PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 8702675-1 1996 Ral GDP dissociation stimulator (RalGDS) is a GDP/GTP exchange protein of Ral and a new effector protein of Ras. Guanosine Triphosphate 50-53 RAS like proto-oncogene A Homo sapiens 0-3 9188503-0 1997 Identification and characterization of a calmodulin-binding domain in Ral-A, a Ras-related GTP-binding protein purified from human erythrocyte membrane. Guanosine Triphosphate 91-94 RAS like proto-oncogene A Homo sapiens 70-75 9188503-2 1997 Based on internal peptide sequencing and its protein amino acid composition, this protein has been shown to be highly related, if not identical to, Ral-A, a Ras-related GTP-binding protein. Guanosine Triphosphate 169-172 RAS like proto-oncogene A Homo sapiens 148-153 8702675-6 1996 The post-translational modification of Ral enhanced the activities of RalGDS to stimulate the dissociation of GDP from and the binding of GTP to Ral. Guanosine Triphosphate 138-141 RAS like proto-oncogene A Homo sapiens 39-42 8702675-6 1996 The post-translational modification of Ral enhanced the activities of RalGDS to stimulate the dissociation of GDP from and the binding of GTP to Ral. Guanosine Triphosphate 138-141 RAS like proto-oncogene A Homo sapiens 70-73 8664345-5 1996 Analysis of GTPase reaction products by thin layer chromatography demonstrated that in samples containing ralA, 78.5 +/- 6.3% of the radioactivity was recovered in the GTP form while samples containing ralA plus platelet cytosol or particulate proteins, only 7.5 +/- 0.2% and 9.0 +/- 1.4% of the radioactivity was in the GTP form respectively. Guanosine Triphosphate 12-15 RAS like proto-oncogene A Homo sapiens 106-110 8636102-0 1996 Differential interaction of the ras family GTP-binding proteins H-Ras, Rap1A, and R-Ras with the putative effector molecules Raf kinase and Ral-guanine nucleotide exchange factor. Guanosine Triphosphate 43-46 RAS like proto-oncogene A Homo sapiens 140-143 8756332-1 1996 Members of the Ras subfamily of small GTP-binding proteins have been shown to be promiscuous towards a variety of putative effector molecules such as the protein kinase c-Raf and the Ral-specific guanine nucleotide exchange factor (Ral-GEF). Guanosine Triphosphate 38-41 RAS like proto-oncogene A Homo sapiens 183-186 8756332-1 1996 Members of the Ras subfamily of small GTP-binding proteins have been shown to be promiscuous towards a variety of putative effector molecules such as the protein kinase c-Raf and the Ral-specific guanine nucleotide exchange factor (Ral-GEF). Guanosine Triphosphate 38-41 RAS like proto-oncogene A Homo sapiens 232-235 8664345-5 1996 Analysis of GTPase reaction products by thin layer chromatography demonstrated that in samples containing ralA, 78.5 +/- 6.3% of the radioactivity was recovered in the GTP form while samples containing ralA plus platelet cytosol or particulate proteins, only 7.5 +/- 0.2% and 9.0 +/- 1.4% of the radioactivity was in the GTP form respectively. Guanosine Triphosphate 168-171 RAS like proto-oncogene A Homo sapiens 106-110 8570186-2 1995 RIP1 binds to Ral in a GTP-dependent manner. Guanosine Triphosphate 23-26 RAS like proto-oncogene A Homo sapiens 14-17 7623849-5 1995 RalBP1 binds specifically to the active GTP-bound form of RalA and not to a mutant Ral with a point mutation in its putative effector domain. Guanosine Triphosphate 40-43 RAS like proto-oncogene A Homo sapiens 0-3 34767674-1 2021 BACKGROUND AND AIMS: Ras-like (Ral) small GTPases, RalA and RalB, are proto-oncogenes directly downstream of Ras and cycle between the active GTP-bound and inactive GDP-bound forms. Guanosine Triphosphate 142-145 RAS like proto-oncogene A Homo sapiens 51-55 7925476-2 1994 Recombinant c-Ha-ras, ralA and rap2, but not rap1A or rap1B proteins retained their ability to bind [alpha-32P]GTP