PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 25169936-3 2014 APPROACH AND RESULTS: In addition to conventional markers, GTP-bound levels of Rap1, RhoA, RalA, Rac1, and Ras in platelets, which are implicated for platelet activation, were measured in patients without pulmonary hypertension (non-PH, n=15), patients with PAH (n=19), and patients with CTEPH (n=25). Guanosine Triphosphate 59-62 RAP1A, member of RAS oncogene family Homo sapiens 79-83 31545475-1 2019 Ras-associated protein 1A (Rap1A) is a member of the Ras subfamily of small GTP-binding proteins and is found to promote metastasis in several types of cancer. Guanosine Triphosphate 76-79 RAP1A, member of RAS oncogene family Homo sapiens 0-25 31545475-1 2019 Ras-associated protein 1A (Rap1A) is a member of the Ras subfamily of small GTP-binding proteins and is found to promote metastasis in several types of cancer. Guanosine Triphosphate 76-79 RAP1A, member of RAS oncogene family Homo sapiens 27-32 28740152-3 2017 This assay adapts a conventional ELISA format with immobilized RalGDS-RBD as a bait to selectively capture GTP-bound active Rap1. Guanosine Triphosphate 107-110 RAP1A, member of RAS oncogene family Homo sapiens 124-128 26277896-4 2017 Upon TLR engagement, GTP-bound Ras levels are increased and GTP-bound Rap1 is decreased, which can be reversed by EGTA-mediated removal of extracellular calcium. Guanosine Triphosphate 60-63 RAP1A, member of RAS oncogene family Homo sapiens 70-74 24704834-1 2015 SIPA1 (signal-induced proliferation-associated protein 1) is a GTPase activation protein that can catalyze the hydrolysis of Rap1 bound GTP to GDP. Guanosine Triphosphate 63-66 RAP1A, member of RAS oncogene family Homo sapiens 125-129 29337043-8 2018 Geranylgeranylated TAT-Rab27A-GTP-gamma-S promoted the exchange of GDP for GTP on Rab3 and Rap1 detected by far-immunofluorescence with Rab3-GTP and Rap1-GTP binding cassettes. Guanosine Triphosphate 30-33 RAP1A, member of RAS oncogene family Homo sapiens 91-95 29337043-8 2018 Geranylgeranylated TAT-Rab27A-GTP-gamma-S promoted the exchange of GDP for GTP on Rab3 and Rap1 detected by far-immunofluorescence with Rab3-GTP and Rap1-GTP binding cassettes. Guanosine Triphosphate 30-33 RAP1A, member of RAS oncogene family Homo sapiens 149-153 29158262-4 2018 The cAMP-dependent guanine nucleotide exchange factor (EPAC) exchange-protein directly activated by cAMP promotes exchange of GTP in the small GTPase Rap1. Guanosine Triphosphate 126-129 RAP1A, member of RAS oncogene family Homo sapiens 150-154 29158262-9 2018 Furthermore, GTP-bound Rap1 promoted tight junction assembly, and loss of Rap1B led to loss of junctional border organization. Guanosine Triphosphate 13-16 RAP1A, member of RAS oncogene family Homo sapiens 23-27 25856570-1 2015 RAP1 (RAS proximate 1), a small GTP-binding protein of the RAS superfamily, is a putative oncogene that is highly expressed in several malignant cell lines and types of cancers, including some types of squamous cell carcinoma. Guanosine Triphosphate 32-35 RAP1A, member of RAS oncogene family Homo sapiens 0-4 25856570-1 2015 RAP1 (RAS proximate 1), a small GTP-binding protein of the RAS superfamily, is a putative oncogene that is highly expressed in several malignant cell lines and types of cancers, including some types of squamous cell carcinoma. Guanosine Triphosphate 32-35 RAP1A, member of RAS oncogene family Homo sapiens 6-21 24352565-8 2014 In a purified recombinant protein system, PAO directly inhibited CalDAG-GEFI-stimulated GTP binding to Rap1. Guanosine Triphosphate 88-91 RAP1A, member of RAS oncogene family Homo sapiens 103-107 24287201-4 2014 Herein we present the crystal structure of an active, GTP-bound GTPase domain of Rap1 in complex with the Ras association (RA)-pleckstrin homology (PH) structural module of RIAM at 1.65 A. Guanosine Triphosphate 54-57 RAP1A, member of RAS oncogene family Homo sapiens 81-85 24349085-6 2013 Ca(2+)-activated wtTG2 cross-linked RAP1, GTP-GDP dissociation stimulator 1, an unusual guanine exchange factor acting on various small GTPases, to induce a yet uncharacterized signaling pathway that was able to promote the Ca(2+) release from the endoplasmic reticulum via both Ins3P and ryanodine sensitive receptors leading to a consequently enhanced mitochondrial Ca(2+)uptake. Guanosine Triphosphate 42-45 RAP1A, member of RAS oncogene family Homo sapiens 36-40 24470025-1 2014 Rap1 GTPases operate as molecular switches by cycling between a GDP-bound inactive state and a GTP-bound active state and regulate several cellular pathways in response to different stimuli. Guanosine Triphosphate 5-8 RAP1A, member of RAS oncogene family Homo sapiens 0-4 22614916-1 2012 Rap1GAP which regulates the GTP-GDP form switch of Rap1 is a member of the GTPase-activating protein (GAP) family and has recently received substantial attention. Guanosine Triphosphate 28-31 RAP1A, member of RAS oncogene family Homo sapiens 0-4 23946483-5 2013 One, it decreases the level of Rap1 activity as measured by GTP loading and the coupling of Rap1 to RapL, a Rap1 effector that couples Rap1 GTP loading to integrin activation. Guanosine Triphosphate 60-63 RAP1A, member of RAS oncogene family Homo sapiens 31-35 23946483-5 2013 One, it decreases the level of Rap1 activity as measured by GTP loading and the coupling of Rap1 to RapL, a Rap1 effector that couples Rap1 GTP loading to integrin activation. Guanosine Triphosphate 140-143 RAP1A, member of RAS oncogene family Homo sapiens 31-35 23946483-5 2013 One, it decreases the level of Rap1 activity as measured by GTP loading and the coupling of Rap1 to RapL, a Rap1 effector that couples Rap1 GTP loading to integrin activation. Guanosine Triphosphate 140-143 RAP1A, member of RAS oncogene family Homo sapiens 92-96 23946483-5 2013 One, it decreases the level of Rap1 activity as measured by GTP loading and the coupling of Rap1 to RapL, a Rap1 effector that couples Rap1 GTP loading to integrin activation. Guanosine Triphosphate 140-143 RAP1A, member of RAS oncogene family Homo sapiens 92-96 23946483-5 2013 One, it decreases the level of Rap1 activity as measured by GTP loading and the coupling of Rap1 to RapL, a