6 1986 (ii) In the presence of Mg2+, the GEF-catalyzed formation of a ternary complex (eIF-2 X GTP X Met-tRNAi in which Met-tRNAi is the eukaryotic initiator methionyl tRNA) is inhibited by eIF-2 X PL just as well as by eIF-2 alpha-subunit phosphorylation. Guanosine Triphosphate 88-91 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 34-37 8049218-11 1994 In the presence of 1 mM Mg2+, the amount of GTP bound to dephosphorylated GEF is reduced 3.8-fold as compared to that of the CK-II-phosphorylated GEF. Guanosine Triphosphate 44-47 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 74-77 3947361-1 1986 A guanine nucleotide exchange factor (GEF), catalyzing the exchange of GDP bound to initiation factor eIF-2 for GTP, has been isolated from S3 HeLa cells as the eIF-2 X GEF complex and extensively purified by procedures originally developed for purification of GEF from rabbit reticulocytes. Guanosine Triphosphate 112-115 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 169-172 3947361-1 1986 A guanine nucleotide exchange factor (GEF), catalyzing the exchange of GDP bound to initiation factor eIF-2 for GTP, has been isolated from S3 HeLa cells as the eIF-2 X GEF complex and extensively purified by procedures originally developed for purification of GEF from rabbit reticulocytes. Guanosine Triphosphate 112-115 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 169-172 27558716-1 2016 Resistance to Inhibitors of Cholinesterase A (Ric-8A) is a 60-kDa cytosolic protein that has chaperone and guanine nucleotide exchange (GEF) activity toward heterotrimeric G protein alpha subunits of the i, q, and 12/13 classes, catalyzing the release of GDP from Galpha and subsequent binding of GTP. Guanosine Triphosphate 297-300 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 136-139 29718126-5 2018 Characterization of hundreds of individual tumor cells identified three mutated genes within single cells, involved in the RAS/GEF GTP-dependent signaling pathway. Guanosine Triphosphate 131-134 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 127-130 30979377-2 2019 Such effects are partly due to the inactivation of the anti-migratory RhoB GTPase via the inhibitory phosphorylation of GEF-H1, the GDP/GTP exchange factor for RhoB. Guanosine Triphosphate 75-78 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 120-126 29106825-10 2017 In conclusion, we report two recurrent dominant mutations in RAB11B leading to a neurodevelopmental syndrome, likely caused by altered GDP/GTP binding that inactivate the protein and induce GEF binding and protein mislocalization. Guanosine Triphosphate 139-142 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 190-193 28706196-0 2017 Synaptic GAP and GEF Complexes Cluster Proteins Essential for GTP Signaling. Guanosine Triphosphate 62-65 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 17-20 26195453-6 2015 We used the Transcreener GDP Assay, which relies on selective immunodetection of GDP, to measure the GEF-dependent stimulation of steady-state GTP hydrolysis by small GTPases using Dbs (Dbl"s big sister) as a GEF for Cdc42, RhoA, and RhoB. Guanosine Triphosphate 143-146 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 101-104 27458535-2 2016 This GEF increases active Ras as it catalyzes the removal of GDP from Ras so that GTP can bind and activate Ras. Guanosine Triphosphate 82-85 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 5-8 26195453-6 2015 We used the Transcreener GDP Assay, which relies on selective immunodetection of GDP, to measure the GEF-dependent stimulation of steady-state GTP hydrolysis by small GTPases using Dbs (Dbl"s big sister) as a GEF for Cdc42, RhoA, and RhoB. Guanosine Triphosphate 143-146 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 209-212 25707436-9 2015 The method allows studying the GEF dependent H-Ras activation (GTP binding) and GAP-catalyzed H-Ras deactivation (GTP hydrolysis) at nanomolar protein concentrations. Guanosine Triphosphate 63-66 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 31-34 26488653-12 2015 Since a specific Arl3-GEF is postulated to reside inside cilia, the N-terminal helix of Arl3 GTP would be available for allosteric regulation of UNC119a cargo release only inside cilia. Guanosine