after SDS/PAGE and transfer to nitrocellulose. Guanosine Triphosphate 111-114 RAS like proto-oncogene A Homo sapiens 22-26 1903399-0 1991 Carboxyl-terminal isoprenylation of ras-related GTP-binding proteins encoded by rac1, rac2, and ralA. Guanosine Triphosphate 48-51 RAS like proto-oncogene A Homo sapiens 96-100 1903399-3 1991 We now show that three GTP-binding proteins encoded by the recently identified rac1, rac2, and ralA genes also undergo isoprenoid modification. Guanosine Triphosphate 23-26 RAS like proto-oncogene A Homo sapiens 95-99 8373799-0 1993 Ral and Rab3a are major GTP-binding proteins of axonal rapid transport and synaptic vesicles and do not redistribute following depolarization stimulated synaptosomal exocytosis. Guanosine Triphosphate 24-27 RAS like proto-oncogene A Homo sapiens 0-3 30500825-0 2018 De novo mutations in the GTP/GDP-binding region of RALA, a RAS-like small GTPase, cause intellectual disability and developmental delay. Guanosine Triphosphate 25-28 RAS like proto-oncogene A Homo sapiens 51-55 34074494-2 2021 RAL GTPases function as molecular switches in cells by cycling through GDP- and GTP-bound states, a process which is regulated by several guanine exchange factors (GEFs) and two heterodimeric GTPase activating proteins (GAPs). Guanosine Triphosphate 80-83 RAS like proto-oncogene A Homo sapiens 0-3 2550440-0 1989 Identification of the ral and rac1 gene products, low molecular mass GTP-binding proteins from human platelets. Guanosine Triphosphate 69-72 RAS like proto-oncogene A Homo sapiens 22-25 2550440-11 1989 These results identify two new GTP-binding proteins in human platelets, ral, the major protein that binds [alpha-32P]GTP on nitrocellulose transfers, and rac1, a substrate for botulinum C3 ADP-ribosyltransferase. Guanosine Triphosphate 31-34 RAS like proto-oncogene A Homo sapiens 72-75 31916136-2 2020 RalA and RalB are activated downstream of the master regulator, Ras, which causes the nucleotide exchange of GDP for GTP. Guanosine Triphosphate 117-120 RAS like proto-oncogene A Homo sapiens 0-4 31916136-3 2020 Here we report the 1H, 15 N and 13C resonance assignments of RalA in its active form bound to the GTP analogue GMPPNP. Guanosine Triphosphate 98-101 RAS like proto-oncogene A Homo sapiens 61-65 3023062-6 1986 The GTP binding regions of p21 ras and a C-terminal cysteine involved in membrane anchoring are also present in ral; this strongly suggests that ral is a GTP binding protein with membrane localization. Guanosine Triphosphate 4-7 RAS like proto-oncogene A Homo sapiens 112-115 3023062-6 1986 The GTP binding regions of p21 ras and a C-terminal cysteine involved in membrane anchoring are also present in ral; this strongly suggests that ral is a GTP binding protein with membrane localization. Guanosine Triphosphate 4-7 RAS like proto-oncogene A Homo sapiens 145-148 3023062-6 1986 The GTP binding regions of p21 ras and a C-terminal cysteine involved in membrane anchoring are also present in ral; this strongly suggests that ral is a GTP binding protein with membrane localization. Guanosine Triphosphate 154-157 RAS like proto-oncogene A Homo sapiens 112-115 3023062-6 1986 The GTP binding regions of p21 ras and a C-terminal cysteine involved in membrane anchoring are also present in ral; this strongly suggests that ral is a GTP binding protein with membrane localization. Guanosine Triphosphate 154-157 RAS like proto-oncogene A Homo sapiens 145-148 30500825-6 2018 Further, all de novo variants described here affect residues within the GTP/GDP-binding region of RALA; in fact, six alleles arose at only two codons, Val25 and Lys128. Guanosine Triphosphate 72-75 RAS like proto-oncogene A Homo sapiens 98-102 27334922-6 2016 We have used our structure of a Ral-effector complex as a basis for the design and characterization of