PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 14525525-2 2003 To date, two isoforms of 11 beta HSD have been cloned: 11 beta HSD1 acts predominantly as an NADP(H)-dependent reductase to generate active cortisol or corticosterone, and 11 beta HSD2 is a high affinity NAD(+)-dependent enzyme that catalyses the enzymatic inactivation of glucocorticoids. NAD 204-210 hydroxysteroid 11-beta dehydrogenase 2 Homo sapiens 180-184 15947887-3 2005 In epithelial tissues the enzyme 11beta hydroxysteroid dehydrogenase Type 2 (11betaHSD2) allows aldosterone to selectively activate MR, by converting cortisol to cortisone and NAD to NADH. NAD 176-179 hydroxysteroid 11-beta dehydrogenase 2 Homo sapiens 33-75 15947887-3 2005 In epithelial tissues the enzyme 11beta hydroxysteroid dehydrogenase Type 2 (11betaHSD2) allows aldosterone to selectively activate MR, by converting cortisol to cortisone and NAD to NADH. NAD 183-187 hydroxysteroid 11-beta dehydrogenase 2 Homo sapiens 33-75 11904232-8 2002 In addition, prior incubation of human placental microsomes with NAD(+) (cofactor) but not cortisol (substrate) resulted in a concentration-dependent increase (EC(50)=8 microM) in 11 beta-HSD2 activity, indicating that binding of NAD(+) to the microsomal 11 beta-HSD2 facilitated the conversion of cortisol to cortisone. NAD 65-71 hydroxysteroid 11-beta dehydrogenase 2 Homo sapiens 180-192 11904232-8 2002 In addition, prior incubation of human placental microsomes with NAD(+) (cofactor) but not cortisol (substrate) resulted in a concentration-dependent increase (EC(50)=8 microM) in 11 beta-HSD2 activity, indicating that binding of NAD(+) to the microsomal 11 beta-HSD2 facilitated the conversion of cortisol to cortisone. NAD 230-236 hydroxysteroid 11-beta dehydrogenase 2 Homo sapiens 180-192 11904232-9 2002 Thus, this finding substantiates the previously proposed concept that a compulsorily ordered ternary complex mechanism may operate for 11 beta-HSD2, with NAD(+) binding first, followed by a conformational change allowing cortisol binding with high affinity. NAD 154-160 hydroxysteroid 11-beta dehydrogenase 2 Homo sapiens 135-147 12943516-3 2003 In contrast, 11beta-HSD2 is a NAD-dependent dehydrogenase inactivating cortisol to cortisone, thereby protecting the mineralocorticoid receptor from occupation by cortisol. NAD 30-33 hydroxysteroid 11-beta dehydrogenase 2 Homo sapiens 13-24 12901862-5 2003 The sulfhydryl protecting reagent dithiothreitol blocked thiram-induced inhibition and NAD+ partially protected 11 beta-HSD2 activity, indicating that DTCs act at the cofactor-binding site. NAD 87-91 hydroxysteroid 11-beta dehydrogenase 2 Homo sapiens 112-124 12901862-6 2003 A 3D-model of 11 beta-HSD2 identified Cys90 in the NAD(+)-binding site as a likely target of DTCs, which was supported by a 99% reduced activity of mutant Cys90 to serine. NAD 51-57 hydroxysteroid 11-beta dehydrogenase 2 Homo sapiens 14-26 12706305-0 2003 Glutamate-115 renders specificity of human 11beta-hydroxysteroid dehydrogenase type 2 for the cofactor NAD+. NAD 103-107 hydroxysteroid 11-beta dehydrogenase 2 Homo sapiens 43-85 12706305-8 2003 Thus, predictions using the 3D-model combined with analysis of mutants allowed the identification of residues critical for NAD(+)-dependent activity of 11beta-HSD2. NAD 123-129 hydroxysteroid 11-beta dehydrogenase 2 Homo sapiens 152-163 11904232-8 2002 In addition, prior incubation of human placental microsomes with NAD(+) (cofactor) but not cortisol (substrate) resulted in a concentration-dependent increase (EC(50)=8 microM) in 11 beta-HSD2 activity, indicating that binding of NAD(+) to the microsomal 11 beta-HSD2 facilitated the conversion of cortisol to cortisone. NAD 65-71 hydroxysteroid 