Triphosphate 93-96 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 22-25 26304119-7 2015 In accordance with the necessity of a GEF to promote GTP-binding and nuclear export of Ran, the nuclear localization of Ran was strongly increased in MYCBP2-deficient DRGs. Guanosine Triphosphate 53-56 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 38-41 25825487-5 2015 From a library of 350,000 compounds, we selected a set of 418 candidate compounds predicted to disrupt the GEF-Ras interaction, of which dual wavelength GDP dissociation and GTP-loading experimental screening identified two chemically distinct small molecule inhibitors. Guanosine Triphosphate 174-177 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 107-110 24597981-1 2014 Rasgrf-1 is a guanine exchange factor (GEF) that catalyzes the exchange of GDP for GTP. Guanosine Triphosphate 83-86 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 14-37 24597981-1 2014 Rasgrf-1 is a guanine exchange factor (GEF) that catalyzes the exchange of GDP for GTP. Guanosine Triphosphate 83-86 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 39-42 23076143-5 2012 We recently reported that the Rho-GEF Trio, known to be able to exchange GTP on Rac1, RhoG and RhoA, regulates lamellipodia formation to mediate cell spreading and migration in a Rac1-dependent manner. Guanosine Triphosphate 73-76 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 34-37 24760397-7 2014 After GEF catalyzed GTP-loading of a GTPase, a high time-resolved luminescence signal was found to be associated with GTPase bound Eu-GTP, whereas the non-bound Eu-GTP fraction was quenched by soluble quencher. Guanosine Triphosphate 20-23 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 6-9 24760397-7 2014 After GEF catalyzed GTP-loading of a GTPase, a high time-resolved luminescence signal was found to be associated with GTPase bound Eu-GTP, whereas the non-bound Eu-GTP fraction was quenched by soluble quencher. Guanosine Triphosphate 37-40 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 6-9 22960035-1 2013 We report the novel finding that Phospholipase D2 (PLD2), through its PX and PH domains, binds specifically to Ras and catalyzes the GDP/GTP exchange (i.e., is a GEF), with potency comparable to Ras-GRF-1, a known Ras-GEF. Guanosine Triphosphate 137-140 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 162-165 22960035-1 2013 We report the novel finding that Phospholipase D2 (PLD2), through its PX and PH domains, binds specifically to Ras and catalyzes the GDP/GTP exchange (i.e., is a GEF), with potency comparable to Ras-GRF-1, a known Ras-GEF. Guanosine Triphosphate 137-140 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 218-221 22847784-11 2012 In addition, reduced active form of GTP-RhoA, together with its downstream effectors, including cleaved ROCK1 and phosphorylated MLC2, were also detected in GEF-H1-depleted cells. Guanosine Triphosphate 36-39 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 157-163 22825554-5 2012 Upon binding, GTP-loaded R-Ras converts RIN2 from a Rab5 guanine nucleotide exchange factor (GEF) to an adaptor that first interacts at high affinity with Rab5-GTP to promote the selective endocytosis of ligand-bound/active beta1 integrins and then causes the translocation of R-Ras to early endosomes. Guanosine Triphosphate 14-17 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 93-96 22291991-5 2012 Here we found that RINL has GEF activity for the Rab5 subfamily proteins by measuring their GTP-bound forms in cultured cells. Guanosine Triphosphate 92-95 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 28-31 19356586-7 2009 We suggest a novel model for selective nucleotide exchange residing on a conformational change of Cdc42 upon binding of GTP, which enables effector binding to the Cdc42.GTP complex but, at the same time, excludes efficient modulation by the GEF. Guanosine Triphosphate 120-123 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 241-244 22673745-4 2012 Direct participation of residue 43 in GEF-catalyzed exchange was confirmed by the observation that mutation of this position to a threonine reduced GEF-catalyzed nucleotide exchange activity in vitro (Vav2, XPLN, GEFT, Dbl and Dbs) and greatly depressed RhoA and RhoC