alpha-helical-stapled peptides that bind selectively to active, GTP-bound Ral proteins and that compete with downstream effector proteins. Guanosine Triphosphate 167-170 RAS like proto-oncogene A Homo sapiens 32-35 28744424-7 2017 From these findings, we infer that Ras activation not merely increases the cell-surface density of RalGDS, but actively stimulates the RalGDS-Ral interaction through a structural change in RalGDS and/or the accumulation of Ral, as well as the GTP-Ras/RalGDS clusters, to induce the full activation of Ral. Guanosine Triphosphate 243-246 RAS like proto-oncogene A Homo sapiens 135-138 28744424-7 2017 From these findings, we infer that Ras activation not merely increases the cell-surface density of RalGDS, but actively stimulates the RalGDS-Ral interaction through a structural change in RalGDS and/or the accumulation of Ral, as well as the GTP-Ras/RalGDS clusters, to induce the full activation of Ral. Guanosine Triphosphate 243-246 RAS like proto-oncogene A Homo sapiens 135-138 27773821-1 2016 RalGDS is a guanine nucleotide exchange factor that promotes the active GTP-bound form of Ral GTPases, RalA and RalB. Guanosine Triphosphate 72-75 RAS like proto-oncogene A Homo sapiens 0-3 27773821-2 2016 GTP-bound Ras has the capacity to activate Ral GTPases at least in part by binding to the C-terminal Ras-binding domain (RBD) of RalGDS and directing the protein to Ral GTPases in the plasma membrane. Guanosine Triphosphate 0-3 RAS like proto-oncogene A Homo sapiens 43-46 27773821-2 2016 GTP-bound Ras has the capacity to activate Ral GTPases at least in part by binding to the C-terminal Ras-binding domain (RBD) of RalGDS and directing the protein to Ral GTPases in the plasma membrane. Guanosine Triphosphate 0-3 RAS like proto-oncogene A Homo sapiens 129-132 27334922-6 2016 We have used our structure of a Ral-effector complex as a basis for the design and characterization of alpha-helical-stapled peptides that bind selectively to active, GTP-bound Ral proteins and that compete with downstream effector proteins. Guanosine Triphosphate 167-170 RAS like proto-oncogene A Homo sapiens 177-180 26477566-3 2016 Among various small G proteins tested, GTP-bound form (G23V) of RalA inhibited the internalization of dopamine D2 receptor independently of the previously reported downstream effectors of RalA, such as Ral-binding protein 1 and PLD. Guanosine Triphosphate 39-42 RAS like proto-oncogene A Homo sapiens 64-68 26477566-5 2016 When it was tested for several GPCRs including an endogenous GPCR, lysophosphatidic acid receptor 1, agonist-induced conversion of GTP-bound to GDP-bound RalA, which presumably releases the sequestered GRK2, was observed selectively with the GPCRs which have tendency to undergo endocytosis. Guanosine Triphosphate 131-134 RAS like proto-oncogene A Homo sapiens 154-158 26477566-7 2016 These results suggest that agonist-induced Gbetagamma-mediated conversion of RalA from the GTP-bound form to the GDP-bound form could be a mechanism to facilitate agonist-induced internalization of GPCRs. Guanosine Triphosphate 91-94 RAS like proto-oncogene A Homo sapiens 77-81 26425463-2 2015 These isoprenoid intermediates are required for activation of various intracellular/signaling proteins- small guanosine triphosphate bound protein Ras and Ras-like proteins like Rho, Rab, Rac, Ral, or Rap which plays an indispensible role in multiple cellular processes. Guanosine Triphosphate 110-132 RAS like proto-oncogene A Homo sapiens 193-196 26158537-6 2015 Importantly, several mitotic phenotypes caused by TD-60 depletion are reverted by the expression of a GTP-locked mutant, RalA (Q72L). Guanosine Triphosphate 102-105 RAS like proto-oncogene A Homo sapiens 121-125 26033452-3 2015 Knocking-down RALA expression strongly diminished the active GTP-bound form of the protein. Guanosine