Rap1 effector that couples Rap1 GTP loading to integrin activation. Guanosine Triphosphate 140-143 RAP1A, member of RAS oncogene family Homo sapiens 92-96 23946483-7 2013 These two actions, decreased GTP loading and decreased membrane localization, are related, as the translocation of Rap1-GTP into the cytoplasm is associated with its increased GTP hydrolysis and inactivation. Guanosine Triphosphate 29-32 RAP1A, member of RAS oncogene family Homo sapiens 115-119 23946483-7 2013 These two actions, decreased GTP loading and decreased membrane localization, are related, as the translocation of Rap1-GTP into the cytoplasm is associated with its increased GTP hydrolysis and inactivation. Guanosine Triphosphate 120-123 RAP1A, member of RAS oncogene family Homo sapiens 115-119 23097489-4 2012 On activation and chemotactic migration of neutrophils, Radil quickly translocates from the cytoplasm to the plasma membrane in a Rap1a-GTP-dependent manner. Guanosine Triphosphate 136-139 RAP1A, member of RAS oncogene family Homo sapiens 130-135 23804711-8 2013 Taken together, our findings indicate that chemokine unresponsiveness in CLL lymphocytes results from failure of Arf1/phospholipase D1-mediated translocation of Rap1 to the plasma membrane for GTP loading and may be a specific feature of anergy induced by DNA Ags. Guanosine Triphosphate 193-196 RAP1A, member of RAS oncogene family Homo sapiens 161-165 23652597-9 2013 Db cAMP increased both phosphorylated PKA and GTP-bound Rap1. Guanosine Triphosphate 46-49 RAP1A, member of RAS oncogene family Homo sapiens 56-60 21337364-7 2011 In addition, N(6)-benzoyl-cAMP (100 muM) increased Rap1 GTP expression, as did 8-pCPT; they were suppressed by Rp-cAMPS and GGTI-298. Guanosine Triphosphate 56-59 RAP1A, member of RAS oncogene family Homo sapiens 51-55 21750123-12 2011 However, the loading of GTP on Rap1 per se is not sufficient to promote activation of beta2 integrins. Guanosine Triphosphate 24-27 RAP1A, member of RAS oncogene family Homo sapiens 31-35 20709748-5 2010 We demonstrate that insertion of the dominant negative mutation to inhibit GDP/GTP exchange diminishes prenylation of Rap1A and RhoA, enhances prenylation of Rac1, and does not detectably alter prenylation of K-Ras. Guanosine Triphosphate 79-82 RAP1A, member of RAS oncogene family Homo sapiens 118-123 20413728-2 2010 CalDAG-GEFI plays a key role in the activation of beta1, beta2, and beta3 integrins in platelets and neutrophils by exchanging a GDP for a GTP on Rap1. Guanosine Triphosphate 139-142 RAP1A, member of RAS oncogene family Homo sapiens 146-150 19788733-7 2009 GTP-loading of Rap1 and Rap2 was evaluated via pull-down technique. Guanosine Triphosphate 0-3 RAP1A, member of RAS oncogene family Homo sapiens 15-19 20170508-7 2010 Indeed, all retrogene-encoded GTPases have an increased GTP/GDP binding ratio in vivo, indicating that their conformations resemble that of active GTP-bound Rap1. Guanosine Triphosphate 30-33 RAP1A, member of RAS oncogene family Homo sapiens 157-161 20170508-7 2010 Indeed, all retrogene-encoded GTPases have an increased GTP/GDP binding ratio in vivo, indicating that their conformations resemble that of active GTP-bound Rap1. Guanosine Triphosphate 56-59 RAP1A, member of RAS oncogene family Homo sapiens 157-161 20124489-2 2010 Rap1GAP, a Rap1 GTPase-activating protein, inhibits the RAS superfamily protein Rap1 by facilitating hydrolysis of GTP to GDP. Guanosine Triphosphate 16-19 RAP1A, member of RAS oncogene family Homo sapiens 0-4 20124489-2 2010 Rap1GAP, a Rap1 GTPase-activating protein, inhibits the RAS superfamily protein Rap1 by facilitating hydrolysis of GTP to GDP. Guanosine Triphosphate 16-19 RAP1A, member of RAS oncogene family Homo sapiens 11-15 19788733-15 2009 6-Bnz-cAMP and 8-pCPT-2"-O-Me-cAMP induced GTP-loading of Rap1, but not of Rap2. Guanosine Triphosphate 43-46 RAP1A, member of RAS oncogene family Homo sapiens 58-62 19433443-10 2009 In contrast, we present evidence consistent with a model in which the RasGRD of GAP1(IP4BP) functions to stabilize the switch II region of Rap1, allowing stabilization of the transition state during GTP hydrolysis initiated by the arginine finger. Guanosine Triphosphate 199-202 RAP1A, member of RAS oncogene family Homo sapiens 139-143 19086031-4 2009 ET-1 stimulation resulted in a significant increase of Pyk2 phosphorylation accompanied by GTP-loading of Rap1 and RhoA. Guanosine Triphosphate 91-94 RAP1A, member of RAS oncogene family Homo sapiens 106-110 19464664-3 2009 Rap1 was expressed in RSF, and GTP-bound active Rap1 (GTP-Rap1) was rapidly increased by PGE(2). Guanosine Triphosphate 31-34 RAP1A, member of RAS oncogene family Homo sapiens 48-52 19464664-3 2009 Rap1 was expressed in RSF, and GTP-bound active Rap1 (GTP-Rap1) was rapidly increased by PGE(2). Guanosine Triphosphate 31-34 RAP1A, member of RAS oncogene family Homo sapiens 54-62 18784646-1 2009 The guanine nucleotide exchange factor C3G, along with the CrkII adaptor protein, mediates GTP activation of the small GTPase proteins Rap1 and R-Ras, facilitating their activation of downstream signaling pathways, which had been found to be important in the pathogenesis of glomerulonephritis. Guanosine Triphosphate 91-94 RAP1A, member of RAS oncogene family Homo sapiens 135-139 18784646-4 2009 Activation of Rap1 was not affected by C3G; however, the basal level of GTP-bound R-Ras was decreased. Guanosine Triphosphate 72-75 RAP1A, member of RAS oncogene family Homo sapiens 14-18 16236484-12 2006 In conclusion, the data reveal a novel type of cross-talk between P2Y12 and IGF-I receptors that proceeds through Gbetagamma-, Ca2+-and PLD2-dependent activation of the Pyk2/Src pathway resulting in GTP-loading of Rap1 required for an increased PKB phosphorylation. Guanosine Triphosphate 199-202 RAP1A, member of RAS oncogene family Homo sapiens 214-218 18922916-6 2008 Furthermore, we show that this defect in Rap1 GTP-loading is at the level of the GTPase and is corrected in CLL cells cultured in the absence of exogenous stimuli, suggesting that the defect is the result of in vivo stimulation. Guanosine Triphosphate 46-49 RAP1A, member of RAS oncogene family Homo