11-beta dehydrogenase 2 Homo sapiens 244-267 11604236-4 2001 Ishikawa cells contain an NAD dependent enzyme migrating at 41 kDa on Western blots, consistent with the presence of the glucocorticoid-inactivating enzyme 11 beta HSD2, while the NADP dependent 11 beta HSD1 is barely detectable. NAD 26-29 hydroxysteroid 11-beta dehydrogenase 2 Homo sapiens 121-168 11696370-2 2001 We observed (i) NAD-dependent enzyme activity and mRNA for 11beta-HSD2, but not 11beta-HSD1, (ii) increasing 11beta-HSD2 activity with increasing degree of differentiation and (iii) a concentration-dependent down-regulation by TNF-alpha, phorbol myristate acetate (PMA) or glucose of activity and mRNA of 11beta-HSD2. NAD 16-19 hydroxysteroid 11-beta dehydrogenase 2 Homo sapiens 59-70 11350048-5 2001 Moreover, the Ca2+-induced inhibition does not appear to involve altered cofactor (NAD+) binding since the inhibition of microsomal 11beta-HSD2 activity by a sub-maximal concentration of free Ca2+ was not overcome by increasing the concentration of NAD+. NAD 249-253 hydroxysteroid 11-beta dehydrogenase 2 Homo sapiens 132-143 10232052-3 1999 11 beta-HSD2 is a high affinity NAD-dependent dehydrogenase that protects the mineralocorticoid receptor from glucocorticoid excess; mutations in the HSD11B2 gene explain an inherited form of hypertension, the syndrome of apparent mineralocorticoid excess in which cortisol acts as a potent mineralocorticoid. NAD 32-35 hydroxysteroid 11-beta dehydrogenase 2 Homo sapiens 150-157 10406076-2 1999 Two different 11 beta-HSD isoforms exist: a low-affinity NADP-dependent dehydrogenase/oxoreductase (11 beta-HSD1) and a high-affinity NAD-dependent dehydrogenase (11 beta-HSD2). NAD 57-60 hydroxysteroid 11-beta dehydrogenase 2 Homo sapiens 14-21 11279298-6 2001 Enzyme activity studies confirmed the presence of a high-affinity, NAD-dependent dehydrogenase activity (K(m) 137 nmol/l and V(max) 128 pmol E/h/mg protein), indicative of the 11 beta-HSD2 isoform. NAD 67-70 hydroxysteroid 11-beta dehydrogenase 2 Homo sapiens 176-188 11469811-3 2001 In contrast, 11beta-HSD2 acts exclusively as an NAD-dependent dehydrogenase inactivating cortisol to cortisone, thereby protecting the mineralocorticoid receptor from occupation by cortisol. NAD 48-51 hydroxysteroid 11-beta dehydrogenase 2 Homo sapiens 13-24 9000459-6 1997 11beta-HSD-2 is a much higher affinity, NAD-dependent, exclusive 11beta-dehydrogenase (glucocorticoid inactivating enzyme), which, when colocalized with otherwise nonselective mineralocorticoid receptors (MR), ensures selective access for aldosterone in vivo. NAD 40-43 hydroxysteroid 11-beta dehydrogenase 2 Homo sapiens 0-12 9828181-5 1998 The 11beta-HSD activity in the placenta was characteristic of 11beta-HSD2 in that it possessed only dehydrogenase activity that was NAD-dependent and had a high affinity for cortisol (Km = 134 nM). NAD 132-135 hydroxysteroid 11-beta dehydrogenase 2 Homo sapiens 62-73 9291187-2 1997 NAD-dependent 11beta-HSD is expressed at high levels in the distal nephron and contributes to mineralocorticoid specificity in that region. NAD 0-3 hydroxysteroid 11-beta dehydrogenase 2 Homo sapiens 14-24 9291187-3 1997 The present studies determined whether N-glycosylation is necessary for the activity of NAD-dependent 11beta-HSD (11beta-HSD2). NAD 88-91 hydroxysteroid 11-beta dehydrogenase 2 Homo sapiens 102-112 9291187-3 1997 The present studies determined whether N-glycosylation is necessary for the activity of NAD-dependent 11beta-HSD (11beta-HSD2). NAD 88-91 hydroxysteroid 11-beta dehydrogenase 2 Homo sapiens 114-125 9135568-3 1997 We have developed a gas chromatographic mass spectrometric assay for dexamethasone and 11-dehydrodexamethasone and have confirmed