PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 31478134-9 2020 Furthermore, cerebral I/R injury induced apparently increased expression of TLR4, RhoA-GTP, ROCK1/2, p-MLC2, and MMMP-2/9, which, however, could be remarkably alleviated by ANF intervention. Guanosine Triphosphate 87-90 ras homolog family member A Rattus norvegicus 82-86 34033116-14 2021 Moreover, fasudil also abolished the increased levels of GTP-RhoA/ROCK2, decreased levels of tight junction proteins and increased permeability of BCB caused by A2A R activation. Guanosine Triphosphate 57-60 ras homolog family member A Rattus norvegicus 61-65 33832886-6 2020 Moreover, fasudil could not only reduce the expression of total-cohoin, Rock, total-cofilin and total-MLC protein induced by optic nerve injury, but also reduce the relative expression of GTP-RhoA, p-cofilin and p-MLC protein. Guanosine Triphosphate 188-191 ras homolog family member A Rattus norvegicus 192-196 31920140-7 2020 Pull-down assays were performed to isolate the active GTP-bound form using the RhoA activation assay kits. Guanosine Triphosphate 54-57 ras homolog family member A Rattus norvegicus 79-83 31173168-5 2019 The level of RhoA-GTP in TGF-beta1-stimulated cells was significantly higher than that in control cells. Guanosine Triphosphate 18-21 ras homolog family member A Rattus norvegicus 13-17 31173168-8 2019 The active form of Rap1 (Rap1 V12) suppressed RhoA-GTP levels, whereas the dominant-negative form of Rap1 (Rap1 N17) augmented RhoA-GTP levels. Guanosine Triphosphate 51-54 ras homolog family member A Rattus norvegicus 46-50 31173168-8 2019 The active form of Rap1 (Rap1 V12) suppressed RhoA-GTP levels, whereas the dominant-negative form of Rap1 (Rap1 N17) augmented RhoA-GTP levels. Guanosine Triphosphate 132-135 ras homolog family member A Rattus norvegicus 127-131 27220778-0 2016 High glucose-induced fibronectin upregulation in cultured mesangial cells involves caveolin-1-dependent RhoA-GTP activation via Src kinase. Guanosine Triphosphate 109-112 ras homolog family member A Rattus norvegicus 104-108 26336927-3 2015 Here we further reported that RhoA, a small GTP-binding protein, interacts with and regulates TRPC1, thus enhancing SOC-mediated Ca(2+) entry (SOCE) and epithelial restitution after wounding. Guanosine Triphosphate 44-47 ras homolog family member A Rattus norvegicus 30-34 25354898-7 2015 Rac1, RhoA and RhoV are apoptosis-associated genes in the Rho guanosine triphosphate (GTP)ase family. Guanosine Triphosphate 62-84 ras homolog family member A Rattus norvegicus 6-10 25354898-7 2015 Rac1, RhoA and RhoV are apoptosis-associated genes in the Rho guanosine triphosphate (GTP)ase family. Guanosine Triphosphate 86-89 ras homolog family member A Rattus norvegicus 6-10 23237297-5 2013 Active GTP-bound RhoA levels were determined by ELISA. Guanosine Triphosphate 7-10 ras homolog family member A Rattus norvegicus 17-21 25447939-7 2015 Consistently, GTP-RhoA, a GEF-H1 substrate, was significantly upregulated after brain ischemia. Guanosine Triphosphate 14-17 ras homolog family member A Rattus norvegicus 18-22 24872317-7 2014 TGF-beta1 stimulated active RhoA-GTP and increased Rho kinase (ROCK). Guanosine Triphosphate 33-36 ras homolog family member A Rattus norvegicus 28-32 23237297-9 2013 RhoA-GTP was significantly reduced in lymphatics from the AAI group, compared to vehicle control. Guanosine Triphosphate 5-8 ras homolog family member A Rattus norvegicus 0-4 21454546-3 2011 We report a new mechanism of cAMP-mediated relaxation through Epac, a GTP exchange factor for the small GTPase Rap1 resulting in an increase in Rap1 activity and suppression of RhoA activity. Guanosine Triphosphate 70-73 ras homolog family member A Rattus norvegicus 177-181 23292809-7 2013 Endogenous RhoA and GTP-RhoA increased in response to CCh and the increase was reduced by pretreatment with atropine. Guanosine Triphosphate 20-23 ras homolog family member A Rattus norvegicus 24-28 22035369-3 2012 db-cAMP up- and