sapiens 41-45 17517894-5 2007 In vitro analysis demonstrates a direct interaction between Rap1 and IQGAP1, which is augmented by activation (GTP loading) of Rap1. Guanosine Triphosphate 111-114 RAP1A, member of RAS oncogene family Homo sapiens 60-64 17517894-5 2007 In vitro analysis demonstrates a direct interaction between Rap1 and IQGAP1, which is augmented by activation (GTP loading) of Rap1. Guanosine Triphosphate 111-114 RAP1A, member of RAS oncogene family Homo sapiens 127-131 16963453-1 2006 We found that engagement of beta2 integrins on human neutrophils increased the levels of GTP-bound Rap1 and Rap2. Guanosine Triphosphate 89-92 RAP1A, member of RAS oncogene family Homo sapiens 99-103 17300802-1 2007 Rap1 and Rap2 are the only small guanine nucleotide-binding proteins of the Ras superfamily that do not use glutamine for GTP hydrolysis. Guanosine Triphosphate 122-125 RAP1A, member of RAS oncogene family Homo sapiens 0-4 16213650-1 2006 The Rap family of small GTP-binding proteins is composed by four different members: Rap1A, Rap1B, Rap2A and Rap2B. Guanosine Triphosphate 24-27 RAP1A, member of RAS oncogene family Homo sapiens 84-89 16507992-15 2006 This activation requires the GTP-dependent association of Rap1 with B-Raf. Guanosine Triphosphate 29-32 RAP1A, member of RAS oncogene family Homo sapiens 58-62 14757755-4 2004 The PM association of GFP-Rap1 was dependent on GTP binding, and GFP-Rap1 was rapidly up-regulated on this compartment in response to mitogens, a process blocked by inhibitors of endosome recycling. Guanosine Triphosphate 48-51 RAP1A, member of RAS oncogene family Homo sapiens 26-30 16436672-4 2006 Using a pull-down assay, active GTP-bound rap1 was up-regulated in SCC compared to normal or immortalized keratinocytes. Guanosine Triphosphate 32-35 RAP1A, member of RAS oncogene family Homo sapiens 42-46 16172130-5 2005 Incubation of macrophages with forskolin also increased Epac1 and Rap1.GTP. Guanosine Triphosphate 71-74 RAP1A, member of RAS oncogene family Homo sapiens 66-70 16014623-3 2005 RA-RhoGAP has the RA and GAP domains and showed GAP activity specific for Rho, which was enhanced by the binding of the GTP-bound active form of Rap1 to the RA domain. Guanosine Triphosphate 120-123 RAP1A, member of RAS oncogene family Homo sapiens 145-149 15613546-5 2005 We found that agonists known to induce cAMP signaling promoted the GTP-bound, active state of Rap1 in SS RBCs. Guanosine Triphosphate 67-70 RAP1A, member of RAS oncogene family Homo sapiens 94-98 16170364-6 2006 c-Src then tyrosine-phosphorylated Vav2, one of the Rac-GDP/GTP exchange factors (GEFs), and induced activation of C3G, one of the Rap1-GEFs, through Crk adaptor protein, resulting in the activation of Rap1 locally at the cadherin-based cell-cell adhesion sites. Guanosine Triphosphate 60-63 RAP1A, member of RAS oncogene family Homo sapiens 131-135 16170364-6 2006 c-Src then tyrosine-phosphorylated Vav2, one of the Rac-GDP/GTP exchange factors (GEFs), and induced activation of C3G, one of the Rap1-GEFs, through Crk adaptor protein, resulting in the activation of Rap1 locally at the cadherin-based cell-cell adhesion sites. Guanosine Triphosphate 60-63 RAP1A, member of RAS oncogene family Homo sapiens 202-206 16757332-1 2006 Phospholipase C (PLC) epsilon is directly regulated by Ras and Rap1 small GTPases: Ras and Rap1, in their GTP-bound form, interact with the Ras/Rap1-associationg (RA) domain of PLCepsilon, thereby translocating PLCepsilon to the plasma membrane and the Golgi apparatus, respectively. Guanosine Triphosphate 74-77 RAP1A, member of RAS oncogene family Homo sapiens 63-67 16757332-1 2006 Phospholipase C (PLC) epsilon is directly regulated by Ras and Rap1 small GTPases: Ras and Rap1, in their GTP-bound form, interact with the Ras/Rap1-associationg (RA) domain of PLCepsilon, thereby translocating PLCepsilon to the plasma membrane and the Golgi apparatus, respectively. Guanosine Triphosphate 74-77 RAP1A, member of RAS oncogene family Homo sapiens 91-95 16757332-1 2006 Phospholipase C (PLC) epsilon is directly regulated by Ras and Rap1 small GTPases: Ras and Rap1, in their GTP-bound form, interact with the Ras/Rap1-associationg (RA) domain of PLCepsilon, thereby translocating PLCepsilon to the plasma membrane and the Golgi apparatus, respectively. Guanosine Triphosphate 74-77 RAP1A, member of RAS oncogene family Homo sapiens 91-95 16757333-5 2006 The founding member, Nore1A, binds preferentially through its RA domain to the GTP-charged forms of Ras, Rap-1, and several other Ras subfamily GTPases with high affinity. Guanosine Triphosphate 79-82 RAP1A, member of RAS oncogene family Homo sapiens 105-110 14757755-5 2004 A novel fluorescent probe for GTP-bound Rap1 revealed that this GTPase was transiently activated only on the PM of both fibroblasts and T cells. Guanosine Triphosphate 30-33 RAP1A, member of RAS oncogene family Homo sapiens 40-44 13679863-0 2003 Rap1A and rap1B ras-family proteins are prominently expressed in the nucleus of squamous carcinomas: nuclear translocation of GTP-bound active form. Guanosine Triphosphate 126-129 RAP1A, member of RAS oncogene family Homo sapiens 0-5 13679863-9 2003 This novel discovery that active, GTP-bound rap1 translocates to the nucleus makes it only the second of over 100 small GTP-binding proteins to be identified in the nucleus, and the striking prominence of rap1 expression in the nucleus of SCC cells suggests that activated rap1 plays a role in the malignant process. Guanosine Triphosphate 34-37 RAP1A, member of RAS oncogene family Homo sapiens 44-48 13679863-9 2003 This novel discovery that active, GTP-bound rap1 translocates to the nucleus makes it only the second of over 100 small GTP-binding proteins to be identified in the nucleus, and the striking prominence of rap1 expression in the nucleus of SCC cells suggests that activated rap1 plays a role in the malignant process. Guanosine Triphosphate 34-37 RAP1A, member of RAS oncogene family Homo sapiens 205-209 13679863-9 2003 This novel discovery that active, GTP-bound rap1 translocates to the nucleus makes it only the second of over 100 small GTP-binding proteins to be identified in the