PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 32058033-12 2020 Both EPA and DHA (50 muM) significantly decreased cyclooxygenase (COX)-2 protein. Docosahexaenoic Acids 13-16 latexin Homo sapiens 21-24 32058033-10 2020 ALA, SDA and DHA (50 muM) all reduced adhesion of THP-1 monocytes to EA.hy926 cells. Docosahexaenoic Acids 13-16 latexin Homo sapiens 21-24 30669396-10 2019 RNA-sequencing analysis revealed that some muscle-related genes were significantly downregulated following EPA or DHA (50 muM) treatment, including insulin-like growth factor 2 (IGF-2), troponin T3 (Tnnt3), myoglobin (Mb), myosin light chain phosphorylatable fast skeletal muscle (Mylpf) and myosin heavy polypeptide 3 (Myh3). Docosahexaenoic Acids 114-117 latexin Homo sapiens 122-125 30669396-8 2019 Cell viability and proliferation was significantly inhibited after incubation with EPA (50 and 100 muM) or DHA (100 muM). Docosahexaenoic Acids 107-110 latexin Homo sapiens 116-119 30669396-12 2019 We found that EPA and DHA (50 muM) decreased the phosphorylation levels of ERK1/2 and Akt in C2C12 myoblasts. Docosahexaenoic Acids 22-25 latexin Homo sapiens 30-33 27728769-9 2016 This work contributes to an understanding of the physical changes induced in a lipid bilayer as a consequence of its exposure to different DHA concentrations (from 50 to 200 muM). Docosahexaenoic Acids 139-142 latexin Homo sapiens 174-177 27044314-6 2016 RESULTS: 50 muM, 10 muM EPA and 50 muM EPA + DHA decreased the expression of genes involved in the NF-kappaB pathway (MAPK, AKT1, and NFKB). Docosahexaenoic Acids 45-48 latexin Homo sapiens 12-15 27044314-7 2016 Treatment with 50 muM, 10 muM EPA, 50 muM DHA and EPA + DHA decreased expression levels of cytokines genes IL1Beta and MCP1. Docosahexaenoic Acids 56-59 latexin Homo sapiens 18-21 27565090-3 2016 MTT assay showed that DHA and EPA still exhibited no cytotoxicity to human oral tissue cells when the concentration came to 100 muM and 200 muM, respectively. Docosahexaenoic Acids 22-25 latexin Homo sapiens 128-131 27565090-3 2016 MTT assay showed that DHA and EPA still exhibited no cytotoxicity to human oral tissue cells when the concentration came to 100 muM and 200 muM, respectively. Docosahexaenoic Acids 22-25 latexin Homo sapiens 140-143 27565090-8 2016 For exponential-phase bacteria, 100 muM DHA or EPA completely killed P. gingivalis and significantly decreased the viable counts of F. nucleatum. Docosahexaenoic Acids 40-43 latexin Homo sapiens 36-39 27565090-10 2016 Besides, the viability and the thickness of mature P. gingivalis biofilm, together with the viability of mature F. nucleatum biofilm were both significantly decreased in the presence of 100 muM DHA or EPA. Docosahexaenoic Acids 194-197 latexin Homo sapiens 190-193 26657646-6 2016 In contrast, docosahexaenoic acid treatment (6 muM) increased docosahexaenoic acid content and attenuated glucocorticoid hormone corticosterone (200 muM)-induced cell death (72 hours) in cortical cultures. Docosahexaenoic Acids 13-33 latexin Homo sapiens 47-50 26657646-6 2016 In contrast, docosahexaenoic acid treatment (6 muM) increased docosahexaenoic acid content and attenuated glucocorticoid hormone corticosterone (200 muM)-induced cell death (72 hours) in cortical cultures. Docosahexaenoic Acids 13-33 latexin Homo sapiens 149-152 26657646-6 2016 In contrast, docosahexaenoic acid treatment (6 muM) increased docosahexaenoic acid content and attenuated glucocorticoid hormone corticosterone (200 muM)-induced cell death (72 hours) in cortical cultures. Docosahexaenoic Acids 62-82 latexin Homo sapiens 47-50 26657646-8 2016 Furthermore, docosahexaenoic acid (6 muM) reversed glucocorticoid hormone corticosterone-induced neuronal apoptosis as assessed by terminal deoxynucleotidyl transferase-mediated nick-end labeling and attenuated glucocorticoid hormone corticosterone-induced reductions in brain derived neurotrophic factor mRNA expression in these cultures. Docosahexaenoic Acids 13-33 latexin Homo sapiens 37-40 27041074-6 2016 In MCF-7 cells, liposomal DHA (IC50 38.8 muM) significantly reduced cell viability more effectively than free DHA (IC50 72.5 muM, p = 0.0017). Docosahexaenoic Acids 26-29 latexin Homo sapiens 41-44 27041074-6 2016 In MCF-7 cells, liposomal DHA (IC50 38.8 muM) significantly