down-regulates GTP-RhoA levels in PC12 cells in a time-dependent manner. Guanosine Triphosphate 31-34 ras homolog family member A Rattus norvegicus 35-39 23525454-4 2013 The cellular levels of GTP-bound Arf6 and GTP-bound RhoA were reduced by Fal-002-2. Guanosine Triphosphate 42-45 ras homolog family member A Rattus norvegicus 52-56 21756248-7 2012 Degradation analysis indicated that aspirin enhanced the protein degradation rate of RhoA and GDP-bound RhoA seemed to be more susceptible to aspirin-enhanced degradation compared with the GTP-bound form. Guanosine Triphosphate 189-192 ras homolog family member A Rattus norvegicus 85-89 21756248-7 2012 Degradation analysis indicated that aspirin enhanced the protein degradation rate of RhoA and GDP-bound RhoA seemed to be more susceptible to aspirin-enhanced degradation compared with the GTP-bound form. Guanosine Triphosphate 189-192 ras homolog family member A Rattus norvegicus 104-108 22004427-8 2012 After completion of the functional study, we measured the amount of guanosine triphosphate (GTP) RhoA by using a G-LISA RhoA Activation Assay, as well as the phosphorylation of 20 kDa myosin light chain (MLC20), myosin phosphatase-targeting subunit 1 (MYPT1) and protein kinase C-potentiated inhibitory protein for heterotrimeric myosin light-chain phosphatase of 17 kDa (CPI-17) by immunobloting. Guanosine Triphosphate 92-95 ras homolog family member A Rattus norvegicus 97-101 20354679-3 2010 We measured the activity of GTP-RhoA and Rho GTPase-activating protein (RhoGAP) and the phosphorylation level of the myosin light chain (MLC(20)), myosin phosphatase targeting subunit 1 (MYPT1), and PKC-potentiated inhibitory protein for heterotrimeric MLCP of 17 kDa (CPI17). Guanosine Triphosphate 28-31 ras homolog family member A Rattus norvegicus 32-36 20965238-11 2011 Furthermore, HMC05 decreased the amount of GTP RhoA activated by NaF. Guanosine Triphosphate 43-46 ras homolog family member A Rattus norvegicus 47-51 20965153-4 2011 CHS-111 reduced the cellular levels of GTP-bound RhoA, membrane recruitment of Rho-associated protein kinase 1 and the downstream myosin light chain 2 phosphorylation, and attenuated the interaction between phosphatidylinositol 4-phosphate 5-kinase (PIP5K) and Arf6, whereas it only slightly inhibited the guanine nucleotide exchange activity of human Dbs (DH/PH) protein and did not affect the arfaptin binding to Arf6. Guanosine Triphosphate 39-42 ras homolog family member A Rattus norvegicus 49-53 20578246-5 2010 GTP-bound RhoA levels were reduced in response to bFGF, which suggests that the inactivation of RhoA is essential to neurite outgrowth in response to bFGF. Guanosine Triphosphate 0-3 ras homolog family member A Rattus norvegicus 10-14 20578246-5 2010 GTP-bound RhoA levels were reduced in response to bFGF, which suggests that the inactivation of RhoA is essential to neurite outgrowth in response to bFGF. Guanosine Triphosphate 0-3 ras homolog family member A Rattus norvegicus 96-100 18577703-8 2008 Furthermore, both genistein and daidzein decreased the amount of GTP RhoA activated by either U46619 or KCl. Guanosine Triphosphate 65-68 ras homolog family member A Rattus norvegicus 69-73 21787615-3 2010 We measured the amount of GTP-RhoA as well as vascular tension. Guanosine Triphosphate 26-29 ras homolog family member A Rattus norvegicus 30-34 21787615-6 2010 NaF increased not only phosphorylation level of MYPT1(Thr855) and CPI17(Thr38), but also the amount of GTP-RhoA. Guanosine Triphosphate 103-106 ras homolog family member A Rattus norvegicus 107-111 19897743-6 2010 Finally, using a RhoA-GTP pull-down assay, we investigated the contribution of O(2)(-) to depolarization-induced RhoA activation. Guanosine Triphosphate 22-25 ras homolog family member A Rattus norvegicus 17-21 19819235-10 2010 Moreover, western blotting showed that Hemin treatment decreased the amount of the active form of GTP-RhoA but the total RhoA remained unchanged. Guanosine Triphosphate 98-101 ras