PMID-sentid	Pub_year	Sent_text		comp_official_name	comp_offsetprotein_name	organism	prot_offset
22893598-2	2012	The assay is based on the specific interaction of CDK2 with the extrinsic fluorophore 8-anilino-1-naphthalene sulfonate (ANS), which binds to a large allosteric pocket adjacent to the ATP site.	8-anilino-1-naphthalenesulfonic acid	86-119	cyclin dependent kinase 2	Homo sapiens	50-54	
32433863-3	2020	Here, we detail the positive cooperativity between ANS and orthosteric Cdk2 inhibitors dinaciclib and roscovitine, which increase the affinity of ANS toward Cdk2 5-fold to 10-fold, and the relatively noncooperative effects of ATP.	8-anilino-1-naphthalenesulfonic acid	146-149	cyclin dependent kinase 2	Homo sapiens	71-75	
32433863-3	2020	Here, we detail the positive cooperativity between ANS and orthosteric Cdk2 inhibitors dinaciclib and roscovitine, which increase the affinity of ANS toward Cdk2 5-fold to 10-fold, and the relatively noncooperative effects of ATP.	8-anilino-1-naphthalenesulfonic acid	146-149	cyclin dependent kinase 2	Homo sapiens	157-161	
32433863-0	2020	Cooperativity Between Orthosteric Inhibitors and Allosteric Inhibitor 8-Anilino-1-Naphthalene Sulfonic Acid (ANS) in Cyclin-Dependent Kinase 2.	8-anilino-1-naphthalenesulfonic acid	70-107	cyclin dependent kinase 2	Homo sapiens	117-142	
32433863-0	2020	Cooperativity Between Orthosteric Inhibitors and Allosteric Inhibitor 8-Anilino-1-Naphthalene Sulfonic Acid (ANS) in Cyclin-Dependent Kinase 2.	8-anilino-1-naphthalenesulfonic acid	109-112	cyclin dependent kinase 2	Homo sapiens	117-142	
32433863-2	2020	We have previously discovered that 8-anilino-1-naphthalene sulfonic acid (ANS) binds an allosteric pocket in cyclin-dependent kinase 2 (Cdk2).	8-anilino-1-naphthalenesulfonic acid	35-72	cyclin dependent kinase 2	Homo sapiens	109-134	
32433863-2	2020	We have previously discovered that 8-anilino-1-naphthalene sulfonic acid (ANS) binds an allosteric pocket in cyclin-dependent kinase 2 (Cdk2).	8-anilino-1-naphthalenesulfonic acid	35-72	cyclin dependent kinase 2	Homo sapiens	136-140	
32433863-2	2020	We have previously discovered that 8-anilino-1-naphthalene sulfonic acid (ANS) binds an allosteric pocket in cyclin-dependent kinase 2 (Cdk2).	8-anilino-1-naphthalenesulfonic acid	74-77	cyclin dependent kinase 2	Homo sapiens	109-134	
32433863-2	2020	We have previously discovered that 8-anilino-1-naphthalene sulfonic acid (ANS) binds an allosteric pocket in cyclin-dependent kinase 2 (Cdk2).	8-anilino-1-naphthalenesulfonic acid	74-77	cyclin dependent kinase 2	Homo sapiens	136-140	
32433863-3	2020	Here, we detail the positive cooperativity between ANS and orthosteric Cdk2 inhibitors dinaciclib and roscovitine, which increase the affinity of ANS toward Cdk2 5-fold to 10-fold, and the relatively noncooperative effects of ATP.	8-anilino-1-naphthalenesulfonic acid	51-54	cyclin dependent kinase 2	Homo sapiens	157-161	
26685215-8	2016	Second, in 8-anilino-1-naphthalene-sulfonate (ANS)-CDK2 fluorescence assays, preincubation of CDK2 with salicylic acid dose-dependently quenched the fluorescence due to ANS.	8-anilino-1-naphthalenesulfonic acid	11-44	cyclin dependent kinase 2	Homo sapiens	51-55	
26685215-8	2016	Second, in 8-anilino-1-naphthalene-sulfonate (ANS)-CDK2 fluorescence assays, preincubation of CDK2 with salicylic acid dose-dependently quenched the fluorescence due to ANS.	8-anilino-1-naphthalenesulfonic acid	11-44	cyclin dependent kinase 2	Homo sapiens	94-98	