nucleus, and the striking prominence of rap1 expression in the nucleus of SCC cells suggests that activated rap1 plays a role in the malignant process. Guanosine Triphosphate 34-37 RAP1A, member of RAS oncogene family Homo sapiens 205-209 13679863-9 2003 This novel discovery that active, GTP-bound rap1 translocates to the nucleus makes it only the second of over 100 small GTP-binding proteins to be identified in the nucleus, and the striking prominence of rap1 expression in the nucleus of SCC cells suggests that activated rap1 plays a role in the malignant process. Guanosine Triphosphate 120-123 RAP1A, member of RAS oncogene family Homo sapiens 44-48 12446733-3 2003 Raf-1 is also thought to be recruited to the small G protein Rap1 upon GTP loading of Rap1. Guanosine Triphosphate 71-74 RAP1A, member of RAS oncogene family Homo sapiens 61-65 12508104-1 2003 Rap1 belongs to the Ras subgroup of small GTP-binding proteins. Guanosine Triphosphate 42-45 RAP1A, member of RAS oncogene family Homo sapiens 0-4 12446733-3 2003 Raf-1 is also thought to be recruited to the small G protein Rap1 upon GTP loading of Rap1. Guanosine Triphosphate 71-74 RAP1A, member of RAS oncogene family Homo sapiens 86-90 12469113-2 2003 Recently, guanine nucleotide exchange factors specific for the small GTP-binding proteins Rap1 and Rap2 (Epacs) were described, which are also activated directly by cAMP. Guanosine Triphosphate 69-72 RAP1A, member of RAS oncogene family Homo sapiens 90-94 10934204-10 2000 By searching for in vitro interaction with Ras-GTP proteins, PDZ-GEF specifically bound to Rap1A- and Rap2B-GTP, whereas MR-GEF bound to M-Ras-GTP. Guanosine Triphosphate 47-50 RAP1A, member of RAS oncogene family Homo sapiens 91-96 12186953-6 2002 Dictyostelium Rap1 formed a complex with the Ras-binding domain of RalGDS only when it was in a GTP-bound state. Guanosine Triphosphate 96-99 RAP1A, member of RAS oncogene family Homo sapiens 14-18 12171996-6 2002 These findings indicate that available levels of GTP-bound Rap1 are required for the direct activation of LFA-1 and VLA-4. Guanosine Triphosphate 49-52 RAP1A, member of RAS oncogene family Homo sapiens 59-63 11788480-8 2002 These findings reveal a novel mechanism for elevating cytosolic Ca(2+) by cAMP, possibly via GTP-Rap1. Guanosine Triphosphate 93-96 RAP1A, member of RAS oncogene family Homo sapiens 97-101 11266512-3 2001 Elevated cAMP was also found to stimulate activation of the GTP-binding protein, Rap1. Guanosine Triphosphate 60-63 RAP1A, member of RAS oncogene family Homo sapiens 81-85 11022048-4 2001 The Ras-associating domain of PLCepsilon specifically binds to the GTP-bound forms of Ha-Ras and Rap1A. Guanosine Triphosphate 67-70 RAP1A, member of RAS oncogene family Homo sapiens 97-102 10835426-2 2000 This novel GEF, referred to as CalDAG-GEFIII, increased the GTP/GDP ratio of Ha-Ras, R-Ras, and Rap1 in 293T cells. Guanosine Triphosphate 60-63 RAP1A, member of RAS oncogene family Homo sapiens 96-100 10982853-5 2000 In addition, we show that Sin- and Cas-induced Src signaling, as assayed by transcriptional activation, is exclusively mediated through a pathway that involves the adapter Crk and the GTP-binding protein Rap1. Guanosine Triphosphate 184-187 RAP1A, member of RAS oncogene family Homo sapiens 204-208 10962559-4 2000 We have now developed an enzyme-based method for assessing Rap 1 activation which is quantitative and provides a measurement of the per cent of Rap molecules in the active GTP-bound state. Guanosine Triphosphate 172-175 RAP1A, member of RAS oncogene family Homo sapiens 59-64 10753869-9 2000 Transfection of nonmutated Gab1 led to a strong increase of Rap1.GTP in the absence of HGF. Guanosine Triphosphate 65-68 RAP1A, member of RAS oncogene family Homo sapiens 60-64 10913189-5 2000 The half-life of GTP-Rap2 was significantly longer than that of GTP-Rap1 in 293T cells, indicating that low sensitivity to GAPs caused a high GTP/GDP ratio on Rap2. Guanosine Triphosphate 64-67 RAP1A, member of RAS oncogene family Homo sapiens 68-72 10913189-5 2000 The half-life of GTP-Rap2 was significantly longer than that of GTP-Rap1 in 293T cells, indicating that low sensitivity to GAPs caused a high GTP/GDP ratio on Rap2. Guanosine Triphosphate 64-67 RAP1A, member of RAS oncogene family Homo sapiens 68-72 10861233-2 2000 Here we report that LDL activates the small GTPases Rap1 and Ral but not Ras, as assessed by specific precipitation of the GTP-bound enzymes. Guanosine Triphosphate 44-47 RAP1A, member of RAS oncogene family Homo sapiens 52-56 10593970-3 1999 One of the molecules uncovered in this screen was Rap1GAP, a previously identified protein that specifically stimulates GTP hydrolytic activity of the monomeric G protein Rap1 and thus is believed to function as a down-regulator of Rap1 signaling. Guanosine Triphosphate 120-123 RAP1A, member of RAS oncogene family Homo sapiens 50-54 10608844-5 1999 Hs-RA-GEF exhibited not only a GTP-dependent binding activity to Rap1A at its RA domain but also an activity to stimulate GDP/GTP exchange of Rap1A both in vitro and in vivo at the segment containing its REM and GEF domains. Guanosine Triphosphate 31-34 RAP1A, member of RAS oncogene family Homo sapiens 65-70 10608844-5 1999 Hs-RA-GEF exhibited not only a GTP-dependent binding activity to Rap1A at its RA domain but also an activity to stimulate GDP/GTP exchange of Rap1A both in vitro and in vivo at the segment containing its REM and GEF domains. Guanosine Triphosphate 126-129 RAP1A, member of RAS oncogene family Homo sapiens 142-147 10608844-7 1999 On the other hand, Ce-RA-GEF associated with and stimulated GDP/GTP exchange of both Ras and Rap1A. Guanosine Triphosphate 64-67 RAP1A, member of RAS oncogene family Homo sapiens 93-98 10593970-3 1999 One of the molecules uncovered in this screen was Rap1GAP, a previously identified protein that specifically stimulates GTP hydrolytic activity of the monomeric G protein Rap1 and thus is believed to function as a down-regulator of Rap1 signaling. Guanosine Triphosphate 120-123 RAP1A, member of RAS oncogene family Homo sapiens 171-175 9882515-0 1999 RAP1A GTP/GDP cycles determine the intracellular location of the