homolog family member A Rattus norvegicus 102-106 20200473-3 2010 RhoA belongs to Ras-related small GTP-binding proteins, which regulate a variety of cellular processes, including cell morphology alteration, actin dynamics, and cell migration. Guanosine Triphosphate 34-37 ras homolog family member A Rattus norvegicus 0-4 20200473-4 2010 NGF suppressed GTP-RhoA levels after 12 h in PC12 cells and was consistently required for a long time to induce neurite outgrowth. Guanosine Triphosphate 15-18 ras homolog family member A Rattus norvegicus 19-23 19125588-1 2009 The GTP-binding protein RhoA regulates microfilament dynamics in many cell types and mediates the inhibition of axonal regeneration by myelin and chondroitin sulfate proteoglycans. Guanosine Triphosphate 4-7 ras homolog family member A Rattus norvegicus 24-28 18554585-8 2008 Activated GTP-bound RHOA, undetectable in intact ganglia, was dramatically upregulated in both neurons and axons after injury. Guanosine Triphosphate 10-13 ras homolog family member A Rattus norvegicus 20-24 18348285-4 2008 Down regulation of SmgGDS expression by siRNA transfection resulted in a decrease of RhoA-GTP levels, enhanced cell spreading, and loss of the characteristic elongated morphology of VSMC. Guanosine Triphosphate 90-93 ras homolog family member A Rattus norvegicus 85-89 18296496-1 2008 Recent studies revealed an importance of a monomeric GTP-binding protein, RhoA, in contraction of bronchial smooth muscle (BSM). Guanosine Triphosphate 53-56 ras homolog family member A Rattus norvegicus 74-78 18411229-1 2008 AIMS: Recent studies from our laboratory demonstrated that increased expression of the small GTP-binding protein RhoA and activation of the RhoA/rho kinase (ROCK) pathway play an important role in the contractile dysfunction associated with diabetic cardiomyopathy in hearts from streptozotocin (STZ)-induced diabetic rats. Guanosine Triphosphate 93-96 ras homolog family member A Rattus norvegicus 113-117 16792569-7 2006 RESULTS: In freshly isolated hepatocytes from ethanol-fed rats, the GTP-bound (active) forms of Rac and Cdc42 were significantly decreased compared with pair-fed control rats, while the GTP-bound form of RhoA was not significantly altered. Guanosine Triphosphate 186-189 ras homolog family member A Rattus norvegicus 204-208 16787950-7 2006 Furthermore, inhibition of PKCalpha could block the increase in the GTP levels of RhoA induced by clustering of syndecan-4 at the cell surface. Guanosine Triphosphate 68-71 ras homolog family member A Rattus norvegicus 82-86 16530523-4 2006 Activation of aortic RhoA was examined by pull down of guanosine triphosphate (GTP)-RhoA and membrane translocation of RhoA. Guanosine Triphosphate 55-77 ras homolog family member A Rattus norvegicus 21-25 16109481-3 2006 According to the molecular switch hypothesis of GTPase function GTP-loading of RhoA should be sufficient to activate its effectors uniformly. Guanosine Triphosphate 48-51 ras homolog family member A Rattus norvegicus 79-83 16109481-4 2006 However, when monitoring NGF-induced binding of GTP-RhoA to multiple targets, we noted differential interactions with its effectors. Guanosine Triphosphate 48-51 ras homolog family member A Rattus norvegicus 52-56 16109481-10 2006 Therefore, phosphorylation of serine(188) may serve as a novel secondary switch of RhoA capable of overriding GTP-binding-elicited effector activation to a subset of targets such as ROK, which interact with the C-terminus of RhoA. Guanosine Triphosphate 110-113 ras homolog family member A Rattus norvegicus 83-87 16109481-10 2006 Therefore, phosphorylation of serine(188) may serve as a novel secondary switch of RhoA capable of overriding GTP-binding-elicited effector activation to a subset of targets such as ROK, which interact with the C-terminus of RhoA. Guanosine Triphosphate 110-113 ras homolog family member A Rattus norvegicus 225-229 16448651-3 2006 To evaluate whether traumatic brain injury (TBI) results in changes in