in vitro that dexamethasone is a substrate for 11 beta-HSD2 but not 11 beta-HSD1 (conversion to 11-dehydrodexamethasone 0.6 +/- 0.3% in homogenates of rat liver with NADP+ for 11 beta-HSD1, and 29.4 +/- 10.3% and 40.0 +/- 2.0% in homogenates of rat and human kidney respectively with NAD+ for 11 beta-HSD2). NAD 414-418 hydroxysteroid 11-beta dehydrogenase 2 Homo sapiens 177-184 9723880-2 1998 The results demonstrated a predominant expression of the NAD-dependent dehydrogenase isoform (11beta-HSD2) over the NADP-dependent oxoreductase (11beta-HSD1). NAD 57-60 hydroxysteroid 11-beta dehydrogenase 2 Homo sapiens 94-105 9426159-5 1997 Stably transfected CHO cell lines expressed high, NAD-dependent, unidirectional 11 beta-HSD2 activity. NAD 50-53 hydroxysteroid 11-beta dehydrogenase 2 Homo sapiens 80-92 8782085-4 1996 The 11 beta-HSD activity in JEG-3 cell homogenates required NAD+ as cofactor with NADP+ ineffective and demonstrated a high affinity for cortisol (apparent Km 31 nM). NAD 60-64 hydroxysteroid 11-beta dehydrogenase 2 Homo sapiens 4-11 8938587-3 1996 Two kinetically distinct isoforms of this enzyme have been characterized in both rodents and man; a low-affinity NADP(H)-dependent enzyme (11 beta-HSD1) which predominantly acts as an oxoreductase and, more recently, a high-affinity NAD-dependent uni-directional dehydrogenase (11 beta-HSD2). NAD 113-116 hydroxysteroid 11-beta dehydrogenase 2 Homo sapiens 278-290 8852827-4 1996 11 beta-HSD-2 is NAD(+)-dependent, has a Km in the nanomolar range, exhibits only oxidase activity, and colocalizes with the MR in the kidney, so is likely to serve as the gatekeeper for the MR. We have further characterized 11 beta-HSD activity in JEG-3 cells, a cell line derived from a human choriocarcinoma which was reported to have only the high affinity, NAD(+)-dependent 11 beta-HSD-2. NAD 17-23 hydroxysteroid 11-beta dehydrogenase 2 Homo sapiens 0-13 8852827-4 1996 11 beta-HSD-2 is NAD(+)-dependent, has a Km in the nanomolar range, exhibits only oxidase activity, and colocalizes with the MR in the kidney, so is likely to serve as the gatekeeper for the MR. We have further characterized 11 beta-HSD activity in JEG-3 cells, a cell line derived from a human choriocarcinoma which was reported to have only the high affinity, NAD(+)-dependent 11 beta-HSD-2. NAD 362-368 hydroxysteroid 11-beta dehydrogenase 2 Homo sapiens 0-13 8611186-8 1996 Affinity chromatography and affinity labelling studies suggest that 11 beta-HSD2 has a compulsory ordered mechanism, with NAD+ binding first, followed by a conformational change allowing glucocorticoid binding with high affinity. NAD 122-126 hydroxysteroid 11-beta dehydrogenase 2 Homo sapiens 68-80 8770980-2 1996 Both NADP, and NAD-dependent isoforms of 11 beta-HSD have been described. NAD 5-8 hydroxysteroid 11-beta dehydrogenase 2 Homo sapiens 41-48 8538347-8 1996 NAD-dependent 11 beta-HSD activity was severely attenuated in the stillbirth placenta compared with control placental tissue, and no 11 beta-HSD immunostaining was observed in this placenta with antisera derived against a C-terminal 11 beta-HSD2 peptide sequence. NAD 0-3 hydroxysteroid 11-beta dehydrogenase 2 Homo sapiens 14-21 8613810-3 1996 Two isoforms exist: 11 beta-HSD1, a bidirectional NADPH-dependent enzyme, and 11 beta-HSD2, an NAD(+)-dependent exclusive 11 beta-dehydrogenase (corticosterone-inactivating enzyme). NAD 95-101 hydroxysteroid 11-beta dehydrogenase 2 Homo sapiens 50-90 8770980-3 1996 An NAD-dependent isoform of 11 beta-HSD (11 beta-HSD2) was recently cloned from human kidney. NAD 3-6 hydroxysteroid 11-beta dehydrogenase 2 Homo sapiens 28-35 8770980-3 1996 An NAD-dependent isoform of 11 beta-HSD (11 beta-HSD2) was recently cloned