late endocytic compartments and contribute to myogenic differentiation. Guanosine Triphosphate 6-9 RAP1A, member of RAS oncogene family Homo sapiens 0-5 9882515-3 1999 By monitoring the nucleotide-bound form of RAP1A protein, we established that the various protein localizations were related to the GTP/GDP-bound state. Guanosine Triphosphate 132-135 RAP1A, member of RAS oncogene family Homo sapiens 43-48 9882515-8 1999 Whereas ectopic expression of wild-type or GTP-bound RAP1A proteins inhibited myogenic differentiation, the GDP-bound mutant favors myotubes formation. Guanosine Triphosphate 43-46 RAP1A, member of RAS oncogene family Homo sapiens 53-58 9853756-3 1998 At least three different second messengers, namely diacylglycerol, calcium and cyclic AMP, are able to activate Rap1 by promoting its release of the guanine nucleotide GDP and its binding to GTP. Guanosine Triphosphate 191-194 RAP1A, member of RAS oncogene family Homo sapiens 112-116 10318861-6 1999 Deletion of the amino terminus of C3G to amino acid 579 significantly reduced the Crk-dependent tyrosine phosphorylation of C3G and increased GTP-bound Rap1 irrespective of the presence of CrkI. Guanosine Triphosphate 142-145 RAP1A, member of RAS oncogene family Homo sapiens 152-156 9478939-4 1998 Utilizing a recently developed technique for detection of endogenous, GTP-bound Rap1, we have found that Rap1, but not Rap2, is transiently activated following T cell receptor stimulation of normal human T lymphocytes. Guanosine Triphosphate 70-73 RAP1A, member of RAS oncogene family Homo sapiens 80-84 9843482-4 1998 Between the three GTPase an intriguing interconnectivity exists, in that guanine nucleotide exchange factors for Ral associate with the GTP-bound form of both Ras and Rap1. Guanosine Triphosphate 18-21 RAP1A, member of RAS oncogene family Homo sapiens 167-171 9564038-1 1998 Insulin stimulation of Chinese hamster ovary cells expressing the human insulin receptor resulted in a time-dependent decrease in the amount of GTP bound to Rap1. Guanosine Triphosphate 144-147 RAP1A, member of RAS oncogene family Homo sapiens 157-161 9478939-4 1998 Utilizing a recently developed technique for detection of endogenous, GTP-bound Rap1, we have found that Rap1, but not Rap2, is transiently activated following T cell receptor stimulation of normal human T lymphocytes. Guanosine Triphosphate 70-73 RAP1A, member of RAS oncogene family Homo sapiens 105-109 9102473-12 1997 By using Rap1A and Ran, we show that the acceleration of the GTPase by Mn2+ appears to be a general phenomenon of GTP-binding proteins. Guanosine Triphosphate 61-64 RAP1A, member of RAS oncogene family Homo sapiens 9-14 9311917-3 1997 The adapter protein CrkL was associated with both phosphorylated Cbl and the guanidine nucleotide-releasing factor C3G, which catalyzes guanosine triphosphate (GTP) exchange on Rap1. Guanosine Triphosphate 136-158 RAP1A, member of RAS oncogene family Homo sapiens 177-181 9311917-3 1997 The adapter protein CrkL was associated with both phosphorylated Cbl and the guanidine nucleotide-releasing factor C3G, which catalyzes guanosine triphosphate (GTP) exchange on Rap1. Guanosine Triphosphate 160-163 RAP1A, member of RAS oncogene family Homo sapiens 177-181 9311917-4 1997 Active Rap1 (GTP-bound form) was present in anergic cells. Guanosine Triphosphate 13-16 RAP1A, member of RAS oncogene family Homo sapiens 7-11 8034626-1 1994 Rap1A is a GTP-binding protein of the Ras superfamily that is highly abundant in phagocyte membranes. Guanosine Triphosphate 11-14 RAP1A, member of RAS oncogene family Homo sapiens 0-5 9145335-4 1996 Small GTP-binding proteins Rac1, Rap1, and RhoA, which are reported to be implicated in O2- generation or PLD activation, were already expressed in undifferentiated HL60 cells and their significant changes were not detected during differentiation. Guanosine Triphosphate 6-9 RAP1A, member of RAS oncogene family Homo sapiens 33-37 8838585-1 1996 rap-1A, an anti-oncogene-encoded protein, is a ras-p21-like protein whose sequence is over 80% homologous to p21 and which interacts with the same intracellular target proteins and is activated by the same mechanisms as p21, e.g., by binding GTP in place of GDP. Guanosine Triphosphate 242-245 RAP1A, member of RAS oncogene family Homo sapiens 0-6 8838585-5 1996 We have constructed the three-dimensional structure of rap-1A bound to GTP by using the energy-minimized three-dimensional structure of ras-p21 as the basis for the modeling using a stepwise procedure in which identical and homologous amino acid residues in rap-1A are assumed to adopt the same conformation as the corresponding residues in p21. Guanosine Triphosphate 71-74 RAP1A, member of RAS oncogene family Homo sapiens 55-61 8838585-5 1996 We have constructed the three-dimensional structure of rap-1A bound to GTP by using the energy-minimized three-dimensional structure of ras-p21 as the basis for the modeling using a stepwise procedure in which identical and homologous amino acid residues in rap-1A are assumed to adopt the same conformation as the corresponding residues in p21. Guanosine Triphosphate 71-74 RAP1A, member of RAS oncogene family Homo sapiens 258-264 7833480-12 1995 production by the differentiated cell line expressing GTPase-defective V12 Rap1a was also significantly inhibited, a finding that is consistent with a requirement for cycling between guanosine diphosphate- and GTP-bound forms of Rap1a for continuous NADPH oxidase activation in intact neutrophils. Guanosine Triphosphate 54-57 RAP1A, member of RAS oncogene family Homo sapiens 75-80 7833480-12 1995 production by the differentiated cell line expressing GTPase-defective V12 Rap1a was also significantly inhibited, a finding that is consistent with a requirement for cycling between guanosine diphosphate- and GTP-bound forms of Rap1a for continuous NADPH oxidase activation in intact neutrophils. Guanosine Triphosphate 54-57 RAP1A, member of RAS oncogene family Homo sapiens 229-234 7803284-0 1994 Ultrastructural localization of the small GTP-binding protein Rap1 in human platelets and megakaryocytes. Guanosine Triphosphate 42-45 RAP1A, member of RAS oncogene family