GTP-loading profiles in cell lysates. Guanosine Triphosphate 268-271 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 38-41 22673745-4 2012 Direct participation of residue 43 in GEF-catalyzed exchange was confirmed by the observation that mutation of this position to a threonine reduced GEF-catalyzed nucleotide exchange activity in vitro (Vav2, XPLN, GEFT, Dbl and Dbs) and greatly depressed RhoA and RhoC GTP-loading profiles in cell lysates. Guanosine Triphosphate 268-271 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 148-151 21828055-8 2011 Our structural, cell biological, and biochemical findings identify loop>J as a key regulatory motif essential for ARF binding and GDP to GTP exchange by GEFs and provide evidence for the requirement of membrane association during GEF activity. Guanosine Triphosphate 140-143 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 156-159 21586568-7 2011 RIN3 stimulated the formation of GTP-bound Rab31 in cell-free and in cell GEF activity assays. Guanosine Triphosphate 33-36 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 74-77 21494904-1 2011 The guanine-nucleotide exchange factor (GEF) RalGPS1a activates small GTPase Ral proteins such as RalA and RalB by stimulating the exchange of Ral bound GDP to GTP, thus regulating various downstream cellular processes. Guanosine Triphosphate 70-73 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 40-43 19745154-5 2009 These studies identify an unusual mechanism of GDP release and define the complete GEF catalytic cycle from GDP dissociation followed by GTP binding and discharge of the activated GTPase. Guanosine Triphosphate 137-140 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 83-86 21454697-4 2011 This process is expected to require the participation of a guanine nucleotide exchange factor (GEF) to generate the GTP-bound form of Rab10, but this GEF has not hitherto been identified. Guanosine Triphosphate 116-119 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 95-98 21454697-4 2011 This process is expected to require the participation of a guanine nucleotide exchange factor (GEF) to generate the GTP-bound form of Rab10, but this GEF has not hitherto been identified. Guanosine Triphosphate 116-119 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 150-153 21390270-3 2011 For all the considered systems, the intrinsic flexibility of S(GDP) was higher than that of S(GTP), suggesting that Guanine Exchange Factor (GEF) recognition and nucleotide switch require higher amplitude motions than effector recognition or GTP hydrolysis. Guanosine Triphosphate 94-97 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 116-139 21390270-3 2011 For all the considered systems, the intrinsic flexibility of S(GDP) was higher than that of S(GTP), suggesting that Guanine Exchange Factor (GEF) recognition and nucleotide switch require higher amplitude motions than effector recognition or GTP hydrolysis. Guanosine Triphosphate 94-97 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 141-144 21390270-3 2011 For all the considered systems, the intrinsic flexibility of S(GDP) was higher than that of S(GTP), suggesting that Guanine Exchange Factor (GEF) recognition and nucleotide switch require higher amplitude motions than effector recognition or GTP hydrolysis. Guanosine Triphosphate 242-245 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 141-144 19361519-2 2009 Here we measured rate and equilibrium constants that define the interaction of Ypt1p with guanine nucleotide (guanosine 5"-diphosphate and guanosine 5"-triphosphate/guanosine 5"-(beta,gamma-imido)triphosphate) and the core TRAPP subunits required for GEF activity. Guanosine Triphosphate 139-164 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 251-254 19356586-7 2009 We suggest a novel model for selective nucleotide exchange residing on a conformational change of Cdc42 upon binding of GTP, which enables effector binding to the Cdc42.GTP complex but, at the same time, excludes efficient modulation by the GEF. Guanosine Triphosphate 169-172 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 241-244 19356586-8 2009 The higher exchange activity of ITSN1L towards the GDP-bound conformation of Cdc42 could represent an evolutionary adaptation of this GEF that