Triphosphate 61-64 RAS like proto-oncogene A Homo sapiens 14-18 25213293-1 2015 RLIP76, a multidomain protein which is a downstream effector of the small GTP ases RalA and RalB, is known to play a role in biological activities in a variety of malignant cancer cells. Guanosine Triphosphate 74-77 RAS like proto-oncogene A Homo sapiens 83-87 25103239-4 2014 Once activated, Rab10 can increase the GTP binding of RalA by recruiting the Ral guanyl nucleotide exchange factor, Rlf/Rgl2. Guanosine Triphosphate 39-42 RAS like proto-oncogene A Homo sapiens 54-58 25169936-3 2014 APPROACH AND RESULTS: In addition to conventional markers, GTP-bound levels of Rap1, RhoA, RalA, Rac1, and Ras in platelets, which are implicated for platelet activation, were measured in patients without pulmonary hypertension (non-PH, n=15), patients with PAH (n=19), and patients with CTEPH (n=25). Guanosine Triphosphate 59-62 RAS like proto-oncogene A Homo sapiens 91-95 25431955-1 2014 The Ral (Ras-like) GTP-binding proteins (RalA and RalB), as effectors of the proto-oncogene Natural killer (NK) cells are an important component of the anti-tumor response. Guanosine Triphosphate 19-22 RAS like proto-oncogene A Homo sapiens 4-7 21242975-8 2011 We have also observed that CycD1-Cdk4 phosphorylates the Ral GEF Rgl2 "in vitro" and that CycD1-Cdk4 activity stimulates accumulation of the Ral GTP active forms. Guanosine Triphosphate 145-148 RAS like proto-oncogene A Homo sapiens 57-60 22700969-7 2012 This study suggests the existence of an ubiquitination/de-ubiquitination cycle superimposed on the GDP/GTP cycle of RalA, involved in the regulation of RalA activity as well as in membrane raft trafficking. Guanosine Triphosphate 103-106 RAS like proto-oncogene A Homo sapiens 116-120 22700969-7 2012 This study suggests the existence of an ubiquitination/de-ubiquitination cycle superimposed on the GDP/GTP cycle of RalA, involved in the regulation of RalA activity as well as in membrane raft trafficking. Guanosine Triphosphate 103-106 RAS like proto-oncogene A Homo sapiens 152-156 23226417-6 2012 Mutations in the switch II region of the GTPase domain of RalA specifically reduced or abolished binding to ERp57, but did not block GTP-specific binding to known RalA effectors, the exocyst and RalBP1. Guanosine Triphosphate 41-44 RAS like proto-oncogene A Homo sapiens 58-62 21242975-8 2011 We have also observed that CycD1-Cdk4 phosphorylates the Ral GEF Rgl2 "in vitro" and that CycD1-Cdk4 activity stimulates accumulation of the Ral GTP active forms. Guanosine Triphosphate 145-148 RAS like proto-oncogene A Homo sapiens 141-144 18948269-4 2008 The amount of GTP-bound RalA increased in response to increased amino acid availability. Guanosine Triphosphate 14-17 RAS like proto-oncogene A Homo sapiens 24-28 21235523-6 2011 We will also review the function of the exocyst complex and some of its key interacting partners [including the small GTP-binding protein Ral, aPKCs (atypical protein kinase Cs) and proteins involved in actin assembly] in the formation of plasma extensions at the leading edge, growth cone formation during axonal extension and generation of cell movement. Guanosine Triphosphate 118-121 RAS like proto-oncogene A Homo sapiens 138-141 21148297-4 2011 The complex is composed of an RGC1 regulatory subunit and an RGC2 catalytic subunit (previously identified as AS250) that directly stimulates the guanosine triphosphate hydrolysis of RalA. Guanosine Triphosphate 146-168 RAS like proto-oncogene A Homo sapiens 183-187 20562921-5 2010 The only major Ras effector pathway not explored for its role in melanoma is the RalGEF-Ral pathway, in which Ras activation of RalGEFs converts the small GTPases RalA and RalB to an active guanosine triphosphate-bound state. Guanosine Triphosphate 190-212 RAS like proto-oncogene A Homo