Rho activation in vulnerable regions of the brain, GTP-RhoA pull down assays were performed on rat cortical and hippocampal tissue homogenates obtained from 24 h to 3 days following lateral fluid percussion brain injury (FPI). Guanosine Triphosphate 122-125 ras homolog family member A Rattus norvegicus 126-130 16530523-4 2006 Activation of aortic RhoA was examined by pull down of guanosine triphosphate (GTP)-RhoA and membrane translocation of RhoA. Guanosine Triphosphate 55-77 ras homolog family member A Rattus norvegicus 84-88 16530523-4 2006 Activation of aortic RhoA was examined by pull down of guanosine triphosphate (GTP)-RhoA and membrane translocation of RhoA. Guanosine Triphosphate 55-77 ras homolog family member A Rattus norvegicus 84-88 16530523-4 2006 Activation of aortic RhoA was examined by pull down of guanosine triphosphate (GTP)-RhoA and membrane translocation of RhoA. Guanosine Triphosphate 79-82 ras homolog family member A Rattus norvegicus 21-25 16530523-4 2006 Activation of aortic RhoA was examined by pull down of guanosine triphosphate (GTP)-RhoA and membrane translocation of RhoA. Guanosine Triphosphate 79-82 ras homolog family member A Rattus norvegicus 84-88 16530523-4 2006 Activation of aortic RhoA was examined by pull down of guanosine triphosphate (GTP)-RhoA and membrane translocation of RhoA. Guanosine Triphosphate 79-82 ras homolog family member A Rattus norvegicus 84-88 16311049-2 2005 Rnd1, a novel member of the GTP-binding Rho protein family, inhibits Ca(2+)-sensitization by specifically interfering with a RhoA/Rho-activated kinases-dependent mechanism in smooth muscle. Guanosine Triphosphate 28-31 ras homolog family member A Rattus norvegicus 125-129 16847745-3 2006 Because the RhoA pathway is involved in neurite outgrowth, Rho-associated kinases (ROCKs), downstream effectors of GTP-bound Rho, are potentially important targets for axonal repair strategies in CNS injuries. Guanosine Triphosphate 115-118 ras homolog family member A Rattus norvegicus 12-16 15310556-6 2004 We further examined effects of NO on levels of GTP-bound RhoA and ROK membrane translocation as indexes of enzyme activity in arteries from each group. Guanosine Triphosphate 47-50 ras homolog family member A Rattus norvegicus 57-61 15890975-7 2005 In addition, stimulation of PKG leads to the accumulation of GTP-bound RhoA in the cytoplasm. Guanosine Triphosphate 61-64 ras homolog family member A Rattus norvegicus 71-75 11557735-4 2001 An increase in active GTP-bound RhoA was detected in aortic homogenates by affinity precipitation with the RhoA effector rhotekin and by examining RhoA-[(35)S]GTPgammaS binding. Guanosine Triphosphate 22-25 ras homolog family member A Rattus norvegicus 32-36 12964001-5 2003 Fluvastatin inhibited LPA-induced translocation of RhoA protein from the cytosol to the membrane and RhoA activation which was measured by pull-down assay for GTP-bound RhoA. Guanosine Triphosphate 159-162 ras homolog family member A Rattus norvegicus 51-55 12964001-5 2003 Fluvastatin inhibited LPA-induced translocation of RhoA protein from the cytosol to the membrane and RhoA activation which was measured by pull-down assay for GTP-bound RhoA. Guanosine Triphosphate 159-162 ras homolog family member A Rattus norvegicus 101-105 12964001-5 2003 Fluvastatin inhibited LPA-induced translocation of RhoA protein from the cytosol to the membrane and RhoA activation which was measured by pull-down assay for GTP-bound RhoA. Guanosine Triphosphate 159-162 ras homolog family member A Rattus norvegicus 101-105 12960549-4 2003 METHODS: GTP gamma S-induced contraction of rat aortic endothelium-denuded rings was measured using an isometric force transducer, and GTP gamma S-stimulated membrane translocation of RhoA and Rock-2 in smooth muscle cells was detected with Western blotting in the presence and absence of sevoflurane. Guanosine Triphosphate 135-138 ras homolog family member A Rattus norvegicus 184-188 12960549-7 2003 GTP gamma S (10(-4) m) stimulated