ensures nucleotide exchange towards the formation of the signalling-active GTP-bound form of Cdc42 and avoids dissociation of the active complex. Guanosine Triphosphate 218-221 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 134-137 18505730-10 2008 Dissociation of Rac1(GDP).RhoGDI complexes was correlated with the affinity of particular GEF constructs, via the N-terminal pleckstrin homology domain, for PtdIns(3,4,5)P(3) and involved GEF-mediated GDP to GTP exchange on Rac1. Guanosine Triphosphate 208-211 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 90-93 15850775-2 2005 Here we report that TrioN, a Dbl family GEF, activates Rac1 by facilitating GTP binding to, as well as stimulating GDP dissociation from, Rac1. Guanosine Triphosphate 76-79 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 40-43 18347012-1 2008 Microtubule spindle assembly in mitosis is stimulated by Ran.GTP, which is generated along condensed chromosomes by the guanine nucleotide exchange factor (GEF) RCC1. Guanosine Triphosphate 61-64 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 156-159 17593019-2 2007 Ric8a was shown to be a GEF (guanine nucleotide exchange factor) for several alpha subunits of heterotrimeric GTP binding proteins (Galpha proteins) in vitro. Guanosine Triphosphate 110-113 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 24-27 16257999-3 2006 GEF-H1 is an MT-associated Rho-specific guanosine nucleotide (GDP/GTP) exchange factor, which in MT-unbound state stimulates Rho activity. Guanosine Triphosphate 66-69 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 0-6 17488622-5 2007 Dephosphorylation of GEF-H1 occurs just prior to cytokinesis, accompanied by GEF-H1-dependent GTP loading on RhoA. Guanosine Triphosphate 94-97 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 21-27 17488622-5 2007 Dephosphorylation of GEF-H1 occurs just prior to cytokinesis, accompanied by GEF-H1-dependent GTP loading on RhoA. Guanosine Triphosphate 94-97 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 77-83 16472645-7 2006 On the other hand, our structural and biochemical analysis of Rac1b has shown that, compared with Rac1, Rac1b has an accelerated GEF-independent GDP/GTP-exchange and an impaired GTP-hydrolysis, accounting for a self-activating GTPase. Guanosine Triphosphate 149-152 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 129-132 16248653-4 2005 In some GNBP families, the release of Mg(2+) was reported to play an important role in binding the guanine nucleotide-exchanging factor (GEF) promoting the GDP/GTP exchange reaction. Guanosine Triphosphate 160-163 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 99-135 16248653-4 2005 In some GNBP families, the release of Mg(2+) was reported to play an important role in binding the guanine nucleotide-exchanging factor (GEF) promoting the GDP/GTP exchange reaction. Guanosine Triphosphate 160-163 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 137-140 15850775-4 2005 The TrioN-stimulated nucleotide exchange may undergo a novel two nucleotide-one G-protein intermediate involving two cryptic subsites on Rac1 induced by the GEF, with one subsite contributing to the recognition of the beta/gamma phosphates of the incoming GTP and another to the binding of the guanine base of the leaving GDP. Guanosine Triphosphate 256-259 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 157-160 15850775-5 2005 We propose that the Rac GEF reaction may proceed by competitive displacement of bound GDP by GTP through a transient intermediate of GEF-[GTP-Rac-GDP]. Guanosine Triphosphate 93-96 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 24-27 15850775-5 2005 We propose that the Rac GEF reaction may proceed by competitive displacement of bound GDP by GTP through a transient intermediate of GEF-[GTP-Rac-GDP]. Guanosine Triphosphate 93-96 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 133-136 15850775-5 2005 We propose that the Rac GEF reaction may proceed by competitive displacement of bound GDP by GTP through a transient intermediate of GEF-[GTP-Rac-GDP]. Guanosine Triphosphate 138-141 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 24-27 15850775-5 2005 We propose that the Rac GEF reaction may proceed by competitive displacement of bound