sapiens 81-84 20562921-5 2010 The only major Ras effector pathway not explored for its role in melanoma is the RalGEF-Ral pathway, in which Ras activation of RalGEFs converts the small GTPases RalA and RalB to an active guanosine triphosphate-bound state. Guanosine Triphosphate 190-212 RAS like proto-oncogene A Homo sapiens 163-167 21494904-1 2011 The guanine-nucleotide exchange factor (GEF) RalGPS1a activates small GTPase Ral proteins such as RalA and RalB by stimulating the exchange of Ral bound GDP to GTP, thus regulating various downstream cellular processes. Guanosine Triphosphate 70-73 RAS like proto-oncogene A Homo sapiens 45-48 21494904-1 2011 The guanine-nucleotide exchange factor (GEF) RalGPS1a activates small GTPase Ral proteins such as RalA and RalB by stimulating the exchange of Ral bound GDP to GTP, thus regulating various downstream cellular processes. Guanosine Triphosphate 70-73 RAS like proto-oncogene A Homo sapiens 98-102 21494904-1 2011 The guanine-nucleotide exchange factor (GEF) RalGPS1a activates small GTPase Ral proteins such as RalA and RalB by stimulating the exchange of Ral bound GDP to GTP, thus regulating various downstream cellular processes. Guanosine Triphosphate 70-73 RAS like proto-oncogene A Homo sapiens 77-80 20603646-3 2010 The active GTP-bound form of Ras has been known to associate with diverse effectors including Raf, phosphatidylinositol 3-kinase (PI3K), Ral-GDS, and other molecules to transmit downstream signals. Guanosine Triphosphate 11-14 RAS like proto-oncogene A Homo sapiens 137-140 20145037-3 2010 Activated RalGEFs convert the Ral family of small GTPases, composed of RalA and RalB, from an inactive GDP-bound state to an active GTP-bound state. Guanosine Triphosphate 50-53 RAS like proto-oncogene A Homo sapiens 10-13 20145037-3 2010 Activated RalGEFs convert the Ral family of small GTPases, composed of RalA and RalB, from an inactive GDP-bound state to an active GTP-bound state. Guanosine Triphosphate 50-53 RAS like proto-oncogene A Homo sapiens 71-75 16757319-4 2006 RalGEFs activate Ral by stimulating the dissociation of GDP, allowing the binding of GTP and the initiation of downstream signaling events by Ral effectors. Guanosine Triphosphate 85-88 RAS like proto-oncogene A Homo sapiens 0-3 17938170-3 2008 This secretion was inhibited by the addition of GTP-Ral-binding domain (RBD) of Sec5, which is a component of the exocyst complex known to function as a tethering factor at the plasma membrane for vesicles. Guanosine Triphosphate 48-51 RAS like proto-oncogene A Homo sapiens 52-55 17938170-4 2008 We generated an antibody against Sec5-RBD, which abolished the interaction between GTP-Ral and the exocyst complex in vitro. Guanosine Triphosphate 83-86 RAS like proto-oncogene A Homo sapiens 87-90 18417737-1 2008 The small GTP-binding protein Ral has been implicated in regulated exocytosis via its interaction with the mammalian exocyst complex. Guanosine Triphosphate 10-13 RAS like proto-oncogene A Homo sapiens 30-33 16757319-4 2006 RalGEFs activate Ral by stimulating the dissociation of GDP, allowing the binding of GTP and the initiation of downstream signaling events by Ral effectors. Guanosine Triphosphate 85-88 RAS like proto-oncogene A Homo sapiens 17-20 16757319-2 2006 One such member, Ral GTPase, activates downstream signaling molecules after a conversion to the active state on GTP binding. Guanosine Triphosphate 21-24 RAS like proto-oncogene A Homo sapiens 17-20 15470141-4 2004 We show here that the small GTP-binding protein Ral, its guanine nucleotide exchange factor RalGDS (Ral GDP dissociation stimulator), and phospholipase D2 (PLD2) are constitutively associated with class 1 mGluRs and regulate constitutive mGluR endocytosis. Guanosine Triphosphate 28-31 RAS like proto-oncogene A Homo sapiens 48-51 15592429-2 2005 