the translocation of RhoA and Rock-2 from the cytosol to the membrane, which was sustained for more than 60 min. Guanosine Triphosphate 0-3 ras homolog family member A Rattus norvegicus 55-59 12960549-8 2003 Sevoflurane (1.7, 3.4, and 5.1%) concentration dependently inhibited the GTP gamma S-induced constriction of rat aortic smooth muscle with a reduction of constriction of 52-75% (P < 0.01, n = 8), and attenuated the translocation of RhoA and Rock-2 by 31-66% and 34-78%, respectively (P < 0.05-0.01, respectively; n = 4). Guanosine Triphosphate 73-76 ras homolog family member A Rattus norvegicus 235-239 12860969-3 2003 Here we detect GTP-bound RhoA in spinal cord homogenates and report that spinal cord injury (SCI) in both rats and mice activates RhoA over 10-fold in the absence of changes in RhoA expression. Guanosine Triphosphate 15-18 ras homolog family member A Rattus norvegicus 25-29 14713118-2 2003 Intracellular signaling via the small GTP-binding protein RhoA and its downstream effector Rho-kinase plays a role in regulating diverse cellular functions, including cell contraction, migration, gene expression, proliferation, and differentiation. Guanosine Triphosphate 38-41 ras homolog family member A Rattus norvegicus 58-62 11557735-4 2001 An increase in active GTP-bound RhoA was detected in aortic homogenates by affinity precipitation with the RhoA effector rhotekin and by examining RhoA-[(35)S]GTPgammaS binding. Guanosine Triphosphate 22-25 ras homolog family member A Rattus norvegicus 107-111 11557735-4 2001 An increase in active GTP-bound RhoA was detected in aortic homogenates by affinity precipitation with the RhoA effector rhotekin and by examining RhoA-[(35)S]GTPgammaS binding. Guanosine Triphosphate 22-25 ras homolog family member A Rattus norvegicus 107-111 10843871-1 2000 The low-molecular-weight GTP-binding protein RhoA mediates hypertrophic growth and atrial natriuretic factor (ANF) gene expression in neonatal rat ventricular myocytes. Guanosine Triphosphate 25-28 ras homolog family member A Rattus norvegicus 45-49 11311142-4 2001 Selection with phage display of different peptides of rPLD(1) confirmed that GTP-bound RhoA interacted with a site in the amino acid sequence 873-1024 at the C-terminus of rPLD(1). Guanosine Triphosphate 77-80 ras homolog family member A Rattus norvegicus 87-91 11311142-5 2001 RhoA also associated with this peptide in a GTP-dependent manner in COS-7 cell lysates and the peptide inhibited RhoA stimulation of PLD activity in membranes from COS-7 cells expressing rPLD(1). Guanosine Triphosphate 44-47 ras homolog family member A Rattus norvegicus 0-4 11068034-4 2000 Moreover, pull-down assays for GTP-bound, active RhoA demonstrated that the blockage by cAMP-elevating agents of morphological changes leading to the migration was probably mediated through inhibiting RhoA activation. Guanosine Triphosphate 31-34 ras homolog family member A Rattus norvegicus 49-53 11068034-4 2000 Moreover, pull-down assays for GTP-bound, active RhoA demonstrated that the blockage by cAMP-elevating agents of morphological changes leading to the migration was probably mediated through inhibiting RhoA activation. Guanosine Triphosphate 31-34 ras homolog family member A Rattus norvegicus 201-205 10486357-7 1999 The cytoskeletal protein actin, a component of focal adhesion plaque protein, vinculin, and the small GTP-binding proteins RhoA and Rac1 were detected by immunohistochemistry in the cells located at the margin of and remote from the wound. Guanosine Triphosphate 102-105 ras homolog family member A Rattus norvegicus 123-127 9495548-0 1997 Gene expression of the small GTP-binding proteins RhoA, RhoB, Rac1, and Cdc42 in adult rat brain. Guanosine Triphosphate 29-32 ras homolog family member A Rattus norvegicus 50-54 10463591-11 1999 Changes in the activated state of the small GTP-binding protein Rho A and its associated proteins were seen but only after 3 h after PHx. Guanosine Triphosphate 44-47 ras homolog family