Homo sapiens 62-66 9029146-2 1997 We have developed a novel assay to monitor the active, GTP-bound form of Rap1 based on the differential affinity of Rap1GTP and Rap1GDP for the Rap binding domain of RalGDS (RBD). Guanosine Triphosphate 55-58 RAP1A, member of RAS oncogene family Homo sapiens 73-77 8900189-1 1996 Smg GDS is a regulator having two activities on a group of small G proteins including the Rho and Rap1 family members and Ki-Ras; one is to stimulate their GDP/GTP exchange reactions, and the other is to inhibit their interactions with membranes. Guanosine Triphosphate 160-163 RAP1A, member of RAS oncogene family Homo sapiens 98-102 8832375-1 1996 rap-1A is a membrane-bound G-protein in the ras superfamily that, like the ras-p21 protein, is activated by binding GTP in place of GDP. Guanosine Triphosphate 116-119 RAP1A, member of RAS oncogene family Homo sapiens 0-6 8636102-0 1996 Differential interaction of the ras family GTP-binding proteins H-Ras, Rap1A, and R-Ras with the putative effector molecules Raf kinase and Ral-guanine nucleotide exchange factor. Guanosine Triphosphate 43-46 RAP1A, member of RAS oncogene family Homo sapiens 71-76 7498475-2 1995 rap1 p21 is a small GTP-binding protein which has the same amino acid sequence as the effector loop of ras p21. Guanosine Triphosphate 20-23 RAP1A, member of RAS oncogene family Homo sapiens 0-4 7498475-4 1995 The GTP-bound form of rap1 p21 interacted with RGL as well as did ras p21. Guanosine Triphosphate 4-7 RAP1A, member of RAS oncogene family Homo sapiens 22-26 7479768-5 1995 The Dbl-homology domains of Bcr and Abr were active in stimulating GTP binding to CDC42Hs, RhoA, Rac1, and Rac2 (rank order, CDC42Hs > RhoA > Rac1 = Rac2) but were inactive toward Rap1A and Ha-Ras. Guanosine Triphosphate 67-70 RAP1A, member of RAS oncogene family Homo sapiens 186-191 7791872-1 1995 The X-ray crystal structure of the complex between the Ras-related protein Rap1A in the GTP-analogue (GppNHp) form and the Ras-binding domain (RBD) of the Ras effector molecule c-Raf1, a Ser/Thr-specific protein kinase, has been solved to a resolution of 2.2 A. Guanosine Triphosphate 88-91 RAP1A, member of RAS oncogene family Homo sapiens 75-80 7737967-1 1995 Rap1 proteins belong to the Ras superfamily of small molecular weight GTP-binding proteins. Guanosine Triphosphate 70-73 RAP1A, member of RAS oncogene family Homo sapiens 0-4 8524135-2 1995 In addition, the association of the small GTP-binding protein Rap1A with cytochrome b-558 was discovered through the analysis of purified cytochrome b. Guanosine Triphosphate 42-45 RAP1A, member of RAS oncogene family Homo sapiens 62-67 8524135-4 1995 The ability to purify cytochrome b and cytochrome b-Rap1A complexes will also allow further analysis of the structure of this novel plasma membrane redox protein and the role of its association with low molecular weight GTP-binding proteins in the structure and regulation of the phagocyte NADPH oxidase. Guanosine Triphosphate 220-223 RAP1A, member of RAS oncogene family Homo sapiens 52-57 7980444-4 1994 SmgGDS was able to stimulate the incorporation of guanosine 5"-[gamma-thio]-triphosphate GTP[gamma S] into the RhoA, Rac2, Rac1, Rap1A and CDC42Hs GTP-binding proteins, but the activity was greatest toward RhoA and Rac2. Guanosine Triphosphate 89-92 RAP1A, member of RAS oncogene family Homo sapiens 129-134 8034626-3 1994 Therefore, we have stably transfected human Epstein-Barr virus-transformed B lymphocytes that possess an activable NADPH oxidase complex with cDNAs for mutants of Rap1A "locked" in a GTP-bound (63E) and GDP-bound (17N) state. Guanosine Triphosphate 183-186 RAP1A, member of RAS oncogene family Homo sapiens 163-168 8034626-9 1994 Furthermore, the inhibitory effect of both GTP- as well as GDP-bound mutants indicates that Rap1A functions in a dynamic cycle as opposed to a unidirectional pathway, as is the case for the other NADPH oxidase regulatory GTP-binding protein, Rac. Guanosine Triphosphate 43-46 RAP1A, member of RAS oncogene family Homo sapiens 92-97 8034626-9 1994 Furthermore, the inhibitory effect of both GTP- as well as GDP-bound mutants indicates that Rap1A functions in a dynamic cycle as opposed to a unidirectional pathway, as is the case for the other NADPH oxidase regulatory GTP-binding protein, Rac. Guanosine Triphosphate 221-224 RAP1A, member of RAS oncogene family Homo sapiens 92-97 8216264-1 1993 The low molecular weight G protein Rap1A constitutes a major fraction of the GTP-binding proteins found in the plasma membrane and specific granules of the neutrophil; however, the function of Rap1A in neutrophil responses remains unclear. Guanosine Triphosphate 77-80 RAP1A, member of RAS oncogene family Homo sapiens 35-40 8048970-1 1994 Rap1GAP (for Rap1 GTPase Activating Protein) is an 89 kD protein that highly stimulates the intrinsic GTPase activity of the small GTP binding protein Rap1. Guanosine Triphosphate 18-21 RAP1A, member of RAS oncogene family Homo sapiens 0-4 35294066-7 2022 Pulldown of active GEFs from HDMECs depleted of ArhGEF12 and treated with TNF show decreased GTP-bound Rap1A after four hours but increased GTP-bound RhoA after 12 h. In cell-free assays, ArhGEF12 immunoprecipitated from HDMECs is able to activate both Rap1A and RhoA, but not act on Rap2A-C, RhoB-C, or even Rap1B which shares 95% sequence identity with Rap1A. Guanosine Triphosphate 93-96 RAP1A, member of RAS oncogene family Homo sapiens 103-108 1730690-1 1992 Ras and Rap1 proteins are related GTP-dependent signal transducers which require Gly-12, the effector domain (residues 32-40), and Ala-59 for stimulation of their GTPase activities by GAP1 and GAP3, respectively. Guanosine Triphosphate 34-37 RAP1A, member of RAS oncogene family Homo sapiens 8-12 1310590-5 1992 The identity between the cAMP phosphoprotein and the rap1 GTP-binding protein was further examined by studying the functional effect of GTP on plasma membrane Ca2+ transport. Guanosine Triphosphate 58-61 RAP1A, member of RAS oncogene family Homo sapiens 53-57 1773804-3 1991 The K-rev-1 cDNA has the capacity to encode a protein with a calculated molecular weight of 21,000, having strong structural