To identify novel RalA effector proteins, we used the reverse Ras recruitment system and found that RalA interacts with a Y-box transcription factor, ZO-1-associated nucleic acid-binding protein (ZONAB), in a GTP-dependent manner. Guanosine Triphosphate 209-212 RAS like proto-oncogene A Homo sapiens 18-22 15592429-2 2005 To identify novel RalA effector proteins, we used the reverse Ras recruitment system and found that RalA interacts with a Y-box transcription factor, ZO-1-associated nucleic acid-binding protein (ZONAB), in a GTP-dependent manner. Guanosine Triphosphate 209-212 RAS like proto-oncogene A Homo sapiens 100-104 15130921-7 2004 Furthermore, inhibition of protein kinase A by H-89 resulted in a marked reduction of vWF release and greatly diminished levels of GTP-Ral on stimulation with epinephrine. Guanosine Triphosphate 131-134 RAS like proto-oncogene A Homo sapiens 135-138 12526085-8 2002 Ca(2+)/calmodulin (CaM), which causes the small GTP-binding proteins like Rab3A and RalA to dissociate from the membranes and stimulates CaM-dependent protein kinase(s) and phosphatase, strongly stimulate the phosphorylation of p46 in the presence of cyclosporin A and cyclophylin. Guanosine Triphosphate 48-51 RAS like proto-oncogene A Homo sapiens 84-88 15130921-0 2004 Small GTP-binding protein Ral is involved in cAMP-mediated release of von Willebrand factor from endothelial cells. Guanosine Triphosphate 6-9 RAS like proto-oncogene A Homo sapiens 26-29 15130921-2 2004 Recently, we have shown that the small GTP-binding protein Ral is involved in thrombin-induced exocytosis of WPBs. Guanosine Triphosphate 39-42 RAS like proto-oncogene A Homo sapiens 59-62 12839989-2 2003 The Sec5 subunit of the mammalian sec6/8 complex binds Ral in a GTP-dependent manner. Guanosine Triphosphate 64-67 RAS like proto-oncogene A Homo sapiens 55-58 12839989-3 2003 Here we report the crystal structure of the complex between the Ral-binding domain of Sec5 and RalA bound to a non-hydrolyzable GTP analog (GppNHp) at 2.1 A resolution, providing the first structural insights into the mechanism and specificity of sec6/8 regulation. Guanosine Triphosphate 128-131 RAS like proto-oncogene A Homo sapiens 95-99 11322487-0 2001 Regulation of GTP-binding state in RalA through Ca2+ and calmodulin. Guanosine Triphosphate 14-17 RAS like proto-oncogene A Homo sapiens 35-39 11406615-0 2001 The brain exocyst complex interacts with RalA in a GTP-dependent manner: identification of a novel mammalian Sec3 gene and a second Sec15 gene. Guanosine Triphosphate 51-54 RAS like proto-oncogene A Homo sapiens 41-45 11406615-4 2001 We found at least eight proteins that bound RalA in a GTP-dependent manner. Guanosine Triphosphate 54-57 RAS like proto-oncogene A Homo sapiens 44-48 11397694-0 2001 Small GTP-binding protein Ral modulates regulated exocytosis of von Willebrand factor by endothelial cells. Guanosine Triphosphate 6-9 RAS like proto-oncogene A Homo sapiens 26-29 11397694-2 2001 Recently, we found that the small GTP-binding protein Ral is present in a subcellular fraction containing Weibel-Palade bodies. Guanosine Triphosphate 34-37 RAS like proto-oncogene A Homo sapiens 54-57 11397694-4 2001 Activation of endothelial cells by thrombin resulted in transient cycling of Ral from its inactive GDP-bound to its active GTP-bound state, which coincided with release of vWF. Guanosine Triphosphate 123-126 RAS like proto-oncogene A Homo sapiens 77-80 12067705-6 2002 In contrast, monomeric GTP-binding protein Ral was translocated to membranes by S1P in a Ca(2+)-independent manner, ruling out its possible role in agonist-mediated regulation of PLD. Guanosine Triphosphate 23-26 RAS like proto-oncogene A Homo sapiens 43-46 11861368-3 2002 Here we show that EGF stimulates motility in human tumor cell lines, which harbor activating