member A Rattus norvegicus 64-69 10381538-0 1999 Differential expression of the small GTP-binding proteins RhoA, RhoB, Cdc42u and Cdc42b in developing rat neocortex. Guanosine Triphosphate 37-40 ras homolog family member A Rattus norvegicus 58-62 10381538-6 1999 Our findings of a differential expression of the small GTP-binding proteins RhoA, RhoB, Cdc42u and Cdc42b in developing brain neocortex suggest isoform specific functions during neurogenesis and differentiation. Guanosine Triphosphate 55-58 ras homolog family member A Rattus norvegicus 76-80 9666307-9 1998 Taken together, these results suggest that the GTP gamma S-bound forms of Cdc42 and RhoA are maintained in a conformation that protects them from proteases found in many cell membranes. Guanosine Triphosphate 47-50 ras homolog family member A Rattus norvegicus 84-88 10198335-13 1999 We concluded that the small GTP-binding protein RhoA p21 exists in pancreatic acini and appears to be involved in the mediation of pancreatic enzyme secretion evoked by CCK and carbachol. Guanosine Triphosphate 28-31 ras homolog family member A Rattus norvegicus 48-52 9666307-5 1998 In addition, Cdc42 and RhoA associated with brush border membranes were largely resistant to increased proteolytic degradation induced by membrane treatment with the denaturing reagent urea as well as to added trypsin when incubated in the presence of GTP gamma S. In brush border membranes, the resistance to endo- and exo-genous proteolytic activity conferred by GTP gamma S was usually lower for RhoA than for Cdc42. Guanosine Triphosphate 252-255 ras homolog family member A Rattus norvegicus 23-27 9666307-5 1998 In addition, Cdc42 and RhoA associated with brush border membranes were largely resistant to increased proteolytic degradation induced by membrane treatment with the denaturing reagent urea as well as to added trypsin when incubated in the presence of GTP gamma S. In brush border membranes, the resistance to endo- and exo-genous proteolytic activity conferred by GTP gamma S was usually lower for RhoA than for Cdc42. Guanosine Triphosphate 252-255 ras homolog family member A Rattus norvegicus 399-403 9666307-5 1998 In addition, Cdc42 and RhoA associated with brush border membranes were largely resistant to increased proteolytic degradation induced by membrane treatment with the denaturing reagent urea as well as to added trypsin when incubated in the presence of GTP gamma S. In brush border membranes, the resistance to endo- and exo-genous proteolytic activity conferred by GTP gamma S was usually lower for RhoA than for Cdc42. Guanosine Triphosphate 365-368 ras homolog family member A Rattus norvegicus 23-27 8870680-5 1996 However, in extracts from crude membranes both mARF and RhoA enhanced the stimulation by GTP[S], with high specific activities of PLD being observed in all tissues except muscle. Guanosine Triphosphate 89-92 ras homolog family member A Rattus norvegicus 56-60 7493923-3 1995 We now report the isolation of a rat cDNA encoding a 150-kDa protein, which specifically binds RhoA in its GTP form and contains an N-terminal serine/threonine kinase domain highly related to the human myotonic dystrophy kinase and a cysteine-rich domain toward the C terminus. Guanosine Triphosphate 107-110 ras homolog family member A Rattus norvegicus 95-99 7929302-0 1994 Activation of rat liver phospholipase D by the small GTP-binding protein RhoA. Guanosine Triphosphate 53-56 ras homolog family member A Rattus norvegicus 73-77 7929302-5 1994 Full reconstitution of GTP gamma S-stimulated phospholipase D in RhoGDI-washed membranes was achieved with recombinant RhoA. Guanosine Triphosphate 23-26 ras homolog family member A Rattus norvegicus 119-123 7929302-9 1994 These findings support a role for GTP-binding proteins of the Rho family in the activation of membrane-associated phospholipase D and implicate RhoA as the major protein involved. Guanosine Triphosphate 34-37 ras homolog family member A Rattus norvegicus 144-148