reduced cell viability more effectively than free DHA (IC50 72.5 muM, p = 0.0017). Docosahexaenoic Acids 26-29 latexin Homo sapiens 125-128 27044314-7 2016 Treatment with 50 muM, 10 muM EPA, 50 muM DHA and EPA + DHA decreased expression levels of cytokines genes IL1Beta and MCP1. Docosahexaenoic Acids 56-59 latexin Homo sapiens 26-29 27044314-7 2016 Treatment with 50 muM, 10 muM EPA, 50 muM DHA and EPA + DHA decreased expression levels of cytokines genes IL1Beta and MCP1. Docosahexaenoic Acids 56-59 latexin Homo sapiens 26-29 27044314-7 2016 Treatment with 50 muM, 10 muM EPA, 50 muM DHA and EPA + DHA decreased expression levels of cytokines genes IL1Beta and MCP1. Docosahexaenoic Acids 42-45 latexin Homo sapiens 18-21 27044314-10 2016 50 muM of DHA and EPA + DHA inhibited PTGS2 expression when the two concentrations of EPA, 50 muM DHA and EPA + DHA inhibited ALOX5 expression. Docosahexaenoic Acids 10-13 latexin Homo sapiens 3-6 26671842-7 2016 After a 72 h treatment with 150 muM DHA and EPA, or their combination (75/75 muM), growth rates were inhibited by 80.3 +- 5.5%, 79.3 +- 5% and 71.1 +- 1%, respectively, compared to untreated cells. Docosahexaenoic Acids 36-39 latexin Homo sapiens 32-35 27044314-10 2016 50 muM of DHA and EPA + DHA inhibited PTGS2 expression when the two concentrations of EPA, 50 muM DHA and EPA + DHA inhibited ALOX5 expression. Docosahexaenoic Acids 10-13 latexin Homo sapiens 94-97 27044314-10 2016 50 muM of DHA and EPA + DHA inhibited PTGS2 expression when the two concentrations of EPA, 50 muM DHA and EPA + DHA inhibited ALOX5 expression. Docosahexaenoic Acids 24-27 latexin Homo sapiens 3-6 27044314-10 2016 50 muM of DHA and EPA + DHA inhibited PTGS2 expression when the two concentrations of EPA, 50 muM DHA and EPA + DHA inhibited ALOX5 expression. Docosahexaenoic Acids 24-27 latexin Homo sapiens 94-97 27044314-10 2016 50 muM of DHA and EPA + DHA inhibited PTGS2 expression when the two concentrations of EPA, 50 muM DHA and EPA + DHA inhibited ALOX5 expression. Docosahexaenoic Acids 24-27 latexin Homo sapiens 3-6 27044314-10 2016 50 muM of DHA and EPA + DHA inhibited PTGS2 expression when the two concentrations of EPA, 50 muM DHA and EPA + DHA inhibited ALOX5 expression. Docosahexaenoic Acids 24-27 latexin Homo sapiens 94-97 27044314-10 2016 50 muM of DHA and EPA + DHA inhibited PTGS2 expression when the two concentrations of EPA, 50 muM DHA and EPA + DHA inhibited ALOX5 expression. Docosahexaenoic Acids 24-27 latexin Homo sapiens 3-6 27044314-10 2016 50 muM of DHA and EPA + DHA inhibited PTGS2 expression when the two concentrations of EPA, 50 muM DHA and EPA + DHA inhibited ALOX5 expression. Docosahexaenoic Acids 24-27 latexin Homo sapiens 94-97 27044314-13 2016 Both concentration of EPA and 50 muM DHA decreased NOS2 expression. Docosahexaenoic Acids 37-40 latexin Homo sapiens 33-36 26671842-7 2016 After a 72 h treatment with 150 muM DHA and EPA, or their combination (75/75 muM), growth rates were inhibited by 80.3 +- 5.5%, 79.3 +- 5% and 71.1 +- 1%, respectively, compared to untreated cells. Docosahexaenoic Acids 36-39 latexin Homo sapiens 77-80 26671842-8 2016 We also found that treatment for 48 h with 100 muM DHA and EPA, or their combination (50/50 muM), resulted in 2.9-, 3- and 2.6-fold increases in caspase-3 activation, as well as 54, 62.4 and 100% decreases in survivin mRNA expression levels, respectively, compared to untreated cells. Docosahexaenoic Acids 51-54 latexin Homo sapiens 47-50 26671842-8 2016 We also found that treatment for 48 h with 100 muM DHA and EPA, or their combination (50/50 muM), resulted in 2.9-, 3- and 2.6-fold increases in caspase-3 activation, as well as 54, 62.4 and 100% decreases in survivin mRNA expression levels, respectively, compared to untreated cells. Docosahexaenoic Acids 51-54 latexin Homo sapiens 92-95 25889069-12 2015 Changes in synaptic transmission of CA1 pyramidal cells were determined following LPS and DHA (25-50 muM) treatments by recording spontaneous AMPA-mediated miniature excitatory postsynaptic currents (mEPSCs). Docosahexaenoic Acids 90-93 latexin Homo sapiens 101-104 26381084-3 2015 Therefore the aim of the study was to investigate whether EPA and DHA (25-100 muM) are able to reduce human lung cancer cell growth through oxidative stress influence on