Ha-RasV12 via a novel signal transduction pathway mediated by the small GTP-binding proteins RalA and RhoA but independent of Rac1 and Cdc42. Guanosine Triphosphate 165-168 RAS like proto-oncogene A Homo sapiens 186-190 11744922-2 2002 We identified the mammalian homologue of Sec5, a subunit of the exocyst complex determining yeast cell polarity, as a specific binding partner for GTP-ligated RalA. Guanosine Triphosphate 147-150 RAS like proto-oncogene A Homo sapiens 159-163 11322487-1 2001 RalA GTPase, a member of Ras superfamily proteins, shows alternative forms between the active GTP-binding and the inactive GDP-binding states. Guanosine Triphosphate 5-8 RAS like proto-oncogene A Homo sapiens 0-4 11322487-4 2001 In this study, studies were carried out to examine possible effects of Ca2+ and calmodulin, Ca2+-binding protein, directly on the GTP/GDP-binding state to recombinant unprenylated GST-RalA proteins. Guanosine Triphosphate 130-133 RAS like proto-oncogene A Homo sapiens 184-188 11322487-5 2001 The results showed that Ca2+ stimulated the binding of GTP to RalA, whereas it reduced the binding of GDP to RalA. Guanosine Triphosphate 55-58 RAS like proto-oncogene A Homo sapiens 62-66 11322487-8 2001 These results indicate that Ca2+ alone activates RalA by stimulating GTP-binding to RalA and Ca2+/calmodulin inactivates RalA by increasing the activity of RalGTPase. Guanosine Triphosphate 69-72 RAS like proto-oncogene A Homo sapiens 49-53 11322487-8 2001 These results indicate that Ca2+ alone activates RalA by stimulating GTP-binding to RalA and Ca2+/calmodulin inactivates RalA by increasing the activity of RalGTPase. Guanosine Triphosphate 69-72 RAS like proto-oncogene A Homo sapiens 84-88 11322487-8 2001 These results indicate that Ca2+ alone activates RalA by stimulating GTP-binding to RalA and Ca2+/calmodulin inactivates RalA by increasing the activity of RalGTPase. Guanosine Triphosphate 69-72 RAS like proto-oncogene A Homo sapiens 84-88 10861233-2 2000 Here we report that LDL activates the small GTPases Rap1 and Ral but not Ras, as assessed by specific precipitation of the GTP-bound enzymes. Guanosine Triphosphate 44-47 RAS like proto-oncogene A Homo sapiens 61-64 10747847-4 2000 In vitro binding and in vivo nucleotide exchange assays indicate that these GEFs specifically catalyze the GTP loading of the Ral GTPase when overexpressed in 293T cells. Guanosine Triphosphate 107-110 RAS like proto-oncogene A Homo sapiens 126-129 10851075-5 2000 Rgr induces phosphorylation of ERKs, p38 and JNK kinases, and increases the levels of the GTP-bound forms of Ral and Ras. Guanosine Triphosphate 90-93 RAS like proto-oncogene A Homo sapiens 109-112 10364219-0 1999 Effector recognition by the small GTP-binding proteins Ras and Ral. Guanosine Triphosphate 34-37 RAS like proto-oncogene A Homo sapiens 63-66 10494784-0 1999 Small GTP-binding protein RalA associates with Weibel-Palade bodies in endothelial cells. Guanosine Triphosphate 6-9 RAS like proto-oncogene A Homo sapiens 26-30 10494784-10 1999 A monoclonal antibody directed against RalA showed reactivity with the small GTP-binding protein present in subcellular fractions that contain Weibel-Palade bodies. Guanosine Triphosphate 77-80 RAS like proto-oncogene A Homo sapiens 39-43 11455956-2 2000 RalGDS can specifically promote the conversion from an inactive GDP-bound state to an active GTP-bound state for Ral. Guanosine Triphosphate 93-96 RAS like proto-oncogene A Homo sapiens 0-3 10512754-2 1999 RalA belongs to the superfamily of Ras-related small GTP-binding proteins. Guanosine Triphosphate 53-56 RAS like proto-oncogene A Homo sapiens 0-4 10512754-3 1999 Synaptic vesicles (SV) contain small GTP-binding proteins, where RalA, Rab3A, and Rab5A are the major GTP-binding proteins. Guanosine Triphosphate 37-40 RAS like proto-oncogene