GDP by GTP through a transient intermediate of GEF-[GTP-Rac-GDP]. Guanosine Triphosphate 138-141 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 133-136 15709769-4 2005 Herein we have examined in detail the catalyzed GDP/GTP exchange reaction mechanism by a Ras specific GEF, GRF1. Guanosine Triphosphate 52-55 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 102-105 15709769-9 2005 These results strongly support a GEF reaction mechanism by which nucleotide exchange occurs on Ras through a direct GTP/GDP displacement model. Guanosine Triphosphate 116-119 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 33-36 15709769-6 2005 The release of the Ras-bound GDP was dependent upon the concentration and the structure of the incoming nucleotide, in particular, the hydrophobicity of the beta and gamma phosphate groups, suggesting that the GTP binding step is a prerequisite for GDP dissociation, is the rate-limiting step in the GEF reaction, or both. Guanosine Triphosphate 210-213 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 300-303 14625275-3 2004 As a consequence, Rac1b has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. Guanosine Triphosphate 63-66 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 43-46 15544329-2 2004 The current biochemical model postulates that the GEF-stimulated GDP/GTP exchange of Rho GTPases leads to the active Rho-GTP species, and subsequently the active Rho GTPases interact with and activate the effectors. Guanosine Triphosphate 69-72 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 50-53 15544329-2 2004 The current biochemical model postulates that the GEF-stimulated GDP/GTP exchange of Rho GTPases leads to the active Rho-GTP species, and subsequently the active Rho GTPases interact with and activate the effectors. Guanosine Triphosphate 89-92 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 50-53 15544329-8 2004 Finally, PAK1, through the PDB domain, is able to accelerate the GEF-induced GTP loading onto Cdc42. Guanosine Triphosphate 77-80 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 65-68 12680752-5 2003 The GEF sensors yield up to 1.7-fold changes in FRET upon exchange of GDP for GTP. Guanosine Triphosphate 78-81 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 4-7 14597635-4 2004 Based on the available structural information, we have identified the highly conserved amino acid pairing of Asn(1406)Trio-Asp(65)Rac1 of the GEF-Rho GTPase interaction as the critical catalytic machinery required for the Rac1 GDP/GTP exchange reaction. Guanosine Triphosphate 150-153 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 142-145 14712228-6 2004 Deletion of the C-terminal tail of PDZ-RhoGEF had no significant effect on the GEF catalytic activity towards Rho in vitro, but resulted in a drastic increase in the ability to stimulate a serum response element reporter and the accumulation of the GTP-bound Rho in vivo. Guanosine Triphosphate 249-252 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 42-45 12641750-1 2003 msec7-1, a mammalian homologue of yeast sec7p, is known as a GDP/GTP exchange factor (GEF) for the ADP ribosylation factor (ARF) family of small GTPases. Guanosine Triphosphate 65-68 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 86-89 11087745-0 2001 Phospholipase Cdelta1 is a guanine nucleotide exchanging factor for transglutaminase II (Galpha h) and promotes alpha 1B-adrenoreceptor-mediated GTP binding and intracellular calcium release. Guanosine Triphosphate 145-148 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 27-63 11087745-6 2001 These results indicate that PLCdelta1 is GEF for TGII and stabilizes the GTP.TGII complex. Guanosine Triphosphate 73-76 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 41-44 9790530-2 1998 The crystal structure of the Sec7 domain GEF bound to nucleotide-free ARF1 GTPase has been determined at 2.8 A resolution and the structure of ARF1 in the GTP-analog form determined at 1.6 A resolution. Guanosine Triphosphate 75-78 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 41-44 9763446-6 1998 The Ypt1p-GEF stimulates GDP release and GTP uptake at least 10-fold and is specific for Ypt1p. Guanosine Triphosphate 41-44 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 10-13