autophagy and apoptosis. Docosahexaenoic Acids 66-69 latexin Homo sapiens 78-81 25526274-11 2015 RESULTS: U87-MG growth was inhibited with the supplementation of either DHA (ED50 68.3 muM) or lomustine (ED50 68.1 muM); however, growth inhibition was enhanced when U87-MG cells were administered equimolar doses of each compound, resulting in nearly total growth inhibition at 50 muM. Docosahexaenoic Acids 72-75 latexin Homo sapiens 87-90 25526274-12 2015 Gas chromatography analysis of the fatty acid profile in DHA-supplemented U87-MG cells resulted in a linear dose-dependent increase in DHA incorporation (< 60 muM). Docosahexaenoic Acids 57-60 latexin Homo sapiens 162-165 25526274-12 2015 Gas chromatography analysis of the fatty acid profile in DHA-supplemented U87-MG cells resulted in a linear dose-dependent increase in DHA incorporation (< 60 muM). Docosahexaenoic Acids 135-138 latexin Homo sapiens 162-165 25044948-5 2014 DHA at 50 and 100 muM effectively inhibited LA-induced MCP-1 expression and production (p < 0.05) and NF-kappaB activation. Docosahexaenoic Acids 0-3 latexin Homo sapiens 18-21 25504554-4 2015 We observed that cellular viability reduction induced by experimental ischemia as well as cell damage and thiobarbituric acid reactive substances (TBARS) production induced by glutamate (10 mM) were prevented by hippocampal slices pretreated with DHA (5 muM). Docosahexaenoic Acids 247-250 latexin Homo sapiens 254-257 25504554-6 2015 The beneficial effect of DHA against cellular viability reduction induced by OGD and re-oxygenation was blocked with PPADS (3 muM), a nonselective P2X1-5 receptor antagonist as well as with a combination of TNP-APT (100 nM) plus brilliant blue (100 nM), which blocked P2X1, P2X3, P2X2/3, and P2X7 receptors, respectively. Docosahexaenoic Acids 25-28 latexin Homo sapiens 126-129 25044948-6 2014 In addition, the culture medium from LA-stimulated ARPE-19 cells could induce tube formation in choroidal endothelial cells (RF6A), whereas 100 muM DHA inhibited tube formation. Docosahexaenoic Acids 148-151 latexin Homo sapiens 144-147 25044948-7 2014 DHA at 100 muM increased the expression and activity of PPARgamma (p < 0.05). Docosahexaenoic Acids 0-3 latexin Homo sapiens 11-14 25044948-8 2014 Pretreatment with PPARgamma inhibitor (GW9662) abolished the inhibitory effect of DHA (100 muM) on LA-induced IkappaB degradation, p65 translocation, and MCP-1 expression in ARPE-19 cells (p < 0.05), as well as tube formation in RF6A. Docosahexaenoic Acids 82-85 latexin Homo sapiens 91-94 23813449-5 2013 DHA at a concentration of 100 muM induced oxidative stress in term placentas, while at all the three concentrations, it induced oxidative stress in preterm placentas. Docosahexaenoic Acids 0-3 latexin Homo sapiens 30-33 22901690-2 2013 The present results demonstrated that pretreatment with DHA (50 and 100 muM) inhibited tumor necrosis factor-alpha (TNF-alpha)-induced intercellular adhesion molecule 1 (ICAM-1) protein, mRNA expression and promoter activity. Docosahexaenoic Acids 56-59 latexin Homo sapiens 72-75 20725866-5 2011 Cells were supplemented with DHA (25 muM) for 3 days and then subjected to oxidative stress (OS) exerted by hydrogen peroxide. Docosahexaenoic Acids 29-32 latexin Homo sapiens 37-40 23288142-4 2013 We found that TPA (100 ng/ml) induced MMP-9 enzyme activity both dose- and time-dependently, and 200 muM DHA and LA significantly inhibited MMP-9 mRNA and protein expression, enzyme activity, cell migration, and invasion. Docosahexaenoic Acids 105-108 latexin Homo sapiens 101-104 23645098-3 2013 The aim of the study was to test whether DHA can exert a direct protective effect on the elements of the neurovascular unit, such as neurons, glial cells, brain endothelial cells, and pericytes, treated with Abeta42 (15 muM). Docosahexaenoic Acids 41-44 latexin Homo sapiens 220-223 23645098-8 2013 DHA (30 muM) significantly decreased the Abeta42-induced toxic effects in all cell types measured by viability assays, and protected the barrier integrity and functions of brain endothelial cells. Docosahexaenoic Acids 0-3 latexin Homo sapiens 8-11 19190950-3 2009 DHA (50 muM) produced a ROS induction in cells and aggravated the LysoPtdCho-induced oxidative stress. Docosahexaenoic Acids 0-3 latexin Homo sapiens 8-11