from human kidney. NAD 3-6 hydroxysteroid 11-beta dehydrogenase 2 Homo sapiens 41-53 8770980-11 1996 These results support the view that the NAD-dependent isoform of 11 beta-HSD (11 beta-HSD2) provides mineralocorticoid specificity by inactivating glucocorticoids in an autocrine fashion. NAD 40-43 hydroxysteroid 11-beta dehydrogenase 2 Homo sapiens 65-72 8770980-11 1996 These results support the view that the NAD-dependent isoform of 11 beta-HSD (11 beta-HSD2) provides mineralocorticoid specificity by inactivating glucocorticoids in an autocrine fashion. NAD 40-43 hydroxysteroid 11-beta dehydrogenase 2 Homo sapiens 78-90 7656579-1 1995 The enzyme 11 beta-hydroxysteroid dehydrogenase type 2 (11 beta HSD2) is an NAD-dependent, high-affinity isoform that potently inactivates glucocorticoids. NAD 76-79 hydroxysteroid 11-beta dehydrogenase 2 Homo sapiens 4-68 8547170-3 1995 In the kidney it is the NAD dependent high affinity isoform (11 beta-HSD2) which is thought to endow specificity on the receptor. NAD 24-27 hydroxysteroid 11-beta dehydrogenase 2 Homo sapiens 61-73 7859916-2 1994 A ubiquitous low affinity NADP+ dependent enzyme (11 beta HSD1) and a tissue specific, high affinity NAD+ dependent form (11 beta HSD2) of 11 beta HSD exist. NAD 101-105 hydroxysteroid 11-beta dehydrogenase 2 Homo sapiens 122-134 21333409-6 2011 Furthermore, the ligand-binding site (LBS) volume was calculated to be 845 A(3), which suggests that the LBS of 11betaHSD2 is sufficiently large to contain cofactors and substrates (ligands), such as NAD(+) and cortisol. NAD 200-206 hydroxysteroid 11-beta dehydrogenase 2 Homo sapiens 112-122 28709706-2 2017 Kidney 11beta-hydroxysteroid dehydrogenase 2 (11beta-HSD2) is an NAD+-dependent oxidase that inactivates glucocorticoid cortisol (human) or corticosterone (rodents) into biologically inert 11 keto glucocorticoids. NAD 65-68 hydroxysteroid 11-beta dehydrogenase 2 Homo sapiens 7-44 28709706-2 2017 Kidney 11beta-hydroxysteroid dehydrogenase 2 (11beta-HSD2) is an NAD+-dependent oxidase that inactivates glucocorticoid cortisol (human) or corticosterone (rodents) into biologically inert 11 keto glucocorticoids. NAD 65-68 hydroxysteroid 11-beta dehydrogenase 2 Homo sapiens 46-57 21667072-0 2012 Molecular docking and structural analysis of cofactor-protein interaction between NAD+ and 11beta-hydroxysteroid dehydrogenase type 2. NAD 82-86 hydroxysteroid 11-beta dehydrogenase 2 Homo sapiens 91-133 21667072-4 2012 Our present study also revealed that Asp91, Phe94, Tyr232 and Thr267 could be of importance in the interaction between NAD(+) and 11betaHSD2. NAD 119-125 hydroxysteroid 11-beta dehydrogenase 2 Homo sapiens 130-140 21667072-6 2012 The present study proposes the latest models for 11betaHSD2 and its cofactor NAD(+), and to the best of our knowledge, this is the first report of a m11betaHSD2 model with NAD(+). NAD 77-83 hydroxysteroid 11-beta dehydrogenase 2 Homo sapiens 49-59 21667072-6 2012 The present study proposes the latest models for 11betaHSD2 and its cofactor NAD(+), and to the best of our knowledge, this is the first report of a m11betaHSD2 model with NAD(+). NAD 172-178 hydroxysteroid 11-beta dehydrogenase 2 Homo sapiens 49-59 27431412-6 2016 The 11beta-HSD2 isoform, which catalyzes nicotinamide adenine dinucleotide+-dependent inactivation of cortisol/corticosterone to cortisone/11-DHC, is highly expressed in distal nephrons, the colon, sweat glands, and the placenta. NAD 41-75 hydroxysteroid 11-beta dehydrogenase 2 Homo sapiens 4-15 20219674-3 2010 Using NAD(+), 11beta-HSD2 from testicular microsomes oxidized 11-OHT with apparent K(m) 56+/-4nM and V(max) 55+/-6pmol/h/mgprotein values. NAD 6-12 hydroxysteroid 11-beta dehydrogenase 2 Homo sapiens 14-25