similarity to ras proteins (approximately 50% homology), especially in their guanosine triphosphate/guanosine diphosphate-binding, effector-binding, and membrane-attachment domains. Guanosine Triphosphate 202-224 RAP1A, member of RAS oncogene family Homo sapiens 4-11 2164710-6 1990 Binding of GAP to Rap1A-p21 was strictly guanosine triphosphate (GTP)-dependent. Guanosine Triphosphate 41-63 RAP1A, member of RAS oncogene family Homo sapiens 18-23 2110451-4 1990 The relative affinities of GTP and GDP for the binding site on rap1 were modulated by the presence of Mg2+, with a preferential affinity (approx. Guanosine Triphosphate 27-30 RAP1A, member of RAS oncogene family Homo sapiens 63-67 2110451-8 1990 Furthermore, the ability of a cytosolic rap1 GTPase-activating protein to stimulate neutrophil rap1 GTP hydrolysis was not modified by phosphorylation. Guanosine Triphosphate 45-48 RAP1A, member of RAS oncogene family Homo sapiens 40-44 2110451-8 1990 Furthermore, the ability of a cytosolic rap1 GTPase-activating protein to stimulate neutrophil rap1 GTP hydrolysis was not modified by phosphorylation. Guanosine Triphosphate 45-48 RAP1A, member of RAS oncogene family Homo sapiens 95-99 1763330-1 1991 Rap1A is a low molecular weight guanosine triphosphate (GTP)-binding protein in human neutrophil membranes whose cellular function is unknown. Guanosine Triphosphate 32-54 RAP1A, member of RAS oncogene family Homo sapiens 0-5 1763330-1 1991 Rap1A is a low molecular weight guanosine triphosphate (GTP)-binding protein in human neutrophil membranes whose cellular function is unknown. Guanosine Triphosphate 56-59 RAP1A, member of RAS oncogene family Homo sapiens 0-5 1939245-1 1991 Rap1A is a Ras-related GTP binding protein which has an amino acid sequence identical to that of Ras in the putative "effector" domain (amino acids 32-40). Guanosine Triphosphate 23-26 RAP1A, member of RAS oncogene family Homo sapiens 0-5 1939245-5 1991 We observed that both Rap1A-GDP and Rap1A-guanosine 5"-3-O-(thio)triphosphate (GTP gamma S) were able to block the inhibitory effect of Ras-GAP upon channel activation. Guanosine Triphosphate 79-82 RAP1A, member of RAS oncogene family Homo sapiens 22-27 1939245-5 1991 We observed that both Rap1A-GDP and Rap1A-guanosine 5"-3-O-(thio)triphosphate (GTP gamma S) were able to block the inhibitory effect of Ras-GAP upon channel activation. Guanosine Triphosphate 79-82 RAP1A, member of RAS oncogene family Homo sapiens 36-41 1939245-7 1991 A Rap1A Thr35----Ala mutation which bound GTP gamma S did not prevent K+ channel inhibition by Ras-GAP, suggesting that the antagonism by wild type Rap1A involves an interaction with GAP in the effector domain. Guanosine Triphosphate 42-45 RAP1A, member of RAS oncogene family Homo sapiens 2-7 1939245-8 1991 The effectiveness of Rap1A to inhibit Ras-GAP is dependent upon the amount of Ras-GAP present in the assay and can also be overcome by the addition of GTP-bound N-Ras (GC-43), suggesting a competitive mechanism is operative. Guanosine Triphosphate 151-154 RAP1A, member of RAS oncogene family Homo sapiens 21-26 1828804-2 1991 Both Ras and Rap1A proteins are G proteins and appear to serve as signal transducers only in the GTP-bound form. Guanosine Triphosphate 97-100 RAP1A, member of RAS oncogene family Homo sapiens 13-18 1902091-1 1991 The products of rap genes (rap1A, rap1B and rap2) are small molecular weight GTP-binding proteins that share approximately 50% homology with ras-p21s. Guanosine Triphosphate 77-80 RAP1A, member of RAS oncogene family Homo sapiens 27-32 1900291-2 1991 A "rap 1-common" antiserum, prepared against an 18-amino acid segment of the rap 1a protein near the proposed GTP-binding region, reacted with both rap 1a and rap 1b recombinant proteins expressed in Escherichia coli and with two low molecular weight GTP-binding proteins of 22 and 24 kDa in unstimulated human platelets. Guanosine Triphosphate 110-113 RAP1A, member of RAS oncogene family Homo sapiens 3-8 1900291-2 1991 A "rap 1-common" antiserum, prepared against an 18-amino acid segment of the rap 1a protein near the proposed GTP-binding region, reacted with both rap 1a and rap 1b recombinant proteins expressed in Escherichia coli and with two low molecular weight GTP-binding proteins of 22 and 24 kDa in unstimulated human platelets. Guanosine Triphosphate 110-113 RAP1A, member of RAS oncogene family Homo sapiens 77-83 1900291-2 1991 A "rap 1-common" antiserum, prepared against an 18-amino acid segment of the rap 1a protein near the proposed GTP-binding region, reacted with both rap 1a and rap 1b recombinant proteins expressed in Escherichia coli and with two low molecular weight GTP-binding proteins of 22 and 24 kDa in unstimulated human platelets. Guanosine Triphosphate 110-113 RAP1A, member of RAS oncogene family Homo sapiens 148-154 1900291-2 1991 A "rap 1-common" antiserum, prepared against an 18-amino acid segment of the rap 1a protein near the proposed GTP-binding region, reacted with both rap 1a and rap 1b recombinant proteins expressed in Escherichia coli and with two low molecular weight GTP-binding proteins of 22 and 24 kDa in unstimulated human platelets. Guanosine Triphosphate 251-254 RAP1A, member of RAS oncogene family Homo sapiens 3-8 1900291-2 1991 A "rap 1-common" antiserum, prepared against an 18-amino acid segment of the rap 1a protein near the proposed GTP-binding region, reacted with both rap 1a and rap 1b recombinant proteins expressed in Escherichia coli and with two low molecular weight GTP-binding proteins of 22 and 24 kDa in unstimulated human platelets. Guanosine Triphosphate 251-254 RAP1A, member of RAS oncogene family Homo sapiens 77-83 1900291-2 1991 A "rap 1-common" antiserum, prepared against an 18-amino acid segment of the rap 1a protein near the proposed GTP-binding region, reacted with both rap 1a and rap 1b recombinant proteins expressed in Escherichia coli and with two low molecular weight GTP-binding proteins of 22 and 24 kDa in unstimulated human platelets. Guanosine Triphosphate 251-254 RAP1A, member of RAS oncogene family Homo sapiens 148-154 2164710-6 1990 Binding of GAP to Rap1A-p21 was strictly guanosine triphosphate (GTP)-dependent. Guanosine Triphosphate 65-68 RAP1A, member of RAS oncogene family Homo sapiens 18-23 2160589-3 1990 Recombinant wild-type Rap1A bound GTP gamma S, GTP, and GDP with affinities similar to those observed for neutrophil Rap1 protein. Guanosine Triphosphate 34-37 RAP1A, member of RAS oncogene family Homo sapiens 22-27 2160589-3 1990 Recombinant wild-type Rap1A bound GTP gamma S, GTP, and GDP with affinities similar to those observed for neutrophil Rap1 protein. Guanosine Triphosphate 34-37 RAP1A, member of RAS oncogene family Homo sapiens 22-26 2160589-3 1990 Recombinant wild-type Rap1A bound GTP gamma S, GTP, and GDP with affinities similar to those observed for neutrophil Rap1 protein. Guanosine Triphosphate 47-50 RAP1A, member of RAS oncogene family Homo sapiens 22-27 2160589-3 1990 Recombinant wild-type Rap1A bound GTP gamma S, GTP, and GDP with affinities similar to those observed for neutrophil Rap1 protein. Guanosine Triphosphate 47-50 RAP1A, member of RAS oncogene family Homo sapiens 22-26 2160589-4 1990 The rate of exchange of GTP by Rap1 without Mg2+ was much slower than that by Ras. Guanosine Triphosphate 24-27 RAP1A, member of RAS oncogene family Homo sapiens 31-35 34603379-6 2021 Based on the resolved structure of Rap1-Rap1GAP complex, homology modeling implies that the Lys 624 residue is structurally homologous to the Lys 194 of Rap1GAP, a highly conserved lysine residue that is involved in the interface between Rap1 and Rap1GAP and critical for the affinity to Rap GTP. Guanosine Triphosphate 292-295 RAP1A, member of RAS oncogene family Homo sapiens 35-39 34603379-6 2021 Based on the resolved structure of Rap1-Rap1GAP complex, homology modeling implies that the Lys 624 residue is structurally homologous to the Lys 194 of Rap1GAP, a highly conserved lysine residue that is involved in the interface between Rap1 and Rap1GAP and critical for the affinity to Rap GTP. Guanosine Triphosphate 292-295 RAP1A, member of RAS oncogene family Homo sapiens 238-242 2507536-2 1989 We have purified, characterized, and identified two GTP-binding proteins with Mr of 25,000 (c25KG) and 21,000 (c21KG) from the cytosol fraction of human platelets. Guanosine Triphosphate 52-55 RAP1A, member of RAS oncogene family Homo sapiens 111-116 35365657-3 2022 Chemokines, cytokines and interleukins stimulate PI3Kgamma-mediated Rap1 activation, leading to conformational changes in integrin alpha4beta1 that promote myeloid cell extravasation and tumor inflammation Here we show that PI3Kgamma activates a high molecular weight form of myosin light chain kinase, MLCK210, that promotes myosin-dependent Rap1 GTP loading, leading to integrin alpha4beta1 activation. Guanosine Triphosphate 348-351 RAP1A, member of RAS oncogene family Homo sapiens 68-72 33872697-11 2021 Rap1 expression enhanced S6K1 phosphorylation and glucagon addition to culture medium promoted Rap1-GTP loading. Guanosine Triphosphate 100-103 RAP1A, member of RAS oncogene family Homo sapiens 0-4 2540745-7 1989 On the other hand, the most abundant GTP-binding protein with Mr of 21,000 (c21KG) in human platelet cytosol, which was identified as a transformation suppressor gene product (rap 1 protein, smg p21 and Krev-1 protein), was not phosphorylated by A-kinase under the same condition. Guanosine Triphosphate 37-40 RAP1A, member of RAS oncogene family Homo sapiens 76-81 2540745-7 1989 On the other hand, the most abundant GTP-binding protein with Mr of 21,000 (c21KG) in human platelet cytosol, which was identified as a transformation suppressor gene product (rap 1 protein, smg p21 and Krev-1 protein), was not phosphorylated by A-kinase under the same condition. Guanosine Triphosphate 37-40 RAP1A, member of RAS oncogene family Homo sapiens 176-181 2540745-7 1989 On the other hand, the most abundant GTP-binding protein with Mr of 21,000 (c21KG) in human platelet cytosol, which was identified as a transformation suppressor gene product (rap 1 protein, smg p21 and Krev-1 protein), was not phosphorylated by A-kinase under the same condition. Guanosine Triphosphate 37-40 RAP1A, member of RAS oncogene family Homo sapiens 203-209 33872697-11 2021 Rap1 expression enhanced S6K1 phosphorylation and glucagon addition to culture medium promoted Rap1-GTP loading. Guanosine Triphosphate 100-103 RAP1A, member of RAS oncogene family Homo sapiens 95-99 33391941-4 2020 RASGRP2 codes for the protein CalDAG-GEFI RAS (guanyl-releasing protein-2), a guanine nucleotide exchange factor for small guanosine triphosphate(GTP)ase Rap1. Guanosine Triphosphate 123-145 RAP1A, member of RAS oncogene family Homo sapiens 154-158 33391941-4 2020 RASGRP2 codes for the protein CalDAG-GEFI RAS (guanyl-releasing protein-2), a guanine nucleotide exchange factor for small guanosine triphosphate(GTP)ase Rap1. Guanosine Triphosphate 146-149 RAP1A, member of RAS oncogene family Homo sapiens 154-158 32539601-4 2020 Rap1 was in GTP-associated active state in both types of lEVs and Rap1-lEVs levels correlated with increased cardiovascular risks including stenosis. Guanosine Triphosphate 12-15 RAP1A, member of RAS oncogene family Homo sapiens 0-4 32764635-8 2020 These data indicate that the conversion of Rap1-GDP to GTP exerts two distinct effects stepwise on the conformation of alpha4beta7. Guanosine Triphosphate 55-58 RAP1A, member of RAS oncogene family Homo sapiens 43-47 31879129-3 2020 Here, we demonstrate that Shank3 NTD-ANK specifically binds to the guanosine triphosphate (GTP)-bound form of HRas and Rap1. Guanosine Triphosphate 67-89 RAP1A, member of RAS oncogene family Homo sapiens 119-123 31879129-3 2020 Here, we demonstrate that Shank3 NTD-ANK specifically binds to the guanosine triphosphate (GTP)-bound form of HRas and Rap1. Guanosine Triphosphate 91-94 RAP1A, member of RAS oncogene family Homo sapiens 119-123 31879129-5 2020 Binding of Shank3 to the GTP-loaded Rap1 slows down its GTP hydrolysis by SynGAP. Guanosine Triphosphate 25-28 RAP1A, member of RAS oncogene family Homo sapiens 36-40 31879129-5 2020 Binding of Shank3 to the GTP-loaded Rap1 slows down its GTP hydrolysis by SynGAP. Guanosine Triphosphate 56-59 RAP1A, member of RAS oncogene family Homo sapiens 36-40