A Homo sapiens 65-69 10512754-3 1999 Synaptic vesicles (SV) contain small GTP-binding proteins, where RalA, Rab3A, and Rab5A are the major GTP-binding proteins. Guanosine Triphosphate 102-105 RAS like proto-oncogene A Homo sapiens 65-69 10364219-1 1999 The Ral effector protein RLIP76 (also called RIP/RalBP1) binds to Ral.GTP via a region that shares no sequence homology with the Ras-binding domains of the Ser/Thr kinase c-Raf-1 and the Ral-specific guanine nucleotide exchange factors. Guanosine Triphosphate 70-73 RAS like proto-oncogene A Homo sapiens 4-7 10364219-1 1999 The Ral effector protein RLIP76 (also called RIP/RalBP1) binds to Ral.GTP via a region that shares no sequence homology with the Ras-binding domains of the Ser/Thr kinase c-Raf-1 and the Ral-specific guanine nucleotide exchange factors. Guanosine Triphosphate 70-73 RAS like proto-oncogene A Homo sapiens 49-52 10364219-1 1999 The Ral effector protein RLIP76 (also called RIP/RalBP1) binds to Ral.GTP via a region that shares no sequence homology with the Ras-binding domains of the Ser/Thr kinase c-Raf-1 and the Ral-specific guanine nucleotide exchange factors. Guanosine Triphosphate 70-73 RAS like proto-oncogene A Homo sapiens 49-52 9843482-4 1998 Between the three GTPase an intriguing interconnectivity exists, in that guanine nucleotide exchange factors for Ral associate with the GTP-bound form of both Ras and Rap1. Guanosine Triphosphate 18-21 RAS like proto-oncogene A Homo sapiens 113-116 10329639-0 1999 Ca2+/calmodulin stimulates GTP binding to the ras-related protein ral-A. Guanosine Triphosphate 27-30 RAS like proto-oncogene A Homo sapiens 46-71 10329639-4 1999 In this study we show the enhancement of GTP binding to Ral-A by Ca2+/calmodulin. Guanosine Triphosphate 41-44 RAS like proto-oncogene A Homo sapiens 56-61 9416833-1 1997 Ral GDP dissociation stimulator (RalGDS), a putative effector protein of Ras, stimulated the GDP/GTP exchange reaction of the post-tanslationally lipid-modified but not the unmodified form of Ral in response to epidermal growth factor in COS cells. Guanosine Triphosphate 97-100 RAS like proto-oncogene A Homo sapiens 0-3 9416833-6 1997 When Ral was incorporated with the GTP-bound form of Ras in the liposomes, RalGDS stimulated the dissociation of GDP from Ral, while the GDP-bound form of Ras did not affect the RalGDS action. Guanosine Triphosphate 35-38 RAS like proto-oncogene A Homo sapiens 5-8 9416833-6 1997 When Ral was incorporated with the GTP-bound form of Ras in the liposomes, RalGDS stimulated the dissociation of GDP from Ral, while the GDP-bound form of Ras did not affect the RalGDS action. Guanosine Triphosphate 35-38 RAS like proto-oncogene A Homo sapiens 75-78 9688545-3 1998 We found that RalA, another member of the small GTP-binding proteins, synergistically enhances the ARF1-dependent PLD activity with an EC50 of about 30 nM. Guanosine Triphosphate 48-51 RAS like proto-oncogene A Homo sapiens 14-18 9550702-2 1998 Recently, we and others found that three different Ral guanine nucleotide exchange factors (Ral GEFs) - Ral GDS, Rgl and Rlf - bind specifically to the GTP-bound form of several Ras-like GTPases [6-9]. Guanosine Triphosphate 152-155 RAS like proto-oncogene A Homo sapiens 51-54 9550702-2 1998 Recently, we and others found that three different Ral guanine nucleotide exchange factors (Ral GEFs) - Ral GDS, Rgl and Rlf - bind specifically to the GTP-bound form of several Ras-like GTPases [6-9]. Guanosine Triphosphate 152-155 RAS like proto-oncogene A Homo sapiens 92-95 9550702-2 1998 Recently, we and others found that three different Ral guanine nucleotide exchange factors (Ral GEFs) - Ral GDS, Rgl and Rlf - bind specifically to the GTP-bound form of several Ras-like GTPases [6-9]. Guanosine Triphosphate 152-155 RAS like proto-oncogene A Homo sapiens 92-95