26685215-8	2016	Second, in 8-anilino-1-naphthalene-sulfonate (ANS)-CDK2 fluorescence assays, preincubation of CDK2 with salicylic acid dose-dependently quenched the fluorescence due to ANS.	8-anilino-1-naphthalenesulfonic acid	46-49	cyclin dependent kinase 2	Homo sapiens	51-55	
26685215-8	2016	Second, in 8-anilino-1-naphthalene-sulfonate (ANS)-CDK2 fluorescence assays, preincubation of CDK2 with salicylic acid dose-dependently quenched the fluorescence due to ANS.	8-anilino-1-naphthalenesulfonic acid	46-49	cyclin dependent kinase 2	Homo sapiens	94-98	
26685215-8	2016	Second, in 8-anilino-1-naphthalene-sulfonate (ANS)-CDK2 fluorescence assays, preincubation of CDK2 with salicylic acid dose-dependently quenched the fluorescence due to ANS.	8-anilino-1-naphthalenesulfonic acid	169-172	cyclin dependent kinase 2	Homo sapiens	94-98	
22893598-2	2012	The assay is based on the specific interaction of CDK2 with the extrinsic fluorophore 8-anilino-1-naphthalene sulfonate (ANS), which binds to a large allosteric pocket adjacent to the ATP site.	8-anilino-1-naphthalenesulfonic acid	121-124	cyclin dependent kinase 2	Homo sapiens	50-54	
22893598-3	2012	Hit compounds that displace ANS directly or indirectly from CDK2 are readily classified as ATP site binders or allosteric ligands through the use of staurosporine, which blocks the ATP site without displacing ANS.	8-anilino-1-naphthalenesulfonic acid	28-31	cyclin dependent kinase 2	Homo sapiens	60-64	
22893598-3	2012	Hit compounds that displace ANS directly or indirectly from CDK2 are readily classified as ATP site binders or allosteric ligands through the use of staurosporine, which blocks the ATP site without displacing ANS.	8-anilino-1-naphthalenesulfonic acid	209-212	cyclin dependent kinase 2	Homo sapiens	60-64	
21291269-5	2011	In an effort to identify potential target sites for disruption of the CDK-cyclin interaction, we probed the extrinsic fluorophore 8-anilino-1-naphthalene sulfonate (ANS) with human CDK2 and cyclin A using fluorescence spectroscopy and protein crystallography.	8-anilino-1-naphthalenesulfonic acid	130-163	cyclin dependent kinase 2	Homo sapiens	181-185	
21291269-5	2011	In an effort to identify potential target sites for disruption of the CDK-cyclin interaction, we probed the extrinsic fluorophore 8-anilino-1-naphthalene sulfonate (ANS) with human CDK2 and cyclin A using fluorescence spectroscopy and protein crystallography.	8-anilino-1-naphthalenesulfonic acid	165-168	cyclin dependent kinase 2	Homo sapiens	181-185	
21291269-6	2011	ANS interacts with free CDK2 in a saturation-dependent manner with an apparent K(d) of 37 muM, and cyclin A displaced ANS from CDK2 with an EC(50) value of 0.6 muM.	8-anilino-1-naphthalenesulfonic acid	0-3	cyclin dependent kinase 2	Homo sapiens	24-28	
21291269-6	2011	ANS interacts with free CDK2 in a saturation-dependent manner with an apparent K(d) of 37 muM, and cyclin A displaced ANS from CDK2 with an EC(50) value of 0.6 muM.	8-anilino-1-naphthalenesulfonic acid	118-121	cyclin dependent kinase 2	Homo sapiens	127-131	
21291269-8	2011	Binding of ANS is accompanied by substantial structural changes in CDK2, resulting in a C-helix conformation that is incompatible for cyclin A association.	8-anilino-1-naphthalenesulfonic acid	11-14	cyclin dependent kinase 2	Homo sapiens	67-71	
21291269-9	2011	These findings indicate the potential of the ANS binding pocket as a new target site for allosteric inhibitors disrupting the interaction of CDKs and cyclins.	8-anilino-1-naphthalenesulfonic acid	45-48	cyclin dependent kinase 2	Homo sapiens	141-145